Method, device, medium and equipment for judging insufficient dose of hemolytic agent

Abstract

The application discloses a kind of hemolytic agent underdosing judgment method, device, medium and equipment, by obtaining the particle distribution condition of signal intensity in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of signal intensity of each class in the particle distribution condition of

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hemolytic agent, and in particular to a method, device, medium and equipment for judging insufficient dosage of hemolytic agent. BACKGROUND

[0002] The blood cell analyzer can count various blood cells in blood. For example, in one scenario, after 5LDS hemolytic agent is mixed with fresh blood sample, red blood cells are lysed, and white blood cells are stained, then 5LHS hemolytic agent is added, and the 5LHS alkaline hemolytic agent denudes the white blood cells except basophilic granulocytes (BASO), so that BASO cells and the remaining cells are obviously distinguished in volume, thereby distinguishing BASO.

[0003] The detection principle is as shown in Figure 1 Under the wrapping of the sheath liquid, the cells to be tested are arranged in a single row and flow into the flow chamber at a uniform speed. Under the irradiation of the laser beam, three different angle scattering lights are generated. The size of the scattering light generated by the irradiation of the laser beam is related to the size of the cell, the refractive index of the complexity of the cell membrane and the internal organs of the cell. The scattering light signal is finally converted into an electric pulse signal. According to the collected electric pulse data, the scatter plot distribution of the white blood cells under the three-dimensional signal can be obtained. Finally, the classification result of the white blood cells is obtained according to the white blood cell scatter plot.

[0004] When the blood cell analyzer measures the sample, for example, if the required 5LHS hemolytic reagent in the above scenario is insufficient, the white blood cell scatter plot will be abnormal, thereby causing abnormal classification of white blood cells, and finally the reported cell count result is inaccurate. The traditional method for detecting the residual amount of hemolytic agent is to detect the liquid level of the hemolytic agent by a sensor to judge the residual amount of the reagent. However, this method needs hardware support, and its principle is relatively complex, the device is large in size, and because there are many components, once a component fails, the entire detection device cannot work normally. In addition, the sensor detection is greatly affected by interference factors. For example, the most common bubble interference, when there is a bubble, it may misjudge the insufficient hemolytic agent. Therefore, a simple, fast and effective method is needed to remind the insufficient amount of hemolytic agent. SUMMARY

[0005] Therefore, it is necessary to provide a method, device, medium and equipment for judging insufficient dosage of hemolytic agent to solve the problem of inaccurate white blood cell detection caused by insufficient dosage of hemolytic agent.

[0006] A method for judging insufficient dosage of hemolytic agent, the method comprising:

[0007] obtaining a set of pulse signals of a blood cell sample to be tested at a preset scattering angle, identifying signal intensity of each pulse signal in the set of pulse signals, and counting particle distribution of the signal intensity; wherein the particle distribution is used to indicate particle number of blood cell particles with different signal intensity, and the blood cell sample to be tested is obtained after the blood cell sample is treated by a current hemolytic agent;

[0008] calculating particle number change rate of each type of signal intensity in the particle distribution to obtain a change rate condition;

[0009] searching for a falling zero point in the change rate condition, and determining a local maximum value of particle number in the particle distribution based on the searched falling zero point;

[0010] if the number of local maximum values of particle number is greater than 1, it is determined that the current hemolytic agent is seriously insufficient in dosage;

[0011] if the number of local maximum values of particle number is equal to 1, intensity mean and intensity standard deviation of signal intensity under different scattering angles are calculated, and a correlation coefficient is calculated based on the intensity mean and the intensity standard deviation;

[0012] if the correlation coefficient is less than a preset threshold, it is determined that the current hemolytic agent is slightly insufficient in dosage; if the correlation coefficient is greater than or equal to the preset threshold, it is determined that the current hemolytic agent is sufficient in dosage.

[0013] In one embodiment, the calculation formula of the particle number change rate is:

[0014] S i = La i+1 -La i , (i∈1, 2, 3, …N-1)

[0015] In the above formula, S i indicates the particle number change rate of the i-th type of signal intensity, La i+1 indicates the particle number of blood cell particles of the i+1-th type of signal intensity, La i indicates the particle number of blood cell particles of the i-th type of signal intensity, and N indicates the total number of types of signal intensity.

