Preparation method of anti-skin photoaging composition

Abstract

The present invention discloses a method for preparing an anti-skin photoaging composition, relating to the technical field of skin care products. The present invention uses aspalathus linearis extract, rhodiola rosea extract, and ginkgo biloba extract as main ingredients, and adds edelweiss extract and a silk fibroin nanoparticle solution to prepare the composition. The composition comprises silk fibroin nanoparticles, which have film-forming properties and can be used as a wrapping material to wrap the composition, thereby ensuring the stability of the flavonoid components. The silk fibroin nanoparticles can also be evenly spread on the skin surface and have a good sun protection effect, thereby improving the product's sun protection factor (SPF). Simultaneously, the plant composition and silk fibroin nanoparticles are applied to a sunscreen product with anti-skin photoaging efficacy, thereby scavenging oxidative free radicals caused by ultraviolet rays in cells and improving skin damage, erythema, and cell sunburn caused by ultraviolet radiation. Furthermore, because the composition is made of natural plant ingredients, it has low skin irritation, strong product stability, and significantly improves the SPF.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, in particular to a method for preparing an anti-skin photoaging composition. Background Art

[0002] Skin aging is the result of a combination of endogenous and exogenous factors, with ultraviolet light being one of the primary exogenous factors contributing to skin aging. Ultraviolet light can be categorized by wavelength as UVA (320-400nm), UVB (290-320nm), and UVC (200-290nm). UVC and a portion of UVB are absorbed by the ozone layer. Therefore, UVA and UVB are the primary causes of skin photoaging.

[0003] Ultraviolet radiation stimulates keratinocytes and fibroblasts, inhibiting procollagen synthesis and promoting degradation of the dermal extracellular matrix, leading to photoaging of the skin. The main manifestations of photoaging are decreased dermal elasticity, epidermal thickening, and vasodilation.

[0004] As consumers' aesthetic awareness grows and their demand for multifunctional and natural skincare products increases, sunscreens that simply pursue a high SPF (Sun Protection Factor) are no longer sufficient to meet the needs of consumers seeking to combat photoaging. Given the aforementioned mechanisms of UV-induced photoaging, combating photoaging requires not only protecting against UV damage but also preventing photoaging from within the skin.

[0005] Currently, Chinese patent publication number CN111700817A discloses a composition for resisting skin photoaging and its application, comprising (0.1 to 100) parts by weight of component A and (1000 to 20000) parts by weight of component B, wherein the component A comprises carnosic acid, a pharmaceutically acceptable salt thereof, or at least one solvate thereof, and the component B comprises protopanaxadiol, a pharmaceutically acceptable salt thereof, or at least one solvate thereof.

[0006] This composition acts on different antioxidant channels and sites through carnosic acid and protopanaxadiol, achieving better anti-photoaging effects through synergistic effects. However, the effect of this composition only acts in the antioxidant direction, and the repair effect on skin photoaging caused by ultraviolet rays is not obvious. The raw materials used are highly allergenic and can easily irritate the skin, causing secondary damage to the skin. Summary of the Invention

[0007] The present invention aims to solve the above technical problems, overcome the shortcomings of the prior art, and provide a method for preparing an anti-photoaging composition.

[0008] In order to solve the above technical problems, the present invention provides a method for preparing an anti-skin photoaging composition.

[0009] Technical effect: The present invention uses linear aspalathus extract, rhodiola rosea extract and ginkgo extract as main ingredients, and adds alpine edelweiss extract and silk nanoprotein solution to prepare a composition. The composition is silk protein nanoparticles. Silk fibroin nanoprotein has film-forming properties and can be used as a wrapping material to wrap the composition to ensure the stability of flavonoid components; it can also be evenly spread on the skin surface and has a better sun protection effect, thereby improving the sun protection index of the product; at the same time, the plant composition and silk protein nanoparticles are applied to sunscreen products with anti-skin photoaging effects, which can clear oxidative free radicals caused by ultraviolet rays in cells and improve skin damage, erythema and cell sunburn caused by ultraviolet radiation; at the same time, because the composition is a natural plant ingredient, it has little irritation to the skin, strong product stability, and significantly improves the sun protection index.

