An anti-glycation and antioxidant composition, anti-aging cream and its preparation method

Abstract

The present invention belongs to the technical field of daily-use cosmetics, and discloses an anti-glycation and antioxidant composition, an anti-aging cream and a preparation method thereof. The anti-glycation and antioxidant composition of the present invention comprises the following components in parts by weight: 0.1-1.5 parts of carnosine hydrochloride, 0.2-10.4 parts of algal extract, and 0.1-12.5 parts of yeast / rice fermentation product filtrate. Compared with the prior art, the anti-aging cream containing the anti-glycation and antioxidant composition of the present invention has excellent anti-glycation and antioxidant effects and good absorbability at the same time, can reduce oxidative and glycation damage, inhibit the degradation of matrix metalloproteinase, thereby improving wrinkles, brightening the skin and promoting the generation of collagen. At the same time, the present invention also has the functions of moisturizing, supplementing filaggrin or underlying fibers of the skin, and exhibits excellent skin conditioning effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily-use cosmetics, and in particular relates to an anti-glycation and antioxidant composition, an anti-aging cream and a preparation method thereof. Background Art

[0002] Skin aging is a complex physiological process, and its clinical manifestations include skin wrinkles, roughness, dryness, skin relaxation and sagging, as well as pigmentation, etc. Human skin aging is divided into endogenous aging and exogenous aging. Endogenous aging is an aging process that occurs naturally with age and is affected by each person's genetic factors, with relatively large individual differences. For example, the human body's metabolism gradually slows down with age, resulting in an enhanced non-enzymatic glycosylation reaction, and the continuous accumulation of advanced glycation end products (AGEs) in the body, leading to the cross-linking of collagen and elastin in the dermis layer participating in the glycation reaction, causing the skin elasticity to decline, affecting its normal function, and thus appearing wrinkles and various aging phenomena. In addition, AGEs can form pigment adducts with proteins, resulting in skin pigmentation and dull yellow skin color.

[0003] Exogenous aging refers to the aging changes caused by the influence of environmental factors (mainly ultraviolet radiation) on the skin. When the skin is exposed to ultraviolet and blue light for a long time, it will produce a large amount of reactive oxygen species in the skin, causing a series of oxidative damage reactions, such as mitochondrial damage and apoptosis, and DNA strand breakage in the cell nucleus. At the same time, reactive oxygen species will also stimulate cells to secrete a large number of cytokines, such as matrix metalloproteinases, thereby destroying collagen synthesis and skin barrier damage, etc., further accelerating skin aging. More and more studies have shown that antioxidant is an important means to prevent aging. Therefore, if excessive oxidative free radicals can be eliminated, the skin aging caused by free radicals can be effectively prevented. In addition, a large number of free radicals generated by oxidation continuously accumulate in the body and will also participate in the glycation reaction, thereby further accelerating the formation of AGEs. Therefore, adding antioxidants can not only effectively block the oxidative chain reaction, slow down the generation of free radicals, but also effectively inhibit the generation of AGEs during the glycation process, further improving the anti-glycation effect.

[0004] However, the existing anti-aging products on the market at present cannot simultaneously take into account the anti-glycation and antioxidant effects, have a low absorption rate, insignificant curative effects, and complex components. Therefore, there is still a need for a cream product with excellent anti-glycation and antioxidant effects and capable of inhibiting dull yellow skin color. Summary of the Invention

[0005] The purpose of the present invention is to provide an anti-glycation and antioxidant composition, an anti-aging cream and a preparation method thereof in order to overcome the defects existing in the above-mentioned prior art.

[0006] The object of the present invention can be achieved by the following technical solutions: An anti-glycation and antioxidant composition, comprising the following components in parts by weight: 0.1 to 1.5 parts of carnosine hydrochloride, 0.2 to 10.4 parts of algal extract, and 0.1 to 12.5 parts of yeast / rice fermentation product filtrate.

[0007] As a further preference of the present invention, the anti-glycation and antioxidant composition consists of the following components in parts by weight: 0.2 to 1.2 parts of carnosine hydrochloride, 0.4 to 8.2 parts of algal extract, and 1.0 to 10.4 parts of yeast / rice fermentation product filtrate.

