An inducible hair follicle regeneration and hair growth promoting traditional Chinese medicine shampoo and a preparation method thereof

Abstract

The application discloses a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth and a preparation method thereof, and relates to the technical field of daily chemical products. The shampoo comprises the following components in parts by weight: Bacillus amyloliquefaciens fermentation extract 10-20 parts, Amoora roxburghii Benth extract 25-40 parts, tryptophan 1-4 parts, Myristica fragrans Houtt extract 20-35 parts, Platycladus orientalis (L.) Franco extract 20-30 parts, seaweed extract 18-26 parts, surfactant 6-15 parts, foaming agent 1-3 parts, thickening agent 0.5-1.5 parts, lubricant 0.1-0.8 parts, humectant 0.5-2.5 parts and water 800-1000 parts. The effective components of the shampoo can rapidly penetrate into the keratin layer barrier of the scalp and be absorbed by the scalp, promote the proliferation of hair follicle stem cells, prolong the growth period of the hair follicles, delay the entry of the hair follicles into the involution period, prevent the atrophy of the hair follicles and realize rapid hair growth.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of daily chemical products, in particular to a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth and a preparation method thereof. BACKGROUND

[0002] Due to factors such as life tension, increased stress, and changes in eating habits, seborrheic alopecia and alopecia areata have become the main types of hair loss today, and the patient population has expanded from older men to young people and female groups. Hair loss affects the psychological and social relationship health of individuals, and has been identified as the second largest social disease. Therefore, research in the direction of promoting hair growth has received great attention from the world of medicine and cosmetics. Shampoo is the hair care product used most frequently in people's daily life, and the development of hair growth promoting shampoo has received much attention.

[0003] Most of the hair growth promoting shampoos on the market add traditional Chinese medicine extracts to achieve hair growth and hair loss prevention effects through effective traditional Chinese medicine ingredients. Patent application number 202010839720.2 discloses a hair loss preventing and hair growth promoting shampoo and a preparation method thereof, which mainly uses salvia miltiorrhiza extract, angelica sinensis extract, safflower extract, radix astragali extract, radix polygoni multiflori extract, and oil tea seed polypeptide to promote hair growth. Patent application number 202110240572.7 discloses a formula of a hair growth and hair blackening shampoo and a preparation method thereof, which mainly uses palm leaf, eclipta prostrata, cuscuta chinensis, hibiscus leaf and hibiscus flower, biota leaf, campsis flower, pomegranate flower, and flaxseed to promote hair growth. These methods have achieved certain effects, but the mechanisms are relatively single. There are few shampoos that comprehensively study the directions of inducing hair follicle regeneration, prolonging the hair follicle growth period, and preventing hair follicle atrophy to achieve hair growth effects. In addition, most of these effective ingredients are macromolecular substances, which are difficult to penetrate into the scalp keratin layer barrier and be absorbed by the scalp. Moreover, the effective medicinal ingredients are difficult to play their maximum role due to the short residence time of shampoo on the scalp.

[0004] In view of the above, the present application aims to provide a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth and a preparation method thereof to solve the above problems. SUMMARY

[0005] In view of the above, the present application aims to provide a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth and a preparation method thereof to solve the above problems.

[0006] The application discloses a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth, which comprises the following components in parts by weight: Bacillus amyloliquefaciens fermentation extract 10-20 parts, Amorphophallus konjac root extract 25-40 parts, tryptophan 1-4 parts, Myristica fragrans extract 20-35 parts, Platycladus orientalis extract 20-30 parts, seaweed extract 18-26 parts, surfactant 6-15 parts, foaming agent 1-3 parts, thickening agent 0.5-1.5 parts, lubricant 0.1-0.8 parts, humectant 0.5-2.5 parts and water 800-1000 parts.

