A culture medium for rapid cultivation of Panax notoginseng adventitious roots and a method for rapid cultivation of Panax notoginseng adventitious roots

Abstract

The present application relates to the technical field of Panax notoginseng cultivation, and specifically discloses a culture medium for rapid cultivation of Panax notoginseng adventitious roots and a method for rapid cultivation of Panax notoginseng adventitious roots. The culture medium includes 1-8 Vol.% of giant fungus grass root exosome fluid, which is obtained by dispersing giant fungus grass root exosomes in magnetized water, and the content of giant fungus grass root exosomes in the giant fungus grass root exosome fluid is 20-120 mg / L. The culture medium of the present application can be used for inducing adventitious roots of Panax notoginseng, and it has a high adventitious root induction rate after cultivating Panax notoginseng materials from different sources.

Description

Technical Field

[0001] The present application relates to the technical field of Panax notoginseng cultivation, and more specifically, to a culture medium for rapid cultivation of Panax notoginseng adventitious roots and a method for rapid cultivation of Panax notoginseng adventitious roots. Background Art

[0002] Panax notoginseng is a plant of the genus Panax in the Araliaceae family. It has the functions of stopping bleeding, replenishing blood, strengthening the body, and treating coronary heart disease and cerebrovascular diseases. It mainly grows in Wenshan, Yunnan and the adjacent Zuoyoujiang area of ​​Guangxi. Due to its excellent medicinal effects, the market demand for Panax notoginseng is also increasing. However, the traditional planting method has problems such as long growth cycle of Panax notoginseng, serious diseases and insect pests, and "old Panax notoginseng land", which limits the sustainable development of the Panax notoginseng industry.

[0003] When cultivating Panax notoginseng using plant tissue culture technology, this method has the advantages of less land demand and the sterile culture environment leads to fewer diseases and insect pests. Therefore, plant tissue culture is currently a more mainstream direction in the research and development of Panax notoginseng cultivation.

[0004] In the related art, when adventitious roots are induced by explants, the explants are placed in a culture medium for dark culture for 60 days, and the culture medium components are: 3mg / L IBA, 0.1mg / L KT, MS culture medium. When adventitious roots are induced by callus tissue, the callus tissue is placed in a culture medium for dark culture for 5 weeks, and the culture medium components are: 3mg / L IBA, 0.1mg / L KT, 1 / 2MS culture medium. When adventitious roots are induced by regenerated seedling explants, the regenerated seedling explants are placed in a culture medium for dark culture for 5 weeks, and the culture medium components are: 3mg / L IBA, 0.1mg / L KT, 1 / 2MS culture medium.

[0005] In the above methods, when adventitious roots are induced by callus tissue, the induction rate of adventitious roots is less than 50% when the callus tissue from leaves, petioles and stem segments is induced, and the adventitious root induction rate of callus tissue from flower buds and fibrous roots is only 90-95%. When adventitious roots are induced by root hairs as explants, the induction rate of adventitious roots is only about 46%.

[0006] Therefore, it is necessary to provide a culture medium and a method to obtain a high adventitious root induction rate. Summary of the invention

[0007] In order to improve the induction rate of adventitious roots during tissue culture of Panax notoginseng, the present application provides a culture medium for rapid culture of Panax notoginseng adventitious roots and a method for rapid culture of Panax notoginseng adventitious roots.

[0008] In a first aspect, the present application provides a culture medium for rapid cultivation of adventitious roots of Panax notoginseng, using the following technical solution:

[0009] A culture medium for rapid culture of Panax notoginseng adventitious roots, the culture medium being an adventitious root culture medium for callus or an adventitious root culture medium for explants;

[0010] The culture medium comprises 1-8 Vol.% of giant fungus grass root exosome fluid, which is obtained by dispersing giant fungus grass root exosomes in magnetized water, and the content of giant fungus grass root exosomes in the giant fungus grass root exosome fluid is 20-120 mg / L.

[0011] Giant fungus grass has a developed root system, and its root exosomes contain more substances that promote rooting. By adopting the above technical scheme, the present application first adds giant fungus grass root exosomes to a culture medium with a root-inducing effect, so as to promote the induction of adventitious roots through the exosomes and increase the adventitious root induction rate. In addition, in this scheme, the exosomes are dispersed with magnetized water with a certain exogenous energy at the same time, in the hope of affecting the exosomes through magnetized water; at the same time, the magnetized water further affects the adventitious root induction of the Panax notoginseng material. Under the interaction of giant fungus grass root exosomes and magnetized water and the synergistic effect of the two on the Panax notoginseng material, the adventitious root induction rate of the Panax notoginseng material is significantly improved.

