An anti-aging and anti-wrinkle composition, a cosmetic and a preparation method thereof

Abstract

The present invention belongs to the technical field of daily-use cosmetics, and discloses an anti-aging and anti-wrinkle composition, a cosmetic and a preparation method thereof. The anti-aging and anti-wrinkle composition of the present invention comprises dipeptide diaminobutyroyl benzylamide diacetate, lysate of bifida ferment lysate and algal extract. The composition of the present invention can simultaneously improve skin aging links such as wrinkle generation, photoaging damage and extracellular matrix collapse, and achieve excellent anti-wrinkle and anti-aging effects. The cosmetic prepared from the anti-aging and anti-wrinkle composition of the present invention has excellent anti-aging performance, can effectively reduce wrinkles and improve skin elasticity; at the same time, it can also help to promote skin renewal and reconstruct the dermal structure of the skin, and solve the problem of skin aging from the root cause.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily-use cosmetics, and in particular relates to an anti-aging and anti-wrinkle composition, a cosmetic and a preparation method thereof. Background Art

[0002] As people age, the aging mechanism in the human body is activated, and aging phenomena begin to appear. The main manifestation of human aging is the aging of human skin. Skin aging is usually caused by the loss of collagen in the dermis, the activation of the oxidative stress pathway, and the influence of the external environment (such as ultraviolet radiation and air pollution, etc.). Skin aging can cause problems such as wrinkles on the face of people, decreased skin elasticity, and age spots, which affect beauty. For example, in the group standard "Evaluation Standard for Human Skin Aging" issued by the China Anti-Aging Promotion Association, the specific clinical manifestations of skin aging are elaborated. Among them, the top three manifestations of skin aging are wrinkles, elasticity, relaxation, and sagging. Therefore, cosmetics with anti-wrinkle and anti-aging effects have a huge market prospect.

[0003] Most of the existing anti-aging products only carry out anti-aging from a single approach and do not solve the problem of skin aging from the root (such as restoring cell vitality, reconstructing the skin dermal structure, etc.). Therefore, the anti-wrinkle and anti-aging effects are poor and cannot meet the anti-aging needs of consumers. In addition, although a large number of existing patents pile up various active ingredients, there is a lack of a scientific synergistic anti-aging logic among various active ingredients, and the anti-aging effect is limited. For example, in the existing patents CN114831915A and CN113952276A, a scheme of jointly using dipeptide diamino butyryl benzylamide diacetate, lysate of saccharomyces cerevisiae fermentation product and various active ingredients is disclosed. Its formula is complex and the cost is high, but the anti-aging effect is relatively average. Therefore, there is an urgent need for an active ingredient combination or product with more concise ingredients and excellent anti-aging effects (multi-path anti-aging and anti-wrinkle). Summary of the Invention

[0004] Aiming at the current situation of poor anti-wrinkle and anti-aging effects of anti-aging products in the existing technology, the purpose of the present invention is to provide an anti-aging and anti-wrinkle composition, a cosmetic and a preparation method thereof.

[0005] The technical solution adopted by the present invention is as follows:

[0006] The present invention first provides an anti-aging and anti-wrinkle composition, comprising dipeptide diamino butyryl benzylamide diacetate, lysate of saccharomyces cerevisiae fermentation product and algal extract.

[0007] Dipeptide diamino butyryl benzylamide diacetate is a synthetic peptide substance that mimics snake venom serum, which can block the nerve conduction of acetylcholine, achieve the effect of relaxing muscles, reducing wrinkles and making the skin smooth.

[0008] The lysate of Saccharomyces bisporus fermented product is rich in various active ingredients such as amino acids, lipids, polysaccharides, adenosine, vitamins and trace minerals. It can moisturize and nourish the skin, help the skin resist the damage caused by the external environment and stress, improve the skin immunosuppression caused by ultraviolet rays, promote the repair of DNA damage, reduce the damage of ultraviolet rays to the skin, and keep it active.

