An anti-inflammatory repair composition and uses thereof

Abstract

The application relates to the cosmetic technical field, and particularly discloses an anti-inflammatory repair composition and application thereof. The anti-inflammatory repair composition is composed of centella asiatica extract and peony root bark extract, and the mass ratio of the centella asiatica extract to the peony root bark extract is 1:(0.5-1). The anti-inflammatory repair composition provided by the application is compounded by the centella asiatica extract and the peony root bark extract, realizes anti-inflammatory repair of skin through different mechanisms, and the two can synergistically enhance the anti-inflammatory effect, so that the anti-inflammatory repair composition has a good application prospect in cosmetics.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to an anti-inflammatory and repairing composition, its preparation method, and its application. Background Technology

[0002] The skin barrier comprises physical, chemical, microbial, and immune barriers, playing a crucial role in protecting the body from harmful factors such as chemical (e.g., detergents, soaps), physical (e.g., temperature, radiation, mechanical damage), and microbial stimuli, as well as maintaining relative internal homeostasis. Damage to the skin barrier leads to skin sensitivity and fragility, causing significant moisture loss and triggering inflammation upon external stimuli. Skin inflammation occurs when skin tissue is stimulated by external factors, causing keratinocytes, the main cells of the epidermis, to release pro-inflammatory factors (e.g., IL-1α, TNFα) and chemokines (e.g., IL-8). These pro-inflammatory factors further bind to corresponding receptors on the cell membrane, activating inflammation-related signaling pathways (e.g., NF-κB), resulting in the production of more inflammatory factors. This amplifies the inflammatory cascade, inducing vascular reactions and symptoms such as erythema. In recent years, inflammatory skin diseases caused by environmental pollution, life stress, and cosmetic intolerance have been on the rise, causing significant disruption to people's lives. Therefore, the development of cosmetic ingredients and cosmetics with anti-inflammatory and repairing effects for people with inflammatory skin is receiving increasing attention and favor from the industry and consumers.

[0003] Centella asiatica is the dried whole herb of the plant Centella asiatica, belonging to the Apiaceae family. Clinically, it is widely used for anti-inflammatory, anti-ulcer, and wound-healing purposes. Centella asiatica contains active triterpenoid saponins, primarily asiaticoside, asiaticoside, and asiatic acid. Current techniques for preparing Centella asiatica extracts mostly employ solvent extraction and resin purification methods. Solvent extraction generally involves high temperatures, which accelerates the hydrolysis of asiaticoside and asiaticoside, affecting the yield. It also consumes a significant amount of energy, hindering energy conservation. Subsequent purification processes, such as resin enrichment and crystallization, are numerous, lack automation, and are difficult to scale up for industrial production. Domestically produced anti-inflammatory compositions containing asiaticoside primarily repair the skin's physical barrier, without addressing the simultaneous repair of the microbial barrier and the acidic chemical barrier.

[0004] Peony root bark extract is a natural plant extract derived from peony root bark. Its main components include various active substances with multiple biological activities such as antioxidation, antibacterial, and anti-inflammatory properties. The main effective substances in peony root bark extract include paeoniflorin, saponins, polyphenols, and flavonoids. These components possess various biological activities such as antioxidation, antibacterial, and anti-inflammatory properties, and can be used to prevent and improve oxidative stress-related diseases and inflammatory diseases. They can also be used in skincare product formulations to improve dry and aging skin. Paeonol is the main anti-inflammatory component in peony root bark extract. It is readily soluble in ethanol and slightly soluble in water. Paeonol has analgesic and anti-inflammatory effects. In daily chemical applications, paeonol can also inhibit intracellular O2. 2- The generation of free radicals can whiten the skin, reduce and fade pigment deposits in the skin, and has effects such as fading bruises and spots, reducing inflammation, swelling and pain, anti-allergy, and antiviral.

[0005] There are many causes of skin inflammation. Although asiaticoside and paeonol both have certain anti-inflammatory effects, their individual effects are relatively weak. Furthermore, when a single component acts on the skin, it cannot achieve different mechanisms of action to anti-inflammate and repair the skin. Therefore, a new method is needed to address these issues. Summary of the Invention

[0006] To overcome the above-mentioned technical defects, this application provides an anti-inflammatory repair composition and its application. The composition uses Centella asiatica extract and Paeonia suffruticosa root bark extract to achieve anti-inflammatory and skin repair through different mechanisms of action. Moreover, the combination of the two can synergistically enhance the anti-inflammatory effect and has good application prospects in cosmetics.