[0012] Optionally, when the culture medium is an adventitious root culture medium for callus tissue, the culture medium further comprises the following components: 1 / 2MS agar medium, 2.0-4.0 mg / L IBA and 0.05-0.15 mg / L KT.

[0013] In this application, IBA refers to indolebutyric acid and KT refers to kinetin.

[0014] By adopting the above technical solution, the culture medium has an excellent rooting promoting effect, and when the culture medium is used for adventitious root culture, a better adventitious root induction effect can be obtained.

[0015] Optionally, when the culture medium is an adventitious root culture medium for explants, the culture medium further comprises the following components: MS agar medium, 2.0-4.0 mg / L IBA and 0.05-0.15 mg / L KT.

[0016] By adopting the above technical solution, the culture medium has an excellent rooting promoting effect, and when the culture medium is used for adventitious root culture, a better adventitious root induction effect can be obtained.

[0017] Optionally, the method for preparing exosomes in the exosome fluid of giant fungus grass roots comprises the following steps:

[0018] Step I: clean the roots of giant fungus grass, crush them and grind them into slurry, and obtain juice after solid-liquid separation;

[0019] Step II: subjecting the juice to differential centrifugation to obtain exosomes from the roots of giant fungus grass.

[0020] By adopting the above technical scheme, the exosomes of giant fungus grass roots with relatively high purity can be obtained.

[0021] Optionally, the differential centrifugation in step II includes the following steps: centrifuging the juice at a speed of 2500-3500 rpm for 15-25 min; dispersing the precipitate with water, and then centrifuging at a speed of 9000-11000 rpm for 30-50 min; dispersing the precipitate with water, and finally centrifuging at a speed of 14000-16000 rpm for 100-130 min.

[0022] By adopting the above technical solution, the exosomes of giant fungus grass roots with higher purity are obtained by differential centrifugation.

[0023] Optionally, the method for preparing magnetized water comprises the following steps: obtaining the magnetized water by magnetizing water at a magnetic field strength of 0.5-1.5 T for 60-150 min.

[0024] By adopting the above technical solution, magnetized water is obtained in this method, which contains an appropriate amount of energy; this energy will not excessively affect the stable existence of giant grass root exosomes, and can also interact with the giant grass root exosomes and Panax notoginseng materials, thereby exerting an excellent effect of promoting the induction of adventitious roots.

[0025] In a second aspect, the present application provides a method for rapid cultivation of adventitious roots of Panax notoginseng, which adopts the following technical scheme: a method for rapid cultivation of adventitious roots of Panax notoginseng, the method comprising the steps of placing Panax notoginseng material on the above-mentioned culture medium for rapid cultivation of adventitious roots of Panax notoginseng to induce rooting;

[0026] When the Panax notoginseng material is Panax notoginseng callus, the method comprises the step of placing the Panax notoginseng callus on an adventitious root culture medium for the callus to induce rooting;

[0027] Alternatively, when the Panax notoginseng material is a Panax notoginseng explant, the method includes the step of placing the Panax notoginseng explant on an adventitious root culture medium for the explant to induce rooting.

[0028] Optionally, when the Panax notoginseng callus tissue is used as the Panax notoginseng material, the culture conditions include: dark culture at 22-28°C for 20-30 days; when the Panax notoginseng explant is used as the Panax notoginseng material, the culture conditions include: dark culture at 22-28°C for 25-70 days.

[0029] Optionally, the Panax notoginseng explants are selected from any one or more of Panax notoginseng leaves, Panax notoginseng roots, Panax notoginseng young leaves, Panax notoginseng stem segments, roots of Panax notoginseng callus-induced regenerated seedlings, rooted stem segments of Panax notoginseng callus-induced regenerated seedlings, stem segments of Panax notoginseng callus-induced regenerated seedlings and young leaves of Panax notoginseng callus-induced regenerated seedlings.