[0010] The technical solution further defined in the present invention is: a method for preparing an anti-skin photoaging composition, comprising the following components by weight:

[0011]

[0012] The balance is deionized water;

[0013] Among them, the content ratio between the linear aspalathus extract, rhodiola rosea extract and ginkgo extract is 12:7:5~17:6:4; the content ratio between the alpine edelweiss extract and the linear aspalathus extract is 1:9~1:5.

[0014] Furthermore, the linear aspalathus extract, the alpine edelweiss extract, the rhodiola rosea extract and the ginkgo biloba extract are mixed into a plant composition, and the plant composition exists in the form of silk protein nanoparticles;

[0015] Phase A material includes lecithin, C16-18 alcohol, antioxidant and chelating agent, and linear aspalathus extract, rhodiola rosea extract and ginkgo extract are dissolved in the phase A material;

[0016] Phase B material includes GTCC, cholesterol and ceramide E.

[0017] In the aforementioned method for preparing an anti-skin photoaging composition, silk protein nanoparticles are prepared by the following steps:

[0018] S1, taking a certain amount of linear aspalathus extract, alpine edelweiss extract, rhodiola rosea extract and ginkgo extract and mixing them into a plant composition, adding the plant composition into 75% ethanol solution, and heating at 40℃.

[0019] stirring under heating in a water bath for 30 minutes to dissolve the plant composition in ethanol to obtain a plant composition ethanol solution;

[0020] S2, taking a quantitative 0.1 g / L concentration of silk fibroin solution, mixing the plant combination ethanol solution and the silk fibroin solution evenly, and then removing the ethanol by rotary evaporation to obtain a plant combination-silk protein nanosuspension;

[0021] S3, taking a certain amount of lecithin, C16-18 alcohol, antioxidant and chelating agent, adding them to 75% ethanol solution, and heating them in a water bath to 40°C while stirring to form phase A;

[0022] S4, taking a certain amount of GTCC, cholesterol and ceramide E, dissolving them in 75% ethanol solution in a 75°C water bath to obtain phase B material;

[0023] S5. Add phase B material to phase A material, fully dissolve it, remove ethanol by rotary evaporation, and evenly mix it with the plant combination-silk protein nano suspension to obtain a mixed solution. Add the mixed solution to a homogenizer and homogenize it for 5 minutes to fully emulsify it, and cool it to room temperature to obtain silk protein nanoparticles.

[0024] The above-mentioned method for preparing an anti-skin photoaging composition, wherein the linear aspalathus extract is prepared by the following steps:

[0025] S1, taking linear aspalathus leaves after quantitative fermentation, repeatedly washing them with clean water to remove dirt and attachments, then washing with distilled water and drying them, and freezing them at -18°C to -25°C for 8 hours; taking out the frozen linear aspalathus leaves, grinding them in a mortar and setting aside;

[0026] S2, adding the ground aspalathus linear leaves to 75% ethanol, ultrasonically extracting for 20 min, filtering to obtain a crude extract, and adding deionized water to rinse until turbid to obtain a suspension;

[0027] S3. Add a macroporous resin to the suspension and adsorb for 30 to 60 minutes. Remove the macroporous resin and elute with 95% ethanol to obtain an eluate. Vacuum concentrate the eluate to dryness and add a certain amount of deionized water to adjust the eluate to a specified concentration to obtain a linear aspalathus extract.

[0028] The aforementioned method for preparing an anti-skin photoaging composition, wherein the Rhodiola rosea extract is prepared by the following steps:

[0029] S1, taking a certain amount of Rhodiola rosea, adding deionized water, decocting for 2-4 hours, controlling the decoction temperature at 93-98°C, filtering to obtain a decoction, concentrating, and adding 75% ethanol to precipitate to obtain a precipitate;

[0030] S2, drying and crushing the decocted Rhodiola rosea solid to obtain a crushed product, adding deionized water and ultrasonically extracting for 30 minutes, and filtering to obtain an extract;

[0031] S3, mixing the extract and the precipitate, concentrating under reduced pressure, and adding a certain amount of deionized water to obtain a Rhodiola rosea extract.

