A method for preparing high purity ecdysone from morningglory

Abstract

The present application relates to a kind of methods for preparing high-purity ecdysone from dew grass, comprising (1) pretreatment, (2) enzymolysis, the complex enzyme includes cellulase, pectinase, papain and bromelain;(3) extraction, (4) purification: dew grass extract is passed through macroporous adsorption resin, sequentially using alkali washing, one water washing, acid washing, twice water washing, ethanol elution, concentration;(5) bleaching, (6) crude crystallization, (7) recrystallization.The purity and yield of β-ecdysone product obtained by the method of the present application are high, and the composite enzyme enzymolysis is combined with ultrasonic countercurrent extraction, which can efficiently and quickly extract;During the purification process, low-concentration alkali washing and acid washing can effectively elute impurities, improve the purity of β-ecdysone, and also reduce the difficulty of macroporous resin regeneration, improve production efficiency.The method of the present application is simple, and the resin and solvent used can be reused, with low process cost, suitable for industrial production.

Description

Technical Field

[0001] This invention relates to the field of natural product extraction, specifically to a method for preparing high-purity ecdysone from dewgrass. Background Technology

[0002] Dewdrop is a perennial, spreading herb that grows on grassy mountain slopes. In my country, it is mainly distributed in Shaanxi, Gansu, Jiangxi, Henan, Guangxi, Sichuan, Guizhou, and Yunnan provinces. The roots of dewdrop are used medicinally, harvested in summer and autumn (washed and used fresh or dried). It has a sweet taste and neutral properties, and is used to relax muscles and tendons, replenish deficiencies and eliminate dampness, reduce deficiency heat, regulate menstruation, and relieve pain. Dewdrop is one of the richest medicinal plants in nature in terms of phytosterols, with β-ecdysone being its main active ingredient.

[0003] β-ecdysone, molecular formula C 27 H 44 O7 has a molecular weight of 486.64.

[0004]

[0005] β-ecdysone has a broad market. Multiple safety studies have shown that it has no side effects, low toxicity, and does not interact with hormone production. It possesses strong pharmacological activity: in the human body, it promotes collagen synthesis, combats arrhythmia, and relieves fatigue; it promotes cell growth, stimulates dermal cell division, and eliminates cholesterol; it lowers blood lipids and inhibits blood sugar rise; it promotes blood circulation, removes dampness, and relieves pain; it is also a natural anti-cancer agent. In sericulture, ecdysone is used to shorten the silkworm's instar time, ensure uniform cocooning, and promote silk spinning. In aquaculture, ecdysone is essential for the growth, development, molting, and metamorphosis of aquatic crustaceans—shrimp and crabs. It is a major raw material for "molting hormones," enabling shrimp and crabs to molt smoothly, promoting uniform molting, effectively preventing cannibalism, and significantly improving survival rates and marketable size. In the cosmetics industry, it is an effective substance for enhancing cell metabolism and activation, and has good exfoliating, spot-removing and whitening effects. It has a good repairing effect on facial melasma, traumatic dark spots, freckles, and melanin deposition, and also has a significant effect on acne.

[0006] CN112375119A discloses a method for separating and purifying high-purity ecdysone. Using *Desmodium styracifolium* root powder as a precipitating agent, an inducer is added, and the mixture is separated by high-performance liquid chromatography (HPLC). Then, a magnetic nanoparticle clarifying agent is used for adsorption to obtain high-purity ecdysone. However, the magnetic nanoparticle clarifying agent has a complex preparation process, is difficult to recycle, and is only suitable for laboratory preparation, not industrial production.

[0007] CN114751951A discloses a method for the large-scale preparation of β-ecdysone from *Desmodium styracifolium*. The method involves first soaking whole *Desmodium styracifolium* powder in alkaline water, followed by extraction with ethyl acetate and activated carbon. After acidification of the extract, impurities are filtered through diatomaceous earth, and the filtrate is concentrated into an extract. The extract is dissolved in an acetone-dichloromethane solution, concentrated, and initially crystallized. The crystals are then washed with an organic solvent (petroleum ether, n-hexane, or dichloromethane), and finally recrystallized after hot melting with methanol or ethanol. The resulting ecdysone product has a purity of over 98%. However, this method involves acetone and ethyl acetate, which are flammable and volatile solvents; and dichloromethane, a Group 2A carcinogen. This poses a risk of harm to the environment and operators. Furthermore, the methanol recrystallization process results in significant solvent residue, limiting the product's applicability to veterinary applications and making it unsuitable for higher-requirement pharmaceutical and cosmetic uses.