[0008] As a further preference of the present invention, the anti-glycation and antioxidant composition consists of the following components in parts by weight: 0.4 to 1.0 parts of carnosine hydrochloride, 1.0 to 6.0 parts of algal extract, and 2.0 to 9.0 parts of yeast / rice fermentation product filtrate.

[0009] As a further preference of the present invention, the anti-glycation and antioxidant composition comprises the following components in parts by weight: 0.5 to 1.0 parts of carnosine hydrochloride, 2.1 to 4.5 parts of algal extract, and 5.0 to 7.0 parts of yeast / rice fermentation product filtrate.

[0010] Carnosine hydrochloride is the product of the decarboxylation reaction of carnosine. It not only has the effects of carnosine such as scavenging free radicals, antioxidant, and anti-glycation, but also has more stable properties than carnosine in a specific physiological environment. Carnosine hydrochloride can not only be used for skin yellowing removal and skin tone brightening, but also prevent premature skin aging and maintain a youthful skin state. In addition, carnosine hydrochloride can also be used to counteract the invasion of the skin by adverse environments such as external ultraviolet rays and pollutants, protect DNA, increase cell metabolism, restore cell vitality, and promote the production of collagen.

[0011] The algal extract contains sulfur-rich polysaccharides and can be used as a sulfur donor to promote the production of glutathione. Therefore, it has excellent antioxidant performance, can strengthen the skin's own antioxidant system, reduce oxidative damage, and inhibit the degradation of matrix metalloproteinases, thereby improving wrinkles and brightening the skin. The algal extract is preferably a dinoflagellate extract, which contains rich sulfur polysaccharide substances, can provide a barrier to protect cells from pollution damage, and can also stimulate the production of the scavenger in cells - glutathione, purify cells and delay cell aging at the same time.

[0012] The yeast / rice fermentation product filtrate has a film-forming and moisturizing effect. It can not only help rebuild the barrier function of the skin dermal matrix, but also supplement the skin's own filaggrin or underlying fibers, having a skin conditioning effect. In addition, the yeast / rice fermentation product filtrate is rich in a variety of natural skin-nourishing ingredients and has excellent skin-friendly properties (the contact is smaller than that of water), has good permeability, and can promote the absorption of subsequent active ingredients.

[0013] In one embodiment of the present invention, a method for preparing a filtrate of yeast / rice fermentation product is specifically provided, including the following steps:

[0014] (1) Add water to rice powder, heat and stir to form rice slurry. Add β-glucanase to the rice slurry, with the addition amount of the enzyme being 0.001% - 0.5% of the mass of the rice slurry and the enzyme activity being 150 - 200 U / g, and carry out enzymatic hydrolysis to obtain a rice slurry enzymatic hydrolysate;

[0015] (2) Inoculate the yeast strain Saccharomyces veronae into the rice slurry enzymatic hydrolysate, and the fermentation conditions are: fermentation temperature 20 - 40 °C, fermentation time 24 - 48 hours, pH during fermentation is controlled at 6 - 6.5, and a fermentation broth is obtained after fermentation;

[0016] (3) Concentrate the fermentation broth by reverse osmosis technology to efficiently enrich active substances, and obtain a filtrate of yeast / rice fermentation product after heating and sterilization and cooling and filtration.

[0017] The filtrate of yeast / rice fermentation product contains more than 90 kinds of nutrients including amino acids, polysaccharides, polyamines, nucleic acids, and minerals, can promote the expression of filaggrin, involucrin, and serine palmitoyltransferase, and can also promote the construction of epidermal cells and the increase of natural moisturizing factors, thereby accelerating cell metabolism and delaying endogenous aging.

[0018] As a further preference of the present invention, the present invention also provides an anti-aging cream, and the anti-aging cream contains an anti-glycation and antioxidant composition.

[0019] As a further preference of the present invention, the content of the anti-glycation and antioxidant composition in the anti-aging cream is 0.4 - 24.4 wt%.

[0020] As a further preference of the present invention, the content of the anti-glycation and antioxidant composition in the anti-aging cream is 5 - 15 wt%.

