A method for promoting population regeneration of an endangered plant, malania oleifera

Abstract

The application discloses a method for promoting population renewal of endangered plant Melia toosendan, which comprises the following steps: obtaining a Melia toosendan population with poor ecological resistance and low plant reserves by investigating forestry data of previous years; performing field investigation on seedlings, saplings and seedling survival rate of the Melia toosendan population to screen out a Melia toosendan population to be intervened; opening a transplant pit at the best growth range of seedlings of a non-flowering and fruiting plant of the Melia toosendan population to be intervened; placing a degradable waterproof bag in the transplant pit; and transplanting and domesticating a Melia toosendan seedling obtained by domestication after injecting nutrient solution into the waterproof bag. The method transplants and domesticates a Melia toosendan seedling for a small Melia toosendan population with low plant reserves and low renewal capacity, and uses nutrient solution and a degradable waterproof bag to improve the survival rate of the transplanted Melia toosendan seedling, so that the number of seedling reserves of the small Melia toosendan population is increased, and the population renewal capacity of the small Melia toosendan population is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant protection, in particular to a method for promoting population renewal of endangered plant Allium fistulosum. BACKGROUND

[0002] Allium fistulosum is a single-species genus of tall evergreen trees in the Alliaceae family. Allium fistulosum seeds contain high nervonic acid, which has high economic and medical value.

[0003] Through research on Allium fistulosum growing in the field, many Allium fistulosum populations have poor renewal ability. These Allium fistulosum populations with poor renewal ability have the same characteristics: there are many mature flowering and fruiting plants, but there are few seedlings and saplings under the mother tree, and natural population renewal is difficult. Through long-term investigation and observation of wild Allium fistulosum populations, it is observed that flowering and fruiting plants can form a large number of fruits during the fruiting period, and a large number of fruits mature and fall off during the fruit maturation period. However, only a small number of seedlings and saplings are sporadically distributed under the mother tree. First, although Allium fistulosum can produce a large number of seeds, due to various factors, only a small number of Allium fistulosum seeds can germinate. Second, the germinated Allium fistulosum is difficult to survive the seedling stage and grow into a sapling due to its own and external factors. Through the above description, the low seed germination rate and low seedling survival rate of wild Allium fistulosum are the direct causes of the difficulty in population renewal. In order to save the endangered Allium fistulosum, artificial intervention is needed to promote the population renewal of wild Allium fistulosum.

[0004] Under the condition of limited artificial intervention cost budget and staff energy, and the large number of Allium fistulosum populations and the long distance between Allium fistulosum populations, it is necessary to seek an effective method to screen out Allium fistulosum populations to be intervened.

[0005] The artificial intervention method for promoting population renewal of wild Allium fistulosum of the present application is to artificially transplant and domesticate cold-tolerant Allium fistulosum seedlings under the unflowering and fruiting plants of Allium fistulosum populations with poor renewal ability.

[0006] The information disclosed in this BACKGROUND section is only intended to increase an understanding of the general context in which the application can be practiced. It is not admitted that any of the information provided in this section constitutes prior art. SUMMARY

[0007] The purpose of the present application is to provide a method for promoting population renewal of endangered plant Allium fistulosum, which screens out Allium fistulosum populations to be protected, and transplants and domesticates artificial cultivation seedlings, aiming to enhance the population renewal ability of Allium fistulosum populations in urgent need of intervention and improve the ecological resistance of Allium fistulosum populations.

[0008] To achieve the above object, the application provides a method for promoting population renewal of endangered plant Alliariapetiolata, comprising the following steps:

[0009] S1, investigating and obtaining the Alliariapetiolata population to be intervened;

[0010] S1.1, arranging the forestry data of each Alliariapetiolata population in previous years, and screening the Alliariapetiolata population with less plant reserves according to the plant reserves;

[0011] S1.2, taking the Alliariapetiolata population with less plant reserves as the investigation object to perform field investigation, and obtaining the Alliariapetiolata population to be intervened;

[0012] S1.3, marking the non-flowering and fruiting plants in the Alliariapetiolata population to be intervened;

[0013] S2, determining the optimal growth range for population renewal;

[0014] S3, domesticating Alliariapetiolata seedlings;

[0015] S4, determining the transplanting position according to the optimal growth range for population renewal, digging a transplanting pit at the transplanting position, placing a degradable waterproof bag in the transplanting pit, arranging a nutrient pool at the bottom of the degradable waterproof bag, filling the nutrient pool with small stones, injecting nutrient solution, vertically placing the Alliariapetiolata seedlings in the center of the waterproof bag, and immersing part of the root system in the nutrient solution, and then backfilling sand and original soil in sequence.

