A method for shortening the production cycle of banana secondary tissue culture seedlings

Abstract

The application belongs to the technical field of banana tissue culture and specifically relates to a method for shortening the production cycle of banana secondary tissue culture seedlings. The application aims to solve the problem of long production cycle of banana secondary tissue culture seedlings. The technical scheme of the application is a method for shortening the production cycle of banana secondary tissue culture seedlings, which comprises the following steps: spraying a plant growth regulator on banana secondary tissue culture seedlings; the plant growth regulator is paclobutrazol and / or mepiquat chloride. The application provides a method for shortening the production cycle of banana secondary tissue culture seedlings. The treatment of banana secondary tissue culture seedlings with a plant growth regulator having a growth inhibiting function can make the secondary tissue culture seedlings have shorter stems, thicker stems, smaller leaves, thicker leaves, and a significantly increased chlorophyll SPAD value, so that the production cycle is shortened, the seedlings can be transplanted to a nursery 10-20 days earlier, the production efficiency of the nursery is improved, the economic benefit is improved, and the survival rate, growth period and yield of the banana seedlings after field planting are not affected.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of banana tissue culture, and particularly relates to a method for shortening the production cycle of banana secondary tissue culture seedlings. BACKGROUND

[0002] Bananas (Musa spp) belong to Musaceae and Musa, are important food crops and economic crops in the world, are staple food for nearly 600 million people in the world, and are the largest fruit in the world in terms of fresh fruit trade. China is a big country in the world in terms of banana production and consumption, and the harvested area and yield rank the third and the third in the world in 2020, and the consumption ranks the second in the world. At present, the cultivated banana species in China mainly include 'Xiangyabaixia' (AAA), 'Fenjiaoxia' (ABB), 'Longyabaixia' (AAB) and 'Gongjiaoxia' (AA) four categories. Among them, the planting area of 'Xiangyabaixia' and 'Fenjiaoxia' accounts for 97%. In 2020, the banana planting area in China was 4.89 million mu, and the number of banana secondary tissue culture seedlings required was 586.8 million.

[0003] In the mid and late 1980s in China, banana tissue culture seedlings were successfully developed, which led to the vigorous development of industrialized seedling production in banana production areas in the south. Banana tissue culture seedlings are accepted by banana farmers and planters due to their fast growth, detoxification, high yield, and convenient management. However, due to the influence of banana wilt, high-quality banana secondary tissue culture seedlings need to be produced in soilless substrates and supporting isolation facilities, and the labor cost and agricultural material cost are increasing year by year, which continuously increases the cost of seedling production enterprises, but the sales price of banana secondary tissue culture seedlings has been stable at 1.5-2.5 yuan per plant, which greatly discourages the enthusiasm of enterprises, and thus they give up producing high-quality seedlings, which is very unfavorable to the sustainable development of the banana industry. Banana secondary tissue culture seedlings need 50 days in summer and autumn and 80 days in spring and winter to reach the standard for leaving the nursery, so it is very important to shorten the production cycle of banana secondary tissue culture seedlings and improve the production efficiency of the nursery. At present, there are few technical researches on shortening the growth cycle of banana seedlings, and only methods such as using appropriate concentration of potassium nitrate and alginic acid as rooting agents to irrigate banana secondary tissue culture seedlings exist, but they are all cumbersome, labor-intensive, and not suitable for large-scale use. Therefore, it is necessary to develop a high-efficiency, easy-to-operate, low-cost technology for shortening the production cycle of banana secondary tissue culture seedlings and improving the production efficiency of the nursery.

[0004] Plant growth retardants are a class of plant growth regulators that increase plant stem thickness and shorten internodes. They affect plant growth characteristics by affecting the synthesis and transport of endogenous hormones, but do not affect the development process of plants. Common plant growth retardants include paclobutrazol and mepiquat chloride. In recent years, the application of plant growth retardants in seedling cultivation has been expanding, such as controlling seedling overgrowth, cultivating strong seedlings and preventing seedling death in rice, cucumber, bitter gourd, tomato, tobacco, sunflower and kale. SUMMARY

[0005] The technical problem solved by the present application is that the production cycle of banana secondary tissue culture seedlings is long.

