Edible mushroom yield increasing agent and application thereof

Abstract

The application discloses an edible mushroom yield increasing agent and application thereof, and raw materials used include soybean meal 20-40 parts, mushroom increasing peptide 20-25 parts, potassium fulvate 15-20 parts and mineral by-product K1 10-15 parts. Each raw material has the effect of promoting mycelium growth, and after mixing, each raw material takes the advantages of others, and not only does not reduce the effect of each raw material, but on the contrary, the prepared edible mushroom yield increasing agent increases the effect of promoting growth, each raw material is reasonably matched, and the effect of coordination and yield increase is achieved. The edible mushroom yield increasing agent has a wide application range, can improve the fungus growth rate and increase the yield of various edible mushrooms such as pleurotus ostreatus, tricholoma giganteum, lentinula edodes, pleurotus eryngii, deer mushroom, hypsizygus marmoreus and flammulina velutipes, can increase the protein content of edible mushroom products by 6.6-17.9%, and can increase the content of part of amino acids, so that the nutritional characteristics of edible mushroom products are improved.

Description

Technical Field

[0001] This invention belongs to the field of edible fungi cultivation technology, specifically relating to an edible fungi yield enhancer and its application. Background Technology

[0002] Edible fungi are rich in nutrients, with thick, fleshy textures and unique flavors, making them a popular food source. They contain protein, carbohydrates, vitamins, trace elements, minerals, and various amino acids essential for the human body. Consuming more fungi can enhance the body's immunity. Common edible fungi include shiitake mushrooms, straw mushrooms, button mushrooms, wood ear mushrooms, silver ear mushrooms, monkey head mushrooms, bamboo fungus, matsutake mushrooms, button mushrooms, red mushrooms, and porcini mushrooms. Currently, with the increasing consumption and expanding production scale of edible fungi, the most significant challenge for producers is intensified competition. To maintain good profits, producers must continuously increase yield and marketability. This necessitates adding nitrogen sources, trace elements, and even compound fertilizers to the cultivation substrate to increase production, thereby improving nutritional value and economic benefits.

[0003] The cultivation substrate is the nutrient source for the growth of edible fungi fruiting bodies, and its formulation is crucial in edible fungi production. Currently, cultivation substrates mainly use sawdust, cottonseed hulls, and corn cobs as raw materials, supplemented with wheat bran or rice bran. However, to promote mycelial growth and increase yield, the cultivation substrate needs to include nitrogen-rich sources such as soybean meal and soybean flour, which increases production costs to some extent. Current fertilizer formulas for supplementing edible fungi cultivation primarily focus on nitrogen, phosphorus, calcium, and sulfur, neglecting the supply of other essential nutrients and micronutrients. Although the cultivation substrate contains macronutrients such as carbon, nitrogen, phosphorus, and potassium, as well as some micronutrients, these require a period of time (5-7 days) after inoculation to be released and utilized by the mycelium. Furthermore, the insufficient content of some nutrients hinders early mycelial growth, leading to slow growth, increased susceptibility to contamination by other microorganisms, cultivation failure, slow mycelial growth, and delayed fruiting. Insufficient nutrient supply in the later stages of growth results in decreased yield. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide an edible fungus yield enhancer and its application, so as to solve the technical problems of slow mycelial growth rate and low yield of edible fungi.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: An edible fungus yield-enhancing agent is provided, comprising the following raw materials by weight: 20-40 parts soybean meal, 20-25 parts mushroom-enhancing peptide, 15-20 parts potassium humate, and 10-15 parts mineral by-product K1, wherein the mineral by-product K1 comprises 3 × 10⁻⁶ total nitrogen. 3 -4×10 3 mg / kg, total carbon 6×104 -7×10 4 mg / kg, humic acid 10 5 -2×10 5 mg / kg, Ca 6×10 3 -8×10 3 mg / kg, K 2×10 4 -3×10 4 mg / kg, P 600-800mg / kg, B 70-90mg / kg, Na 10 4 -2×10 4 mg / kg, Mg 10 3 -2×10 3 mg / kg, Al 5×10 3 -6×10 3 mg / kg, Ti 80-130mg / kg, V6-9mg / kg, Mn 100-160mg / kg, Fe 2×10 4 -3×10 4 mg / kg, Zn 10-20mg / kg, Sr 100-200mg / kg, Co 1-2.1mg / kg, Cu 3-4mg / kg, Mo 3-4mg / kg and Se 1-1.6mg / kg.

