A stretched protein membrane and its preparation method
By introducing an auxiliary stretching membrane to adhere to the silk protein membrane before stretching and protecting the protein membrane during the stretching process, the problem of easy damage to the silk protein membrane during unilateral stretching is solved, achieving efficient stretching protection and material utilization.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
- Filing Date
- 2023-10-16
- Publication Date
- 2026-05-26
AI Technical Summary
In existing technologies, silk protein membranes are prone to cracking or damage during unilateral stretching, resulting in material waste.
Before stretching, the auxiliary stretching membrane is bonded to the protein membrane, and during the stretching process, the auxiliary stretching membrane is placed between the protein membrane and the stretching device. The auxiliary stretching membrane and the protein membrane can be separated to avoid direct contact with the clamp. High-temperature melted polyvinyl alcohol film is used as the auxiliary stretching membrane.
This effectively prevents cracks or damage to the protein membrane during stretching and fixing, thus improving the quality and utilization rate of the stretched protein membrane.
Smart Images
Figure CN117359980B_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of materials preparation technology, and in particular to a stretchable protein membrane and its preparation method. Background Technology
[0002] Silk fibroin membranes are a highly biocompatible material with advantages such as low susceptibility to immune responses, biodegradability, drug loading capacity, and excellent mechanical properties, making them an ideal choice for manufacturing medical devices. Furthermore, silk fibroin membranes can also serve as substrates for flexible electronic devices, specifically in applications such as transient soluble electronic devices.
[0003] In practical applications, stretching, fixing, and drying silk fibroin membranes can optimize and improve their performance. Furthermore, stretching protein membranes can be used to analyze the mechanical properties and characteristics of materials, such as strength, elongation, and fracture characteristics. For example, by applying tensile force, the deformation behavior of the protein membrane can be measured, as well as the mechanical property parameters of the stretched membrane, such as yield stress, ultimate stress, Young's modulus, and fracture energy.
[0004] In related technologies, the protein membrane is mainly fixed on a fixture and stretched on one side. This stretching method is very easy to cause cracks or even damage to the protein membrane. Summary of the Invention
[0005] In view of at least one technical problem in the prior art, the purpose of this application is to provide a stretchable protein membrane and a method for preparing the same.
[0006] To address the aforementioned technical problems, this application provides a method for preparing a stretched protein membrane, comprising the following steps:
[0007] Provide protein membranes;
[0008] The protein membrane is wetted to obtain a wetted protein membrane;
[0009] Provide auxiliary stretching membrane;
[0010] The auxiliary stretching membrane is bonded to any one of the wetted protein membranes to obtain the bonded protein membrane.
[0011] The bonded protein membrane is stretched to obtain a stretched protein membrane; the auxiliary stretching membrane in the stretched protein membrane can be separated from the protein membrane; and during the stretching process, the auxiliary stretching membrane is disposed between the protein membrane and the stretching device.
[0012] In some embodiments, the auxiliary stretching membrane and the protein membrane do not undergo a chemical reaction during the bonding process;
[0013] And / or, the size of the auxiliary stretching membrane is larger than the size of the protein membrane.
[0014] In some embodiments, the auxiliary stretching film is a polyvinyl alcohol film dissolved at high temperature.
[0015] In some embodiments, the step of wetting the protein membrane to obtain a wetted protein membrane includes:
[0016] The protein membrane is placed in a polar solution for wetting treatment to obtain the wetted protein membrane.
[0017] In some embodiments, the stretching treatment of the bonded protein membrane to obtain a stretched protein membrane includes:
[0018] The bonded protein membrane is stretched to obtain an intermediate bonded protein membrane;
[0019] The intermediate bonding protein membrane is dried, and the auxiliary stretching membrane is removed after drying to obtain the stretching protein membrane.
[0020] In some embodiments, stretching the bonded protein membrane to obtain an intermediate bonded protein membrane includes:
[0021] The bonded protein membrane is fixed on the first clamp; the auxiliary stretching membrane in the bonded protein membrane is close to the base plate in the first clamp, and the fixed protein membrane has a first side and a second side opposite to each other.
[0022] The first side is clamped by a clamping device, and the second side is stretched in one direction to obtain the intermediate bonded protein membrane.
[0023] In some embodiments, clamping one side using a clamping member and stretching the second side to obtain the intermediate bonded protein membrane includes:
[0024] The first side is clamped by a clamping device, and the second side is stretched in one direction to obtain an initial bonded protein membrane.