[0016] According to the above technical solution, the method screens the small Alliariapetiolata population from the forestry data by taking the plant reserves as the condition, overcomes the defect that the small Alliariapetiolata population with low ecological resistance cannot be protected in time and declines or even disappears due to the lack of sequence in population protection, and achieves the effect of promoting the benign growth of the small Alliariapetiolata population. Further, the method performs field investigation on the population renewal of the Alliariapetiolata population based on the small Alliariapetiolata population with less plant reserves, overcomes the defects of multiple screening objects, wide range and long time consumption of the Alliariapetiolata population, and achieves the effects of reducing the range of field investigation and reducing the workload of investigation. Meanwhile, the method selects the artificial intervention object in a targeted manner, and avoids the decline or even disappearance of the Alliariapetiolata population with poor population renewal ability in the small Alliariapetiolata population. Through the steps of arrangement, screening, investigation and marking in the method, the purpose is to screen the Alliariapetiolata population to be protected, and to protect the Alliariapetiolata population with low ecological resistance and weak renewal ability in a timely manner by artificial intervention with limited efforts and resources. The domestication and retransplantation of the Alliariapetiolata seedlings improve the field adaptation ability of the Alliariapetiolata seedlings and improve the success rate of transplantation. When transplanting, the nutrient solution is injected at the bottom of the degradable waterproof bag to reduce the workload of applying nutrient solution, promote the downward growth of the root system of the Alliariapetiolata seedlings, realize deep rooting of the root system, form a relatively closed environment in the degradable waterproof bag, reduce the temperature change in the degradable waterproof bag, and prevent the Alliariapetiolata seedlings from being harmed by large temperature difference.

[0017] Preferably, in the technical scheme, the degradable waterproof bag is composed of acrylic acid, an adhesive and a beauty bag, the waterproof material made of acrylic acid and propylene glycol is sprayed on the inside and outside of the beauty bag, and is dried after being sprayed n times, wherein n>1.

[0018] Preferably, in the technical scheme, the adhesive comprises one or more of propylene glycol, sodium dodecyl sulfate and polyacrylamide.

[0019] According to the technical scheme, the beauty bag has the characteristics of cheap source, suitable degradation speed and no harm, and the preparation principle of the degradable waterproof bag is that the easily degradable beauty bag is used as the bag body of the degradable waterproof bag, and multiple layers of waterproof paint are sprayed on the inside and outside of the beauty bag to obtain waterproof effect. The waterproof paint is made of acrylic acid, sodium dodecyl sulfate and polyacrylamide, and the principle is that sodium dodecyl sulfate and the surface hydroxyl or proton of acrylic acid are coupled to form a monolayer through a chemical reaction, tightly connecting the acrylic acid molecules and the polyacrylamide molecules, effectively changing the interface state between the acrylic acid, the polyacrylamide and the beauty bag, the thickening property and the adhesion of the polyacrylamide, increasing the adhesion of the acrylic acid and the beauty bag, and thus reducing the probability of falling off of the waterproof film.

[0020] Preferably, in the technical scheme, the nutrient solution comprises triacontanol, oligogalacturonide oligosaccharide and fumaric acid.

[0021] Preferably, in the technical scheme, the nutrient solution further comprises potassium dihydrogen phosphate and zeatin.

[0022] According to the technical scheme, the nutrient solution is composed of 5-15% triacontanol, 30-50% oligogalacturonide oligosaccharide, 0.5-5% fumaric acid, 30-50% potassium dihydrogen phosphate and 5-15% zeatin in terms of mass percentage, the oligogalacturonide oligosaccharide improves the permeability of root hair cells of seedlings, improves the intensity of the seedling root hair in absorbing the nutrient solution, the potassium dihydrogen phosphate maintains the K concentration inside and outside the root hair cells, maintains the normal physiological process of water absorption of the plant root system, and prevents physiological drought of the root system. The nutrient solution enters the seedling plant together to promote the elongation and robustness of the seedling root system and the luxuriant branches and leaves.