[0006] Among the above raw materials, soybean meal serves as a nitrogen source, primarily a slow-release nitrogen source, which can meet the nitrogen requirements of mycelia. Pichia pastoris, a fermentation extract, contains abundant yeast metabolites and primarily acts as a fast-release nitrogen source. It also meets the growth factor requirements of edible fungi mycelia, significantly accelerating mycelial growth and promoting thicker, whiter, and more robust mycelia. Potassium humate is a highly efficient fertilizer, commonly used as a plant growth regulator and fertilizer to improve plant resistance, promote root growth, increase nutrient absorption, and improve soil quality; it also promotes the growth of edible fungi mycelia. K1, a mineral byproduct, contains many trace elements and can serve as an essential component of coenzymes, meeting the trace element requirements of mycelia and promoting mycelial growth.

[0007] Based on the above technical solution, the present invention can be further improved as follows:

[0008] Furthermore, the product comprises the following raw materials by weight: 30 parts soybean meal, 23 parts mushroom-enhancing peptides, 17 parts potassium humate, and 12 parts mineral by-product K1, wherein mineral by-product K1 includes 3.12 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6.88×10 4 mg / kg, humic acid 1.67×10 5 mg / kg, Ca 7.13×10 3mg / kg, K 2.83×10 4 mg / kg, P 638mg / kg, B 80.8mg / kg, Na 1.54×10 4 mg / kg, Mg 1.43×10 3 mg / kg, Al 5.39×10 3 mg / kg, Ti 108mg / kg, V 7.79mg / kg, Mn 146mg / kg, Fe 2.8×10 4 mg / kg, Zn 15.8mg / kg, Sr 141mg / kg, Co 1.85mg / kg, Cu 3.72mg / kg, Mo3.29mg / kg and Se 1.3mg / kg.

[0009] Furthermore, the product comprises the following raw materials by weight: 20 parts soybean meal, 25 parts mushroom-enhancing peptides, 15 parts potassium humate, and 15 parts mineral by-product K1, wherein mineral by-product K1 includes 4 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6×10 4 mg / kg, humic acid 2×10 5 mg / kg, Ca 6×10 3 mg / kg, K 2×10 4 mg / kg, P 800mg / kg, B 70mg / kg, Na 10 4 mg / kg, Mg 2×10 3 mg / kg, Al 5×10 3 mg / kg, Ti 130mg / kg, V 6mg / kg, Mn 160mg / kg, Fe 2×10 4 mg / kg, Zn 20mg / kg, Sr 200mg / kg, Co 1mg / kg, Cu 4mg / kg, Mo 3mg / kg and Se 1mg / kg.

[0010] Furthermore, the product comprises the following raw materials by weight: 40 parts soybean meal, 20 parts mushroom-enhancing peptide, 20 parts potassium humate, and 10 parts mineral by-product K1, wherein mineral by-product K1 includes 3 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 7×10 4 mg / kg, humic acid 10 5 mg / kg, Ca 8×10 3 mg / kg, K 3×10 4 mg / kg, P 600mg / kg, B 90mg / kg, Na 2×10 4 mg / kg, Mg10 3 mg / kg, Al 6×103 mg / kg, Ti 80mg / kg, V 9mg / kg, Mn 160mg / kg, Fe 3×10 4 mg / kg, Zn 10mg / kg, Sr 100mg / kg, Co 2.1mg / kg, Cu 4mg / kg, Mo 3mg / kg and Se 1.6mg / kg.

[0011] Furthermore, soybean meal has a moisture content of 8-13% and a crude protein content of 42-60%.

[0012] Furthermore, the water solubility of the mushroom-enhancing peptide is ≥99.9%, and the content of acid-soluble protein is 20-35%.

[0013] Furthermore, potassium fulvate contains 50-65% humic acid, 12-20% potassium oxide, and has a pH of 8-10.

[0014] Furthermore, potassium fulvate contains 60% humic acid, 16% potassium oxide, and has a pH of 9.

[0015] This invention also discloses the application of a mushroom yield enhancer in mushroom cultivation, comprising the following steps: weighing the raw materials of the mushroom yield enhancer according to the proportion, and then mixing them evenly; mixing the prepared mushroom yield enhancer with the cultivation raw materials for 5-10 minutes, and then adding water and stirring for 10-30 minutes to obtain the cultivation substrate; after sterilizing, cooling, inoculating, mycelial cultivating and managing the fruiting of the cultivation substrate, the mushrooms can be harvested; the mass ratio of mushroom yield enhancer, cultivation raw materials and water is 1.5-2.5:100:110-120.

