A method for disinfecting rice seeds

Abstract

This invention relates to the field of rice cultivation technology and discloses a method for disinfecting rice seeds, comprising the following steps: disinfecting the seeds before hulling; removing the seed coat; and performing a secondary disinfection treatment on the surface of the embryo of the hulled seeds. Specifically, chlorine gas is used to disinfect the seeds before hulling; and a chlorine-containing disinfectant is used to disinfect the surface of the embryo, wherein the chlorine-containing disinfectant has an effective chlorine content of at least 6%. The technical solution of this application achieves rice seed disinfection by disinfecting the seeds before and after hulling, thoroughly eliminating bacteria and fungi on the seed coat and embryo surface, effectively ensuring that the seeds are in a sterile state.

Description

Technical Field

[0001] This invention relates to the field of rice cultivation technology, specifically to a method for disinfecting rice seeds. Background Technology

[0002] Clean, uncontaminated, and highly viable rice seeds are essential materials for rice genetic transformation. Currently, the conventional disinfection process for mature rice seeds typically involves treatment with 75% alcohol and a low concentration of mercuric chloride, followed by rinsing with sterile water. However, due to the environmental pollution caused by mercuric chloride and the cumbersome nature of subsequent treatments, this method is gradually being phased out. Furthermore, rice seeds harbor a significant number of bacteria and fungi, which cannot be completely eliminated by existing disinfection methods, resulting in unsatisfactory disinfection effects. Summary of the Invention

[0003] This application provides a method for disinfecting rice seeds to solve the aforementioned technical problem of poor disinfection effect in existing technologies.

[0004] According to one aspect of this application, one embodiment provides a method for disinfecting rice seeds, comprising the following steps:

[0005] S1. Disinfect the seeds before shelling;

[0006] S2. Peel the seeds;

[0007] S3. Perform a secondary disinfection treatment on the surface of the embryo of the peeled seeds.

[0008] In one embodiment, in step S1, chlorine gas is used to disinfect the seeds before shelling.

[0009] In one embodiment, in step S1, the seeds before hulling are selected from mature rice seeds that are large, plump, and free of disease spots.

[0010] In one embodiment, in step S2, after the seeds are peeled, seeds with intact embryos, no disease spots on the surface, and bright color are selected for the secondary disinfection treatment.

[0011] In one embodiment, in step S3, a chlorine-containing disinfectant is used to disinfect the surface of the embryo, wherein the effective chlorine content of the chlorine-containing disinfectant is at least 6%.

[0012] In one embodiment, step S3 includes the following steps:

[0013] S31. Disinfect the surface of the embryo of peeled seeds with a chlorine-containing disinfectant, and then discard the chlorine-containing disinfectant;

[0014] S32. After the peeled seeds are washed with sterile water to remove residue, they are soaked in fresh sterile water for a set time.

[0015] In one embodiment, step S3 further includes the following steps:

[0016] S33. Clean the peeled seeds with alcohol to remove the disinfection residue from step S1; then discard the alcohol.

[0017] S34. Wash the peeled seeds with sterile water to remove surface alcohol and impurities.

[0018] In one embodiment, the chlorine-containing disinfectant includes 84 disinfectant or sodium hypochlorite.

[0019] In one embodiment, the method for disinfecting rice seeds further includes the following steps:

[0020] S4. Drying the peeled seeds.

[0021] The technical solution of the above embodiments of this application adopts the method of disinfecting rice seeds before and after dehulling to achieve disinfection treatment. The bacteria and fungi on the seed coat and embryo surface are thoroughly eliminated, which can effectively ensure that the seeds are in a sterile state. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of an apparatus for chlorine disinfection of rice seeds in one embodiment.

[0023] Figure 2 The columnar samples obtained in the examples show the effects of secondary disinfection with chlorine and sodium hypochlorite, and disinfection with sodium hypochlorite / 84 disinfectant on seed contamination rate and callus induction rate. Detailed Implementation

[0024] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0025] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are merely some, not all, of the embodiments of the present application. All other embodiments obtained by those skilled in the art based on the embodiments of the present application without creative effort should fall within the scope of protection of the present application.

