A water-light needle skin care composition

Abstract

The present application belongs to the technical field of skin care products, and particularly relates to a water-light needle skin care composition. A preservative composition is composed of p-hydroxyacetophenone and laminarin at a mass ratio of 1:3. When p-hydroxyacetophenone and laminarin are used at a mass ratio of 1:3, the growth of molds such as Aspergillus versicolor and Penicillium purpurescens and bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus can be inhibited, and the effect is synergistic. Compared with the use of p-hydroxyacetophenone alone, the inhibitory effect on various microorganisms can be improved, the resistance of the water-light needle skin care composition to external microorganisms can be improved, and the storage time of the water-light needle skin care composition can be prolonged. At the same time, the addition amount of the preservative component in the water-light needle skin care composition can be reduced, and the influence on human skin and health can be avoided.

Description

Technical Field

[0001] This invention belongs to the field of skin care technology, specifically relating to a skin care composition for hyaluronic acid injections. Background Technology

[0002] After the age of 25, most people begin to show signs of aging, with facial wrinkles being a significant characteristic. As people's living standards improve, their demands for appearance also increase, naturally leading to higher requirements for safety. Hyaluronic acid injections can make the skin hydrated and radiant. Patent CN201811530558.5 discloses a topical hyaluronic acid injection essence and its preparation method. This invention discloses a topical hyaluronic acid injection essence that can quickly penetrate the basal layer of the skin, providing long-lasting hydration, making the skin delicate, firm, and elastic, maintaining the integrity of the stratum corneum's moisture and fiber structure, improving the skin cell survival environment, and promoting skin tissue metabolism. It has the effects of moisturizing the skin, delaying aging, reducing wrinkles, and rejuvenating the skin. To prevent external microorganisms from affecting its shelf life, this hyaluronic acid injection essence adds styrene as a preservative. However, using a single preservative can easily lead to resistance from external microorganisms, resulting in a reduced preservative effect; although increasing the dosage of a single preservative can improve the preservative effect, it can also affect human skin and overall health.

[0003] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0004] The purpose of this invention is to provide a skin care composition for hyaluronic acid injections to solve the problems existing in the use of a single preservative in the prior art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] The first objective of this invention is to provide a preservative composition for use in skin care products, the preservative composition comprising p-hydroxyacetophenone and laminarin in a mass ratio of 1:3.

[0007] The second objective of this invention is to provide a skincare composition for hyaluronic acid injections, which, by weight percentage, is prepared from the following components: 0.6-1.8% aloe vera extract, 0.4-0.8% steviol glycosides, 0.02-0.06% amino acids, 0.8-1.6% hyaluronic acid, 4.2-8.6% moisturizer, 0.3-0.6% thickener, 0.05-0.75% solubilizer, 0.1-0.2% of the preservative composition, and the balance being sterile water.

[0008] More specifically, by weight percentage, it is prepared from the following ingredients: 1.2% aloe vera extract, 0.6% steviol glycosides, 0.04% amino acids, 1.2% hyaluronic acid, 6.2% humectant, 0.4% thickener, 0.15% solubilizer, 0.15% of the preservative composition of claim 1, and the balance being sterile water.

[0009] More specifically, the amino acid is composed of aspartic acid, phenylalanine, methionine, and taurine.

[0010] More specifically, the mass ratio of aspartic acid, phenylalanine, methionine and taurine is 4:1:2:2.

[0011] More specifically, the molecular weight of the hyaluronic acid is 200,000 to 600,000 Daltons.

[0012] More specifically, the moisturizer is glycerin.

[0013] More specifically, the thickener is 1,2-ethylene glycol.

[0014] More specifically, the co-solvent is Tween-80.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] This invention demonstrates that when hydroxyacetophenone and laminarin are used in a 1:3 mass ratio, they exhibit a synergistic effect in inhibiting the growth of molds such as Aspergillus versicolor and Penicillium purpureus, as well as bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. Compared to using hydroxyacetophenone alone, this combination enhances the inhibitory effect against various microorganisms, improves the resistance of the skin-lightening injection composition to external microorganisms, and thus extends the shelf life of the skin-lightening injection composition. Simultaneously, it reduces the amount of preservatives required in the skin-lightening injection composition, avoiding any adverse effects on human skin and health. Detailed Implementation

[0017] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0018] The skin care composition of the present invention is applied to the cleansed human face when used.