[0016] Based on the above technical solution, the present invention can be further improved as follows:

[0017] Furthermore, the mass ratio of edible fungus yield enhancer, cultivation raw materials, and water is 2:100:120.

[0018] The present invention has the following beneficial effects:

[0019] 1. Excellent mycelial growth effect: After using the edible fungi yield enhancer formulated in this invention, the mycelial growth rate of king oyster mushrooms, oyster mushrooms, and large-cap mushrooms can be increased by 7.5-15.3%, and the mycelium becomes significantly denser, thicker, and whiter in color.

[0020] 2. Good yield-increasing effect: After using the edible fungus yield-increasing agent formulated in this invention, the yield of edible fungi products can be increased by 6-8%, and the commercial mushroom yield can be increased by 2-5%.

[0021] 3. Using this edible fungus yield enhancer can increase the protein content of edible fungus products by 6.6-17.9% and increase the content of some amino acids, thereby improving the nutritional characteristics of edible fungus products.

[0022] 4. It has a wide range of applications in edible fungi production, and can improve the mycelium growth rate and increase the yield of various edible fungi such as oyster mushrooms, king oyster mushrooms, shiitake mushrooms, king oyster mushrooms, velvet mushrooms, enoki mushrooms, and golden needle mushrooms.

[0023] 5. All the yield enhancers are made from natural raw materials and contain no chemically synthesized substances. Edible fungi products using this yield enhancer can meet green standards and can be applied in organic production. Detailed Implementation

[0024] The principles and features of this invention are described below. The examples given are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer should be followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0025] Of the following raw materials, soybean meal is commercial soybean meal for production, purchased from Beijing Guchuan Flour Group; mushroom-enhancing peptides were purchased from Hebei Guyou Agricultural Technology Service Co., Ltd.; potassium humate was purchased from Shandong Shizhaofeng Agricultural Science and Technology Co., Ltd.; and mineral by-product K1 was purchased from Innovation Trading Co., Ltd.

[0026] Example 1:

[0027] An edible fungus yield enhancer comprises, by weight, the following raw materials: 30 parts soybean meal, 23 parts mushroom-enhancing peptide, 17 parts potassium humate, and 12 parts mineral by-product K1, wherein mineral by-product K1 comprises 3.12 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6.88×10 4 mg / kg, humic acid 1.67×10 5 mg / kg, Ca 7.13×10 3 mg / kg, K 2.83×10 4 mg / kg, P 638mg / kg, B80.8mg / kg, Na 1.54×10 4 mg / kg, Mg 1.43×10 3 mg / kg, Al 5.39×10 3 mg / kg, Ti 108mg / kg, V 7.79mg / kg, Mn 146mg / kg, Fe 2.8×10 4The concentrations of zinc, ...

[0028] This embodiment also discloses the application of a mushroom yield enhancer in the cultivation of king oyster mushrooms, including the following steps: weighing the raw materials of the mushroom yield enhancer according to the proportion, and then mixing them evenly; mixing the prepared mushroom yield enhancer with the cultivation raw materials for 8 minutes, and then adding water and stirring for 20 minutes to obtain the cultivation substrate; sterilizing and cooling the cultivation substrate, and then inoculating it with king oyster mushroom mycelium for 65 days, and harvesting king oyster mushrooms after fruiting management; the mass ratio of mushroom yield enhancer, cultivation raw materials and water is 2:100:120.

[0029] Example 2:

[0030] A yield-enhancing agent for edible fungi comprises, by weight, the following raw materials: 20 parts soybean meal, 25 parts mushroom-enhancing peptide, 15 parts potassium humate, and 15 parts mineral by-product K1, wherein mineral by-product K1 comprises 4 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6×10 4 mg / kg, humic acid 2×10 5 mg / kg, Ca 6×10 3 mg / kg, K 2×10 4 mg / kg, P 800mg / kg, B 70mg / kg, Na 10 4 mg / kg, Mg 2×10 3 mg / kg, Al 5×10 3 mg / kg, Ti 130mg / kg, V 6mg / kg, Mn 160mg / kg, Fe 2×10 4 The components are: mg / kg Zn 20 mg / kg, Sr 200 mg / kg, Co 1 mg / kg, Cu 4 mg / kg, Mo 3 mg / kg, and Se 1 mg / kg. The soybean meal has a moisture content of 8% and a crude protein content of 60%. The water solubility of the mushroom-enhancing peptide is 99.95%, and the acid-soluble protein content is 20%. The potassium humate contains 65% humic acid, 12% potassium oxide, and has a pH of 10.