[0007] Further, the preparation method of the Bacillus amyloliquefaciens fermentation extract is as follows: after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is firstly performed, the upper liquid is uniformly mixed with an ethanol solution, continuous stirring is performed for 1-2 hours, centrifugal separation is performed, the upper solution is concentrated under reduced pressure to half of the original volume, then, ethyl acetate extraction is performed, the solvent is recovered by heating, and extract A is obtained; after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is firstly performed, under ice bath conditions, ammonium sulfate powder is added to the upper liquid while stirring, so that the mass concentration of ammonium ions is 25-30 g / L, then, standing is performed at 4-5 DEG C until the precipitate is separated out, the precipitate is separated out, an equal amount of water and 0.2-0.5% of the weight of the precipitate of protease are added to the precipitate and treated for 20-30 minutes, and enzyme inactivation is performed, and extract B is obtained; the extract A and the extract B are mixed in a weight ratio of 1:3-6, and the Bacillus amyloliquefaciens fermentation extract is obtained.

[0008] Further, the preparation method of the Amorphophallus konjac root extract is as follows: after crushing of Amorphophallus konjac root, 10-20 volume times of ethanol solution is added for extraction for 10-20 hours, extraction is repeated for 2-3 times, the extraction liquid is combined, and concentration is performed to half of the original volume, and the Amorphophallus konjac root extract is obtained.

[0009] Further, the preparation method of the Myristica fragrans extract is as follows: Myristica fragrans dry mature seeds are crushed into powder, 10-15 times of the weight of the powder of water and 0.1-0.5% of cellulase are added to the powder, treatment is performed for 2-4 hours, saturated lime water solution is then added for ultrasonic extraction for 2-3 times, the extraction liquid is combined, pH is adjusted to neutral, macroporous resin is then added for adsorption, and ethanol solution is used for elution, the elution liquid is placed to crystallize, and crushing is performed, and the Myristica fragrans extract is obtained.

[0010] Further, the Platycladus orientalis extract is composed of Platycladus orientalis volatile oil components and Platycladus orientalis water-soluble components in a volume ratio of 1:50-100.

[0011] Further, the seaweed extract preparation method is: first, the seaweed is crushed and then soaked in hydrochloric acid with a mass concentration of 10-20% for 3-4 days, after removing the acid solution, water washing is performed until neutral, then sodium hydroxide solution with a mass concentration of 10-15% is added and heated to boiling for 1-2 hours, after removing the alkali solution, water washing is performed until neutral, then sodium hydroxide solution with a mass concentration of 40-55% is added and microwave heating is performed for 10-20 minutes, after removing the alkali solution, water washing is performed until neutral, drying, crushing, and then the seaweed extract is obtained.

[0012] A preparation method of a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth, comprising the following steps:

[0013] (1) Take raw materials by weight fraction, mix the bacillus amyliqueferment extract, the scaphium macropodium extract, the tryptophan, the myristica extract and the biota orientalis extract uniformly, obtain a mixed material, mix the mixed material with lanolin alcohol with a weight fraction of 1-5% of the mixed material, and then stir at a speed of 200-250 r / min for 30-50 min to obtain a first material;

[0014] (2) Add the seaweed extract into water with a temperature of 60-70 DEG C and 10-20 times of the seaweed extract by weight, and then stir at a speed of 200-250 r / min for 20-30 min to obtain a second material;

[0015] (3) Mix the first material and the second material, and then add them into a high shear reaction kettle, and then perform high shear at a speed of 2000-3500 r / min at 30-50 DEG C for 1-2 h, and then perform suction filtration to obtain a third material;

[0016] (4) Put the third material, the surfactant, the foam booster, the thickening agent, the lubricant, the humectant and water into a homogenizer, and then homogenize for 20-30 min to obtain the shampoo.

[0017] Further, the surfactant is any one or a combination of two of lauryl glucoside, ammonium laureth sulfate, potassium cocoyl hydrolyzed oat protein and polyethylene glycol stearate.

[0018] Further, the foam booster is alkylolamide or cocamidopropyl betaine, and the thickening agent is one of xanthan gum, guar gum or hydroxyethyl cellulose.

[0019] Further, the lubricant is silicone oil, and the humectant is propylene glycol or sodium hyaluronate.

[0020] It should be noted that the scaphium macropodium in the present application is a herbaceous plant of the genus scaphium in the cucurbitaceae family, the myristica is a plant of the genus myristica in the zingiberaceae family, and the biota orientalis is a plant of the genus biota in the cupressaceae family.

