A composition for increasing dermal thickness, an anti-aging cosmetic, and a method of preparing the same

Abstract

The application belongs to the technical field of daily cosmetics, and discloses a composition for increasing dermal thickness, an anti-aging cosmetic and a preparation method thereof.The composition for increasing dermal thickness comprises the following active components in parts by weight: 0.0001-2.0 parts of hexapeptide-11, 0.01-5.0 parts of algal extract and 0.1-12 parts of yeast / rice fermentation product filtrate.The composition for increasing dermal thickness in a specific content is added to an anti-aging cosmetic base formula, and the prepared anti-aging cosmetic can directly act on dermal tissue and cells of the skin, rebuild dermal structure, and increase dermal thickness and density.In addition, the anti-aging cosmetic can effectively promote the synthesis of collagen, elastin and extracellular matrix components in the skin, and can supplement the skin's own filaggrin or bottom layer fibers to increase skin elasticity.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily cosmetics, and in particular relates to a composition for increasing dermal thickness, an anti-aging cosmetic and a preparation method thereof. Background Art

[0002] As we age, our bodies age. Skin aging is one of the most prominent manifestations of overall body aging, especially facial skin aging. Skin aging is closely related to the extracellular matrix (ECM) of the dermis. Collagen and elastin are important components of the extracellular matrix, providing toughness and elasticity to the skin. Fibroblasts are cells responsible for synthesizing the extracellular matrix and are located in the network formed by the extracellular matrix in the dermis. As the body ages, the number of fibroblasts in the human body decreases, and the collagen and elastin they secrete gradually decrease and break due to oxidation, losing their support for the epidermis, causing skin collapse. At the same time, the dermis gradually becomes thinner, skin elasticity deteriorates, and wrinkles form.

[0003] However, existing anti-aging products generally only address aging in the epidermis, rarely truly improving the dermis. While retinol-based substances can improve dermal aging, they are highly irritating, easily triggering adverse skin reactions and damaging the skin barrier. Therefore, there remains a need for an effective, gentle anti-aging product that addresses aging in the dermis. Summary of the Invention

[0004] In view of the fact that the anti-aging products in the prior art are either rarely able to truly improve the aging of the dermis or are highly irritating and can damage the skin barrier, the purpose of the present invention is to provide a safe and mild composition for increasing the thickness of the dermis, an anti-aging cosmetic and a preparation method thereof.

[0005] The technical solutions adopted in the present invention are as follows:

[0006] The present invention first provides a composition for increasing dermal thickness, comprising the following active ingredients in parts by weight:

[0007] 0.0001-2.0 parts hexapeptide-11, 0.01-5.0 parts algae extract, and 0.1-12 parts yeast / rice fermentation product. Furthermore, the composition for increasing dermal thickness comprises the following active ingredients, by weight: 0.001-2.0 parts hexapeptide-11, 0.5-5.0 parts algae extract, and 5-12 parts yeast / rice fermentation product.

[0008] Furthermore, in the composition for increasing dermal thickness, the mass ratio of hexapeptide-11, algae extract and yeast / rice fermentation product is (0.1-2):(0.8-5):(2-12).

[0009] The present invention further provides an anti-aging and anti-wrinkle cosmetic comprising the above-mentioned composition for increasing dermal thickness. The cosmetic includes makeup and skin care products such as creams, lotions, essences, facial masks, and eye creams.

[0010] The yeast / rice fermentation product filtrate is rich in various natural skin-nourishing ingredients (such as fruit acid, amino acids, choline, succinic acid, and nucleic acids), which can help rebuild the barrier function of the dermal matrix and replenish the skin's own filaggrin, or underlying fibers. Furthermore, the yeast / rice fermentation product filtrate has excellent skin affinity and permeability, promoting the absorption of subsequent active ingredients.

[0011] In one embodiment of the present invention, the yeast / rice fermentation product filtrate is obtained by fermenting rice slurry with yeast.

[0012] In a preferred embodiment of the present invention, the yeast / rice fermentation product filtrate is prepared by fermentation and enrichment processes using yeast Saccharomyces veronae.

