Anti-shedding hair tonic and method for preparing the same

Abstract

This invention discloses a hair growth tonic for preventing hair loss and strengthening hair, and its preparation method, belonging to the field of cosmetic formulations. The components of this invention work synergistically to improve the scalp environment and reduce inflammation and bacteria. The raw material ratio of this invention is reasonable, and the use of microemulsion technology allows the active ingredients to deeply penetrate the scalp and hair follicles, improving the hair follicle growth environment, promoting hair growth and increasing hair volume, and alleviating symptoms of androgenetic alopecia.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic products, specifically relating to a hair growth liquid for preventing hair loss and strengthening hair, and its preparation method. Background Technology

[0002] Hair plays an important role in the skin of mammals, such as protecting the body, maintaining body temperature and sensation, and also has a cosmetic effect in humans.

[0003] Currently, only two drugs are approved for the treatment of hair loss: finasteride and minoxidil.

[0004] In the treatment of hair loss, commonly used Western medicines include minoxidil and finasteride. However, both drugs are prone to relapse after discontinuation and can cause side effects such as skin irritation, scalp itching, hirsutism on the face and hands, dizziness, anorexia, and tachycardia. Traditional Chinese medicine (TCM) treatment for hair loss also faces some challenges: TCM syndrome differentiation is complex, and there is no unified standard for clinical diagnosis; clinical efficacy in hair regrowth has not seen significant breakthroughs, especially in treating long-term hair follicles; furthermore, most research focuses on summarizing clinical experience, lacking in-depth exploration through modern medical experiments, and many prescriptions are only suitable for patients with specific constitutions and conditions, exhibiting strong limitations and low general applicability. Additionally, while hair transplantation has some therapeutic effect, its large surgical trauma, high cost, and the need for multiple surgeries limit its clinical application; the effects of non-drug treatments such as acupuncture are also unsatisfactory.

[0005] Compared to medicines and surgery, people are more compliant with daily hair care products. Currently, there are many shampoos on the market that claim to prevent hair loss and promote hair growth, but most of these products do not perform well in practice.

[0006] Shiseido, a Japanese company, once launched Adenovital shampoo, whose main ingredient is adenosine. This ingredient is believed to have a hair-growth effect similar to minoxidil, and numerous medical papers support its hair-growth capabilities. However, adenosine has poor solubility in water systems with a pH of 5-7, which limits its use.

[0007] Studies have found that thinning hair and hair loss are mainly caused by the following factors: First, the deterioration of the scalp's ecological environment leads to a disorder in the scalp environment, causing hair follicles to loosen or become blocked; second, metabolic disorders in the body stimulate excessive secretion of sebaceous glands, resulting in a large amount of oil compressing the hair roots, obstructing the blood and nutrient supply needed by the hair, and causing seborrheic alopecia; third, a lack of trace elements leads to malnutrition of hair follicles, making it difficult for hair to grow.

[0008] Therefore, a hair growth serum that can improve the scalp environment, activate hair follicle tissue, and promote hair follicle repair is needed to solve this problem. Summary of the Invention

[0009] This invention provides a hair growth liquid for preventing hair loss and strengthening hair, and its preparation method, which can improve the scalp environment, activate hair follicle tissue, and promote hair follicle repair.

[0010] To achieve the above objectives, the present invention adopts the following technical solution:

[0011] A hair loss prevention and hair growth tonic contains the following components by weight:

[0012] Adenosine 1-5

[0013] Sodium PCA 0.6-1.2

[0014] Water-soluble Q10 compound ceramide 2-6

[0015] The water-soluble Q10 composite ceramide contains the following components:

[0016]

[0017] The preparation method of the water-soluble Q10 composite ceramide includes the following steps:

[0018] (1) Mix and dissolve coenzyme Q10, ceramide AP, ceramide NP, ceramide NS, soybean lecithin, and horse fat;

[0019] (2) After stirring evenly, add glycerin, propylene glycol and water; and stir while adding, and after mixing evenly, the initial emulsion is obtained.

[0020] (3) Homogenize the primary emulsion to obtain water-soluble Q10 composite ceramide.

