An egg retrieval training device based on an oocyte-cumulus complex mimic

Abstract

The present invention relates to the technical field of reproductive medicine, and particularly to an egg retrieval training device based on an oocyte-cumulus complex mimic, comprising a microscope, a double-well dish and an egg retrieval dish. A third eyepiece is provided on the microscope, and the third eyepiece is used to display image data; an image collector is arranged inside the microscope and is used to capture the internal images of the egg retrieval dish and the double-well dish; a central control module, a timing module and a counting module are arranged inside the microscope, and the central control module is connected to the timing module, the counting module, the image collector and the third eyepiece. The present invention judges the difficulty of egg retrieval through the central control module, evaluates the egg retrieval behavior according to different difficulties, analyzes the egg retrieval operation, controls the timing of the timing module, counts the number of qualified times, and determines whether the trainee is qualified by analyzing the completion time of each stage, the total completion time and the stability, and ranks each trainee and displays the ranking on the third eyepiece.

Description

Technical Field

[0001] The present invention relates to the technical field of reproductive medicine, and particularly relates to an egg retrieval training device based on an oocyte-cumulus-corona complex mimic. Background Art

[0002] In the diagnosis and treatment work of a reproductive medicine center, a very important link is the "egg retrieval surgery" of clinical doctors and the "egg retrieval operation" of embryo laboratory staff; in the daily work of the embryo laboratory, the staff must master the "egg retrieval" operation proficiently; when a female patient who meets the indications for in vitro fertilization undergoes ovulation induction treatment with ovulation induction drugs to make the follicles develop and ovulate, an egg retrieval surgery will be performed. At that time, under the guidance of a vaginal B-ultrasound, the clinical doctor punctures through the vagina to aspirate the target follicles, and then collects the oocyte-cumulus-corona complex in the follicle together with the follicular fluid in a test tube and seals it. Under the premise of ensuring the test tube temperature, it is quickly passed through the window between the egg retrieval operating room and the embryo laboratory to the embryo laboratory staff. The latter pours the liquid in the test tube into a 3003 dish and identifies and retrieves the oocyte-cumulus-corona complex under a microscope.

[0003] The qualified determination for being able to perform the egg retrieval position generally requires continuously retrieving eggs from dozens of patients without missing any oocyte-cumulus-corona complex, and there is also a minimum number requirement for the number of follicles of each patient, such as not less than 8 (for example, if only continuously retrieving eggs from dozens of patients with a total of only 5 oocyte-cumulus-corona complexes without missing any, it is also unqualified and cannot independently undertake the egg retrieval position). There should be no missing retrievals during continuous egg retrieval. Once a missing retrieval occurs, it will be recounted. Moreover, during the retrieval stage, under the premise of ensuring the temperature, it must be fast to reduce the damage of the external environment to the oocytes.

[0004] In reproductive centers with a large diagnosis and treatment scale, there are many clinical egg retrieval patients every day. To improve work efficiency, although the egg retrieval operation is mostly carried out by personnel who are proficient in egg retrieval operations in the embryo laboratory, there are also more opportunities to guide new trainees, increasing the work pressure on the personnel who are proficient in egg retrieval operations in the embryo laboratory; in reproductive centers with a medium or small diagnosis and treatment scale, due to limited patients, the practical operation opportunities for embryo laboratory staff are limited. In this case, it is very difficult for new trainees to get practical operation opportunities, which greatly limits the development of new personnel. Summary of the Invention

[0005] Therefore, the present invention provides an egg retrieval training device based on an oocyte-cumulus-corona complex mimic to overcome the problem of the difficult cultivation of egg retrieval operators in the prior art.

[0006] To achieve the above object, the present invention provides an egg retrieval training device based on an oocyte-cumulus-corona complex mimic, including,

[0007] a microscope, a double-well dish, and an egg retrieval dish. A third eyepiece is provided on the microscope, and the third eyepiece is used to display image data;

[0008] An image collector, which is arranged inside the microscope and is used to capture the internal images of the egg picking dish and the double-well dish;

[0009] A central control module, a timing module and a counting module are arranged inside the microscope. The central control module is connected to the timing module, the counting module, the image collector and the third eyepiece. The central control module divides the egg picking stage into an egg searching stage, an egg picking stage and a transferring stage. According to the judgment of the difficulty of egg picking, the egg picking behavior is evaluated according to different difficulties. By receiving the image signal transmitted by the image collector, the egg picking operation is analyzed, the timing module is controlled to time, the egg searching stage, the egg picking stage and the transferring stage are timed. By controlling the counting module, the qualified times in the egg searching stage, the egg picking stage and the transferring stage are counted. The completion time of each stage, the total completion time and the stability are analyzed to determine whether the training personnel are qualified, and the training personnel are ranked. The timing data of the timing module, the counting data of the counting module, the result of performance judgment and the ranking of the training personnel are displayed on the third eyepiece.

[0010] Further, the central control module inside the microscope stores three difficulty display states, which respectively include, according to the increasing difficulty level

[0011] The first difficulty is the assisted operation state, which is connected to the egg picking operation assisting device and evaluates the egg picking behavior when there is an assisting device for the egg picking operation;

[0012] The second difficulty is the non-assisted operation state, which evaluates the egg picking behavior when there is no assisting device;

[0013] The third difficulty is the interference operation state, which evaluates the egg picking behavior when there is no assisting device and there are interference items;

[0014] The timing module and the counting module are separately set inside the three difficulty displays. The timing module inside any difficulty display has standard times for different stages according to the egg picking stage. Among them, the standard time set inside the assisted operation state is the shortest, the standard time set inside the interference operation state is the longest, and the standard time set inside the non-assisted operation state is between the two;

[0015] Inside the central control module, each difficulty display has a standard egg searching time for the egg searching stage, a standard egg picking time for the egg picking stage, and a standard transferring time for the transferring stage, which include

[0016] The assisted operation state stores a first-level standard egg searching time, a first-level standard egg picking time, and a first-level standard transferring time;

[0017] The non-assisted operation state stores a second-level standard egg searching time, a second-level standard egg picking time, and a second-level standard transferring time;

[0018] The interference operation state stores three - level standard egg - searching times, three - level standard picking times, and three - level standard transfer times.

[0019] Further, in the egg - picking stage, the central control module stores a timing module displayed according to each difficulty level, and times the three stages of the egg - picking stage respectively;

[0020] The central control module stores a behavior pointer for detecting the egg - picking operation, which captures the start action of the egg - picking behavior. When the egg - picking behavior in the fixed mode is captured by the behavior pointer of the egg - picking operation, the central control module controls the timing module in this fixed mode to start timing and enters the egg - searching stage;

[0021] At the start of the egg - searching stage, the image collector collects images inside the egg - picking dish in real - time. When it detects that the egg - picking part touches the cumulus - oocyte complex imitation, when the timing module ends, the time used is recorded as the first duration, and then it enters the picking stage;

[0022] At the start of the picking stage, the timing module is cleared and starts timing again. When the image collector collects an image of the egg - picking part carrying the cumulus - oocyte complex imitation and leaving the follicular fluid, the timing module ends timing, and the time used is recorded as the second duration, and then it enters the transfer stage;

[0023] At the start of the transfer stage, the timing module is cleared and starts timing again. When the image collector collects an image of the cumulus - oocyte complex imitation leaving the egg - picking part and entering the double - well dish, the timing module ends timing, and the time used is recorded as the third duration. After the central control module saves and records this detection of the egg - picking operation record, it is cleared again, and this egg - picking operation is completed. When the behavior pointer of the egg - picking operation captures the start action again, it enters a new egg - picking operation process record.

