A cell remover for purifying cultured cells

Abstract

The present invention discloses a cell remover for purifying cultured cells, comprising a knife handle and a blade, and also comprising a telescopic device, wherein the knife handle is composed of a rod body and a handle grip which cooperate with each other, and the handle grip comprises a left handle and a right handle; the left handle is connected to the right handle in cooperation with each other through the telescopic device; the left end of the right handle cooperates with the right end of an inner shell in the telescopic device and is connected in an integrated manner; the right end of the left handle cooperates with the left end of an outer shell in the telescopic device and is connected in an integrated manner, and the left end of the left handle is wrapped around the right end of the rod body; the left end of the rod body is connected in an integrated manner with the right end of the blade, so as to form a cell remover for purifying cultured cells, which can remove single or multiple impurity cells under a microscope in the early stage of primary cell culture without causing harm to other normal cells, thereby shortening the culture cycle, achieving high purification efficiency, and being sterile and simple.

Description

Technical Field

[0001] The invention relates to the technical field of laboratory tools, in particular to a single foreign cell remover used in the extraction and purification of primary cells. Background Art

[0002] At present, the purification methods of primary adherent cells are as follows: (I) Natural purification method: Since primary cells have strong proliferation ability, they can be cultured alone by long-term multi-generation biological methods to purify a certain type of target cells through natural optimization. However, the disadvantage of this method is that it is impossible to freely select the cells to be cultured according to experimental requirements. Moreover, the culture cycle is relatively long; (ii) Artificial purification method: 1) Enzyme digestion method: Since the tolerance of cells to trypsin is different, the enzyme digestion method can effectively separate the two types of cells. However, if the time control of this method is not appropriate, it will affect the cells and be detrimental to cell growth; 2) Repeated adhesion method: Purify cells by using the difference in the adhesion ability of different cells; 3) Cloning method: Separation of different subpopulations of the same cell line; 4) Culture medium definition method: Certain cells must have or must remove certain substances during their growth, otherwise they will not be able to grow. This technology is used to purify cells; 5) Flow cytometer separation method: Cells can be separated into different groups based on parameters such as cell size and cell surface markers; 6) Mechanical scraping method: When culturing cells to adhere to the wall, different types of cells tend to grow in patches, so cell scraping can be used to remove non-target cell areas, but the disadvantage of this treatment method is that the culture cycle is long and it cannot be burned under an alcohol lamp, so it cannot be effectively sterilized; in addition, the scraper head is too soft and spacious to remove single impurity cells from the adherent cells.

[0003] The existing cell scraper has a structure including a handle and a blade, wherein: (1) the blade is made of thermoplastic elastomer material TPE, and the handle is made of PP or ABS; (2) the blade head is made of elastic material and is soft to the touch; (3) the blade head is set at a certain angle and is spacious, which is mainly suitable for harvesting cells and is convenient for mechanically harvesting adherent cells from culture containers. However, the existing cell scraper has the following disadvantages: (1) the handle and blade head of the cell scraper are made of PP and TPE respectively, which cannot be burned under an alcohol lamp and cannot be effectively sterilized; (2) the cell scraper head is too soft and spacious, and cannot remove single impurity cells from adherent cells.

[0004] Therefore, a cell remover for culture cell purification is specially invented, which can remove single or multiple impurity cells in the early stage of primary cell culture without harming other normal cells, shortening the culture cycle, with high purification efficiency, sterility and simplicity. Summary of the invention

[0005] Aiming at the deficiencies of the above-mentioned existing technologies, the present invention aims to provide a cell remover for culturing cell purification, which can remove single or multiple impurity cells at the initial stage of primary cell culture, cause no harm to other normal cells, shorten the culture cycle, have high purification efficiency, and be sterile and simple.

[0006] To solve the above technical problems, the present invention adopts the following technical solutions:

[0007] The present invention provides a cell remover for culturing cell purification, including a knife handle and a blade, and further including a telescopic device. The knife handle is composed of a rod body and a handle grip cooperating with each other, and the handle grip includes a left handle and a right handle; the left handle is connected to the right handle through the telescopic device; the left end of the right handle is integrally connected to the right end of the inner shell in the telescopic device; the right end of the left handle is integrally connected to the left end of the outer shell in the telescopic device, and the left end of the left handle is wrapped around the right end of the rod body; the left end of the rod body is integrally connected to the right end of the blade, forming a cell remover for culturing cell purification.