[0023] Preferably, in the technical scheme, in the step S1, the field investigation specifically comprises the following steps:

[0024] S3.1, the first domestication stage: the garlic fruit seedlings are sequentially subjected to normal temperature, variable temperature and low temperature treatment to obtain cold-resistant garlic fruit seedlings;

[0025] S3.2, the second acclimation stage: a simple cultivation shed is set up in the population of garlic fruit to be intervened, the cold-resistant garlic fruit seedlings are moved to the simple cultivation shed, and the seedlings are acclimated by opening the top at a fixed time until the garlic fruit seedlings adapt to the climate of the population of garlic fruit to be intervened, and the simple cultivation shed is removed.

[0026] Preferably, in the above technical solution, in step S3.1, the variable temperature is between the normal temperature and the low temperature, the normal temperature is 28-32℃, and the low temperature is 7-10℃.

[0027] Preferably, in the above technical solution, the variable temperature amplitude is 2-8℃.

[0028] According to the above technical solution, the seedlings are acclimated by a large variable temperature of 18-32℃, and the variable temperature amplitude can be 4-8℃. The principle is that the temperature change in the range of 18-32℃ will not cause cell freezing injury to damage the root system and leaves of the seedlings, at the same time, the temperature change is simulated to be changeable in the wild, the adaptability of cells to temperature change is improved, and the survival rate of transplanting is improved; the variable temperature is small, that is, 7-18℃, and the temperature is gradually lowered by 2-3℃ each time, and the temperature in the range of 4-18℃ reaches a certain critical value, which is likely to cause freezing injury of leaves and roots of the seedlings, causing irreversible damage to the seedlings. Therefore, the variable temperature should be slowly lowered, and the temperature should be maintained for a preset time after each temperature lowering, and whether the seedlings have leaf freezing injury is observed. If there is no freezing injury, the temperature lowering continues until the cold-resistant garlic fruit seedlings are obtained.

[0029] Preferably, in the above technical solution, in step S1.2, the population of garlic fruit with less plant reserves is taken as the investigation object for field investigation, and the specific steps for obtaining the population of garlic fruit to be intervened are as follows:

[0030] S1.2.1, the number of seedlings and saplings in the population is investigated in the field by taking the population of garlic fruit with less plant reserves as the investigation object;

[0031] S1.2.2, the seed germination of the population of garlic fruit with less plant reserves is recorded in the seed germination period, and the germinated seeds are marked;

[0032] S1.2.3, the seedling rate is calculated by recording the seedling of the germinated seeds in the seedling period;

[0033] S1.2.4, the population of garlic fruit to be intervened is screened from the population of garlic fruit with less plant reserves by taking the number of seedlings, the number of saplings and the seedling rate as indexes.

[0034] According to the technical scheme, when the garlic fruit population with less plant reserves is investigated in the field, the population renewal of the garlic fruit population is understood through the number of seedlings and saplings, and the seed germination and seedling growth are observed in the seed germination period and the seedling growth period, so that the population renewal of the garlic fruit population is directly obtained, the garlic fruit population with weak renewal capacity is screened out as the garlic fruit population to be intervened by taking the number of seedlings and saplings as indexes, the accuracy of the artificial intervention object is improved, the garlic fruit population renewal capacity is timely protected, and the expansion of the garlic fruit population is promoted.

[0035] Preferably, in the technical scheme, the determining the population renewal growth range in the step S2 specifically comprises the following steps:

[0036] S2.1, taking the trunk of the flowering and fruiting plant as the center, the area 2 m away from the trunk to the crown cover is divided into annular samples, and the number, height and ground diameter of the seedlings and saplings in each annular sample are recorded as the population renewal data;

[0037] S2.2, the population renewal data is processed by using the SPSS 19.0 software, the position range of the population renewal is obtained, and the transplanting position of the garlic fruit seedling is determined.