[0032] In the aforementioned method for preparing an anti-photoaging composition, the ginkgo extract is prepared by the following steps:

[0033] S1: adding 75% ethanol to the ginkgo leaves to extract the ginkgo leaves for more than 4 times at a temperature of 75-85°C, then collecting the extract, vacuum evaporating, recovering the ethanol, and centrifuging to obtain the supernatant;

[0034] S2, adding macroporous resin to the supernatant for adsorption, eluting with 70% ethanol until the liquid is clear, then desorbing with 95% ethanol, collecting the desorbed liquid, recovering the ethanol under reduced pressure, adding deionized water to prepare to a specified concentration, and obtaining the ginkgo leaf extract.

[0035] In the aforementioned method for preparing an anti-skin photoaging composition, the antioxidant is β-carotene, anthocyanin, or grape seed extract.

[0036] In the above-mentioned method for preparing an anti-skin photoaging composition, the chelating agent is disodium EDTA, polyphosphate or aminocarboxylic acid.

[0037] In the aforementioned method for preparing an anti-skin photoaging composition, the optimal content ratio between the linear aspalathus extract, the rhodiola rosea extract and the ginkgo biloba extract is 14:6:5.

[0038] In the aforementioned method for preparing the anti-skin photoaging composition, the optimal content ratio between the alpine edelweiss extract and the linear aspalathus extract is 1:7.

[0039] The beneficial effects of the present invention are:

[0040] (1) In the present invention, the main active ingredients are aspalathus linearis extract, rhodiola rosea extract, ginkgo extract, and leontopodium alpinum extract. Aspalathus linearis extract contains phenolic and flavonoid compounds, which give it good antioxidant activity, effectively scavenging oxidative free radicals caused by ultraviolet rays and preventing oxidative damage to the skin;

[0041] (2) In the present invention, salidroside is the main active ingredient of Rhodiola rosea, has excellent antioxidant effect and inhibits tyrosinase activity, and has a protective effect on UVB-induced oxidative damage and cell apoptosis. It also reduces UV-induced skin inflammation by targeting cyclooxygenase 2 (COX-2) and is non-toxic to cells. Therefore, after being added to the composition, the composition can have good anti-inflammatory properties;

[0042] (3) In the present invention, the ginkgo extract is rich in flavonoids such as rutin and quercetin. Rutin and quercetin have maximum absorption peaks at 373 and 341 nm, respectively. Ginkgo extract has UVA photoprotective effects. It can improve barrier damage, erythema, and sunburned cells caused by ultraviolet radiation. At the same time, salidroside extract and ginkgo extract both have strong free radical scavenging activity and are widely used in natural products with antioxidant effects.

[0043] (4) In the present invention, under the action of the alpine edelweiss extract, the Rhodiola rosea extract, the Ginkgo biloba extract, and the linear aspalathus extract can produce a synergistic effect, enhancing the antioxidant activity of the composition and improving the effect of clearing oxidative free radicals. At the same time, it can repair sunburned cells and the skin barrier, greatly improving the sun protection and skin repair effects of the composition. In addition, the addition of the alpine edelweiss extract can also inhibit the reaction of ions in the chelating agent in the skin, reduce skin irritation, and activate the intracellular antioxidant defense system;

[0044] (5) In the present invention, a composition is prepared by using linear aspalathus extract, rhodiola rosea extract and ginkgo extract as main ingredients, adding alpine edelweiss extract and silk nanoprotein solution, and the composition is silk protein nanoparticles. Silk nanoprotein has film-forming properties and can be used as a wrapping material to wrap the composition to ensure the stability of the flavonoid components; it can also be evenly spread on the skin surface and has a better sun protection effect, thereby improving the sun protection index of the product; at the same time, the plant composition and silk protein nanoparticles are applied to sunscreen products with anti-skin photoaging effects, which can clear oxidative free radicals caused by ultraviolet rays in cells and improve skin damage, erythema and cell sunburn caused by ultraviolet radiation; at the same time, because the composition is a natural plant ingredient, it has little irritation to the skin, has strong product stability, and significantly improves the sun protection index. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 The figure is a comparison chart of the ultraviolet absorption effect of the best embodiment and the comparative example of the present invention. DETAILED DESCRIPTION