[0008] Preferably, the inner shell in the telescopic device is placed inside the outer shell, and the outer shell is movably connected to the inner shell; the telescopic device is internally provided with a spring clip and two limit blocks, and the two limit blocks are mirror-symmetrically arranged on both sides of the extension end of the spring clip respectively; the spring clip is arranged on the inner side of the inner shell, and a slot hole for slidably connecting with the limit block is symmetrically opened on the upper and lower sides of the inner shell respectively, for facilitating the retraction or extension of the limit block; a plurality of grooves are symmetrically opened on the upper and lower sides of the outer shell respectively, to cooperate with the top of the limit block for snap connection, so as to be used for elongation or shortening, and to prevent the inner shell from falling off.

[0009] Preferably, the knife handle is made of a metal material, and the rod body in the knife handle is in the shape of a slender rod. The handle grip is provided with a frosted texture for increasing the friction force, facilitating the hand to hold tightly during operation and not easy to slide, and at the same time, the knife handle can be placed under an alcohol lamp for burning sterilization.

[0010] Preferably, the front end of the blade body of the blade is provided with a knife head, and the side end of the blade body of the blade is provided with a side knife head, which are combined into a blade in the shape of "卜", and the length of the knife head is greater than the length of the side knife head to prevent other cells from being damaged when removing impurity cells; the side of the knife head is set in the shape of a blade edge, which is beneficial to removing impurity cells in each corner.

[0011] Preferably, the blade is set in a thin sheet shape for conveniently bending and adjusting the blade angle to select a suitable angle to remove impurity cells in each corner; the knife head and the side knife head are made of platinum material or nickel-chromium alloy material; the blade body of the blade is made of stainless steel material, and the blade and the blade body can be placed under an alcohol lamp for burning sterilization to perform effective sterilization.

[0012] The beneficial effects of the present invention are: the cell remover for purifying cultured cells in the present invention can remove single or multiple impurity cells under a microscope in the early stage of primary cell culture without harming other normal cells, shortening the culture cycle, and having high purification efficiency, sterility and simplicity. Specifically:

[0013] (1) The scraper is made of platinum or nickel-chromium alloy, can be bent to adjust the angle, and can be placed under an alcohol lamp to burn for effective sterilization;

[0014] (2) The scraper is divided into two parts: the front blade and the side blade. The front blade is longer than the side blade to prevent other cells from being damaged when removing impurity cells. Both parts are very narrow, which is conducive to the removal of impurity cells in every corner.

[0015] (3) The handle is made of metal, which can be sterilized by burning under an alcohol lamp, and is frosted to increase friction;

[0016] (4) The handle adopts a telescopic device, which can adjust the length of the handle and is suitable for removing cells from culture dishes and cell culture bottles of any size. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 A schematic diagram of the structure of a cell remover for purifying cultured cells provided in an embodiment of the present invention;

[0019] Figure 2 for Figure 1 A partial enlarged view of the middle A part;

[0020] Figure 3 for Figure 1 Schematic diagram of the changing state of

[0021] Figure 4 A schematic diagram of the telescopic device.

[0022] Description of reference numerals:

[0023] Handle 1, blade 2, telescopic device 3;

[0024] Rod body 101, left handle 102, right handle 103;

[0025] Cutting head 201, side cutting head 202;

[0026] Inner shell 301 , outer shell 302 , spring clip 303 , and limit block 304 . DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example 1

[0028] like Figures 1 to 4 As shown, a cell remover for culture cell purification includes a handle 1 and a blade 2, and also includes a telescopic device 3, wherein the handle 1 is composed of a rod body 101 and a handle handle, and the handle handle includes a left handle 102 and a right handle 103; the left handle 102 is connected to the right handle 103 through the telescopic device 3; the left end of the right handle 103 is connected to the right end of the inner shell 301 in the telescopic device 3; the right end of the left handle 102 is connected to the left end of the outer shell 302 in the telescopic device 3; the left end of the left handle 102 is wrapped on the right end of the rod body 101; the left end of the rod body 101 is connected to the right end of the blade 2 to form a cell remover for culture cell purification. Among them, the telescopic device is used to adjust the length of the handle, which is suitable for removing cells in culture dishes and cell bottles of any size.