[0030] In summary, this application has the following beneficial effects:

[0031] 1. This application adds giant fungus grass exosomes to the root induction medium to significantly improve the root induction effect of Panax notoginseng explants or callus tissue. The giant fungus grass exosomes are dispersed in magnetized water and added to the culture medium, and the effect of the exosomes is significantly improved by the influence and strengthening effect of the magnetized water on the exosomes.

[0032] 2. The magnetized water in the giant fungus grass exosome fluid prepared in the present application is recommended to be prepared by an appropriate method, especially the magnetic field strength is recommended to be in the range of 0.5-1.5T. DETAILED DESCRIPTION

[0033] The present application is further described in detail below in conjunction with the examples. It is particularly noted that if no specific conditions are specified in the following examples, the experiments are carried out according to conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, the raw materials used in the following examples can be obtained from common commercial sources.

[0034] In the present application, IBA refers to 6-benzylaminoadenine, 2,4-D refers to 2,4-dichlorophenoxyacetic acid, and KT refers to kinetin.

[0035] Preparation Example

[0036] The following preparation examples 1-4 are preparation examples of exosome fluid from the roots of giant fungus grass.

[0037] Preparation Example 1

[0038] The preparation method of the giant fungus grass root exosome fluid is as follows: disperse the giant fungus grass root exosomes in magnetized water so that the content of the giant fungus grass root exosomes is 20 mg / L, and the giant fungus grass root exosome fluid is obtained after uniform dispersion.

[0039] Among them, the preparation method of giant fungus grass root exosomes is:

[0040] Step I: Take the root of giant fungus grass and rinse it with tap water to remove the soil, dust and other obvious large particles on the root, then rinse the root of giant fungus grass with washing powder water, and then use tap water to clean the residual washing powder. Then chop the clean giant fungus grass root, grind it into a slurry, filter it and discard the solid waste to obtain juice.

[0041] Step II, obtain the exosomes of the giant fungus grass root after differential centrifugation of the juice, the specific steps are as follows: centrifuge the juice at 2500rpm for 25min; take the precipitate and disperse it evenly with water, and then centrifuge it at 9000rpm for 50min; take the precipitate and disperse it evenly with water again, and finally centrifuge it at 14000rpm for 130min. The precipitate is the exosomes of the giant fungus grass.

[0042] The preparation method of magnetized water is as follows: magnetize water at a magnetic field strength of 0.5 T for 150 minutes to obtain the magnetized water.

[0043] Preparation Example 2

[0044] The preparation method of the giant fungus grass root exosome fluid is as follows: disperse the giant fungus grass root exosomes in magnetized water so that the content of the giant fungus grass root exosomes is 80 mg / L, and obtain the giant fungus grass root exosome fluid after uniform dispersion.

[0045] Among them, the preparation method of giant fungus grass root exosomes is:

[0046] Step I: Take the root of giant fungus grass and rinse it with tap water to remove the soil, dust and other obvious large particles on the root, then rinse the root of giant fungus grass with washing powder water, and then use tap water to clean the residual washing powder. Then chop the clean giant fungus grass root, grind it into a slurry, filter it and discard the solid waste to obtain juice.

[0047] Step II, obtain the exosomes of the giant fungus grass root after differential centrifugation of the juice, the specific steps are as follows: centrifuge the juice at 3000rpm for 20min; take the precipitate and disperse it evenly with water, and then centrifuge it at 10000rpm for 40min; take the precipitate and disperse it evenly with water again, and finally centrifuge it at 15000rpm for 120min. The precipitate is the exosomes of the giant fungus grass.

[0048] The preparation method of magnetized water is as follows: magnetize water at a magnetic field strength of 1.0 T for 100 minutes.

[0049] Preparation Example 3

[0050] The preparation method of the giant fungus grass root exosome fluid is as follows: disperse the giant fungus grass root exosomes in magnetized water so that the content of the giant fungus grass root exosomes is 120 mg / L, and obtain the giant fungus grass root exosome fluid after uniform dispersion.

[0051] Among them, the preparation method of giant fungus grass root exosomes is:

[0052] Step I: Take the root of giant fungus grass and rinse it with tap water to remove the soil, dust and other obvious large particles on the root, then rinse the root of giant fungus grass with washing powder water, and then use tap water to clean the residual washing powder. Then chop the clean giant fungus grass root, grind it into a slurry, filter it and discard the solid waste to obtain juice.