[0007] To achieve the above-mentioned technical objectives, this application provides an anti-inflammatory repair composition and its application, employing the following technical solution:

[0008] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract, wherein the mass ratio of Centella asiatica extract to Paeonia suffruticosa root bark extract is 1:(0.5-1).

[0009] Preferably, the mass ratio of the Centella asiatica extract to the peony root bark extract is 1:0.8.

[0010] By adopting the above technical solution, the main anti-inflammatory components of Centella asiatica extract and Paeonia suffruticosa root bark extract are asiaticoside and paeonol, respectively, and the combination of the two has a good synergistic anti-inflammatory effect. In addition, Centella asiatica extract and Paeonia suffruticosa root bark extract also contain other ketones, phenols, and glycosides with anti-inflammatory effects. The above composition provided in this application can promote the secretion of anti-inflammatory factors that repair the skin barrier by skin cells and inhibit the expression of inflammatory factors IL-1α and IL-6 to exert anti-inflammatory effects.

[0011] More preferably, the preparation method of the Centella asiatica extract is as follows:

[0012] A1. Degrease the Centella asiatica powder to obtain defatted Centella asiatica powder;

[0013] A2. Mix defatted Centella asiatica powder with water, add cellulase, hemicellulase and pectinase, and extract by ultrasonication to obtain a mixture;

[0014] A3. Alkaline protease was added to the mixture for enzymatic hydrolysis, the supernatant was collected by ultrafiltration, and the mixture was concentrated under vacuum and freeze-dried to obtain Centella asiatica extract.

[0015] Preferably, the weight ratio of cellulase, hemicellulase, and pectinase is 5:3:2.

[0016] By adopting the above technical solution, this application first treats defatted Centella asiatica powder with cellulase to make its effective substances more easily permeate and be extracted, and then uses alkaline protease to enzymatically hydrolyze it. The resulting Centella asiatica extract has a better anti-inflammatory effect compared with other extraction methods.

[0017] More preferably, the preparation method of the peony root bark extract is as follows:

[0018] B1. Mix peony root bark powder with water, add cellulase, pectinase and amylase, and extract by ultrasonication to obtain a mixture.

[0019] B2. The mixture was fractionally extracted using ethanol and ethyl acetate. After extraction, the mixture was sequentially filtered, concentrated under vacuum, and freeze-dried to obtain the peony root bark extract.

[0020] The specific steps of the fractional extraction are as follows: Ethanol and ethyl acetate are added to the mixture, keeping the initial volume fraction of ethanol and ethyl acetate at 90%. Each ultrasonic treatment lasts for 10 minutes. Water is added to dilute the mixture, reducing the volume fraction of ethanol and ethyl acetate by 10%. After ultrasonic treatment for 60 minutes, the fractional extraction is completed.

[0021] By adopting the above technical solution, compared with solvent extraction of a single concentration, this application adopts a graded extraction method, that is, using solvents of different concentrations to extract the effective substances, so that the effective substances in the peony root bark extract are fully dissolved, thereby obtaining an extract with outstanding anti-inflammatory effects. Moreover, the preparation method provided by this application is easy to operate, easy to implement, and can be mass-produced.

[0022] This application also provides the use of an anti-inflammatory and repairing composition in cosmetics.

[0023] This application also provides a facial cleanser comprising the above-described anti-inflammatory and repairing composition, surfactant, and excipients.

[0024] Preferably, the anti-inflammatory and repairing composition accounts for 1-3% of the facial cleanser by mass.

[0025] Preferably, the surfactant includes stearic acid, palmitic acid, and sodium methyl cocoyl taurate.

[0026] Preferably, the excipients include at least one of humectants, antioxidants, emollients, and preservatives.

