Method for rapid dry pig manure biomass based on bacillus thermophilus metabolism
By using thermophilic Bacillus synergistic metabolism, the problems of low energy consumption and pollution-free rapid drying of pig manure have been solved, producing high-calorific-value biomass fuel raw materials.
Patent Information
- Application Number
- CN202311489298.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-10
- Publication Date
- 2025-12-12
- Estimated Expiration
- 2043-11-10
AI Technical Summary
Existing technologies are insufficient for quickly and efficiently drying pig manure, leading to environmental pollution and high energy costs.
The thermophilic Bacillus strains (Bacillus martensii, Bacillus amyloliquefaciens, and Bacillus methyltrophicus) are used for synergistic metabolism. By stirring, the moisture content of pig manure is reduced, and the exothermic metabolic characteristics of the thermophilic strains are utilized to achieve rapid drying.
This process achieves low-energy, odorless drying of pig manure, producing high-calorific-value biomass fuel feedstock, and reducing ammonia emissions and environmental pollution.
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Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the field of pig manure-based biomass fuel raw materials, and particularly relates to a method for producing pig manure-based biomass fuel raw materials based on Bacillus metabolism and rapid drying. BACKGROUND
[0002] In recent years, the livestock and poultry breeding industry in various regions has gradually developed in a large-scale direction, and the disposal method and application convenience of manure waste have been important factors affecting its development. On the one hand, the treatment of livestock and poultry breeding manure is still oriented to "return to field utilization" and "integration of breeding and planting", and it is usually difficult to match sufficient land for disposal. In addition, the process of returning to field requires high labor cost, which makes it difficult to completely open up the channel for manure return to field. On the other hand, due to various limitations, it is also difficult to realize the centralized treatment of manure from regional breeding enterprises. Therefore, finding new treatment technologies has become a problem to be solved, and high-value resourceization is an effective way out.
[0003] Pig manure is mainly composed of feed raw materials such as bran that have not been metabolized, and it is essentially a mixed biomass. Studies have shown that the combustion of one kilogram of dry pig manure can produce 3500-4500 large calories of heat, which is about half of the standard coal. In addition, in many regions of China, pig houses need artificial heating for a period of time every year, which requires fuel. Therefore, its development as a biomass fuel raw material has important prospects, which can not only realize high-value utilization on site, but also reduce the dependence on coal resources. Since pig manure contains a high water content, in order to make it a good pig manure-based biomass raw material, it is necessary to solve the problem of rapid drying of pig manure to avoid the generation of odor and product sludge due to fermentation.
[0004] At present, the manure produced by pig breeding generally adopts a dry-wet separation process, that is, a method of collecting fresh manure and part of urine separated from flushing water, but the water content of the obtained fresh pig manure is still 40% to 60%. If it is simply dried or stacked, the fresh pig manure often contains a certain amount of feed that has not been absorbed and a large number of microorganisms brought from the intestinal tract, which is easy to further decompose and produce odor. At the same time, the fresh pig manure may also contain part of viruses and harmful pathogenic bacteria, so such treatment is easy to pollute the environment, and therefore rapid drying is urgently needed. At present, the drying technology, such as the pig manure drying room disclosed in CN217651100U, mainly adopts a heating drying method, which has high energy consumption cost. According to the water evaporation energy consumption calculation, 120 degrees of electricity or more is needed to consume 1 ton of water. In addition, uneven heating during drying is easy to discharge odor, which affects the surrounding environment, so it is urgent to develop a drying technology with relatively low energy consumption and small environmental impact.
[0005] In recent years, people have found that there are thermophilic microorganisms in the aerobic fermentation process of compost fermentation research. The fermentation process can generate heat, and a new technology of super high temperature composting has been developed, which has been successfully applied to the treatment and resource utilization of organic waste such as municipal sludge. The process is based on the super high temperature aerobic fermentation inoculum rich in extreme thermophilic microorganisms, which can quickly raise the compost temperature to above 80℃ (the highest temperature can reach 100℃) without relying on external heat sources, and can last for 5-7 days. However, in order to promote the complete decomposition of the material, the moisture content of the material needs to be maintained at 60-70% for a long time. Once the humidity is lower than the above range, the heat production effect of thermophilic bacteria will rapidly decrease. Because the existing super high temperature composting thermophilic microorganisms show high activity and need to maintain the moisture content of the material at 60-70%, and the moisture content of pig manure gradually decreases during the drying process, most thermophilic microorganisms are difficult to maintain persistent activity, so there is currently a lack of research on using such strains for rapid drying of pig manure.