[0013] The present invention provides a feasible method for preparing yeast / rice fermentation product filtrate, which is specifically:

[0014] (1) Rice powder is heated and stirred with water to obtain 20-40 wt% rice milk, β-glucanase is added to the rice milk, the amount of the enzyme added is 0.001%-0.5% of the rice milk mass, and the enzyme activity is 150-200 U / g, and enzymolysis is performed to obtain a rice milk enzymolyte;

[0015] (2) inoculating yeast into rice milk enzymatic hydrolysate, fermenting under the following conditions: fermentation temperature 20-40° C., fermentation time 24-48 hours, pH control of the fermentation process to between 6-6.5, and obtaining rice fermentation liquid after fermentation;

[0016] (3) The fermented rice broth is concentrated by reverse osmosis technology to efficiently enrich the active substances, and then sterilized by heating and cooled and filtered to obtain the concentrated rice broth, i.e., the yeast / rice fermentation product filtrate of the present invention.

[0017] Hexapeptide-11 is a synthetic peptide widely used in cosmetic anti-aging ingredients. It can act on dermal fibroblasts, promote the expression of related genes in the skin's extracellular matrix, thereby promoting the synthesis of collagen and elastin, increasing skin elasticity and firmness, and improving static wrinkles.

[0018] Algae extract contains a variety of active ingredients such as carbohydrates, proteins and amino acids (such as glutamic acid, aspartic acid, alanine, lysine).

[0019] In one embodiment of the present invention, the algae extract is golden microalgae. Golden microalgae is an algae extract (such as Platymonas S extract) that, when cultivated with gold salts, converts "mineral gold" into active "bio-gold" (abbreviated as "active gold"), enriching the algae cells with active ingredients such as polysaccharides, oligosaccharides, proteins, and amino acids. Compared to conventional algae extracts, algae extracts containing active gold exhibit superior performance in promoting skin renewal, anti-oxidation, and rebuilding dermal structure. Golden microalgae have a unique ability to bioaccumulate gold and gold compounds. Leveraging this characteristic, "mineral gold" can be converted into "active gold" that can gently repair skin, thereby reducing irritation and threats to the skin. Under defined and very specific environmental conditions, golden microalgae can bioaccumulate gold in their cytoplasm, where it is absorbed and provided in the matrix.

[0020] The present invention further provides an anti-aging cosmetic comprising the above-mentioned composition for increasing dermal thickness.

[0021] Preferably, the mass percentage of the composition for increasing dermal thickness in the anti-aging cosmetic is 0.1-20%. Without limitation, the mass percentage of the anti-aging and anti-wrinkle composition in the cosmetic includes but is not limited to 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, and 20%.

[0022] As a further preferred embodiment of the present invention, the anti-aging cosmetic further comprises at least one of a chelating agent, a moisturizer, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative.

[0023] As a further preferred embodiment of the present invention, the chelating agent is disodium EDTA; the moisturizing agent comprises at least one of glycerin, sodium hyaluronate, trehalose, honey, inositol, panthenol and betaine; the skin conditioning agent comprises at least one of ceramide, pantothenic acid and magnesium gluconate; the emollient comprises at least one of squalene, jojoba seed oil, beeswax, castor oil, avocado oil and avocado butter; the emulsifier comprises at least one of lecithin, lanolin, lanolin alcohol, sorbitan stearate and sorbitan olivate; the thickener comprises at least one of xanthan gum, carbomer, cetyl alcohol, sodium polyacrylate and tapioca starch; and the preservative is phenoxyethanol.

[0024] As a further preferred embodiment of the present invention, the anti-aging cosmetic comprises the composition for increasing dermal thickness, a chelating agent, a moisturizer, a thickener, a skin conditioner, an emulsifier, an emollient, and deionized water. In the anti-aging cosmetic, the cosmetic comprises the following components, calculated by mass percentage in the cosmetic:

[0025] Chelating agent 0.01-0.02%;

[0026] Moisturizer 0.5-10%;

[0027] Skin conditioner 0.01-0.05%;

[0028] Emollient 1-2%;

[0029] Emulsifier 0.02-10%;

[0030] Thickener 0.1-3.0%;

[0031] Preservative 0.1-1%;

[0032] Composition for increasing dermal thickness 0.1-20%.

