Veterinary immunity-enhancing antiviral powder and preparation method thereof

Abstract

The present application relates to a kind of veterinary immunity-enhancing antiviral powder and its preparation method, the veterinary immunity-enhancing antiviral powder includes traditional Chinese medicine fermentation powder 60-70%, phoenix tree seed oil 20-30% and dioscorea opuloides mucopolysaccharide 10-20% according to mass percentage;The traditional Chinese medicine fermentation powder is first obtained fermentation product using bacillus velezensis, Candida utilis fermentation scutellaria baicalensis, astragalus, pomegranate peel, hawthorn and polygonatum sibiricum, then is formed by drying, ultrafine grinding to fermentation product.This application can improve the quality concentration of piglet serum immunoglobulin, so that piglet immunity, antiviral capacity is improved, in addition, it has good therapeutic effect on piglet viral gastroenteritis.

Description

Technical Field

[0001] This invention belongs to the field of feed additive technology, specifically relating to a veterinary immune-enhancing and antiviral powder and its preparation method. Background Technology

[0002] After weaning, piglets experience a sudden change in environment and feed due to the interruption of breast milk production, which leads to dysfunction of various systems and a decline in immunity. They are susceptible to various immunosuppressive diseases, such as viral diarrhea, which is characterized by vomiting, diarrhea, and dehydration. Weaned piglets have a high morbidity and mortality rate.

[0003] Currently, the prevention and treatment of immunosuppressive diseases in piglets mainly involves adjusting feed composition (such as highly nutritious and adaptable weaning feed), temperature control, and other environmental controls. Additionally, antibiotics are added to feed to control secondary infections, but this can easily lead to drug resistance, decreased immune function in piglets, drug residues, and environmental pollution. Therefore, improving the piglets' own immunity and antiviral capabilities is an effective way to promote the development of the pig farming industry. Summary of the Invention

[0004] The purpose of this invention is to provide a veterinary immune-enhancing antiviral powder and its preparation method in order to solve the above-mentioned problems.

[0005] The present invention achieves the above objectives through the following technical solutions:

[0006] As a first aspect of the present invention, the present invention provides a veterinary immune-enhancing antiviral powder, comprising, by weight percentage, 60-70% traditional Chinese medicine fermentation powder, 20-30% tung oil, and 10-20% yam polysaccharide;

[0007] The fermented Chinese medicine powder is made by first fermenting Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum using Bacillus belye and Candida utilis to obtain fermented products, and then drying and ultra-fine pulverizing the fermented products.

[0008] As a further optimization of the present invention, the mass ratio of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum is (20-30):(20-30):(5-10):(1-5):(1-5).

[0009] As a further optimization of the present invention, the ratio of the bacterial culture of *Bacillus belye* and *Candida utilis* is 1-3:1.

[0010] As a further optimization of the present invention, the inoculation amount of Bacillus belye and Candida utilis is 3-5% of the total mass of the fermentation material.

[0011] As a second aspect of the present invention, the present invention also provides a method for preparing a veterinary immune-enhancing antiviral powder, comprising the following steps:

[0012] (1) Bacillus belye and Candida utilis were activated and proliferated to 1000 by liquid culture. 9 -10 10 CFU / mL, and then the bacterial solution was inoculated into a mixture of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum, and the water content was controlled at 30-40% for solid-state anaerobic fermentation to obtain the fermentation product;

[0013] (2) The fermented material obtained in step (1) is dried and then subjected to one airflow ultrafine pulverization to obtain Chinese herbal fermented powder;

[0014] (3) Add the evenly mixed tung seed oil and yam polysaccharide to the fermented Chinese medicine powder obtained in step (2), and then pulverize it through secondary airflow to obtain a veterinary immune-enhancing antiviral powder.

[0015] As a further optimization of the present invention, in step (1), the fermentation temperature is 20-30℃ and the fermentation time is 15-20 days.