[0021] Compared with the prior art, the present application has the following beneficial effects:

[0022] 1.The shampoo can clean hair follicles, prevent hair follicle atrophy, and induce hair follicle regeneration and promote hair growth. The Bacillus amyloliquefaciens fermentation liquor, the Amorphophallus konjac seed extract, and the tryptophan can promote the proliferation of hair follicle stem cells, and have antibacterial and anti-inflammatory effects. The Myristica extract can accelerate the hair follicles from the resting phase to the growth phase, prolong the time of the hair follicles in the growth phase, and promote hair growth. The Platycladus orientalis leaf extract can clean the hair follicles and prevent hair follicle atrophy. The Bacillus amyloliquefaciens fermentation liquor, the Amorphophallus konjac seed extract, the tryptophan, the Myristica extract, and the Platycladus orientalis leaf extract can synergistically increase the number of hair follicles, ensure the health of the hair follicles, and promote the growth of the hair.

[0023] 2.The preparation method can wrap the effective medicinal ingredients such as the Bacillus amyloliquefaciens fermentation extract, the Amorphophallus konjac seed extract, the tryptophan, the Myristica extract, and the Platycladus orientalis leaf extract in the seaweed extract to form the hydrogel particles with a nanoscale particle size. The seaweed extract has high similarity with the skin keratin lipid layer, can carry the effective components to quickly penetrate into the skin barrier, is easily absorbed by the scalp, and fully plays a role and has a more lasting effect. DETAILED DESCRIPTION

[0024] The specific embodiments of the present application are described in detail below, but it should be understood that the protection scope of the present application is not limited by the specific embodiments.

[0025] Example 1

[0026] A traditional Chinese medicine shampoo for inducing hair follicle regeneration and promoting hair growth, the shampoo comprises the following components in parts by weight: Bacillus amyloliquefaciens fermentation extract 10 parts, Amorphophallus konjac seed extract 25 parts, tryptophan 1 part, Myristica extract 20 parts, Platycladus orientalis leaf extract 20 parts, seaweed extract 18 parts, lauryl glucoside 6 parts, alkylolamide 1 part, xanthan gum 0.5 part, silicone oil 0.1 part, propylene glycol 0.5 part, and water 800 parts.

[0027] The preparation method of the Bacillus amyloliquefaciens fermentation extract is as follows: after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is performed at 1000 r / min, 100 mL of supernatant is mixed with 40 mL of 60% ethanol solution, stirring is performed for 1 h, centrifugal separation is performed, the supernatant is concentrated to half of the original volume under reduced pressure, and extraction is performed with ethyl acetate, then the solvent is recovered by heating, and the extract A is obtained; after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is performed at 1000 r / min, and under ice bath conditions, ammonium sulfate powder is added to the supernatant while stirring, so that the mass concentration of ammonium ions is 25 g / L, then the supernatant is placed at 4 ℃ until the precipitate is separated out, the precipitate is separated out, an equal amount of water and 0.2% of the weight of the precipitate of protease are added to the precipitate, and the mixture is treated for 20 min, the enzyme is inactivated, and the mixture is dried and crushed to obtain the extract B; the extract A and the extract B are mixed in a weight ratio of 1:3 to obtain the Bacillus amyloliquefaciens fermentation extract.

[0028] The preparation method of the Jatropha gossypiifolius extract is as follows: after the tuberous roots of Jatropha gossypiifolius are crushed, 10 times of 60% ethanol solution by volume is added for extraction for 10 h, the extraction is repeated twice, the extraction solutions are combined, and the combined solution is concentrated to half of the original volume to obtain the Jatropha gossypiifolius extract.

[0029] The preparation method of the Myristica fragrans extract is as follows: Myristica fragrans dry mature seeds are crushed into powder, 10 times of water by weight of the powder, and 0.1% of cellulase are added to the powder, and the mixture is treated for 2 h, then saturated lime water solution is added for ultrasonic extraction twice, the extraction solutions are combined, the pH of the combined solution is adjusted to neutral, then macroporous resin is added for adsorption, and the adsorbed solution is eluted with an ethanol solution, the eluate is left to crystallize, and the crystallized product is crushed to obtain the Myristica fragrans extract. The elution with the ethanol solution is as follows: 3 times of 20% ethanol solution by mass concentration is used to elute impurities, and then 5 times of 60% ethanol solution by mass concentration is used to elute.