[0031] This embodiment also discloses the application of a mushroom yield enhancer in oyster mushroom cultivation, including the following steps: weighing the raw materials of the mushroom yield enhancer according to the proportion, and then mixing them evenly; mixing the prepared mushroom yield enhancer with the cultivation raw materials for 10 minutes, and then adding water and stirring for 10 minutes to obtain the cultivation substrate; sterilizing and cooling the cultivation substrate, and then inoculating it with oyster mushroom mycelium for 130 days, after which the oyster mushrooms can be harvested; the mass ratio of mushroom yield enhancer, cultivation raw materials and water is 1.5:100:115.

[0032] Example 3:

[0033] An edible fungus yield enhancer comprises, by weight, the following raw materials: 40 parts soybean meal, 20 parts mushroom-enhancing peptide, 20 parts potassium humate, and 10 parts mineral by-product K1, wherein mineral by-product K1 comprises 3 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 7×10 4 mg / kg, humic acid 10 5 mg / kg, Ca 8×10 3 mg / kg, K 3×10 4 mg / kg, P 600mg / kg, B 90mg / kg, Na 2×10 4 mg / kg, Mg10 3 mg / kg, Al 6×10 3 mg / kg, Ti 80mg / kg, V 9mg / kg, Mn 160mg / kg, Fe 3×10 4 The concentrations of zinc, ...

[0034] This embodiment also discloses the application of an edible fungus yield enhancer in the cultivation of *Stropharia masticata*, including the following steps: weighing the raw materials of the edible fungus yield enhancer according to the proportion, and then mixing them evenly; mixing the prepared edible fungus yield enhancer with the cultivation raw materials for 5 minutes, and then adding water and stirring for 30 minutes to obtain the cultivation substrate; sterilizing and cooling the cultivation substrate, and then inoculating it with *Stropharia masticata* mycelium for 45 days, and harvesting *Stropharia masticata* after fruiting management; the mass ratio of edible fungus yield enhancer, cultivation raw materials and water is 2.5:100:110.

[0035] Comparative Example 1:

[0036] The cultivation of king oyster mushrooms includes the following steps: add water to the cultivation material and stir for 20 minutes to obtain the cultivation substrate. The mass ratio of cultivation material to water is 100:120. Sterilize and cool the cultivation substrate, then inoculate it with king oyster mushroom mycelium and cultivate it for 65 days. After fruiting management, the king oyster mushrooms can be harvested.

[0037] Comparative Example 2:

[0038] An edible fungus yield enhancer comprises, by weight, the following raw materials: 30 parts soybean meal, 17 parts potassium humate, and 12 parts mineral by-product K1, wherein mineral by-product K1 comprises 3.12 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6.88×10 4 mg / kg, humic acid 1.67×10 5 mg / kg, Ca 7.13×10 3 mg / kg, K 2.83×10 4 mg / kg, P 638mg / kg, B 80.8mg / kg, Na1.54×10 4 mg / kg, Mg 1.43×10 3 mg / kg, Al 5.39×10 3 mg / kg, Ti 108mg / kg, V 7.79mg / kg, Mn 146mg / kg, Fe 2.8×10 4 The concentrations of zinc, ...

[0039] This comparative example also includes the application of edible fungi yield enhancer in king oyster mushroom cultivation, including the following steps: weigh the raw materials of the edible fungi yield enhancer according to the proportion, and then mix them evenly to obtain the product; mix the prepared edible fungi yield enhancer with the cultivation raw materials for 8 minutes, and then add water and stir for 20 minutes to obtain the cultivation substrate. The mass ratio of edible fungi yield enhancer, cultivation raw materials and water is 2:100:120; sterilize and cool the cultivation substrate, and then inoculate it with king oyster mushroom mycelium for 65 days. After fruiting management, the king oyster mushrooms can be harvested.

[0040] Comparative Example 3:

[0041] The cultivation of oyster mushrooms includes the following steps: add water to the cultivation material and stir for 10 minutes to obtain the cultivation substrate. The mass ratio of cultivation material to water is 100:115. Sterilize and cool the cultivation substrate, then inoculate it with oyster mushroom mycelium and cultivate it for 130 days. After fruiting management, the oyster mushrooms can be harvested.

