A fat composition, method of making and use thereof

By combining palm oil with sterol laurate through enzymatic modification, an oil composition was prepared, which solved the problem of low oil absorption efficiency in broiler diets, improved the absorption rate of calcium and phosphorus, reduced the feed conversion ratio, and improved breeding efficiency.

CN117296933BActive Publication Date: 2026-04-24JINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JINAN UNIVERSITY
Filing Date
2023-10-11
Publication Date
2026-04-24

AI Technical Summary

Technical Problem

The absorption efficiency of existing oils in broiler diets is poor, leading to an unbalanced feed conversion ratio and low absorption rates of calcium and phosphorus, which affects breeding efficiency.

Method used

An oil composition was prepared by enzymatically modifying palm oil and compounding it with sterol laurate, which increased the content of saturated fatty acids at the sn-2 position. This composition was then added to broiler diets to improve mineral absorption.

Benefits of technology

It improves the absorption rate of calcium and phosphorus in broilers, reduces the feed conversion ratio, and enhances breeding efficiency.

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Abstract

The present application belongs to the field of food oil and fat, and particularly relates to an oil and fat composition, a preparation method and use thereof. The oil and fat composition comprises sterol laurate and modified palm oil, wherein the mass ratio of the sterol laurate and the modified palm oil is (0.5-3):100. The sterol is subjected to esterification with lauric acid under vacuum to obtain a sterol laurate esterification product; the enzyme-modified palm oil is stirred and mixed with the sterol laurate esterification product to obtain the oil and fat additive suitable for poultry daily ration. The present application firstly utilizes the oil and fat composition prepared by compounding the enzyme-modified palm oil and the sterol laurate esterification product as the oil and fat for poultry daily ration. The oil and fat composition reduces the feed-meat ratio of broiler, improves the lipid absorption rate, and improves the absorption of calcium and phosphorus by poultry.
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Description

Technical Field

[0001] This invention belongs to the field of light industrial oils and fats, and specifically relates to an oil and fat composition, its preparation method, and its uses. Background Technology

[0002] In the animal husbandry industry, the role of adding oils to animal diets includes providing energy and essential fatty acids; improving the absorption of fat-soluble vitamins; slowing down the passage of food through the gastrointestinal tract and improving energy utilization efficiency; and enhancing the body's resistance to heat stress. Among these, energy utilization efficiency is a relatively intuitive and direct factor that affects the industry's profitability, specifically reflected in the feed conversion ratio (FCR). This result varies considerably depending on the type of oil used in feeding.

[0003] For broilers, the feeding cycle is short and individual growth and development are relatively fast. Therefore, in the poultry industry, oils are usually added to increase the energy value of the feed to meet their individual energy needs. However, due to the different absorption efficiencies of oils from different sources, even if the amount of oil added to the feed is the same, the individual's utilization of the feed will vary. Since fat is the nutrient with the highest energy value, a higher oil absorption rate means that less feed is needed to meet the energy requirements for growth and development. Differences in the types, contents, and positions of fatty acids on the glycerol backbone are important factors leading to significant differences in the digestibility and absorption characteristics of different types of oils. Unsaturated fatty acids are more easily absorbed than saturated fatty acids, and saturated fatty acids at the sn-2 position of triglycerides are more easily absorbed than those at the sn-1 and sn-3 positions.

[0004] Meanwhile, broiler diets typically consist of large amounts of corn and soybean meal, which contain phytic acid that easily forms stable complexes with minerals such as calcium and phosphorus, thereby reducing the digestibility and utilization of these minerals. Calcium and phosphorus are the most abundant mineral elements in livestock and poultry, and their intake and absorption are extremely important for the development of broilers. Calcium and phosphorus deficiency can lead to a series of adverse problems in chicks, such as slow growth, loss of appetite, lethargy, poor bone development, joint swelling, weakness, and unsteady gait. Summary of the Invention

[0005] Given that palm oil is widely available, inexpensive, and has low bioavailability, the primary objective of this invention is to provide an enzymatically modified palm oil composition. This invention uses Lipozyme TL IM lipase to catalyze the transesterification reaction of palm oil to obtain an enzymatically modified palm oil product, adds sterol laurate obtained through esterification, and feeds the resulting oil composition to 15-day-old experimental broilers.