[0021] As a further preference of the present invention, the anti-aging cream further contains at least one of a chelating agent, a humectant, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative.

[0022] As a further preference of the present invention, the chelating agent is disodium EDTA; the humectant comprises at least one of glycerol, sodium hyaluronate, 1,3 - butanediol, trehalose, honey, inositol, urea, panthenol and betaine; the skin conditioner comprises at least one of ceramide, pantothenic acid and p - hydroxyacetophenone; the emollient comprises at least one of squalene, jojoba seed oil, castor oil, shea butter, caprylic / capric triglyceride, cetearyl alcohol, beeswax and shea fruit butter; the emulsifier comprises at least one of lecithin, lanolin, lanolin alcohol, cetearyl alcohol / cetearyl glucoside and glyceryl stearate; the thickener comprises at least one of xanthan gum, carbomer, modified starch and high - molecular polymer; the preservative comprises at least one of phenoxyethanol, ethylhexylglycerin and pentylene glycol.

[0023] As a further preference of the present invention, the cream further comprises the following components in parts by weight: 0.4 - 24.4 parts of anti - glycation and antioxidant composition, 0.01 - 0.04 parts of chelating agent, 7.5 - 17 parts of humectant, 0.5 - 3 parts of skin conditioner, 2 - 7 parts of emollient, 1.2 - 10 parts of emulsifier, 0.1 - 2.0 parts of thickener, and 0.1 - 0.5 parts of preservative.

[0024] As a further preference of the present invention, the preparation method of the anti - aging cream comprises the following steps:

[0025] (1) Add the chelating agent, humectant, thickener and skin conditioner to deionized water, heat to 80 - 90 °C, homogenize and stir for 3 - 5 min to obtain a mixed solution A;

[0026] (2) Heat the emulsifier and emollient to 80 - 90 °C, and after they are completely dissolved, add them to the mixed solution A, homogenize and stir for 3 - 5 min to obtain a mixed solution B;

[0027] (3) Cool the mixed solution B to 40 - 50 °C, add the anti - aging composition of the anti - glycation and antioxidant composition and the preservative, stir for 5 - 15 min, and filter to obtain the anti - aging cream.

[0028] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0029] (1) The present invention contains various active ingredients with antioxidant, anti - glycation and moisturizing effects, such as decarboxylated carnosine hydrochloride, algal extract and yeast / rice fermentation filtrate. These active ingredients cooperate with each other to achieve excellent anti - glycation and antioxidant effects.

[0030] (2) The filtrate of yeast / rice fermentation product is rich in a variety of natural skin-nourishing ingredients and has excellent skin-friendly properties (less contact than water), good permeability, can promote the absorption of active ingredients such as decarboxylated carnosine hydrochloride and algal extract, and better play the role of anti-glycation and antioxidant.

[0031] (3) For the anti-glycation and antioxidant efficacy ingredient composition of the present invention, preferably decarboxylated carnosine hydrochloride and algal extract, through the preferred combination and efficacy test of the cosmetics, the most preferred composition ratio is selected to maximize the anti-glycation and antioxidant effect; at the same time, it is paired with the filtrate of yeast / rice fermentation product to help rebuild the barrier function of the skin dermal matrix and supplement the skin's own filaggrin or underlying fibers. Detailed implementation mode

[0032] The following examples further illustrate various embodiments within the scope of the present invention. These examples are given for illustrative purposes only and are not intended to limit the present invention, as many variations of the present invention can be made without departing from the spirit and scope of the present invention.

[0033] All raw materials used in the present invention are commercially available products unless otherwise specified. The filtrate of yeast / rice fermentation product can be a commercially available product or obtained according to the method disclosed in the present invention. In the following specific examples:

[0034] The decarboxylated carnosine hydrochloride is a product of Sichuan Jisheng Biopharmaceutical Co., Ltd.;

[0035] The algal extract is a product of Gonyaulax microalgae extract of Guangzhou Nuoran Biotechnology Co., Ltd.;

[0036] The filtrate of yeast / rice fermentation product is prepared according to the following method:

[0037] (1) Rice powder is added with water and heated, stirred to form a 30 wt% rice slurry. β-glucanase is added to the rice slurry, and the addition amount of the enzyme is 0.15% of the mass of the rice slurry, and the enzyme activity is 200 U / g. Enzymolysis is carried out to obtain a rice slurry enzymolysis solution;

[0038] (2) The yeast strain saccharomyces veronae is inoculated into the rice slurry enzymolysis solution, and the fermentation conditions are: fermentation temperature 35 °C, fermentation time 36 hours, and the pH during fermentation is controlled between 6 and 6.5. After fermentation, a rice fermentation solution is obtained;

[0039] (3) The fermented rice fermentation solution is concentrated 10 times by reverse osmosis technology to efficiently enrich the active substances. After heating and sterilization and cooling and filtration, the concentrated rice fermentation solution, that is, the filtrate of yeast / rice fermentation product, is obtained.

[0040] Example 1

[0041] The anti-glycation and antioxidant composition described in this example comprises the following components in parts by weight: 0.2 part of carnosine hydrochloride, 6.5 parts of algal extract, and 3.5 parts of yeast / rice fermentation filtrate.

[0042] Example 2

[0043] The anti-glycation and antioxidant composition described in this example comprises the following components in parts by weight: 0.5 part of carnosine hydrochloride, 2.5 parts of algal extract, and 7.2 parts of yeast / rice fermentation filtrate.

[0044] Example 3

[0045] The anti-glycation and antioxidant composition described in this example comprises the following components in parts by weight: 0.8 part of carnosine hydrochloride, 4.8 parts of algal extract, and 4.6 parts of yeast / rice fermentation filtrate.

[0046] Example 4

[0047] The anti-glycation and antioxidant composition described in this example comprises the following components in parts by weight: 0.6 part of carnosine hydrochloride, 3.1 parts of algal extract, and 6.5 parts of yeast / rice fermentation filtrate.

[0048] Example 5

[0049] The anti-glycation and antioxidant composition described in this example comprises the following components in parts by weight: 1.0 part of carnosine hydrochloride, 7.5 parts of algal extract, and 1.7 parts of yeast / rice fermentation filtrate.

[0050] Examples 6 to 10

[0051] Examples 6 to 10 are the anti-aging cream described in the present invention, and its formulation is shown in Table 1; among them, the anti-aging creams described in Examples 6 to 10 respectively contain the anti-glycation and antioxidant compositions described in Examples 1 to 5.

[0052] Table 1 Formulation table of Examples 6 to 10 (mass percentage %)

[0053]

[0054]

[0055] The preparation method of the anti-aging cream described in Examples 6 to 10 above comprises the following steps:

[0056] (1) Add the chelating agent, humectant, thickener, and skin conditioner to deionized water, heat to 85 °C, homogenize and stir for 5 min to obtain a mixed solution A;

[0057] (2) Heat the emulsifier and emollient to 90 °C. After they are completely dissolved, add them to the mixed solution A, homogenize and stir for 5 min to obtain the mixed solution B;

[0058] (3) Cool the mixed solution B to 50 °C, add the anti-glycation and antioxidant composition (obtained by mixing decarboxylated carnosine hydrochloride, algal extract and yeast / rice fermentation product filtrate) and preservative, stir for 15 min, and filter to obtain the anti-aging cream.

[0059] Comparative Examples 1-5

[0060] Comparative Examples 1-5 are the anti-aging creams described in the present invention, and their formulations are shown in Table 2. Their preparation methods are the same as those of Example 9.

[0061] Table 2 Formulation table of Comparative Examples 1-5 (mass percentage %)

[0062]

[0063] Performance test

[0064] (1) Anti-glycation performance test

[0065] The glycation reaction occurs between reducing sugars and matrix proteins inside the skin and is one of the important mechanisms of skin aging. Non-enzymatic glycation is a series of complex non-enzymatic reactions. Proteins and reducing sugars (such as glucose) undergo non-enzymatic reactions in the body to form early glycation products, and then through processes such as oxidation, rearrangement, and crosslinking, finally form irreversible advanced glycation end products (AGEs), causing skin damage and aging. Therefore, substances that can effectively scavenge AGEs have significant anti-aging effects.