[0042] Comparative Example 4:

[0043] An edible fungus yield enhancer comprises, by weight, the following raw materials: 20 parts soybean meal, 25 parts mushroom-enhancing peptide, and 15 parts mineral by-product K1, wherein mineral by-product K1 comprises 4 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6×10 4 mg / kg, humic acid 2×10 5 mg / kg, Ca6×10 3 mg / kg, K 2×10 4 mg / kg, P 800mg / kg, B 70mg / kg, Na 10 4 mg / kg, Mg 2×10 3 mg / kg, Al 5×10 3 mg / kg, Ti 130mg / kg, V 6mg / kg, Mn 160mg / kg, Fe 2×10 4 The components are: mg / kg, Zn 20mg / kg, Sr 200mg / kg, Co 1mg / kg, Cu 4mg / kg, Mo 3mg / kg, and Se 1mg / kg. The soybean meal has a moisture content of 8% and a crude protein content of 60%. The water solubility of the mushroom-enhancing peptide is 99.95%, and the acid-soluble protein content is 20%.

[0044] This comparative example also includes the application of edible fungi yield enhancer in oyster mushroom cultivation, including the following steps: weigh the raw materials of the edible fungi yield enhancer according to the proportion, and then mix them evenly to obtain the product; mix the prepared edible fungi yield enhancer with the cultivation raw materials for 10 minutes, and then add water and stir for 10 minutes to obtain the cultivation substrate. The mass ratio of edible fungi yield enhancer, cultivation raw materials and water is 1.5:100:115; sterilize and cool the cultivation substrate, and then inoculate it with oyster mushroom mycelium for 130 days. After fruiting management, the oyster mushrooms can be harvested.

[0045] Comparative Example 5:

[0046] The cultivation of giant king oyster mushrooms includes the following steps: add water to the cultivation material and stir for 30 minutes to obtain the cultivation substrate. The mass ratio of cultivation material to water is 100:110. Sterilize and cool the cultivation substrate, then inoculate it with giant king oyster mushroom mycelium and cultivate for 45 days. After fruiting management, the giant king oyster mushrooms can be harvested.

[0047] Comparative Example 6:

[0048] An edible fungus yield enhancer comprises the following raw materials by weight: 40 parts soybean meal, 20 parts mushroom-enhancing peptide, and 20 parts potassium humate. The soybean meal has a moisture content of 13% and a crude protein content of 42%; the mushroom-enhancing peptide has a water solubility of 99.99% and an acid-soluble protein content of 35%; the potassium humate contains 50% humic acid, 20% potassium oxide, and has a pH of 8.

[0049] This comparative example also includes the application of edible fungi yield enhancer in the cultivation of *Stropharia masticata*, including the following steps: weigh the raw materials of the edible fungi yield enhancer according to the proportion, and then mix them evenly to obtain the product; mix the prepared edible fungi yield enhancer with the cultivation raw materials for 5 minutes, then add water and stir for 30 minutes to obtain the cultivation substrate, with the mass ratio of edible fungi yield enhancer, cultivation raw materials and water being 2.5:100:110; sterilize and cool the cultivation substrate, then inoculate it with *Stropharia masticata* mycelium and cultivate it for 45 days. After fruiting management, *Stropharia masticata* can be harvested.

[0050] Results analysis:

[0051] 1. The results of the comparison of mycelial growth between Examples 1-3 and Comparative Examples 1-6 are shown in Table 1.

[0052] Table 1. Effects of adding edible fungi yield enhancers on the mycelial growth of edible fungi.

[0053]

[0054]

[0055] In Example 1, after using this edible fungus yield enhancer, the mycelial growth rate of King Oyster Mushroom reached 0.32 cm / d, which was 14.3% higher than that of Comparative Example 1 and 6.67% higher than that of Comparative Example 2; in Example 2, the mycelial growth rate of Oyster Mushroom reached 0.38 cm / d, which was 7.5% higher than that of Comparative Example 3 and 5.56% higher than that of Comparative Example 4; in Example 3, the mycelial growth rate of Giant King Mushroom reached 0.27 cm / d, which was 15.3% higher than that of Comparative Example 5 and 8% higher than that of Comparative Example 6; and the mycelia of the three edible fungi became significantly denser, thicker, and whiter in color.