[0006] Another object of the present invention is to provide a method for preparing the above-mentioned oil and fat composition.

[0007] Another object of the present invention is to provide the application of the above-mentioned oil composition. It can be used in animal feed to replace traditional oils, improve feed utilization, and enhance the absorption efficiency of minerals by poultry.

[0008] The objective of this invention is achieved through the following solution:

[0009] An enzymatically modified palm oil composition comprising sterol laurate and modified palm oil, wherein the mass ratio of sterol laurate to modified palm oil is (0.5-3):100.

[0010] Preferably, the mass ratio of the sterol laurate to the modified palm oil is (1-3):100, more preferably 2:100.

[0011] The modified palm oil is prepared by enzymatic transesterification of palm oil. The content of saturated fatty acids at the sn-2 position of the modified palm oil is preferably 34-58%, more preferably 47%. The palm oil is commercially available palm oil fractionated at 24°C. The enzyme used is immobilized lipase Lipozyme TL IM, and the amount of Lipozyme TL IM lipase is 0.5-2.5% of the mass of palm oil. The transesterification reaction temperature is 60-80°C, and the reaction time is 15-60 min.

[0012] The modified palm oil is preferably prepared by the following steps: after melting the palm oil, it is pumped into a fixed-bed reactor and reacted with pre-filled Lipozyme TL IM lipase to obtain modified palm oil.

[0013] The sterol laurate is prepared by the following steps: sterol and lauric acid are catalytically esterified under vacuum and high temperature to obtain the sterol laurate product.

[0014] The vacuum condition is preferably an absolute pressure of 3000-5000 Pa, more preferably 4000 Pa;

[0015] The high temperature conditions are preferably 160-200℃, and more preferably 180℃.

[0016] The esterification reaction is a chemical esterification, wherein the catalyst used is concentrated sulfuric acid, and the catalyst addition amount is preferably 0.5-1% of the substrate mass, more preferably 0.7%; the reaction time is preferably 45-80 min, more preferably 60 min.

[0017] The mass ratio of sterol to lauric acid is preferably 0.5:1-1.2:1, and more preferably 0.9:1.

[0018] A method for preparing the above-mentioned oil and fat composition includes the following steps: mixing modified palm oil with sterol laurate to obtain the oil and fat composition.

[0019] The preferred method for stirring and mixing is to heat the mixture to melt and mix the two materials, and more preferably, to mix and stir at 80°C for 40 minutes.

[0020] The above-mentioned oil composition is used as an animal dietary oil in animal feed, especially in broiler feed.

[0021] The above-mentioned oil composition is used as animal dietary oil in animal feed. It is fed by premixing the oil composition into the basal feed. The amount of oil composition is 3-8% of the weight of the basal feed, preferably 5%.

[0022] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0023] The modified palm oil prepared by this invention, when combined with sterol laurate, can be used as a feed oil additive in the livestock industry. Compared with palm oil and modified palm oil, this oil improves the absorption of calcium and phosphorus in the diet of poultry, increases feed utilization, reduces feed costs, and improves breeding efficiency, making it a good choice as a feed oil for poultry. Attached Figure Description

[0024] Figure 1 It is a fixed-bed enzymatic transesterification modification device. Detailed Implementation

[0025] The present invention will be further described in detail below with reference to embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto. Unless otherwise specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments used, unless otherwise specified, are all commercially available conventional products.

[0026] The immobilized lipases used in the examples were all purchased from Novozymes.

[0027] The modified palm oil used in the examples and comparative examples was prepared through the following steps: Commercially available palm oil at 24°C was melted and pumped into a fixed-bed enzymatic transesterification modification device. The palm oil was then fully reacted with Lipozyme TL IM lipase in the device. The maximum filling amount of Lipozyme TL IM lipase was 85g, and the amount of Lipozyme TL IM lipase was 1.2% of the palm oil mass. The samples obtained after the reaction were the modified palm oils (34% modified palm oil with a sn-2 position saturated fatty acid content, with a reaction time of 15 min and a reaction temperature of 60°C; 47% modified palm oil with a sn-2 position saturated fatty acid content, with a reaction time of 30 min and a reaction temperature of 70°C; and 58% modified palm oil with a sn-2 position saturated fatty acid content, with a reaction time of 60 min and a reaction temperature of 80°C).