[0024] Further, an indicator light alarm connected to the central control module is set on the microscope. When the cumulus - oocyte complex imitation drops during the transfer stage and the entry of the cumulus - oocyte complex imitation into the double - well dish is not detected, the central control module determines that the operation is unqualified, saves and records this record, then controls the timing module to be cleared, controls the indicator light alarm to flash and give an alarm to prompt the training personnel.

[0025] Further, in the central control module, for any single complete egg - picking stage, according to the standard times in any difficulty display, including the standard egg - searching time, the standard picking time, and the standard transfer time, compare them with the recorded first duration, second duration, and third duration one by one to determine whether any stage in this egg - picking stage is qualified;

[0026] For any single complete egg - picking stage,

[0027] If the first duration is less than or equal to the standard egg retrieval time, the central control module determines that the current egg retrieval stage is qualified, and controls the counting module to count the number of qualified times in the egg retrieval stage;

[0028] If the first duration is greater than the standard egg retrieval time, the central control module determines that the current egg retrieval stage is unqualified;

[0029] If the second duration is less than or equal to the standard picking time, the central control module determines that the current picking stage is qualified, and controls the counting module to count the number of qualified times in the picking stage;

[0030] If the second duration is greater than the standard picking time, the central control module determines that the current picking stage is unqualified;

[0031] If the third duration is less than or equal to the standard transfer time, the central control module determines that the current transfer stage is qualified, and controls the counting module to count the number of qualified times in the transfer stage;

[0032] If the third duration is greater than the standard transfer time, the central control module determines that the current transfer stage is unqualified.

[0033] Further, for any complete egg retrieval stage, according to the recorded first duration, second duration, and third duration, the total duration of the current egg retrieval operation is finally calculated and summarized. In the central control module, there is a standard total time set according to the standard time of each stage in the egg retrieval stage. By comparing the two, it is determined whether the current egg retrieval operation is qualified;

[0034] If the total duration is less than or equal to the standard total time of the egg retrieval stage, the central control module determines that the total duration of the current egg retrieval operation is qualified, records the duration of the current egg retrieval operation, and makes a qualified judgment on the total duration of the next egg retrieval operation;

[0035] If the total duration is greater than the standard total time of the egg retrieval stage, the central control module determines that the current egg retrieval operation is unqualified, records the duration of the current egg retrieval operation, and makes a qualified judgment on the total duration of the next egg retrieval operation;

[0036] Until the duration of the egg retrieval operation is completely judged, the qualified operation judgment is stopped.

[0037] Further, for the egg retrieval stages operated by the same training personnel multiple times, for any complete egg retrieval stage among them, according to its different difficulty display states, single - time scoring is carried out one by one. According to the average value of the overall egg retrieval duration and the number of unqualified times in the overall egg retrieval stage, the overall score is calculated. In the central control module, there are qualified scores and good scores for the overall egg retrieval stage, and the good score is greater than the qualified score;

[0038] There is a single - time difficulty coefficient stored in the central control module when displaying various difficulties, including an auxiliary difficulty coefficient for the auxiliary operation state, a non - auxiliary difficulty coefficient for the non - auxiliary operation state, and an interference difficulty coefficient for the interference operation state. The auxiliary difficulty coefficient is the smallest, the interference difficulty coefficient is the largest, the non - auxiliary difficulty coefficient is greater than the auxiliary difficulty coefficient and less than the interference difficulty coefficient;

[0039] If the overall score is less than the passing score, it is determined that the training result of the trainee is unqualified;

[0040] If the overall score is equal to the passing score, it is determined that the training result of the trainee is qualified;

[0041] If the overall score is greater than the passing score and less than or equal to the good score, it is determined that the training result of the trainee is good;

[0042] If the overall score is greater than the good score, it is determined that the training result of the trainee is excellent.

[0043] Furthermore, for the egg - picking stage of a trainee's multiple operations, according to the number of qualified times in each stage, calculate the actual egg - searching qualified ratio, actual egg - picking qualified ratio, and actual transfer qualified ratio. There are standard egg - picking stage qualified ratios in the central control module, including a standard egg - searching stage qualified ratio, a standard egg - picking stage qualified ratio, and a standard transfer stage qualified ratio;

[0044] When the actual egg - searching stage qualified ratio, actual egg - picking stage qualified ratio, and actual transfer stage qualified ratio are respectively greater than or equal to their corresponding standard egg - picking stage qualified ratios, start to judge the egg - picking stability rate of the egg - picking stage;

[0045] When any one of the actual egg - searching stage qualified ratio, actual egg - picking stage qualified ratio, and actual transfer stage qualified ratio is less than its corresponding standard egg - picking stage qualified ratio, the central control module marks the corresponding item of this trainee as a key training item and displays it on the third eyepiece.

[0046] Furthermore, for the egg - picking stage of a trainee's multiple operations, according to the different difficulty display states and the recorded first duration, second duration, and third duration, calculate the egg - picking stability rate of the egg - picking stage and judge the stability degree of the trainee's egg - picking stage. In the central control module, there is a first standard egg - picking stability rate during auxiliary operation, a second standard egg - picking stability rate during non - auxiliary operation, and a third standard egg - picking stability rate during interference operation. The first standard egg - picking stability rate is the smallest, the third standard egg - picking stability rate is the largest, the second standard egg - picking stability rate is greater than the first standard egg - picking stability rate and less than the third standard egg - picking stability rate;

[0047] For the egg - picking stage of a trainee's multiple operations,

[0048] In the auxiliary operation, if the egg-picking stability rate is greater than or equal to the first standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is stable; if the egg-picking stability rate is less than the first standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is unstable.

[0049] In the non-auxiliary operation, if the egg-picking stability rate is greater than or equal to the second standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is stable; if the egg-picking stability rate is less than the second standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is unstable.

[0050] In the interference operation, if the egg-picking stability rate is greater than or equal to the third standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is stable; if the egg-picking stability rate is less than the third standard egg-picking stability rate, the central control module determines that the egg-picking operation of the trainee is unstable.

[0051] Furthermore, the central control module can arrange the actual total egg-picking time of all trainees from largest to smallest; when the actual total egg-picking time of the trainees is equal, the central control module arranges the trainees from largest to smallest according to the egg-picking stability rate in their picking stages, and displays the final arrangement order on the third eyepiece.