[0030] The Cacumen biotae extract is composed of Cacumen biotae volatile oil components and Cacumen biotae water-soluble components in a volume ratio of 1:50.

[0031] The preparation method of the seaweed extract is as follows: seaweed is crushed, then soaked in 10% hydrochloric acid for 3 days, washed with water to neutral after removal of the acid solution, then boiled in 10% sodium hydroxide solution for 1 h, washed with water to neutral after removal of the alkali solution, then microwave-heated in 40% sodium hydroxide solution for 10 min, washed with water to neutral after removal of the alkali solution, dried, and crushed to obtain the seaweed extract.

[0032] The preparation method of the traditional Chinese medicine shampoo in the embodiment includes the following steps:

[0033] (1) Take the raw materials by weight fraction, mix the Bacillus amyloliquefaciens fermentation extract, the Morinda citrifolia extract, the tryptophan, the Myristica fragrans extract and the Biota orientalis extract uniformly, get the mixed material, mix the mixed material with 1% of the wool fat alcohol of the mixed material by weight, stir at the speed of 200 r / min for 30 min, get the first material;

[0034] (2) Add the seaweed extract into 10 times of 60℃ water of the seaweed extract by weight, stir at the speed of 200 r / min for 20 min, get the second material;

[0035] (3) Mix the first material and the second material, add to the high shear reaction kettle, carry out high shear at 30℃, 2000 r / min speed for 1h, suction filtration, get the third material;

[0036] (4) Put the third material and the lauryl glucoside, alkylolamide, xanthan gum, silicone oil, propylene glycol and water into the homogenizer, homogenize for 20 min, get the shampoo.

[0037] Example 2

[0038] A traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth, the shampoo comprises the following components by weight fraction: Bacillus amyloliquefaciens fermentation extract 20 parts, Morinda citrifolia extract 40 parts, tryptophan 4 parts, Myristica fragrans extract 35 parts, Biota orientalis extract 30 parts, seaweed extract 26 parts, ammonium lauryl polyoxyethylene sulfate 15 parts, cocamidopropyl betaine 3 parts, guar gum 1.5 parts, silicone oil 0.8 parts, sodium hyaluronate 2.5 parts and water 1000 parts.

[0039] The preparation method of the Bacillus amyloliquefaciens fermentation extract is as follows: after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is carried out at 1500 r / min, 100 mL of the upper layer liquid is mixed with 50 mL of 80% ethanol solution, stirred for 2h, centrifuged, the upper layer solution is concentrated under reduced pressure to half of the original volume, extracted with ethyl acetate, heated to recover the solvent, get extract A; after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is carried out at 1500 r / min, under ice bath condition, ammonium sulfate powder is added to the upper layer liquid while stirring, so that the mass concentration of ammonium ion is 30g / L, then, stand at 5℃ until the precipitate is separated out, separate the precipitate, add equal amount of water and 0.5% protease of the weight of the precipitate to the precipitate, treat for 30 min, inactivate the enzyme, dry, crush, get extract B; mix the extract A and the extract B in a weight ratio of 1:6, get the Bacillus amyloliquefaciens fermentation extract.

[0040] The preparation method of the Morinda citrifolia extract is as follows: the tuberous roots of Morinda citrifolia are crushed, then 20 times of 70% ethanol solution by volume is added for extraction for 20 h, the extraction is repeated for 3 times, the extraction solutions are combined, and the combined solution is concentrated to half of the original volume to obtain the Morinda citrifolia extract.

[0041] The preparation method of the Myristica fragrans extract is as follows: the dried mature seeds of Myristica fragrans are crushed into powder, 15 times of water by weight of the powder and 0.5% cellulase are added to the powder for treatment for 4 h, then saturated lime water solution is added for ultrasonic extraction for 3 times, the extraction solutions are combined, the pH is adjusted to neutral, then macroporous resin is added for adsorption, and the adsorbed product is eluted with ethanol solution, the eluate is left to crystallize, and the crystallized product is crushed to obtain the Myristica fragrans extract. The elution with ethanol solution is as follows: 8 times of 50% ethanol solution by weight is used to elute impurities, and then 15 times of 75% ethanol solution by weight is used to elute.

