Method for manufacturing three-dimensional microreactor and three-dimensional microreactor

Abstract

The application relates to the field of microfluidic technology, in particular to a three-dimensional micro-reactor manufacturing method and a three-dimensional micro-reactor. The manufacturing method comprises the following steps: manufacturing a metal mold; manufacturing a PMMA groove, fixing the two ends of the metal mold on the PMMA groove; adding a mixture into the PMMA groove; solidifying the mixture containing the metal mold to obtain a PDMS solidified product containing the metal mold; exposing the metal mold; making a gap between the metal mold and the PDMS solidified product, and then separating the metal mold from the PDMS solidified product to form a micro-channel; injecting ultraviolet light curing glue into the two end ports of the micro-channel, irradiating the ultraviolet light curing glue in the micro-channel by using an ultraviolet lamp, and sealing the two ends of the micro-channel; setting an inlet and an outlet, connecting a stainless steel capillary to each of the inlet and the outlet, and connecting a long and thin hose, and the manufacturing is completed; the manufacturing method has the advantages of simple process, short manufacturing period, no need of professional equipment and technical personnel in the manufacturing process, reduced manufacturing cost and improved manufacturing efficiency.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, and more particularly to a method for fabricating a three-dimensional microreactor and the three-dimensional microreactor itself. Background Technology

[0002] The development of 3D manufacturing technology has driven the advancement of 3D microfluidics. 3D microfluidics has demonstrated advantages in various processes, including solution mixing, cell culture, drug screening, chemical reactions, cell separation, and sorting. Microreactors with complex 3D structures can not only improve the integration of microfluidic systems but also expand their application functions and enhance detection performance. An increasing number of microreactors with flexible shapes and sizes are expected to improve efficiency and potentially become alternatives to complex 3D systems.

[0003] However, in existing microfabrication technologies, the fabrication and rapid processing of complex three-dimensional microreactors still face significant challenges. Traditional manufacturing methods, such as photolithography, CNC micromachining, and laser ablation, while achieving remarkable progress over decades, still face numerous limitations in producing complex topologies and three-dimensional microchannels. Even with the most complex multilayer mask fabrication, these methods can only produce two-dimensional or simple three-dimensional devices. Their processing equipment is expensive, the manufacturing process is complex, and the yield is low. Furthermore, many microreactor channel structures, such as three-dimensional helical microchannels, are even more difficult to fabricate, becoming a bottleneck in three-dimensional microfluidic fabrication technology research. Therefore, there is an urgent need to develop a new method for fabricating three-dimensional microreactors. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a method for fabricating a three-dimensional microreactor. This method is simple to manufacture, requires no specialized personnel or equipment, is inexpensive, and allows for flexible modification of the shape and size of the microreactor to meet different needs. The fabricated three-dimensional microreactor does not require a cumbersome sealing process and can be reused.

[0005] This invention provides a method for fabricating a three-dimensional microreactor, the method comprising the following steps;

[0006] S1. Design a three-dimensional model of the metal mold and fabricate the metal mold; the metal mold is used to fabricate the microchannels of the three-dimensional microreactor.

[0007] S2. Make a PMMA groove, fix the metal mold on the PMMA groove, so that the bottom of the metal mold is suspended in the PMMA groove;

[0008] A mixture comprising PDMS and a curing agent is added to the PMMA tank, such that the metal mold is completely submerged in the uncured mixture;

[0009] S3. Curing the mixture containing the metal mold to obtain a PDMS cured product containing the metal mold;

[0010] S4. Cut off both ends of the PDMS cured material to expose the metal mold; create a gap between the metal mold and the PDMS cured material, and then detach the metal mold from the PDMS cured material to form a microchannel;

[0011] S5. Inject UV-curable adhesive into both ends of the microchannel, irradiate the UV-curable adhesive in the microchannel with a UV lamp, and seal both ends of the microchannel.

[0012] S6. An inlet and an outlet are provided at adjacent positions at both ends of the sealed microchannel; a stainless steel capillary tube is connected to the inlet and the outlet respectively, and a long thin flexible tube is connected to them, which serve as the microfluidic input tube and the microfluidic output tube respectively, thus completing the fabrication of the three-dimensional microreactor.