[0038] Compared with the prior art, the present application has the following beneficial effects:

[0039] The principle of improving the population renewal capacity of the garlic fruit population is that: (1) the garlic fruit small population with low ecological resistance is screened out by taking the plant reserves, and then the garlic fruit population to be intervened is determined in the garlic fruit small population, so that the problem of determining the garlic fruit population to be intervened is overcome, the screening time in the early stage is effectively shortened, and the screening workload is reduced; (2) the degradable waterproof bag made of the waterproof coating sprayed on the beauty bag is provided, the bottom is provided as a nutrient pool to promote the root system of the garlic fruit seedling to grow downward and realize deep rooting, so that the growth vigor of the seedling is improved and the tree body is prevented from falling; (3) fumaric acid and potassium dihydrogen phosphate in the nutrient solution promote the horizontal and vertical growth of the root system under the condition of maintaining the dynamic constant pressure of the root system, corn element, potassium dihydrogen phosphate, oligogalacturonide oligosaccharide, triacontanol and fumaric acid jointly promote the vegetative growth of the garlic fruit seedling and improve the self resistance. BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 is a diagram of the garlic fruit sapling transplanted for 2 years in a specific embodiment of the present application;

[0041] Figure 2 is a diagram of the garlic fruit sapling transplanted for 2 years in a specific embodiment of the present application;

[0042] Figure 3 is a diagram of the garlic fruit sapling transplanted for 3 years in a specific embodiment of the present application;

[0043] Figure 4 is a diagram of the seedling of Malania oleifera small population in the field survey in a specific embodiment of the present application;

[0044] Figure 5 is a diagram of the sapling of Malania oleifera small population in the field survey in a specific embodiment of the present application. DETAILED DESCRIPTION

[0045] The specific embodiments of the present application are described in detail below with specific embodiments, but it should be understood that the protection scope of the present application is not limited by the specific embodiments. Example 1

[0046] A method for promoting the regeneration of an endangered plant Malania oleifera population, the method comprising the following steps:

[0047] S1, carrying out investigation, identifying the Malania oleifera population to be intervened, and marking the unflowering and fruiting plants in the Malania oleifera population to be intervened;

[0048] Further, step S1 is specifically:

[0049] S1.1, collating the previous forestry data of the Malania oleifera population in Guangxi Zhuang Autonomous Region, sorting the Malania oleifera population according to the plant reserves, screening out 6 Malania oleifera small populations with less plant reserves, investigating the location, land area and climate of the Malania oleifera small population, and laying a foundation for field investigation;

[0050] S1.2, taking the Malania oleifera small population with less plant reserves as the investigation object, and carrying out field investigation on one Malania oleifera small population to obtain the Malania oleifera population to be intervened; wherein the specific steps of field investigation are:

[0051] S1.2.1, taking one Malania oleifera small population with less plant reserves as the investigation object, and field investigating the total number of seedlings and saplings in the population to be 18, the land area of the Malania oleifera small population is 121m 2 , according to the formula seedling and sapling number = total number of seedlings and saplings divided by land area and multiplied by 64m 2 , i.e. seedling and sapling number = 18 ÷ 121m 2 × 64m 2 , the number of seedlings and saplings of the Malania oleifera small population is obtained as 10 / 64m 2 ;

[0052] S1.2.2, recording the germination of the Malania oleifera population in the seed germination period, marking the position of the germinated seeds with eye-catching signboards, and observing the seedling situation after 1 year, the number of marked germinated seeds is 54 / 64m 2 ;

[0053] S1.2.3, the seedling stage is one year, and the seedling situation of the marked germinated seeds is recorded at the end of the seedling stage, and the seedling number is 17 per 64 m 2 According to the seedling rate formula: seedling rate=(seedling number / germinated seeds)*100%, i.e., seedling rate=(17÷54)×100%, the seedling rate is calculated to be 31.48%;

[0054] S1.2.4, because the number of seedlings and young trees of the Allium fistulosum small population is too small, the seedling rate is lower than 60%, so the Allium fistulosum small population is designated as an Allium fistulosum population to be intervened;

[0055] S1.3, the unflowering and fruiting plants in the Allium fistulosum population to be intervened are marked with paint;

[0056] S2, the specific steps for determining the optimal growth range for population renewal include the following:

[0057] S2.1, when field investigating the six Allium fistulosum small populations with less plant reserves, the trunk of the flowering and fruiting plant is taken as the center, the area 2 m outward from the crown coverage is divided into annular samples, wherein π=3.14, and the number, height and ground diameter of the seedlings in each annular sample are recorded as population renewal data;