[0042] The Cacumen Platycladi extract is composed of Cacumen Platycladi volatile oil component and Cacumen Platycladi water-soluble component in a volume ratio of 1:100.

[0043] The preparation method of the seaweed extract is as follows: the seaweed is crushed, then soaked in 20% hydrochloric acid for 4 days, washed with water to neutral after removing the acid solution, then heated and boiled in 15% sodium hydroxide solution for 2 h, washed with water to neutral after removing the alkali solution, then microwave-heated in 55% sodium hydroxide solution for 20 min, washed with water to neutral after removing the alkali solution, dried, and crushed to obtain the seaweed extract.

[0044] The preparation method of the traditional Chinese medicine shampoo in the embodiment includes the following steps:

[0045] (1) The raw materials are weighed, and the Bacillus amyliqueferment extract, Morinda citrifolia extract, tryptophan, Myristica fragrans extract and Cacumen Platycladi extract are mixed uniformly to obtain a mixture, the mixture is mixed with 5% lanolin alcohol by weight of the mixture, and stirred at a speed of 250 r / min for 50 min to obtain a first material;

[0046] (2) The seaweed extract is added to 20 times of 70°C water by weight of the seaweed extract, and stirred at a speed of 250 r / min for 30 min to obtain a second material;

[0047] (3) The first material and the second material are mixed and added to a high-shear reaction kettle, and high-shear treatment is carried out at 50°C and a rotation speed of 3500 r / min for 2 h, and then the mixture is filtered to obtain a third material;

[0048] (4) The third material, ammonium lauryl ether sulphate, cocamidopropyl betaine, guar gum, silicone oil, sodium hyaluronate and water are put into a homogenizer, and homogenized for 30 min to obtain the shampoo.

[0049] Example 3

[0050] An inducible hair follicle regeneration and hair growth promoting traditional Chinese medicine shampoo, the shampoo comprises the following components by weight: Bacillus amyloliquefaciens fermentation extract 15 parts, Amorphophallus konjac tuber extract 30 parts, tryptophan 2 parts, Myristica extract 30 parts, Platycladus orientalis extract 25 parts, seaweed extract 23 parts, potassium cocoyl hydrolyzed oat protein 5 parts, polyethylene glycol stearate 8 parts, alkylolamide 2 parts, hydroxyethyl cellulose 1.0 part, silicone oil 0.4 part, propylene glycol 2.0 parts and water 900 parts.

[0051] The preparation method of the Bacillus amyloliquefaciens fermentation extract is as follows: after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is carried out at 1200 r / min, 100 mL of supernatant is mixed with 60 mL of 70% ethanol solution, stirring is carried out for 1.5 h, centrifugal separation is carried out, the supernatant is concentrated under reduced pressure to half of the original volume, and after extraction with ethyl acetate, the solvent is recovered by heating to obtain extract A; after liquid fermentation culture of Bacillus amyloliquefaciens, centrifugal separation is carried out at 1300 r / min, and under ice bath conditions, ammonium sulfate powder is added to the supernatant while stirring to make the mass concentration of ammonium ion 27 g / L, then, standing is carried out at 4.5℃ until the precipitate is separated out, the precipitate is separated out, and the same amount of water and 0.4% protease of the weight of the precipitate are added to the precipitate for 25 min of treatment, enzyme inactivation, drying, and crushing to obtain extract B; the extract A and the extract B are mixed in a weight ratio of 1:5 to obtain the Bacillus amyloliquefaciens fermentation extract.

[0052] The preparation method of the Amorphophallus konjac tuber extract is as follows: after crushing of Amorphophallus konjac tuber, 15 times of 75% ethanol solution by volume is added for 15 h of extraction, the extraction is repeated for 3 times, the extraction liquid is combined, and concentrated to half of the original volume to obtain the extract.