[0016] In one embodiment, the searching for a falling zero point in the change rate condition, and determining a local maximum value of particle number in the particle distribution based on the searched falling zero point, comprises:

[0017] searching for all falling zero points in the change rate condition, and obtaining particle number corresponding to each falling zero point in the particle distribution as the local maximum value of particle number.

[0018] In one of the embodiments, the intensity mean value is calculated according to the following formula:

[0019]

[0020] In the above formula, indicates the intensity mean value corresponding to the characteristic value k, indicates the signal intensity of the a-th cell in the blood cell sample to be measured at the preset scattering angle corresponding to the characteristic value k, and A indicates the total number of cells;

[0021] The intensity standard deviation is calculated according to the following formula:

[0022]

[0023] In the above formula, indicates the intensity standard deviation corresponding to the characteristic value k.

[0024] In one of the embodiments, the correlation coefficient is calculated according to the following formula:

[0025]

[0026] In the above formula, is the correlation coefficient, and the characteristic values 1 and 2 are any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light.

[0027] In one of the embodiments, after the particle distribution of the signal intensity is counted, the method further comprises:

[0028] filtering the particle distribution; wherein the filtering formula is:

[0029]

[0030] In the above formula, F(Ful i ) is the particle distribution after filtering, Ful i is the particle distribution before filtering, i indicates the i-th signal intensity, N indicates the total number of signal intensities, u indicates the mean value, and σ indicates the standard deviation.

[0031] A device for judging insufficient dosage of a hemolytic agent, the device comprising:

[0032] a particle distribution determining module, configured to acquire a pulse signal set of a blood cell sample to be measured at a preset scattering angle, identify the signal intensity of each pulse signal in the pulse signal set, and count the particle distribution of the signal intensity; wherein the particle distribution is used to indicate the particle number of blood cell particles with different signal intensities, and the blood cell sample to be measured is a blood cell sample obtained after being treated by a current hemolytic agent;

[0033] The extreme point determination module is configured to calculate the particle number change rate of each type of signal intensity in the particle distribution condition to obtain a change rate condition, search for a falling zero point in the change rate condition, and determine a local particle number maximum value in the particle distribution condition based on the searched falling zero point.

[0034] The judgment module is configured to determine that the current hemolytic agent dosage is severely insufficient if the number of local particle number maximum values is greater than 1, calculate the intensity mean and intensity standard deviation of signal intensity under different scattering angles, and calculate a correlation coefficient based on the intensity mean and the intensity standard deviation if the number of local particle number maximum values is equal to 1, determine that the current hemolytic agent dosage is slightly insufficient if the correlation coefficient is less than a preset threshold, and determine that the current hemolytic agent dosage is sufficient if the correlation coefficient is greater than or equal to the preset threshold.

[0035] A computer readable storage medium stores a computer program, and the computer program is executed by a processor to make the processor execute the steps of the method for judging the hemolytic agent dosage insufficiency.

[0036] A device for judging hemolytic agent dosage insufficiency includes a memory and a processor, and the memory stores a computer program, and the computer program is executed by the processor to make the processor execute the steps of the method for judging the hemolytic agent dosage insufficiency.

[0037] The present application provides a method, device, medium and equipment for judging hemolytic agent dosage insufficiency, which acquires a pulse signal set of a blood cell sample to be tested under a preset scattering angle, counts a particle distribution condition of signal intensity, calculates the particle number change rate of each type of signal intensity in the particle distribution condition, searches for a falling zero point in the change rate condition, and determines a local particle number maximum value in the particle distribution condition based on the searched falling zero point, to judge whether the current hemolytic agent dosage is insufficient. If the number of local particle number maximum values is greater than 1, it is determined that the current hemolytic agent dosage is severely insufficient, and if the number of local particle number maximum values is equal to 1, the correlation coefficient is calculated to judge whether the hemolytic agent dosage is slightly insufficient or sufficient. It can be seen that the present application can monitor the hemolytic agent dosage based on the local particle number maximum value and the correlation coefficient, can reduce the cost required for instrument detection, makes the instrument structure more simple, and is not affected by bubble interference factors, and is accurate enough. BRIEF DESCRIPTION OF DRAWINGS

[0038] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the accompanying drawings needed to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the accompanying drawings in the following description only represent some embodiments of the present application, and all other drawings obtained by those of ordinary skill in the art without creative effort based on these drawings belong to the protection scope of the present application.