[0028] The present invention aims to obtain new modified palm oil products by enzymatic transesterification of palm oil, and to obtain new oil compositions by mixing them with sterol laurate. The following examples illustrate the relevant experimental data and comparative test data.

[0029] Example 1

[0030] (1) Take 0.9g of sterol and 1g of lauric acid, heat to melt and mix evenly, then put into a reactor and set the reaction temperature to 180℃. When the temperature is constant, add 0.7% (98%) of concentrated sulfuric acid by weight of the substrate, stir with a magnetic stirrer (500r / min), and carry out the reaction under vacuum conditions (absolute pressure = 4000Pa) with a water circulation pump. After the esterification reaction is completed in 60min, the reaction solution is centrifuged to separate the layers and the oil sample is collected. After washing with water and drying, it is esterified sterol lauric acid.

[0031] (2) An oil composition was obtained by mixing esterified sterol lauric acid with modified palm oil (47% saturated fatty acid content at sn-2 position and 43% UPU content) at a mass ratio of 2:100. The oil composition was added to 5% by weight of the basic feed (mainly corn and soybean meal, formula shown in Table 4) and fed to 15-day-old experimental broilers. The feed and oil were premixed, and the feed was given in a fixed amount every day. The broilers had free access to food and water. Before feeding, the leftover feed in the trough was removed and the weight was recorded.

[0032] Determination of the Sn-2 fatty acid composition: Add approximately 0.1 g of oil sample, 20 mg (porcine pancreatic lipase), and 2 mL of pH 8.0 Tris buffer to a 10 mL centrifuge tube. Gently shake, then add 0.5 mL of 1 g / L sodium cholate solution and 0.2 mL of 220 g / L calcium chloride solution. After completing the above operation within 30 seconds, immediately place the centrifuge tube in a 40°C water bath and shake by hand for 4 minutes. Remove the centrifuge tube from the water bath and vortex vigorously for 1 minute. Then add 1 mL of 6 mol / L HCl and 1 mL of diethyl ether, vortex to mix, and centrifuge to separate the layers. Take the ether layer for thin-layer chromatography analysis (developing solvent: hexane: anhydrous diethyl ether: formic acid = 70:30:1.5). After development, air dry naturally, and develop fluorescence under a 256 nm UV lamp. Scrape off the monoglyceride band, extract with diethyl ether, and determine the fatty acid composition by gas chromatography.

[0033] UPU content determination: UPU content is the percentage of OPO and OPL triglycerides among all triglycerides. Samples were dissolved in a 1:1 mixture of n-hexane and acetone for gas chromatography analysis. Qualitative analysis of triglycerides was then performed using the standard sample method, and quantitative analysis was performed using the area normalization method. Consistent chromatographic conditions were maintained for qualitative analysis, while quantitative analysis was based on the linear relationship between peak area and mass. Analysis was performed using a GC (7820-A, Agilent, Wilmington, DE, USA) equipped with a flame ionization detector and an RTX-65TG capillary column (30m × 0.25mm × 0.1um, RESTEK, USA). Using N2 as the carrier gas, the flow rate was 40 ml / min, the column pressure was 12.379 psi, the injection volume was 1 μL, and the split ratio was 25:1. The initial oven temperature was set to 280 °C and held for 1.5 min, then increased to 340 °C at 10 °C / min and held for 9.5 min, and then increased to 350 °C at 1 °C / min and held for 12 min. The detector and injection port temperatures were both 350 °C.

[0034] Feed conversion ratio (FCR) determination: The feed conversion ratio is the ratio of an individual's feed intake to its weight gain during the feeding period.

[0035] Determination of calcium, phosphorus, and lipid absorption rates: These were determined using the total fecal collection method. The amount absorbed was the intake over a certain period minus the content in the collected feces. Calcium and phosphorus content were determined according to the national standards GB 5009.92-2016 "National Food Safety Standard - Determination of Calcium in Food" and GB 5009.87-2016 "National Food Safety Standard - Determination of Phosphorus in Food". Flame atomic absorption spectrometry was used for calcium content determination, and molybdenum blue spectrophotometry was used for phosphorus content determination. Lipid content was determined by Soxhlet extraction of the collected feces.