[0053] The preparation method of the Myristica extract is as follows: Myristica dry mature seeds are crushed into powder, 13 times of water by weight of the powder, and 0.3% cellulase are added to the powder for 3 h of treatment, then saturated lime water solution is added for 3 times of ultrasonic extraction, the extraction liquid is combined, the pH is adjusted to neutral, then macroporous resin is added for adsorption, and ethanol solution is used for elution, the eluate is placed to crystallize, and crushing is carried out to obtain the extract. The ethanol solution elution is as follows: 5 times of 40% ethanol solution by mass concentration is used for elution of impurities, and then 10 times of 65% ethanol solution by mass concentration is used for elution.

[0054] The Platycladus orientalis extract is composed of Platycladus orientalis volatile oil component and Platycladus orientalis water-soluble component in a volume ratio of 1:80.

[0055] The preparation method of the seaweed extract is as follows: seaweed is crushed and then soaked in 15% hydrochloric acid for 4 days, washed with water to neutral after removing the acid, then heated and boiled in 12% sodium hydroxide solution for 1.5 hours, washed with water to neutral after removing the alkali, then microwave heated in 50% sodium hydroxide solution for 13 minutes, washed with water to neutral after removing the alkali, dried, crushed, and then obtained.

[0056] The preparation method of the traditional Chinese medicine shampoo in the embodiment comprises the following steps:

[0057] (1) Take raw materials by weight fraction, mix the bacillus amyliqueferment extract, the morinda officinalis extract, the tryptophan, the mace extract and the biota orientalis extract uniformly, obtain a mixed material, mix the mixed material with 2% lanolin alcohol of the mixed material weight, stir at a speed of 220 r / min for 40 min, and obtain a first material;

[0058] (2) Add the seaweed extract into 15 times seaweed extract weight of 65℃ water, stir at a speed of 220 r / min for 25 min, and obtain a second material;

[0059] (3) Mix the first material and the second material, add into a high shear reaction kettle, and perform high shear at 40℃ and a rotation speed of 3000 r / min for 1.5 hours, and then perform suction filtration, and obtain a third material;

[0060] (4) Put the third material, the cocoyl hydrolyzed oat protein potassium, the polyethylene glycol stearate, the alkylol amide, the hydroxyethyl cellulose, the silicone oil, the propylene glycol and the water into a homogenizer, and homogenize for 30 min, and then obtain the shampoo.

[0061] Comparative Example 1

[0062] The present comparative example provides a traditional Chinese medicine shampoo, and the formula table is different from that of Example 1 only in that the bacillus amyloliquefaciens fermentation liquid is not contained, and other components and contents remain unchanged. The preparation method is referred to Example 1.

[0063] Comparative Example 2

[0064] The present comparative example provides a traditional Chinese medicine shampoo, and the formula table is different from that of Example 1 only in that the morinda officinalis extract is not contained, and other components and contents remain unchanged. The preparation method is referred to Example 1.

[0065] Comparative Example 3

[0066] The present comparative example provides a traditional Chinese medicine shampoo, and the formula table is different from that of Example 1 only in that the tryptophan is not contained, and other components and contents remain unchanged. The preparation method is referred to Example 1.

[0067] Comparative Example 4

[0068] The comparative example provides a traditional Chinese medicine shampoo. The formulation table is the same as that of Example 1, except that the extract of Myristica fragrans is not contained, and the other components and contents remain unchanged. The preparation method is referred to Example 1.

[0069] Comparative Example 5

[0070] The comparative example provides a traditional Chinese medicine shampoo. The formulation table is the same as that of Example 1, except that the extract of Platycladus orientalis is not contained, and the other components and contents remain unchanged. The preparation method is referred to Example 1.

[0071] Comparative Example 6

[0072] The comparative example provides a traditional Chinese medicine shampoo. The formulation table is the same as that of Example 1, except that the preparation method of Bacillus amyloliquefaciens fermentation extract is different. The comparative example is prepared by obtaining extract A, and extract B is not prepared.

[0073] Comparative Example 7

[0074] The comparative example provides a traditional Chinese medicine shampoo. The formulation table is the same as that of Example 1, except that the preparation method of Bacillus amyloliquefaciens fermentation extract is different. The comparative example is prepared by obtaining extract B, and extract A is not prepared.