[0013] Preferably, after manufacturing the metal mold, the process further includes the following steps:

[0014] S11. Clean the metal mold with anhydrous ethanol;

[0015] S12. Immerse the metal mold in mineral oil and remove the mineral oil from the surface of the metal mold using filter paper.

[0016] Preferably, the three-dimensional model of the metal mold has a spiral structure.

[0017] Preferably, the metal mold is manufactured using three-dimensional manufacturing technology, which is selected from at least one of CNC machine tools, 3D printing, laser cutting, digital modeling, fused deposition modeling, selective laser sintering, photopolymerization, electron beam melting, electron beam processing, solid freeform forming, or nanofabrication.

[0018] Preferably, the height of the PMMA groove is 2mm higher than the height of the metal mold; and the thickness of the bottom PMMA sheet in the PMMA groove is greater than 5mm.

[0019] Preferably, the mixture containing the metal mold is cured under constant temperature conditions; the constant temperature conditions include placing the PMMA bath in a constant temperature chamber, the constant temperature of the constant temperature chamber being 60°C to 80°C, and the constant temperature time of the constant temperature chamber being 3 hours to 6 hours.

[0020] Preferably, creating a gap between the metal mold and the PDMS cured material includes placing the PDMS cured material with both ends exposed above the metal mold into a constant temperature chamber; removing the PDMS cured material; rotating, squeezing, and bending the PDMS cured material and the metal mold to create a gap between the metal mold and the PDMS cured material.

[0021] Preferably, the constant temperature of the constant temperature chamber is 90℃~100℃, and the constant temperature time of the constant temperature chamber is 1 minute~2 minutes.

[0022] Preferably, the amount of UV-curable adhesive injected is enough to fill two adjacent microchannels; the UV irradiation time is 3 to 5 minutes.

[0023] The present invention also provides a three-dimensional microreactor, which is prepared by the above-described method for preparing a three-dimensional microreactor.

[0024] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0025] The fabrication method of the three-dimensional microreactor of the present invention can realize the fabrication of microreactors with complex internal structures. By designing different types of metal molds, microchannels with controllable size and shape can be flexibly fabricated. Compared with the three-dimensional microreactors fabricated by existing microfabrication technologies, the fabrication process of the three-dimensional microreactor using this method is simple, has a short production cycle, and does not require professional equipment and technicians. The fabricated microchannels have low surface roughness and high visibility of the microreactor. The fabricated three-dimensional microreactor does not require a strict sealing procedure to ensure chip performance.

[0026] This invention provides a method for fabricating a three-dimensional microreactor. The method uses relatively few and inexpensive materials, which greatly reduces the fabrication cost of the microreactor. The fabrication method of this invention can significantly improve the fabrication efficiency of three-dimensional microreactors. This method can be used in rudimentary laboratories as an ideal method for fabricating three-dimensional microreactors, which is conducive to expanding the application of three-dimensional microfluidics technology. Attached Figure Description

[0027] Figure 1 This is a flowchart illustrating the implementation steps of the method for fabricating a three-dimensional microreactor in a specific embodiment of the present invention;

[0028] Figure 2 This is a three-dimensional model diagram of the metal mold used to fabricate the microchannel of the three-dimensional microreactor in a specific embodiment of the present invention;

[0029] Figure 3 This is a schematic diagram of a metal mold fixed in a PMMA groove according to a specific embodiment of the present invention;

[0030] Figure 4 This is a schematic diagram showing the positions of the inlet and outlet located at adjacent positions at both ends of the microchannel in a specific embodiment of the present invention;

[0031] Figure 5 This is a schematic diagram of the three-dimensional microreactor fabricated in a specific embodiment of the present invention. Detailed Implementation

[0032] In the following description, embodiments of the invention will be described with reference to the accompanying drawings. In the description below, the same modules are denoted by the same reference numerals. Where the same reference numerals are used, their names and functions are also the same. Therefore, their detailed description will not be repeated.

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not constitute a limitation thereof.