[0046] This embodiment provides a method for preparing an anti-photoaging composition for skin, comprising the following components by weight:

[0047]

[0048]

[0049] The balance was deionized water.

[0050] Among them, the content ratio between linear aspalathus extract, rhodiola rosea extract and ginkgo extract is 12:7:5~17:6:4, and the optimal content ratio is 14:6:5; the content ratio between alpine edelweiss extract and linear aspalathus extract is 1:9~1:5, and the optimal content ratio is 1:7.

[0051] In the present invention, the plant composition is prepared in the form of silk protein nanoparticles, which can be easily applied by users and has the effect of transdermal delivery. It can increase the retention time and absorption effect of the composition on the skin, making the sun protection time longer, which can reach more than 8 hours.

[0052] The main active ingredients of the invention are aspalathus linearis extract, rhodiola rosea extract, and ginkgo biloba extract. Aspalathus linearis extract contains phenolic and flavonoid compounds, which give it excellent antioxidant activity, effectively scavenging oxidative free radicals caused by ultraviolet rays and preventing oxidative damage to the skin.

[0053] Salidroside is the main active ingredient of Rhodiola rosea, has excellent antioxidant effects and inhibits tyrosinase activity. It also has a protective effect on UVB-induced oxidative damage and cell apoptosis. It also reduces UV-induced skin inflammation by targeting cyclooxygenase 2 (COX-2) and is non-toxic to cells. Therefore, after being added to the composition, the composition can have good anti-inflammatory properties.

[0054] Ginkgo extract is rich in flavonoids such as rutin and quercetin, with maximum absorption peaks at 373 and 341 nm, respectively. Ginkgo extract has UVA photoprotective properties, improving barrier damage, erythema, and sunburn caused by UV radiation. Both salidroside extract and ginkgo extract have strong free radical scavenging activity and are widely used in natural products with antioxidant properties.

[0055] Most importantly, the addition of Leontopodium alpinum extract creates a synergistic effect between Rhodiola rosea extract, Ginkgo biloba extract, and Aspalathus linearis extract, enhancing the composition's antioxidant activity and free radical scavenging capabilities while repairing sunburned cells and the skin barrier, significantly improving the composition's sun protection and skin repair effects. Furthermore, the addition of Leontopodium alpinum extract inhibits the reaction of chelating agent ions within the skin, reducing skin irritation and activating the intracellular antioxidant defense system.

[0056] Alpine edelweiss extract contains compounds such as mysabolane derivatives, chlorogenic acid, β-sitosterol and luteolin, which can initiate the skin's natural healing process, protect the skin from environmental pollution and other external factors, stimulate skin cell production, fade wrinkles, and prevent premature skin aging. It also has extraordinary antioxidant, anti-inflammatory and anti-free radical properties, protect skin collagen, and reduce oxidative stress on the skin.

[0057] After adding the alpine edelweiss extract, the composition also has good skin permeability and skin affinity, allowing the composition to enter the basal layer of the skin through the skin water channels, thereby significantly improving the sun protection effect and anti-inflammatory and anti-aging properties of the composition.

[0058] The phase A material of the present invention comprises lecithin, C16-18 alcohol, antioxidant and chelating agent, and the linear aspalathus extract, rhodiola rosea extract and ginkgo extract are dissolved in the phase A material;

[0059] Phase B material includes GTCC, cholesterol and ceramide E.

[0060] The antioxidant used in the present invention is β-carotene, anthocyanin or grape seed extract, all of which have good antioxidant effects, can give silk protein nanoparticles stronger antioxidant properties, increase the shelf life of the composition, and further enhance the antioxidant effect of the composition on the skin.