[0016] As a further optimization of the present invention, the process conditions for the primary airflow ultrafine pulverization are: airflow temperature of 15-20℃, airflow pressure of 7-10 bar, pulverization speed of 8-10 r / min, and pulverization time of 20-25 min; the process conditions for the secondary airflow ultrafine pulverization are: airflow temperature of 10-15℃, airflow pressure of 2-5 bar, pulverization speed of 2-6 r / min, and pulverization time of 10-15 min.

[0017] The beneficial effects of this invention are as follows:

[0018] This invention provides a veterinary immune-enhancing antiviral powder, composed of traditional Chinese medicine fermentation powder, tung seed oil, and yam polysaccharide. The traditional Chinese medicine fermentation powder is obtained by fermenting Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn, and Polygonatum sibiricum with Bacillus belye and Candida utilis. The fermented product is then dried and ultra-finely pulverized. When this antiviral powder is added to the basic diet of piglets, it can increase the serum immunoglobulin concentration of piglets, thereby enhancing their immunity and antiviral ability. In addition, it has a good therapeutic effect on viral gastroenteritis in piglets. Detailed Implementation

[0019] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0020] Example 1

[0021] This embodiment provides a veterinary immune-enhancing and antiviral powder, which, by weight percentage, comprises 60% traditional Chinese medicine fermentation powder, 30% tung oil, and 10% yam polysaccharide;

[0022] Its preparation methods include,

[0023] (1) Bacillus belye and Candida utilis were activated and proliferated to 1000 by liquid culture. 9 -10 10 CFU / mL, then take bacterial solution (Bacillus belye and Candida utilis in a 1:1 ratio) and inoculate it into a mixture of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum at 3% of the total fermentation material mass, control the moisture content at 30%, and carry out solid-state anaerobic fermentation to obtain fermented product. The fermentation temperature is 20℃ and the fermentation time is 20 days. The mass ratio of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum is 20:30:5:5:1.

[0024] (2) The fermented material obtained in step (1) is dried and then subjected to one-time airflow ultra-micro pulverization to obtain Chinese medicine fermentation powder. The process conditions for the one-time airflow ultra-micro pulverization are: airflow temperature of 15℃, airflow pressure of 10 bar, pulverization speed of 10 r / min, and pulverization time of 20 min.

[0025] (3) The fermented Chinese medicine powder obtained in step (2) is mixed with tung seed oil and yam polysaccharide, and then subjected to secondary airflow ultra-micro pulverization to obtain veterinary immune-enhancing antiviral powder. The process conditions for the secondary airflow ultra-micro pulverization are: airflow temperature of 10℃, airflow pressure of 5 bar, pulverization speed of 6 r / min, and pulverization time of 10 min.

[0026] Example 2

[0027] This embodiment provides a veterinary immune-enhancing and antiviral powder, which, by weight percentage, comprises 70% traditional Chinese medicine fermentation powder, 20% tung oil, and 10% yam polysaccharide;

[0028] Its preparation methods include,

[0029] (1) Bacillus belye and Candida utilis were activated and proliferated to 1000 by liquid culture. 9 -10 10CFU / mL, then take bacterial solution (Bacillus belye and Candida utilis in a 2:1 ratio) and inoculate it into a mixture of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum at 5% of the total fermentation material mass, control the moisture content at 30%, and carry out solid-state anaerobic fermentation to obtain fermented product. The fermentation temperature is 30℃ and the fermentation time is 15 days. The mass ratio of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum is 30:20:10:1:5.

[0030] (2) The fermented product obtained in step (1) is dried and then subjected to a single airflow ultra-micro pulverization to obtain Chinese herbal fermentation powder. The process conditions for the single airflow ultra-micro pulverization are: airflow temperature of 20°C, airflow pressure of 7 bar, pulverization speed of 8 r / min, and pulverization time of 25 min.

[0031] (3) The fermented Chinese medicine powder obtained in step (2) is mixed with tung seed oil and yam polysaccharide, and then subjected to secondary airflow ultra-micro pulverization to obtain veterinary immune-enhancing antiviral powder. The process conditions for the secondary airflow ultra-micro pulverization are: airflow temperature of 15℃, airflow pressure of 2 bar, pulverization speed of 2 r / min, and pulverization time of 15 min.