[0033] In some optional embodiments of the present invention, the chelating agent is disodium EDTA; the moisturizing agent comprises at least one of glycerin, sodium hyaluronate, trehalose, honey, inositol, panthenol and betaine; the skin conditioning agent comprises at least one of ceramide, pantothenic acid and magnesium gluconate; the emollient comprises at least one of squalene, jojoba seed oil, beeswax, castor oil, avocado oil and avocado butter; the emulsifier comprises at least one of lecithin, lanolin, lanolin alcohol, sorbitan stearate and sorbitan olivate; the thickener comprises at least one of xanthan gum, carbomer, cetyl alcohol, sodium polyacrylate and tapioca starch; and the preservative is phenoxyethanol.

[0034] In some optional embodiments of the present invention, the chelating agent is disodium EDTA; the humectant is a mixture of glycerin, sodium hyaluronate, inositol, and panthenol; the skin conditioning agent is magnesium gluconate; the emollient is a mixture of jojoba seed oil and beeswax; the emulsifier is sorbitan olivate; the thickener is cetyl alcohol; and the preservative is phenoxyethanol.

[0035] The anti-aging cosmetic can increase the thickness and density of the dermis.

[0036] The present invention provides a method for preparing an anti-aging cosmetic comprising the composition for increasing dermal thickness, a chelating agent, a moisturizer, a thickener, a skin conditioner, an emulsifier, and an emollient, comprising the following steps:

[0037] (1) adding a chelating agent, a moisturizing agent, a thickener, and a skin conditioning agent to deionized water, heating and homogenizing, and stirring to obtain a mixed solution A;

[0038] (2) heating the emulsifier and the emollient until they are completely dissolved, adding them to the mixed solution A, adding the preservative, homogenizing, and stirring to obtain the mixed solution B;

[0039] (3) Cooling the mixed solution B, adding the composition for increasing dermis thickness, stirring, and filtering.

[0040] The present invention further provides a method for preparing an anti-aging cosmetic comprising the composition for increasing dermal thickness, a chelating agent, a moisturizer, a thickener, a skin conditioner, an emulsifier, and an emollient, comprising the following steps:

[0041] (1) Add a chelating agent, a moisturizing agent, a thickener, and a skin conditioning agent to deionized water, heat to 80-90° C., homogenize, and stir for 3-5 minutes to obtain a mixed solution A;

[0042] (2) Heat the emulsifier and emollient to 80-90°C, add them to the mixed solution A after they are completely dissolved, and add the preservative, homogenize and stir for 3-5 minutes to obtain the mixed solution B;

[0043] (3) Cooling the mixed solution B to 40-50° C., adding the composition for increasing dermal thickness, stirring for 5-15 minutes, and filtering to obtain the anti-aging cosmetic.

[0044] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0045] (1) The yeast / rice fermentation product filtrate of the present invention is not only rich in a variety of natural skin care ingredients, but also has excellent skin affinity and permeability, which can promote the absorption of the active ingredients hexapeptide-11 and algae extract in the dermis, and better exert the anti-aging effect of the active ingredients.

[0046] (2) The algae extract of the present invention contains a variety of active ingredients such as carbohydrates, proteins and amino acids (such as glutamic acid, aspartic acid, alanine, lysine), which can promote skin renewal from the source, rebuild the skin's dermal structure, and restore the skin to a healthy structure.