[0056] 2. The effects of Examples 1-3 and Comparative Examples 1-6 on edible fungi yield are shown in Tables 2 and 3.

[0057] Table 2. Effects of adding edible fungi yield enhancers on the yield of king oyster mushrooms and oyster mushrooms.

[0058]

[0059] In Example 1, after using this edible fungus yield enhancer, the average yield of king oyster mushrooms per bag (bag fold width * length 18cm * 35cm) reached 527g, which is an increase of 6.5% compared with Comparative Example 1 and an increase of 4.1% compared with Comparative Example 2. The average yield of marketable mushrooms per bag reached 475g, and the marketable mushroom rate reached 90.1%, which is an increase of 3.3% compared with Comparative Example 1 and an increase of 1% compared with Comparative Example 2.

[0060] In Example 2, the average yield per bag of oyster mushrooms (bag fold width * length 22cm * 35cm) reached 875g, an increase of 6.3% compared to Comparative Example 3 and 2.7% compared to Comparative Example 4. The average yield per bag of marketable mushrooms reached 847g, with a marketable mushroom rate of 96.8%, an increase of 2% compared to Comparative Example 3 and 0.6% compared to Comparative Example 4. This indicates that the ingredients in the edible fungi yield enhancer are rationally formulated and can achieve a synergistic effect.

[0061] Table 3. Effects of adding edible fungi yield enhancers on the yield of *Stropharia macrocarpa*.

[0062]

[0063] In Example 3, after using this edible fungus yield enhancer, the average yield of *Stropharia carota* per square meter (feeding amount 25kg / ㎡) reached 10250g, which is an 8% increase compared to Comparative Example 5 and a 2.9% increase compared to Comparative Example 6. The average yield of marketable mushrooms per square meter reached 7636g, and the marketable mushroom rate reached 74.5%, which is an increase of 5.3% compared to Comparative Example 5 and an increase of 2.3% compared to Comparative Example 6.

[0064] 3. The results of the influence of Examples 1-3 and Comparative Examples 1-6 on the protein content of edible fungi are shown in Table 4.

[0065] Table 4. Effects of adding edible fungus yield enhancers on protein content in edible fungi.

[0066]

[0067] In Example 1, after using this edible fungus yield enhancer, the protein content of the fruiting body of King Oyster Mushroom reached 1.82g / 100g, which was 6.6% higher than that of Comparative Example 1; in Example 2, the protein content of the fruiting body of Oyster Mushroom reached 1.79g / 100g, which was 8.2% higher than that of Comparative Example 3; in Example 3, the protein content of the fruiting body of Giant King Mushroom reached 2.30g / 100g, which was 17.9% higher than that of Comparative Example 5.

[0068] 4. Taking *Agaricus bisporus* as an example, the effect of this edible fungus additive on the free amino acid content in edible fungi was investigated. The results of the effects on the protein content in edible fungi of Examples 5, 6 and Example 3 are shown in Table 5.

[0069] Table 5. Effects of adding edible fungi yield-enhancing agents on 14 free amino acids in the fruiting bodies of *Pleurotus ostreatus*.

[0070]

[0071] As can be seen from Table 5, after using this edible fungus yield enhancer in Example 3, the content of some amino acids can be increased, and the content of 14 kinds of total free amino acids can be increased, which is 16.15% higher than that of Comparative Example 5.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A yield-enhancing agent for edible fungi, characterized in that, The ingredients, by weight, include: 20-40 parts soybean meal, 20-25 parts mushroom-enhancing peptides, 15-20 parts potassium humate, and 10-15 parts mineral by-product K1, wherein the mineral by-product K1 comprises 3 × 10⁻⁶ total nitrogen. 3 -4×10 3 mg / kg, total carbon 6×10 4 -7×10 4 mg / kg, humic acid 10 5 -2×10 5 mg / kg, Ca 6×10 3 -8×10 3 mg / kg, K 2×10 4 -3×10 4 mg / kg, P 600-800 mg / kg, B 70-90mg / kg, Na 10 4 -2×10 4 mg / kg, Mg 10 3 -2×10 3 mg / kg, Al 5×10 3 -6×10 3 mg / kg, Ti 80-130 mg / kg, V 6-9 mg / kg, Mn 100-160 mg / kg, Fe 2×10 4 -3×10 4 The potassium fulvate contains 10-20 mg / kg of Zn, 100-200 mg / kg of Sr, 1-2.1 mg / kg of Co, 3-4 mg / kg of Cu, 3-4 mg / kg of Mo, and 1-1.6 mg / kg of Se; the potassium fulvate contains 50-65% humic acid, 12-20% potassium oxide, and has a pH of 8-10. The application of the edible fungus yield enhancer in edible fungus cultivation includes the following steps: weighing each raw material of the edible fungus yield enhancer according to the proportion, and then mixing them evenly; mixing the prepared edible fungus yield enhancer with the cultivation raw material for 5-10 minutes, and then adding water and stirring for 10-30 minutes to obtain the cultivation substrate; after sterilizing, cooling, inoculating, mycelial cultivating and managing the fruiting of the cultivation substrate, the edible fungi can be harvested; the mass ratio of the edible fungus yield enhancer, cultivation raw material and water is 1.5-2.5:100:110-120.