[0039] Wherein:

[0040] Figure 1 A schematic diagram of the principle of white blood cell monitoring;

[0041] Figure 2 A schematic diagram of the flow of the method for judging insufficient dosage of hemolytic agent;

[0042] Figure 3 A schematic diagram of generating three different angle scattered light;

[0043] Figure 4 A scatter plot of signal intensity of white blood cells and particle distribution in the case of serious insufficient hemolytic agent;

[0044] Figure 5 A schematic diagram of the extreme point in the particle distribution; Figure 4

[0045] Figure 6 A correlation coefficient of the blood cell samples (a), (b) and (c) to be tested;

[0046] Figure 7 A schematic diagram of the structure of the judging device for insufficient dosage of hemolytic agent;

[0047] Figure 8 A structural block diagram of the judging device for insufficient dosage of hemolytic agent. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments only represent some embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort belong to the protection scope of the present application.

[0049] ​The terms "first", "second", and the like in the description and in the claims of the present application and above drawings are used for distinguishing between similar objects, not necessarily described in a particular order. Also, the terms "comprise", "comprising", and the like are intended to encompass non-exclusive inclusions. For example, processes, methods, articles, or apparatuses that comprise a list of steps or elements are not limited to the listed steps or elements, but can also comprise additional steps or elements not expressly listed, or can also comprise steps or elements inherent in such processes, methods, articles, or apparatuses.

[0050] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment can be included in at least one embodiment of the application. The appearances of the phrase that an embodiment in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of one another. As will be apparent to those of ordinary skill in the art, embodiments described herein can be combined with one another.

[0051] As Figure 2 shown, Figure 2 is a flowchart of a method for judging insufficient dosage of a hemolytic agent in an embodiment. The method for judging insufficient dosage of a hemolytic agent in the embodiment provides the following steps:

[0052] S201, obtaining a set of pulse signals of a blood cell sample to be tested at a preset scattering angle, identifying the signal intensity of each pulse signal in the set of pulse signals, and counting the particle distribution of the signal intensity.

[0053] The particle distribution is used to indicate the number of blood cell particles of different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent is processed.

[0054] For example, in one scenario, the blood cell sample to be tested is first processed with sufficient 5LDS hemolytic agent, the red blood cells are lysed, and the white blood cells are dyed, then the current 5LHS hemolytic agent is added, the 5LHS basic hemolytic agent will naked the white blood cells except BASO, so that BASO cells and the remaining cells are obviously different in volume, so that white blood cells and red blood cell fragments can be obtained. Under the wrapping of the sheath liquid, the cells are arranged in a single row and flow into the flow chamber at a constant speed, under the irradiation of the laser beam, the reference Figure 3The scattered light of three different angles is generated. The generated scattered light includes low-angle scattered light, middle-angle scattered light and high-angle scattered light. The low-angle scattered light is the scattered light in the forward low-angle region, the middle-angle scattered light is the scattered light in the forward middle-angle region, and the high-angle scattered light is the scattered light in the lateral high-angle region. The low-angle scattered light can reflect the size of the cell, the middle-angle scattered light can reflect the internal fine structure and particulate matter of the cell, and the high-angle forward scattered light can also reflect the internal fine structure and particulate matter of the cell. The diaphragm of the receiving part is used to determine whether there is scattered light, the first receiver receives the middle-angle scattered light emitted from the flow chamber and converts it into a middle-angle pulse signal, thereby constituting a pulse signal set corresponding to the middle angle; the second receiver receives the high-angle scattered light emitted from the flow chamber and converts it into a high-angle pulse signal, thereby constituting a pulse signal set corresponding to the high angle; and the third receiver receives the low-angle scattered light emitted from the flow chamber and converts it into a low-angle pulse signal, thereby constituting a pulse signal set corresponding to the low angle.