[0006] The technical solution of the present application is a method for shortening the production cycle of banana secondary tissue culture seedlings, comprising the following steps: spraying plant growth regulators on banana secondary tissue culture seedlings; the plant growth regulator is a mixture of paclobutrazol and / or mepiquat chloride.

[0007] The concentration of the plant growth regulator is 10-100 mg / L.

[0008] Preferably, the concentration of the plant growth regulator is 30-80 mg / L.

[0009] Further, the plant growth regulator is paclobutrazol and mepiquat chloride, and the mass ratio of the two is 1:1.

[0010] The plant growth regulator is sprayed once or twice.

[0011] Specifically, the time interval for spraying twice is 1-2 leaves newly emerged from the secondary tissue culture seedlings.

[0012] The first spraying is performed at the 1-3 leaf stage, and the second spraying is performed at the 2-5 leaf stage.

[0013] Preferably, for Musa acuminata tissue culture seedlings, the first spraying is performed at the 1-2 leaf stage, and the second spraying is performed at the 2-4 leaf stage.

[0014] Further, the spraying is a leaf surface spraying treatment.

[0015] The amount of spraying is to cover the leaf surface with a layer of atomized particles.

[0016] Preferably, the amount of spraying is 15-25 mL / plant.

[0017] Specifically, the banana is a powder banana or a Musa acuminata.

[0018] Preferably, for powder banana tissue culture seedlings, the first spraying is performed at the 1-3 leaf stage, and the second spraying is performed at the 2-5 leaf stage.

[0019] Specifically, the plant growth regulator concentration used for Musa acuminata secondary tissue culture seedlings is 30-70 mg / L.

[0020] In particular, the plant growth regulator concentration used for powder banana secondary tissue culture seedlings is 40-80 mg / L.

[0021] The application provides a method for shortening the production period of banana secondary tissue culture seedlings, and the method comprises the following steps: sequentially performing first plant growth regulator treatment and second plant growth regulator treatment on the banana secondary tissue culture seedlings by using a plant growth regulator with a growth inhibition function, so that the stem height of the secondary tissue culture seedlings is shortened, the false stem is thickened, the leaf length and leaf width are reduced, the leaf thickness is increased, the SPAD value of chlorophyll is significantly increased, and the total number of leaves is increased; the production period is shortened, the seedlings can be taken out of the nursery 10-20 days in advance, the seedling efficiency is improved, the seedling cost is reduced, the economic benefit is improved, and the survival rate, growth period and yield of the banana seedlings after field planting are not affected. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 , the photos of the secondary tissue culture seedlings of the banana in 2022 when the seedlings were taken out of the nursery; in the figure, the concentrations from top to bottom represent 10 mg / L, 30 mg / L, 50 mg / L, 70 mg / L and 90 mg / L.

[0023] Figure 2 , the photos of the secondary tissue culture seedlings of the banana in 2022 when the seedlings were taken out of the nursery; in the figure, the concentrations from top to bottom represent 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L and 100 mg / L. DETAILED DESCRIPTION

[0024] In order to solve the problem of shortening the production period of banana secondary tissue culture seedlings, the inventors consider applying plant growth regulators used on other crops to the cultivation of banana tissue culture seedlings. First of all, multiple effects of azoles, methyl and chlormequat were selected for preliminary tests, and the results showed that multiple effects of azoles and methyl increased the total number of leaves of banana secondary tissue culture seedlings and significantly reduced the stem height of seedlings. Further, the applicant also optimized the dosage and application method of multiple effects of azoles and methyl. The results showed that both of them can play a role when used alone or in combination; especially when used in combination, the effect is better. In terms of dosage, concentrations above 20 mg / L can effectively shorten the production period of tissue culture seedlings; considering the production cost and plant development, the best ratio of multiple effects of azoles and methyl is 1:1, and the best concentration is 30-80 mg / L.