[0009] The algal extract contains sulfur-rich polysaccharides, which can be used as sulfur donors to promote the production of glutathione. Therefore, it has excellent antioxidant properties, can strengthen the skin's own antioxidant system, reduce oxidative damage, inhibit the degradation of matrix metalloproteinases, and thus improve wrinkles and brighten the skin.

[0010] In some alternative embodiments of the present invention, the algal extract is Nannochloropsis oculata. After Nannochloropsis oculata is cultured with an algal extract (such as Platymonas sp. S extract) and a gold salt, the alga converts "mineral gold" into active "bio-gold" (abbreviation "active gold"), enriching active ingredients such as polysaccharides, oligosaccharides, proteins and amino acids in the algal cells. Compared with ordinary algal extracts, the algal extract containing active gold has more excellent performance in promoting skin renewal, antioxidant and reconstructing the dermal structure. Nannochloropsis oculata has a very unique ability to biologically accumulate gold and gold compounds. Using this characteristic, "mineral gold" can be converted into "active gold" that can be gently repaired, thereby reducing the irritation and threat to the skin. Under certain and very specific environmental conditions, Nannochloropsis oculata can biologically accumulate gold in its cytoplasm, and these golds have been absorbed and provided in the matrix.

[0011] As a further preference of the present invention, the anti-aging and anti-wrinkle composition comprises the following components in parts by weight: 0.0001-0.2 part of dipeptide diamino butyryl benzylamide diacetate, 0.1-3 parts of the lysate of Saccharomyces bisporus fermented product and 0.05-5 parts of the algal extract.

[0012] As a further preference of the present invention, the anti-aging and anti-wrinkle composition comprises the following components in parts by weight: 0.005-0.2 part of dipeptide diamino butyryl benzylamide diacetate, 0.5-3 parts of the lysate of Saccharomyces bisporus fermented product and 0.08-2.3 parts of the algal extract.

[0013] As a further preference of the present invention, in the anti-aging and anti-wrinkle composition, the mass ratio of dipeptide diamino butyryl benzylamide diacetate, the lysate of Saccharomyces bisporus fermented product and the algal extract is (1-2):(5-16):(5-23).

[0014] The present invention further provides an anti-aging and anti-wrinkle cosmetic, which comprises the above-mentioned anti-aging and anti-wrinkle composition. The cosmetic includes makeup and skin care products such as creams, lotions, essences, masks, eye creams, etc.

[0015] Preferably, the mass percentage of the anti-aging and anti-wrinkle composition in the anti-aging and anti-wrinkle cosmetic is 0.5-10%, and without limitation, the mass percentage of the anti-aging and anti-wrinkle composition in the cosmetic includes but is not limited to 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.

[0016] As a further preference of the present invention, the anti-aging and anti-wrinkle cosmetic further comprises one or a combination of several of a chelating agent, a humectant, a skin conditioner, an emollient, an emulsifier, a thickener or water.

[0017] In some alternative embodiments of the present invention, the chelating agent is disodium EDTA; the humectant comprises at least one of glycerol, hyaluronic acid, trehalose, honey, inositol, urea, panthenol and betaine; the skin conditioner comprises at least one of ceramide and p-hydroxyacetophenone; the emollient comprises at least one of squalene, jojoba seed oil, castor oil, shea butter, beeswax and shea fruit butter; the emulsifier comprises at least one of lecithin, lanolin and lanolin alcohol; the thickener comprises at least one of xanthan gum and carbomer.

[0018] As a further preference of the present invention, the anti-aging and anti-wrinkle cosmetic contains the following components in mass percentages:

[0019] Chelating agent 0.01-0.03%;

[0020] Humectant 0.4-1%;

[0021] Skin conditioner 0.1-0.2%;

[0022] Emollient 0.3-0.5%;

[0023] Emulsifier 0.1-10%;

[0024] Thickener 0.05-5.0%;

[0025] Anti-aging and anti-wrinkle composition 0.5-10%;

[0026] Deionized water balance.