[0055] Further, the signal intensity of each pulse signal in the pulse signal set is identified by using an existing pulse identification algorithm, such as a threshold detection algorithm or an energy threshold algorithm. a , (a∈1, 2, 3, …A) represents the signal intensity of the pulse signal of the a-th white blood cell in the sample, where A represents the total number of pulse signals.

[0056] Further, based on the signal intensities, a signal intensity scatter plot of the white blood cells under three-dimensional signals can be obtained. For example, as shown in FIG. 4(a), in the plot, the Y-axis-LS represents the signal intensity of the low-angle scattered light, and the X-axis-MS represents the signal intensity of the middle-angle scattered light. Of course, the scatter plot can also be constructed based on the scattered light of other angles. Figure 4

[0057] Further, the particle distribution of the signal intensity is counted, which is used to indicate the particle number of the blood cell particles of different signal intensities, where La i , (i=1, 2, 3, …N) indicates the particle number of the i-th signal intensity, and N is the total number of signal intensities. For example, as shown in FIG. 4(b), the scatter plot in FIG. 4(a) is mapped in the LS direction, and the particle distribution as shown in FIG. 4(b) can be obtained. Figure 4 Figure 4

[0058] In one specific embodiment, the particle distribution is also subjected to filtering processing; and the formula of the filtering processing is:

[0059]

[0060] In the above formula, F (Ful i ​​​) is the particle distribution after filtering, F is the filter, u indicates the mean, σ indicates the standard deviation, i indicates the i-th signal intensity, and N indicates the total number of signal intensities. i is the particle distribution before filtering, i indicates the i-th signal intensity, N indicates the total number of signal intensities, u indicates the mean, and σ indicates the standard deviation.

[0061] After the above filtering, noise in the particle distribution can be effectively removed, and the overall distribution is smoother.

[0062] S202, the rate of change of the number of particles of each signal intensity in the particle distribution is calculated to obtain a rate of change.

[0063] Based on the rate of change, the trend of the number of particles at each signal intensity in the particle distribution can be understood.

[0064] In one embodiment, the formula for calculating the rate of change of the number of particles of the i-th signal intensity is:

[0065] S i = La i+1 -La i , (i∈1, 2, 3, …N-1)

[0066] In the above formula, S i indicates the rate of change of the number of particles of the i-th signal intensity, La i+1 indicates the number of particles of blood cells of the i+1-th signal intensity, La i indicates the number of particles of blood cells of the i-th signal intensity, and N indicates the total number of signal intensities.

[0067] S203, search for a falling zero point in the rate of change, and determine a local maximum of the number of particles in the particle distribution based on the searched falling zero point.

[0068] In the rate of change, the local range to the left of the falling zero point is greater than 0, indicating that the number of particles of the corresponding signal intensity in the particle distribution is always increasing; the local range to the right of the falling zero point is less than 0, indicating that the number of particles of the corresponding signal intensity in the particle distribution is always decreasing, and the falling zero point can be found in the rate of change based on the above conditions. Optionally, the falling zero point is defined as, for a rate of change of the number of particles of the i-th signal intensity S i , if the following conditions are met, it is determined as a falling zero point:

[0069]

[0070] Then, based on the searched falling zero points, the local maximum of the number of particles in the particle distribution can be determined.

[0071] In one embodiment, the particle number local maximum is determined by searching all falling zero points in the rate of change and obtaining the particle number corresponding to each falling zero point in the particle distribution as the particle number local maximum. For example, in Figure 5 , the particle numbers corresponding to A, B and C are determined as the particle number local maximum.

[0072] S204, the number of particle number local maximum is compared with 1. If the number of particle number local maximum is greater than 1, S205 is executed to determine that the current hemolytic agent dosage is severely insufficient. If the number of particle number local maximum is equal to 1, S206 is executed.