[0075] Comparative Example 8

[0076] The comparative example provides a traditional Chinese medicine shampoo. The formulation table is the same as that of Example 1, but the preparation method is carried out according to the conventional method, i.e., all the effective medicinal ingredients are mixed first, and then the mixture is mixed with surfactants, foam boosters, thickening agents, lubricants, humectants, and water to obtain a homogeneous product. The extract of seaweed is not used for preparation.

[0077] Animal experiments

[0078] (1) Test method:

[0079] Forty 12-week-old test mice (provided by the Animal Experiment Center of Guangxi Medical University) were taken. After the mice were caged and fed for 10 days, the back hair was first cut short along the parallel spine of the mouse as the long axis direction, and then the experimental animal depilatory was applied to the corresponding position to remove the back hair of the mouse. The removal area was 2 cm x 2 cm (test area). Thirty-six mice with no damage in the depilation area were selected and randomly divided into 12 groups, 3 mice in each group. The shampoo was applied to the back test area of the mice with a cotton swab, washed clean after 10 minutes of rubbing, and applied at a fixed time every day for 28 consecutive days, wherein:

[0080] Groups 1 to 3 applied the shampoos prepared in Examples 1 to 3, respectively;

[0081] Group 4 to group 11 respectively apply the shampoo prepared by comparative example 1 to comparative example 8;

[0082] Group 12 applies physiological saline as a blank control group.

[0083] (2) Test results:

[0084] A. Take photos under the same light source every day, record the color change of the skin of the test area on the back of each group of mice, record the time T1 required for the color of the skin of the test area to change from pink to black (i.e. the time for the hair follicles to enter the growth phase from the resting phase), and the time T2 required for the color to change from black to gray (i.e. the duration of the hair follicles in the growth phase), and calculate the average value.

[0085] B. On the 28th day, 10 new hairs were randomly taken from the test area on the back of the mice, and the length was measured using a percentage electronic vernier caliper, and the average value was calculated.

[0086] C. At the 28th day of the experiment, the skin on the back of the mice was cut, paraffin sectioned, and stained for observation, and the average number of hair follicles was calculated.

[0087] The experimental results are shown in Table 1:

[0088] Table 1: Hair growth of mice in each group

[0089]

[0090] Conclusion: 1. The comparative data of example 1 and comparative examples show that Bacillus amyloliquefaciens fermentation liquor, Morinda citrifolia extract, tryptophan, Myristica extract and Platycladus orientalis extract work together, synergistically, and effectively increase the number of hair follicles, ensure the health of hair follicles, and promote the growth of hair. 2. The preparation method of Bacillus amyloliquefaciens fermentation extract will affect the content and type of effective components, and thus affect the proliferation of hair follicles. 3. The preparation method will affect the use effect of the shampoo, and the preparation method of the present application can improve the absorption of effective components by the scalp.

[0091] Safety tests

[0092] The shampoo prepared by example 1 to example 3 was applied to the back of 60 experimental mice for 24 hours, and compared with the unapplied parts, there was no redness or other allergic phenomena, which indicated that the shampoo of the present application was mild and non-irritating.

[0093] Detection of active ingredient action time

[0094] Twelve test volunteers were recruited and randomly divided into A, B, C, D groups, 3 people in each group. A group, B group, C group used the shampoo prepared by the method of embodiment 1, embodiment 2, embodiment 3 respectively, and D group used the shampoo prepared by the method of comparative example 8. After use, the time (average) of the active ingredients in the shampoo at 90% equivalent release rate was analyzed by near-infrared spectroscopy analyzer, as shown in table 2:

[0095] Table 2: Active ingredient action time of each group

[0096]

[0097] Table 2 shows that under the condition of equivalent release rate, the release time of the shampoo prepared by the method of the present application is longer, the active ingredient action time is more durable, and the hair growth effect is more significant, which is better than the existing preparation method.

[0098] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments and its various modifications as are suited to the particular use contemplated. It is intended that the scope of the application be defined by the claims and their equivalents.