[0058] S2.2, the population renewal data are processed by SPSS 19.0 software to obtain the position range of population renewal, which is used to determine the transplanting position of the Allium fistulosum seedlings, and the statistical results are shown in Table 1;

[0059] S3, domesticating Allium fistulosum seedlings:

[0060] S3.1, the first domestication stage: culturing at normal temperature 28-32℃ to obtain one-year-old Allium fistulosum seedlings, treating the one-year-old Allium fistulosum seedlings at variable temperature amplitude of 4-8℃ for 6 months to obtain one-and-a-half-year-old Allium fistulosum seedlings, and then gradually reducing the temperature at 18℃ with amplitude of 2-3℃, and treating the Allium fistulosum seedlings for 20-30 days each time without frost injury until the low-temperature treatment at 7-10℃ to obtain cold-resistant Allium fistulosum seedlings;

[0061] S3.2, the second domestication stage: setting up a simple cultivation shed in the Allium fistulosum population to be intervened, and moving the cold-resistant Allium fistulosum seedlings to the simple cultivation shed, and opening the top for domestication at regular time, and then removing the simple cultivation shed when the Allium fistulosum seedlings adapt to the climate of the Allium fistulosum population to be intervened;

[0062] S4, obtaining the population update optimal growth range is 9-12m from Table 1, determining the annular band sample of 10m from the tree trunk of the non-flowering and fruiting plant as the transplant pit position, opening the transplant pit at the transplant position, placing the degradable waterproof bag in the transplant pit, setting the nutrient pool at the bottom of the degradable waterproof bag, filling the nutrient pool with small stones, injecting the nutrient solution composed of 7% triacontanol, 39% oligogalacturonide oligosaccharide 1% fumaric acid, 39% potassium dihydrogen phosphate and 14% corn element, vertically placing the garcinia cambogia seedling in the center of the waterproof bag, and part of the root system is immersed in the nutrient solution, and then backfilling the sand and soil in sequence; wherein the degradable waterproof bag is a bag body, and the waterproof coating composed of 73% acrylic acid, 13% sodium dodecyl sulfate and 14% polyacrylamide is sprayed inside and outside the bag.

[0063] The survival rate of the garcinia cambogia seedlings transplanted in the above embodiment 1 is 93%.

[0064] Table 1: The growth position of seedlings and saplings of 6 small garcinia cambogia populations with less plant reserves

[0065]

[0066] The results are shown in Table 1. By investigating the growth position of seedlings and saplings of 6 small garcinia cambogia populations with less plant reserves, the data shows that most of the garcinia cambogia seedlings and saplings are distributed at a distance of 6-9m from the tree trunk. Therefore, when the non-flowering and fruiting plant is artificially intervened, the position at a distance of 4-6 or 9-12m from the tree trunk can be selected as the transplant area to solve the problem of uneven distribution of seedlings and saplings. Further considering the problem of individual competition among populations, the optimal transplant area should be 9-12m to promote photosynthesis of seedlings and saplings, avoid competition for nutrients between garcinia cambogia seedlings and large trees, and reduce the risk of garcinia cambogia falling and injuring seedlings and saplings.

[0067] Embodiment 2

[0068] A method for promoting the regeneration of an endangered plant garcinia cambogia population, the method comprising the following steps:

[0069] S1, conducting a survey to determine the garcinia cambogia population to be intervened, and marking the non-flowering and fruiting plants in the garcinia cambogia population to be intervened;

[0070] Further, step S1 is specifically:

[0071] S1.1, collating the forestry data of garcinia cambogia populations in Guangxi Zhuang Autonomous Region in previous years, sorting the garcinia cambogia populations according to plant reserves, screening out 6 small garcinia cambogia populations with less plant reserves, investigating the position, area and climate of the garcinia cambogia populations, and laying a foundation for field investigation.