[0066] Test principle: AGEs can fluoresce spontaneously. By detecting the fluorescence intensity at a specific wavelength, the anti-glycation effects of different samples can be obtained.

[0067] The specific test steps are as follows:

[0068] (1) Bovine serum albumin (BSA)-glucose modeling: React a 0.05 mol / L phosphate buffered saline solution (PBS, pH = 7.4), an 8 mg / mL BSA solution, and a 0.2 mol / L glucose solution at 60 °C in the dark for 40 h to obtain a BSA-glucose glycation model solution (where the concentrations of PBS, bovine serum albumin, and glucose are the final concentrations in the mixed solution);

[0069] (2) Sampling: Accurately weigh 200 mg of different cream samples (i.e., the test samples) obtained from each example and comparative example, mix them evenly with 300 μL of the BSA-glucose glycation model solution, and react in the dark for 0.5 - 1 h;

[0070] (3) Detection of sample fluorescence: The fluorescence derived from AGEs can be detected by phosphate buffered saline under the conditions of an excitation wavelength of 370 nm and an emission wavelength of 440 nm;

[0071] (4) Data analysis: AGEs inhibition rate (%) = [1 - (A - B) / (C - D)] × 100%

[0072] Where: A - Fluorescence intensity of the BSA - glucose glycation model solution added with the test sample;

[0073] B - Fluorescence intensity of the ultrapure aqueous solution added with the test sample, that is, replacing 300 μL of the BSA - glucose glycation model solution in step (2) with 300 μL of ultrapure water;

[0074] C - Fluorescence intensity of the BSA - glucose glycation model solution without the test sample; that is, replacing 200 mg of different cream samples in step (2) with 200 mL of ultrapure water;

[0075] D - Fluorescence intensity of ultrapure water without the test sample and without the BSA - glucose glycation model solution.

[0076] Take the average of three measurements, and the test results are shown in Table 3 below:

[0077] Table 3 Anti - glycation effects of different cream samples

[0078] Project Example 6 Example 7 Example 8 Example 9 Example 10 AGEs inhibition rate (%) 70.7 75.3 80.5 96.1 82.2 Project Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 AGEs inhibition rate (%) 34.3 34.4 34.2 16.4 16.3

[0079] From the anti - glycation effect data of Examples 6 - 10 in Table 3, it can be seen that only when the three active ingredients of carnosine hydrochloride, algal extract, and yeast / rice fermentation product filtrate are added to the cream simultaneously and the three ratios are within a certain range, can the cream exert the maximum anti - glycation effect. From the anti - glycation effect data of Comparative Examples 1 - 5 in Table 3, it can be seen that when any one (two) of the active ingredients is not added, the anti - glycation effect of the cream will be greatly reduced. The above results show that carnosine hydrochloride, algal extract, and yeast / rice fermentation product filtrate can act synergistically to significantly improve the anti - glycation performance of the composition.

[0080] (2) Antioxidant performance test

[0081] Test principle: The DPPH method is used to test the scavenging effect of the anti - glycation and antioxidant composition of the present invention on free radicals. DPPH, also known as 1,1 - diphenyl - 2 - picrylhydrazyl, has a single electron and has a strong absorption at 517 nm, and its anhydrous ethanol solution is purple. When there is a free radical scavenger, due to the pairing of its single electron, its absorption gradually disappears, and the degree of fading is quantitatively related to the number of electrons it accepts. Therefore, a UV - visible absorption spectrometer can be used for rapid quantitative analysis.

[0082] Test method for measuring DPPH free radical scavenging rate: Take 2 g of each anti-aging cream prepared in Examples 6-10 and Comparative Examples 1-5, and mix it with 8 mL of 1×10 -4 mol / L DPPH / ethanol solution. After shaking evenly, react in the dark for 30 min (25 °C). Then place the mixture in a centrifuge and centrifuge at 7000 rpm for 5 min. Take the supernatant after centrifugation and measure the absorbance A1 at a wavelength of 517 nm; Take 2 mL of absolute ethanol and perform the same operation to measure the absorbance A2; Take 2 mL of deionized water and perform the same operation to measure the absorbance A3. Set 3 parallel experiments in each group to calculate the average value.