[0073] For example, as shown in Figure 5 , the number of particle number local maximum is 3, which is greater than 1, so it is determined that the current hemolytic agent dosage is severely insufficient.

[0074] This is because, if the required hemolytic reagent is severely insufficient, the white blood cells other than the cells to be detected will not be completely nucleated, Figure 4 (a) more abnormal points (points in the box range) other than the cells to be detected will appear in the scatter plot, and after mapping, Figure 4 (b) multiple particle number local maximums (extra particle number local maximums in the oval area) will appear in the particle distribution.

[0075] On the contrary, if the number of particle number local maximum is equal to 1, it means that the current hemolytic agent dosage is not severely insufficient, and further accurate determination can be made through subsequent steps.

[0076] S206, the intensity mean and intensity standard deviation of the signal intensity at different scattering angles are calculated, and the correlation coefficient is calculated based on the intensity mean and the intensity standard deviation.

[0077] The calculation formula of the intensity mean is:

[0078]

[0079] In the above formula, indicates the intensity mean corresponding to the characteristic value k, indicates the signal intensity of the a-th cell in the blood cell sample to be tested at the preset scattering angle corresponding to the characteristic value k, and A indicates the total number of cells.

[0080] The calculation formula of the intensity standard deviation is:

[0081]

[0082] In the above formula, indicates the intensity standard deviation corresponding to the characteristic value k.

[0083] wherein the correlation coefficient is calculated by the formula:

[0084]

[0085] In the above formula, is the correlation coefficient, and the eigenvalues 1, 2 are any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light. The size of the correlation coefficient reflects the correlation of the signal intensity of the particles at different scattering angles, and the greater the value, the stronger the correlation.

[0086] For example, referring to Figure 6 , Figure 6 is the correlation coefficient of the blood cell sample (a), (b), (c) to be tested, wherein the eigenvalues 1, 2 selected are the signal intensity of low-angle scattered light and the signal intensity of medium-angle scattered light. The correlation coefficient of the blood cell sample (a) to be tested is 92.234. The correlation coefficient of the blood cell sample (b) to be tested is 38.114. The correlation coefficient of the blood cell sample (c) to be tested is 10.454.

[0087] S207, determine whether the correlation coefficient is less than a preset threshold value. If the correlation coefficient is less than the preset threshold value, execute S208 to determine that the current hemolytic agent dosage is slightly insufficient. If the correlation coefficient is greater than or equal to the preset threshold value, execute S209 to determine that the current hemolytic agent dosage is sufficient.

[0088] Optionally, the threshold value is set to 60, so it can be determined that Figure 6 the blood cell sample (a) to be tested uses a sufficient amount of hemolytic agent; Figure 6 the blood cell samples (b), (c) to be tested use a slightly insufficient amount of hemolytic agent; and Figure 6 the hemolytic agent dosage of (c) is less than Figure 6 the hemolytic agent dosage of (b). This is because under the premise that the hemolytic reagent is sufficient, the leukocyte bare except for the cells to be tested will be completely karyotic, and there will be no small amount of abnormal points except for the cells to be tested in the scatter plot, and only one obvious cluster will appear. Under this condition, the correlation between the eigenvalues is strong, and the correlation coefficient is also large. With the gradual decrease of the hemolytic reagent, the leukocyte bare except for the cells to be tested will not be completely karyotic, and there will be a small amount of abnormal points except for the cells to be tested in the scatter plot, and the cluster will gradually disperse, as shown in Figure 6 (b), (c), at which time the correlation between the eigenvalues is weakened, and the correlation sparsity is also reduced.