[0026] It should be noted that the terms "first," "second," etc., used in this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0027] It should be understood that when an element (such as a layer, film, region, or substrate) is described as being "on" another element, the element may be directly on the other element, or there may be an intermediate element present. Moreover, in this application, when an element is described as being "connected" to another element, the element may be "directly connected" to the other element, or "connected" to the other element via a third element.

[0028] Example 1

[0029] This embodiment provides a method for disinfecting rice seeds, including the following steps:

[0030] S1. Disinfect the seeds before shelling;

[0031] S2. Peel the seeds;

[0032] S3. Perform a secondary disinfection treatment on the surface of the embryo of the peeled seeds;

[0033] S4. Drying the peeled seeds.

[0034] In one embodiment, in step S1, chlorine gas is used to disinfect the seeds before shelling. Disinfection with chlorine gas (100% effective chlorine content) can effectively kill contaminants on the seed surface without affecting seed viability.

[0035] Regarding the use of chlorine gas to disinfect the surface of mature rice seed coats, the following specific operating steps are provided: 1. Select large, plump, and disease-free mature rice seeds, and wrap them in appropriately sized aluminum foil; make numerous small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the foil, effectively killing surface pathogens; then place the seeds in a container to await disinfection; 2. [The text abruptly ends here, likely due to an incomplete sentence or missing information.] Figure 1 Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0036] In one embodiment, in step S2, after the seeds are peeled, seeds with intact embryos, no disease spots on the surface, and a bright color are selected for the secondary disinfection treatment. That is, the secondary disinfection treatment is carried out after the above-mentioned chlorine disinfection treatment.

[0037] In one embodiment, in step S3, a chlorine-containing disinfectant is used to disinfect the surface of the embryo, wherein the effective chlorine content of the chlorine-containing disinfectant is at least 6%. Preferably, the chlorine-containing disinfectant includes 84 disinfectant or sodium hypochlorite.

[0038] In one embodiment, step S3 includes the following steps:

[0039] S33. Use alcohol to clean the peeled seeds to remove the disinfection residue from step S1; then pour out the alcohol; wherein, the volume fraction of the alcohol is preferably 75%; pour the alcohol into the container containing the peeled seeds and shake or agitate for 1-2 minutes.

[0040] S34. Wash the peeled seeds with sterile water to remove surface alcohol and impurities; usually, wash 2-3 times to ensure that residues are removed.

[0041] S31. Disinfect the surface of the seed embryo of the peeled seeds with a chlorine-containing disinfectant, and then discard the chlorine-containing disinfectant; in specific operation, disinfect the inner wall of the container and the seed surface with sodium hypochlorite or 80% 84 disinfectant solution for 4-5 minutes; then replace with fresh sodium hypochlorite or 80% 84 disinfectant solution for 15 minutes; and place on a shaker for shaking at 28℃ and 140r / min.

[0042] S32. After the peeled seeds are washed with sterile water (generally 5-7 times) to remove residue, they are soaked in fresh sterile water and left to stand for 1-2 hours. The drying process for the peeled seeds is as follows: place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0043] The rice seed disinfection method of this application involves first disinfecting mature rice seeds with husks using chlorine gas, then removing the husks, and finally disinfecting the embryo surface using conventional 84 disinfectant or sodium hypochlorite. This method significantly reduces the contamination rate of mature seeds and does not affect the induction of callus tissue in the embryo. While 84 disinfectant and sodium hypochlorite can effectively disinfect most mature seeds, a small number are difficult to clean completely with these two disinfectants. This is because the environment during the rice seed growth period is complex, resulting in a large number of bacteria and fungi present on the seed surface. During manual husk removal, many bacteria and fungi are transferred to the embryo surface. Treating the embryo with 84 disinfectant or sodium hypochlorite can only remove most of these bacteria and fungi, leaving a small number remaining on the embryo surface. After disinfection, when inoculated onto a culture medium, these bacteria and fungi absorb nutrients from the medium and proliferate extensively. However, the rice seed disinfection method of this application, by disinfecting the seeds with chlorine gas before husk removal, overcomes the drawback of incomplete embryo treatment with 84 disinfectant or sodium hypochlorite.