[0061] The chelating agent used in the present invention is disodium EDTA, polyphosphate or aminocarboxylic acid, which can play a synergistic role in the antiseptic system and antioxidant system of the composition, thereby improving the shelf life of the composition.

[0062] In the present invention, the linear aspalathus extract is prepared by the following steps:

[0063] S1, taking linear aspalathus leaves after quantitative fermentation, repeatedly washing them with clean water to remove dirt and attachments, then washing with distilled water and drying them, and freezing them at -18°C to -25°C for 8 hours; taking out the frozen linear aspalathus leaves, grinding them in a mortar and setting aside;

[0064] S2, adding the ground aspalathus linear leaves to 75% ethanol, ultrasonically extracting for 20 min, filtering to obtain a crude extract, and adding deionized water to rinse until turbid to obtain a suspension;

[0065] S3. Add a macroporous resin to the suspension and adsorb for 30 to 60 minutes. Remove the macroporous resin and elute with 95% ethanol to obtain an eluate. Vacuum concentrate the eluate to dryness and add a certain amount of deionized water to adjust the eluate to a specified concentration to obtain a linear aspalathus extract.

[0066] Freezing and then grinding the fermented aspalathus linear leaves can preserve the active ingredients in the leaves during the grinding process, avoiding their destruction. The optimal freezing condition is -22°C. Temperatures below -25°C easily destroy the ingredients in the leaves, while temperatures above -18°C make it difficult to achieve the desired freezing effect. Furthermore, the present invention utilizes a combination of ultrasonic extraction and macroporous resin extraction to extract the leaves, achieving an effective ingredient extraction rate exceeding 98%, significantly improving preparation efficiency.

[0067] Rhodiola rosea extract is prepared by the following steps:

[0068] S1, taking a certain amount of Rhodiola rosea, adding deionized water, decocting for 2-4 hours, controlling the decoction temperature at 93-98°C, filtering to obtain a decoction, concentrating, and adding 75% ethanol to precipitate to obtain a precipitate;

[0069] S2, drying and crushing the decocted Rhodiola rosea solid to obtain a crushed product, adding deionized water and ultrasonically extracting for 30 minutes, and filtering to obtain an extract;

[0070] S3, mixing the extract and the precipitate, concentrating under reduced pressure, and adding a certain amount of deionized water to obtain a Rhodiola rosea extract.

[0071] The present invention controls the decoction temperature by boiling in water, and the optimal decoction temperature is 96°C, which can avoid destroying the effective ingredients in Rhodiola rosea. Ethanol precipitation is added after decoction, which is conducive to the precipitation of the effective ingredients. Finally, the effective ingredients in the decocted solid matter are extracted through ultrasonic extraction, which further improves the extraction rate of the effective ingredients in Rhodiola rosea and increases the extraction rate to more than 99%, with excellent effect.

[0072] Ginkgo extract is prepared by the following steps:

[0073] S1: adding 75% ethanol to the ginkgo leaves to extract the ginkgo leaves for more than 4 times at a temperature of 75-85°C, then collecting the extract, vacuum evaporating, recovering the ethanol, and centrifuging to obtain the supernatant;

[0074] S2, adding macroporous resin to the supernatant for adsorption, eluting with 70% ethanol until the liquid is clear, then desorbing with 95% ethanol, collecting the desorbed liquid, recovering the ethanol under reduced pressure, adding deionized water to prepare to a specified concentration, and obtaining the ginkgo leaf extract.

[0075] The invention adopts multiple extractions and multi-stage centrifugation to effectively and completely extract the effective components of the ginkgo leaf raw material and improve the extraction effect of the ginkgo leaf extract.