[0006] Bacillus is a very important species of microbial deodorization bacteria. It can produce various extracellular enzymes such as protease, lipase, amylase, pectinase and cellulase, etc., which can decompose complex plant carbohydrates. At the same time, Bacillus can also inhibit the growth and reproduction of Escherichia coli and other gram-negative bacteria, reduce the urease activity produced by gram-negative bacteria, thereby reducing the decomposition of nitrogen-containing substances in feces by urease, leading to nitrogen loss and reducing the ammonia nitrogen concentration in feces. It can also inhibit the activity of Escherichia coli and convert proteins into amines and ammonia, and has strong deodorization function. Studies have found that Bacillus is a representative bacterium in the high-temperature stage of composting, and many populations have thermophilic characteristics. They have very thick spore walls to resist high temperatures, radiation and chemical corrosion in adverse environments, and have good application prospects in composting. For example, Bacillus marcorestinctum can maintain a compost temperature above 80℃ and significantly reduce ammonia emissions (Yan Wang et al., Ecotoxicology and Environmental Safety, 2019, 180:80-87). Wang Peng et al. found that the optimal activity temperature of cellulase produced by the screened thermophilic Bacillus was 80℃, and it still maintained 60% activity at 100℃ (Jiangsu Agricultural Science, 2012, 40(9):348-350).
[0007] Our research team has been focusing on beneficial microorganisms for a long time, so we have obtained the following three strains and applied for the corresponding patents, as follows:
[0008] The invention "Bacillus marcorestinctum L-YQ isolated from smelting plant soil and its application" of CN112877256B provides: Bacillus marcorestinctum L-YQ, with preservation number: CGMCC No. 20849. It is used for treating wastewater containing n-butanol.
[0009] The invention of CN107267412B provides Bacillus methylotrophicus LM-N with preservation number CGMCC No.13541. It is used for preparing a plant pathogen fungicide.
[0010] The invention of CN107090419B provides Bacillus amyloliquefaciens LM-Y with preservation number CGMCC No.13542. It is used for preparing a plant pathogen fungicide.
[0011] The invention of CN112931703B provides a selenium-rich polypeptide protein feed based on intestinal beneficial Bacillus, which is composed of the following ingredients: 2.0-3.3 kg of sodium selenite, 30 kg of calcium carbonate, 100-150 kg of corn flour or wheat flour, 500-600 kg of soybean meal, 100-150 kg of rapeseed meal, 100-150 kg of Broussonetia papyrifera leaves, 150 kg of water, 100-150 kg of Bacillus marcorestinctum sp. L-YQ bacterial liquid, 50-150 kg of Bacillus amyloliquefaciens LM-Y bacterial liquid, and 50-150 kg of Bacillus methylotrophicus LM-N bacterial liquid. The colony content of the L-YQ bacterial liquid is about 5-12 billion / mL. The colony content of the LM-Y bacterial liquid is about 3-8 billion / mL. The colony content of the LM-N bacterial liquid is about 3-8 billion / mL. The invention is based on the synergistic transformation of intestinal beneficial Bacillus, effectively improving the conversion efficiency of inorganic selenium, and realizing selenium supplementation while simultaneously promoting intestinal flora health regulation to reduce antibiotic use. SUMMARY
[0012] The technical problem to be solved by the present application is to provide a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism.
[0013] To solve the above technical problems, the present application provides a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, comprising the following steps:
[0014] 1) Detect the moisture content of fresh pig manure. When the moisture content of fresh pig manure is ≤40%, directly use 1000 kg of fresh pig manure as raw material;
[0015] Conversely, when the moisture content of the fresh pig manure is > 40%, dry sawdust (moisture content ≤ 10%) is added as raw material in 1000 kg of fresh pig manure, and the moisture content of the raw material is controlled at 39-41% (preferably 40%);
[0016] 2) 5-30 kg of Bacillus marcorestinctum liquid, 5-35 kg of Bacillus amyloliquefaciens liquid, and 5-50 kg of Bacillus methylotrophicus liquid are added (in batches) to the raw material obtained in step 1), and the mixture is thoroughly mixed to form a mixed material;
[0017] The mixed material is stirred at a stirring speed of 40-60 revolutions per minute until the moisture content is ≤ 15%, and a pig manure-based biomass raw material is obtained.