[0027] In some alternative embodiments of the present invention, the chelating agent is disodium EDTA; the humectant comprises at least one of glycerol, hyaluronic acid, trehalose, honey, inositol, urea, panthenol and betaine; the skin conditioner comprises at least one of ceramide and p-hydroxyacetophenone; the emollient comprises at least one of squalene, jojoba seed oil, castor oil, shea butter, beeswax and shea fruit butter; the emulsifier comprises at least one of lecithin, lanolin and lanolin alcohol; the thickener comprises at least one of xanthan gum and carbomer.

[0028] In some alternative embodiments of the present invention, the chelating agent is disodium EDTA; the humectant is a mixture of glycerin, sodium hyaluronate, panthenol, and betaine; the skin conditioner is isononyl isononanoate; the emollient is a mixture of jojoba seed oil, beeswax, and shea butter; the emulsifier is lecithin; and the thickener is carbomer.

[0029] The present invention provides a method for preparing an anti-aging cosmetic containing the anti-aging and anti-wrinkle composition, chelating agent, humectant, thickener, skin conditioner, emulsifier, and emollient, comprising the following steps:

[0030] (1) Add the chelating agent, humectant, thickener, and skin conditioner to deionized water, heat, homogenize, and stir to obtain a mixed solution A;

[0031] (2) Heat the emulsifier and emollient, and after they are completely dissolved, pump them into the mixed solution A, homogenize, and stir to obtain an oil-phase mixed solution B;

[0032] (3) Cool the mixed solution B, add the anti-aging and anti-wrinkle composition, stir, and filter and discharge.

[0033] The present invention further provides a method for preparing an anti-aging cosmetic containing the anti-aging and anti-wrinkle composition, chelating agent, humectant, thickener, skin conditioner, emulsifier, and emollient, comprising the following steps:

[0034] (1) Add the chelating agent, humectant, thickener, and skin conditioner to deionized water, heat to 80 - 90 °C, homogenize, and stir for 3 - 5 min to obtain an aqueous-phase mixed solution A;

[0035] (2) Heat the emulsifier and emollient to 80 - 90 °C, and after they are completely dissolved, pump them into the aqueous-phase mixed solution A, homogenize, and stir for 3 - 5 min to obtain an oil-phase mixed solution B;

[0036] (3) Cool the mixed solution B to 40 - 50 °C, add the anti-aging and anti-wrinkle composition, stir for 5 - 15 min, and filter and discharge.

[0037] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0038] (1) The anti-aging and anti-wrinkle composition of the present invention contains three active ingredients: dipeptide diamino butyryl benzylamide diacetate, lysate of bifida ferment, and algal extract. Among them, dipeptide diamino butyryl benzylamide diacetate can block the nerve conduction of acetylcholine, achieve the effect of relaxing muscles, and thus improve the dynamic wrinkles around the eyes; the lysate of bifida ferment can improve the skin immunosuppression caused by ultraviolet photoaging, promote the repair of DNA damage, reduce the damage of photoaging to the skin, and keep skin cells vital; at the same time, combined with the algal extract with excellent antioxidant, skin renewal promotion, and epidermal-dermal structure reconstruction performance, it can further effectively reduce skin aging and wrinkle phenomena. The three components cooperate with each other for skin aging links such as wrinkles, photoaging, and extracellular matrix collapse, and can achieve excellent anti-wrinkle and anti-aging effects.

[0039] (2) For the anti-aging and anti-wrinkle composition of the present invention, dipeptide diamino butyryl benzylamide diacetate is preferably used as the anti-wrinkle ingredient. Through the preferred combination and efficacy testing of the anti-aging cosmetics, the most preferred composition ratio is selected to maximize the anti-wrinkle effect; at the same time, combined with the lysate of bifida ferment and algal extract, it helps to promote skin renewal and reconstruct the dermal structure of the skin, and fundamentally solves the problem of skin aging. Brief Description of the Drawings

[0040] Figure 1 : Composition of active ingredients of the anti-aging and anti-wrinkle composition.

[0041] Figure 2 : Influence of cream treatment in different examples and comparative examples on the dermal layer thickness of the 3D full-thickness skin model.