[0021] It also includes the following components:

[0022]

[0023]

[0024] The aforementioned method for preparing a hair regrowth solution for hair loss includes the following steps:

[0025] (1) Take the extracts of Platycladus orientalis, Polygonum multiflorum, Eclipta prostrata, Sapindus mukorossi, ginsenosides, dipropylene glycol, sodium PCA and water; heat to ≥85℃, and after complete dissolution, cool down to at least ≤40℃;

[0026] (2) After cooling, add water-soluble Q10 compound ceramide, compound amino acids, and 1,2-hexanediol, and stir again until well mixed to obtain a hair growth liquid that prevents hair loss and strengthens hair.

[0027] The present invention also provides the application of the aforementioned anti-hair loss and hair-strengthening liquid in the preparation of cosmetics; specifically, its application as a composition to stimulate hair growth.

[0028] In particular, the cosmetic is a rinse-off or leave-on cosmetic.

[0029] In particular, the cosmetic contains a medically acceptable carrier.

[0030] In particular, the cosmetic is selected from shampoo, hair conditioner, hair mask, hair oil, hairspray, pomade, and eyebrow care liquid.

[0031] In the present invention:

[0032] Platycladus orientalis is referred to as Biota orientalis.

[0033] Pleuropterus multiflorus is referred to as Fallopia multiflora.

[0034] Eclipta prostrata is referred to as Eclipta prostrata.

[0035] Sapindus saponaria is referred to as Sapindus mukorossi.

[0036] In the present invention, through the compounding of adenosine, sodium PCA, and water-soluble Q10 complex ceramide, the content of keratinocyte growth factor and vascular endothelial growth factor in the corresponding part is increased, and the level of dihydrotestosterone is reduced, thereby promoting the synthesis of microvessels in the head, activating the proliferation of hair follicle stem cells, and promoting the increase in the number of hair follicles.

[0037] The comprehensive addition of extracts of Biota orientalis, Fallopia multiflora, Eclipta prostrata, and Sapindus mukorossi can achieve the effects of antibacterial and anti-inflammatory, promoting blood circulation to remove blood stasis, improving folliculitis, and inhibiting the 5α-R reduction reaction.

[0038] The additionally added ginsenosides and compound amino acids can further enhance the effects.

[0039] Dipropylene glycol and water are mainly used as solvents; in addition to being a solvent, 1,2-hexanediol is also used as a humectant and preservative.

[0040] The main effect of adenosine on hair is to promote the proliferation and differentiation of hair follicle cells, thereby increasing the quantity and quality of hair; it can also inhibit the activity of 5α-reductase, reduce the damage of androgens to hair follicles, and prevent hair loss.

[0041] Sodium PCA is a natural moisturizing factor present in the skin, which can absorb and lock in moisture, keep the skin and hair moisturized; it can also regulate the skin's pH balance, inhibit the growth of bacteria, and reduce the production of dandruff.

[0042] Coenzyme Q10 can protect hair follicle cells from free radical damage, delay hair follicle aging, and promote hair growth. Coenzyme Q10 can also stimulate the expression of angiogenesis factors in hair follicles, increase blood supply to hair follicles, and improve the nutritional status of hair.

[0043] Ceramides can prevent moisture loss and maintain the elasticity and shine of skin and hair; they can also regulate cell signaling pathways, affecting the cell cycle and apoptosis, thereby controlling hair growth and renewal.

[0044] Ginsenosides work on hair primarily by activating hair follicle cells and promoting blood circulation, thereby reviving hair follicles with declining hair growth capacity and replenishing nutrients to nourish, promote, and darken hair.

[0045] The main function of complex amino acids for hair is to provide the nutrients needed for hair structure and function, enhance hair strength and toughness, and prevent breakage and split ends; it can also repair damaged hair surface and improve hair shine and smoothness.

[0046] Arborvitae extract (arborvitae extract solution) can activate hair matrix cells and promote blood circulation, reviving hair follicles with declining hair growth ability and replenishing nutrients after promoting blood circulation, thus playing a role in nourishing and promoting hair growth.