[0047] (3) The present invention contains hexapeptide-11, algae extract and yeast / rice fermentation product filtrate. The three active ingredients work synergistically with each other and can directly act on the dermis, repair the dermal extracellular matrix, rebuild the skin dermal structure, increase the thickness and density of the dermis, promote the synthesis of collagen, elastin, filaggrin and underlying fibers, and exert an anti-aging effect from the root. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 : A, B, C, D, E, and F in the figure are Dermlab Combo skin tomographic images before using the sample, and after using the cream in Example 3, Example 4, Example 5, Example 6, and Example 7 for 28 days. DETAILED DESCRIPTION

[0049] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0050] The following examples further illustrate various embodiments within the scope of the present invention. These examples are given for illustrative purposes only and are not intended to limit the present invention, as many variations are possible without departing from the spirit and scope of the present invention.

[0051] In the following examples,

[0052] Hexapeptide-11 was purchased from Wuhan Haishan Technology Co., Ltd.

[0053] The method for preparing the yeast / rice fermentation product filtrate is as follows:

[0054] (1) Rice powder from Akita County, Japan, was heated with water and stirred to obtain 30 wt% rice milk, β-glucanase was added to the rice milk in an amount of 0.15% of the mass of the rice milk and an enzyme activity of 200 U / g, and enzymolysis was performed to obtain a rice milk enzymolyzate;

[0055] (2) inoculating the yeast strain Saccharomyces veronae into the rice milk enzymatic hydrolysate, and fermenting under the following conditions: fermentation temperature of 35° C., fermentation time of 36 hours, and pH control of the fermentation process between 6 and 6.5, to obtain rice fermentation liquid;

[0056] (3) The fermented rice fermentation liquid is concentrated 10 times by reverse osmosis technology to efficiently enrich the active substances, and then sterilized by heating and cooled and filtered to obtain the concentrated rice fermentation liquid, i.e., the yeast / rice fermentation product filtrate of the present invention.

[0057] The algae extract was golden microalgae purchased from Weidan Biotechnology Co., Ltd. (obtained by culturing Platymonas S extract and gold salt).

[0058] Examples 1 to 7

[0059] Examples 1 to 7 are the anti-aging creams of the present invention, and their formulas are shown in Table 1.

[0060] Table 1 Formula table of Examples 6 to 12 (mass percentage %)

[0061]

[0062] The preparation method of the anti-aging cream described in Examples 1 to 7 comprises the following steps:

[0063] (1) Add a chelating agent, a moisturizing agent, a thickener, and a skin conditioning agent to deionized water, heat to 90° C., homogenize, and stir for 5 minutes to obtain a mixed solution A;

[0064] (2) Heat the emulsifier and emollient to 90°C, add them to the mixed solution A after they are completely dissolved, and add the preservative, homogenize and stir for 5 minutes to obtain the mixed solution B;

[0065] (3) Cooling the mixed solution B to 50° C., adding a composition for increasing dermal thickness (obtained by mixing hexapeptide-11, algae extract, and yeast / rice fermentation product filtrate), stirring for 15 minutes, and filtering to obtain the anti-aging cream.

[0066] Comparative Examples 1 to 5

[0067] Comparative Examples 1 to 5 are the anti-aging creams of the present invention. Except that the components of the composition for increasing dermal thickness are different from those in Example 3, the rest of the formulas are the same as those in Example 3. The preparation methods are also the same as those in Example 3. The formulas of the composition for increasing dermal thickness are shown in Table 2.

[0068] Table 2 Formula table of comparative examples 1 to 5 (mass percentage %)

[0069]

[0070] Performance Testing

[0071] (1) Stability assessment

[0072] Test samples: Examples 1 to 7 and Comparative Examples 1 to 5

[0073] Test Method: Heat resistance testing involves exposing the cream to 45±2°C for three months and observing its appearance, color, and odor. Cold resistance testing involves exposing the cream to -18±2°C for three months and observing its appearance, color, and odor. Heat and cold cycling testing involves exposing the cream to 45°C, room temperature, and -18°C for three cycles of 24 hours each, and observing its appearance, color, and odor. The test results are shown in Table 3.

[0074] Table 3 Stability test results

[0075]

[0076] As shown in Table 3, the anti-aging creams described in Examples 1-7 and Comparative Examples 1-4 exhibit excellent stability, maintaining their properties under high and low temperatures, as well as high and low temperature cycling conditions. However, the excessive amount of algae extract added to Comparative Example 5 resulted in an uneven white cream with stratification, and exhibited abnormal heat resistance and resistance to both heat and cold cycling.