[0029] Further, in an embodiment, Figure 4 As shown, the inner shell 301 in the telescopic device 3 is built into the outer shell 302, and the outer shell 302 is movably connected to the inner shell 301; the telescopic device 3 is built with a spring clip 303 and two limit blocks 304, and the two limit blocks 304 are respectively placed on both sides of the extended end of the spring clip 303 in a mirror-symmetrical manner; the spring clip 303 is arranged on the inner side of the inner shell 301, and the upper and lower sides of the inner shell 301 are symmetrically provided with a slot hole that cooperates with the limit block 304 for sliding connection, so as to facilitate the retraction or extension of the limit block; the upper and lower sides of the outer shell 301 are symmetrically provided with a plurality of grooves that cooperate with the top of the limit block 304 for interlocking connection, so as to be extended or shortened for use, so as to prevent the inner shell from falling off.

[0030] Further, such as Figure 1 As shown, the knife handle 1 is made of metal material, and the rod body 101 in the knife handle 1 is in the shape of a slender rod. The handle is provided with frosted texture to increase friction, so that the hand can hold it tightly and it is not easy to slide during operation. At the same time, the knife handle can be placed under an alcohol lamp for burning and sterilization.

[0031] Further, such as Figure 1and Figure 2 As shown, a cutting head 201 is provided at the front end of the blade body of the blade 2, and a side cutting head 202 is provided at the side end of the blade body of the blade. The combination forms a blade in the shape of a "bu" character, and the length of the cutting head 201 is greater than the length of the side cutting head 202 to prevent other cells from being damaged when removing impurity cells; the side surface of the cutting head is arranged in a blade shape to facilitate the removal of impurity cells in each corner.

[0032] Furthermore, the blade 2 is arranged in a thin sheet shape to facilitate bending and adjusting the angle of the blade to select a suitable angle to remove impurity cells in each corner; the cutting head 201 and the side cutting head 202 are made of platinum material or nickel-chromium alloy material; the blade body in the blade is made of stainless steel material, and the blade and the blade body can be placed under an alcohol lamp for burning sterilization to perform effective sterilization treatment.

[0033] Working principle: The handle is mainly made of metal material, which is firm and not easy to bend. It is also attached with frosting to increase the friction force, facilitating the hand to hold tightly during operation; the handle is provided with a telescopic device to adjust the length, which is suitable for removing cells in petri dishes and cell bottles of any size; the scraper is arranged in a thin sheet shape and can be adjusted at will, the cutting head and the side cutting head are made of platinum or nickel-chromium alloy, and can be sterilized under an alcohol lamp; at the same time, the cutting head is longer than the side cutting head to prevent other cells from being damaged when removing impurity cells; the cutting head and the side cutting head are narrow in width to facilitate the removal of impurity cells in each corner; and the whole cell remover is made of metal material, which is convenient to be placed under an alcohol lamp for burning to perform effective sterilization treatment.

[0034] In addition, it should be noted that when removing impurity cells, it must be observed under a microscope in a sterile environment.

[0035] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these changes and modifications.

Claims

1. A cell remover for purifying cultured cells, characterized in that: It includes a knife handle and a blade, and also includes a telescopic device. The knife handle is composed of a rod body and a handle grip that cooperate with each other, and the handle grip includes a left handle and a right handle; the left handle is connected to the right handle through the telescopic device; the left end of the right handle is integrally connected to the right end of the inner shell in the telescopic device; the right end of the left handle is integrally connected to the left end of the outer shell in the telescopic device, and the left end of the left handle is wrapped around the right end of the rod body; the left end of the rod body is integrally connected to the right end of the blade, forming a cell remover for culturing cell purification; a knife head is provided at the front end of the blade body of the blade, and a side knife head is provided at the side end of the blade body of the blade, and they are combined into a "卜”-shaped blade, and the length of the knife head is greater than the length of the side knife head; the blade is set in a thin sheet shape; the knife head and the side knife head are made of platinum material or nickel-chromium alloy material; the blade body in the blade is made of stainless steel material.

2. A cell remover for purifying cultured cells as claimed in claim 1, characterized in that: The inner shell in the telescopic device is placed inside the outer shell, and the outer shell is movably connected to the inner shell; the telescopic device is internally provided with a spring clip and two limit blocks, and the two limit blocks are mirror-symmetrically placed on both sides of the extension end of the spring clip respectively; the spring clip is arranged on the inner side of the inner shell, and a slot hole that is slidably connected with the limit block is symmetrically opened on the upper and lower sides of the inner shell respectively; a plurality of grooves are symmetrically opened on the upper and lower sides of the outer shell respectively to be engaged with the top of the limit block for elongation or shortening use.

3. A cell remover for purifying cultured cells as claimed in claim 1, characterized in that: The knife handle is made of metal material, and the rod body in the knife handle is in the shape of a slender rod, and the handle grip is provided with a matte texture.

Images