[0052] Compared with the prior art, the beneficial effects of the present invention are as follows: by installing an image collector on the microscope, the image collector mainly monitors and collects the images in the egg-picking dish, double-well dish, inside the egg-picking dish and inside the double-well dish in real time, and feeds the image signals back to the central control module. The central control module analyzes the images in the egg-picking dish, double-well dish, inside the egg-picking dish and inside the double-well dish, divides the egg-picking stage into an egg-searching stage, a picking stage and a transferring stage, and counts through the control of the timing module to accurately reflect the time used by the trainee in the egg-searching stage, picking stage and transferring stage. Finally, the central control module analyzes the time on the timing module, accurately feedbacks the operation conditions of the trainee in the egg-searching stage, picking stage and transferring stage, and displays the results on the third eyepiece, which is convenient for the trainee to find the parts where they make mistakes and the parts where they perform well, take their deficiencies as the key training parts, and learn the corresponding medical knowledge and skills; for reproductive centers with small to medium-sized diagnosis and treatment scales, it provides good learning and practice conditions for new personnel and ensures the cultivation of talents; for reproductive centers with large diagnosis and treatment scales, it greatly reduces the teaching pressure of the personnel with proficient egg-picking operations in the embryo laboratory and improves the work efficiency of the personnel with proficient egg-picking operations.

[0053] Furthermore, the present invention sets three difficulty display states in the egg picking training device, refines the training of trainees according to the relevant difficulty, ensures that the trainees present a step-by-step process in the egg picking operation training, and is conducive to the trainees gradually mastering the egg picking operation. At the same time, it ensures that trainees with different proficiency can operate the device, broadens the applicability of the device, sets different standard times for different difficulty levels, and further refines the standards for the egg picking stage to ensure that subsequent training scores have sufficient basis.

[0054] Furthermore, when the egg-picking piece operated by the trainee touches the replica of the egg-coronary-cumulus complex, it indicates that the trainee has found the replica of the egg-coronary-cumulus complex. The egg-picking piece touching the replica of the egg-coronary-cumulus complex is used as a signal to make the central control module determine that the egg-searching stage is over. When the replica of the egg-coronary-cumulus complex is touched by the egg-picking piece but not picked up by the egg-picking piece, the central control module is likely to determine that the egg-picking piece has carried the replica of the egg-coronary-cumulus complex. In order to avoid the above situation, the egg-picking piece carrying the replica of the egg-coronary-cumulus complex and leaving the follicular fluid is used as a signal. The central control module determines that the picking stage is over; when the egg crown-cumulus complex replica enters the double-well dish but the egg picking piece does not unload the egg crown-cumulus complex replica into the double-well dish, the central control module is likely to determine that the egg crown-cumulus complex replica has entered the double-well dish. To avoid the above situation, the central control module determines that the transfer stage is over when it is detected that the egg crown-cumulus complex replica leaves the egg picking piece and enters the double-well dish; after the transfer stage is over, the timing module is reset to zero, and when the start action is captured again, a new egg picking operation process record is re-entered.

[0055] Furthermore, the present invention directly determines that the egg coronal cumulus complex replica falls during the transfer stage and the egg coronal cumulus complex replica does not enter the double-well dish, thereby preventing problems from occurring in the subsequent determination process.

[0056] Furthermore, the present invention determines the degree of qualification for each stage of the egg picking stage, ensuring that there is a basis for detailed judgment of the egg picking stage, while paving the way for specific scoring of a single egg picking stage. At the same time, unqualified egg picking stages will be recorded to facilitate subsequent detailed judgment.

[0057] Furthermore, the present invention determines the overall egg picking stage for any complete egg picking stage, controls whether the trained personnel are qualified in the entire egg picking process, determines whether the overall operation is qualified, and proceeds to determine the total duration of the next complete operation.

[0058] Furthermore, the present invention calculates the scores of multiple egg retrieval operations performed by the same trainee at any difficulty level, determines the score of the trainee at this difficulty level, thereby calculates the performance of the trainee in egg retrieval training, and thus divides the degree of mastery of the trainee in egg retrieval operations.

[0059] Furthermore, the present invention calculates the qualification rate for each stage of the trainee's egg retrieval stage, determines the qualified proportion of each stage of the trainee, analyzes the operation process of the trainee, and marks the key items that the trainee needs to improve.

[0060] Furthermore, on the premise of ensuring the qualification of the egg retrieval stage, the more times the trainee passes the egg retrieval stage, the more proficient the operation is. The present invention calculates the smooth rate of the trainee's egg retrieval stage, aiming to distinguish the operation conditions of the trainees after passing the qualification, so that the trainees after passing the qualification can improve their operation smooth rate and ensure smooth and stable egg retrieval operations.

[0061] Furthermore, the present invention determines the actual total time of the trainee's egg retrieval by the central control module and ranks them. When the actual total time of egg retrieval is the same, it is further divided according to the smooth rate of egg retrieval in the egg retrieval stage. The higher the smooth rate of egg retrieval in the egg retrieval stage, the higher the ranking. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 It is a side view in the structural schematic diagram of the egg retrieval training device based on the oocyte-cumulus complex mimic of the embodiment;

[0063] Figure 2 It is a front view in the structural schematic diagram of the egg retrieval training device based on the oocyte-cumulus complex mimic of the embodiment;

[0064] In the figure, microscope 1, double-well dish 2, egg retrieval dish 3, third eyepiece 4, indicator light alarm 5. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0065] In order to make the objectives and advantages of the present invention clearer and more understandable, the present invention will be further described below in conjunction with the embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0066] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the present invention and do not limit the protection scope of the present invention.

[0067] It should be noted that in the description of the present invention, the terms indicating the direction or positional relationship such as "upper", "lower", "left", "right", "inner", "outer", etc. are based on the direction or positional relationship shown in the drawings. This is only for convenience of description and does not indicate or imply that the device or component must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention.

[0068] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and defined, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0069] Please refer to Figure 1 、 Figure 2 as shown in Figure 1 the side view in the structural schematic diagram of the egg retrieval training device based on the cumulus-oocyte complex mimetic described in the embodiment, Figure 2 and the front view in the structural schematic diagram of the egg retrieval training device based on the cumulus-oocyte complex mimetic described in the embodiment.

[0070] The present invention provides an egg retrieval training device based on a cumulus-oocyte complex mimetic, including

[0071] a microscope 1, a double-well dish 2, and an egg retrieval dish 3. A third eyepiece 4 is provided on the microscope 1, and the third eyepiece 4 is used to display image data;

[0072] An image collector is arranged inside the microscope 1 and is used to capture the internal images of the egg retrieval dish 3 and the double-well dish 2;

[0073] A central control module, a timing module, and a counting module are arranged inside the microscope 1. The central control module is connected to the timing module, the counting module, the image collector, and the third eyepiece 4. The central control module divides the egg retrieval stage into an egg searching stage, an egg picking stage, and a transfer stage, and judges the egg retrieval behavior evaluation according to different difficulties after judging the egg retrieval difficulty. By receiving the image signal transmitted by the image collector, it analyzes the egg retrieval operation, controls the timing module to time, times the egg searching stage, the egg picking stage, and the transfer stage, controls the counting module to count the qualified times in the egg searching stage, the egg picking stage, and the transfer stage, analyzes the completion time of each stage, the total completion time, and the stability to determine whether the training personnel are qualified, ranks each training personnel, and displays the timing data of the timing module, the counting data of the counting module, the result of the performance judgment, and the ranking of the training personnel on the third eyepiece 4;