[0025] The initial bonding protein membrane is transferred to the second fixture for omnidirectional stretching to obtain the intermediate bonding protein membrane.
[0026] In some embodiments, when there are multiple intermediate bonded protein films, after stretching the bonded protein films to obtain intermediate bonded protein films, the method further includes:
[0027] Plasma treatment is performed on at least one intermediate bonding protein membrane in the direction of the protein membrane in the intermediate bonding protein membrane to obtain at least one corresponding modified bonding protein membrane.
[0028] The at least one modified bonding protein membrane is bonded a second time to an unmodified intermediate bonding protein membrane to obtain a combined protein membrane.
[0029] Accordingly, the step of drying the intermediate bonding protein membrane and removing the auxiliary stretching membrane after drying to obtain the stretching protein membrane includes:
[0030] The combined protein membrane is dried, and the auxiliary stretching membrane is removed after drying to obtain the stretching protein membrane.
[0031] In some embodiments, the auxiliary stretching membrane is bonded to any one of the wetted protein membranes to obtain a bonded protein membrane, comprising:
[0032] The auxiliary stretching film is wetted to obtain a wetted auxiliary stretching film.
[0033] Align any surface of the wetted protein membrane with the wetted auxiliary stretching membrane in water to obtain an aligned bonding membrane.
[0034] Remove the aligned bonding film and squeeze out the residual moisture in the aligned bonding film to obtain the bonded protein film.
[0035] On the other hand, this application also provides a stretchable protein membrane, which is prepared by any of the methods described in the embodiments of this application.
[0036] The stretchable protein membrane and its preparation method disclosed in this application have at least the following beneficial effects:
[0037] This application involves wetting a protein membrane to obtain a wetted protein membrane; then, attaching an auxiliary stretching membrane to any surface of the wetted protein membrane to obtain a attached protein membrane; finally, stretching the attached protein membrane to obtain a stretched protein membrane; the auxiliary stretching membrane and the protein membrane in the stretched protein membrane are separable; and during the stretching process, the auxiliary stretching membrane is positioned between the protein membrane and the stretching device. Thus, by introducing an auxiliary stretching membrane to attach to the protein membrane before stretching, the protein membrane is protected, effectively preventing cracks or even damage during stretching and fixing, and avoiding waste caused by membrane breakage. Attached Figure Description
[0038] To more clearly illustrate the technical solutions and advantages in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0039] Figure 1 This is a flowchart of a method for preparing a stretched protein membrane provided in an embodiment of this application;
[0040] Figure 2 This is a flowchart of step S105 in a method for preparing a stretched protein membrane provided in an embodiment of this application;
[0041] Figure 3 This is a flowchart of step S2013 in a method for preparing a stretched protein membrane provided in an embodiment of this application;
[0042] Figure 4 This is a schematic diagram of a structure for performing a stretching operation provided in an embodiment of this application;
[0043] Figure 5 This is a schematic diagram of a separation structure after a stretching operation is performed, provided in an embodiment of this application. Detailed Implementation
[0044] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.
[0045] The terms "an embodiment" or "embodiment" as used herein refer to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. In the description of the present invention, unless otherwise expressly specified and limited, the terms "upper," "lower," "left," "right," "top," "bottom," etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are used only for the convenience of describing the present invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of that feature. Moreover, the terms "first," "second," etc., are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of the present invention described herein can be implemented in orders other than those illustrated or described herein.
[0046] It should be understood that when describing the structure of a component, when referring to a layer or region as being "above" or "on top of" another layer or region, it can mean that it is directly above the other layer or region, or that it contains other layers or regions between it and the other layer or region. Furthermore, if the component is flipped over, the layer or region will be located "below" or "under" the other layer or region.
[0047] In practical applications, stretching, fixing, and drying silk fibroin membranes can optimize and improve their performance. Furthermore, stretching the membrane can be used to analyze the material's mechanical properties, such as strength, elongation, and fracture characteristics. For example, by applying tensile force, the deformation behavior of the membrane can be measured, as well as the mechanical property parameters after stretching, such as yield stress, ultimate stress, Young's modulus, and fracture energy. In related technologies, the membrane is mainly fixed to a fixture for unilateral stretching; this method is highly susceptible to cracking and even damage.