[0047] The main effects of Polygonum multiflorum extract on hair are through nourishing the liver and kidneys, improving the nutritional status of hair follicles, promoting the synthesis and secretion of melanin, and making hair black and shiny; it can also inhibit the activity of 5α-reductase, reduce the damage of androgens to hair follicles, and prevent hair loss.

[0048] The main benefits of Eclipta prostrata extract for hair are clearing inflammation on the scalp, improving dandruff and itching; it can also promote microcirculation on the scalp, increase blood supply to hair follicles, and provide hair with sufficient oxygen and nutrients.

[0049] Soapberry extract has cleansing, oil-removing, and antibacterial properties. Its effects on hair primarily come from removing oil and dirt from the scalp, reducing clogged pores and bacterial infections, and preventing dandruff and acne. It can also balance scalp oil and moisture secretion, keeping the scalp clean and comfortable.

[0050] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0051] The components of this invention work synergistically to improve the scalp environment and reduce inflammation and bacteria. The raw material ratio of this invention is reasonable, and the use of microemulsion formulation technology allows the active ingredients to penetrate deep into the scalp hair follicles, improve the hair follicle growth environment, promote hair growth and increase hair volume, and alleviate the symptoms of androgenetic alopecia. Attached Figure Description

[0052] Figure 1 This is a before-and-after comparison image of one of the human hair growth experiments.

[0053] Figure 2 A schematic diagram of the area photographed for a human hair growth experiment. Detailed Implementation

[0054] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terminology used in the embodiments is for describing specific implementation schemes and does not constitute a limitation on the scope of protection of the present invention.

[0055] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Percentages and parts are by weight.

[0056] Some of the raw materials used in this invention are sourced from the following sources:

[0057] Adenosine: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0058] Sodium PCA: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0059] Coenzyme Q10: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0060] Ceramide AP: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0061] Ceramide NP: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0062] Ceramide NS: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0063] Soy lecithin: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0064] Horse fat: Guangzhou Jinbaolai Biotechnology Co., Ltd.

[0065] Glycerin: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0066] Propylene glycol: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0067] Dipropylene glycol: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0068] Ginsenosides: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0069] 1,2-Hexanediol: Guangzhou Meiyi Biotechnology Co., Ltd.

[0070] Complex Amino Acids: Shanghai Aladdin Biochemical Technology Co., Ltd.

[0071] Arborvitae extract: Guangzhou Meiyi Biotechnology Co., Ltd.

[0072] Polygonum multiflorum extract: Guangzhou Meiyi Biotechnology Co., Ltd.

[0073] Eclipta prostrata extract: Guangzhou Meiyi Biotechnology Co., Ltd.

[0074] Sapindus mukorossi extract: Guangzhou Meiyi Biotechnology Co., Ltd.

[0075] Complex Amino Acid A: Bailande Biochemical (Haimen) Co., Ltd.

[0076] Complex Amino Acid B: Bailande Biochemical (Haimen) Co., Ltd.

[0077] Complex Amino Acid C: Bailande Biochemical (Haimen) Co., Ltd.

[0078] The preparation of water-soluble Q10 complex ceramide includes the following steps:

[0079] (1) Weigh the raw materials according to the mass fractions and serial numbers shown in Table 1.

[0080] (2) Dissolve coenzyme Q10, ceramide AP, ceramide NP, ceramide NS, soybean lecithin, and horse fat in a constant temperature water bath at 75°C and stir for 15 minutes.

[0081] (3) Add glycerin, propylene glycol and water while stirring. After mixing quickly, the initial emulsion is obtained.

[0082] (4) The primary emulsion was homogenized in a high-pressure homogenizer at 80 MPa for 3 cycles to obtain a nano-liposome-like water-soluble Q10 composite ceramide.

[0083] (5) Store in a sealed container.