[0074] The microscope 1 is a stereomicroscope 1. This microscope 1 has no stage and is fixedly installed inside the laminar flow bench. Both the double-well dish 2 and the egg-picking dish 3 are arranged on the workbench surface. The operator of the entire stereomicroscope 1 uses binoculars. There is a third eyepiece 4 beside the binoculars of this stereomicroscope 1, which is used to project the interface observed by the operator to assist the egg-picking personnel in watching the operation. The image collector, timing module, and central control module are all located inside the microscope 1. To prevent chaos in the figure, they are not drawn in the figure; the egg-picking tool is a Pasteur pipette made of glass. The distance between the egg-picking dish 3 and the double-well dish 2 is about 20 cm. To avoid knocking down the double-well dish 2 during the egg-picking operation, the distance between the double-well dish 2 and the egg-picking personnel should be greater than the distance between the egg-picking dish 3 and the egg-picking personnel; the specific egg-picking operation is as follows: First, find the target object in the egg-picking dish 3. After finding it, control the suction by squeezing the rubber ball to suck the target object into the egg-picking tool. Then, keep the suction unchanged. Use the left hand to hold the double-well dish 2 placed horizontally side by side with the egg-picking dish 3 under the lens. The egg-picking dish 3 is pushed aside by the double-well dish 2. Then, spit the target object in the egg-picking dish 3 into the double-well dish 2. Finally, the double-well dish 2 returns to its original position, and the egg-picking dish 3 also returns to its original position under the lens to continue looking for the target object; this egg-picking operation is relatively conventional, so the egg-picking operation is not repeated and explained below.

[0075] In the present invention, by installing an image collector on the microscope, the image collector mainly monitors and collects the images of the egg-picking dish, double-well dish, inside the egg-picking dish, and inside the double-well dish in real time, and feeds the image signals back to the central control module. The central control module divides the egg-picking stage into an egg-searching stage, a picking stage, and a transferring stage by analyzing the images of the egg-picking dish, double-well dish, inside the egg-picking dish, and inside the double-well dish, and counts by controlling the timing module to accurately reflect the time used by the training personnel in the egg-searching stage, picking stage, and transferring stage. Finally, the central control module analyzes the time on the timing module and accurately feeds back the operation conditions of the training personnel in the egg-searching stage, picking stage, and transferring stage, and displays the results on the third eyepiece, which is convenient for the training personnel to find the parts where they make mistakes and the parts where they perform well, and take their deficiencies as the key training parts and learn the corresponding medical knowledge and skills; for reproductive centers with small to medium-sized diagnosis and treatment scales, it provides good learning and practice conditions for new personnel and ensures the cultivation of talents; for reproductive centers with large diagnosis and treatment scales, it greatly reduces the teaching pressure of the personnel with proficient egg-picking operations in the embryo laboratory and improves the work efficiency of the personnel with proficient egg-picking operations.

[0076] Specifically, in this embodiment, the central control module in the microscope stores three difficulty display states, which respectively include, according to the increasing difficulty level,

[0077] The first difficulty is the assisted operation state, which is connected to the egg-picking operation assistance device and evaluates the egg-picking behavior when there is an assistance device for the egg-picking operation.

[0078] The second difficulty level is the no-assistance operation state, which evaluates the egg retrieval behavior without an assistance device;

[0079] The third difficulty level is the interference operation state, which evaluates the egg retrieval behavior without an assistance device and with interference items;

[0080] A timing module and the counting module are separately set in each of the three difficulty level displays. The timing module in any difficulty level display has standard times for different stages according to the egg retrieval stage. Among them, the standard time set in the assistance operation state is the shortest, the standard time set in the interference operation state is the longest, and the standard time set in the no-assistance operation state is between the two;

[0081] In the central control module, each difficulty level display has a standard egg searching time for the egg searching stage, a standard picking time for the picking stage, and a standard transferring time for the transferring stage, which includes,

[0082] The assistance operation state stores a first-level standard egg searching time, a first-level standard picking time, and a first-level standard transferring time;

[0083] The no-assistance operation state stores a second-level standard egg searching time, a second-level standard picking time, and a second-level standard transferring time;

[0084] The interference operation state stores a third-level standard egg searching time, a third-level standard picking time, and a third-level standard transferring time.

[0085] In the assistance operation state, the egg retrieval training device is connected to the egg retrieval operation assistance device, and the training personnel perform egg retrieval operations through the egg retrieval operation assistance device. Only egg-cumulus-oocyte complexes replicas are stored in the egg retrieval dish.

[0086] In the no-assistance operation state, the egg retrieval training device has no assistance device, and the training personnel perform egg retrieval operations by operating the egg retrieval training device. Only egg-cumulus-oocyte complexes replicas are stored in the egg retrieval dish.

[0087] In the interference operation state, the egg retrieval training device has no assistance device, and the training personnel perform egg retrieval operations by operating the egg retrieval training device. Egg-cumulus-oocyte complexes replicas and interference items such as blood are stored in the egg retrieval dish.

[0088] The present invention sets three difficulty level display states in the egg retrieval training device, refines the training of the training personnel according to the relevant difficulties, ensures that the training of the training personnel in the egg retrieval operation presents a gradual process, is conducive to the training personnel gradually mastering the egg retrieval operation. At the same time, it ensures that training personnel with different proficiency levels can operate this device, broadens the applicability of this device, sets different standard times for different difficulty levels, further standardizes and refines the egg retrieval stage, and ensures that there is a sufficient basis for subsequent training scoring.

[0089] Specifically, in this embodiment, during the egg retrieval stage, the central control module stores a timing module that displays according to each difficulty level, and times the three stages of the egg retrieval stage respectively;

[0090] The central control module stores a behavior pointer for detecting the egg retrieval operation, which captures the start action of the egg retrieval behavior. When the egg retrieval behavior in the fixed mode is captured by the behavior pointer of the egg retrieval operation, the central control module controls the timing module in this fixed mode to start timing and enters the egg searching stage;

[0091] At the beginning of the egg searching stage, the image collector collects the image inside the egg retrieval dish in real time. When it is detected that the egg retrieval part touches the cumulus-oocyte complex imitation, at the end of the timing module, the time used is recorded as the first duration, and then it enters the egg picking stage;

[0092] At the beginning of the egg picking stage, the timing module is cleared and starts timing again. When the image collector collects the image of the egg retrieval part carrying the cumulus-oocyte complex imitation and leaving the follicular fluid, the timing module ends timing, and the time used is recorded as the second duration, and then it enters the transfer stage;

[0093] At the beginning of the transfer stage, the timing module is cleared and starts timing again. When the image collector collects the image of the cumulus-oocyte complex imitation leaving the egg retrieval part and entering the double-well dish, the timing module ends timing, and the time used is recorded as the third duration. After the central control module saves and records this detected egg retrieval operation record, it is cleared again, and this egg retrieval operation is completed. When the behavior pointer of the egg retrieval operation captures the start action again, it re-enters the record of a new egg retrieval operation process.