[0027] In summary, this application has the following beneficial effects:

[0028] The main anti-inflammatory components of Centella asiatica extract and Paeonia suffruticosa root bark extract are asiaticoside and paeonol, respectively, and their combination exhibits a good synergistic anti-inflammatory effect. In addition, Centella asiatica extract and Paeonia suffruticosa root bark extract also contain other ketones, phenols, and glycosides with anti-inflammatory effects. The composition provided in this application can promote the secretion of anti-inflammatory factors that repair the skin barrier by skin cells and inhibit the expression of inflammatory factors IL-1α and IL-6 to exert anti-inflammatory effects. The composition provided in this application uses a combination of Centella asiatica extract and Paeonia suffruticosa root bark extract to achieve anti-inflammatory and skin-repairing effects through different mechanisms of action. Furthermore, the combination of the two has a synergistic anti-inflammatory effect, showing promising application prospects in cosmetics. Detailed Implementation

[0029] The present application will be further described in detail below with reference to the embodiments.

[0030] Preparation Example

[0031] Preparation Example 1

[0032] A Centella asiatica extract is prepared by the following steps:

[0033] A1. Mix Centella asiatica powder with n-hexane at a mass ratio of 1:5, stir for 2 hours, filter, collect the filter residue and dry it to obtain defatted Centella asiatica powder.

[0034] A2. Mix defatted Centella asiatica powder and water at a ratio of 1:10. Based on the mass of the mixture, add 5% by weight cellulase, 3% by weight hemicellulase, and 2% by weight pectinase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained.

[0035] A3. Add alkaline protease to the mixture at an enzyme-to-solution mass ratio of 0.02:1, hydrolyze at 40°C for 3 hours, collect the supernatant by ultrafiltration, concentrate to 0.5 times the original volume at 45°C and 0.01 MPa vacuum, and freeze-dry at -55°C to obtain Centella asiatica extract.

[0036] Preparation Example 2

[0037] A Centella asiatica extract is prepared by the following steps:

[0038] A1. Crush Centella asiatica and add 5 times its weight of water. Reflux and extract 3 times at 40℃ for 0.5 hours each time. Filter the extract to obtain crude extract.

[0039] A2. Load the crude extract onto an XAD-10 macroporous resin column at a loading rate of 3 BV / h. Elute with an acid solution at pH 2 at a rate of 1 BV / h. Combine the eluent and the acid wash.

[0040] A3. Adjust the pH of the combined solution to neutral, filter, and load the filtrate onto an XAD-3 macroporous resin column at a loading rate of 0.5 BV / h. Elute with 30% ethanol at a rate of 2 BV / h. Monitor the elution with thin-layer chromatography, collect the ethanol eluent containing the target component, concentrate, and dry to obtain the final product.

[0041] Preparation Example 3

[0042] A Centella asiatica extract is prepared by the following steps:

[0043] Weigh 500g of Centella asiatica powder and soak it in 2500ml of 75% ethanol aqueous solution. Extract the extract four times using ultrasound at 50℃, one hour each time. Filter the extract and combine the filtrates. Concentrate under reduced pressure to obtain 20.5g of extract. Dissolve the extract in 200ml of water and extract three times with an equal volume of petroleum ether. Extract the lower phase aqueous solution four times with an equal volume of ethyl acetate. Collect the lower phase aqueous solution. Extract three times with an equal volume of n-butanol and ethyl acetate mixed solvent (1:1 volume ratio). Combine the upper phase extracts and decolorize the extract with activated carbon. Concentrate the decolorized extract under reduced pressure to obtain the final product.

[0044] Preparation Example 4

[0045] A peony root bark extract is prepared by the following steps:

[0046] B1. Mix peony root bark powder and water at a ratio of 1:10. Based on the mass of the mixture, add 4% by weight of cellulase, 6% by weight of pectinase, and 2% by weight of amylase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained.

[0047] B2. The mixture was fractionally extracted using ethanol and ethyl acetate. After extraction, the mixture was filtered and concentrated to obtain peony root bark extract.

[0048] B2. Add ethanol and ethyl acetate to the mixture, maintaining an initial volume fraction of ethanol and ethyl acetate of 90%. Sonicate at 40 kHz. Dilute with water every 10 minutes to reduce the volume fraction of ethanol and ethyl acetate by 10%. After sonication for 60 minutes, fractional extraction is completed. Filter and concentrate to 0.5 times the original volume at 45℃ and 0.01 MPa vacuum. Freeze-dry at -55℃ to obtain peony root bark extract.

[0049] Preparation Example 5

[0050] A peony root bark extract is prepared by the following steps:

[0051] B1. Mix peony root bark powder and water at a ratio of 1:10. Based on the mass of the mixture, add 4% by weight of cellulase, 6% by weight of pectinase, and 2% by weight of amylase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained.