2. The edible fungus yield enhancer according to claim 1, characterized in that, The product comprises the following raw materials by weight: 30 parts soybean meal, 23 parts mushroom-enhancing peptides, 17 parts potassium humate, and 12 parts mineral by-product K1, wherein the mineral by-product K1 comprises 3.12 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6.88×10 4 mg / kg, humic acid 1.67×10 5 mg / kg, Ca 7.13×10 3 mg / kg, K 2.83×10 4 mg / kg, P 638 mg / kg, B 80.8mg / kg, Na 1.54×10 4 mg / kg, Mg 1.43×10 3 mg / kg, Al 5.39×10 3 mg / kg, Ti 108 mg / kg, V 7.79 mg / kg, Mn 146 mg / kg, Fe 2.8×10 4 mg / kg, Zn15.8 mg / kg, Sr 141 mg / kg, Co 1.85 mg / kg, Cu 3.72 mg / kg, Mo 3.29 mg / kg and Se 1.3 mg / kg.

3. The edible fungus yield enhancer according to claim 1, characterized in that, The ingredients, by weight, include: 20 parts soybean meal, 25 parts mushroom-enhancing peptides, 15 parts potassium humate, and 15 parts mineral by-product K1, wherein the mineral by-product K1 comprises 4 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 6×10 4 mg / kg, humic acid 2×10 5 mg / kg, Ca 6×10 3 mg / kg, K 2×10 4 mg / kg, P 800mg / kg, B 70mg / kg, Na 10 4 mg / kg, Mg 2×10 3 mg / kg, Al 5×10 3 mg / kg, Ti 130 mg / kg, V 6mg / kg, Mn 160 mg / kg, Fe 2×10 4 mg / kg, Zn 20 mg / kg, Sr 200 mg / kg, Co 1 mg / kg, Cu 4mg / kg, Mo 3 mg / kg and Se 1 mg / kg.

4. The edible fungus yield enhancer according to claim 1, characterized in that, The ingredients, by weight, include: 40 parts soybean meal, 20 parts mushroom-enhancing peptides, 20 parts potassium humate, and 10 parts mineral by-product K1, wherein the mineral by-product K1 comprises 3 × 10⁻⁶ total nitrogen. 3 mg / kg, total carbon 7×10 4 mg / kg, humic acid 10 5 mg / kg, Ca 8×10 3 mg / kg, K 3×10 4 mg / kg, P 600 mg / kg, B 90mg / kg, Na 2×10 4 mg / kg, Mg 10 3 mg / kg, Al 6×10 3 mg / kg, Ti 80 mg / kg, V 9 mg / kg, Mn 160 mg / kg, Fe 3×10 4 mg / kg, Zn 10 mg / kg, Sr 100 mg / kg, Co 2.1 mg / kg, Cu 4mg / kg, Mo 3 mg / kg and Se 1.6 mg / kg.

5. The edible fungus yield enhancer according to any one of claims 1-4, characterized in that: The soybean meal has a moisture content of 8-13% and a crude protein content of 42-60%.

6. The edible fungus yield enhancer according to any one of claims 1-4, characterized in that: The water solubility of the mushroom-enhancing peptide is ≥99.9%, and the content of acid-soluble protein is 20-35%.

7. The edible fungus yield enhancer according to claim 1, characterized in that: The potassium fulvic acid contains 60% humic acid, 16% potassium oxide, and has a pH of 9.

8. The edible fungus yield enhancer according to claim 1, characterized in that: The mass ratio of the edible fungus yield enhancer, cultivation raw materials, and water is 2:100:120.

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