[0044] Research and experiments have shown that specific treatment methods can effectively ensure seeds remain sterile, primarily involving disinfection before and after shelling. Disinfection must be performed at least once. The seeds can be grown in any environment, including complex environments.

[0045] The following experiments illustrate the advantages of this rice seed disinfection method.

[0046] Example 2

[0047] Rice seeds using R021 are treated with the disinfection method of this invention, including the following steps:

[0048] Step 1: Disinfect the surface of R021 mature rice seed coat with chlorine gas.

[0049] 1. Select large, plump, and disease-free mature rice seeds, and wrap them in aluminum foil of appropriate size; make many small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the aluminum foil, effectively killing the surface pathogens; and place the seeds in a container to be sterilized.

[0050] 2. In such Figure 1 Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0051] Step 2: Disinfect the surface of the embryo of mature R021 seeds with sodium hypochlorite.

[0052] 1. After chlorine disinfection, remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and bright color for the following disinfection treatment;

[0053] 2. Treat the seeds with 75% alcohol for 1-2 minutes, shaking or agitating them;

[0054] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0055] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite disinfectant for 4-5 minutes, then replace with fresh sodium hypochlorite disinfectant and disinfect for 15 minutes; and shake on a shaker at 28℃ and 140r / min.

[0056] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0057] 6. Soak in fresh sterile water for 1-2 hours;

[0058] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0059] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0060] Example 3

[0061] Rice seeds using R024 are treated with the disinfection method of this invention, including the following steps:

[0062] Step 1: Disinfect the surface of R024 mature rice seed coat with chlorine gas.

[0063] 1. Select large, plump, and disease-free mature rice seeds, and wrap them in aluminum foil of appropriate size; make many small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the aluminum foil, effectively killing the surface pathogens; and place the seeds in a container to be sterilized.

[0064] 2. In such Figure 1 Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0065] Step 2: Disinfect the surface of the embryo of mature R024 seeds with sodium hypochlorite.

[0066] 1. After chlorine disinfection, remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and bright color for the following disinfection treatment;

[0067] 2. Treat the seeds with 75% alcohol for 1-2 minutes, shaking or agitating them;

[0068] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0069] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite for 4-5 minutes, then replace with fresh sodium hypochlorite disinfectant and disinfect for 15 minutes, and shake on a shaker at 28℃ and 140r / min.

[0070] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0071] 6. Soak in fresh sterile water for 1-2 hours;

[0072] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0073] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0074] Example 4

[0075] Rice seeds using R037 are treated with the disinfection method of this invention, including the following steps:

[0076] Step 1: Disinfect the surface of mature rice seed coats (R037) with chlorine gas.

[0077] 1. Select large, plump, and disease-free mature rice seeds, and wrap them in aluminum foil of appropriate size; make many small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the aluminum foil, effectively killing the surface pathogens; and place the seeds in a container to be sterilized.

[0078] 2. In such Figure 1 Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0079] Step 2: Disinfect the surface of the embryo of mature R037 seeds with sodium hypochlorite.

[0080] 1. After chlorine disinfection, remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and bright color for the following disinfection treatment;

[0081] 2. Treat the seeds with 75% alcohol for 1-2 minutes, shaking or agitating them;

[0082] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0083] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite disinfectant for 4-5 minutes, then replace with fresh sodium hypochlorite disinfectant and disinfect for 15 minutes; and shake on a shaker at 28℃ and 140r / min.

[0084] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0085] 6. Soak in fresh sterile water for 1-2 hours;

[0086] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0087] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0088] Example 5

[0089] Rice seeds using R038 are treated with the disinfection method of this invention, including the following steps:

[0090] Step 1: Disinfect the surface of mature rice seed coat R038 with chlorine gas.

[0091] 1. Select large, plump, and disease-free mature rice seeds, and wrap them in aluminum foil of appropriate size; make many small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the aluminum foil, effectively killing the surface pathogens; and place the seeds in a container to be sterilized.