[0072] S1.2, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows:

[0073] S1.2.1, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows: 2 2 2 2 2

[0074] S1.2.2, recording the germination of the Allium ampeloprasum population in the seed germination period, using a conspicuous sign to mark the position of the germinated seeds, so as to observe the seedling growth after one year, the number of marked germinated seeds is 53 / 64m 2 ;

[0075] S1.2.3, the seedling period is one year, recording the seedling growth of the marked germinated seeds at the end of the seedling period, the number of seedlings is 21 / 64m 2 ; according to the formula of seedling rate: seedling rate = (number of seedlings / number of germinated seeds) * 100%, i.e. seedling rate = (21 ÷ 53) * 100%, the seedling rate is 39.62%;

[0076] S1.2.4, because the number of seedlings and saplings of the small population of Allium ampeloprasum is too small, and the seedling rate is lower than 60%, the small population of Allium ampeloprasum is designated as the Allium ampeloprasum population to be intervened;

[0077] S1.3, using paint to mark the non-flowering and fruiting plants in the Allium ampeloprasum population to be intervened;

[0078] S2, the specific steps of determining the optimal growth range of population regeneration include the following:

[0079] S2.1, when field investigating 6 small populations of Allium ampeloprasum with less plant reserves, taking the trunk of the flowering and fruiting plant as the center, dividing the area 2m outward from the crown cover according to the annular sample, wherein π = 3.14, recording the number, height and ground diameter of seedlings in each annular sample as the population regeneration data;

[0080] S2.2, the population regeneration data is processed by SPSS 19.0 software to obtain the position range of population regeneration, which is used to determine the transplanting position of Allium ampeloprasum seedlings, and the statistical results are shown in Table 1;

[0081] ​​​​​S3, acclimatize Allium cepa seedlings:

[0082] S3.1, S3.1, the first acclimatization stage: cultivate annual Allium cepa seedlings at normal temperature 28-32℃, and treat annual Allium cepa seedlings at 18-32℃ with a variable temperature range of 4-8℃ for 6 months to obtain one-and-a-half-year-old Allium cepa seedlings, and then gradually reduce the temperature at 18℃ with a range of 2-3℃, and treat Allium cepa seedlings for 20-30 days each time without frost injury until the low temperature treatment at 7-10℃ to obtain cold-resistant Allium cepa seedlings;

[0083] S3.2, the second acclimatization stage: set up a simple cultivation shed in the Allium cepa population to be intervened, and move the cold-resistant Allium cepa seedlings to the simple cultivation shed, and open the top for acclimatization at regular intervals, and then remove the simple cultivation shed after the Allium cepa seedlings adapt to the climate of the Allium cepa population to be intervened;

[0084] S4, obtain the population update optimal growth range from Table 1, determine the annular band-like transplanted pit position at 9m from the trunk of the non-flowering and fruiting plant, dig a transplanted pit at the transplanted position, place a degradable waterproof bag in the transplanted pit, set a nutrient pool at the bottom of the degradable waterproof bag, fill the nutrient pool with small stones, inject a nutrient solution composed of 10% triacontanol, 42% oligogalacturonide oligosaccharide, 0.5% fumaric acid, 42% potassium dihydrogen phosphate and 5.5% corn element, vertically place the Allium cepa seedlings in the center of the waterproof bag, and then backfill sand and original soil after part of the root system is immersed in the nutrient solution; wherein the degradable waterproof bag is a bag body made of a grow bag, and a waterproof coating made of 73% acrylic acid, 13% sodium dodecyl sulfate and 14% polyacrylamide is sprayed inside and outside the grow bag for 3 times.

[0085] The survival rate of the Allium cepa seedlings of the Allium cepa population in the above-mentioned embodiment 2 is 96%.

[0086] Embodiment 3

[0087] A method for promoting the regeneration of an endangered plant Allium cepa population, the method comprising the following steps:

[0088] S1, carry out investigation, and determine the Allium cepa population to be intervened, and mark the non-flowering and fruiting plants in the Allium cepa population to be intervened;

[0089] Further, step S1 is specifically:

[0090] S1.1, sort the Allium cepa population in Guangxi Zhuang Autonomous Region according to the plant reserves, and screen out 6 small Allium cepa populations with less plant reserves, and investigate the location, area and climate of the small Allium cepa populations to lay a foundation for field investigation;

[0091] S1.2, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows:

[0092] S1.2.1, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows: 2 2 2 2 2

[0093] S1.2.2, recording the germination of the Allium ampeloprasum population in the seed germination period, using a conspicuous sign to mark the position of the germinated seeds, so as to observe the seedling growth after one year, the number of marked germinated seeds is 42 per 64 m 2 ;