[0053] Step II, obtain the exosomes of the giant fungus grass root after differential centrifugation of the juice, the specific steps are as follows: centrifuge the juice at 3500rpm for 15min; take the precipitate and disperse it evenly with water, and then centrifuge it at 11000rpm for 30min; take the precipitate and disperse it evenly with water again, and finally centrifuge it at 16000rpm for 100min. The precipitate is the exosomes of the giant fungus grass.

[0054] The preparation method of magnetized water is as follows: magnetize water at a magnetic field strength of 1.5 T for 60 minutes to obtain the magnetized water.

[0055] Preparation Example 4

[0056] The difference from Preparation Example 2 is that the exosome liquid of the root of giant fungus grass in this Preparation Example is obtained by dispersing the exosomes of the root of giant fungus grass in water.

[0057] Specifically, the preparation method of the giant fungus grass root exosome fluid is: disperse the giant fungus grass root exosomes in water so that the content of the giant fungus grass root exosomes is 80 mg / L, and the giant fungus grass root exosome fluid is obtained after uniform dispersion.

[0058] Among them, the preparation method of giant fungus grass root exosomes is the same as Preparation Example 2.

[0059] Preparation Example 5-8

[0060] The difference between Preparation Examples 5-8 and Preparation Example 2 is that the preparation methods of magnetized water are different, as follows:

[0061] In Preparation Example 5, the preparation method of magnetized water is as follows: magnetizing water at a magnetic field strength of 0.2 T for 100 min to obtain the magnetized water;

[0062] In Preparation Example 6, the preparation method of magnetized water is as follows: magnetizing water at a magnetic field strength of 0.5 T for 100 min to obtain the magnetized water;

[0063] In Preparation Example 7, the preparation method of magnetized water is as follows: magnetizing water at a magnetic field strength of 1.5 T for 100 minutes to obtain;

[0064] In Preparation Example 8, the preparation method of magnetized water is: magnetize water at a magnetic field strength of 2.0 T for 100 minutes to obtain the magnetized water.

[0065] Examples and Comparative Examples

[0066] Examples 1-10 and Comparative Examples 1-4 are examples of inducing adventitious roots using Panax notoginseng explants grown in the field as Panax notoginseng materials, and are specifically as follows:

[0067] Example 1

[0068] A medium for rapid cultivation of Panax notoginseng adventitious roots, specifically an adventitious root medium for explants, the components of the medium are: MS agar medium (agar content is 0.5wt%), 2.0mg / L IBA, 0.15mg / L KT and 1Vol.% of giant fungus grass root exosome fluid. The giant fungus grass root exosome fluid is prepared by Preparation Example 1.

[0069] A method for rapidly cultivating adventitious roots of Panax notoginseng, comprising the following specific steps:

[0070] 1) Prepare explants: Take Panax notoginseng cultivated in the field, first rinse the surface dust and large particles with tap water, then soak it in detergent water for 1 hour, then rinse the residual detergent with tap water, and then use filter paper to absorb excess water on the plant surface. Cut out the roots of Panax notoginseng, disinfect them with 75Vol.% ethanol solution for 30s, then rinse them with sterile water; then disinfect them in 1wt% NaClO solution for 10min, rinse the residual NaClO with sterile water, and then use filter paper to absorb the water, cut them into appropriate sizes, and set aside.

[0071] 2) Adventitious root cultivation: Place the roots treated in 1) on an adventitious root culture medium for explants and induce cultivation in the dark at 28°C for 50 days.

[0072] Example 2

[0073] A medium for rapid cultivation of Panax notoginseng adventitious roots, specifically an adventitious root medium for explants, the components of the medium are: MS agar medium (agar content is 0.5wt%), 3.0mg / L IBA, 0.10mg / L KT and 5Vol.% of giant fungus grass root exosome fluid. The giant fungus grass root exosome fluid is prepared by Preparation Example 2.

[0074] A method for rapidly cultivating adventitious roots of Panax notoginseng, comprising the following specific steps:

[0075] 1) Prepare explants. The specific steps are the same as step 1) of Example 1.

[0076] 2) Adventitious root cultivation: Place the roots treated in 1) on an adventitious root culture medium for explants and induce cultivation in the dark at 25°C for 60 days.

