Development method and application of KASP markers for chrysanthemum with osmanthus flower type

The association sites of chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum breeding methods were identified through BSA-seq technology and converted into KASP markers, which solved the problems of low efficiency and poor orientation of traditional chrysanthemum osmanthus-shaped chrysanthemum breeding methods, and achieved early efficient screening and selection of chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum osmanthus-shaped chrysanthemum o

CN117248070BActive Publication Date: 2025-06-03NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202311333774.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-10-16
Publication Date
2025-06-03
Estimated Expiration
2043-10-16

AI Technical Summary

Technical Problem

Traditional chrysanthemum breeding methods have limitations such as long cycle, low efficiency, and undirectionality, making it difficult to achieve efficient early screening and selection of osmanthus-supporting types.

Method used

BSA-seq technology quickly identified the chrysanthemum osmanthus-type association sites, and converted the SNP and InDel association sites into KASP markers to realize the early identification of the chrysanthemum osmanthus-type.

Benefits of technology

This method can complete the selection of large-scale osmanthus-supported osmanthus-type patterns in a short time, avoiding the cumbersomeness of phenotypic observations in the field of flowering, reducing the impact of environmental factors on phenotypic identification, and greatly shortening the breeding cycle.

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Abstract

The present invention discloses a method for developing KASP markers of chrysanthemum receptacle osmanthus type and its application. The present invention rapidly identifies chrysanthemum receptacle osmanthus type associated loci through BSA-seq based on whole-genome resequencing, and converts SNP and InDel associated loci into KASP markers. Compared with traditional PCR- and electrophoresis-based molecular markers, KASP markers have obvious advantages such as high conversion success rate, accurate genotyping, low development cost, strong flexibility, and easy automation. The developed KASP-Chr6__179207697-G / A has an identification accuracy rate of 76-85%, and can complete the screening of a large number of materials for receptacle osmanthus type in a short time, avoiding cumbersome field phenotyping observations during the flowering period and reducing the influence of environmental factors on phenotypic identification, thus greatly shortening the breeding cycle. The present invention lays an important foundation for realizing early large-scale, high-throughput, and automated detection and screening of chrysanthemum receptacle osmanthus type, and has important theoretical and practical significance for MAS breeding of chrysanthemum flower type.
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