[0089] The method for judging insufficient dose of hemolytic agent, by obtaining the pulse signal set of the blood cell sample to be measured at the preset scattering angle, and counting the particle distribution of the signal intensity, then calculating the particle number change rate of each type of signal intensity in the particle distribution, searching for the falling zero point in the change rate condition, and determining the local maximum of the particle number in the particle distribution based on the searched falling zero point, to judge whether the current dose of hemolytic agent is insufficient. If the number of local maximum of the particle number is greater than 1, it is determined that the current dose of hemolytic agent is severely insufficient; if the number of local maximum of the particle number is equal to 1, the correlation coefficient is calculated to judge whether the dose of hemolytic agent is slightly insufficient or sufficient. It can be seen that the present application can monitor the dose of hemolytic agent based on the local maximum of the particle number and the correlation coefficient, can reduce the cost required for instrument detection, make the instrument structure more simple, and is not affected by interference factors such as bubbles, and is accurate enough.

[0090] In one embodiment, as shown in Figure 7 a device for judging insufficient dose of hemolytic agent is proposed, which comprises:

[0091] The particle distribution condition determining module 701 is used for obtaining the pulse signal set of the blood cell sample to be measured at the preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution of the signal intensity; wherein the particle distribution is used to indicate the particle number of blood cell particles with different signal intensities, and the blood cell sample to be measured is the blood cell sample obtained after being treated by the current hemolytic agent;

[0092] The extreme point determining module 702 is used for calculating the particle number change rate of each type of signal intensity in the particle distribution, to obtain the change rate condition; searching for the falling zero point in the change rate condition, and determining the local maximum of the particle number in the particle distribution based on the searched falling zero point;

[0093] The judging module 703 is used for determining that the current dose of hemolytic agent is severely insufficient if the number of local maximum of the particle number is greater than 1; calculating the intensity mean and intensity standard deviation of the signal intensity at different scattering angles if the number of local maximum of the particle number is equal to 1, and calculating the correlation coefficient based on the intensity mean and intensity standard deviation; determining that the current dose of hemolytic agent is slightly insufficient if the correlation coefficient is less than a preset threshold; and determining that the current dose of hemolytic agent is sufficient if the correlation coefficient is greater than or equal to the preset threshold.

[0094] In one embodiment, the calculation formula of the particle number change rate is:

[0095] S i =La i+1 -La i , (i∈1,2,3,…N-1)

[0096] In the above formula, S i a rate of change of the number of particles indicating the signal intensity of the i-th type, La i+1 a number of particles of blood cell particles indicating the signal intensity of the i+1-th type, La i a number of particles of blood cell particles indicating the signal intensity of the i-th type, N indicates the total number of types of signal intensity.

[0097] In one embodiment, the extreme point determination module 702 is specifically configured to:

[0098] search for all falling zero points in the rate of change, and obtain the number of particles corresponding to each falling zero point in the particle distribution as a local maximum of the number of particles.

[0099] In one embodiment, the calculation formula of the intensity mean value is:

[0100]

[0101] In the above formula, k indicates the intensity mean value corresponding to the eigenvalue k, indicates the signal intensity of the a-th cell in the blood cell sample to be measured at the preset scattering angle corresponding to the eigenvalue k, A indicates the total number of cells;

[0102] The calculation formula of the intensity standard deviation is:

[0103]

[0104] In the above formula, k indicates the intensity standard deviation corresponding to the eigenvalue k.

[0105] In one embodiment, the calculation formula of the correlation coefficient is:

[0106]

[0107] In the above formula, k is the correlation coefficient, and the eigenvalues 1 and 2 are any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light.

[0108] In one embodiment, the insufficient hemolytic agent dosage determination device is further configured to:

[0109] perform filtering processing on the particle distribution; wherein the formula of the filtering processing is:

[0110]

[0111] In the above formula, F(Ful i ) is the particle distribution after filtering processing, Fuli The particle distribution before filtering is represented by i, where i indicates the intensity of the i-th type of signal, N indicates the total number of signal types, u indicates the mean, and σ indicates the standard deviation.