[0094] When the egg retrieval part operated by the trainer touches the cumulus-oocyte complex imitation, it means that the trainer has found the cumulus-oocyte complex imitation. Taking the contact of the egg retrieval part with the cumulus-oocyte complex imitation as a signal, the central control module determines that the egg searching stage ends; when the cumulus-oocyte complex imitation is touched by the egg retrieval part but not picked up by the egg retrieval part, the central control module is likely to determine that the egg retrieval part has carried the cumulus-oocyte complex imitation. To avoid the above situation, taking the egg retrieval part carrying the cumulus-oocyte complex imitation and leaving the follicular fluid as a signal, the central control module determines that the egg picking stage ends; when the cumulus-oocyte complex imitation enters the double-well dish 2 but the egg retrieval part does not unload the cumulus-oocyte complex imitation into the double-well dish 2, the central control module is likely to determine that the cumulus-oocyte complex imitation has entered the double-well dish 2. To avoid the above situation, taking the detection of the cumulus-oocyte complex imitation leaving the egg retrieval part and entering the double-well dish 2 as a signal, the central control module determines that the transfer stage ends; after the transfer stage ends, the timing module then returns to zero, and when the start action is captured again, it re-enters the record of a new egg retrieval operation process.

[0095] Specifically, in this embodiment, an indicator alarm 5 connected to the central control module is provided on the microscope. When the oocyte-cumulus complex mimic drops during the transfer stage and the oocyte-cumulus complex mimic is not detected to enter the double-well dish, the central control module determines that the operation is unqualified, saves and records this record, then controls the timing module to reset, controls the indicator alarm to flash and give an alarm, and gives a prompt to the training personnel.

[0096] In the present invention, when the oocyte-cumulus complex mimic drops during the transfer stage and the oocyte-cumulus complex mimic is not detected to enter the double-well dish, the operation is directly determined to be unqualified, preventing problems from occurring in the subsequent determination process.

[0097] Specifically, in this embodiment, for any single complete egg retrieval stage, according to the standard times in any difficulty display, including the standard egg searching time, the standard picking time, and the standard transfer time, compare them with the recorded first duration, second duration, and third duration one by one to determine whether any stage in this egg retrieval stage is qualified;

[0098] For any single complete egg retrieval stage,

[0099] If the first duration is less than or equal to the standard egg searching time, the central control module determines that this egg searching stage is qualified and controls the counting module to count the number of qualified egg searching stages;

[0100] If the first duration is greater than the standard egg searching time, the central control module determines that this egg searching stage is unqualified;

[0101] If the second duration is less than or equal to the standard picking time, the central control module determines that this picking stage is qualified and controls the counting module to count the number of qualified picking stages;

[0102] If the second duration is greater than the standard picking time, the central control module determines that this picking stage is unqualified;

[0103] If the third duration is less than or equal to the standard transfer time, the central control module determines that this transfer stage is qualified and controls the counting module to count the number of qualified transfer stages;

[0104] If the third duration is greater than the standard transfer time, the central control module determines that this transfer stage is unqualified.

[0105] In this embodiment, in the assisted operation state, there are a first-level standard egg searching time X1, a first-level standard picking time J1, and a first-level standard transfer time Z1; in the non-assisted operation state, there are a second-level standard egg searching time X2, a second-level standard picking time J2, and a second-level standard transfer time Z2; in the interference operation state, there are a third-level standard egg searching time X3, a third-level standard picking time J3, and a third-level standard transfer time Z3.

[0106] If the trainer operates in the assisted operation mode, where the first-level standard egg search time X1 = 2s, the first-level standard picking time J1 = 2s, and the first-level standard transfer time Z1 = 3s.

[0107] In the embodiment, the first duration of the trainer is denoted as Xt1, the second duration is denoted as Jt2, and the third duration is denoted as Zt3.

[0108] If the first duration is Xt1 = 1s, the central control module determines that the current egg search stage is qualified and controls the counting module to count the number of qualified times in the egg search stage; if the first duration is Xt1 = 3s, the central control module determines that the current egg search stage is unqualified.

[0109] If the second duration is Jt2 = 1s, the central control module determines that the current picking stage is qualified and controls the counting module to count the number of qualified times in the picking stage; if the second duration is Jt2 = 4s, the central control module determines that the current picking stage is unqualified.

[0110] If the third duration is Zt3 = 2s, the central control module determines that the current transfer stage is qualified and controls the counting module to count the number of qualified times in the transfer stage; if the third duration is Zt3 = 4s, the central control module determines that the current transfer stage is unqualified. The judgment process for each egg picking stage in other modes is the same as the above process, and this specification will not elaborate further.

[0111] In the present invention, the qualification degree of each stage in the egg picking stage is determined to ensure that there is a basis for the detailed judgment of the egg picking stage. At the same time, it lays the foundation for the specific scoring of a single egg picking stage, and at the same time, the unqualified egg picking stages will be recorded for subsequent refined judgment.

[0112] Specifically, in this embodiment, for any complete egg picking stage, according to the recorded first duration, second duration, and third duration, the total duration of the current egg picking operation is finally calculated and summarized. There is a standard total time set according to the standard time of each stage in the egg picking stage in the central control module. By comparing the magnitudes of the two, it is determined whether the current egg picking operation is qualified;

[0113] If the total duration is less than or equal to the standard total time of the egg picking stage, the central control module determines that the total duration of the current egg picking operation is qualified, records the duration of the current egg picking operation, and makes a qualification judgment on the total duration of the next egg picking operation;

[0114] If the total duration is greater than the standard total time of the egg picking stage, the central control module determines that the current egg picking operation is unqualified, records the duration of the current egg picking operation, and makes a qualification judgment on the total duration of the next egg picking operation;

[0115] Until the determination of the egg retrieval operation duration is completely finished, stop the determination of the qualified operation.

[0116] If the trainer operates in the assisted operation state, the total duration T of this egg retrieval operation 总 :

[0117] T i = Xt1 + Jt2 + Zt3;

[0118] Wherein, T i is the total duration of the trainer performing the egg retrieval operation in the assisted operation state in this embodiment, where i is the trainer serial number, i = 1, 2,..., n;

[0119] Xt1 is the first duration of the trainer in this embodiment;

[0120] Jt2 is the second duration of the trainer in this embodiment;

[0121] Zt3 is the third duration of the trainer in this embodiment.

[0122] In this embodiment, in the assisted operation state, where the first-level standard egg search time X1 = 2s, the first-level standard picking time J1 = 2s, the first-level standard transfer time Z1 = 3s, and the standard total time T0 of the picking stage = X1 + J1 + Z1 = 2 + 2 + 3 = 7s.

[0123] If T i = 5s, the central control module determines that the total duration of this egg retrieval operation is qualified, records the duration of this egg retrieval operation, and makes a qualified determination for the total duration of the next egg retrieval operation;

[0124] If T i = 8s, the central control module determines that this egg retrieval operation is unqualified, records the duration of this egg retrieval operation, and makes a qualified determination for the total duration of the next egg retrieval operation;

[0125] Until the determination of the egg retrieval operation duration is completely finished, stop the determination of the qualified operation.