[0018] As an improvement of the method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism according to the present application, the pig manure-based biomass raw material can be used to produce biomass fuel rods.
[0019] As a further improvement of the method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism according to the present application, the fresh pig manure is fresh pig manure obtained by dry-wet separation (its moisture content is generally about 40-60%).
[0020] As a further improvement of the method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism according to the present application, in step 2), after reaching the maximum temperature (the maximum temperature is about 70-80°C, and the required time is about 10-12 hours) during the stirring process (stirring speed of 40-60 revolutions per minute), the stirring state is still maintained at 40-60 revolutions per minute until the moisture content is ≤ 15% (about 36 hours are required), and a pig manure-based biomass raw material is obtained.
[0021] As a further improvement of the method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism according to the present application, step 2) is carried out in an open stirring barrel (3000 L) placed in a sunlight room.
[0022] As a further improvement of the method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism according to the present application,
[0023] The Bacillus marcorestinctum is Bacillus marcorestinctum L-YQ with the preservation number CGMCC No. 20849;
[0024] The Bacillus methylotrophicus is Bacillus methylotrophicus LM-N with the preservation number CGMCC No. 13541;
[0025] The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens with a preservation number of CGMCC No. 13542.
[0026] As a further improvement of the method for producing pig manure biomass fuel based on Bacillus amyloliquefaciens metabolism:
[0027] The viable cell content of the Bacillus marisflavi L-YQ bacterial solution is 1-1.2 billion / mL, the viable cell content of the Bacillus amyloliquefaciens LM-Y bacterial solution is 0.6-0.8 billion / mL, and the viable cell content of the Bacillus methylotrophicus LM-N bacterial solution is 0.6-0.8 billion / mL.
[0028] As a further improvement of the method for producing pig manure biomass fuel based on Bacillus amyloliquefaciens metabolism:
[0029] The sum of the weights of the Bacillus marisflavi L-YQ bacterial solution, the Bacillus amyloliquefaciens LM-Y bacterial solution, and the Bacillus methylotrophicus LM-N bacterial solution is 50±2 kg.
[0030] As a further improvement of the method for producing pig manure biomass fuel based on Bacillus amyloliquefaciens metabolism:
[0031] 5-10 kg of the Bacillus marisflavi bacterial solution, 20-35 kg of the Bacillus amyloliquefaciens bacterial solution, and 5-20 kg of the Bacillus methylotrophicus bacterial solution are added to the raw material obtained in step 1).
[0032] The preparation method of the above bacterial solution refers to the invention “Selenium-rich polypeptide protein feed based on intestinal beneficial Bacillus and production method thereof” of CN112931703B.
[0033] The present application finds that the combination of Bacillus marisflavi L-YQ, Bacillus amyloliquefaciens LM-Y bacterial solution, and Bacillus methylotrophicus LM-N can maintain metabolic heat release when the water content gradually decreases.
[0034] It should be emphasized that CN112931703B is established based on the material transformation characteristics of the metabolism of the above three beneficial Bacillus, while the present application takes full advantage of the fact that the combination of the above three beneficial Bacillus can maintain metabolic heat release when the water content gradually decreases, and constructs a method for producing pig manure biomass fuel based on Bacillus amyloliquefaciens metabolism.
[0035] The method of the present application is a production method based on the synergistic metabolism of beneficial thermophilic bacillus (Bacillus marinus L-YQ, Bacillus amyloliquefaciens LM-Y bacterial solution, methylotrophic bacillus LM-N) to promote the rapid vaporization of residual moisture in fresh pig manure after dry-wet separation into the atmosphere, accelerate the drying of pig manure, and obtain a production method for producing pig manure-based biomass fuel raw materials. Compared with the traditional drying method, the present application has no odor, low energy consumption cost, and the obtained biomass fuel raw material has high and stable calorific value. The drying process of the present application can deodorize and reduce ammonia emission, so it basically has no odor. DETAILED DESCRIPTION
[0036] The present application will be further described below in conjunction with specific examples, but the protection scope of the present application is not limited to this:
[0037] I. The bacterial solution used in the present application:
[0038] The colony content of Bacillus marinus L-YQ bacterial solution is about 10-12 billion / mL, the colony content of Bacillus amyloliquefaciens LM-Y bacterial solution is about 6-8 billion / mL, and the colony content of methylotrophic bacillus LM-N bacterial solution is about 6-8 billion / mL.