[0042] Figure 3 : Influence of cream treatment in different examples and comparative examples on the collagen content of the 3D full-thickness skin model.

[0043] Figure 4 : Influence of cream treatment in different examples and comparative examples on the elastin content of the 3D full-thickness skin model.

[0044] Figure 5 : Influence of cream treatment in different examples and comparative examples on the fibrillin content of the 3D full-thickness skin model. Detailed Description of the Invention

[0045] The following are specific examples. Unless otherwise specified, the raw materials used in the examples are all commercially available products.

[0046] The algal extract is the golden microalgae purchased from Weidan Biotechnology Co., Ltd. (obtained by culturing Platymonas S extract and gold salt).

[0047] The present invention will be described in detail below with reference to the drawings and specific examples.

[0048] Examples 1 - 7 are the anti-aging facial creams of the present invention, and their formulations are shown in Table 1.

[0049] Table 1 Formulation Table of Examples 1 - 7 (mass percentage %)

[0050]

[0051]

[0052] The preparation methods of the anti-aging facial creams described in Examples 1 - 7 include the following steps:

[0053] (1) Add the chelating agent, humectant, thickener and skin conditioner to deionized water, heat to 90 °C, homogenize and stir for 5 min to obtain the aqueous phase mixture A;

[0054] (2) Heat the emulsifier and emollient to 90 °C, and after they are completely dissolved, add them to the aqueous phase mixture A, homogenize and stir for 5 min to obtain the oil phase mixture B;

[0055] (3) Cool the mixture B to 50 °C, add the anti-aging and anti-wrinkle composition, stir for 15 min, filter and discharge to obtain the anti-aging facial cream.

[0056] Comparative Examples 1 - 6

[0057] Comparative Examples 1 - 6 are anti-aging facial creams, and their formulations are shown in Table 2. Their preparation methods are the same as those of the examples.

[0058] Table 2 Formulation Table of Comparative Examples 1 - 6 (mass percentage %)

[0059]

[0060]

[0061] Performance Test

[0062] (1) Product Safety Assessment

[0063] Test method: Use the human skin patch test to evaluate the product safety.

[0064] Test substance: The facial creams prepared in Examples 1 - 7.

[0065] Test subjects: 30 subjects were selected for each group for testing (15 males and 15 females), meeting the volunteer selection criteria for subjects.

[0066] Patch test method: Select a qualified patch tester. Using the closed patch test method, apply about 0.02 - 0.025 g of the facial creams prepared in Examples 5 - 10 into the patch tester, and then apply a special tape externally on the flexor side of the forearm of the subject. After 24 hours, remove the test substance. 30 minutes after removing the patch tester with the test substance, wait for the indentation to disappear and then observe the skin reaction. Record the result according to the skin reaction grading standard in "Cosmetics Safety Technical Regulations 2015". Record the reaction results in Table 3 according to the following adverse reaction grading standard.

[0067] Remark: Skin reaction grading standard

[0068] Grade 0: Negative reaction;

[0069] Grade 1: Suspected reaction; Only faint erythema;

[0070] Grade 2: Weak positive reaction (erythema reaction); Erythema, infiltration, edema, and may have papules;

[0071] Grade 3: Strong positive reaction (herpes reaction); Erythema, infiltration, edema, with papules, and the reaction extends beyond the test area;

[0072] Grade 4: Extremely strong positive reaction (confluent herpes reaction); Obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0073] Table 3 Results of the patch test of the facial creams on human skin in Examples 1 - 7

[0074]

[0075] As can be seen from Table 3, after the facial creams prepared in Examples 1 - 7 were applied to the flexor side of the arm, all 30 subjects in each group showed a negative reaction, with a grade of 0. According to the judgment standard of the patch test on human skin in "Cosmetics Safety Technical Regulations 2015", the facial creams prepared in Examples 1 - 7 have no adverse reactions to human skin and have passed the safety assessment.