[0025] The plant growth regulator used in the following examples is purchased from Sichuan Guoguang Agricultural Chemical Co., Ltd., and the concentration of multiple effects of azoles is 15%; the concentration of methyl is 98%.

[0026] Example 1: Screening of plant growth regulators

[0027] In 2021, 450 banana secondary tissue culture seedlings with consistent growth and 5 leaves per heart were randomly selected as the experimental group, and 50 seedlings were used as the control group (only water was sprayed), the banana variety was “Guijiao No. 1”, and the experiment was repeated 3 times.

[0028] The experiment has 4 different treatments (see Table 1). When the banana secondary tissue culture seedlings grow to 5 leaves and 1 heart, the first treatment is carried out. According to the concentration in Table 1, a suitable amount of plant growth regulators such as paclobutrazol is dissolved in 20-40 mL of water, and the dose of 20-40 mL / plant is applied along the pseudostem of the banana seedlings. After 30 days, the same concentration of plant growth regulators is used for the second treatment of the banana seedlings. The control group is only irrigated with water in the same way as the plant growth regulator treatment group.

[0029] Table 1 Test design for irrigating banana secondary tissue culture seedlings with growth retardants

[0030]

[0031] After irrigating with plant growth regulators twice for 1 month, the stem height and total leaf number of the banana seedlings are measured. The stem height is the vertical distance from the substrate surface to the cross point of the petiole of the two newly emerged leaves.

[0032] Table 2 Effect determination results of irrigating banana secondary tissue culture seedlings with growth retardants

[0033] Treatment Control Paclobutrazol Mepiquat Chloride Chlormequat Chloride Total Leaf Number (sheets) 12.7 15.3* 14.7* 12.0 Stem Height (cm) 21.8 14.0* 16.9* 21.7

[0034] Note: * indicates a significant correlation at the 0.05 level.

[0035] As can be seen from Table 2, the total leaf number of the banana secondary tissue culture seedlings is significantly increased by 2.6 and 2.0 respectively by the two-time irrigation of 50 mg / L paclobutrazol and mepiquat chloride compared with the control. The stem height of the seedlings is significantly reduced by 7.8 cm and 4.9 cm respectively by the two-time irrigation of 50 mg / L paclobutrazol and mepiquat chloride compared with the control. However, the total leaf number and stem height of the banana seedlings treated with the secondary irrigation of 50 mg / L ethephon are not significantly different from the control.

[0036] Example 2 Cultivation of tissue culture seedlings by the method of the present application

[0037] In 2022, 1800 banana secondary tissue culture seedlings with consistent growth were randomly selected as the experimental group, and 60 seedlings were used as the control group (only sprayed with water) to carry out the banana secondary tissue culture seedling spraying plant growth regulator test. The banana variety was "Guijiao No. 1". Through the test, three plant growth regulators were set up, including paclobutrazol, mepiquat chloride, and 0.5 mepiquat chloride + 0.5 paclobutrazol (i.e. the total amount of the drug is the same as using it alone, and the mass of the two is half each). Five treatment concentrations (the concentration is the final concentration of plant growth regulators in the spraying solution) are 10 mg / L, 30 mg / L, 50 mg / L, 70 mg / L, and 90 mg / L. When the experimental group banana secondary tissue culture seedlings grow to 1 leaf with 1 heart, the first treatment is carried out, and paclobutrazol, mepiquat chloride, and 0.5 mepiquat chloride + 0.5 paclobutrazol are sprayed on the surface of each banana seedling leaf, covering the leaf surface with a layer of atomized particles, 20 mL per plant. After 7 days, the experimental group banana secondary tissue culture seedlings grow to 2 leaves with 1 heart, and the same concentration of plant growth regulators is used for the second treatment of banana seedlings; a total of 30 treatments, 20 banana secondary seedlings per treatment, 3 times repeated. The control group is only sprayed with water, and the treatment method is the same as the experimental group. The specific spraying method of plant growth regulators is shown in Table 3 below.