[0112] Figure 8 An internal structural diagram of a device for determining insufficient hemolytic agent dosage is shown in one embodiment. Figure 8 As shown, the device for determining insufficient hemolytic agent dosage includes a processor, a memory, and a network interface connected via a system bus. The memory includes a non-volatile storage medium and internal memory. The non-volatile storage medium stores an operating system and may also store a computer program. When executed by the processor, this computer program enables the processor to implement a method for determining insufficient hemolytic agent dosage. The internal memory may also store a computer program, which, when executed by the processor, enables the processor to implement the method for determining insufficient hemolytic agent dosage. Those skilled in the art will understand that... Figure 8 The structure shown is merely a block diagram of a portion of the structure related to the present application and does not constitute a limitation on the hemolytic agent dosage determination device applied thereto. The specific hemolytic agent dosage determination device may include more or fewer components than those shown in the figure, or combine certain components, or have different component arrangements.

[0113] A computer-readable storage medium storing a computer program, which, when executed by a processor, performs the following steps: acquiring a set of pulse signals from a blood cell sample to be tested at a preset scattering angle; identifying the signal intensity of each pulse signal in the set of pulse signals; and statistically analyzing the particle distribution of the signal intensities; wherein the particle distribution indicates the number of blood cell particles with different signal intensities; the blood cell sample to be tested is a blood cell sample obtained after treatment with a current hemolysin; calculating the rate of change of the number of particles for each type of signal intensity in the particle distribution to obtain the rate of change; and searching... The system identifies the zero-crossing point in the rate of change and determines the local maximum of the number of particles in the particle distribution based on the found zero-crossing point. If the number of local maxima of the number of particles is greater than 1, the current dose of the hemolytic agent is determined to be severely insufficient. If the number of local maxima of the number of particles is equal to 1, the system calculates the mean intensity and standard deviation of the signal intensity at different scattering angles and calculates the correlation coefficient based on the mean intensity and standard deviation. If the correlation coefficient is less than a preset threshold, the current dose of the hemolytic agent is determined to be slightly insufficient. If the correlation coefficient is greater than or equal to the preset threshold, the current dose of the hemolytic agent is determined to be sufficient.

[0114] A lytic agent dosage deficiency judging device, comprising a memory, a processor and a computer program stored in the memory and executable on the processor, the processor implements the following steps when executing the computer program: obtaining a pulse signal set of a blood cell sample to be tested at a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution of the signal intensity; wherein the particle distribution indicates the particle number of blood cell particles of different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current lytic agent treatment; calculating the particle number change rate of each signal intensity in the particle distribution to obtain a change rate condition; searching for a falling zero point in the change rate condition, and determining a local maximum particle number in the particle distribution based on the searched falling zero point; if the number of local maximum particle numbers is greater than 1, it is determined that the current lytic agent dosage is severely insufficient; if the number of local maximum particle numbers is equal to 1, the intensity mean and intensity standard deviation of the signal intensity at different scattering angles are calculated, and the correlation coefficient is calculated based on the intensity mean and the intensity standard deviation; if the correlation coefficient is less than a preset threshold, it is determined that the current lytic agent dosage is slightly insufficient; and if the correlation coefficient is greater than or equal to the preset threshold, it is determined that the current lytic agent dosage is sufficient.

[0115] It should be noted that the lytic agent dosage deficiency judging method, device, equipment and computer readable storage medium belong to one overall inventive concept, and the contents in the lytic agent dosage deficiency judging method, device, equipment and computer readable storage medium embodiments can be mutually applicable.

[0116] Those skilled in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be completed by instructing the relevant hardware through a computer program, and the program can be stored in a non-volatile computer readable storage medium. When the program is executed, it can include the processes of the above-mentioned embodiment methods. Any reference to memory, storage, database or other medium used in the embodiments provided in the present application can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. As an illustration but not limitation, RAM is available in various forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDR SDRAM), enhanced SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.

[0117] Any combination of the technical features of the above embodiments can be made. In order to make the description simple, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combination of the technical features does not exist, it should be considered as the scope of the present application.