[0052] B2. Add ethanol and ethyl acetate to the mixture, keeping the initial volume fraction of ethanol and ethyl acetate in the mixture at 90%. After ultrasonic treatment at 40 HKZ for 60 min, the extraction is complete. Filter the mixture and concentrate it to 0.5 times the original volume at 45℃ and 0.01 MPa vacuum. Freeze-dry at -55℃ to obtain the peony root bark extract.

[0053] Preparation Example 6

[0054] A peony root bark extract is prepared by the following steps:

[0055] B1. Mix peony root bark powder and water at a ratio of 1:10. Based on the mass of the mixture, add 4% by weight of cellulase, 6% by weight of pectinase, and 2% by weight of amylase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained.

[0056] B2. Add ethanol to the mixture, keeping the initial volume fraction of ethanol in the mixture at 90%. Sonicate at 40 kHz. Dilute with water every 10 minutes to reduce the volume fraction of ethanol by 10%. After sonication for 60 minutes, fractional extraction is completed. Filter and concentrate to 0.5 times the original volume at 45℃ and 0.01 MPa vacuum. Freeze-dry at -55℃ to obtain peony root bark extract.

[0057] Preparation Example 7

[0058] A peony root bark extract is prepared by the following steps:

[0059] B1. Mix peony root bark powder and water at a ratio of 1:10. Based on the mass of the mixture, add 4% by weight of cellulase, 6% by weight of pectinase, and 2% by weight of amylase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained.

[0060] B2. Add ethyl acetate to the mixture, maintaining an initial volume fraction of ethyl acetate of 90%. Sonicate at 40 kHz. Dilute with water every 10 minutes to reduce the volume fraction of ethyl acetate by 10%. After 60 minutes of sonication, fractional extraction is complete. Filter and concentrate to 0.5 times the original volume at 45°C and 0.01 MPa vacuum. Freeze-dry at -55°C to obtain peony root bark extract.

[0061] Example

[0062] Example 1

[0063] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0064] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0065] Examples 2-6

[0066] An anti-inflammatory and repairing composition is composed of a mixture of Centella asiatica extract and Paeonia suffruticosa root bark extract, the mass ratios of which are shown in Table 1.

[0067] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0068] Table 1. Components and their dosage ratios (total: 1000g)

[0069]

[0070]

[0071] Example 7

[0072] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0073] Centella asiatica extract was prepared in Preparation Example 2, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0074] Example 8

[0075] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0076] Centella asiatica extract was prepared in Preparation Example 3, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0077] Example 9

[0078] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0079] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia lactiflora root bark extract was prepared in Preparation Example 5.

[0080] Example 10

[0081] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0082] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia suffruticosa root bark extract was prepared in Preparation Example 6.

[0083] Example 11

[0084] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0085] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia suffruticosa root bark extract was prepared in Preparation Example 7.

[0086] Comparative Example

[0087] Comparative Example 1

[0088] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:0.4.

[0089] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0090] Comparative Example 2

[0091] An anti-inflammatory and repairing composition comprising Centella asiatica extract and Paeonia suffruticosa root bark extract in a mass ratio of 1:1.1.

[0092] Centella asiatica extract was prepared in Preparation Example 1, and Paeonia lactiflora root bark extract was prepared in Preparation Example 4.

[0093] Comparative Example 3

[0094] A Centella asiatica extract was prepared in Preparation Example 1.

[0095] Comparative Example 4

[0096] A peony root bark extract was prepared in Preparation Example 4.

[0097] Performance testing

[0098] The anti-inflammatory repair compositions prepared in the above embodiments and comparative examples were tested for anti-inflammatory effects at a concentration of 1%.

[0099] I. Inhibition test of pro-inflammatory factor IL-1α:

[0100] Test system: Raw264.7 macrophages (mouse macrophage line), purchased from the Cell Bank of the Chinese Academy of Sciences.

[0101] Reagents: High glucose DMEM culture medium (Gibco), fetal bovine serum (Sijiqing), PBS (Boster), MTT (Sigma), DMSO (Sigma), trypsin (GIBCO), LPS (Sigma), dexamethasone (National Institutes for Food and Drug Control), Mouse IL-1α kit (Abcam).