[0008] CN105440095A discloses a dewwort extract rich in β-ecdysone and its preparation method. The method involves using dewwort root as dewwort powder, followed by water extraction, alcohol precipitation, resin adsorption, elution, and recrystallization to obtain a dewwort extract containing 87.8-90.0% β-ecdysone. This process suffers from high alcohol consumption, low β-ecdysone content, a recovery rate of only 52%-62%, and high production costs, making it unsuitable for large-scale production.

[0009] CN101735299A discloses a method for extracting ecdysone from *Desmodium styracifolium*. *Desmodium styracifolium* powder is extracted by boiling in water or by extraction with methanol or ethanol, adjusting the pH to 6-10, and then extracted with a mixed solvent of n-butanol and ethyl acetate. The extract is concentrated, separated using a strong-base anion exchange resin, and then eluted, concentrated under reduced pressure, and dried to obtain ecdysone. This process requires mixed solvent extraction and ion exchange resin purification to achieve a ecdysone purity of 93%. Furthermore, it uses n-butanol, which has acute toxicity, and ethyl acetate, which is volatile and flammable. This places high demands on the production environment, poses potential safety hazards to personnel, and results in high production costs, making it unsuitable for large-scale production.

[0010] Currently, the most economically valuable application of β-ecdysone is in cosmetics. However, the cosmetic industry has very high requirements for the purity of active ingredients. Existing technologies for preparing β-ecdysone from *Hydrocotyle vulgaris* extract cannot simultaneously achieve high purity and high yield, generally limiting the purity to around 95%. Further improvements in purity require multi-step crystallization or even methods using large-scale equipment such as HPLC, which constitutes a limitation for large-scale industrial production of β-ecdysone from *Hydrocotyle vulgaris* for use in cosmetics. Summary of the Invention

[0011] To overcome the aforementioned deficiencies in the existing technology, this invention provides a method for preparing high-purity ecdysone from *Hymenochloa crus-galli*, resulting in a product with high purity and high yield. The technical solution adopted by this invention to solve its technical problem is as follows:

[0012] A method for preparing high-purity ecdysone from dewwort includes the following steps:

[0013] (1) Pretreatment: Select dry dew grass that is free from rot, mold and visible impurities, and crush it to obtain dew grass powder;

[0014] (2) Enzymatic hydrolysis: Disperse the dew grass powder obtained in step (1) in water, adjust the pH to weakly acidic, and perform enzymatic hydrolysis with a compound enzyme to obtain the hydrolysate;

[0015] Preferably, in step (2), the mass ratio of dew grass powder to water is 1:20-30, the pH is adjusted to be slightly acidic to 6-6.5, the compound enzyme is a mixture of cellulase, pectinase, papain and bromelain in a mass ratio of 6-10:3-5:1.4-2.2:1-1.6, the amount of compound enzyme is 0.6-0.9 wt% of the mass of dew grass powder, and the enzymatic hydrolysis is carried out at 40-50℃ for 2-3 hours;

[0016] (3) Extraction: The enzymatic hydrolysate was subjected to continuous ultrasonic countercurrent extraction and centrifugation to obtain the dew grass extract;

[0017] The conditions for continuous ultrasonic countercurrent extraction are: extraction temperature 40-70℃, ultrasonic power 200-300W, ultrasonic frequency 25-60kHz, and continuous countercurrent ultrasonic extraction time 2-5h.

[0018] (4) Purification: The dew grass extract in step (3) is passed through a macroporous adsorption resin and successively washed with alkali, washed with water once, washed with acid, washed with water twice, and eluted with ethanol. The ethanol eluent is concentrated under reduced pressure until it is alcohol-free to obtain an eluent rich in β-ecdysone.

[0019] Preferably, in step (4), the macroporous adsorption resin is of the following types: D101, AB-8, LX-T28, etc.

[0020] Preferably, in step (4), the alkaline washing is a 0.2-0.5 wt% sodium hydroxide solution, with a dosage of 1.5-2.5 BV; the water washing alkali dosage is 2-3 BV; the acid washing is a 0.5-1 wt% citric acid or 0.1-0.2 wt% hydrochloric acid solution, with a dosage of 1-1.5 BV; the water washing acid endpoint is when the pH of the effluent is neutral; the ethanol concentration is 70-80 vol%, with a dosage of 2-3 BV.