[0048] Therefore, this application provides a stretchable protein membrane and its preparation method. The method involves providing a protein membrane; wetting the protein membrane to obtain a wetted protein membrane; providing an auxiliary stretching membrane; bonding the auxiliary stretching membrane to any surface of the wetted protein membrane to obtain a bonded protein membrane; stretching the bonded protein membrane to obtain a stretched protein membrane; the auxiliary stretching membrane and the protein membrane in the stretched protein membrane are separable; and during the stretching process, the auxiliary stretching membrane is positioned between the protein membrane and the stretching device. Thus, by introducing an auxiliary stretching membrane to bond with the protein membrane before stretching, the protein membrane is protected, effectively preventing cracks or even damage during stretching and fixing, and avoiding waste caused by membrane breakage.
[0049] Figure 1 This is a flowchart illustrating a method for preparing a stretched protein membrane according to an embodiment of this application. The accompanying drawings are merely illustrative of common and potentially non-common aspects of multiple embodiments; differences or distinctions are presented in textual description. To reduce lengthy and unnecessary repetition of embodiments, based on industry characteristics and the nature of the technology, those skilled in the art should be able to correctly and reasonably understand and judge whether the following individual technical features or any combination thereof can characterize the same embodiment, or whether multiple mutually exclusive technical features can only characterize different variations of the embodiment. For example... Figure 1 As shown, it includes the following steps.
[0050] Step S101: Provide a protein membrane;
[0051] Step S102: Wet the protein membrane to obtain a wetted protein membrane;
[0052] Step S103: Provide an auxiliary stretching membrane;
[0053] Step S104: The auxiliary stretching membrane is bonded to any one of the membrane surfaces of the wetted protein membrane to obtain the bonded protein membrane.
[0054] Step S105: The bonded protein membrane is stretched to obtain a stretched protein membrane; the auxiliary stretching membrane and the protein membrane in the stretched protein membrane can be separated; and during the stretching process, the auxiliary stretching membrane is placed between the protein membrane and the stretching device.
[0055] Among them, the protein membrane is a cross-linked protein membrane, which can be any kind of biological protein membrane such as silk protein membrane or spider silk protein membrane, or a composite protein membrane composed of at least two protein membranes.
[0056] Specifically, taking silk fibroin membrane as an example, the preparation process of this protein membrane may include: 1) the step of preparing silk fibroin solution, which includes: degumming silkworm cocoons to form silk; dissolving the washed and dried silk in lithium bromide solution and stirring thoroughly; placing the mixed solution in an incubator for incubation; dialyzing the incubated mixed solution to obtain a silk fibroin suspension; purifying the silk fibroin suspension at a preset temperature through centrifugation, collecting the supernatant, and preparing the silk fibroin solution. 2) the mold forming step, which includes: transferring the silk fibroin solution into a mold, allowing it to stand for a period of time to form a silk fibroin membrane.
[0057] In some embodiments, step S102, which involves wetting the protein membrane to obtain a wetted protein membrane, includes: placing the protein membrane in a polar solution for wetting treatment to obtain a wetted protein membrane. Specifically, the protein membrane can be wetted by immersion in a polar solution, causing the protein membrane to swell and obtain a wetted protein membrane. The polar solvent can be an aqueous solution, an ethanol solution, or a mixture of both. The treatment time and temperature can be determined based on factors such as the thickness of the protein membrane.
[0058] In this process, the auxiliary stretching film and the protein film do not undergo a chemical reaction during lamination, and they do not stick together during preparation. That is, the auxiliary stretching film can be a suitable polymer film that does not chemically react with or stick to the protein film during lamination, and is made of a material with good mechanical properties. For example, the auxiliary stretching film can be a polyvinyl alcohol film, a polyvinylidene chloride film, etc.
[0059] Optionally, to prevent adhesion during preparation, the melting temperature of the auxiliary stretching film should be higher than the highest temperature during the preparation process. In a specific embodiment, the auxiliary stretching film can be a high-temperature soluble polyvinyl alcohol film. The melting temperature of this high-temperature soluble polyvinyl alcohol film is 85°C, which is higher than the maximum temperature of the stretching protein film during lamination or drying.
[0060] Optionally, to ensure effective protection of the protein membrane, the auxiliary stretching membrane is larger than the protein membrane. Specifically, the length and width of the auxiliary stretching membrane are both greater than the corresponding dimensions of the protein membrane. After lamination, the protein membrane is positioned at a certain distance from the four edges of the auxiliary stretching membrane, achieving full coverage protection of the entire protein membrane and improving the protection coverage rate.
[0061] In some embodiments, step S104 above, which involves bonding the auxiliary stretching membrane to any one of the wetted protein membrane surfaces to obtain the bonded protein membrane, includes:
[0062] The auxiliary stretching film is wetted to obtain the wetted auxiliary stretching film.