[0038] Table 3 Treatment design of banana secondary tissue culture seedling spraying growth retardant test

[0039]

[0040]

[0041]

[0042] Note: According to the "Banana Seedling Breeding Technical Regulations" (NY / T 3200-2018), the secondary tissue culture seedlings of fragrant banana or powder banana can be sold only if they reach 7 leaf age or above. In the previous experiment, it was found that spraying at 5 leaf 1 heart and 8 leaf 1 heart was too late, and the plant type was poor when it was sold, with a larger leaf age of 12 leaf age or above, which was quite different from the conventional seedlings sold, and was not conducive to the large-scale production of seedlings. Therefore, in the follow-up test, the use time will be advanced, so that the normal 7-8 leaf age seedlings can be sold, with good plant type, which is conducive to large-scale production.

[0043] After 40 days of spraying twice plant growth regulators, the following agronomic traits of banana seedlings treated with plant growth regulators once and twice were measured before the seedlings were sold:

[0044] Stem height: the vertical distance from the intersection point of the petiole of the latest two leaves to the substrate surface;

[0045] Stem diameter: the diameter at the middle of the stem was measured with a vernier caliper;

[0046] Leaf length: the second unfolded leaf was measured from the base of the leaf to the tip with a tape measure;

[0047] Leaf width: the widest part of the second expanded leaf blade was measured with a tape measure, perpendicular to the leaf length;

[0048] Leaf thickness: the thickness of the middle part of the second expanded leaf was measured with a vernier caliper;

[0049] Chlorophyll content: the chlorophyll content of the middle part of the second expanded leaf was measured with a TYS-B type chlorophyll meter.

[0050] As shown in Table 4 and Figure 1 It can be seen that all treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol reduced the stem height, leaf length and leaf width of the banana secondary tissue culture seedlings to varying degrees. And most of the treatments also increased the total leaf number, leaf thickness and chlorophyll content of the seedlings. Among them, compared with the control, except for the treatment of paclobutrazol once sprayed at 10 mg / L, mepiquat chloride twice sprayed at 10 mg / L, 30 mg / L, 70 mg / L, 90 mg / L, and mepiquat chloride + paclobutrazol once sprayed at 10 mg / L, the rest of the treatments increased the total leaf number of the banana secondary tissue culture seedlings by 0.1-2.4 leaves. The stem height of all 30 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol was lower than that of the control, with a decrease of 12.4%-78.4%. The stem diameter of the treatments of paclobutrazol once sprayed at 90 mg / L and twice sprayed at 30 mg / L, 50 mg / L, 70 mg / L, 90 mg / L, and 0.5 mepiquat chloride + 0.5 paclobutrazol twice sprayed at 70 mg / L, 90 mg / L was increased compared with the control, with an increase of 0.3%-53.4%. The leaf length and leaf width of all 30 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol were shorter and narrower than those of the control, with a decrease of 1.4%-64.0% and 5.2%-46.6%, respectively. Except for the treatments of mepiquat chloride twice sprayed at 10 mg / L, 30 mg / L, 70 mg / L, 90 mg / L, the rest of the treatments increased the chlorophyll SPAD value of the banana secondary tissue culture seedlings compared with the control, with an increase of 0.4%-32.4%.

[0051] Table 4 Effect of spraying growth retardants on the agronomic traits of banana secondary tissue culture seedlings

[0052]

[0053]

[0054] Note: * indicates significant correlation at the 0.05 level.

[0055] In order to further understand the effect of spraying growth retardants on the seedling raising period of banana secondary tissue culture seedlings, the seedling raising start time and exit time of the banana seedlings treated with plant growth regulators once and twice were counted.