[0126] The present invention determines the overall egg retrieval stage for any complete egg retrieval stage, overall controls whether there is a qualified situation for the trainer during the entire egg retrieval process, determines whether the overall operation is qualified, and continues to make a determination for the total duration of the next complete operation.

[0127] Specifically, in this embodiment, for the egg retrieval stage where the same trainee operates multiple times, for any one complete egg retrieval stage among them, according to the different difficulty display states it is in, a single score is given one by one. Based on the average value of the overall egg retrieval duration and the number of unqualified times in the overall egg retrieval stage, the overall score is calculated. In the central control module, there are qualified scores and good scores for the overall egg retrieval stage, and the good score is greater than the qualified score;

[0128] In the central control module, there is a single - time difficulty coefficient when displaying each difficulty, including an auxiliary difficulty coefficient for the assisted operation state, a non - assisted difficulty coefficient for the non - assisted operation state, and an interference difficulty coefficient for the interference operation state. The auxiliary difficulty coefficient is the smallest, the interference difficulty coefficient is the largest, the non - assisted difficulty coefficient is greater than the auxiliary difficulty coefficient and less than the interference difficulty coefficient;

[0129] If the overall score is less than the qualified score, it is determined that the training result of the trainee is unqualified;

[0130] If the overall score is equal to the qualified score, it is determined that the training result of the trainee is qualified;

[0131] If the overall score is greater than the qualified score and less than or equal to the good score, it is determined that the training result of the trainee is good;

[0132] If the overall score is greater than the good score, it is determined that the training result of the trainee is excellent.

[0133] In the central control module, there is a single - time difficulty coefficient when displaying each difficulty, including an auxiliary difficulty coefficient a for the assisted operation state, a non - assisted difficulty coefficient b for the non - assisted operation state, and an interference difficulty coefficient c for the interference operation state. The auxiliary difficulty coefficient a is less than the non - assisted difficulty coefficient b, and the interference difficulty coefficient c is greater than the auxiliary difficulty coefficient a and greater than the non - assisted difficulty coefficient b.

[0134] For the same trainee, in the egg retrieval stage with multiple operations in the assisted operation state, the number of operations is m.

[0135]

[0136] Among them, is the average value of the overall egg retrieval duration of the trainee with trainee number i in the assisted operation state, i is the trainee number, i = 1, 2,..., n;

[0137] T j is the overall operation duration of the trainee with trainee number i when the operation number is j in the assisted operation state for overall egg retrieval, j = 1, 2,..., m;

[0138] m is the total number of operations.

[0139] Overall score Pi:

[0140] In the central control module, there is a qualified score P for the overall egg retrieval stage 合格 , a good score P 良好 , and the good score P 良好 is greater than the qualified score P 合格 ;

[0141] If the overall score Pi is less than the qualified score P 合格 , it is determined that the training result of the trainee is unqualified;

[0142] If the overall score Pi is equal to the qualified score P 合格 , it is determined that the training result of the trainee is qualified;

[0143] If the overall score Pi is greater than the qualified score P 合格 , and less than or equal to the good score P 良好 , it is determined that the training result of the trainee is good;

[0144] If the overall score Pi is greater than the good score P 良好 , it is determined that the training result of the trainee is excellent.

[0145] The present invention calculates the scores of multiple egg retrieval operations performed by the same trainee at any difficulty level, determines the score of the trainee at this difficulty level, thereby calculates the training result of the trainee's egg retrieval training, and thus divides the mastery level of the trainee for the egg retrieval stage.

[0146] Specifically, in this embodiment, for the egg retrieval stage of multiple operations of the same trainee, according to the number of qualified times in each stage, the actual egg searching qualified ratio, the actual egg picking qualified ratio, and the actual transfer qualified ratio are calculated. In the central control module, there is a standard egg retrieval stage qualified ratio, including the standard egg searching stage qualified ratio, the standard egg picking stage qualified ratio, and the standard transfer stage qualified ratio;

[0147] When the actual egg searching qualified ratio, the actual egg picking qualified ratio, and the actual transfer qualified ratio are respectively greater than or equal to their corresponding standard egg picking stage qualified ratios, start to judge the egg retrieval stability rate of the egg retrieval stage;

[0148] When any one of the actual egg searching qualified ratio, the actual egg picking qualified ratio, and the actual transfer qualified ratio is less than its corresponding standard egg retrieval stage qualified ratio, the central control module marks the item corresponding to this trainee as a key training item and displays it on the third eyepiece.

[0149] For the trainer with the trainer number \(i = 1\), among the total number of 10 operations, there are 5 qualified times in actual egg retrieval, 6 qualified times in egg picking, and 8 qualified times in actual transfer.

[0150] The qualified ratio in the actual egg retrieval stage is 0.5, the qualified ratio in the actual egg picking stage is 0.6, and the qualified ratio in the actual transfer stage is 0.8. In the central control module, there is a standard qualified ratio in the egg picking stage, including a standard qualified ratio of 0.7 in the standard egg retrieval stage, a standard ratio of 0.7 in the standard egg picking stage, and a standard qualified ratio of 0.7 in the standard transfer stage.

[0151] For the trainer with the trainer number \(i = 1\), the central control module marks the actual egg retrieval stage and the actual egg picking stage of this trainer as key training items and displays them on the third eyepiece.

[0152] For the trainer with the trainer number \(i = 2\), among the total number of 10 operations, there are 8 qualified times in actual egg retrieval, 9 qualified times in actual egg picking, and 8 qualified times in actual transfer.

[0153] The qualified ratio in the actual egg retrieval stage is 0.8, the qualified ratio in the actual egg picking stage is 0.9, and the qualified ratio in the actual picking stage is 0.8.

[0154] For the trainer with the trainer number \(i = 2\), start to judge the egg picking stability rate in the egg picking stage.

[0155] The present invention calculates the qualified rate for each stage of the trainer's egg picking stage, judges the qualified ratio of each stage of the trainer, analyzes the operation process of the trainer, and marks the key items that the trainer needs to improve.

[0156] Specifically, in this embodiment, for the egg picking stage of the same trainer's multiple operations, according to the different difficulty display states and the recorded first duration, second duration, and third duration, calculate the egg picking stability rate of the egg picking stage to judge the stability degree of the trainer's egg picking stage; in the central control module, there is a first standard egg picking stability rate during assisted operation, a second standard egg picking stability rate during non-assisted operation, and a third standard egg picking stability rate during interference operation. The first standard egg picking stability rate is the smallest, the third standard egg picking stability rate is the largest, the second standard egg picking stability rate is greater than the first standard egg picking stability rate and less than the third standard egg picking stability rate;

[0157] For the egg picking stage of the same trainer's multiple operations,

[0158] During assisted operation, if the egg picking stability rate is greater than or equal to the first standard egg picking stability rate, the central control module determines that the trainer's egg picking operation is stable; if the egg picking stability rate is less than the first standard egg picking stability rate, the central control module determines that the trainer's egg picking operation is unstable;

[0159] In the non - assisted operation, if the egg - picking smoothness rate is greater than or equal to the second standard egg - picking smoothness rate, the central control module determines that the egg - picking operation of the trainee is stable; if the egg - picking smoothness rate is less than the second standard egg - picking smoothness rate, the central control module determines that the egg - picking operation of the trainee is unstable.