[0021] (5) Decolorization: The eluent concentrate in step (4) is first decolorized by ion exchange resin, and then activated carbon is added to the effluent of ion exchange resin. The mixture is kept at 70°C and stirred. After solid-liquid separation, a decolorized solution rich in β-ecdysone is obtained.

[0022] Preferably, in step (5), the ion exchange resin is of the type D941, LX-94, LX-T5, LXD-762, etc.

[0023] Preferably, in step (5), the amount of activated carbon added is 5-10% of the weight of the solids in the effluent; the heat preservation time is 2-4 hours.

[0024] (6) Crude crystallization: The decolorizing solution obtained in step (5) is concentrated to obtain a decolorizing concentrate. The decolorizing concentrate is cooled and stirred at low speed to crystallize, and the crude product is separated.

[0025] Preferably, in step (6), the solid concentration of the decolorizing concentrate is 25-40%, the concentration temperature is 65-75℃, and the crystallization temperature is 20-25℃.

[0026] (7) Recrystallization: The crude product obtained in step (6) is heated with an aqueous ethanol solution until completely dissolved, cooled and stirred at low speed to crystallize, the recrystallized crystals are separated, and after drying, the product β-ecdysone is obtained.

[0027] Preferably, in step (7), the concentration of the ethanol aqueous solution is 30-50%, and the ratio of the amount of ethanol aqueous solution to the amount of crude crystals is 2-3L:1kg.

[0028] Preferably, in step (7), the heating and dissolution temperature is 70-80℃, and the recrystallization temperature is -10 to 0℃.

[0029] Compared with the prior art, the present invention has at least the following beneficial effects:

[0030] (1) The β-ecdysone product obtained by the method of the present invention has high purity and yield. In the preferred embodiment, the purity of β-ecdysone is above 97% and the yield is above 80%.

[0031] (2) The present invention uses a combination of compound enzyme hydrolysis and ultrasonic countercurrent extraction, which can extract efficiently and quickly with a high yield;

[0032] (3) In the purification process of the present invention, low-concentration alkali washing and acid washing are used, which can effectively wash away impurities, improve the purity of β-ecdysone, reduce the regeneration difficulty of macroporous resin, and improve production efficiency.

[0033] (4) In the decolorization process of the present invention, the use of ion exchange resin and activated carbon in combination can effectively remove impurities such as pigments and proteins. The concentrated system environment is more conducive to the detachment of ecdysone crystals.

[0034] (5) The main reagents used in the process of the present invention are water and ethanol. The production process is free of toxic volatiles and has high safety. The low temperature and low speed stirring recrystallization method is adopted to obtain β-ecdysone ethanol residue ≤200ppm, and the application of the product is not restricted.

[0035] (6) The method of the present invention has a simple process, and the resin and solvent used in the process can be reused. The process cost is very low and it is suitable for industrial production. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be described in detail below. The following embodiments are provided to better understand this invention, but do not limit the invention. Unless otherwise specified, the experimental methods in the following embodiments are conventional methods.

[0037] The dried dew-covered grass used in this invention contains 2.29 wt% β-ecdysone.

[0038] Example 1

[0039] (1) Take 1000g of dried dew grass that is free from rot, mold, and visible impurities, crush it, and take the material that passes through a 10-mesh sieve.

[0040] (2) Enzymatic hydrolysis: Disperse the dew grass powder obtained in step (1) in water at a solid-liquid ratio of 1:30 (w / w), adjust the pH to 6.5 with dilute hydrochloric acid, add 6g of compound enzyme (a mixture of cellulase, pectinase, papain and bromelain in a mass ratio of 10:5:1.4:1.6), and enzymatically hydrolyze at 40℃ for 3h to obtain the enzymatic hydrolysate;

[0041] (3) The enzymatic hydrolysate was continuously extracted with 10L of water using countercurrent ultrasonic extraction for 4 hours. The ultrasonic power was 200W and the frequency was 25kHz. After extraction, the extract was filtered and centrifuged to obtain the extract.

[0042] (4) Pass the extract obtained in step (3) through D101 macroporous adsorption resin at a flow rate of 1 BV / h, and wash with 2 BV 0.3 wt% sodium hydroxide solution, 2 BV pure water, 1 BV 1 wt% citric acid solution, pure water until neutral, and 3 BV 70 vol% ethanol at a flow rate of 1 BV / h. Collect the ethanol eluent and concentrate it under reduced pressure until alcohol-free.