[0083] Calculate the free radical scavenging ability of the antioxidant and anti-glycation compositions in different groups according to the DPPH scavenging rate formula. DPPH scavenging rate = [1 - (A1 - A2) / A3] × 100%. The DPPH scavenging rate test results are shown in Table 4.

[0084] Table 4 DPPH scavenging rate test results of different cream samples

[0085]

[0086] From the DPPH scavenging rate data in Examples 6-10 and Comparative Examples 1-5 in Table 4, it can be seen that only when the three active ingredients of decarboxylated carnosine hydrochloride, algal extract, and yeast / rice fermentation product filtrate are added to the cream at the same time and in a reasonable proportion, can the cream maximize the DPPH free radical scavenging effect; when any one (two) of the active ingredients is not added, the DPPH free radical scavenging effect of the cream will be greatly reduced.

[0087] (3) Wrinkle reduction and anti-aging efficacy test

[0088] Test method: Select 300 healthy female volunteers aged 40-55 years old, divide them into 10 groups, with 30 people in each group, and apply the creams of Examples 6-10 and Comparative Examples 1-5 to the healthy volunteers in each group. The usage method is to wash the face with clear water every morning and evening, then apply it on the face once, gently press until absorbed, and use it continuously for 28 days. During the test period, the volunteers are not allowed to use any other products. Observe the facial wrinkles. If the skin becomes significantly firm and the wrinkles are significantly reduced, it is considered effective; if the facial skin becomes significantly firm and the wrinkles are reduced, it is considered effective; if there is no improvement in the facial skin, it is considered ineffective. The wrinkle reduction effect is shown in Table 5.

[0089] Table 5 Wrinkle reduction and anti-aging effect test of the creams prepared in Examples 6-10 and Comparative Examples 1-5

[0090]

[0091] As can be seen from Table 5, Comparative Example 1 compared with Example 9 shows that when decarboxylated carnosine hydrochloride is not added to the facial cream, the number of effective cases decreased from 25 people (83%) to 2 people (6%) after continuous use for 28 days, indicating that decarboxylated carnosine hydrochloride is crucial for the anti-aging effect of the facial cream; at the same time, Comparative Example 2 compared with Example 9 shows that when algae extract is not added to the facial cream, the number of effective cases decreased from 25 people (83%) to 2 people (6%), indicating that algae extract is also extremely important for the anti-aging effect of the facial cream; Comparative Example 3 compared with Example 9 shows that when yeast / rice fermentation product filtrate is not added to the facial cream, the number of effective cases decreased from 25 people (83%) to 2 people (6%).

[0092] In addition, Examples 6-10 and Comparative Examples 1-6 show that only when the three active ingredients of decarboxylated carnosine hydrochloride, algae extract and yeast / rice fermentation product filtrate are added to the facial cream at the same time and in an optimized ratio, can the facial cream exert the maximum anti-aging effect. When any one (two) of these active ingredients is not added, the anti-aging effect of the facial cream will be greatly reduced. This is because the three active ingredients of decarboxylated carnosine hydrochloride, algae extract and yeast / rice fermentation product filtrate in a reasonable ratio have excellent anti-glycation and antioxidant effects, and can exert the best anti-wrinkle and anti-aging effect to the greatest extent. In summary, the composition described in the present invention has dual effects of anti-glycation and antioxidant, and can significantly improve skin aging problems caused by oxidation and glycation.

[0093] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art without departing from the scope of the present invention should be within the protection scope of the present invention.

Claims

1. An anti-glycation and antioxidant composition, characterized in that, It comprises the following components in parts by weight: 0.1 to 1.5 parts of decarboxylated carnosine hydrochloride, 0.2 to 10.4 parts of algal extract, and 0.1 to 12.5 parts of yeast / rice fermentation product filtrate; The algal extract is the gonyaulax microalgae extract product of Guangzhou Nuoran Biotechnology Co., Ltd.; The yeast / rice fermentation product filtrate is prepared according to the following method: (1) Rice powder is added with water and heated, and stirred to become a 30 wt% rice slurry. β-glucanase is added to the rice slurry, and the addition amount of the enzyme is 0.15% of the mass of the rice slurry, and the enzyme activity is 200 U / g, and enzymatic hydrolysis is carried out to obtain a rice slurry enzymatic hydrolysate; (2) The yeast strain saccharomyces veronae is inoculated into the rice slurry enzymatic hydrolysate, and the fermentation conditions are: fermentation temperature 35 °C, fermentation time 36 hours, and the pH during the fermentation process is controlled between 6 and 6.

