A sample loading device for sample pre-treatment and method of use thereof

Abstract

The application provides a sample loading device for sample pretreatment and a method for using the same. The sample loading device comprises a valve body and a valve core. The valve body is connected with a cleaning needle capable of cleaning a sample bottle. The cleaning needle is connected with a vertical cleaning channel in the valve body. A transverse cleaning channel is arranged in the valve body. A cleaning groove is arranged on the outer surface of the valve core and transversely offset from the central channel. An activation groove is arranged on the outer surface of the valve core. The front end of the activation groove is the same in cross section as the central channel and is offset from the central channel. The rear end of the activation groove is the same in cross section as the cleaning groove. An activation channel is arranged in the valve body. A solvent channel is arranged in the valve body in a vertical manner. The solvent channel is connected with the transverse cleaning channel and the activation channel. The application has the advantages of optimizing the flow path system and being capable of adapting to diversified sample loading requirements, especially the loading of dirty samples.

Description

TECHNICAL FIELD

[0001] The present application relates to a sample loading device for sample pretreatment, in particular to a sample loading device capable of meeting the requirements of a full-automatic solid phase extraction instrument in the process of sample loading, such as the placement of sample containers, the switching of sample loading on and off, the cleaning of sample containers, and the like, and even in the case of a sample containing dirty components, the sample loading device can still quickly place the sample containers and smoothly complete the sample loading and solid phase extraction processes. BACKGROUND

[0002] Currently, the loading methods of samples mainly include manual sample loading and full-automatic sample loading. Manual sample loading is to manually pour the sample when the sample needs to be loaded during the experiment according to the test requirements. This method has low automation degree, needs to occupy a large amount of human resources, has high labor intensity, and the uncertain factors in the pouring process can affect the determination of the experimental results. Full-automatic sample loading is to add valve elements in the sample loading pipeline, and to realize the loading of the sample by controlling the on and off of the valve. Compared with manual loading, the full-automatic sample loading has higher automation degree. However, when the sample is a dirty sample or contains a certain particle size, the valve elements are prone to leakage due to impurities, and even the valve and the pipeline are blocked to cause the failure of normal loading or the damage of the elements. Therefore, the above two sample loading methods cannot meet the automatic loading of dirty samples. SUMMARY

[0003] The present application aims to provide a sample loading device for sample pretreatment and a use method thereof, which can meet the automatic loading requirements of diversified samples, and in particular, can meet the requirements of quickly replacing the sample containers, controlling the smooth loading of the sample, and the automatic cleaning of the sample containers during the loading process of dirty samples.

[0004] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0005] A sample loading device for sample pretreatment, comprising a valve body and a valve core, wherein:

[0006] The upper part of the valve body can be sealingly connected with a sample bottle, and the lower part can be sealingly connected with a sample flow pipe. An upper sample channel is arranged between the upper part and the lower part. A valve hole is arranged in the middle part of the valve body in the transverse direction, and the valve hole intersects with the upper sample channel.

[0007] The valve core is made of corrosion-resistant material, and the front end of the valve core is inserted into the valve hole of the valve body in the transverse direction. A central channel is arranged at the intersection position of the valve core and the upper sample channel.

[0008] Its characterized in that: the valve body is connected with a cleaning needle which can clean the sample bottle upward, the cleaning needle is communicated with a vertical cleaning channel in the valve body, and a transverse cleaning channel is also arranged in the valve body, a cleaning groove is arranged on the outer surface of the valve core and is transversely staggered with the central channel, when the valve core rotates to the closed position, the central channel of the valve core rotates to the position which is not communicated with the sample channel, the cleaning groove is staggered with the vertical cleaning channel and the transverse cleaning channel, and when the valve core rotates to the open position, the central channel of the valve core rotates to the position which is coaxially communicated with the sample channel, the cleaning groove communicates the vertical cleaning channel and the transverse cleaning channel;

[0009] A activation groove is also arranged on the outer surface of the valve core, the front end of the activation groove is the same cross section with the central channel and is staggered with the central channel, the rear end of the activation groove is the same cross section with the cleaning groove, an activation channel is arranged in the valve body, when the valve core rotates to the closed position, the activation groove communicates the activation channel and the lower half of the sample channel, and when the valve core rotates to the open position, the activation groove is not communicated with the activation channel and the lower half of the sample channel;

[0010] A solvent channel is also arranged in the valve body vertically, the solvent channel is communicated with the transverse cleaning channel and the activation channel.