[0076] Aspalathus linearis extract, Leontopodium alpinum extract, Rhodiola rosea extract, and Ginkgo biloba extract are mixed to form a botanical composition, which is in the form of silk protein nanoparticles. The silk protein nanoparticles are prepared by the following steps:

[0077] S1, taking a certain amount of linear aspalathus extract, alpine edelweiss extract, rhodiola rosea extract and ginkgo extract and mixing them to form a plant composition, adding the plant composition to a 75% ethanol solution, and stirring in a 40°C water bath for 30 minutes to dissolve the plant composition in the ethanol, thereby obtaining a plant composition ethanol solution;

[0078] S2, taking a quantitative 0.1 g / L concentration of silk fibroin solution, mixing the plant combination ethanol solution and the silk fibroin solution evenly, and then removing the ethanol by rotary evaporation to obtain a plant combination-silk protein nanosuspension;

[0079] S3, taking a certain amount of lecithin, C16-18 alcohol, antioxidant and chelating agent, adding them to 75% ethanol solution, and heating them in a water bath to 40°C while stirring to form phase A;

[0080] S4, taking a certain amount of GTCC, cholesterol and ceramide E, dissolving them in 75% ethanol solution in a 75°C water bath to obtain phase B material;

[0081] S5. Add phase B material to phase A material, fully dissolve it, remove ethanol by rotary evaporation, and evenly mix it with the plant combination-silk protein nano suspension to obtain a mixed solution. Add the mixed solution to a homogenizer and homogenize it for 5 minutes to fully emulsify it, and cool it to room temperature to obtain silk protein nanoparticles.

[0082] The present invention also verifies the antioxidant effect, ultraviolet absorption effect and skin barrier repair effect of the composition on the skin through several examples, and at the same time verifies the synergistic effect of linear aspalathus extract and alpine edelweiss extract on the antioxidant effect and sun protection effect of the composition through an orthogonal method.

[0083] The present invention adopts the "Determination Method of Long-wave Ultraviolet Protection Index PFA of Sunscreen Cosmetics" in the "Cosmetic Safety Technical Specification" (2015 edition) to determine the protection index PFA value of the composition, and refers to the QB / T1857-2013 cream stability inspection standard; the test is conducted on a group of people aged 22 to 35 years old, with 10 males and 10 females tested, and the values are averaged;

[0084] The SPF value was also measured using the "Determination Method for Waterproof Performance of Sunscreen Cosmetics" in the "Technical Specifications for Safety of Cosmetics" with a measurement time of 80 minutes, and reference was made to the QB / T1857-2013 inspection standard for cream stability; people aged between 22 and 35 were selected for the measurement, with 10 males and 10 females, and the values were averaged.

[0085] In addition, the present invention also provides a comparative example, in which common sunscreens on the market are purchased and the sun protection effect of the composition of the present invention is compared, and the SPF value and PFA value are also measured by the above method.

[0086] The present invention sets the linear aspalathus extract experimental number as A X ; The number of Rhodiola rosea extracts is set to B Y ; Ginkgo extract content is set to C Z ; The number of parts of alpine edelweiss extract is set to D M：N , X:Y:Z is the content ratio of the linear aspalathus extract, the Rhodiola rosea extract, and the Ginkgo biloba extract, and M:N is the content ratio of the linear aspalathus leaf extract and the alpine edelweiss extract. The results are shown in Table 1.

[0087] Table 1 SPF and PFA values measured in different experiments

[0088]

[0089]

[0090] Referring to Table 1, in experiments with test numbers S1-S3, linear aspalathus extract, rhodiola rosea extract and ginkgo biloba extract were used in the composition. The three acted independently, and the sun protection effect of the composition was limited, with the highest SPF value being 20.1 and the highest PFA value being 10.4; in experiments S4-S6, the effects of the combination of linear aspalathus extract, rhodiola rosea extract and ginkgo biloba extract were verified, among which the linear aspalathus extract had the greatest impact on the sun protection effect of the composition; in addition, when combined in pairs, the sun protection effect of the composition was poor.

[0091] In tests S7-S9, alpine edelweiss extract was added in pairs. Test S9 shows that adding alpine edelweiss extract to Rhodiola rosea extract and Ginkgo biloba extract has limited effect on the sunscreen effect of the composition. Tests S7 and S8 show that the simultaneous presence of linear aspalathus extract and alpine edelweiss extract significantly enhances the sunscreen effect of the composition. This preliminarily suggests a synergistic effect between the linear aspalathus extract and the alpine edelweiss extract, enhancing the sunscreen effect of the composition.