[0084] Table 1. Preparation of water-soluble Q10 composite ceramides

[0085] Component content / serial number 1 2 3 4 5 Coenzyme Q10 2 1 0.5 0.2 - Ceramide AP 0.5 0.3 0.2 0.2 0.2 Ceramide NP 0.5 1 1 1 1 Ceramide NS 0.5 0.2 0.3 0.3 0.3 Soy lecithin 2 6 4 3 4 Horse fat 8 3 5 6 5 glycerin 10 30 20 20 20 Propylene glycol 30 10 20 20 20 water Add to 100 Add to 100 Add to 100 Add to 100 Add to 100

[0086] The units in Table 1 are parts by mass, and "-" indicates that no corresponding addition was made.

[0087] Sample stability test

[0088] 1. Heat resistance test: The constant temperature incubator is adjusted to 48℃. Three samples of each example are placed in a transparent glass bottle, with a sample volume of 20ml / bottle. After sealing, the bottle is placed in the constant temperature incubator. After three months, the bottle is taken out, allowed to return to room temperature, and the appearance changes are observed.

[0089] 2. Cold resistance test: The constant temperature incubator was adjusted to 4℃. Three samples of each example were placed in a transparent glass bottle, with a sample volume of 20ml / bottle. After sealing, the bottles were placed in the constant temperature incubator. After three months, the bottles were taken out, allowed to return to room temperature, and the appearance changes were observed.

[0090] 3. Room temperature test: Take three samples of each of the above-prepared samples and put them into a transparent glass bottle with a sample volume of 20ml / bottle. After sealing, place the bottle at room temperature for 6 months and observe the changes in appearance.

[0091] Samples 1-4 were all orange semi-transparent liquids; sample 5 was a yellow semi-transparent liquid.

[0092] No precipitation or sedimentation was observed in the heat resistance test, room temperature test, or cold resistance test, and the original appearance was maintained.

[0093] The preparation of a hair growth serum that prevents hair loss and strengthens hair includes the following steps:

[0094] (1) Weigh the raw materials according to the mass fractions and serial numbers shown in Tables 2-1 and 2-2.

[0095] (2) Mix the raw materials of phase A in Tables 2-1 and 2-2 and heat them to 85 degrees Celsius until they are completely dissolved, then cool them down to 40 degrees Celsius.

[0096] (3) After adding the raw materials of phase B in Tables 2-1 and 2-2, stir again until homogeneous to obtain a hair growth liquid that prevents hair loss and strengthens hair.

[0097] Table 2-1 Preparation of hair growth liquid for preventing hair loss and strengthening hair

[0098]

[0099] Table 2-2 Preparation of hair growth liquid for preventing hair loss and strengthening hair

[0100]

[0101] The units in Tables 2-1 and 2-2 are parts by mass, and "-" indicates that no corresponding addition was made.

[0102] Sample stability test

[0103] 1. Heat resistance test: The constant temperature incubator is adjusted to 48℃. Three samples of each example are placed in a transparent glass bottle, with a sample volume of 20ml / bottle. After sealing, the bottle is placed in the constant temperature incubator. After three months, the bottle is taken out, allowed to return to room temperature, and the appearance changes are observed.

[0104] 2. Cold resistance test: The constant temperature incubator was adjusted to 4℃. Three samples of each example were placed in a transparent glass bottle, with a sample volume of 20ml / bottle. After sealing, the bottles were placed in the constant temperature incubator. After three months, the bottles were taken out, allowed to return to room temperature, and the appearance changes were observed.

[0105] 3. Room temperature test: Take three samples of each of the above-prepared samples and put them into a transparent glass bottle with a sample volume of 20ml / bottle. After sealing, place the bottle at room temperature for 6 months and observe the changes in appearance.

[0106] Samples 6-10 and B are orange-brown semi-transparent liquids; samples A, C, and D are transparent liquids.

[0107] No precipitation or sedimentation was observed in the heat resistance test, room temperature test, or cold resistance test, and the original appearance was maintained.

[0108] Human skin patch test

[0109] The nine groups of hair growth liquids that prevent hair loss and strengthen hair growth were tested using human skin patch experiments in accordance with the "2022 Cosmetic Safety Technical Specifications".

[0110] Skin reactions were observed according to standard at 30 min (after the indentation disappeared), 24 h and 48 h, and the results were recorded (see Table 3).