[0019] In this invention, aloe vera extract (CAS: 88507-69-3), 98%, was purchased from Shanghai Yuanye Biotechnology Co., Ltd.; steviol glycosides (CAS: 57817-89-7), 99%, were purchased from Chongqing Tianrun Biological Products Co., Ltd.; hyaluronic acid, 99%, was purchased from Hubei Nuona Technology Co., Ltd.; p-hydroxyacetophenone (CAS: 99-93-4), 98%, was purchased from Shanghai Yuanye Biotechnology Co., Ltd.; and laminarin (CAS: 9008-22-4), 98%, was purchased from (Alpha) Henan Weitixi Chemical Technology Co., Ltd.

[0020] Example 1

[0021] A preservative composition for use in skin care products, comprising p-hydroxyacetophenone and laminarin in a mass ratio of 1:3.

[0022] Example 2

[0023] A skin-lightening injection composition, by weight percentage, is prepared from the following ingredients: 1.2% aloe vera extract, 0.6% steviol glycosides, 0.04% amino acids, 1.2% hyaluronic acid, 6.2% glycerin, 0.4% 1,2-ethylene glycol, 0.15% Tween-80, 0.15% preservative composition, and the balance being sterile water; wherein the amino acids are composed of aspartic acid, phenylalanine, methionine, and taurine in a weight ratio of 4:1:2:2; the hyaluronic acid has a molecular weight of 400,000 Daltons; and the preservative composition is composed of p-hydroxyacetophenone and laminarin in a weight ratio of 1:3.

[0024] The preparation method of the above-mentioned water-light injection skin care composition is as follows: 1,2-ethylene glycol and glycerin are added to sterile water, stirred at 50 r / min for 40 min at 75℃, and then cooled to 40℃; aloe vera extract, steviol glycosides, amino acids and hyaluronic acid are added, and stirred at 50 r / min for 10 min; Tween-80 and preservative composition are added, stirred at 50 r / min for 5 min, and then cooled to room temperature.

[0025] Example 3

[0026] 1. Test strain

[0027] 1.1 Fungi

[0028] Aspergillus versicolor (AS 3.3886)

[0029] Penicillium purpurogenum (serial number: AS 3.3708)

[0030] 1.2 Bacteria

[0031] Pseudomonas aeruginosa (ATCC 9027)

[0032] Staphylococcus aureus (ATCC ID: 25923)

[0033] 2. Test reagents

[0034] 98% p-hydroxyacetophenone, 98% laminarin

[0035] The test reagent was completely dissolved in dimethyl sulfoxide and then diluted with a 0.1% Tween-80 aqueous solution to prepare a single-agent stock solution. Multiple formulations were prepared, and five mass concentration gradients were set for each single agent and each formulation mixture according to the same ratio method for later use.

[0036] 3. Test culture medium

[0037] PDA medium: 200g peeled potato, 20g glucose, 20g agar, bring to a final volume of 1000mL.

[0038] NA medium: 6g peptone, 1g yeast extract, 3g beef extract, 15g sucrose, 17g agar, add distilled water to a final volume of 1L, and adjust the pH to 7.0 with 10mol / L NaOH.

[0039] NB medium: 6g peptone, 1g yeast extract, 3g beef extract, 15g sucrose, add distilled water to a final volume of 1L, and adjust the pH to 7.0 with 10mol / L NaOH.

[0040] 4. Test methods

[0041] 4.1 Fungi

[0042] The mycelial growth rate method was used. 1 mL of the test reagent was mixed with 9 mL of melted PDA medium and poured into a sterile Petri dish to prepare a drug-containing plate. After the medium solidified, a mold mycelium (5 mm in diameter) was placed in the center of the drug-containing plate. A 0.1% Tween-80 aqueous solution was used as a control. Each treatment was repeated three times. The plates were incubated at 28±1℃ for 3 days. The diameter of the mold colony was measured using the cross-sectional method, and the inhibition rate was calculated.