[0063] Align any surface of the wetted protein membrane with the wetted auxiliary stretching membrane in water to obtain an aligned laminated membrane.
[0064] Remove the aligned bonding membrane and squeeze out any residual moisture from it to obtain the bonded protein membrane.
[0065] Specifically, the auxiliary stretching film can first be wetted in a polar solution to obtain a wetted auxiliary stretching film. Specifically, the auxiliary stretching film can be wetted by immersion in a polar solution, causing it to swell, thus obtaining a wetted auxiliary stretching film. The polar solvent can be an aqueous solution, an ethanol solution, or a mixture of both. The processing time and temperature of this wettation treatment can be determined based on factors such as the thickness of the auxiliary stretching film.
[0066] Next, the moistened protein membrane and the moistened auxiliary stretching membrane are placed in water. One side of the moistened protein membrane and the moistened auxiliary stretching membrane are aligned underwater to ensure the protein membrane is completely adhered to the surface of the auxiliary stretching membrane, resulting in an aligned and bonded membrane. Then, the aligned and bonded membrane is removed from the water. Using atmospheric pressure (both are airtight) and manual operation, any residual moisture, such as excess air bubbles and water, between the two membranes is removed until they are fully bonded, resulting in a bonded protein membrane. The bond between the protein membrane and the auxiliary stretching membrane in this bonded protein membrane is achieved through hydrogen bonds and van der Waals forces.
[0067] It should be noted that during the bonding process, the temperature of the bonding environment can be controlled to be lower than the melting temperature of the auxiliary stretch film. For example, the water temperature in the bonding environment can be lower than the melting temperature of the auxiliary stretch film. For instance, the bonding operation can be performed at room temperature.
[0068] In some implementations, such as Figure 2 As shown, step S105 above involves stretching the bonded protein membrane to obtain a stretched protein membrane, including:
[0069] Step S201: Stretch the bonded protein membrane to obtain an intermediate bonded protein membrane.
[0070] Specifically, the bonded protein membrane is stretched using a stretching device to obtain an intermediate bonded protein membrane. The auxiliary stretching membrane and the protein membrane can be easily separated during the stretching process. The auxiliary stretching membrane is positioned between the protein membrane and the stretching device, for example, below the protein membrane. After bonding, both are fixed together on the stretching device for the stretching operation. During fixing, the auxiliary stretching membrane is positioned downwards and bonded to the stretching device to protect the protein membrane from stress. The stretching device may include at least one of a first clamp and a second clamp.
[0071] In some embodiments, stretching the bonded protein membrane to obtain an intermediate bonded protein membrane includes:
[0072] Step S2011: Fix the bonded protein membrane onto the first clamp; the auxiliary stretching membrane in the bonded protein membrane is close to the base plate in the first clamp, and the fixed protein membrane has a first side and a second side.
[0073] In step S2013, the first side is clamped by the clamping member, and the second side is stretched in one direction to obtain the intermediate bonded protein membrane.
[0074] The first clamp can be a clamp for unidirectional tension, which may include a base plate and a clamping element.
[0075] Specifically, the bonded protein membrane can be fixed to a first clamp. The base plate of the first clamp can be provided with a groove that opens along the stretching direction, so that the bonded protein membrane can be placed in the groove, and the first side of the bonded protein membrane is fixed with a clamping member. The auxiliary stretching membrane in the bonded protein membrane is close to the base plate of the first clamp, and the fixed protein membrane has opposing first and second sides. The first side is clamped by the clamping member, and the second side is stretched in one direction to obtain the intermediate bonded protein membrane.
[0076] Step S203: Dry the intermediate bonding protein membrane and remove the auxiliary stretching membrane after drying to obtain the stretching protein membrane.
[0077] Specifically, the intermediate bonding protein membrane is dried to obtain a dried bonding protein membrane. The auxiliary stretching membrane and the protein membrane in the dried bonding protein membrane are separated, and the auxiliary stretching membrane is removed to obtain the stretching protein membrane. After drying and separation, the obtained stretching protein membrane has no residue.
[0078] In some implementations, such as Figure 3 As shown, step S2013 above, which clamps the first side with a clamping member and stretches the second side to obtain the intermediate bonded protein membrane, includes:
[0079] Step S301: The first side is clamped by the clamping member, and the second side is stretched in one direction to obtain the initial adhered protein membrane.
[0080] Step S303: The initial bonding protein membrane is transferred to the second clamp for omnidirectional stretching to obtain the intermediate bonding protein membrane.