[0039] The preparation method of the above bacterial solution refers to the invention "Selenium-rich polypeptide protein feed based on intestinal beneficial bacillus and its production method" of CN112931703B.
[0040] II. The determination of the moisture content of the fresh pig manure obtained by dry-wet separation is a conventional method, for example, the vacuum oven drying method (Bao Shidang, Soil and Agricultural Chemistry Analysis (Third Edition), China Agriculture Press, 2000) can be used.
[0041] The present application is carried out at a conventional room temperature.
[0042] Example 1, a method for rapidly drying pig manure biomass based on thermophilic bacillus metabolism, the following steps are carried out in turn:
[0043] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000kg of fresh pig manure obtained by dry-wet separation with a water content of 40% determined by the drying method was added in batches, and stirred and loosened; this was used as the raw material;
[0044] 2) to the raw material obtained in step 1), 5 kg of Bacillus marinus L-YQ bacterial solution, 25 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, and 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 50 revolutions per minute, and the material is uniformly stirred for about 10.0 hours until the material temperature reaches the highest (78℃), and then the stirring speed is maintained at 50 revolutions per minute until the water content of the material is reduced to ≤15%, and thus after about 36 hours, the stirring is stopped, and pig manure-based biomass raw material for producing biomass fuel rods is obtained, and then a fuel rod forming machine is used to extrude and solidify to form pig manure-based biomass fuel rods.
[0045] During the preparation of the present application, there is no obvious odor.
[0046] Example 2, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0047] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 50% determined by the drying method is added in batches, and stirred and loosened, and then about 167 kg of dry sawdust is added in batches while stirring, and mixed uniformly to obtain a raw material with a water content of 40%;
[0048] 2) to the raw material obtained in step 1), 5 kg of Bacillus marinus L-YQ bacterial solution, 25 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, and 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 50 revolutions per minute, and the material is uniformly stirred for about 11 hours until the material temperature reaches the highest (76℃), and then the stirring speed is maintained at 50 revolutions per minute, and after about 36 hours, the water content of the material is reduced to ≤15%, and the stirring is stopped, and pig manure-based biomass raw material for producing biomass fuel rods is obtained, and then a fuel rod forming machine is used to extrude and solidify to form pig manure-based biomass fuel rods.
[0049] During the preparation of the present application, there is no obvious odor.
[0050] Example 3, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0051] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 60% determined by the drying method is added in batches, and stirred and loosened, and then about 318 kg of dry sawdust is added in batches while stirring, and mixed uniformly to obtain a raw material with a water content of 40%;
[0052] 2) to the raw material of step 1), 5 kg of Bacillus marcusii L-YQ bacterial solution, 25 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 50 revolutions per minute, and after about 12 hours, the material temperature reaches the highest (77 DEG C), then the stirring speed is maintained at 50 revolutions per minute, and after about 36 hours, the moisture content of the material is reduced to ≤15%, and the stirring is stopped, thereby obtaining pig manure-based biomass raw material that can be used to produce biomass fuel rods, and then a fuel rod forming machine is used to extrude and solidify to form pig manure-based biomass fuel rods.
[0053] During the preparation of the application, there is no obvious odor.
[0054] Example 4, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0055] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 40% determined by the drying method is added in batches, and is stirred and loosened; and the same is used as raw material;
[0056] 2) to the raw material obtained in step 1), 5 kg of Bacillus marcusii L-YQ bacterial solution, 25 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 40 revolutions per minute, and after about 12 hours, the material temperature reaches the highest (76 DEG C), then the stirring speed is maintained at 40 revolutions per minute, and after about 36 hours, the moisture content of the material is reduced to ≤15%, and the stirring is stopped, thereby obtaining pig manure-based biomass raw material that can be used to produce biomass fuel rods, and then a fuel rod forming machine is used to extrude and solidify to form pig manure-based biomass fuel rods.
[0057] During the preparation of the application, there is no obvious odor.
[0058] Example 5, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0059] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 40% determined by the drying method is added in batches, and is stirred and loosened; and the same is used as raw material;
[0060] 2) to the raw material obtained in step 1), 10 kg of Bacillus marinus L-YQ bacterial solution, 20 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, and 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 50 revolutions per minute, and after about 10.4 hours, the material temperature reaches a maximum of 77℃, and then the stirring speed is maintained at 50 revolutions per minute, and after about 36 hours, the moisture content of the material is reduced to ≤15%, and the stirring is stopped, and pig manure-based biomass raw material for producing biomass fuel rods is obtained, and then a fuel rod forming machine is used to extrude and solidify and form pig manure-based biomass fuel rods.