[0076] (2) Stability assessment

[0077] Test samples: Examples 1 - 7

[0078] Test method: For the heat resistance test, place the facial cream at 45 ± 2 °C for three months and observe the appearance, color, and odor of the facial cream; for the cold resistance test, place the facial cream at -18 ± 2 °C for three months and observe the appearance, color, and odor of the facial cream; for the heat and cold cycling test, store the facial cream at 45 °C, room temperature, and -18 °C for 24 hours each, and cycle 3 times, and then observe the appearance, color, and odor of the facial cream. The test results are shown in Table 4.

[0079] Table 4 Stability test results

[0080]

[0081]

[0082] As can be seen from Table 4, the anti-aging cream with anti-wrinkle and anti-aging effects of the present invention has excellent stability, and can ensure that the basic physical and chemical properties remain unchanged under high temperature, low temperature, and high-low temperature cycling conditions.

[0083] (3) In vitro cell experiments

[0084] The gene expression of normal human epidermal keratinocytes and normal human dermal fibroblasts was evaluated by microfluidic RT-qPCR. The specific operations are as follows: Normal human epidermal keratinocytes and normal human dermal fibroblasts were inoculated into 96-well plates at an appropriate cell density and incubated in a 37 °C cell culture incubator until the cell confluence rate was approximately 80%; subsequently, a serum-free cell culture medium (containing 0%, 1.5 wt% golden microalgae, 1.5 wt% Tetraselmis suecica extract) was added and incubated for 24 hours. Finally, the differences in the expression of 90 genes in normal human epidermal keratinocytes and normal human dermal fibroblasts were analyzed by microfluidic RT-qPCR. There were 6 replicates in each group of experiments and repeated 3 times. The changes in gene expression were expressed as a percentage of the control group (pure cell culture medium), and the average value of each group was taken. The gene expression change rate (%) of golden microalgae and Tetraselmis suecica extract = (B - A) / A * 100%, where A is the epidermal growth factor expression level after treatment with a serum-free cell culture medium (containing 0% golden microalgae or Tetraselmis suecica extract), and B is the epidermal growth factor expression level after treatment with a serum-free cell culture medium (containing 1.5 wt% golden microalgae or Tetraselmis suecica extract). The experimental results are shown in Table 5.

[0085] Table 5 Transcriptomic gene analysis test results of different microalgae extracts (change rate compared to the control group)

[0086]

[0087]

[0088] As can be seen from Table 5, compared with the Tetraselmis suecica extract without gold salt treatment at the same concentration, golden microalgae has a stronger effect in reconstructing the epidermal and dermal layers, such as upregulating filaggrin, epidermal growth factor, collagen 1, elastin, and collagen 17, etc., and downregulating matrix metalloproteinase-1 and matrix metalloproteinase-3 genes.

[0089] (4) Anti-aging effect of in vitro 3D full-thickness skin model

[0090] The 3D full-thickness skin model FulKutis has an epidermis layer and a dermis layer, which is relatively similar to human skin. Therefore, it is often used to simulate the anti-aging effect of the skin after applying cosmetics. In this embodiment, the 3D full-thickness skin model FulKutis is used as the detection model, and UVA irradiation (30 J / cm 2 ) is used to simulate photoaging damage. To evaluate the anti-aging and wrinkle-reducing effects of the creams in Example 9 and Comparative Examples 1-7, the UVA-treated 3D full-thickness skin model FulKutis is placed in a medium at 37 °C and 5% CO2 for 5 days to stabilize it, and then different cream samples are applied to its surface once in the morning and once in the evening (continuously for 7 days). The treated samples are fixed in formalin, embedded in paraffin, and then cut into thin slices with a thickness of 4 μm. To observe the change in the thickness of the skin dermis layer, the skin sections are stained with hematoxylin-eosin staining solution (H&E) kit according to the instructions. To observe the changes in collagen I, elastin, and fibrillin in the skin, the skin sections are incubated and stained with rabbit monoclonal anti-type I collagen (Abcam, Cambridge, MA, USA), mouse monoclonal anti-elastin (Abcam), and mouse anti-fibrillin 1 (Abcam) antibodies respectively. Then, photographs are taken and observed using an optical microscope, and Image J software is used for image analysis and quantitative statistics of the biomarker staining intensity. The normal 3D skin (i.e., the control normal skin group) is used as the negative control group, and the UVA-irradiated skin group (i.e., the UVA group) is used as the photoaging model group. The statistical data are shown in Table 6 and Figures 2 to 5 as follows.