[0011] The sample loading device for sample pretreatment, wherein: a wave spring is sleeved on the valve core, the rear side of the wave spring abuts against a fixed screw ring, the fixed screw ring is threadedly connected with the valve body and is pre-tightened by a jack screw to press the wave spring, and the valve core is press-connected with the valve hole of the valve body by the elastic force of the wave spring.

[0012] The sample loading device for sample pretreatment, wherein: the rear end of the valve core is connected with a synchronous rotating connecting block, the rear end of the connecting block is connected with a motor, and the rotating movement of the valve core is controlled by the motor.

[0013] The sample loading device for sample pretreatment, wherein: a valve seat is further included, the valve seat has a horizontal base plate and a vertical mounting plate fixed to the base plate, the valve core is rotatably mounted on the vertical mounting plate, two positioning holes and a solvent flow channel are arranged on the base plate, and a solvent connector is tightly connected in the solvent flow channel;

[0014] The valve body is mounted on the base plate from top to bottom, two positioning columns and a sealing groove are arranged on the bottom surface of the valve body, the two positioning columns are inserted into the two positioning holes on the base plate of the valve seat, and the sealing groove is accurately butted with the solvent connector on the solvent flow channel to form a seal.

[0015] The lower end of the solvent channel passes through the solvent joint to communicate with the solvent flow channel.

[0016] A method for using a sample loading device for sample pretreatment, using the sample loading device for sample pretreatment, characterized in that:

[0017] In the sealed state, the valve core is in the closed state, the central channel of the valve core and the upper loading channel of the valve body are perpendicular and not connected to each other, the vertical cleaning channel and the horizontal cleaning channel on the valve body are separated by the valve core and cannot be connected, and the sample is sealed in the sample bottle.

[0018] The method for using the sample loading device for sample pretreatment, wherein: when the system requirement is activation, the valve core is in the closed state, and the activation solvent is introduced into the solvent channel, and since the horizontal cleaning channel is closed by the valve core, the activation solvent can only flow through the activation channel, at this time, one end of the activation groove of the valve core is connected to the activation channel, and the other end of the activation groove is connected to the lower half of the upper loading channel of the valve body, and the activation solution flows through the lower half of the upper loading channel to the sample flow tube and then to the extraction unit for activation.

[0019] The method for using the sample loading device for sample pretreatment, wherein: when the system requirement is to load the sample, the valve core is in the open state, the central channel of the valve core and the upper loading channel of the valve body are coaxial and connected, and the sample in the sample bottle can flow smoothly into the sample flow tube and then into the extraction unit; the vertical cleaning channel and the horizontal cleaning channel on the loading valve are connected through the cleaning groove on the valve core, at this time, nitrogen is introduced into the solvent channel of the valve body, and the nitrogen enters the sample bottle through the horizontal cleaning channel, the cleaning groove, the vertical cleaning channel, and the cleaning needle, which helps the sample to flow out smoothly.

[0020] The method for using the sample loading device for sample pretreatment, wherein: when the system requirement is cleaning, the valve core is in the open state, and the cleaning liquid is introduced into the solvent channel of the valve body, and the cleaning liquid flows through the horizontal cleaning channel, the cleaning groove, the vertical cleaning channel, and the cleaning needle to be injected into the sample bottle, and the cleaning liquid is sprayed on the inner wall of the sample bottle, and then falls back and flows out through the central channel of the valve core and the upper loading channel of the valve body.

[0021] The method for using the sample loading device for sample pretreatment, wherein: when the system requirement is bottle washing and elution, the valve core is in the open state, and the elution solvent is introduced into the solvent channel of the valve body, and the elution solvent flows through the horizontal cleaning channel, the cleaning groove, the vertical cleaning channel, and the cleaning needle to be injected into the sample bottle, and then flows out through the central channel of the valve core and the upper loading channel of the valve body.

[0022] The method for using the sample loading device of the sample pre-treatment, wherein: when the system requirement is not to wash the bottle, the valve core is in the closed state, the elution solvent is introduced into the solvent channel of the valve body, the elution solvent flows through the activation channel and the activation groove on the valve core, and finally flows out through the sample flow pipe.