[0102] Test method: according to 6.4×10 5 Cells were seeded into 6-well plates at a seeding density of cells / well and incubated overnight in an incubator (37°C, 5% CO2). The compositions prepared according to the above examples and comparative examples (diluted to 1% by mass with water as solvent) were administered, and the results were detected using ELISA.

[0103] Drug administration: When the cell deposition rate in the 6-well plate reaches 40%-60%, the cells are administered to groups of 1.8 mL each, with 3 replicates per group. The cells are then incubated in an incubator (37℃, 5% CO2) for 2 hours.

[0104] LPS stimulation: After 2 hours of incubation, 200 μL of LPS working solution prepared from the corresponding test substance working solution was added to each well plate that had been drug-treated. The well plates were shaken left and right to mix the drug in the well plates. The final concentration of LPS was 1 μL / mL. The plates were then incubated in an incubator (37℃, 5% CO2) for another 22 hours.

[0105] Sample collection: After incubation, collect the cell culture supernatant into EP tubes. After collection, freeze the samples at -80°C.

[0106] IL-1α content detection: The Mouse IL-1α kit was used for detection.

[0107] The test results are recorded in Table 2 below.

[0108] Table 2 Results of the test for inhibition of pro-inflammatory factor IL-1α

[0109]

[0110]

[0111] Note: The significance of the negative group compared with the blank group is indicated by "#", and p < 0.01 is indicated by "##"; the significance of the positive group and the sample group compared with the blank group is indicated by "*", and p < 0.01 is indicated by "**".

[0112] As can be seen from the test results in Table 2:

[0113] Compared with the control group, the IL-1α secretion level in the negative group was significantly increased, indicating that the LPS stimulation condition was effective in this experiment.

[0114] Compared with the negative group, the IL-1α secretion level in the positive group was significantly reduced, indicating that this experiment was effective.

[0115] Compared with the negative control group, the IL-1α secretion in Examples 1-11 was significantly reduced, indicating that the scalp care essence prepared in this application has a good anti-inflammatory effect. The compositions in Examples 1-6, which are obtained by compounding Centella asiatica extract and Paeonia suffruticosa root bark extract prepared in Examples 1 and 4 respectively, have a better anti-inflammatory effect than Examples 7-11. Moreover, the anti-inflammatory effect is best when the compounding ratio of the two is 1:0.8, that is, Example 4 is the optimal example.

[0116] II. Concentration Test of the Composition: The anti-inflammatory repair composition prepared in Example 4 was used as the test sample. The IL-1α inhibition rate was tested at concentrations of 0.5%, 1%, 2%, 3%, and 4%, respectively. The test results are recorded in Table 3 below.

[0117] Table 3 Results of the test for inhibition of pro-inflammatory factor IL-1α

[0118]

[0119]

[0120] Note: The significance of the negative group compared with the blank group is indicated by "#", and p < 0.01 is indicated by "##"; the significance of the positive group and the sample group compared with the blank group is indicated by "*", and p < 0.01 is indicated by "**".

[0121] As can be seen from the test results in Table 3:

[0122] When the concentration of the anti-inflammatory and repairing composition is above 1%, its anti-inflammatory effect is significant. Considering the application cost, the optimal application concentration is 1-3%.

[0123] Application examples

[0124] Application Example 1

[0125] A facial cleanser, the components and their mass percentages are shown in Table 4, and its preparation steps are as follows:

[0126] 1) Premix 1: Put phase D into a clean stainless steel bucket, stir until dissolved and transparent, and set aside.

[0127] 2) Premix 2: Put phase C into a clean stainless steel bucket, stir until dissolved and free of particles, and set aside.

[0128] 3) Premix 3: Put phase F into a clean stainless steel bucket, stir until dissolved and free of particles, and set aside.

[0129] 4) Add phase A to the main pot, heat and stir. Heat to 80 degrees Celsius, stir with the inner and outer paddles at 500 rpm for 30 minutes, and confirm that it is completely dissolved and free of particles.

[0130] 5) Add the B-phase and C-phase premix 2 into the main pot, control the temperature at 80 degrees, and stir with the inner and outer paddles at 500 rpm for 5 minutes.

[0131] 6) Slowly add D-phase premix 1 into the main pot, stir with the inner and outer paddles at 500 rpm, control the temperature at 80 degrees, homogenize at 1000 rpm for 3 minutes.

[0132] 7) Start saponification for 45 minutes at 80 degrees Celsius, with the inner and outer paddles running at 500 rpm.