[0034] In specific embodiments of the present invention, such as Figure 1 As shown, a method for fabricating a three-dimensional microreactor is provided, the method comprising the following steps;

[0035] S1. Design a three-dimensional model of the metal mold and fabricate the metal mold; the metal mold is used to fabricate the microchannels of the three-dimensional microreactor; after fabricating the metal mold, the steps further include: S11. Clean the metal mold with anhydrous ethanol; S12. Immerse the metal mold in mineral oil and remove the mineral oil from the surface of the metal mold with filter paper.

[0036] In a specific implementation, a three-dimensional model of a metal mold for fabricating microreactor channels is designed; the metal mold has a spiral structure, so the final three-dimensional microchannels also have a spiral structure; the metal mold is fabricated using three-dimensional manufacturing technology; three-dimensional manufacturing technology can be used for computer numerical control machine tools (Computer Numerical Control Machine Tools). The invention employs CNC (Computer Numerical Control, also known as CNC machine tool), 3D printing technology, laser cutting, digital modeling, fused deposition modeling, selective laser sintering, photopolymerization, electron beam melting, electron beam processing, solid freeform forming, and nanotechnology. In a preferred embodiment, the three-dimensional manufacturing technology used in the method is CNC. After fabricating the metal mold, anhydrous ethanol is added to a first container. The metal mold is then placed in the first container. Next, the first container containing the metal mold and anhydrous ethanol is placed in an ultrasonic cleaner to clean the surface of the metal mold. After cleaning, the surface of the metal mold is dried using nitrogen gas. Mineral oil is added to a second container. The cleaned metal mold is then placed in the second container, completely submerged in the mineral oil. The metal mold is then removed and placed on filter paper to absorb excess mineral oil from its surface. The mineral oil primarily serves a lubricating function; in other embodiments, different types of oil can be used to achieve different lubrication effects as needed.

[0037] S2. Fabricate a PMMA (polymethyl methacrylate) tank, fix the metal mold onto the PMMA tank, and suspend the bottom of the metal mold in the PMMA tank. Specifically, the PMMA tank is provided with bosses, and both ends of the metal mold are fixed to the bosses, thereby suspending the bottom of the metal mold in the PMMA tank. Add a mixture to the PMMA tank, the mixture including PDMS (polydimethylsiloxane) and a curing agent, so that the metal mold is completely submerged in the uncured mixture.

[0038] In a specific implementation, the PMMA groove is sliced ​​along a direction parallel to the bottom surface of the groove to obtain cross-sections A1, A2, A3, A4...An in sequence. CAD drawings of each cross-section structure are then created based on the structural shape. Double-sided adhesive is applied to one or both sides of the PMMA board according to the positional relationship of each cross-section. A laser engraving machine is used to cut the PMMA board with the double-sided adhesive applied to create the cross-section structures of the PMMA groove. Cross-sections A1, A2, A3, A4...An are sequentially bonded together along a direction perpendicular to the bottom surface of the groove to obtain the PMMA groove. The height of the PMMA groove is 2mm higher than the height of the metal mold. The PMMA sheets have different thicknesses; in the PMMA groove, the thickness of the bottom PMMA sheet is >5mm to ensure that the bottom plate does not deform during heating in the constant temperature chamber. The cross-sections bonded to the bottom plate have boss structures to fix both ends of the metal mold to the bosses, ensuring that the bottom of the metal mold is suspended in the PMMA groove. In other implementations, other structural settings can be used to make the bottom of the metal mold suspended in the PMMA groove.

[0039] In a specific implementation, a layer of double-sided tape is adhered to the protrusion of the PMMA tank; then, a metal mold with an oil film formed on its surface is gently placed into the PMMA tank, fixing both ends of the metal mold above the double-sided tape on the protrusion; next, a mixture of PDMS and curing agent is injected into the PMMA tank containing the metal mold, so that the metal mold is completely submerged in the uncured PDMS, i.e., submerged in the uncured mixture; specifically, the mass ratio of PDMS to curing agent is 10:1, and the curing agent can be various commonly used curing agents in the industry, such as SYLGARD purchased from Dow Corning. TM 184Silicone Elastomer CuringAgent, etc.

[0040] S3. Curing the mixture containing the metal mold to obtain a PDMS cured product containing the metal mold; ensuring that the mixture of PDMS and curing agent is cured within a temperature range of 20℃ to 150℃, generally curing speed is faster and curing time is shorter under heating conditions; in specific operation, it is generally placed in an oven for heating and curing.