[0111] Table 3 Results of Human Safety Tests

[0112] Serial Number 30min 24h 48h 6 Level 0, 30 people Level 0, 30 people Level 0, 30 people 7 Level 0, 30 people Level 0, 30 people Level 0, 30 people 8 Level 0, 30 people Level 0, 30 people Level 0, 30 people 9 Level 0, 30 people Level 0, 30 people Level 0, 30 people 10 Level 0, 30 people Level 0, 30 people Level 0, 30 people A Level 0, 30 people Level 0, 30 people Level 0, 30 people B Level 0, 30 people Level 0, 30 people Level 0, 30 people C Level 0, 30 people Level 0, 30 people Level 0, 30 people D Level 0, 30 people Level 0, 30 people Level 0, 30 people

[0113] Anti-inflammatory effect cell test

[0114] This study investigates the anti-inflammatory activity of dermal papilla cells in response to excessive production of inflammatory factors induced by androgens (DHT).

[0115] 1. Experimental reagents and consumables

[0116] MSCM culture medium

[0117] PBS buffer

[0118] DMSO

[0119] Dihydrotestosterone (DHT)

[0120] IL1β ELISA kit

[0121] IL-6 ELISA kit

[0122] Sterile ddH2O (Distillation-DistillationH2O).

[0123] 2. Methods and Steps

[0124] 1) Vaccination: at a dose of 1.1 × 10 4 Inoculate dermal papilla cells into 6-well plates at a seeding density of cells / well and incubate overnight in a CO2 incubator.

[0125] 2) DHT stimulation and administration: According to the test protocol shown in Table 4, when the cell seeding rate in the 6-well plate reached 50-60%, the drug was administered and the groups with DHT stimulation conditions were stimulated with DHT. After the drug was administered, the cells were incubated in a (37℃, CO2) incubator for 24h, 48h and 72h. For the groups that required incubation for 48h and 72h, the drug was administered and the medium was changed daily.

[0126] 3) Cell proliferation assay: After cell culture, discard the supernatant, add MTT working solution (0.5 mg / mL, freshly prepared), and incubate at 37°C in the dark for 4 hours. After incubation, discard the supernatant, add 150 μL LDMSO to each well, and read the OD value at 490 nm. Simultaneously, perform the above MTT assay after 48 hours and 72 hours.

[0127] 4) Calculation of relative cell viability: According to the formula, relative cell viability (%) = (sample well OD - zeroing well OD) / (solvent control well OD - zeroing well OD) * 100%.

[0128] 5) Statistical analysis of results:

[0129] The concentrations of IL-1β and IL-6 were detected using ELISA.

[0130] The t-test was used for comparisons between groups. All statistical analyses were two-tailed. P < 0.05 was considered statistically significant, and P < 0.01 was considered highly statistically significant.

[0131] 3. Experimental Results

[0132] The sample information and testing system are shown in Table 4, and the results are shown in Table 5.

[0133] Table 4 Anti-inflammatory test protocol

[0134]

[0135]

[0136] Table 5. Results of anti-inflammatory tests

[0137]

[0138] Antibacterial test

[0139] The antibacterial effect of the present invention was tested using Malassezia as a representative example.

[0140] According to "QBT 2738-2012(7.3) - Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products", the hair growth liquid for preventing hair loss and strengthening hair of the present invention was subjected to Malassezia antimicrobial test.

[0141] 1. Methods and Steps

[0142] Under aseptic conditions, the Malassezia suspension was diluted with PBS to a concentration of 1-9 × 10⁻⁶. 4 cfu / mL.

[0143] Place the samples into sterile test tubes and incubate at 20°C for 30 minutes. Add 0.1 mL of the test bacterial solution to a 5 mL sample tube and start timing.

[0144] After the set time (5 min), add 0.5 mL of the mixture of test bacteria and sample to 4.5 mL of neutralizing agent, mix well, neutralize for 10 min, then transfer the sample solution to culture medium and incubate for 48 h. Calculate the number of viable colonies. Repeat the above steps using PBS instead of the test sample as a control sample.