[0023] The advantage of the present application is to optimize the flow path system, to adapt to diversified sample loading requirements, especially the sample loading of dirty samples, to realize the rapid installation of sample loading containers for various samples, and to control the smooth sample loading, cleaning of the sample loading container and various requirements. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is the center section view of the valve core when the sample valve is closed.

[0025] Figure 2 is the section view of the solvent channel when the sample valve is closed.

[0026] Figure 3 is the center section view of the valve core when the sample valve is opened.

[0027] Figure 4 is the section view of the solvent channel when the sample valve is opened.

[0028] Figure 5 is the half section view of the sample valve body when the sample valve is closed.

[0029] Figure 6 is the exploded view of the sample loading device.

[0030] BRIEF DESCRIPTION OF DRAWINGS: 1-valve body; 101-vertical cleaning channel; 102-horizontal cleaning channel; 103-activation channel; 104-solvent channel; 105-sealing groove; 106-sample loading channel; 107-gripping groove; 2-sample flow pipe; 3-valve core; 301-center channel; 302-cleaning groove; 303-activation groove; 4-wave spring; 5-fixing screw ring; 6-bottle sealing ring; 7-top screw; 8-cleaning needle; 9-positioning column; 10-sample bottle; 11-valve seat; 1102-positioning hole; 1103-solvent flow channel; 12-solvent connector; 13-solvent sealing ring; 14-connection block; 15-vertical mounting plate; 151-connection rod; 16-base plate. DETAILED DESCRIPTION

[0031] As shown in Figures 1-6 , the present application provides a sample loading device for sample pre-treatment, comprising a valve seat 11, a valve body 1 and a valve core 3, wherein:

[0032] The valve seat 11 is composed of a base plate 16 and a vertical mounting plate 15, and the bottom of the vertical mounting plate 15 is fixedly connected to one end of the base plate 16 through two connection rods 151.

[0033] The base plate 16 is provided with a U-shaped slot through which the sample flow pipe 2 passes from top to bottom, the sample flow pipe 2 being used to introduce sample or solvent into the extraction unit, and the base plate 16 is provided with two positioning holes 1102 and a solvent flow channel 1103, the solvent flow channel 1103 being tightly connected with the solvent joint 12;

[0034] The valve body 1 is installed on the base plate 16 from top to bottom and is ensured to be installed in place by gravity, the bottom surface of the valve body 1 is provided with two positioning columns 9 and a sealing groove 105, the two positioning columns 9 are inserted into the two positioning holes 1102 of the base plate 16, and the sealing groove 105 is accurately connected with the solvent joint 12 on the solvent flow channel 1103 to form a seal (a solvent sealing ring 13 is further arranged between the solvent joint 12 and the sealing groove 105); the upper part of the valve body 1 has a conical opening, the sample bottle 10 is connected with the valve body 1 through threads at the upper part of the conical opening, a bottle sealing ring 6 is further arranged between the sample bottle 10 and the valve body 1, the lower part of the valve body 1 is connected with the sample flow pipe 2 through threads, and the upper sample channel 106 is arranged in the middle of the valve body 1 and penetrates the valve body 1 from top to bottom; the two sides of the valve body 1 are provided with gripping grooves 107 for facilitating hand holding;

[0035] The valve core 3 is rotatably installed on the vertical installation plate 15 of the valve seat 11, the front end of the valve core 3 is conical and is inserted into the valve hole of the valve body 1 in the transverse direction, the cross-sectional position where the valve core 3 intersects with the upper sample channel 106 is provided with a central passage 301, and the rear end of the valve core 3 is connected with the connecting block 14 which can be used to control the rotation of the valve core 3;

[0036] A wave spring 4 is sleeved on the valve core 3, the rear side of the wave spring 4 abuts against a fixed screw ring 5, the fixed screw ring 5 is threadedly connected with the valve body 1 and is pre-tightened by the top wire 5 to press the wave spring 4, so that the conical part of the valve core 3 is tightly connected with the valve hole of the valve body 1 by the elastic force of the wave spring 4;