[0061] During the preparation process of the present application, there is no obvious odor.
[0062] Example 6, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0063] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 40% determined by the drying method is added in batches, and is stirred and loosened; and this is used as the raw material;
[0064] 2) to the raw material obtained in step 1), 10 kg of Bacillus marinus L-YQ bacterial solution, 20 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, and 20 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 60 revolutions per minute, and after about 10.2 hours, the material temperature reaches a maximum of 77℃, and then the stirring speed is maintained at 60 revolutions per minute, and after about 36 hours, the moisture content of the material is reduced to ≤15%, and the stirring is stopped, and pig manure-based biomass raw material for producing biomass fuel rods is obtained, and then a fuel rod forming machine is used to extrude and solidify and form pig manure-based biomass fuel rods.
[0065] During the preparation process of the present application, there is no obvious odor.
[0066] Example 7, a method for rapidly drying pig manure biomass based on Bacillus thermophilus metabolism, the following steps are sequentially performed:
[0067] 1) In a 3000L open stirring barrel placed in a sunlight room, 1000 kg of fresh pig manure obtained by dry-wet separation with a water content of 40% determined by the drying method is added in batches, and is stirred and loosened; and this is used as the raw material;
[0068] 2) to the raw material obtained in step 1), 10 kg of Bacillus marinus L-YQ bacterial solution, 35 kg of Bacillus amyloliquefaciens LM-Y bacterial solution, and 5 kg of Bacillus methylotrophicus LM-N bacterial solution are added, and after being fully mixed, the stirring speed is maintained at 50 revolutions per minute, and after about 11.2 hours, the material temperature reaches the highest (76℃), and then the stirring speed is maintained at 50 revolutions per minute, and after about 36 hours, the moisture content of the material is reduced to ≤15%, and the stirring is stopped, and the pig manure-based biomass raw material for producing biomass fuel rods is obtained, and then a fuel rod forming machine is used to extrude and solidify to produce pig manure-based biomass fuel rods.
[0069] In the preparation process of the present application, there is no obvious odor.
[0070] In experiment one, the calorific value characteristics of the pig manure-based biomass fuel rods obtained in examples 1-7 were determined by the solid biomass fuel calorific value determination method (GB / T 30727-2014), and the determination results are shown in Table 1.
[0071] Table 1 Calorific value characteristics and energy consumption characteristics of fuel rods prepared from pig manure-based biomass fuel raw materials produced by Bacillus thermophilus metabolic temperature rise rapid drying
[0072]
[0073] As can be seen from the results in Table 1, the pig manure-based biomass fuel raw materials produced by Bacillus thermophilus metabolic temperature rise rapid drying in examples 1-7 have a calorific value of 3810-4200 kcal / kg, and the calorific value characteristics of the fuel rods prepared therefrom are relatively stable, and there is no significant difference in calorific value after 720 days of conventional storage compared with the original data. Therefore, the prepared pig manure-based biomass fuel can be completely utilized as biomass fuel. Compared with the traditional drying method, the traditional drying method needs about 120 degrees of electricity to remove 1 ton of water, while the energy consumption for drying 1 ton of water in the present application is mainly caused by the stirrer, which is an average of 46 degrees of electricity, which is 38.33% of the traditional method. Therefore, the present method is a low-energy fresh pig manure drying method.
[0074] Comparative example 1: relative to example 1, the amounts of Bacillus marinus L-YQ bacterial solution, Bacillus amyloliquefaciens LM-Y bacterial solution, and Bacillus methylotrophicus LM-N bacterial solution are changed; the stirring speed is unchanged; the whole stirring reaction time is as shown in Table 2; and the results obtained are as shown in Table 2.
[0075] Table 2
[0076]
[0077] From the results of the above table, when only two kinds of bacteria work together (Comparative Examples 1, 3, 4), the highest temperature is significantly lower than the present application, and the total drying time is much longer than the present application, and the energy consumption is not much different from direct drying, and there is a little odor, so two-by-two combinations cannot achieve the technical goal of fast, low energy consumption, and no secondary pollution. In addition, from Comparative Example 2, when three kinds of bacteria work together, there is no obvious odor, so the synergistic work of the three is helpful to the control of odor, and also indicates that there is a co-metabolic relationship between the three, but when the amount of the three is too large, the co-metabolic balance of each other is broken, resulting in a decrease in heat production efficiency.