[0160] In the interference operation, if the egg - picking smoothness rate is greater than or equal to the third standard egg - picking smoothness rate, the central control module determines that the egg - picking operation of the trainee is stable; if the egg - picking smoothness rate is less than the third standard egg - picking smoothness rate, the central control module determines that the egg - picking operation of the trainee is unstable.

[0161] In the central control module, the first standard egg - picking smoothness rate d1 in the assisted operation, the second standard egg - picking smoothness rate d2 in the non - assisted operation, and the third standard egg - picking smoothness rate d3 in the interference operation. The first standard egg - picking smoothness rate d1 is the smallest, the third standard egg - picking smoothness rate d3 is the largest, the second standard egg - picking smoothness rate d2 is greater than the first standard egg - picking smoothness rate d1 and less than the third standard egg - picking smoothness rate d3.

[0162] Egg - picking smoothness rate:

[0163]

[0164] Wherein, Wi is the egg - picking smoothness rate of the trainee's operation in the assisted operation state of this embodiment;

[0165] Xt1 is the first duration of the trainee in this embodiment;

[0166] Jt2 is the second duration of the trainee in this embodiment;

[0167] Zt3 is the third duration of the trainee in this embodiment.

[0168] X1 is the first - level standard egg - searching time in the assisted operation;

[0169] J1 is the first - level standard egg - picking time in the assisted operation;

[0170] Z1 is the first - level standard transfer time in the assisted operation;

[0171] T0 is the standard total time of the egg - picking stage in the assisted operation.

[0172] For the egg - picking stage of multiple operations by the same trainee,

[0173] In the assisted operation, if the egg - picking smoothness rate Wi is greater than or equal to the first standard egg - picking smoothness rate d1, the central control module determines that the egg - picking operation of the trainee is stable; if the egg - picking smoothness rate Wi is less than the first standard egg - picking smoothness rate d1, the central control module determines that the egg - picking operation of the trainee is unstable.

[0174] On the premise of ensuring the qualification in the egg retrieval stage, the more times a training person is qualified in the egg retrieval stage, the more proficient the operation is. Calculate the stability rate of the training person in the egg retrieval stage, aiming to distinguish the operation conditions of the training person after qualification, so that the training person after qualification can improve their own operation stability rate and ensure smooth and stable egg retrieval operation.

[0175] Specifically, in this embodiment, the central control module can arrange the actual total egg retrieval time of all training persons from largest to smallest; when the actual total egg retrieval time of the training person is equal, the central control module arranges the training persons from largest to smallest according to the egg retrieval stability rate in their retrieval stage, and displays the final arrangement order on the third eyepiece.

[0176] The present invention determines the ranking of the training person according to the actual total egg retrieval time judged by the central control module. When the actual total egg retrieval time is the same, it is further divided according to the egg retrieval stability rate in the egg retrieval stage. The higher the egg retrieval stability rate in the egg retrieval stage, the higher the ranking.

[0177] So far, the technical solution of the present invention has been described in combination with the preferred embodiments shown in the drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the protection scope of the present invention.

[0178] The above are only the preferred embodiments of the present invention and are not used to limit the present invention; for those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An egg retrieval training device based on an oocyte-cumulus complex mimic, characterized in that, Including, a microscope, a double-well dish, and an egg-picking dish. A third eyepiece is provided on the microscope, and the third eyepiece is used to display image data; an image collector, which is arranged inside the microscope and is used to capture the internal images of the egg-picking dish and the double-well dish; a central control module, a timing module, and a counting module are arranged inside the microscope. The central control module is connected to the timing module, the counting module, the image collector, and the third eyepiece. The central control module divides the egg-picking stage into an egg-searching stage, an egg-picking stage, and a transfer stage. It evaluates the egg-picking behavior according to different difficulties through the judgment of the egg-picking difficulty. By receiving the image signal transmitted by the image collector, it analyzes the egg-picking operation, controls the timing module to time, times the egg-searching stage, the egg-picking stage, and the transfer stage, controls the counting module to count the qualified times in the egg-searching stage, the egg-picking stage, and the transfer stage, analyzes the completion time of each stage, the total completion time, and the stability to determine whether the trainee is qualified, ranks each trainee, and displays the timing data of the timing module, the counting data of the counting module, the result of performance judgment, and the ranking of the trainee on the third eyepiece.

2. The egg retrieval training device based on the oocyte-cumulus complex mimic according to claim 1, wherein, The central control module inside the microscope stores three difficulty display states, which respectively include, according to the increasing difficulty level, the first difficulty is the assisted operation state, which is connected to the egg-picking operation assistance device and evaluates the egg-picking behavior when there is an assistance device for the egg-picking operation; the second difficulty is the non-assisted operation state, which evaluates the egg-picking behavior without an assistance device; the third difficulty is the interference operation state, which evaluates the egg-picking behavior without an assistance device and with interference items; Separate timing modules and the counting module are set inside the three difficulty displays. The timing module inside any difficulty display has standard times for different stages according to the egg-picking stage. Among them, the standard time set inside the assisted operation state is the shortest, the standard time set inside the interference operation state is the longest, and the standard time set inside the non-assisted operation state is between the two; Inside the central control module, each difficulty display has a standard egg-searching time for the egg-searching stage, a standard egg-picking time for the egg-picking stage, and a standard transfer time for the transfer stage. It includes, the assisted operation state stores a first-level standard egg-searching time, a first-level standard egg-picking time, and a first-level standard transfer time; the non-assisted operation state stores a second-level standard egg-searching time, a second-level standard egg-picking time, and a second-level standard transfer time; the interference operation state stores a third-level standard egg-searching time, a third-level standard egg-picking time, and a third-level standard transfer time.

3. The egg retrieval training device based on the oocyte-cumulus complex mimic according to claim 2, wherein, During the egg-picking stage, the central control module stores timing modules according to each difficulty display and times the three stages of the egg-picking stage respectively; The central control module stores a behavior pointer for detecting the egg-picking operation, which captures the start action of the egg-picking behavior. After the egg-picking behavior in the fixed mode is captured by the behavior pointer of the egg-picking operation, the central control module controls the timing module in this fixed mode to start timing and enters the egg-searching stage; At the beginning of the egg searching stage, the image collector collects the images inside the egg picking dish in real time. When it is detected that the egg picking part touches the cumulus-oocyte complex imitation, at the end of the timing module, the time used is recorded as the first duration, and then the egg picking stage begins; At the beginning of the egg picking stage, the timing module is cleared and starts timing again. When the image collector collects the image of the egg picking part carrying the cumulus-oocyte complex imitation and leaving the follicular fluid, the timing module stops timing, and the time used is recorded as the second duration, and then the transfer stage begins; At the beginning of the transfer stage, the timing module is cleared and starts timing again. When the image collector collects the image of the cumulus-oocyte complex imitation leaving the egg picking part and entering the double-well dish, the timing module stops timing, and the time used is recorded as the third duration. After the central control module saves and records the operation record of this egg picking detection, it is cleared again, and this egg picking operation is completed. When the action pointer of the egg picking operation captures the start action again, a new egg picking operation process record is entered again.