[0041] In a specific implementation, a PMMA tank containing an uncured PDMS mixture and already placed in a metal mold is placed in a constant temperature chamber to cure the PDMS mixture containing the metal mold, thereby obtaining a cured PDMS product containing the metal mold. The constant temperature chamber is 60℃~80℃, and the constant temperature time is 3 hours~6 hours. When curing the PDMS mixture in a constant temperature chamber, a relatively low constant temperature and a longer constant temperature time can be selected to avoid deformation of the bottom plate of the PMMA tank.

[0042] S4. Cut off both ends of the PDMS cured material to expose the metal mold; create a gap between the metal mold and the PDMS cured material, and then detach the metal mold from the PDMS cured material to form a microchannel.

[0043] In a specific implementation, the PDMS cured material is removed from the constant temperature chamber; then, both ends of the PDMS cured material are cut off to expose the metal mold; then, the PDMS cured material with the metal molds exposed at both ends is placed back into the constant temperature chamber; next, the PDMS cured material with the metal molds exposed is removed, and the PDMS cured material and the metal mold are gently rotated, squeezed, and bent to create a gap between the metal mold and the PDMS cured material; then, any end of the exposed metal mold is selected as the rotation position, and the metal mold is rotated to detach it from the PDMS cured material, ultimately forming a micro-mold. Channels; In a preferred embodiment, the length of the PDMS cured material cut off is the length of 2 to 3 adjacent microchannels, which facilitates clamping the metal mold when unscrewing it; In this step, the constant temperature of the constant temperature chamber is 90℃ to 100℃, and the constant temperature time is 1 minute to 2 minutes. The constant temperature chamber is used to heat the PDMS cured material containing the metal mold. By utilizing the principle of thermal expansion and contraction, the metal mold and the PDMS cured material are more easily separated. Therefore, the constant temperature of the constant temperature chamber does not need to be too high, and the constant temperature time can be slightly shorter, as long as the PDMS cured material containing the metal mold is heated.

[0044] S5. Inject UV-curable adhesive into both ends of the microchannel, irradiate the UV-curable adhesive in the microchannel with a UV lamp, and seal both ends of the microchannel.

[0045] In a specific implementation, 20 mL of anhydrous ethanol is drawn using a syringe; anhydrous ethanol is injected into any port of the microchannel in the three-dimensional microreactor to rinse the microchannel; then, purified water is drawn using a syringe to rinse the microchannel; then, nitrogen gas is used to blow away any remaining water droplets in the microchannel; finally, UV-curable adhesive is injected into both ends of the microchannel, and UV lamps are used to irradiate the UV-curable adhesive in the microchannel to seal both ends of the microchannel; wherein, the UV-curable adhesive serves a sealing function, and in other specific implementations, different types of adhesives can be used to achieve different sealing effects as needed.

[0046] In a specific implementation, the amount of UV-curable adhesive injected is enough to fill two adjacent microchannels, and the UV irradiation time is 3 to 5 minutes. The UV-curable adhesive is used to seal the microchannels. To ensure a tight seal, it is sufficient to fill two adjacent microchannel structures with adhesive. If too much adhesive is injected, the irradiation time should be slightly longer to ensure complete curing of the adhesive.

[0047] S6. An inlet and an outlet are provided at adjacent positions at both ends of the sealed microchannel; a stainless steel capillary tube is connected to the inlet and the outlet respectively, and a long thin flexible tube is connected to them, which serve as the microfluidic input tube and the microfluidic output tube respectively, thus completing the fabrication of the three-dimensional microreactor.

[0048] In a specific implementation, an inlet and an outlet are punched directly above adjacent microchannels at both ends of the sealed microchannel using a punch. A stainless steel capillary tube is connected to each of the inlet and outlet, and a long, thin flexible tube is connected to each, serving as the microfluidic input tube and the microfluidic output tube, respectively. Specifically, one end of the stainless steel capillary tube at the inlet pierces the top PDMS layer and connects to the microchannel, while the other end is connected to a fluid driving device via a long, thin flexible tube. The fluid driving device may include an injection pump, a peristaltic pump, a constant pressure pump, etc. Similarly, one end of the stainless steel capillary tube at the outlet pierces the top PDMS layer and connects to the microchannel, while the other end is connected to a collection tube or a downstream application system via a long, thin flexible tube. This completes the fabrication of the three-dimensional microreactor in this embodiment.