[0056] Date of starting seedling: the date of starting to transplant the first group of tissue culture seedlings.

[0057] Date of leaving the nursery: the date when the number of the second group of tissue culture seedlings reaching 7-leaf stage accounts for 50%.

[0058] Table 5 Effects of spraying growth retardants on the growth cycle of the second group of banana tissue culture seedlings

[0059]

[0060]

[0061] Note: * indicates significant correlation at the 0.05 level.

[0062] As can be seen from Table 5 above, all treatments of PP333, mepiquat chloride and 0.5 mepiquat chloride + 0.5 PP333, except for the treatment of PP333 once sprayed at 10 mg / L, mepiquat chloride twice sprayed at 10 mg / L, 30 mg / L, 70 mg / L, 90 mg / L, and mepiquat chloride + PP333 once sprayed at 10 mg / L, shortened the growth cycle of the second group of banana tissue culture seedlings by 1-14 days compared with the control.

[0063] In 2022, the second group of banana tissue culture seedlings treated with PP333, mepiquat chloride at 10 mg / L, 30 mg / L, 70 mg / L, 90 mg / L will be planted in the field, and the effects in the later stage will be observed. Single row double row planting. 10 plants per treatment, 3 times, plant spacing 2.3 m x 1.75 m, and no other growth retardants will be used in the production process. In 2023, when the banana enters the harvest period, the following biological characteristics will be investigated.

[0064] Date of budding: the date when the number of the treated banana plants reaching 50% of the budding stage;

[0065] Date of harvest: the date when the number of the treated banana plants reaching 50% of the fruiting stage with 75-85% fullness.

[0066] Table 6 Effects of spraying growth retardants on the biological characteristics of "Guijiao No. 1"

[0067] Treatment Planting Time 50% Bud Breaking Time Harvesting Time Growth Period / d Control 2022.5.31 2023.1.29 2023.5.21 355 Two Sprays of 10 mg / L Paclobutrazol 2022.5.21 2023.1.18 2023.5.17 361 Two Sprays of 30 mg / L Paclobutrazol 2022.5.21 2023.1.16 2023.5.15 357 Two Sprays of 50 mg / L Paclobutrazol 2022.5.21 2023.1.20 2023.5.13 359 Two Sprays of 70 mg / L Paclobutrazol 2022.5.20 2023.1.18 2023.5.5 350 Two Sprays of 90 mg / L Paclobutrazol 2022.5.16 2023.1.15 2023.5.5 354 Two Sprays of 10 mg / L Mepiquat Chloride 2022.5.31 2023.1.26 2023.5.21 355 Two Sprays of 30 mg / L Mepiquat Chloride 2022.6.1 2023.2.1 2023.5.25 358 Two Sprays of 50 mg / L Mepiquat Chloride 2022.6.1 2023.2.5 2023.5.27 360 Two Sprays of 70 mg / L Mepiquat Chloride 2022.6.1 2023.2.3 2023.5.19 352 Two Sprays of 90 mg / L Mepiquat Chloride 2022.5.28 2023.1.22 2023.5.14 351

[0068] As can be seen from Table 6, spraying growth retardants during seedling does not affect the survival rate, yield or maturity period of the banana after planting. The yield of the second group of tissue culture seedlings treated with PP333 and mepiquat chloride twice has no significant difference with the control. The growth cycle of the banana seedlings treated with PP333 twice is 1-6 days longer than the control; the growth cycle of the banana seedlings treated with mepiquat chloride twice is 4-5 days longer than the control, with no significant difference.

[0069] Table 7 Effects of spraying growth retardants on yield of "Guijiao No. 1" As can be seen from Table 7, the single bunch weight of banana seedlings treated by spraying paclobutrazol and mepiquat twice was not significantly different from the control when the secondary tissue culture seedlings were bred.