[0043] (5) Pass the concentrated ethanol eluent obtained in step (4) through D941 ion exchange resin at a flow rate of 0.5 BV / h, and then wash the column with 2 BV of pure water at a flow rate of 1 BV / h. Mix the collected D941 ion exchange resin column effluent and water washing liquid, add 5% of activated carbon by weight of the solids in the solution, keep warm at 70℃ and stir for 2 hours, and obtain β-ecdysone decolorizing solution by solid-liquid separation.

[0044] (6) The β-ecdysone decolorizing solution obtained in step (5) is concentrated under reduced pressure to a solid concentration of 30%, and the solution is stirred at low speed at room temperature to carry out initial crystallization and separate the initial crystals.

[0045] (7) Add 3 times the volume of 50% ethanol to the initial crystallized crystals obtained in step (6), heat to 70°C to completely dissolve them, stir at low speed at -10°C to crystallize, separate the recrystallized crystals, and dry them to obtain 20.18g of ecdysone product with a purity of 98.42% and ethanol residue <200ppm. The calculated yield of β-ecdysone is 86.73%.

[0046] Example 2

[0047] (1) Take 1000g of dried dew grass that is free from rot, mold, and visible impurities, crush it, and take the material that passes through a 10-mesh sieve.

[0048] (2) Enzymatic hydrolysis: Disperse the dew grass powder obtained in step (1) in water at a solid-liquid ratio of 1:40 (w / w), adjust the pH to 6 with dilute hydrochloric acid, add 9g of compound enzyme (a mixture of cellulase, pectinase, papain and bromelain in a mass ratio of 6:3:2.2:1), and enzymatically hydrolyze at 40℃ for 4h to obtain the enzymatic hydrolysate.

[0049] (3) At 50℃, 1000g of the sieve material obtained in step (2) was continuously extracted by countercurrent ultrasonic extraction with 10L of water for 3h, wherein the ultrasonic power was 200W and the frequency was 25kHz. After extraction, the mixture was filtered and centrifuged to obtain the extract.

[0050] (4) Pass the extract obtained in step (3) through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, and wash with 2 BV 0.5 wt% sodium hydroxide solution, 2 BV pure water, 1.5 BV 0.2 wt% hydrochloric acid solution, pure water until neutral, and elute with 2.5 BV 80 vol% ethanol at a flow rate of 1 BV / h. Collect the ethanol eluent and concentrate it under reduced pressure until alcohol-free.

[0051] (5) Pass the concentrated ethanol eluent obtained in step (4) through LX-T5 ion exchange resin at a flow rate of 0.5 BV / h, and then wash the column with 2 BV of pure water at a flow rate of 1 BV / h. Mix the collected LX-T5 ion exchange resin column effluent and water washing liquid, add 5% of activated carbon by weight of the solids in the solution, keep warm at 70℃ and stir for 2 hours, and obtain β-ecdysone decolorizing solution by solid-liquid separation.

[0052] (6) The β-ecdysone decolorizing solution obtained in step (5) is concentrated under reduced pressure to a solid concentration of 40%, and the solution is stirred at low speed at room temperature to carry out initial crystallization and separate the initial crystals.

[0053] (7) Add 3 times the volume of 30% ethanol to the initial crystallized crystals obtained in step (6), heat to 70°C to completely dissolve them, stir at low speed at -10°C to crystallize, separate the recrystallized crystals, and after drying, obtain 20.95g of ecdysone product with a purity of 97.21% and ethanol residue <200ppm. The calculated yield of β-ecdysone is 88.93%.

[0054] Example 3

[0055] The other conditions were the same as in Example 1, except that in step (2), the complex enzyme was a mixture of cellulase, pectinase, papain, and bromelain in a mass ratio of 2:1:1:1. A final yield of 20.04g of ecdysone product with a purity of 95.87% and ethanol residue <200ppm was obtained, with a calculated yield of 83.90% for β-ecdysone.

[0056] Comparative Example 1

[0057] The other conditions were the same as in Example 1, except that in step (2), the complex enzyme was a mixture of pectinase, papain, and bromelain in a mass ratio of 5:1.4:1.6, i.e., cellulase was not added. The final product yielded 18.57g of ecdysone with a purity of 96.63% and ethanol residue <200ppm. The calculated yield of β-ecdysone was 78.36%.

