Process for the preparation of epigallocatechin gallate by dynamic axial compression column
The two-stage separation and purification of EGCG using the dynamic axial compression column method solves the safety hazards and low purity problems in the preparation of EGCG in the existing technology, and realizes the preparation of high-purity EGCG in an efficient, safe and environmentally friendly manner, which is suitable for industrial production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 安琪生物科技有限公司
- Filing Date
- 2023-08-31
- Publication Date
- 2026-06-09
AI Technical Summary
Existing EGCG preparation technologies have safety risks, are time-consuming, and have low purity. In particular, the simulated moving bed chromatography and column chromatography resin adsorption methods use methanol as the mobile phase, which poses toxicity risks and is not very efficient.
The dynamic axial compression column method is used to purify EGCG through two-stage separation. First, a dynamic axial compression column with large-particle-size C18 packing is used for preliminary purification, and then a small-particle-size C18 packing is used for further purification. Gradient and isocratic elution are combined with non-toxic ethanol and water as mobile phases to achieve efficient separation of EGCG.
It has achieved the preparation of high-purity EGCG with a purity of over 99%, is easy to operate, suitable for industrial production, is environmentally friendly, reduces wastewater treatment costs, and has intelligent continuous production capabilities.
Smart Images
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