[0118] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent of the present application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A method for determining a hemolytic agent dose deficiency, characterized by, The method comprises: acquiring a pulse signal set of a blood cell sample to be tested at a preset scattering angle, identifying signal intensity of each pulse signal in the pulse signal set, and counting particle distribution of the signal intensity; wherein the blood cell sample to be tested is obtained after the current hemolytic agent is processed, and the particle distribution is used to indicate the particle number of blood cell particles with different signal intensities; calculating the particle number change rate of each type of signal intensity in the particle distribution to obtain a change rate condition; searching for a falling zero point in the change rate condition, and determining a local maximum particle number in the particle distribution based on the searched falling zero point; if the number of local maximum particle numbers is greater than 1, it is determined that the current hemolytic agent is seriously insufficient in dosage; if the number of local maximum particle numbers is equal to 1, the intensity mean and intensity standard deviation of the signal intensity at different scattering angles are calculated, and the correlation coefficient is calculated based on the intensity mean and the intensity standard deviation; if the correlation coefficient is less than a preset threshold, it is determined that the current hemolytic agent is slightly insufficient in dosage; if the correlation coefficient is greater than or equal to the preset threshold, it is determined that the current hemolytic agent is sufficient in dosage.

2. The method of claim 1, wherein, The calculation formula of the particle number change rate is: S i = La i+1 -La i , (i e 1,2,3,... N-1) In the above formula, S i a rate of change of the number of particles indicative of the intensity of the i-th signal, La i+1 a number of particles indicative of the blood cell particles of the intensity of the i+1-th signal, La i a number of particles indicative of the blood cell particles of the intensity of the i-th signal, N indicates the total number of classes of signal intensity.

3. The method of claim 1, wherein, The searching for the falling zero point in the change rate condition and the determining of the local maximum particle number in the particle distribution based on the searched falling zero point comprise: searching for all falling zero points in the change rate condition, and acquiring the particle number corresponding to each falling zero point in the particle distribution as the local maximum particle number.

4. The method of claim 1, wherein, The calculation formula of the intensity mean is: In the above formula, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity average value corresponding to the characteristic value k, an intensity The calculation formula of the intensity standard deviation is: In the above formula, indicates the intensity standard deviation corresponding to the characteristic value k.

5. The method of claim 4, wherein, The calculation formula of the correlation coefficient is: In the above formula, are the correlation coefficients, and the eigenvalues 1, 2 are any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light.

6. The method of claim 1, wherein, After the counting of the particle distribution of the signal intensity, the method further comprises: filtering the particle distribution; wherein the filtering formula is: In the above formula, F (Ful i ) is the particle distribution after filtering processing, Ful i is the particle distribution before filtering processing, i indicates the i-th signal intensity, N indicates the total number of signal intensities, u indicates the mean, and σ indicates the standard deviation.

7. A device for determining a dose deficiency of a hemolytic agent, characterized in that The device comprises: a particle distribution condition determining module, configured to acquire a pulse signal set of a blood cell sample to be tested at a preset scattering angle, identify signal intensity of each pulse signal in the pulse signal set, and count particle distribution of the signal intensity; wherein the blood cell sample to be tested is obtained after the current hemolytic agent is processed, and the particle distribution is used to indicate the particle number of blood cell particles with different signal intensities; an extreme point determining module, configured to calculate the particle number change rate of each type of signal intensity in the particle distribution to obtain a change rate condition, search for a falling zero point in the change rate condition, and determine a local maximum particle number in the particle distribution based on the searched falling zero point. The judgment module is configured to determine that the current dose of the hemolytic agent is seriously insufficient if the number of the particle number local maxima is greater than 1; calculate the intensity mean and the intensity standard deviation of the signal intensity under different scattering angles, and calculate a correlation coefficient based on the intensity mean and the intensity standard deviation if the number of the particle number local maxima is equal to 1; determine that the current dose of the hemolytic agent is slightly insufficient if the correlation coefficient is less than a preset threshold; and determine that the current dose of the hemolytic agent is sufficient if the correlation coefficient is greater than or equal to the preset threshold.

8. A computer-readable storage medium, characterized in that, A computer program is stored, and the computer program, when executed by a processor, causes the processor to perform the steps of the method according to any one of claims 1 to 6.

9. A device for determining insufficient dosage of hemolytic agent, characterized in that, A computer program is stored, and the computer program, when executed by a processor, causes the processor to perform the steps of the method according to any one of claims 1 to 6.

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