[0032] Example 3

[0033] This embodiment provides a veterinary immune-enhancing and antiviral powder, which, by weight percentage, comprises 60% traditional Chinese medicine fermentation powder, 20% tung oil, and 20% yam polysaccharide;

[0034] Its preparation methods include,

[0035] (1) Bacillus belye and Candida utilis were activated and proliferated to 1000 by liquid culture. 9 -10 10 CFU / mL, then take bacterial solution (Bacillus belye and Candida utilis in a 3:1 ratio) and inoculate it into a mixture of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum at 4% of the total fermentation material mass, control the moisture content at 30%, and carry out solid-state anaerobic fermentation to obtain fermented product. The fermentation temperature is 25℃ and the fermentation time is 12 days. The mass ratio of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum is 25:25:7.5:3:3.

[0036] (2) The fermented product obtained in step (1) is dried and then subjected to one-time airflow ultra-micro pulverization to obtain Chinese medicine fermentation powder. The process conditions for the one-time airflow ultra-micro pulverization are: airflow temperature of 17℃, airflow pressure of 8 bar, pulverization speed of 9 r / min, and pulverization time of 22 min.

[0037] (3) The fermented Chinese medicine powder obtained in step (2) is mixed with tung seed oil and yam polysaccharide, and then subjected to secondary airflow ultra-micro pulverization to obtain veterinary immune-enhancing antiviral powder. The process conditions for the secondary airflow ultra-micro pulverization are: airflow temperature of 13℃, airflow pressure of 3 bar, pulverization speed of 4 r / min, and pulverization time of 12 min.

[0038] Comparative Example 1

[0039] The difference from Example 2 is that this comparative example provides a veterinary immune-enhancing antiviral powder, which, by mass percentage, comprises 70% traditional Chinese medicine fermentation powder, 20% cottonseed oil, and 10% yam polysaccharide, and is prepared using the same method as in Example 2.

[0040] Comparative Example 2

[0041] The difference from Example 2 is that this comparative example provides a veterinary immune-enhancing antiviral powder, which, by mass percentage, comprises 70% traditional Chinese medicine fermentation powder, 20% tung oil, and 10% astragalus polysaccharide, and is prepared using the same method as in Example 2.

[0042] Comparative Example 3

[0043] The difference from Example 2 is that this comparative example provides a veterinary immune-enhancing antiviral powder, which, by mass percentage, consists only of fermented Chinese medicine powder, and the preparation method is the same as in Example 2.

[0044] Comparative Example 4

[0045] The difference from Example 2 is that this comparative example provides a veterinary immune-enhancing antiviral powder, which, by mass percentage, comprises 90% traditional Chinese medicine fermentation powder and 10% yam polysaccharide, and is prepared using the same method as in Example 2.

[0046] Comparative Example 5

[0047] The difference from Example 2 is that this comparative example provides a veterinary immune-enhancing antiviral powder, which, by mass percentage, comprises 80% traditional Chinese medicine fermentation powder and 20% tung oil, and is prepared using the same method as in Example 2.

[0048] One hundred and sixty Duroc × Landrace × Large White three-way crossbred piglets, weaned at 25 days of age, were selected and divided into eight groups according to the principle of equal numbers of males and females and similar body weights. The veterinary immune-enhancing and antiviral powders prepared in Examples 1-3 and Comparative Examples 1-5 were added to the basal diet at a weight percentage of 1%. The experimental period was 28 days. The pigs were housed in raised beds in groups with free access to feed and water. Routine management and immunization programs were conducted according to standard pig farm practices. Serum immunoglobulin (IgG, IgM, IgA) concentrations in the piglets were measured on days 14 and 28 after the start of the experiment. All measurements were performed using a kit method purchased from Nanjing Jiancheng Bioengineering Institute. Specific operating procedures were performed according to the kit instructions. The results are shown in Table 1.