[0092] 2. In such Figure 1 Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0093] Step 2: Disinfect the surface of the embryo of mature R038 seeds using sodium hypochlorite disinfectant solution.

[0094] 1. After chlorine disinfection, remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and bright color for the following disinfection treatment;

[0095] 2. Treat the seeds with 75% alcohol for 1-2 minutes, shaking or agitating them;

[0096] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0097] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite or 80% 84 disinfectant for 4-5 minutes, then replace with fresh sodium hypochlorite or 80% 84 disinfectant and disinfect for 15 minutes; and shake on a shaker at 28℃ and 140r / min.

[0098] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0099] 6. Soak in fresh sterile water for 1-2 hours;

[0100] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0101] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0102] Example 6

[0103] Rice seeds using R040 are treated with the disinfection method of this invention, including the following steps:

[0104] Step 1: Disinfect the surface of R040 mature rice seed coat with chlorine gas.

[0105] 1. Select large, plump, and disease-free mature rice seeds, and wrap them in aluminum foil of appropriate size; make many small holes in the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in the aluminum foil, effectively killing the surface pathogens; and place the seeds in a container to be sterilized.

[0106] 2. In such Figure 1Sodium hypochlorite is added to the conical flask on the left side of the apparatus shown, and 30% concentrated hydrochloric acid is added to the container above, with a sodium hypochlorite to concentrated hydrochloric acid ratio of 3:1. The generated chlorine gas is used to disinfect the seeds for 1-2 hours. Excess chlorine gas is treated with 0.1M sodium hydroxide to prevent it from entering the air and polluting the environment.

[0107] Step 2: Disinfect the surface of the R040 mature seed embryos with sodium hypochlorite disinfectant solution.

[0108] 1. After chlorine disinfection, remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and bright color for the following disinfection treatment;

[0109] 2. Treat the seeds with 75% alcohol for 1-2 minutes, shaking or agitating them;

[0110] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0111] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite disinfectant for 4-5 minutes, then replace with fresh sodium hypochlorite or 80% 84 disinfectant for 15 minutes; and shake on a shaker at 28℃ and 140r / min.

[0112] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0113] 6. Soak in fresh sterile water for 1-2 hours;

[0114] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 2 hours.

[0115] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0116] The results of the above five embodiments after 7 days are shown in Table 1. The contamination rate is calculated on a per-plate basis. That is, whether 1 or 20 seeds in 1 plate are contaminated, it is considered as 1.

[0117] Example 7

[0118] For the rice varieties involved in Examples 2-6 above, sodium hypochlorite or 84 disinfectant was used as a control group for disinfection treatment. The specific steps of the disinfection method are as follows:

[0119] 1. Remove the seed coat; select seeds with intact embryos, no disease spots on the surface, and a bright color for the following disinfection treatment;

[0120] 2. Treat the seeds with 75% alcohol for 2 minutes, shaking or agitating them;

[0121] 3. Wash the seeds 2-3 times with sterile water to remove surface alcohol and other impurities;

[0122] 4. Disinfect the inner wall of the disinfection container and the surface of the seeds with sodium hypochlorite or 80% 84 disinfectant for 4-5 minutes, then replace with fresh sodium hypochlorite or 84 disinfectant and disinfect for 20 minutes; and shake on a shaker at 28℃ and 140r / min.

[0123] 5. Pour out the disinfectant on the sterile workbench and rinse with sterile water 5-7 times;

[0124] 6. Soak in fresh sterile water for 1-2 hours;

[0125] 7. Place 4-5 layers of sterile filter paper in a sterile petri dish; pour the soaked seeds onto the filter paper and blow dry the surface moisture, which takes about 1-2 hours.

[0126] 8. After drying, the seeds were inoculated onto the appropriate culture medium. After 7 days, the contamination rate and the induction rate of primary callus were calculated. Ten dishes were inoculated, with 20 seeds in each dish.

[0127] In this control group, the results of the five varieties of seeds after 7 days of inoculation are shown in Table 1. The contamination rate is calculated on a per-plate basis, that is, 1 is considered as 1 regardless of whether 1 or 20 seeds in 20 seeds in 1 plate are contaminated.