[0070] Example 3 Breeding of tissue culture seedlings by the method of the application

[0071] In 2022, 900 secondary tissue culture seedlings of powdery banana with consistent growth were randomly selected as the experimental group, and 50 were used as the control group (only sprayed with water). The powdery banana variety was "Jin Fen No. 1". Three plant growth regulators, paclobutrazol, mepiquat, and 0.5 paclobutrazol + 0.5 mepiquat, were set up through the experiment, and five treatment concentrations were used: 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L, and 100 mg / L. When the experimental group of secondary tissue culture seedlings of powdery banana reached the 2-leaf heart stage, the first treatment was carried out, and paclobutrazol, mepiquat, and 0.5 paclobutrazol + 0.5 mepiquat were sprayed on the leaves of each banana seedling, covering the leaf surface with a layer of atomized particles, 25 mL per plant. After an interval of 5 days, the experimental group of secondary tissue culture seedlings of powdery banana reached the 3-leaf heart stage, and the same concentration of plant growth regulators was used for the second treatment of the powdery banana seedlings; a total of 15 treatments, 20 secondary seedlings of powdery banana per treatment, and 3 repetitions. The control group was only sprayed with water, and the treatment method was the same as that of the experimental group. The specific spraying method of plant growth regulators is shown in Table 8 below.

[0072] Table 8 Treatment design for spraying plant growth regulators on secondary tissue culture seedlings of powdery banana

[0073]

[0074]

[0075]

[0076] One month after the completion of the spraying of plant growth regulators twice, the following agronomic traits of the secondary seedlings of powdery banana in all treatments were measured, and the seedling period was investigated:

[0077] Stem height: the vertical distance from the intersection point of the petioles of the two newly emerging leaves to the surface of the substrate;

[0078] Stem diameter: the diameter at the middle part of the stem was measured using a vernier caliper;

[0079] Leaf length: the length of the second unfolded leaf from the base to the tip was measured using a tape measure;

[0080] Leaf width: the widest part of the second unfolded leaf was measured using a tape measure, and the measurement was perpendicular to the leaf length;

[0081] Leaf thickness: the thickness of the middle part of the second unfolded leaf was measured using a vernier caliper;

[0082] Chlorophyll content: The chlorophyll content of the middle part of the second expanded leaf was determined by Top TYS-B chlorophyll meter.

[0083] Table 9 Effect of spraying growth retardant on secondary tissue culture seedlings of Musa acuminata

[0084]

[0085] Note: * indicates significant correlation at the 0.05 level.

[0086] As can be seen from Table 9, the use of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol increased the total leaf number and leaf thickness of the secondary tissue culture seedlings of Musa acuminata to varying degrees and reduced the seedling height. Most of the treatments also increased the chlorophyll content of the leaves of the secondary tissue culture seedlings of Musa acuminata and reduced the leaf length and width. Compared with the control, all 15 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol increased the total leaf number of the secondary tissue culture seedlings of Musa acuminata by 0.8-2.7 leaves. The stem height of all 15 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol was significantly lower than that of the control, with a decrease of 13.1%-92.3%. Except for mepiquat chloride at 20 mg / L, 40 mg / L, 60 mg / L, 80 mg / L and 0.5 mepiquat chloride + 0.5 paclobutrazol at 20 mg / L, the stem diameter of the other treatments was increased by 14.2%-143.1% compared with the control. Except for mepiquat chloride at 20 mg / L, the leaf length of the other treatments was shorter than that of the control, with a decrease of 3.2%-63.2%. Except for mepiquat chloride at 20 mg / L, 40 mg / L, the leaf width of the other treatments was smaller than that of the control, with a decrease of 2%-29.8%. The leaf thickness of all 15 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol was significantly increased compared with the control, with an increase of 12%-100%. Except for mepiquat chloride at 20 mg / L, 40 mg / L, 80 mg / L, 100 mg / L, the chlorophyll SPAD value of the leaves of the secondary tissue culture seedlings of Musa acuminata was increased by 2.1%-37.8% compared with the control.