[0077] Example 3

[0078] A medium for rapid cultivation of Panax notoginseng adventitious roots, specifically an adventitious root medium for explants, the components of the medium are: MS agar medium (agar content is 0.5wt%), 4.0mg / L IBA, 0.15mg / L KT and 8Vol.% of giant fungus grass root exosome fluid. The giant fungus grass root exosome fluid is prepared by Preparation Example 3.

[0079] A method for rapidly cultivating adventitious roots of Panax notoginseng, comprising the following specific steps:

[0080] 1) Prepare explants. The specific steps are the same as step 1) of Example 1.

[0081] 2) Adventitious root cultivation: Place the roots treated in 1) on an adventitious root culture medium for explants and culture them in the dark at 22°C for 70 days.

[0082] Embodiment 4-5

[0083] The difference between this embodiment and embodiment 2 is that the volume percentage of the exosome fluid of the giant fungus grass root in the adventitious root culture medium for explants is different. Specifically: 1 Vol.% in embodiment 4 and 8 Vol.% in embodiment 5, and the rest is the same as embodiment 2.

[0084] Example 6

[0085] The difference between this embodiment and embodiment 2 is that the explant for adventitious root induction is the petiole of Panax notoginseng planted in the field, and the rest is the same as embodiment 2.

[0086] Examples 7-10

[0087] The difference between Examples 7-10 and Example 2 is that the sources of the exosome fluid from the roots of giant fungus grass are different, as follows:

[0088] In Example 7, the exosome fluid of the root of giant fungus grass was prepared by Preparation Example 5;

[0089] In Example 8, the exosome fluid of the root of giant fungus grass was prepared by Preparation Example 6;

[0090] In Example 9, the exosome fluid of the root of giant fungus grass was prepared by Preparation Example 7;

[0091] In Example 10, the exosome fluid of giant fungus grass roots was prepared according to Preparation Example 8.

[0092] Comparative Example 1

[0093] The difference between this comparative example and Example 2 is that the exosome fluid of the root of giant fungus grass in the adventitious root culture medium for explants is prepared by Preparation Example 4.

[0094] Comparative Example 2

[0095] The difference between this comparative example and Example 2 is that the adventitious root culture medium for explants does not contain the exosome fluid of the root of giant fungus grass, and the rest is the same as Example 2.

[0096] Comparative Example 3

[0097] The difference between this comparative example and Example 6 is that the exosome fluid of the giant fungus grass root in the adventitious root culture medium for explants is prepared by Preparation Example 4, and the rest is the same as Example 6.

[0098] Comparative Example 4

[0099] The difference between this comparative example and Example 6 is that the adventitious root culture medium for explants does not contain the exosome fluid of the root of giant fungus grass, and the rest is the same as Example 6.

[0100] The adventitious root induction conditions of Examples 1-10 and Comparative Examples 1-4 were statistically analyzed, and the specific results are shown in Table 1. Wherein, adventitious root induction rate (%) = number of adventitious root induced explants / number of inoculated explants × 100%; the number of inoculated explants is 50.

[0101] Table 1 Adventitious root induction of Panax notoginseng explants in different implementation schemes

[0102]

[0103] From the comparison of the results of Examples 1-3 (especially Example 2) and Comparative Example 2 in Table 1, after adding the giant fungus grass exosome fluid to the culture medium for inducing rooting, the adventitious root induction rate of Panax notoginseng roots can be significantly improved. And further combined with the data of Comparative Example 1, it is found that compared with the giant fungus grass exosomes dispersed in water, the giant fungus grass exosome fluid prepared by dispersing the giant fungus grass exosomes with magnetized water is added to the culture medium for inducing rooting to induce adventitious roots of Panax notoginseng roots, and the adventitious root induction rate can be further improved. And Examples 7-10 also further explored the effect of the magnetized water preparation method on the adventitious root induction of Panax notoginseng roots. The results show that when preparing magnetized water, the magnetic field strength is recommended to be in the range of 0.5-1.5T to ensure a better adventitious root induction rate of Panax notoginseng roots. In addition, Examples 4-5 show that when the amount of giant fungus grass root exosome fluid added to the culture medium for inducing rooting is 1-8Vol.%, the culture medium can achieve a higher adventitious root induction rate of Panax notoginseng roots.