[0145] The neutralizing agent is prepared as follows: Take 20g of Tween-80, 10g of sodium thiosulfate, and 1000ml of PBS solvent, and mix them evenly.

[0146] The formula for calculating the antibacterial rate (%) is as follows:

[0147] Antibacterial rate (%) = (Ⅰ-Ⅱ / Ⅰ)×100

[0148] In the formula: Ⅰ represents the average colony count of the control sample; Ⅱ represents the average colony count of the test sample.

[0149] 2. Experimental Results

[0150] As shown in Table 6.

[0151] Table 6 Results of Malassezia antibacterial test

[0152]

[0153]

[0154] Animal hair growth test

[0155] Animal experiments were used to test the hair regrowth effect of the embodiments of the present invention.

[0156] 1. Methods and Steps

[0157] Sixty 12-week-old female mice were selected. After being housed separately for 10 days, the mice were dehaired using an experimental animal depilatory agent to remove hair from the corresponding areas on their backs, with a removal area of ​​2cm × 2cm. The skin of the mice after hair removal was pink and in the resting phase of the hair growth.

[0158] Fifty mice with no damage to the hair removal area were randomly divided into 10 groups.

[0159] The experimental procedure was as follows: no application was made to the hair removal area in the control group;

[0160] The remaining nine groups were coated with their respective samples;

[0161] After applying the product continuously for 28 days, the number of active hair follicles was counted.

[0162] 2. Experimental Results

[0163] As shown in Table 7.

[0164] Table 7 Results of the number of active hair follicles in mice

[0165] Group Number of active hair follicles (number / 400×) p-value Blank group 3.55±0.42 / 6 5.23±0.18 ＜0.05 7 5.32±0.23 ＜0.01 8 5.42±0.26 ＜0.01 9 5.33±0.28 ＜0.01 10 5.49±0.26 ＜0.01 A 4.21±0.38 ＜0.05 B 4.68±0.29 ＜0.05 C 4.34±0.26 ＜0.05 D 3.89±0.45 ＞0.05

[0166] Human hair growth test

[0167] (I) Experimental Basis

[0168] The efficacy of hair loss prevention refers to its ability to improve or reduce hair loss, which can be verified through human efficacy evaluation tests. The tests were conducted in accordance with "Chapter 8 Human Efficacy Evaluation Test Methods 6 Test Methods for Hair Loss Prevention Efficacy of Cosmetics" in the "Cosmetic Safety Technical Specifications" and "Chen Mingxuan, Zeng Yansheng, Fang Zhaohua. Preparation and Efficacy Study of an Anti-Hair Loss Shampoo and Anti-Hair Loss Essence [J]. Guangdong Chemical Industry, 2018, 045(021): 38-39."

[0169] (II) Experimental Objectives and Principles

[0170] Hair loss is a common scalp problem characterized by hair reduction. The hair growth cycle is mainly divided into the anagen (growth) phase, catagen (transitional) phase, and telogen (resting) phase. Anti-hair loss cosmetics primarily work by promoting the normal development of new hair, prolonging the anagen phase, and reducing hair loss rates, thereby preventing hair loss. Observing hair follicle changes using a dermoscopy and counting the number of lost hairs are common methods used in testing anti-hair loss cosmetics.

[0171] This experiment involved using shampoo and serum containing the test product 3-4 times per week. Hair loss was collected during each shampooing session, during the shampooing, testing, and recovery periods. The average number of hairs lost was calculated over two weeks, and dermoscopic observation was performed to elucidate the efficacy of the test product.