[0092] It can be seen from the experiments of S10-S12 that after adding alpine edelweiss extract to linear aspalathus extract, rhodiola rosea extract and ginkgo extract, the sun protection performance of the composition can be greatly improved. The linear aspalathus extract and alpine edelweiss extract have an excellent synergistic effect on rhodiola rosea extract and ginkgo extract, which greatly improves the sun protection performance of the composition. At the same time, the composition in S11 has the best sun protection effect, with an average SPF value of 48.6 and an average PFA value of 17.4, which are much stronger than similar sunscreens on the market - that is, the sun protection effect in the comparative example.

[0093] It can be seen that when the content ratio of linear aspalathus extract, Rhodiola rosea extract and Ginkgo biloba extract is 14:6:5; when the content ratio of alpine edelweiss extract and linear aspalathus extract is 1:7, the prepared composition has the best sun protection effect.

[0094] The present invention uses the optimal content of each component to prepare the embodiment, and compares it with the comparative example formed by commercial sunscreen to obtain a comparison chart of ultraviolet absorption effect. The results are as follows Figure 1 As shown, it can be seen that the ultraviolet absorbance of the best embodiment of the present invention is much higher than that of ordinary sunscreens on the market, with the highest absorbance reaching 4, and the ultraviolet absorption effect is excellent.

[0095] In addition, to verify the role of the plant composition in the sunscreen composition, the present invention also conducted different control experiments. Experimental group A was a sunscreen formed by adding the plant composition to the ratio of experimental group S11, and experimental group B was a sunscreen formed without adding the plant composition. The SPF value and PFA value were also measured using the above method. The results are shown in Table 2.

[0096] Table 2 SPF and PFA values measured in the control experimental group

[0097] Experimental group SPF value PFA value A 48.6 17.4 B 6.2 3.3

[0098] As can be seen from Table 2, after adding the plant composition to the sunscreen, the sun protection effect of the finished sunscreen is greatly improved, and its SPF value and PFA value are significantly improved, which proves the superiority of the sun protection effect of the plant composition in the present invention.

[0099] In addition to the above embodiments, the present invention may also have other implementations. Any technical solution formed by equivalent replacement or equivalent transformation falls within the scope of protection required by the present invention.

Claims

1. A method for preparing an anti-skin photoaging composition, characterized in that: Materials containing the following components by weight: 36-41 parts of linear aspalathus extract 18-21 parts of Rhodiola rosea extract 12-15 parts of Ginkgo extract 4-8 parts of alpine edelweiss extract 14-22 parts of phase A 12-18 parts of Phase B 22-28 parts of silk fibroin solution The balance is deionized water; The content ratio of the linear aspalathus extract, the rhodiola rosea extract and the ginkgo biloba extract is 12:7:5 to 17:6:4; the content ratio of the alpine edelweiss extract to the linear aspalathus extract is 1:9 to 1:5; The phase A material includes lecithin, C16-18 alcohol, antioxidant and chelating agent; The phase B material includes GTCC, cholesterol and ceramide E.

2. The method for preparing an anti-skin photoaging composition according to claim 1, wherein: The linear aspalathus extract, alpine edelweiss extract, rhodiola rosea extract and ginkgo biloba extract are mixed to form a plant composition, and the plant composition exists in the form of silk protein nanoparticles; The linear aspalathus extract, rhodiola rosea extract and ginkgo biloba extract are dissolved in the phase A material.