[0049] Table 1. Results Statistics Table

[0050]

[0051] IgG is the most concentrated immunoglobulin in fluids, possessing antibacterial and antiviral effects. It can also neutralize toxins, rendering them inactive, and opsonize, agglutinate, and precipitate antigens, playing a crucial role in humoral immunity. IgM binds to complement after infection, exhibiting a strong ability to dissolve pathogens and acting as a "pioneer" in anti-infection efforts. It also possesses multiple functions including precipitation, agglutination, complement fixation, and viral neutralization. IgA is the main immunoglobulin in exocrine fluids, excreted with secretions to the mucosal surface, exerting antibacterial and antiviral effects. The concentration or titer of serum immunoglobulins reflects the level of humoral immunity in the body; a higher concentration or titer indicates a higher level of humoral immunity.

[0052] As can be seen from Table 1, the concentration of serum immunoglobulin in piglets fed with the antiviral powder in Examples 1-3 was significantly higher than that in the comparative example, indicating that the use of the antiviral powder provided by the present invention can effectively improve the humoral immune function of piglets. From the comparison between the examples and the comparative example, the composition of the antiviral powder has a certain influence on improving the concentration of serum immunoglobulin in piglets.

[0053] Subsequently, to verify the effect of the selection of fermentation strains on the antiviral effect of traditional Chinese medicine fermentation powder, the selection of fermentation strains was adjusted according to Table 1. Bacillus berleis and Candida utilis were both available through commercial channels.

[0054] Table 1. Selection of Fermentation Strains

[0055]

[0056] (1) Antibacterial test of pathogens causing diarrhea in piglets

[0057] All the piglets tested exhibited vomiting and diarrhea to some extent, with loose, foul-smelling feces, short-term fever, weight loss, thirst, and obvious dehydration. They were diagnosed with viral gastroenteritis in piglets, and the cause may be poor feeding environment, improper feeding management, and mixed infection of multiple pathogens.

[0058] Feces from diarrheal piglets were collected using sterile cotton swabs and spread onto MacConkey agar plates. Colony morphology was observed, examined under a microscope, and physiological and biochemical identification was performed. The microscopic morphology of the colonies in the MacConkey agar plates matched the characteristics of *E. coli*. The identified colonies were cultured to 10... 9 -10 10 The antibacterial test was conducted using the above-mentioned fermented Chinese medicine powder at CFU / mL, with ceftriaxone as control 1. The fermented Chinese medicine powder was soaked in hot water extract, and the antibacterial test was performed according to the conventional KB method, measuring the size of each inhibition zone. Judgment criteria: greater than 15 mm was highly sensitive; 10-15 mm was moderately sensitive; less than 10 mm was low sensitive. Ten replicates were performed for each group, and the results were averaged.

[0059] The results are shown in Table 2.

[0060] Table 2. Results Statistics Table

[0061]

[0062] As shown in Table 2, Escherichia coli showed moderate to high sensitivity to the fermented Chinese medicine powder, with an inhibition zone size between 13.7 and 15.8 mm. Although this is not as effective as common cephalosporin antibiotics, it still has clinical antibacterial value. In addition, the data comparison between groups showed that the antibacterial effect of the fermented Chinese medicine powder prepared by the combined fermentation of Bacillus berreatus and Candida utilis was better than that of Bacillus berreatus and Candida utilis alone. The antibacterial effect was best when the ratio of Bacillus berreatus to Candida utilis in the bacterial solution was 2:1.

[0063] (2) Therapeutic efficacy

[0064] Fermentation strains were selected according to Table 1, and antiviral powders for each group were prepared according to the preparation method given in Example 1. The therapeutic efficacy of each group of antiviral powders was verified. A total of 500 diseased piglets were tested, with 50 piglets in each group.

[0065] For piglets still with appetite, antiviral powder was mixed into feed at a dosage of 5 g / kg. Control 2 used dysentery-fighting agent mixed into feed at a dosage of 5 g / kg. Control 3 used a mixture of unfermented Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn, and Polygonatum sibiricum powder instead of the fermented Chinese medicine powder, prepared according to the method in Example 1, and mixed into feed at a dosage of 5 g / kg. For piglets with no appetite, the antiviral powder was administered orally at the same dosage. Control 2 received 2-4 mL of ribavirin intravenously, and Control 3 received the same dosage orally. All treatments were administered continuously for 3-5 days, 1-2 times daily. The number of piglets treated, the efficacy, and the proportion of effective treatments were recorded for each group. The condition was followed up for one week after recovery to observe for relapse.