[0128] Wherein, contamination rate = number of dishes containing contaminated seeds / total number of inoculated dishes * 100%; callus induction rate is the primary callus induction rate after 7 days, callus induction rate = number of seeds that grow callus tissue / total number of inoculated seeds (excluding callus tissue in contaminated dishes) * 100%.

[0129] A comparison was made between the above five varieties of seeds using a two-stage disinfection method with chlorine and sodium hypochlorite, and a disinfection method using sodium hypochlorite or 84 disinfectant (Table 1 and 2). Figure 1 After secondary disinfection using chlorine and sodium hypochlorite, the contamination rate of seeds from five varieties significantly decreased after 7 days of induction. Specifically, the contamination rate of R021, R037, and R040 was 0%, while the contamination rate of R024 and R038 decreased to 20%. Furthermore, after chlorine disinfection, the callus induction rate of the seed embryos, after being cultured on a callus induction medium, exceeded 50% for all five varieties. The final results indicate that the contamination rate of rice seeds disinfected using a two-stage method with chlorine and sodium hypochlorite was lower than that of seeds disinfected using only sodium hypochlorite or 84 disinfectant. This demonstrates that the two-stage disinfection method with chlorine and sodium hypochlorite is suitable for the disinfection of these five severely contaminated rice seeds.

[0130] This also includes a blank group, in which the seeds of the five varieties are not disinfected, and the contamination rate of the seeds is 100%.

[0131] In summary, the rice seed disinfection method of this application adopts a treatment approach of disinfecting the seeds before and after hulling, which makes the elimination of bacteria and fungi in the seeds more thorough.

[0132] Table 1. Effects of secondary disinfection with chlorine and sodium hypochlorite, and disinfection with sodium hypochlorite / 84 disinfectant, on seed contamination rate and callus induction rate.

[0133]

[0134] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A method for disinfecting rice seeds, characterized in that, Includes the following steps: S1. Disinfect the seeds before shelling with chlorine gas; wherein, wrap the seeds in aluminum foil of appropriate size, and make many small holes in the surface of the aluminum foil with a scalpel to allow a small amount of chlorine gas to enter the seeds wrapped in aluminum foil, effectively killing the surface bacteria; the chlorine gas used in step S1 is produced by adding 30% concentrated hydrochloric acid to sodium hypochlorite, the ratio of sodium hypochlorite to concentrated hydrochloric acid is 3:1, and the chlorine gas produced disinfects the seeds for 1-2 hours; S2. Peel the seeds; S3. The surface of the seed embryo of the peeled seeds is disinfected a second time using a chlorine-containing disinfectant; wherein the effective chlorine content of the chlorine-containing disinfectant is at least 6%; the chlorine-containing disinfectant includes 84 disinfectant or sodium hypochlorite.

2. The method for disinfecting rice seeds according to claim 1, characterized in that, In step S1, the seeds selected before hulling are mature rice seeds that are large, plump, and free of disease spots.

3. The method for disinfecting rice seeds according to claim 1, characterized in that, In step S2, after the seeds are peeled, seeds with intact embryos, no disease spots on the surface, and bright color are selected for the secondary disinfection treatment.

4. The method for disinfecting rice seeds according to claim 1, characterized in that, Step S3 includes the following steps: S31. Disinfect the surface of the embryo of peeled seeds with a chlorine-containing disinfectant, and then discard the chlorine-containing disinfectant; S32. After the peeled seeds are washed with sterile water to remove residue, they are soaked in fresh sterile water for a set time.

5. The method for disinfecting rice seeds according to claim 4, characterized in that, Step S3 also includes the following steps: S33. Clean the peeled seeds with alcohol to remove the disinfection residue from step S1; then discard the alcohol. S34. Wash the peeled seeds with sterile water to remove surface alcohol and impurities.

6. A method for disinfecting rice seeds according to any one of claims 1-5, characterized in that, The method for disinfecting rice seeds also includes the following steps: S4. Drying the peeled seeds.

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