[0036] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition in this example is 2.55, the calcium absorption rate is 78.12%, the phosphorus absorption rate is 67.34%, and the lipid absorption rate is 86.65%.

[0037] Example 2

[0038] This embodiment is the same as that in embodiment 1 except for the following technical features: Step (2) is to mix esterified sterol lauric acid (i.e. sterol laurate) with enzyme-modified palm oil (sn-2 position saturated fatty acid content is 47%, UPU content is 43%) at a mass ratio of 0.5:100 to obtain an oil composition. This oil composition is added to 15-day-old experimental broilers at 5% by weight of the basic feed. The feed and oil are premixed, and the feed is given in a fixed amount every day. The broilers can eat and drink freely. Before feeding, the remaining feed in the trough is removed and the weight is recorded.

[0039] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition obtained in this example is 2.64, the calcium absorption rate is 74.26%, the phosphorus absorption rate is 52.45%, and the lipid absorption rate is 78.15%.

[0040] Example 3

[0041] Except for the following technical features, this embodiment is the same as that in embodiment 1: Step (2) is to mix sterol laurate with enzymatically modified palm oil (sn-2 position saturated fatty acid content is 47%, UPU content is 43%) at a mass ratio of 1:100 to obtain an oil composition. This oil composition is added to 15-day-old experimental broilers at 5% by weight of the basic feed. The feed and oil are premixed, and the feed is given in a fixed amount every day. The broilers can eat and drink freely. Before feeding, the leftover feed in the feed trough is removed and the weight is recorded.

[0042] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition obtained in this example is 2.57, the calcium absorption rate is 68.61%, the phosphorus absorption rate is 65.36%, and the lipid absorption rate is 76.43%.

[0043] Example 4

[0044] This embodiment is the same as that in embodiment 1 except for the following technical features: Step (2) mixes sterol laurate with enzymatically modified palm oil (sn-2 position saturated fatty acid content is 47%, UPU content is 43%) at a mass ratio of 3:100 to obtain an oil composition. Add the oil composition to 15-day-old experimental broilers at 5% by weight of the basic feed. The feed and oil are premixed. Feed is given in a fixed amount every day. The broilers can eat and drink freely. Before feeding, the leftover feed in the trough is removed and the weight is recorded.

[0045] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition obtained in this example is 2.61, the calcium absorption rate is 72.83%, the phosphorus absorption rate is 68.52%, and the lipid absorption rate is 82.57%.

[0046] Example 5

[0047] This embodiment is the same as that in embodiment 1 except for the following technical features: Step (2) is to mix sterol laurate with enzymatically modified palm oil (sn-2 position saturated fatty acid content is 34%, UPU content is 28%) at a mass ratio of 2:100 to obtain an oil composition. This oil composition is added to 15-day-old experimental broilers at 5% by weight of the basic feed. The feed and oil are premixed. The feed is given in a fixed amount every day. The broilers can eat and drink freely. Before feeding, the leftover feed in the feed trough is removed and the weight is recorded.

[0048] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition obtained in this example is 2.67, the calcium absorption rate is 73.64%, the phosphorus absorption rate is 64.07%, and the lipid absorption rate is 75.45%.

[0049] Example 6

[0050] Except for the following technical features, this embodiment is the same as that in embodiment 1: Step (2) mixes sterol laurate with enzymatically modified palm oil (58% saturated fatty acid content at sn-2 position and 55% UPU content) at a mass ratio of 2:100 to obtain an oil composition. Add the oil composition to the animal chickens at 5% of the weight of the basic feed. The feed and oil are premixed. Feed is given in a fixed amount every day. The chickens can eat and drink freely. Before feeding, the leftover feed in the feed trough is removed and the weight is recorded.

[0051] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the oil composition in this example is 2.61, the calcium absorption rate is 71.95%, the phosphorus absorption rate is 73.15%, and the lipid absorption rate is 81.17%.

[0052] Comparative Example 1

[0053] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed was raw palm oil (sn-2 position saturated fatty acid content of 12% and UPU content of 23%), without transesterification. Raw palm oil was added to 15-day-old experimental broilers at 5% by weight of the basal feed. The feed and oil were premixed, and the feed was given in fixed quantities every day. The broilers had free access to food and water, and the remaining feed in the trough was removed and the weight was recorded before feeding.