3. The method for preparing an anti-skin photoaging composition according to claim 2, wherein: The silk protein nanoparticles are prepared by the following steps: S1, taking a certain amount of linear aspalathus extract, alpine edelweiss extract, rhodiola rosea extract and ginkgo biloba extract and mixing them to form a plant composition, adding the plant composition to a 75% ethanol solution, and stirring in a 40°C water bath for 30 minutes to dissolve the plant composition in ethanol, thereby obtaining a plant composition ethanol solution; S2, taking a quantitative 0.1 g / L concentration of silk fibroin solution, mixing the plant combination ethanol solution and the silk fibroin solution evenly, and then removing the ethanol by rotary evaporation to obtain a plant combination-silk protein nanosuspension; S3, taking a certain amount of lecithin, C16-18 alcohol, antioxidant and chelating agent, adding them to 75% ethanol solution, and heating them in a water bath to 40°C while stirring to form phase A; S4, taking a certain amount of GTCC, cholesterol and ceramide E, dissolving them in 75% ethanol solution in a 75°C water bath to obtain phase B material; S5. Add phase B material to phase A material, fully dissolve it, remove ethanol by rotary evaporation, and evenly mix it with the plant combination-silk protein nano suspension to obtain a mixed solution. Add the mixed solution to a homogenizer and homogenize it for 5 minutes to fully emulsify it, and cool it to room temperature to obtain silk protein nanoparticles.

4. The method for preparing an anti-skin photoaging composition according to claim 1, wherein: The linear aspalathus extract is prepared by the following steps: S1, taking linear aspalathus leaves after quantitative fermentation, repeatedly washing them with clean water to remove dirt and attachments, then washing with distilled water and drying them, and freezing them at -18°C to -25°C for 8 hours; taking out the frozen linear aspalathus leaves, grinding them in a mortar and setting aside; S2, adding the ground aspalathus linear leaves to 75% ethanol, ultrasonically extracting for 20 min, filtering to obtain a crude extract, and adding deionized water to rinse until turbid to obtain a suspension; S3. Add macroporous resin to the suspension and adsorb for 30 to 60 minutes. Remove the macroporous resin and elute with 95% ethanol to obtain an eluate. Vacuum concentrate the eluate to dryness and add a certain amount of deionized water to adjust the concentration to a specified concentration to obtain a linear aspalathus extract.

5. The method for preparing an anti-skin photoaging composition according to claim 1, characterized in that: The Rhodiola rosea extract is prepared by the following steps: S1, taking a certain amount of Rhodiola rosea, adding deionized water, decocting for 2-4 hours at a temperature of 93-98°C, filtering to obtain a decoction, concentrating, and adding 75% ethanol to obtain a precipitate; S2, drying and crushing the decocted Rhodiola rosea solid to obtain a crushed product, adding deionized water and ultrasonically extracting for 30 minutes, and filtering to obtain an extract; S3, mixing the extract and the precipitate, concentrating under reduced pressure, and adding a certain amount of deionized water to obtain a Rhodiola rosea extract.

6. The method for preparing an anti-skin photoaging composition according to claim 1, characterized in that: The ginkgo extract is prepared by the following steps: S1: adding 75% ethanol to the ginkgo leaves to extract the ginkgo leaves for more than 4 times at a temperature of 75-85°C, then collecting the extract, vacuum evaporating, recovering the ethanol, and centrifuging to obtain the supernatant; S2. Add macroporous resin to the supernatant for adsorption, elute with 70% ethanol until the liquid is clear, then desorb with 95% ethanol, collect the desorbed liquid, recover the ethanol under reduced pressure, add deionized water to prepare to a specified concentration, and obtain the ginkgo leaf extract.

7. The method for preparing an anti-skin photoaging composition according to claim 1 or 3, characterized in that: The antioxidant is beta-carotene, anthocyanin or grape seed extract.

8. The method for preparing an anti-skin photoaging composition according to claim 1 or 3, characterized in that: The chelating agent is disodium EDTA, polyphosphate or aminocarboxylic acid.

9. The method for preparing an anti-photoaging composition according to claim 1, wherein: The weight ratio of the linear aspalathus extract, the rhodiola rosea extract and the ginkgo biloba extract is 14:6:

5.

10. The method for preparing an anti-skin photoaging composition according to claim 1, characterized in that: The weight ratio between the alpine edelweiss extract and the linear aspalathus extract is 1:7.

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