[0081] Specifically, the first side is clamped by the clamping element in the first fixture, while only the second side is stretched in one direction to obtain an initial bonded protein film. Next, the initial bonded protein film is transferred to the second fixture for omnidirectional stretching to obtain an intermediate bonded protein film. This second fixture can be an omnidirectionally fixed fixture, such as an embroidery hoop or a similar fixture structure. In this way, by assisting in stretching the film, cracks or damage to the protein film during transfer to the second fixture can be avoided.
[0082] In some embodiments, when there are multiple intermediate bonded protein films, after stretching the bonded protein films to obtain intermediate bonded protein films, the method further includes:
[0083] Plasma treatment is performed on at least one intermediate bonded protein membrane in the direction of the protein membrane in the intermediate bonded protein membrane to obtain at least one corresponding modified bonded protein membrane.
[0084] At least one modified bonding protein membrane is bonded to an unmodified intermediate bonding protein membrane in a second bonding process to obtain a combined protein membrane.
[0085] Specifically, when there are multiple intermediate bonded protein films, each protein film can be wetted and bonded separately to obtain multiple intermediate bonded protein films. For at least one intermediate bonded protein film, it can be subjected to plasma treatment in the direction of the protein film in the intermediate bonded protein film to obtain at least one corresponding modified bonded protein film. Then, the at least one modified bonded protein film is bonded to an unmodified intermediate bonded protein film to achieve bonding between the film surfaces of the two protein films, resulting in a combined protein film.
[0086] It should be noted that if an unmodified intermediate bonding protein membrane is not available, two modified bonding protein membranes can be bonded together a second time to obtain a combined protein membrane.
[0087] The aforementioned plasma treatment of the intermediate bonding protein membrane allows for the formation of more hydroxyl groups on the membrane surface, enhancing efficient cross-linking with other proteins and improving the adhesion between the protein membranes. Furthermore, during the plasma treatment process, the auxiliary stretching membrane effectively protects the protein membrane surface, preventing damage.
[0088] Accordingly, the intermediate bonding protein membrane is dried, and the auxiliary stretching membrane is removed after drying to obtain a stretched protein membrane comprising:
[0089] The combined protein membrane was dried, and the auxiliary stretching membrane was removed after drying to obtain the stretched protein membrane.
[0090] Specifically, the combined protein membrane is dried to obtain a dried combined protein membrane. The auxiliary stretching membrane and the protein membrane in the dried combined protein membrane are then separated, and the auxiliary stretching membrane is removed to obtain the stretched protein membrane. After drying and separation, the obtained stretched protein membrane has no residue. Thus, by combining protein membranes, the versatility of the stretched protein membrane's properties is improved to meet the needs of different application scenarios.
[0091] In practical applications, such as Figure 4 As shown, taking the stretching device including the first clamp as an example, the protein membrane 41 is attached to the auxiliary stretching membrane 42, and both are fixed together to the base plate 43 in the first clamp. One side is clamped by the clamping member 44 (e.g., a clip) in the first clamp, and the other side is stretched. Figure 5 As shown, the stretched protein membrane 41 and the auxiliary stretching membrane 42 can be easily separated by tweezers, while keeping the stretched protein membrane 41 from cracking or breaking.
[0092] The experimental results show that protein membranes without auxiliary stretching membrane protection are likely to develop large cracks after being stretched, while protein membranes using auxiliary stretching membrane as a protective measure have a significantly reduced probability of breaking, making the stretching of protein membranes more effective.
[0093] The above method protects the protein membrane by introducing an auxiliary stretching membrane to adhere to the protein membrane before stretching. This effectively prevents cracks or damage to the protein membrane during stretching and fixing, thus avoiding waste caused by membrane breakage.
[0094] Furthermore, as protein membranes are biological products, they contain some uneven defects that can easily become stress concentration points. In addition, there are stress concentration points such as sharp protrusions introduced by stretching devices like clamps. During stretching, transfer, and plasma treatment, the stress in the protein membrane increases, and these stress concentration points are prone to damage, potentially leading to the destruction of the entire membrane. In this case, the presence of an auxiliary stretching membrane, which has a certain adhesive force with the protein membrane, can distribute the stress at these stress concentration points, preventing damage to the protein membrane. This adhesive force comes partly from hydrogen bonds and partly from van der Waals forces, eliminating the need for additional adhesives. Moreover, after the protein membrane has undergone stretching and other operations, and after drying, the auxiliary stretching membrane can be easily separated from the protein membrane with virtually no residue, thus not affecting subsequent testing of the stretched protein membrane.