[0133] 8) After saponification for 45 minutes, cool down to 70 degrees Celsius, add E phase and F phase premix 3, stir with inner and outer paddles at 500 rpm, vacuum at -0.05 MPa, and continue to cool down.

[0134] 9) When the temperature drops to 55 degrees, add phase G, stir with the inner and outer impellers at 500 rpm, evacuate to -0.06 MPa, and continue cooling.

[0135] 10) When the temperature drops to 43 degrees, add the H and I phases, stir with the inner and outer impellers at 500 rpm, draw a vacuum of -0.07 MPa, continue to cool down, and when the temperature drops to 28-30 degrees, test the physicochemical properties and discharge the material.

[0136] in:

[0137] Phase A: 9, 6, 1, 10; Phase B: 3, 2; Phase C: 5, 12; Phase D: 5, 7, 5; Phase E: 4; Phase F: 5, 14; Phase G: 8; Phase H: 13, 11; Phase I: 15, 16, 17. The anti-inflammatory and repairing composition was prepared in Example 4.

[0138] Table 4. Components and dosages in Application Example 1

[0139]

[0140]

[0141]

[0142] The above description is merely a preferred embodiment of this application. The scope of protection of this application is not limited to the above embodiments. All technical solutions falling within the scope of this application's concept are within the scope of protection of this application. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principles of this application should also be considered within the scope of protection of this application.

Claims

1. An anti-inflammatory and repairing composition, characterized in that, It is composed of Centella asiatica extract and Paeonia suffruticosa root bark extract, wherein the mass ratio of Centella asiatica extract to Paeonia suffruticosa root bark extract is 1:(0.5-1); Centella asiatica extract was prepared by the following steps: A1. Mix Centella asiatica powder with n-hexane at a mass ratio of 1:5, stir for 2 hours, filter, collect the filter residue and dry it to obtain defatted Centella asiatica powder. A2. Mix defatted Centella asiatica powder and water at a ratio of 1:

10. Based on the mass of the mixture, add 5% by weight cellulase, 3% by weight hemicellulase, and 2% by weight pectinase. After ultrasonic extraction at 40℃ and 40KHz for 30 minutes, the mixture is obtained. A3. Add alkaline protease to the mixture at an enzyme-to-solution mass ratio of 0.02:1, hydrolyze at 40°C for 3 hours, collect the supernatant by ultrafiltration, concentrate to 0.5 times the original volume at 45°C and 0.01 MPa vacuum, and freeze-dry at -55°C to obtain Centella asiatica extract. Peony root bark extract was prepared by the following steps: B1. Mix peony root bark powder and water at a ratio of 1:

10. Add 4% by weight of cellulase, 6% by weight of pectinase, and 2% by weight of amylase based on the mass of the mixture. Extract the mixture by ultrasound at 40℃ and 40KHz for 30 minutes to obtain the final mixture. B2. The mixture was fractionally extracted using ethanol and ethyl acetate. After extraction, the mixture was filtered and concentrated to obtain peony root bark extract. B2. Add ethanol and ethyl acetate to the mixture, maintaining an initial volume fraction of ethanol and ethyl acetate of 90%. Sonicate at 40 kHz. Dilute with water every 10 minutes to reduce the volume fraction of ethanol and ethyl acetate by 10%. After sonication for 60 minutes, fractional extraction is completed. Filter and concentrate to 0.5 times the original volume at 45℃ and 0.01 MPa vacuum. Freeze-dry at -55℃ to obtain peony root bark extract.

2. The anti-inflammatory and repairing composition according to claim 1, characterized in that, The mass ratio of Centella asiatica extract to Paeonia suffruticosa root bark extract is 1:0.

8.

3. The use of the anti-inflammatory and repairing composition according to any one of claims 1-2 in the preparation of cosmetics.

4. A facial cleanser, characterized in that, Includes the anti-inflammatory repair composition, surfactant, and excipients as described in any one of claims 1-2.

5. The facial cleanser according to claim 4, characterized in that, The anti-inflammatory and repairing composition accounts for 1-3% of the mass of the facial cleanser.

6. The facial cleanser according to claim 4, characterized in that, The surfactants include stearic acid, palmitic acid, and sodium methyl cocoyl taurate.

7. The facial cleanser according to claim 4, characterized in that, The excipients include at least one of the following: humectant, antioxidant, emollient, and preservative.

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