[0049] The present invention also provides a three-dimensional microreactor, which is prepared by the above-described method for preparing a three-dimensional microreactor.

[0050] The fabrication method of the three-dimensional microreactor of the present invention can realize the fabrication of microreactors with complex internal structures. By designing different types of metal molds, microchannels with controllable size and shape can be flexibly fabricated. Compared with the three-dimensional microreactors fabricated by existing microfabrication technologies, the fabrication process of the three-dimensional microreactor using this method is simple, has a short production cycle, and does not require professional equipment and technicians. The fabricated microchannels have low surface roughness and high visibility of the microreactor. The fabricated three-dimensional microreactor does not require a strict sealing procedure to ensure chip performance.

[0051] This invention provides a method for fabricating a three-dimensional microreactor. The method uses relatively few and inexpensive materials, which greatly reduces the fabrication cost of the microreactor. The fabrication method of this invention can significantly improve the fabrication efficiency of three-dimensional microreactors. This method can be used in rudimentary laboratories as an ideal method for fabricating three-dimensional microreactors, which is conducive to expanding the application of three-dimensional microfluidics technology.

[0052] The present invention will now be described in further detail with reference to the embodiments and accompanying drawings.

[0053] Example 1

[0054] 1) Use the commercial computer-aided design software UG to create a 3D model of the metal mold used to fabricate the microchannels of the 3D microreactor. The model is as follows: Figure 2As shown. The metal mold model has a spiral structure, and the final fabricated three-dimensional microchannel also has a spiral structure, which can be used for continuous flow PCR operations, reducing the system's footprint compared to a planar structure;

[0055] 2) Use CNC machining to manufacture metal molds;

[0056] 3) Place the metal mold in a crystallizing dish or beaker, and pour in anhydrous ethanol to completely submerge the metal mold. Place the crystallizing dish or beaker in an ultrasonic cleaner and clean for 10 minutes. After cleaning, use nitrogen gas to dry the surface of the metal mold.

[0057] 4) Place the cleaned metal mold in another crystallizing dish and pour in mineral oil until the liquid level is half the height of the metal mold. Rotate the metal mold to completely coat its surface with mineral oil. Remove the oil-coated metal mold and place it on filter paper to absorb any excess mineral oil from its surface.

[0058] 5) Using commercial computer-aided design software, create two-dimensional planar drawings of each section based on the layered cross-sectional structure of the PMMA groove. Import the drawings into a laser engraving machine and use the machine to cut the PMMA according to the drawings to produce the corresponding structure. The base plate is rectangular with a thickness of 5mm. One layer near the base plate has a boss structure with a thickness of 1mm. The other layers have annular rectangular structures. The PMMA groove is fabricated by bonding the layers sequentially along a direction perpendicular to the bottom of the groove. The PMMA groove is 2mm higher than the metal mold.

[0059] 6) Mix PDMS and curing agent in a 10:1 ratio by weight. Apply a 150-micron thick layer of double-sided tape to the protrusion at the bottom of the PMMA tank. Gently place the metal mold into the PMMA tank, securing both ends to the protrusion, as shown in the diagram. Figure 3 As shown. Slowly pour the mixture into the PMMA tank along the gap between the metal mold and the PMMA tank until the liquid level completely covers the metal mold, but does not exceed the top edge of the PMMA tank.

[0060] 7) Place the PMMA bath in a constant temperature oven, set the temperature to 60℃, and heat for 6 hours until cured. After curing, remove the cured PDMS from the PMMA bath. Cut off both ends of the cured PDMS to expose 2-3 adjacent metal molds. Adjust the temperature of the constant temperature oven to 100℃, place the cured PDMS along with the metal molds into the oven and heat for 2 minutes. Remove the cured material and gently squeeze it, repeating several times. Use needle-nose pliers to clamp the metal molds and gently unscrew them.