[0077] (2) Safety evaluation of anti-aging creams

[0078] Test method: Human skin patch test is used to evaluate product safety.

[0079] Test substances: facial creams prepared in Examples 1 to 7 and Comparative Examples 1 to 5.

[0080] Subjects: A total of 30 subjects, 15 males and 15 females, aged 25 to 35 years, who met the volunteer inclusion criteria;

[0081] Spot test method: Use a qualified spot tester and a closed patch test method. Apply approximately 0.02-0.025g of the creams prepared in Examples 1-7 and Comparative Examples 1-5 to the spot tester. Apply the cream to the curved side of the subject's forearm with a special adhesive tape. Remove the test substance after 24 hours. Observe the skin reaction 30 minutes after the removal of the spot tester, after the indentation disappears. Record the results according to the skin reaction grading standards in the "Cosmetics Safety Technical Regulations 2015". Record the reaction results in Table 5 according to the adverse reaction grading standards below (Table 4).

[0082] Table 4: Grading criteria for adverse skin reactions

[0083]

[0084] Table 5 Results of human skin patch tests on the creams in Examples 1 to 7 and Comparative Examples 1 to 5

[0085]

[0086] As shown in Table 5, the cosmetic emulsions prepared in Examples 1-7 and Comparative Examples 1-4, when applied to the flexed side of the arm, all 30 subjects showed negative skin reactions, receiving a grade of 0. Based on the human skin patch test criteria in the "2015 Cosmetics Safety Technical Regulations," the cosmetics prepared in Examples 1-7 and Comparative Examples 1-4 showed no adverse reactions to human skin and passed the safety test. Comparative Example 5, however, failed the safety test, likely due to the excessive amount of algae extract added.

[0087] (3) In vitro efficacy experiments of golden microalgae

[0088] Transcriptomic analysis: Microfluidic RT-qPCR technology, a cross-disciplinary field between nanotechnology and RT-PCR gene analysis, was used to evaluate gene expression in normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF). NHEK and NHDF were seeded into 96-well plates at appropriate cell densities and incubated in a 37°C cell culture incubator until the cell confluence reached approximately 80%. Subsequently, serum-free cell culture medium (containing 0% and 1wt% gold microalgae) was added and incubated for 24 hours. Finally, microfluidic RT-qPCR was used to analyze the differences in the expression of 90 genes between the two cell lines. Each group was repeated three times, and the changes in gene expression were expressed as a percentage of the control group (pure cell culture medium without gold microalgae). The average value of each group was taken. The gene expression change rate of 1 wt% gold microalgae = (B-A) / A*100%, where A is the gene expression level after treatment with serum-free cell culture medium (containing 0% gold microalgae), and B is the gene expression level after treatment with serum-free cell culture medium (containing 1 wt% gold microalgae). The experimental results are shown in Table 6.

[0089] Table 6 Transcriptomic gene analysis test results of gold microalgae (change rate compared with the control group)

[0090]

[0091]

[0092] As shown in Table 6, 1% gold microalgae can not only effectively upregulate multiple genes such as filaggrin, epidermal growth factor, and glutaredoxin in keratinocytes, but also upregulate multiple genes such as collagen 1, elastin, hyaluronan synthase 2, and collagen 17 in fibroblasts, and downregulate matrix metalloproteinase-1 and matrix metalloproteinase-3, indicating that it has powerful efficacy and excellent regenerative activity, and can rebuild the structure of the epidermis and dermis.