[0087] In order to further understand the effect of spraying growth retardant on the seedling raising period of the secondary tissue culture seedlings of Musa acuminata, the seedling raising start time and the time of reaching 50% of the number of seedlings with 7 leaf ages of the secondary tissue culture seedlings treated with plant growth regulators once and twice were counted.

[0088] Seedling raising start time: the date of starting to transplant the primary tissue culture seedlings.

[0089] Time of reaching 50%: the date of reaching 50% of the number of seedlings with 7 leaf ages of the secondary tissue culture seedlings.

[0090] Table 10 Effect of spraying growth retardant on the seedling raising period of the secondary tissue culture seedlings of Musa acuminata

[0091] Treatment Seedling Raising Start Time Out of the Nursery Time Seedling Raising Days (days) Control 2022.9.5 2022.10.27 52 20 mg / L Paclobutrazol 2022.9.5 2022.10.14 39 40 mg / L Paclobutrazol 2022.9.5 2022.10.16 41 60 mg / L Paclobutrazol 2022.9.5 2022.10.14 39 80 mg / L Paclobutrazol 2022.9.5 2022.10.20 45 100 mg / L Paclobutrazol 2022.9.5 2022.10.18 43 20 mg / L Mepiquat Chloride 2022.9.5 2022.10.19 44 40 mg / L Mepiquat Chloride 2022.9.5 2022.10.22 47 60 mg / L Mepiquat Chloride 2022.9.5 2022.10.21 46 80 mg / L Mepiquat Chloride 2022.9.5 2022.10.23 48 100 mg / L Mepiquat Chloride 2022.9.5 2022.10.23 48 20 mg / L 0.5 Mepiquat Chloride + 0.5 Paclobutrazol 2022.9.5 2022.10.23 48 40 mg / L 0.5 Mepiquat Chloride + 0.5 Paclobutrazol 2022.9.5 2022.10.21 46 60 mg / L 0.5 Mepiquat Chloride + 0.5 Paclobutrazol 2022.9.5 2022.10.20 45 80 mg / L 0.5 Mepiquat Chloride + 0.5 Paclobutrazol 2022.9.5 2022.10.21 46 100 mg / L 0.5 Mepiquat Chloride + 0.5 Paclobutrazol 2022.9.5 2022.10.20 45

[0092] As can be seen from Table 10 above, all of the 15 treatments of paclobutrazol, mepiquat chloride and 0.5 mepiquat chloride + 0.5 paclobutrazol shortened the growing period of the secondary group tissue culture seedlings of the banana, by 4-13 days.

[0093] The foregoing description of specific exemplary embodiments of the application has been presented for the purposes of illustration and description. It is not intended to be exhaustive or to limit the application to the precise form disclosed, and various modifications and variations are possible in light of the above teachings. It is intended that the application encompass all such modifications and variations as fall within the scope of the claims and their equivalents. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application to thereby enable others skilled in the art to best utilize the application and various embodiments with various modifications as are suited to the particular use contemplated. It is intended that the scope of the application be defined by the claims and their equivalents.

Claims

1. A method for shortening the production cycle of secondary tissue culture plantlets of banana, characterized in that, The method comprises the following steps: spraying plant growth regulator on the secondary tissue culture seedlings of banana; the concentration of the plant growth regulator is 30 mg / L, 50 mg / L or 70 mg / L, and the plant growth regulator is a mixture of paclobutrazol and mepiquat chloride with a ratio of 1:1; the plant growth regulator is sprayed twice, and the interval between the two times of spraying is 1-2 leaves newly grown by the secondary tissue culture seedlings; the first time of spraying is at the stage of 1-3 leaves, and the second time of spraying is at the stage of 2-5 leaves; the spraying is a leaf surface spraying treatment; the spraying amount is to cover the leaf surface with a layer of atomized particles; and the banana is powder banana or fragrant banana.

2. The method of claim 1, wherein: The spraying amount is 15-25 mL / plant.

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