[0172] (III) Test Methods

[0173] 1. Experimental Design

[0174] 1.1 Subject Selection: 26 healthy subjects aged 20-60 years, regardless of gender, were selected. The inclusion, exclusion, and restriction criteria for subjects are as follows:

[0175] 1.1.1 Patients experiencing significant hair loss and mild thinning, who are not suffering from alopecia areata or androgenetic alopecia;

[0176] 1.1.2 Those who have not had any special hair treatments such as hair dyeing, perming, or styling within the past month;

[0177] 1.1.3 Those who have not used any products or medications with anti - hair - loss or hair - growth effects, and have not received hair transplantation treatment within the past week;

[0178] 1.1.4 Those whose test sites are participating in other clinical trials;

[0179] 1.1.5 During the trial, no hair care or styling measures can be taken, and no anti - hair - loss or hair - growth treatments can be received. Do not cut hair for 2 months and maintain the original lifestyle; <​​​​​​​​​​​​​​​​​​​​

[0187] 1.7 Dermoscopy for hair follicle examination: Subjects with severe hair loss (more than 100 hairs lost per wash) were selected. Areas of the scalp with significant hair loss were shaved, and scalp images were taken using a dermoscope. The same location was used for each image, followed by shaving and re-image taking until the experiment concluded.

[0188] 2. Main experimental materials

[0189] Using a dermal mirror to take photos of the area such as... Figure 2 As shown.

[0190] (iv) Test Results

[0191] 1. Data Statistics

[0192] A total of 26 subjects were recruited. All test data of the subjects conformed to a normal distribution. Paired t-tests were used to compare the data before and after the subjects (analyzed using Prism5 software).

[0193] 2. Test Results

[0194] As shown in Table 8.

[0195] Table 8 Results of Human Hair Growth Experiment

[0196]

[0197]

[0198] (v) Adverse reactions / events

[0199] No adverse reactions occurred during the entire testing process.

[0200] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. A hair growth tonic for preventing hair loss and strengthening hair, characterized in that, It contains the following components in parts by mass: Adenosine 1-5 Sodium PCA 0.6-1.2 Water-soluble Q10 compound ceramide 2-6 Platycladus orientalis extract 1.0-3.0 Polygonum multiflorum extract 0.5-1.5 Eclipta prostrata extract 0.5-1.5 Sapindus mukorossi extract 2.0-5.0; The water-soluble Q10 composite ceramide contains the following components: Coenzyme Q10 0.2-2 Ceramide AP 0.2-0.5 Ceramide NP 0.5-1 Ceramide NS 0.2-0.5 Soy lecithin 2-6 Horse fat 3-8 Glycerin 10-30 Propylene glycol 10-30.

2. The hair growth liquid for preventing hair loss and strengthening hair as described in claim 1, characterized in that, The preparation method of the water-soluble Q10 composite ceramide includes the following steps: (1) Mix and dissolve coenzyme Q10, ceramide AP, ceramide NP, ceramide NS, soybean lecithin, and horse fat; (2) After stirring evenly, add glycerin, propylene glycol and water; and stir while adding, and mix well to obtain the initial emulsion; (3) Homogenize the primary emulsion to obtain water-soluble Q10 composite ceramide.

3. The hair growth liquid for preventing hair loss and strengthening hair as described in claim 1, characterized in that, It also includes the following components: Dipropylene glycol 10-40.

4. The hair growth liquid for preventing hair loss and strengthening hair as described in claim 1, characterized in that, It also includes the following components: Ginsenosides 0.1-0.3%.

5. The hair growth liquid for preventing hair loss and strengthening hair as described in claim 1, characterized in that, It also includes the following components: 1,2-Hexanediol 1-3.

6. The hair growth liquid for preventing hair loss and strengthening hair as described in claim 1, characterized in that, It also includes the following components: Complex amino acids 1-5.

7. The method for preparing the hair growth liquid for preventing hair loss and strengthening hair as described in any one of claims 1-6, characterized in that, Includes the following steps: (1) Heat the raw materials except for water-soluble Q10 composite ceramide and water to ≥85℃, and after complete dissolution, cool down to at least ≤40℃; (2) After cooling, add water-soluble Q10 compound ceramide and stir again until well mixed to obtain a hair growth liquid that prevents hair loss and strengthens hair; If the formula contains compound amino acids and 1,2-hexanediol, then add them in step (2).

8. The use of the hair growth liquid for preventing hair loss and strengthening hair as described in any one of claims 1-6 in the preparation of cosmetics.

9. The application according to claim 8, characterized in that, The cosmetics mentioned are either rinse-off or leave-on cosmetics.

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