[0054] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the raw material palm oil obtained in this example is 2.78, the calcium absorption rate is 52.95%, the phosphorus absorption rate is 53.78%, and the lipid absorption rate is 71.18%.

[0055] Comparative Example 2

[0056] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed was enzyme-modified palm oil (47% saturated fatty acid content at the sn-2 position and 43% UPU content). The enzyme-modified palm oil was added to the basal feed at 5% by weight and fed to 15-day-old experimental broilers. The feed and oil were premixed, and the feed was given in fixed quantities every day. The broilers had free access to food and water, and the remaining feed in the trough was removed and the weight was recorded before feeding.

[0057] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the enzymatically modified palm oil in this example was 2.74, the calcium absorption rate was 64.27%, the phosphorus absorption rate was 56.38%, and the lipid absorption rate was 78.26%.

[0058] Comparative Example 3

[0059] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed was enzyme-modified palm oil (sn-2 position saturated fatty acid content of 34% and UPU content of 28%). The enzyme-modified palm oil was added to the basal feed at 5% by weight and fed to 15-day-old experimental broilers. The feed and oil were premixed, and the feed was given in fixed quantities every day. The broilers had free access to food and water, and the remaining feed in the trough was removed and the weight was recorded before feeding.

[0060] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the enzymatically modified palm oil in this example was 2.79, the calcium absorption rate was 58.62%, the phosphorus absorption rate was 57.57%, and the lipid absorption rate was 74.94%.

[0061] Comparative Example 4

[0062] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed was enzyme-modified palm oil (58% saturated fatty acid content at the sn-2 position and 55% UPU content). The enzyme-modified palm oil was added to the basal feed at 5% by weight and fed to 15-day-old experimental broilers. The feed and oil were premixed, and the feed was given in fixed quantities every day. The broilers had free access to food and water, and the remaining feed in the trough was removed and the weight was recorded before feeding.

[0063] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio of the enzymatically modified palm oil obtained in this example is 2.73, the calcium absorption rate is 62.37%, the phosphorus absorption rate is 57.83%, and the lipid absorption rate is 81.87%.

[0064] Comparative Example 5

[0065] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed consists of esterified sterol lauric acid and raw material palm oil (sn-2 position saturated fatty acid content of 12%, UPU content of 23%) mixed at a mass ratio of 1:100. This mixed oil is added to 15-day-old experimental broilers at 5% by weight of the basal feed. The feed and oil are premixed, and a fixed amount is given daily. The broilers have free access to food and water, and any remaining feed in the trough is removed and the weight is recorded before feeding.

[0066] Based on the method described in Example 1 and the calculations performed, the meat ratio of the mixed oil feed obtained in this example is 2.68, the calcium absorption rate is 71.29%, the phosphorus absorption rate is 64.52%, and the lipid absorption rate is 77.54%.

[0067] Comparative Example 6

[0068] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed consists of esterified sterol lauric acid and raw palm oil (sn-2 position saturated fatty acid content of 12%, UPU content of 23%) at a mass ratio of 2:100. This mixed oil is added to 15-day-old experimental broilers at 5% by weight of the basal feed. The feed and oil are premixed, and a fixed amount is given daily. The broilers have free access to food and water, and any remaining feed in the trough is removed and the weight is recorded before feeding.

[0069] Based on the method described in Example 1 and the calculations performed, the meat ratio of the mixed oil feed obtained in this example is 2.66, the calcium absorption rate is 66.33%, the phosphorus absorption rate is 58.83%, and the lipid absorption rate is 74.87%.

[0070] Comparative Example 7

[0071] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed consists of esterified sterol lauric acid and raw palm oil (sn-2 position saturated fatty acid content of 12%, UPU content of 23%) at a mass ratio of 3:100. This mixed oil is added to 15-day-old experimental broilers at 5% by weight of the basal feed. The feed and oil are premixed, and a fixed amount is given daily. The broilers have free access to food and water, and any remaining feed in the trough is removed and the weight is recorded before feeding.

[0072] Based on the method described in Example 1 and the calculations performed, the meat ratio of the mixed oil feed obtained in this example is 2.71, the calcium absorption rate is 72.62%, the phosphorus absorption rate is 63.17%, and the lipid absorption rate is 76.35%.