[0037] The front end of the connecting block 14 is inserted into the valve core 3, the cross section of the insertion position is non-circular, so that the connecting block 14 can drive the valve core 3 to rotate synchronously, and the rear end of the connecting block 14 is further connected with a motor (not shown in the figure), the motor is installed on the vertical installation plate 15 and is used to control the rotation angle (i.e. the opening and closing position) of the valve core 3. As shown in Figure 1 When the valve core 3 is in the closed position, the central passage 301 and the upper sample channel 106 are perpendicular to each other and are isolated, the sample bottle 10 and the sample flow pipe 2 are not connected, and when the valve core 3 is rotated by 90°, as shown in Figure 3As shown, the valve core 3 is in the open position, at which time the center channel 301 is coaxial with the sample channel 106, and the sample bottle 10 is in communication with the sample flow pipe 2; wherein the conical opening at the top of the valve body 1 (laterally offset from the sample channel 106) is further connected upwardly with a cleaning needle 8 for cleaning the sample bottle 10, the cleaning needle 8 being in communication with a vertical cleaning channel 101 in the valve body 1, and the valve body 1 is further provided with a horizontal cleaning channel 102, and the outer surface of the valve core 3 is provided with a cleaning groove 302 at a position laterally offset from the center channel 301, as shown in Figure 2 As shown, the cleaning groove 302 is offset from the vertical cleaning channel 101 and the horizontal cleaning channel 102, and when the valve core 3 is rotated to the open position, as shown in Figure 4 As shown, the cleaning groove 302 connects the vertical cleaning channel 101 and the horizontal cleaning channel 102;

[0038] A diagonally arranged activation groove 303 is further provided on the outer surface of the valve core 3, the front end of the activation groove 303 is the same cross section as the center channel 301 and is offset by 90 degrees from the center channel 301, and the rear end of the activation groove 303 is the same cross section as the cleaning groove 302, and a vertical activation channel 103 is provided in the valve body 1, which is parallel to and lower than the horizontal cleaning channel 102, and the outer ends of the horizontal cleaning channel 102 and the activation channel 103 are sealed by process hole plugs 108; as shown in Figure 2 As shown, the rear end of the activation groove 303 is in communication with the activation channel 103, at which time the front end of the activation groove 303 is in communication with the lower half of the sample channel 106, and when the valve core 3 is rotated to the open position, as shown in Figure 4 As shown, the activation groove 303 is offset from the activation channel 103, at which time the front end of the activation groove 303 is offset from and not in communication with the lower half of the sample channel 106;

[0039] A vertical solvent channel 104 is further provided in the valve body 1, the upper end of the solvent channel 104 is in communication with the horizontal cleaning channel 102 and the activation channel 103, and the lower end is in communication with the solvent flow channel 1103 after passing through the solvent joint 12.

[0040] The use process of the present application is as follows:

[0041] The valve core 3 is first in the state shown in Figure 1 and Figure 2 As shown in Figure 1 and Figure 2(These are different cross-sectional views of the same position on valve core 3). The central channel 301 of valve core 3 and the sample loading channel 106 of valve body 1 are perpendicular to each other and do not communicate. The vertical cleaning channel 101 and the horizontal cleaning channel 102 on valve body 1 are separated by valve core 3 and cannot communicate, thus sealing the sample in sample vial 10. This structure avoids the drawback of needing to add a separate one-way valve for throttling when installing the sample loading module, achieving a double sealing function with only valve core 3 and valve body 1.

[0042] When the system requires activation, the direction of valve core 3 is as follows: Figure 1 and Figure 2 As shown, activation solvent is introduced into the solvent flow channel 1103 of the valve seat 11. The activation solvent enters the solvent channel 104 of the valve body 1 through the solvent connector 12. Since the transverse cleaning channel 102 is closed by the valve core, the activation solvent can only flow through the activation channel 103. At this time, one end of the activation channel 103 and the activation tank 303 of the valve core 3 are connected (e.g., Figure 2 The other end of the activation tank 303 is connected to the lower half of the sample loading channel 106 of the valve body 1 (e.g., Figure 5 The activation solution flows through the sample loading channel 106 and finally through the sample flow tube 2 to the extraction unit (not shown in the figure) for activation.