Claims

1. A method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth, characterized in that, The shampoo comprises the following components in parts by weight: 10-20 parts of Bacillus amyloliquefaciens fermentation extract, 25-40 parts of Aralia elata extract, 1-4 parts of tryptophan, 20-35 parts of cardamom extract, 20-30 parts of Platycladus orientalis leaf extract, 18-26 parts of seaweed extract, 6-15 parts of surfactant, 1-3 parts of foaming agent, 0.5-1.5 parts of thickener, 0.1-0.8 parts of lubricant, 0.5-2.5 parts of moisturizer, and 800-1000 parts of water; The preparation method of the Bacillus amyloliquefaciens fermentation extract is as follows: After liquid fermentation culture of Bacillus amyloliquefaciens, centrifugation is performed first. The supernatant is mixed with ethanol solution and stirred continuously for 1-2 hours. After centrifugation, the supernatant is concentrated under reduced pressure to half of its original volume. Then, it is extracted with ethyl acetate, and the solvent is recovered by heating to obtain extract A. After liquid fermentation culture of Bacillus amyloliquefaciens, centrifugation is performed first. Under ice bath conditions, ammonium sulfate powder is added to the supernatant while stirring to make the ammonium ion mass concentration 25-30 g / L. Then, it is allowed to stand at 4-5℃ until precipitation occurs. The precipitate is separated, and an equal amount of water and 0.2-0.5% of the weight of the precipitate of protease are added to the precipitate for 20-30 minutes to inactivate the enzyme and obtain extract B. Extract A and extract B are mixed in a weight ratio of 1:3-6 to obtain the Bacillus amyloliquefaciens fermentation extract. The specific preparation method includes the following steps: (1) Take the raw materials according to the weight parts, mix the Bacillus amyloliquefaciens fermentation extract, large-seed snow gall extract, tryptophan, cardamom extract and arborvitae leaf extract evenly to obtain a mixture, mix the mixture with 1-5% of the weight of the mixture of lanolin alcohol and stir to obtain the first material; (2) Add the seaweed extract to water at 60-70°C at 10-20 times its weight in water and stir to obtain the second material; (3) The first material and the second material are mixed and added to a high-shear reactor for high-shear reaction, and then filtered to obtain the third material; (4) The third material is placed together with the surfactant, foaming agent, thickener, lubricant, humectant and water in a homogenizer and homogenized to obtain the shampoo.

2. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The preparation method of the extract of *Gentiana macrocarpa* is as follows: after crushing the tuberous root of *Gentiana macrocarpa*, it is added to an ethanol solution with a volume of 10-20 times and extracted for 10-20 hours. The extraction is repeated 2-3 times. The extracts are combined and concentrated to half of the original volume to obtain the extract.

3. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The preparation method of the cardamom extract is as follows: take dried mature cardamom seeds and crush them into powder. Add 10-15 times the weight of water and 0.1-0.5% cellulase to the powder and treat for 2-4 hours. Then add saturated lime water solution for ultrasonic extraction 2-3 times. Combine the extracts and adjust the pH to neutral. Then add it to macroporous resin for adsorption and elute with ethanol solution. Let the eluent crystallize and crush it to obtain the extract.

4. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The Platycladus orientalis leaf extract is composed of Platycladus orientalis leaf volatile oil components and Platycladus orientalis leaf water-soluble components in a volume ratio of 1:50-100.

5. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The seaweed extract is prepared as follows: First, the seaweed is pulverized and soaked in hydrochloric acid with a mass concentration of 10-20% for 3-4 days. After removing the acid, it is washed with water until neutral. Then, a sodium hydroxide solution with a mass concentration of 10-15% is added and heated to boiling for 1-2 hours. After removing the alkali, it is washed with water until neutral. Next, a sodium hydroxide solution with a mass concentration of 40-55% is added and microwaved for 10-20 minutes. After removing the alkali, it is washed with water until neutral, dried, and pulverized to obtain the extract.

6. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The surfactant is any one or a combination of two of lauryl glucoside, lauryl ether ammonium sulfate, cocoyl hydrolyzed oat protein potassium, and polyethylene glycol stearate.

7. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The foaming agent is alkylolamide or cocamidopropyl betaine; the thickener is one of xanthan gum, guar gum or hydroxyethyl cellulose.

8. The method for preparing a traditional Chinese medicine shampoo that can induce hair follicle regeneration and promote hair growth according to claim 1, characterized in that, The lubricant is silicone oil; the humectant is propylene glycol or sodium hyaluronate.

Images