[0073] Comparative Example 8

[0074] This comparative example is the same as Example 1 except for the following technical features: In step (2), the feed is esterified sterol lauric acid, which is added to the feed animal chickens at 1‰ of the weight of the basic feed.

[0075] Based on the method described in Example 1 and the calculations performed, the feed conversion ratio obtained in this example is 2.81, the calcium absorption rate is 68.62%, the phosphorus absorption rate is 57.28%, and the lipid absorption rate is 57.83%.

[0076] Table 1 shows the Sn-2 position saturated fatty acid and UPU content data of the initial oils in the examples and comparative examples.

[0077] Table 2 shows the feed conversion ratio (FCR) data of the products from the examples and comparative examples after 30 days of feeding.

[0078] Table 3 shows the calcium, phosphorus, and lipid absorption rates of the products from the examples and comparative examples after 30 days of feeding.

[0079] Table 4 shows the basic feed formulation data. The compound vitamins consist of 96 parts by weight of vitamin A, 98 parts by weight of vitamin B3, 12 parts by weight of vitamin B6, 1 part by weight of vitamin B12, 15 parts by weight of vitamin D3, 130 parts by weight of vitamin E, 20 parts by weight of vitamin K3, 16 parts by weight of thiamine, and 36 parts by weight of riboflavin. The minerals consist of 184 parts by weight of ferrous sulfate, 137 parts by weight of manganese sulfate, 33 parts by weight of basic cuprous oxide, 214 parts by weight of zinc sulfate, 2 parts by weight of sodium selenite, and 1 part by weight of calcium iodate.

[0080] Table 1

[0081]

[0082] Table 2

[0083]

[0084]

[0085] Table 3

[0086]

[0087]

[0088] Table 4

[0089]

[0090] Conclusion: When esterified sterol laurate and modified palm oil were used as dietary fat additives for poultry and broilers, the results after 30 days of continuous feeding showed that, compared with ordinary palm oil, modified palm oil and sterol laurate, and blends of sterol laurate and palm oil, the esterified sterol laurate and modified palm oil combination exhibited the highest absorption rate in animals, achieved a lower feed conversion ratio, and improved the absorption of calcium and phosphorus. Among all the examples, the best results were obtained in Example 1 with a 2% addition of sterol laurate.

[0091] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. An oil and fat composition, characterized in that... It includes sterol laurate and modified palm oil, wherein the mass ratio of sterol laurate to modified palm oil is 2:100; The modified palm oil is prepared by enzymatic transesterification of palm oil, wherein the content of saturated fatty acids at the sn-2 position of the modified palm oil is 47%; the palm oil used is commercially available 24℃ fractionated palm oil.

2. The oil composition according to claim 1, characterized in that: The enzyme used was the immobilized lipase Lipozyme TL IM.

3. The oil composition according to claim 1, characterized in that: The enzyme used was immobilized lipase Lipozyme TL IM, with an enzyme dosage of 0.5-2.5% of the palm oil mass. The transesterification reaction temperature was 60-80℃, and the reaction time was 15-60 min.

4. The oil composition according to claim 1, characterized in that: The sterol laurate is prepared by the following steps: sterol and lauric acid are catalytically esterified under vacuum and high temperature to obtain the sterol laurate product.

5. The oil composition according to claim 4, characterized in that: The vacuum condition is an absolute pressure of 3000-5000 Pa; The high-temperature conditions mentioned are 160-200℃.

6. The oil composition according to claim 5, characterized in that: The esterification reaction is a chemical esterification, wherein the catalyst used is concentrated sulfuric acid, the amount of catalyst added is 0.5-1% of the substrate mass, and the reaction time is 45-80 min; The mass ratio of the sterol to lauric acid is 0.5:1 to 1.2:

1.

7. A method for preparing an oil and fat composition according to any one of claims 1-6, characterized in that... Includes the following steps: The modified palm oil and sterol laurate are mixed thoroughly to obtain the oil composition.

8. The use of the oil composition according to any one of claims 1-6 as an oil additive in animal feed.

9. The use of the oil composition according to any one of claims 1-6 as an oil additive in broiler feed.

10. The application of the oil composition according to claim 8 as an oil additive in animal feed, characterized in that: The oil composition is premixed into the basal feed and then fed, with the oil composition accounting for 3-8% of the feed weight.

Citation Information

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