[0093] (4) Anti-wrinkle and anti-aging efficacy test

[0094] Test method: 330 healthy female volunteers aged 40 to 55 were selected and divided into 11 groups, with 30 people in each group. The creams of Examples 1 to 7 and Comparative Examples 1 to 4 were used on the healthy volunteers in each group. The method of use is to use it for skin care in the morning and evening. Use the fingertips to take an amount of the test sample about the size of a soybean, apply it evenly to the facial skin, and gently massage until absorbed. Use it continuously for 28 days. During the test period, volunteers were not allowed to use any other products. The effects of skin elasticity improvement rate, firmness increase rate, dermis thickness and density increase rate are shown in Table 7. The dual MPA580s detect skin elasticity R2 and skin firmness Uf; the collagen probe DermlabCombo is used to capture facial images and detect and analyze the density and thickness of the dermis.

[0095] In Table 7, skin elasticity improvement rate (%) = (average skin elasticity of each group after using the example / comparative example cream - average skin elasticity of each group before use) / average skin elasticity of each group before use * 100%

[0096] Skin firmness increase rate (%) = (average skin firmness of each group after using the example / comparative example cream - average skin firmness of each group before use) / average skin elasticity of each group before use * 100%

[0097] Skin dermis thickness increase rate (%) = (average skin dermis thickness of each group after using the example / comparative example cream - average skin dermis thickness of each group before use) / average skin elasticity of each group before use * 100% Skin dermis density increase rate (%) = (average skin dermis density of each group after using the example / comparative example cream - average skin dermis density of each group before use) / average skin elasticity of each group before use * 100%

[0098] Table 7 Anti-wrinkle and anti-aging effects of the creams prepared in Examples 1 to 7 and Comparative Examples 1 to 4

[0099]

[0100] Figure 1 A, B, C, D, E, and F are Dermlab Combo skin tomographic images before using the sample, and after 28 days of using the cream in Example 3, Example 4, Example 5, Example 6, and Example 7, respectively. Different colors represent the echo intensity of different density substances in the skin. White / slightly yellow represents the epidermis, dark / green represents the low-density area, yellow / bright represents the high-density area, and the dark area represents fat, water, and subcutaneous tissue structure. In other words, the wider the green area, the thicker the dermis; the more and denser the green and yellow areas are, the higher the collagen content. Therefore, from Figure 1 It can be seen from the Dermlab Combo skin tomographic image that after 28 days of using the cream containing the three active ingredients of hexapeptide-11, algae extract and yeast / rice fermentation product filtrate in Examples 3 to 7, the dermal density and thickness of the skin increased significantly, indicating that the combination of hexapeptide-11, algae extract and yeast / rice fermentation product filtrate in a reasonable proportion can effectively increase the thickness and density of the dermis and rebuild the dermal structure.

[0101] Furthermore, from the statistical data in Table 7, it can be seen that in Examples 1 to 7 of the present invention, the higher the content of the active ingredients hexapeptide-11, golden microalgae, and yeast / rice fermentation product filtrate in the cream, the better the effect of improving skin elasticity, reducing wrinkles, and improving the thickness and density of the dermis. In addition, Comparative Examples 1 to 4 show that when only one or two active ingredients are added to the cream, the anti-aging effect (such as wrinkle removal and increasing dermal density and thickness) is not very obvious. Only when the three active ingredients of hexapeptide-11, algae extract, and yeast / rice fermentation product filtrate are added at the same time can the best anti-aging effect be exerted. This is because the yeast / rice fermentation product filtrate is rich in a variety of natural skin-nourishing ingredients (such as fruit acids, amino acids, and nucleic acids, etc.) and has excellent skin affinity and permeability, which can promote the absorption of subsequent active ingredients. It can also help rebuild the barrier function of the skin's dermal matrix and replenish the skin's own filaggrin or underlying fibers; Hexapeptide-11 can promote the expression of genes related to the skin's extracellular matrix, promote the synthesis of collagen and elastin, increase skin elasticity and firmness, and improve static wrinkles; and algae extract can further promote skin renewal and rebuild the skin's dermal structure. Therefore, the three active ingredients act together on the dermis, which can synergistically increase and repair the dermal structure (such as the skin's extracellular matrix), increase dermal density, and improve skin firmness and elasticity.