[0061] 8) Clean the microchannels repeatedly with anhydrous ethanol and purified water to remove any residual mineral oil. Then, inject UV-curable adhesive into both ends of the microchannels. Irradiate with UV light for 5 minutes to fully cure the adhesive and complete the seal. Use a 0.8mm punch to create the inlet and outlet holes directly above the adjacent microchannels at both ends of the sealed microchannel, as shown below. Figure 4 As shown, a stainless steel capillary tube with an outer diameter of 0.9 mm is connected to both the inlet and outlet. To ensure a stable bond between the stainless steel capillary tubes and the PDMS cured material, adhesive is used to fix the capillary tubes in place. A long, thin flexible tube with an inner diameter of 0.8 mm is connected to the stainless steel capillary tubes, serving as the microfluidic input and microfluidic output tubes, respectively. The three-dimensional microreactor is now complete. Figure 5 As shown, the microchannels fabricated through the embodiments of the present invention have low surface roughness and high visualization of the microreactors; the fabricated three-dimensional microreactors do not require a strict sealing process to ensure chip performance; the fabrication process of the three-dimensional microreactors is simple, has a short production cycle, and does not require specialized equipment and technicians.

[0062] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A method for fabricating a three-dimensional microreactor, characterized in that, The method for fabricating the three-dimensional microreactor includes the following steps; S1. Design a three-dimensional model of the metal mold and fabricate the metal mold; the metal mold is used to fabricate the microchannels of the three-dimensional microreactor; S2. Fabricate a PMMA groove, fix the metal mold onto the PMMA groove, so that the bottom of the metal mold is suspended in the PMMA groove; the height of the PMMA groove is 2mm higher than the height of the metal mold; the thickness of the bottom PMMA sheet in the PMMA groove is greater than 5mm; A mixture comprising PDMS and a curing agent is added to the PMMA tank, such that the metal mold is completely submerged in the uncured mixture; S3. Curing the mixture containing the metal mold to obtain a PDMS cured product containing the metal mold; S4. Cut off both ends of the PDMS cured material to expose the metal mold; A gap is created between the metal mold and the PDMS cured material, and then the metal mold is separated from the PDMS cured material to form a microchannel; Creating a gap between the metal mold and the PDMS cured material includes placing the PDMS cured material with both ends exposed above the metal mold into a constant temperature chamber; removing the PDMS cured material; and rotating, squeezing, and bending the PDMS cured material and the metal mold to create a gap between them. S5. Inject UV-curable adhesive into both ends of the microchannel, irradiate the UV-curable adhesive in the microchannel with a UV lamp, and seal both ends of the microchannel; S6. An inlet and an outlet are provided at adjacent positions at both ends of the sealed microchannel; a stainless steel capillary tube is connected to the inlet and the outlet respectively, and a long thin flexible tube is connected to them, which serve as the microfluidic input tube and the microfluidic output tube respectively, thus completing the fabrication of the three-dimensional microreactor.

2. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The process of creating the metal mold further includes the following steps: S11. Clean the metal mold with anhydrous ethanol; S12. Immerse the metal mold in mineral oil and remove the mineral oil from the surface of the metal mold using filter paper.

3. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The three-dimensional model of the metal mold has a spiral structure.

4. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The metal mold is manufactured using three-dimensional manufacturing technology, which is selected from at least one of CNC machine tools, 3D printing, laser cutting, digital modeling, fused deposition modeling, selective laser sintering, photopolymerization, electron beam melting, electron beam processing, solid freeform forming, or nanofabrication.

5. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The mixture containing the metal mold is cured under constant temperature conditions; the constant temperature conditions include placing the PMMA bath in a constant temperature chamber, the constant temperature of the constant temperature chamber being 60℃~80℃, and the constant temperature time of the constant temperature chamber being 3 hours~6 hours.

6. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The constant temperature chamber has a constant temperature of 90℃~100℃ and a constant temperature time of 1 minute~2 minutes.

7. The method for fabricating a three-dimensional microreactor according to claim 1, characterized in that, The amount of UV-curable adhesive injected is enough to fill two adjacent microchannels; the UV irradiation time is 3 to 5 minutes.

8. A three-dimensional microreactor, characterized in that, The three-dimensional microreactor is prepared by the method for preparing a three-dimensional microreactor according to any one of claims 1 to 7.

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