[0043] When the system requires loading a sample, the valve core direction is as follows: Figure 3 and Figure 4 As shown ( Figure 3 and Figure 4 (Different cross-sectional views of the same position of valve core 3) The central channel 301 of valve core 3 and the sample loading channel 106 of valve body 1 are coaxial and connected, allowing the sample in sample vial 10 to flow smoothly into sample flow tube 2 and thus into extraction unit; the vertical cleaning channel 101 and the horizontal cleaning channel 102 on the sample loading valve 3 are connected through the cleaning groove 302 on valve core 3. At this time, nitrogen gas is introduced from the solvent flow channel 1103 of valve seat 11. The nitrogen gas enters the solvent channel 104 of valve body 1 through solvent connector 12, and then enters the sample vial 10 through the horizontal cleaning channel 102, cleaning groove 302, vertical cleaning channel 101 and cleaning needle 8, which helps the sample to flow out smoothly.

[0044] When the system requirement is cleaning, the valve core direction is as follows: Figure 3 and Figure 4 As shown, cleaning fluid is introduced into the solvent flow channel 1103 of the valve seat 11. The cleaning fluid enters the solvent channel 104 of the valve body 1 through the solvent connector 12, flows through the horizontal cleaning channel 102, the cleaning tank 302, the vertical cleaning channel 101 and the cleaning needle 8 and is injected into the sample bottle 10. The cleaning fluid is sprayed on the inner wall of the sample bottle 10, and after rinsing the residual sample, it falls back and flows through the central channel 301 of the valve core 3 and the sample loading channel 106 of the valve body 1, and finally flows out through the sample flow tube 2, completing the process of cleaning the sample bottle.

[0045] When the system requirement is over-bottle elution, the valve core direction is as shown in Figure 3 and Figure 4 The elution solvent is introduced from the solvent flow channel 1103 of the valve seat 11, and the elution solvent enters the solvent channel 104 of the valve body 1 through the solvent joint 12, flows through the transverse washing channel 102, the washing groove 302, the vertical washing channel 101 and the washing needle 8 into the sample bottle 10, and finally flows out through the sample flow pipe 2, completing the over-bottle elution process.

[0046] When the system requirement is not over-bottle elution, the valve core direction is as shown in Figure 1 and Figure 2 The elution solvent is introduced from the solvent flow channel 1103 of the valve seat 11, and the elution solvent enters the solvent channel 104 of the valve body 1 through the solvent joint 12, flows through the activation channel 103, the activation groove 303 on the valve core 3, and flows into the lower half of the sample loading channel 106 of the valve body 1, and finally flows out through the sample flow pipe 2, completing the not over-bottle elution process.

[0047] In the above embodiments, the solvent flow channel 1103 and the solvent joint 12 can also not be provided, and various solvents or gases can be directly supplied to the solvent channel 14.

[0048] The above process completes the whole process of activation, sample loading, washing and elution in the solid phase extraction process.

[0049] The above description is only illustrative and not limiting, and those skilled in the art understand that many modifications, changes or equivalents can be made without departing from the spirit and scope of the claims, and all will fall within the protection scope of the present application.

Claims

1. A sample loading device for sample pretreatment, comprising a valve body and a valve core, wherein: The upper part of the valve body can be sealed to the sample vial, and the lower part can be sealed to the sample flow tube. A through sample loading channel is provided between the upper and lower parts. A valve hole is provided in the middle of the valve body along the lateral direction, and the valve hole intersects with the sample loading channel. The front end of the valve core is inserted laterally into the valve hole of the valve body, and a through central channel is provided at the cross-section where the valve core intersects with the sample loading channel; The valve body is characterized by having an upwardly connected cleaning needle for cleaning the sample vial. The cleaning needle is connected to a vertical cleaning channel within the valve body, which also contains a horizontal cleaning channel. A cleaning groove is located on the outer surface of the valve core at a position horizontally offset from the central channel. When the valve core is rotated to the closed position, the central channel of the valve core rotates to a position where it is not connected to the sample loading channel, and the cleaning groove is offset from both the vertical and horizontal cleaning channels. When the valve core is rotated to the open position, the central channel of the valve core rotates to a position where it is coaxially connected to the sample loading channel, and the cleaning groove connects the vertical and horizontal cleaning channels. An activation groove is provided on the outer surface of the valve core. The front end of the activation groove has the same cross-section as the central channel and is offset from the central channel. The rear end of the activation groove has the same cross-section as the cleaning groove. An activation channel is provided in the valve body. When the valve core is rotated to the closed position, the activation groove connects the activation channel and the lower half of the sample loading channel. When the valve core is rotated to the open position, the activation groove is not connected to the activation channel and the lower half of the sample loading channel. The valve body is also provided with a vertically arranged solvent channel, which is connected to the horizontal cleaning channel and the activation channel.