4. The egg retrieval training device based on the oocyte-cumulus complex mimic according to claim 3, wherein An indicator alarm connected to the central control module is set on the microscope. When the cumulus-oocyte complex imitation drops during the transfer stage and the entry of the cumulus-oocyte complex imitation into the double-well dish is not detected, the central control module determines that the operation is unqualified, saves and records this record, then controls the timing module to be cleared, controls the indicator alarm to flash and emit an alarm to prompt the training personnel.

5. The egg retrieval training device based on the cumulus-oocyte complex mimic according to claim 4, characterized in that, Inside the central control module, for any single complete egg picking stage, according to the standard time in any difficulty display, including the standard egg searching time, the standard egg picking time, and the standard transfer time, compare them with the recorded first duration, second duration, and third duration one by one to determine whether any stage in this egg picking stage is qualified; For any single complete egg picking stage, If the first duration is less than or equal to the standard egg searching time, the central control module determines that this egg searching stage is qualified and controls the counting module to count the number of qualified times in the egg searching stage; If the first duration is greater than the standard egg searching time, the central control module determines that this egg searching stage is unqualified; If the second duration is less than or equal to the standard egg picking time, the central control module determines that this egg picking stage is qualified and controls the counting module to count the number of qualified times in the egg picking stage; If the second duration is greater than the standard egg picking time, the central control module determines that this egg picking stage is unqualified; If the third duration is less than or equal to the standard transfer time, the central control module determines that this transfer stage is qualified and controls the counting module to count the number of qualified times in the transfer stage; If the third duration is greater than the standard transfer time, the central control module determines that this transfer stage is unqualified.

6. The egg retrieval training device based on the cumulus-oocyte complex mimic according to claim 5, characterized in that, For any complete egg picking stage, according to the recorded first duration, second duration, and third duration, finally calculate the total duration of this egg picking operation by summarization. There is a standard total time set according to the standard time of each stage in the egg picking stage in the central control module. Compare the sizes of the two to determine whether this egg picking operation is qualified; If the total duration is less than or equal to the standard total time of the egg picking stage, the central control module determines that the total duration of this egg picking operation is qualified, records the duration of this egg picking operation, and makes a qualified judgment on the total duration of the next egg picking operation; If the total duration is greater than the standard total time of the egg retrieval stage, the central control module determines that the current egg retrieval operation is unqualified, records the duration of the current egg retrieval operation, and makes a qualification judgment on the total duration of the next egg retrieval operation; Stop the qualification operation judgment until the duration of the egg retrieval operation is completely judged.

7. The egg retrieval training device based on the corona radiata cumulus oophorus complex imitation according to claim 6, characterized in that, For the egg retrieval stages with multiple operations by the same trainee, for any complete egg retrieval stage among them, display the status according to the different difficulties it is in, conduct a single scoring one by one, and calculate the overall score based on the average value of the overall egg retrieval duration and the number of unqualified times in the overall egg retrieval stage. In the central control machine module, there are qualified scores and good scores for the overall egg retrieval stage, and the good score is greater than the qualified score; In the central control module, there is a single difficulty coefficient when displaying each difficulty, including an auxiliary difficulty coefficient for the auxiliary operation state, a non-auxiliary difficulty coefficient for the non-auxiliary operation state, and a interference difficulty coefficient for the interference operation state. The auxiliary difficulty coefficient is the smallest, the interference difficulty coefficient is the largest, the non-auxiliary difficulty coefficient is greater than the auxiliary difficulty coefficient and less than the interference difficulty coefficient; If the overall score is less than the qualified score, it is determined that the training result of the trainee is unqualified; If the overall score is equal to the qualified score, it is determined that the training result of the trainee is qualified; If the overall score is greater than the qualified score and less than or equal to the good score, it is determined that the training result of the trainee is good; If the overall score is greater than the good score, it is determined that the training result of the trainee is excellent.

8. The egg retrieval training device based on the oocyte-cumulus complex mimic according to claim 7, characterized in that, For the egg retrieval stages with multiple operations by the same trainee, calculate the actual egg searching qualification ratio, actual egg picking qualification ratio, and actual transfer qualification ratio according to the qualified times in each stage. In the central control module, there is a standard egg retrieval stage qualification ratio, including a standard egg searching stage qualification ratio, a standard egg picking stage qualification ratio, and a standard transfer stage qualification ratio; When the actual egg searching stage qualification ratio, actual egg picking stage qualification ratio, and actual transfer stage qualification ratio are respectively greater than or equal to their corresponding standard egg retrieval stage qualification ratios, start to judge the egg retrieval smoothness rate of the egg retrieval stage; When any one of the actual egg searching stage qualification ratio, actual egg picking stage qualification ratio, and actual transfer stage qualification ratio is less than its corresponding standard egg retrieval stage qualification ratio, the central control module marks the item corresponding to this trainee as a key training item and displays it on the third eyepiece.

9. The egg retrieval training device based on the cumulus-oocyte complex mimic according to claim 8, characterized in that, For the egg retrieval stages with multiple operations by the same trainee, calculate the egg retrieval smoothness rate of the egg retrieval stage according to the different difficulty display states and the recorded first duration, second duration, and third duration, and judge the stability degree of the trainee's egg retrieval stage. In the central control module, there is a first standard egg retrieval smoothness rate during auxiliary operation, a second standard egg retrieval smoothness rate during non-auxiliary operation, and a third standard egg retrieval smoothness rate during interference operation. The first standard egg retrieval smoothness rate is the smallest, the third standard egg retrieval smoothness rate is the largest, the second standard egg retrieval smoothness rate is greater than the first standard egg retrieval smoothness rate and less than the third standard egg retrieval smoothness rate; For the egg retrieval stages with multiple operations by the same trainee, In the assisted operation, if the egg picking smoothness rate is greater than or equal to the first standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is stable; if the egg picking smoothness rate is less than the first standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is unstable; In the non-assisted operation, if the egg picking smoothness rate is greater than or equal to the second standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is stable; if the egg picking smoothness rate is less than the second standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is unstable; In the interference operation, if the egg picking smoothness rate is greater than or equal to the third standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is stable; if the egg picking smoothness rate is less than the third standard egg picking smoothness rate, the central control module determines that the egg picking operation of the training personnel is unstable.

10. The oocyte retrieval training device based on the cumulus-oocyte complex mimic according to claim 9, characterized in that, The central control module can arrange the actual total egg picking time of all training personnel from largest to smallest; when the actual total egg picking time of the training personnel is equal, the central control module arranges the training personnel from largest to smallest according to the egg picking smoothness rate in their picking stages, and displays the final arrangement order on the third eyepiece.

Images