[0104] Example 6 and Comparative Examples 3-4 prove that when adventitious roots are induced by the petiole of Panax notoginseng, the conclusion that "adding the exosome fluid of Giant Juncao to the culture medium for inducing rooting can significantly increase the adventitious root induction rate of the petiole of Panax notoginseng" can also be drawn. In the above scheme, the exosome fluid of Giant Juncao is recommended to be dispersed with magnetized water.

[0105] Examples 11-15 and Comparative Examples 5-10 are examples of inducing adventitious roots using Panax notoginseng callus as the Panax notoginseng material, and the details are as follows:

[0106] Embodiment 11

[0107] A medium for rapid culture of Panax notoginseng adventitious roots, specifically an adventitious root medium for callus tissue, the components of the medium are: 1 / 2MS agar medium (agar content is 0.5wt%), 2.0mg / L IBA, 0.15mg / L KT and 5Vol.% of giant fungus grass root exosome fluid. The giant fungus grass root exosome fluid is prepared by Preparation Example 2.

[0108] A method for rapidly cultivating adventitious roots of Panax notoginseng, comprising the following specific steps:

[0109] ①. Preparation of callus tissue

[0110] Take the Panax notoginseng plant grown in the field, first rinse the surface dust and large particles with tap water, then soak it in detergent water for 1 hour, then rinse the residual detergent with tap water, and then use filter paper to absorb excess water on the plant surface. Cut out the roots of Panax notoginseng, disinfect them with 75Vol.% ethanol solution for 30s, then rinse them with sterile water; then disinfect them in 1wt% NaClO solution for 10min, rinse the residual NaClO with sterile water, and then use filter paper to absorb the water, cut them into appropriate sizes, and set aside.

[0111] The roots were placed in a callus induction medium for induction culture for 35 days, the obtained callus was taken out, and then placed in a new callus medium for subculture for 6 months, and the obtained callus was used for standby. The components of the callus induction medium are: MS agar medium, 2 mg / L 2,4-D and 0.7 mg / L KT.

[0112] ②. Cultivation of adventitious roots: Place the callus derived from the roots treated in step ① on adventitious root culture medium for callus tissue and induce culture in the dark at 28°C for 25 days.

[0113] Examples 12-15

[0114] The difference between Examples 12-15 and Example 11 is that the materials for inducing callus are different, as follows:

[0115] In Example 12, after inducing callus tissue from Panax notoginseng flower buds, adventitious roots were induced using the callus tissue derived from the flower buds;

[0116] In Example 13, after inducing callus tissue from Panax notoginseng leaves, adventitious roots were induced using the callus tissue derived from the leaves;

[0117] In Example 14, after inducing callus tissue from the petiole of Panax notoginseng, adventitious roots were induced from the callus tissue derived from the petiole;

[0118] In Example 15, after callus tissue was induced from Panax notoginseng stem segments, adventitious roots were induced using the callus tissue derived from the stem segments.

[0119] Comparative Examples 5-6

[0120] The difference between Comparative Examples 5-6 and Example 13 is that the components in the adventitious root culture medium for callus tissue are different; specifically, the giant fungus grass root exosome fluid in the adventitious root culture medium for callus tissue in Comparative Example 5 is prepared by Preparation Example 4, and the giant fungus grass root exosome fluid is not added to the adventitious root culture medium for callus tissue in Comparative Example 6.

[0121] Comparative Examples 7-8

[0122] The difference between Comparative Examples 7-8 and Example 14 is that the components in the adventitious root culture medium for callus tissue are different; specifically, the giant fungus grass root exosome fluid in the adventitious root culture medium for callus tissue in Comparative Example 7 is prepared by Preparation Example 4, and the giant fungus grass root exosome fluid is not added to the adventitious root culture medium for callus tissue in Comparative Example 8.

[0123] Comparative Examples 9-10

[0124] The difference between Comparative Examples 9-10 and Example 15 is that the components in the adventitious root culture medium for callus tissue are different; specifically, the giant fungus grass root exosome fluid in the adventitious root culture medium for callus tissue in Comparative Example 9 is prepared by Preparation Example 4, and the giant fungus grass root exosome fluid is not added to the adventitious root culture medium for callus tissue in Comparative Example 10.

[0125] The adventitious root induction conditions of Examples 11-15 and Comparative Examples 5-10 were statistically analyzed, and the specific results are shown in Table 1. Wherein, adventitious root induction rate (%) = number of adventitious root induction explants / number of inoculated explants × 100%; the number of inoculated explants is 50.