2. The sample loading device for sample pretreatment according to claim 1, characterized in that: A wave spring is fitted onto the valve core, and a fixing screw ring abuts against the rear side of the wave spring. The fixing screw ring is threaded to the valve body and is pre-tightened by a set screw to press the wave spring. The valve core is pressed and connected to the valve hole of the valve body by the elastic force of the wave spring.

3. The sample loading device for sample pretreatment according to claim 1, characterized in that: The rear end of the valve core is connected to a synchronously rotating connecting block, and the rear end of the connecting block is connected to a motor, which controls the rotation of the valve core.

4. The sample loading device for sample pretreatment according to claim 1, characterized in that: It also includes a valve seat, which has a horizontally placed base plate and a vertical mounting plate fixed to the base plate. The valve core is rotatably mounted on the vertical mounting plate. The base plate is provided with two positioning holes and a solvent flow channel. A solvent connector is tightly connected inside the solvent flow channel. The valve body is mounted on the base plate from top to bottom. Two positioning posts and a sealing groove are provided on the bottom surface of the valve body. The two positioning posts are inserted into the two positioning holes on the base of the valve seat, which limits the sealing groove to accurately align with the solvent connector on the solvent flow channel to form a seal. The lower end of the solvent channel passes through the solvent connector and is connected to the solvent flow channel.

5. A method of using a sample loading device for sample pretreatment, wherein the sample loading device for sample pretreatment as described in claim 1 is used, characterized in that: In the sealed state, the valve core is closed, the central channel of the valve core and the sample loading channel of the valve body are perpendicular and not connected to each other, the vertical cleaning channel and the horizontal cleaning channel on the valve body are separated by the valve core and cannot be connected, and the sample is sealed in the sample bottle.

6. The method of using the sample loading device for sample pretreatment according to claim 5, characterized in that: When the system requires activation, the valve core is in the closed state. Activation solvent is introduced into the solvent channel. Since the transverse cleaning channel is closed by the valve core, the activation solvent can only flow through the activation channel. At this time, one end of the activation channel and the activation tank of the valve core are connected, while the other end of the activation tank is connected to the lower half of the sample loading channel of the valve body. The activation solution flows through the lower half of the sample loading channel and the sample flow tube to the extraction unit for activation.

7. The method of using the sample loading device for sample pretreatment according to claim 5, characterized in that: When the system requires sample loading, the valve core is in the open state. The central channel of the valve core and the sample loading channel of the valve body are coaxial and connected, allowing the sample in the sample vial to flow smoothly into the sample flow tube and enter the extraction unit. The vertical and horizontal cleaning channels on the sample loading valve are connected through the cleaning tank on the valve core. At this time, nitrogen gas is introduced into the solvent channel of the valve body. The nitrogen gas enters the sample vial through the horizontal cleaning channel, the cleaning tank, the vertical cleaning channel, and the cleaning needle, which helps the sample to flow out smoothly.

8. The method of using the sample loading device for sample pretreatment according to claim 5, characterized in that: When the system requires cleaning, the valve core is in the open state, and the cleaning solution is introduced into the solvent channel of the valve body. The cleaning solution flows through the horizontal cleaning channel, the cleaning tank, the vertical cleaning channel, and the cleaning needle into the sample bottle. The cleaning solution is sprayed on the inner wall of the sample bottle, rinsing the residual sample, and then falls back through the central channel of the valve core and the sample loading channel of the valve body, and finally flows out through the sample flow tube.

9. The method of using the sample loading device for sample pretreatment according to claim 5, characterized in that: When the system requires vial elution, the valve core is in the open state, and the elution solvent is introduced into the solvent channel of the valve body. The elution solvent flows through the horizontal cleaning channel, the cleaning tank, the vertical cleaning channel and the cleaning needle into the sample vial, and then flows out through the central channel of the valve core and the sample loading channel of the valve body, and finally flows out through the sample flow tube.

10. The method of using the sample loading device for sample pretreatment according to claim 5, characterized in that: When the system requires non-bottle elution, the valve core is in the closed state, and the elution solvent is introduced into the solvent channel of the valve body. The elution solvent flows through the activation channel and the activation tank on the valve core into the lower half of the sample loading channel of the valve body, and finally flows out through the sample flow tube.

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