[0066] Criteria for judging efficacy: (1) Death: The course of treatment has not been completed and the sick pigs have died; (2) Ineffective: The clinical symptoms have not been well controlled and the incidence of the disease in the group has increased; (3) Effective: The clinical symptoms have been effectively controlled and the symptoms have been reduced or improved; (4) Cured: The disease symptoms have disappeared, the group symptoms have been effectively controlled, the feces are formed and the color is normal, and drinking water and eating have basically recovered.

[0067] The results are shown in Table 3.

[0068] Table 3. Results Statistics Table

[0069]

[0070]

[0071] As can be seen from Table 3, the antiviral powder made with fermented Chinese medicine powder as the component of the antiviral powder was more effective in treating viral gastroenteritis in piglets than conventional drug treatment (Control 2) and antiviral powder made by replacing the fermented Chinese medicine powder with a mixture of unfermented Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum (Control 3).

[0072] Furthermore, data comparison between groups shows that the antiviral powder prepared by fermenting traditional Chinese medicine using Bacillus berleis and Candida utilis in combination has a better therapeutic effect than that prepared using Bacillus berleis and Candida utilis alone.

[0073] In addition, no recurrence of the disease was found in the cured piglets one week later, indicating that the treatment had a good effect and the clinical symptoms were effectively controlled. Piglets with reduced or improved symptoms continued to be fed antiviral powder for 7-10 days. The symptoms disappeared, the feces became formed and normal in color, and the piglets' drinking and eating basically recovered.

[0074] The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A veterinary immune-enhancing and antiviral powder, characterized in that: By weight percentage, it consists of 60-70% Chinese herbal fermentation powder, 20-30% tung seed oil, and 10-20% yam polysaccharide; The Chinese herbal fermentation powder is made by first fermenting Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum using Bacillus belye and Candida utilis to obtain fermented products, and then drying and ultra-fine pulverizing the fermented products. The mass ratio of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn, and Polygonatum sibiricum is (20-30):(20-30):(5-10):(1-5):(1-5); The ratio of Bacillus belye and Candida utilis in the bacterial culture is 2:1, and the total inoculation amount of Bacillus belye and Candida utilis is 3-5% of the total mass of the fermentation material.

2. A method for preparing a veterinary immune-enhancing antiviral powder as described in claim 1, characterized in that: Includes the following steps: (1) Bacillus belye and Candida utilis were activated and proliferated to 10 by liquid culture. 9 -10 10 CFU / mL, and then the bacterial solution was inoculated into a mixture of Scutellaria baicalensis, Astragalus membranaceus, pomegranate peel, hawthorn and Polygonatum sibiricum, and the water content was controlled at 30-40% for solid-state anaerobic fermentation to obtain the fermentation product; (2) The fermented material obtained in step (1) is dried and then subjected to one airflow ultrafine pulverization to obtain Chinese herbal fermentation powder; (3) Add the evenly mixed tung seed oil and yam polysaccharide to the fermented Chinese medicine powder obtained in step (2), and then pulverize it through secondary airflow to obtain a veterinary immune-enhancing antiviral powder.

3. The method for preparing a veterinary immune-enhancing antiviral powder according to claim 2, characterized in that: In step (1), the fermentation temperature is 20-30℃ and the fermentation time is 15-20 days.

4. The method for preparing a veterinary immune-enhancing antiviral powder according to claim 2, characterized in that: The process conditions for the primary airflow ultrafine pulverization are: airflow temperature of 15-20℃, airflow pressure of 7-10 bar, pulverization speed of 8-10 r / min, and pulverization time of 20-25 min; the process conditions for the secondary airflow ultrafine pulverization are: airflow temperature of 10-15℃, airflow pressure of 2-5 bar, pulverization speed of 2-6 r / min, and pulverization time of 10-15 min.

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