[0095] This application also provides a stretchable protein membrane, which is prepared by any of the stretchable protein membrane preparation methods described above.
[0096] Thus, by introducing an auxiliary stretching membrane to adhere to the protein membrane before stretching, the protein membrane is protected, effectively preventing cracks or even damage during stretching and fixing, avoiding waste caused by protein membrane breakage, and improving the quality of the stretched protein membrane.
[0097] It should be noted that the specific details and beneficial effects of the above device embodiments can be found in the above method embodiments, and will not be repeated here.
[0098] The foregoing description has fully disclosed the specific embodiments of this application. It should be noted that any modifications made by those skilled in the art to the specific embodiments of this application do not depart from the scope of the claims. Accordingly, the scope of the claims of this application is not limited to the foregoing specific embodiments.
Claims
1. A method for preparing a stretched protein membrane, characterized in that, Includes the following steps: Provide protein membranes; The protein membrane is wetted to obtain a wetted protein membrane; Provide auxiliary stretching membrane; The auxiliary stretching membrane is bonded to any one of the wetted protein membranes to obtain the bonded protein membrane. The bonded protein membrane is stretched to obtain a stretched protein membrane. The auxiliary stretching membrane and the protein membrane in the stretching protein membrane can be separated; and during the stretching process, the auxiliary stretching membrane is disposed between the protein membrane and the stretching device.
2. The method according to claim 1, characterized in that, The auxiliary stretching membrane and the protein membrane do not undergo a chemical reaction during the bonding process; And / or, the size of the auxiliary stretching membrane is larger than the size of the protein membrane.
3. The method according to claim 2, characterized in that, The auxiliary stretching membrane is a polyvinyl alcohol film dissolved at high temperature.
4. The method according to claim 1, characterized in that, The step of wetting the protein membrane to obtain a wetted protein membrane includes: The protein membrane is placed in a polar solution for wetting treatment to obtain the wetted protein membrane.
5. The method according to claim 1, characterized in that, The stretching process of the bonded protein membrane to obtain the stretched protein membrane includes: The bonded protein membrane is stretched to obtain an intermediate bonded protein membrane; The intermediate bonding protein membrane is dried, and the auxiliary stretching membrane is removed after drying to obtain the stretching protein membrane.
6. The method according to claim 5, characterized in that, The step of stretching the bonded protein membrane to obtain the intermediate bonded protein membrane includes: The bonded protein membrane is fixed on the first clamp; the auxiliary stretching membrane in the bonded protein membrane is close to the base plate in the first clamp, and the fixed protein membrane has a first side and a second side opposite to each other. The first side is clamped by a clamping device, and the second side is stretched in one direction to obtain the intermediate bonded protein membrane.
7. The method according to claim 6, characterized in that, The step of clamping the first side with a clamping member and stretching the second side to obtain the intermediate bonded protein membrane includes: The first side is clamped by a clamping device, and the second side is stretched in one direction to obtain an initial adhered protein membrane; The initial bonding protein membrane is transferred to the second fixture for omnidirectional stretching to obtain the intermediate bonding protein membrane.
8. The method according to claim 5, characterized in that, When there are multiple intermediate bonded protein films, after stretching the bonded protein films to obtain intermediate bonded protein films, the method further includes: Plasma treatment is performed on at least one intermediate bonding protein membrane in the direction of the protein membrane in the intermediate bonding protein membrane to obtain at least one corresponding modified bonding protein membrane. The at least one modified bonding protein membrane is bonded a second time to an unmodified intermediate bonding protein membrane to obtain a combined protein membrane. Accordingly, the step of drying the intermediate bonding protein membrane and removing the auxiliary stretching membrane after drying to obtain the stretching protein membrane includes: The combined protein membrane is dried, and the auxiliary stretching membrane is removed after drying to obtain the stretching protein membrane.
9. The method according to any one of claims 1-8, characterized in that, The auxiliary stretching membrane is bonded to any one of the surfaces of the moistened protein membrane to obtain the bonded protein membrane, comprising: The auxiliary stretching film is wetted to obtain a wetted auxiliary stretching film. Align any surface of the wetted protein membrane with the wetted auxiliary stretching membrane in water to obtain an aligned bonding membrane. Remove the aligned bonding film and squeeze out the residual moisture in the aligned bonding film to obtain the bonded protein film.
10. A stretchable protein membrane, characterized in that, The stretching protein membrane is prepared by the method described in any one of claims 1-9.