5. After fermentation, a rice fermentation broth is obtained; (3) The fermented rice fermentation broth is concentrated 10 times by reverse osmosis technology to efficiently enrich active substances, and after heating and sterilization and cooling and filtration, the concentrated rice fermentation broth is obtained, that is, the yeast / rice fermentation product filtrate.

2. The anti-glycation and antioxidant composition according to claim 1, wherein The anti-glycation and antioxidant composition comprises the following components in parts by weight: 0.2 to 1.2 parts of decarboxylated carnosine hydrochloride, 0.4 to 8.2 parts of algal extract, and 1.0 to 10.4 parts of yeast / rice fermentation product filtrate.

3. The anti-glycation and antioxidant composition according to claim 1, wherein The anti-glycation and antioxidant composition comprises the following components in parts by weight: 0.4 to 1.0 parts of decarboxylated carnosine hydrochloride, 1.0 to 6.0 parts of algal extract, and 2.0 to 9.0 parts of yeast / rice fermentation product filtrate.

4. The anti-glycation and antioxidant composition according to claim 1, wherein The anti-glycation and antioxidant composition comprises the following components in parts by weight: 0.5 to 1.0 parts of decarboxylated carnosine hydrochloride, 2.1 to 4.5 parts of algal extract, and 5.0 to 7.0 parts of yeast / rice fermentation product filtrate.

5. An anti-aging cream, characterized in that, The anti-aging cream contains the anti-glycation and antioxidant composition according to any one of claims 1 to 4.

6. The anti-aging cream according to claim 5, characterized in that, The content of the anti-glycation and antioxidant composition in the anti-aging cream is 0.4 to 24.4 wt%.

7. The anti-aging cream according to claim 5, wherein The anti-aging cream further contains at least one of a chelating agent, a humectant, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative.

8. The anti-aging cream according to claim 7, characterized in that, The chelating agent is disodium EDTA; The humectant contains at least one of glycerol, sodium hyaluronate, 1,3-butanediol, trehalose, honey, inositol, urea, panthenol, and betaine; The skin conditioner contains at least one of ceramide, pantothenic acid, and p-hydroxyacetophenone; The emollient contains at least one of squalene, jojoba seed oil, castor oil, shea butter, caprylic / capric triglyceride, cetearyl alcohol, beeswax, and shea fruit butter; The emulsifier contains at least one of lecithin, lanolin, lanolin alcohol, cetearyl alcohol / cetearyl glucoside, and glyceryl stearate; The thickener contains at least one of xanthan gum, carbomer, modified starch, and high molecular polymer; The preservative contains at least one of phenoxyethanol, ethylhexylglycerin, and pentylene glycol.

9. The anti-aging cream according to claim 7, characterized in that, Comprising the following components in parts by weight: 0.4 to 24.4 parts of an anti-glycation and antioxidant composition, 0.01 to 0.04 parts of a chelating agent, 7.5 to 17 parts of a humectant, 0.5 to 3 parts of a skin conditioner, 2 to 7 parts of an emollient, 1.2 to 10 parts of an emulsifier, 0.1 to 2.0 parts of a thickener, and 0.1 to 0.5 parts of a preservative.

10. A method for preparing the anti-aging cream according to claim 9, characterized in that, Comprising the following steps: (1) Add the chelating agent, humectant, thickener, and skin conditioner to deionized water, heat to 80 to 90 °C, homogenize and stir for 3 to 5 min to obtain a mixed solution A; (2) Heat the emulsifier and emollient to 80 to 90 °C, and after they are completely dissolved, add them to the mixed solution A, homogenize and stir for 3 to 5 min to obtain a mixed solution B; (3) Cool the mixed solution B to 40 to 50 °C, add the anti-glycation and antioxidant composition and the preservative, stir for 5 to 15 min, and filter to obtain the anti-aging cream.

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