[0091] Table 6 Quantitative statistical table of anti-aging effect data of 3D full-thickness skin model

[0092]

[0093] As shown in Table 6 and Figures 2 to 5 as follows, after UVA radiation treatment, the thickness of the dermis layer in the 3D full-thickness skin model decreased from 250 μm to 110 μm, and the contents of collagen, elastin, and fibrillin also decreased significantly, indicating that the photoaging model was successfully established. After applying the cream in Example 5 (containing 0.1% dipeptide diamino butyryl benzylamide diacetate, 1.4% lysate of Saccharomyces cerevisiae fermentation products, and 1.5% algal extract), the thickness of the dermis layer increased from 110 μm to 230 μm, and the contents of collagen, elastin, and fibrillin also increased significantly, approaching the content composition in normal skin. After treatment with the creams in Comparative Examples 1-6, the thickness of the dermis layer, collagen, elastin, and fibrillin in the 3D full-thickness skin model improved compared with the UVA radiation group, but the improvement rate was not high, indicating that when the cream contains three active ingredients of dipeptide diamino butyryl benzylamide diacetate, lysate of Saccharomyces cerevisiae fermentation products, and algal extract at a certain ratio, the best anti-aging effect can be achieved.

[0094] It can be seen that the three active ingredients, dipeptide diamino butyryl benzylamide diacetate, lysate of bifida ferment, and algal extract, have a significant synergistic effect. The effect during the synergistic action is far better than that of a single component or two of them.

[0095] (5) Wrinkle reduction and anti-aging efficacy test

[0096] Three hundred and ninety healthy female volunteers (aged 45 - 55) were selected and divided into 13 groups with 30 people in each group. They used the creams in Examples 1 - 7 and Comparative Examples 1 - 6 respectively. The participants used the samples twice a day, morning and evening, for 56 consecutive days. During the test period, the participants were not allowed to use other products. The test results were evaluated after 56 days of using the cream samples. Before the start of the test, the skin wrinkles and skin elasticity of the participants were measured; after 7 days, 14 days, 28 days, and 56 days of using the cream products, the skin wrinkles and skin elasticity of the participants were measured again. Among them, the skin wrinkle condition was measured using a skin image analysis system (Skin Visiometer SV600); the skin elasticity R2 was measured using a dual MPA580 skin elasticity detector. The measurement and statistical results are shown in Tables 7 and 8.

[0097] Facial wrinkle improvement rate (%) = (Test wrinkle parameter value after using different creams - Test wrinkle parameter value before using different creams) / Test wrinkle parameter value before using different creams. The wrinkle improvement rate in Table 8 is the positive value after taking the change rate calculated by the formula.

[0098] Facial elasticity improvement rate (%) = (Test elasticity value after using different creams - Test elasticity value before using different creams) / Test elasticity value before using different creams. The elasticity improvement rate in Table 7 is the positive value after taking the change rate calculated by the formula.

[0099] Table 7 Facial wrinkle improvement rates of the creams in Examples 1 - 5 and Comparative Examples 1 - 6 after using for different times

[0100]

[0101] Table 8 Elasticity improvement rates of the creams in Examples 1 - 7 and Comparative Examples 1 - 6 after using for different times

[0102]

[0103]