[0126] Table 2 Induction of adventitious roots of Panax notoginseng callus from different sources in different implementation schemes

[0127]

[0128] From the data results of Examples 11-15 in Table 2, it can be seen that when the callus tissues are derived from the roots, flower buds, leaves, petioles and stem segments of Panax notoginseng planted in the field, the above callus tissues are cultured with the root induction medium of the present application, and excellent root induction effects can be obtained. In addition, by comparing the results of Example 13 with Comparative Examples 5-6, it can be seen that the method of the present application can significantly improve the adventitious root induction rate of callus tissue from leaves; by comparing the results of Example 14 with Comparative Examples 7-8, it can be seen that the method of the present application can significantly improve the adventitious root induction rate of callus tissue from petioles; by comparing the results of Example 15 with Comparative Examples 9-10, it can be seen that the method of the present application can significantly improve the adventitious root induction rate of callus tissue from stem segments.

[0129] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.

Claims

1. Use of a medium for rapid cultivation of Panax notoginseng adventitious roots in cultivating Panax notoginseng adventitious roots, characterized in that: The culture medium is an adventitious root culture medium for callus, which is composed of 1 / 2 MS agar culture medium, 2.0-4.0 mg / L IBA, 0.05-0.15 mg / L KT and 1-8 Vol.% exosomes of giant fungus grass roots; or the culture medium is an adventitious root culture medium for explants, which is composed of MS agar culture medium, 2.0-4.0 mg / L IBA, 0.05-0.15 mg / L KT and 1-8 Vol.% exosomes of giant fungus grass roots; The exosome liquid of giant fungus grass root is obtained by dispersing the exosomes of giant fungus grass root in magnetized water, and the content of the exosomes of giant fungus grass root in the exosome liquid of giant fungus grass root is 20-120 mg / L; The method for preparing exosomes in the exosome fluid of giant fungus grass roots comprises the following steps: Step I: clean the roots of giant fungus grass, crush them and grind them into slurry, and obtain juice after solid-liquid separation; Step II: subjecting the juice to differential centrifugation to obtain exosomes from the roots of giant fungus grass.

2. The use according to claim 1, characterized in that: The differential centrifugation in step II comprises the following steps: centrifuging the juice at a speed of 2500-3500 rpm for 15-25 min; dispersing the precipitate with water, and then centrifuging at a speed of 9000-11000 rpm for 30-50 min; dispersing the precipitate with water, and finally centrifuging at a speed of 14000-16000 rpm for 100-130 min.

3. The use according to claim 1, characterized in that: The preparation method of the magnetized water comprises the following steps: magnetizing water at a magnetic field strength of 0.5-1.5 T for 60-150 minutes to obtain the magnetized water.

4. A method for rapid cultivation of adventitious roots of Panax notoginseng, characterized in that: The method comprises the steps of placing the Panax notoginseng material on the culture medium for rapid culture of Panax notoginseng adventitious roots as described in any one of claims 1 to 3 to induce rooting; When the Panax notoginseng material is Panax notoginseng callus, the method comprises the step of placing the Panax notoginseng callus on an adventitious root culture medium for the callus to induce rooting; Alternatively, when the Panax notoginseng material is a Panax notoginseng explant, the method includes the step of placing the Panax notoginseng explant on an adventitious root culture medium for the explant to induce rooting.

5. The method for rapid cultivation of adventitious roots of Panax notoginseng according to claim 4, characterized in that: When the Panax notoginseng callus tissue is used as the Panax notoginseng material, the culture conditions include: dark culture at 22-28°C for 20-30 days; when the Panax notoginseng explant is used as the Panax notoginseng material, the culture conditions include: dark culture at 22-28°C for 25-70 days.

6. The method for rapid cultivation of adventitious roots of Panax notoginseng according to claim 5, characterized in that: The Panax notoginseng explants are selected from any one or more of Panax notoginseng leaves, Panax notoginseng roots, Panax notoginseng young leaves, Panax notoginseng stem segments, roots of Panax notoginseng callus-induced regenerated seedlings, rooted stem segments of Panax notoginseng callus-induced regenerated seedlings, stem segments of Panax notoginseng callus-induced regenerated seedlings, and young leaves of Panax notoginseng callus-induced regenerated seedlings.

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