[0102] The above description of the embodiments is intended to facilitate understanding and use of the invention by those skilled in the art. It will be apparent that those skilled in the art can readily make various modifications to these embodiments and apply the general principles described herein to other embodiments without requiring inventive effort. Therefore, the present invention is not limited to the above-described embodiments. Improvements and modifications made by those skilled in the art based on the disclosure of the present invention, without departing from the scope of the present invention, should be within the scope of protection of the present invention.

Claims

1. A composition for increasing dermal thickness, characterized in that: The composition comprises the following active ingredients by weight: 0.001-2.0 parts of hexapeptide-11, 0.05-5.0 parts of algae extract, and 5-12 parts of yeast / rice fermentation product filtrate; in the composition for increasing dermal thickness, the mass ratio of hexapeptide-11, algae extract, and yeast / rice fermentation product is (1.2-2): (2-5): (8.3-11.3); The algae extract is golden microalgae; The method for preparing the yeast / rice fermentation product filtrate is as follows: (1) adding water to rice powder, heating, stirring, and obtaining 20-40 wt% rice milk, adding β-glucanase to the rice milk, wherein the amount of the enzyme added is 0.001%-0.5% of the mass of the rice milk and the enzyme activity is 150-200 U / g, and performing enzymolysis to obtain a rice milk enzymolyzate; (2) inoculating yeast into rice milk enzymatic hydrolysate, and fermenting under the following conditions: fermentation temperature 20-40° C., fermentation time 24-48 hours, pH control between 6-6.5 during the fermentation process, and obtaining rice fermentation liquid after fermentation; (3) The fermented rice fermentation liquid is concentrated by reverse osmosis technology to efficiently enrich the active substances, and then sterilized by heating and cooled and filtered to obtain the concentrated rice fermentation liquid, i.e., the yeast / rice fermentation product filtrate.

2. An anti-aging cosmetic, characterized in that: A composition for increasing dermal thickness according to claim 1.

3. The anti-aging cosmetic according to claim 2, characterized in that: The mass percentage of the composition for increasing dermal thickness in the anti-aging cosmetic is 0.1-20%.

4. The anti-aging cosmetic according to claim 2, characterized in that The anti-aging cosmetic further comprises at least one of a chelating agent, a moisturizing agent, a skin conditioner, an emollient, an emulsifier, a thickener, and a preservative.

5. The anti-aging cosmetic according to claim 4, characterized in that The chelating agent is disodium EDTA; the moisturizing agent contains at least one of glycerin, sodium hyaluronate, trehalose, honey, inositol, panthenol and betaine; the skin conditioning agent contains at least one of ceramide, pantothenic acid and magnesium gluconate; the emollient contains at least one of squalene, beeswax, jojoba seed oil, castor oil, avocado oil and avocado butter; the emulsifier contains at least one of lecithin, lanolin, lanolin alcohol, sorbitan stearate and sorbitan olivate; the thickener contains at least one of xanthan gum, carbomer, cetyl alcohol, sodium polyacrylate and tapioca starch; and the preservative is phenoxyethanol.

6. The anti-aging cosmetic according to claim 4, characterized in that The anti-aging cosmetic contains the following ingredients in percentage by mass: Chelating agent 0.01-0.02%; Moisturizer 0.5-10%; Skin conditioning agent 0.01-0.05%; Emollient 1-2%; Emulsifier 0.02-10%; Thickener 0.1-3.0%; Preservatives 0.1-1%; 0.1-20% of a composition for increasing dermal thickness; Deionized water balance.

7. A method for preparing the anti-aging cosmetic according to claim 6, characterized in that: The steps include: (1) Add a chelating agent, a moisturizing agent, a thickener, and a skin conditioning agent to deionized water, heat, homogenize, and stir to obtain a mixed solution A; (2) Heat the emulsifier and emollient, and after they are completely dissolved, add them to the mixed solution A, and add the preservative, homogenize and stir to obtain the mixed solution B; (3) Cooling the mixed solution B, adding the composition for increasing dermis thickness, stirring, and filtering.

8. Use of the composition for increasing dermal thickness according to claim 1 in the preparation of cosmetics.

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