[0058] Comparative Example 2

[0059] Other conditions were the same as in Example 1, except that in step (2), the complex enzyme was a mixture of cellulase, papain, and bromelain in a mass ratio of 10:1.4:1.6, i.e., no pectinase was added. The final product yielded 18.92g of ecdysone with a purity of 95.90% and ethanol residue <200ppm, resulting in a calculated β-ecdysone yield of 79.23%.

[0060] Comparative Example 3

[0061] Other conditions were the same as in Example 1, except that in step (2), the complex enzyme was a mixture of cellulase, pectinase, and bromelain in a mass ratio of 10:5:1.6, i.e., papain was not added. The final product yielded 19.05g of ecdysone with a purity of 96.48% and ethanol residue <200ppm, resulting in a calculated β-ecdysone yield of 80.05%.

[0062] Comparative Example 4

[0063] Other conditions were the same as in Example 1, except that in step (2), the complex enzyme was a mixture of cellulase, pectinase, and papain in a mass ratio of 10:5:1.4, i.e., bromelain was not added. The final product yielded 18.84g of ecdysone product with a purity of 96.08% and ethanol residue <200ppm. The calculated yield of β-ecdysone was 79.04%.

[0064] Comparative Example 5

[0065] The other conditions were the same as in Example 1, except that in step (4), after the extract was passed through the D101 macroporous adsorption resin at a flow rate of 1 BV / h, it was washed sequentially with 1 BV of 1 wt% citric acid solution and pure water until neutral, and then eluted with 3 BV of 70 vol% ethanol at a flow rate of 1 BV / h. The ethanol eluent was collected and concentrated under reduced pressure until alcohol-free. This eliminated the need for the alkaline washing step. Finally, 21.78 g of ecdysone product with a purity of 87.04% and ethanol residue <200 ppm was obtained, and the calculated yield of β-ecdysone was 82.78%.

[0066] Comparative Example 6

[0067] Other conditions were the same as in Example 1, except that in step (4), the extract was washed sequentially with 2 BV of 0.3 wt% sodium hydroxide solution and pure water until neutral at a flow rate of 1 BV / h, and then eluted with 3 BV of 70 vol% ethanol. The ethanol eluent was collected and concentrated under reduced pressure until no alcohol remained. This eliminated the need for acid washing. The final product yielded 19.47 g of ecdysone with a purity of 91.26% and ethanol residue <200 ppm. The calculated yield of β-ecdysone was 77.59%.

[0068] Comparative Example 7

[0069] The other conditions were the same as in Example 1, except that in step (4), the extract was washed sequentially with 1BV of 1wt% citric acid solution, 2BV of pure water, 2BV of 0.3wt% sodium hydroxide solution, and pure water until neutral at a flow rate of 1BV / h, and then eluted with 3BV of 70 vol% ethanol. The ethanol eluent was collected and concentrated under reduced pressure until alcohol-free. That is, the order of acid washing and alkali washing was reversed. Finally, 20.78g of ecdysone product with a purity of 93.16% and ethanol residue <200ppm was obtained, and the calculated yield of β-ecdysone was 84.53%.

[0070] Comparative Example 8

[0071] (1) Take 1000g of dried dew grass that is free from rot, mold, and visible impurities, crush it, and take the material that passes through a 10-mesh sieve.

[0072] (2) At 70℃, 1000g of the sieve material obtained in step (1) was continuously extracted by countercurrent ultrasonic extraction with 10L of water for 2 hours, wherein the ultrasonic power was 200W and the frequency was 25kHz. After extraction, the mixture was filtered and centrifuged to obtain the extract.

[0073] (3) Pass the extract obtained in step (2) through LX-T28 macroporous adsorption resin at a flow rate of 1 BV / h, and wash it sequentially with 2 BV 0.5 wt% sodium hydroxide solution, 2 BV pure water, 1.5 BV 0.2 wt% hydrochloric acid solution, pure water until neutral, and elute with 3 BV 80% ethanol at a flow rate of 1 BV / h. Collect the ethanol eluent and concentrate it under reduced pressure until alcohol-free.

[0074] (4) Pass the concentrated ethanol eluent obtained in step (3) through LXD-762 ion exchange resin at a flow rate of 0.5 BV / h, and then wash the column with 2 BV of pure water at a flow rate of 1 BV / h. Mix the collected LXD-762 ion exchange resin column effluent and water wash solution, add 8% of the weight of activated carbon of the solution solids, keep warm at 70℃ and stir for 2 h, and obtain β-ecdysone decolorizing solution after solid-liquid separation.