[0043]

[0044] 4.2 Bacteria

[0045] The inhibition zone method was used. The test bacteria were cultured in NA medium at 30℃ for 24 h in a bacterial incubator. Two loops of colonies were picked and placed in 100 mL of NB medium, and cultured at 30℃ with shaking at 180 rpm for 24 h. The bacterial suspension concentration was adjusted to 5 × 10⁻⁶ using a hemocytometer. 7 CFU / mL. When the temperature of the sterilized NA medium drops to 50℃, pour 1 mL of the prepared bacterial suspension into 10 mL of NA medium, mix well, and then pour into a 9 cm diameter petri dish. After the medium solidifies, use an 8 mm diameter punch to make three equally spaced holes in three different directions (forming three equilateral triangles). Inject 100 μL of the same concentration of drug solution into each hole. Each treatment is repeated three times, with a blank control containing an equal volume of sterile water. Incubate the plates in a 30℃ bacterial incubator for 36 h, and measure the diameter of the inhibition zone using the cross-sectional method to calculate the inhibition rate.

[0046]

[0047] 5. Data Analysis

[0048] Using the logarithm of the drug concentration as x and the corresponding probability value of inhibition rate (or antibacterial rate) as y, a linear regression was performed to derive the virulence regression equation and the EC50 of the drug against the target pathogen. 50 The synergistic effect coefficient (SR) of the mixture was calculated using the Wadley method, and the synergistic effect of the drug mixture was evaluated based on the SR. The results are shown in Tables 1-3.

[0049] Table 1. Toxicity of the combination of p-hydroxyacetophenone and laminarin against Aspergillus versicolor.

[0050]

[0051] Table 2. Toxicity of the combination of p-hydroxyacetophenone and laminarin against *Penicillium purpurogenum*.

[0052]

[0053] Table 3. Virulence of the combination of p-hydroxyacetophenone and laminarin against Pseudomonas aeruginosa.

[0054]

[0055] Table 4. Virulence of the combination of p-hydroxyacetophenone and laminarin against Staphylococcus aureus.

[0056]

[0057] As shown in Tables 1-4, when p-hydroxyacetophenone and laminarin are used in a 1:3 mass ratio, they exhibit a synergistic effect in inhibiting the growth of molds such as Aspergillus versicolor and Penicillium purpureus, as well as bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. Compared with using p-hydroxyacetophenone alone, this combination enhances the inhibitory effect on various microorganisms, improves the resistance of the mesotherapy skincare composition to external microorganisms, and thus extends the shelf life of the mesotherapy skincare composition. Simultaneously, it reduces the amount of preservatives added to the mesotherapy skincare composition, avoiding any adverse effects on human skin and health.

[0058] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A preservative composition characterized in that, The preservative composition is composed of p-hydroxyacetophenone and laminarin in a mass ratio of 1:

3.

2. A water-light needle skin care composition characterized in that, is prepared from the following ingredients in mass percentage: aloe extract 0.6-1.8%, stevioside 0.4-0.8%, amino acid 0.02-0.06%, hyaluronic acid 0.8-1.6%, humectant 4.2-8.6%, thickening agent 0.3-0.6%, cosolvent 0.05-0.75%, the preservative composition of claim 1 0.1-0.2%, and the rest is sterile water.

3. The hydro-optical needle skin care composition according to claim 2, wherein, is prepared from the following ingredients in mass percentage: aloe extract 1.2%, stevioside 0.6%, amino acid 0.04%, hyaluronic acid 1.2%, humectant 6.2%, thickening agent 0.4%, cosolvent 0.15%, the preservative composition of claim 1 0.15%, and the rest is sterile water.

4. The hydro-optical needle skin care composition according to claim 2, wherein, The amino acid is composed of aspartic acid, phenylalanine, methionine and taurine.

5. The hydro-optical needle skin care composition according to claim 4, characterized in that, The mass ratio of the aspartic acid, phenylalanine, methionine and taurine is 4:1:2:

2.

6. The hydro-optical needle skin care composition according to claim 2, wherein, The molecular weight of the hyaluronic acid is 200-600 kilodaltons.

7. The hydro-optical needle skin care composition according to claim 2, wherein, The humectant is glycerol.

8. The hydro-optical needle skin care composition according to claim 2, wherein, The thickening agent is 1,2-ethanediol.

9. The hydro-optical needle skin care composition according to claim 2, wherein, The cosolvent is Tween-80.

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