[0104] As can be seen from Table 7 and Table 8, the higher the contents of dipeptide diamino butyroyl benzylamide diacetate, lysate of bifida ferment, and algal extract in the cream, the better the effects of wrinkle removal and skin elasticity restoration. After using the cream in Examples 1 to 7 for seven days, excellent anti-wrinkle and anti-aging effects can be achieved. The longer the using time, the better the anti-wrinkle and anti-aging effects. This is because dipeptide diamino butyroyl benzylamide diacetate can block the nerve conduction of acetylcholine to achieve the effect of relaxing muscles, thereby improving dynamic eye wrinkles; the lysate of bifida ferment can improve skin immunosuppression caused by ultraviolet photoaging, promote the repair of DNA damage, reduce the damage of photoaging to the skin, and keep skin cells active; meanwhile, combined with the algal extract with excellent antioxidant properties, promoting skin renewal, and reconstructing the epidermal-dermal structure performance, it can further effectively reduce skin aging and wrinkles. The three components cooperate with each other for skin aging links such as wrinkles, photoaging, and extracellular matrix collapse, and can achieve excellent anti-wrinkle and anti-aging effects.

[0105] The data in Table 7 and Table 8 also show that the anti-wrinkle and anti-aging performance of the cream in Example 5 is significantly better than that of Comparative Examples 1 to 6, indicating that the active ingredients of the anti-aging composition of the present invention cooperate with each other for skin anti-aging links to maximize the anti-wrinkle and anti-aging effects. In summary, the cream containing the anti-aging and anti-wrinkle composition of dipeptide diamino butyroyl benzylamide diacetate, lysate of bifida ferment, and algal extract of the present invention has excellent anti-wrinkle and anti-aging effects.

[0106] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art without departing from the scope of the present invention should be within the protection scope of the present invention.

Claims

1. An anti-aging and anti-wrinkle composition, characterized in that, It includes dipeptide diamino butyryl benzylamide diacetate, lysate of bifida ferment lysate, and algal extract; and the mass ratio of dipeptide diamino butyryl benzylamide diacetate, lysate of bifida ferment lysate, and algal extract is (1 - 2):(5 - 16):(5 - 23); The algal extract is the golden microalgae purchased from Weidan Biotechnology Co., Ltd.

2. An anti-aging cosmetic, characterized in that, It contains the anti-aging and anti-wrinkle composition as described in Claim 1.

3. The anti-aging cosmetic according to claim 2, characterized in that, The anti-aging cosmetic further contains one or several of chelating agents, humectants, skin conditioners, emollients, emulsifiers, thickeners, or water.

4. The cosmetic according to claim 2 or 3, characterized in that, The mass percentage of the anti-aging and anti-wrinkle composition in the cosmetic is 0.5% - 10%.

5. The anti-aging cosmetic according to claim 3, characterized in that, The chelating agent is disodium EDTA; the humectant contains at least one of glycerol, hyaluronic acid, trehalose, honey, inositol, urea, panthenol, and betaine; the skin conditioner contains at least one of ceramide and p-hydroxyacetophenone; the emollient contains at least one of squalene, jojoba seed oil, castor oil, shea butter, beeswax, and shea fruit butter; the emulsifier contains at least one of lecithin, lanolin, and lanolin alcohol; the thickener contains at least one of xanthan gum and carbomer.

6. The anti-aging cosmetic according to claim 5, wherein The anti-aging cosmetic contains the following components in mass percentage: Chelating agent 0.01 - 0.1%; Humectant 0.4 - 10%; Skin conditioner 0.1 - 10%; Emollient 0.3 - 10%; Emulsifier 0.1 - 10%; Thickener 0.05 - 5.0%; Anti-aging and anti-wrinkle composition 0.5 - 10%; Deionized water balance.

7. A method for preparing the anti-aging cosmetic according to any one of claims 2 to 6, characterized in that, It includes the following steps: (1) Add the chelating agent, humectant, thickener, and skin conditioner to deionized water, heat, homogenize, and stir to obtain a mixed solution A; (2) Heat the emulsifier and emollient, and after they are completely dissolved, pump them into the mixed solution A, homogenize, and stir to obtain an oil-phase mixed solution B; (3) Cool down the mixed solution B, add the anti-aging and anti-wrinkle composition, stir, and filter and discharge.

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