[0078] Comparative Example 5, relative to Example 2, cancels the use of "167 kg of dried sawdust" in step 1), i.e., using fresh pig manure with a water content of 50% as the raw material to directly perform step 2); the rest is the same as Example 2.
[0079] The results obtained are: uniform stirring for about 15.0 hours, the material temperature reaches a maximum of 70℃, then maintain the stirring state for about 48 hours, the water content of the material is reduced to ≤15%, the energy consumption is about 62 degrees of electricity per ton of water, and the biomass heat value formed is 3730 kcal / kg. Its performance is obviously not as good as Example 2 of the present application.
[0080] Finally, it should also be noted that the above only lists several specific embodiments of the present application. Obviously, the present application is not limited to the above embodiments, but can also have many variations. All variations that can be directly derived or inferred from the content disclosed by a person of ordinary skill in the art should be considered as falling within the scope of protection of the present application.
Claims
1. A method for rapidly drying pig manure biomass based on the metabolism of thermophilic Bacillus, characterized in that... Includes the following steps: 1) Test the moisture content of fresh pig manure. When the moisture content of fresh pig manure is ≤40%, use 1000 kg of fresh pig manure directly as raw material. Conversely, when the moisture content of fresh pig manure is >40%, dry sawdust is added to 1000 kg of fresh pig manure as raw material, and the moisture content of the raw material is controlled to be 39-41%. 2) Add 5-30 kg of Bacillus martensii solution, 5-35 kg of Bacillus amyloliquefaciens solution, and 5-50 kg of Bacillus methyltrophicus solution to the raw materials obtained in step 1), and mix thoroughly to form a mixture. The mixture is placed in an open mixing tank in a sunroom and stirred at a speed of 40-60 revolutions per minute until the moisture content is ≤15%, thus obtaining pig manure-based biomass raw material. Bacillus marcorestinctum is the Bacillus marcorestinctum L-YQ with accession number CGMCC No. 20849; The methylotrophic Bacillus is Bacillus methylotrophicus LM-N, with preservation number CGMCC No. 13541; The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens LM-Y, with preservation number CGMCC No. 13542.
2. The method for rapidly drying pig manure biomass based on the metabolism of thermophilic Bacillus as described in claim 1, characterized in that: The pig manure-based biomass feedstock can be used to produce biomass fuel rods.
3. The method for rapidly drying pig manure biomass based on the metabolism of Bacillus thermophilus according to claim 1 or 2, characterized in that: The fresh pig manure refers to fresh pig manure obtained through dry-wet separation.
4. The method for rapidly drying pig manure biomass based on the metabolism of thermophilic Bacillus as described in claim 3, characterized in that: Step 2): During the mixing process, after the mixture reaches the highest temperature, it is still maintained at a stirring speed of 40-60 revolutions per minute until the moisture content is ≤15%, thus obtaining pig manure-based biomass raw material.
5. The method for rapidly drying pig manure biomass based on the metabolism of thermophilic Bacillus as described in claim 4, characterized in that: The viable cell count of Bacillus martensii L-YQ culture is 10-12 billion / mL, the viable cell count of Bacillus amyloliquefaciens LM-Y culture is 6-8 billion / mL, and the viable cell count of Bacillus methyltrophicus LM-N culture is 6-8 billion / mL.
6. The method for rapidly drying pig manure biomass based on the metabolism of Bacillus thermophilus according to claim 5, characterized in that: The total weight of the Bacillus martensii L-YQ culture, Bacillus amyloliquefaciens LM-Y culture, and Bacillus methyltrophicus LM-N culture was 50 ± 2 kg.
7. The method for rapidly drying pig manure biomass based on the metabolism of Bacillus thermophilus according to claim 6, characterized in that: Add 5-10 kg of Bacillus martensii solution, 20-35 kg of Bacillus amyloliquefaciens solution, and 5-20 kg of Bacillus methyltrophicus solution to the raw materials obtained in step 1).
Citation Information
Patent Citations
Bacillus amyloliquefaciens and its applications
CN107090419B
Methyltrophic Bacillus and its applications
CN107267412B
Bacillus martensii L-YQ isolated from smelter soil and its application
CN112877256B
Selenium-enriched polypeptide protein feed based on beneficial gut Bacillus and its production method
CN112931703B
Pig manure drying room
CN217651100U