[0075] (5) The β-ecdysone decolorizing solution obtained in step (4) is concentrated under reduced pressure to a solid concentration of 30%, and the solution is stirred at low speed at room temperature to carry out initial crystallization and separate the initial crystals.

[0076] (6) Add 50% ethanol, twice the volume of the initial crystallized crystals obtained in step (5), heat to 80°C to completely dissolve it, stir at low speed at -5°C to crystallize, separate the recrystallized crystals, and after drying, obtain 18.44g of ecdysone product with a purity of 97.01% and ethanol residue <200ppm. The calculated yield of β-ecdysone is 78.12%.

[0077] The present invention has been described in detail above with reference to the embodiments. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A method for preparing high-purity ecdysone from dew-covered grass, characterized in that, Includes the following steps: (1) Pretreatment: Select dry dew grass that is free from rot, mold and visible impurities, and crush it to obtain dew grass powder; (2) Enzymatic hydrolysis: Disperse the dew grass powder obtained in step (1) in water, adjust the pH to weakly acidic, and perform enzymatic hydrolysis with a compound enzyme to obtain the hydrolysate; the compound enzyme includes cellulase, pectinase, papain and bromelain. (3) Extraction: The enzymatic hydrolysate was subjected to continuous ultrasonic countercurrent extraction and centrifugation to obtain the dew grass extract; (4) Purification: The dew grass extract in step (3) is passed through a macroporous adsorption resin and successively washed with alkali, washed with water once, washed with acid, washed with water twice, and eluted with ethanol. The ethanol eluent is concentrated under reduced pressure until it is alcohol-free to obtain an eluent rich in β-ecdysone. (5) Decolorization: The elution concentrate in step (4) is first decolorized by ion exchange resin, and then activated carbon is added to the effluent of ion exchange resin. The mixture is kept at 70°C and stirred. After solid-liquid separation, a decolorized solution rich in β-ecdysone is obtained. (6) Crude crystallization: The decolorizing solution obtained in step (5) is concentrated to obtain a decolorizing concentrate. The decolorizing concentrate is cooled and stirred at low speed to crystallize, and the crude product is obtained by separation. (7) Recrystallization: The crude product obtained in step (6) is heated with an aqueous ethanol solution until completely dissolved, cooled and stirred at low speed to crystallize, the recrystallized crystals are separated, and after drying, the product β-ecdysone is obtained.

2. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (2), the mass ratio of dew grass powder to water is 1:20-30, the pH is adjusted to be slightly acidic to 6-6.5, the compound enzyme is a mixture of cellulase, pectinase, papain and bromelain in a mass ratio of 6-10:3-5:1.4-2.2:1-1.6, the amount of compound enzyme is 0.6-0.9 wt% of the mass of dew grass powder, and the enzymatic hydrolysis is carried out at 40-50℃ for 2-3 hours.

3. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (3), the conditions for continuous ultrasonic countercurrent extraction are: extraction temperature 40-70℃, ultrasonic power 200-300W, ultrasonic frequency 25-60kHz, and extraction time 2-5h.

4. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (4), the macroporous adsorption resin is of the following types: D101, AB-8 or LX-T28.

5. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (4), the alkaline washing is a 0.2-0.5 wt% sodium hydroxide solution, with a dosage of 1.5-2.5 BV; the water washing alkali dosage is 2-3 BV; the acid washing is a 0.5-1 wt% citric acid or 0.1-0.2 wt% hydrochloric acid solution, with a dosage of 1-1.5 BV; the water washing acid endpoint is when the pH of the effluent is neutral; the concentration of ethanol is 70-80 vol%, with a dosage of 2-3 BV.

6. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (5), the ion exchange resin is of type D941, LX-94, LX-T5 or LXD-762.

7. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (5), the amount of activated carbon added is 5-10% of the weight of the solids in the effluent; the heat preservation time is 2-4 hours.

8. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (6), the solid concentration of the decolorizing concentrate is 25-40%, the concentration temperature is 65-75℃, and the crystallization temperature is 20-25℃.

9. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (7), the concentration of the ethanol aqueous solution is 30-50%, and the ratio of the amount of ethanol aqueous solution to the amount of crude crystal is 2-3L:1kg.

10. The method for preparing high-purity ecdysone from dewwort according to claim 1, characterized in that, In step (7), the heating and dissolution temperature is 70-80℃, and the recrystallization temperature is -10 to 0℃.

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