[0094] S1.2.3, the seedling period is one year, recording the seedling growth of the marked germinated seeds at the end of the seedling period, the number of seedlings is 13 per 64 m 2 , according to the formula of seedling rate: seedling rate = (number of seedlings / number of germinated seeds) * 100%, i.e. seedling rate = (13 ÷ 42) * 100%, the seedling rate is 30.95%;

[0095] S1.2.4, because the number of seedlings and saplings of the small population of Allium ampeloprasum is too small, and the seedling rate is lower than 60%, the small population of Allium ampeloprasum is designated as the Allium ampeloprasum population to be intervened;

[0096] S1.3, using paint to mark the non-flowering and fruiting plants in the Allium ampeloprasum population to be intervened;

[0097] S2, the specific steps of determining the optimal growth range of population regeneration include the following:

[0098] S2.1, when field investigating 6 small populations of Allium ampeloprasum with less plant reserves, taking the trunk of the flowering and fruiting plant as the center, dividing the area 2 m outward from the crown cover according to the annular sample, wherein π = 3.14, recording the number, height and ground diameter of seedlings in each annular sample as the population regeneration data;

[0099] S2.2, the population regeneration data is processed by SPSS 19.0 software to obtain the position range of population regeneration, which is used to determine the transplanting position of Allium ampeloprasum seedlings, and the statistical results are shown in Table 1;

[0100] ​​​​​S3, acclimatize the withania coagulans seedlings:

[0101] S3.1, the first acclimatization stage: cultivate the withania coagulans seedlings at normal temperature 28-32℃ to obtain annual withania coagulans seedlings, and then treat the annual withania coagulans seedlings at 18-32℃ with a variable temperature range of 4-8℃ for 6 months to obtain annual and a half withania coagulans seedlings, and then treat the annual and a half withania coagulans seedlings at 18℃ with a variable temperature range of 2-3℃ to gradually reduce the temperature, and then treat the withania coagulans seedlings for 20-30 days each time until the temperature is reduced to 7-10℃ to obtain cold-resistant withania coagulans seedlings;

[0102] S3.2, the second acclimatization stage: set up a simple cultivation shed in the withania coagulans population to be intervened, and then move the cold-resistant withania coagulans seedlings to the simple cultivation shed, and then open the top of the cultivation shed at regular intervals to acclimate the seedlings, and then remove the simple cultivation shed after the withania coagulans seedlings adapt to the climate of the withania coagulans population to be intervened;

[0103] S4, obtain the population update best growth range from Table 1, determine a circular band sample with a distance of 12 m from the unflowering and fruiting plant trunk as the transplanting pit position, dig a transplanting pit at the transplanting position, place a degradable waterproof bag in the transplanting pit, set a nutrient pool at the bottom of the degradable waterproof bag, fill the nutrient pool with small stones, inject a nutrient solution composed of 7% triacontanol, 39% oligogalacturonide oligosaccharide, 1% fumaric acid, 39% potassium dihydrogen phosphate and 14% corn element, vertically place the withania coagulans seedlings in the center of the waterproof bag, and then fill the sand and the original soil in sequence after part of the root system is immersed in the nutrient solution; wherein the degradable waterproof bag is a bag body made of a grow bag, and a waterproof coating made of 73% acrylic acid, 13% sodium dodecyl sulfate and 14% polyacrylamide is sprayed inside and outside the grow bag for 3 times.

[0104] The transplanting survival rate of the withania coagulans population in the above embodiment 3 is 100%.

[0105] Embodiment 4

[0106] A method for promoting the regeneration of an endangered plant withania coagulans population, the method comprising the following steps:

[0107] S1, carry out investigation, and determine the withania coagulans population to be intervened, and mark the unflowering and fruiting plants in the withania coagulans population to be intervened;

[0108] Further, step S1 is specifically:

[0109] S1.1, sort the withania coagulans population according to the plant reserves by collating the forestry data of the withania coagulans population in Guangxi Zhuang Autonomous Region in previous years, and screen out 6 small withania coagulans populations with less plant reserves, and investigate the location, area and climate of the withania coagulans small populations to lay a foundation for field investigation.

[0110] S1.2. Conduct a field survey on a small population of garlic fruit with a small plant reserve to identify the population of garlic fruit to be intervened. The specific steps of the field survey are as follows:

[0111] S1.2.1: A small population of garlic fruit with a small plant reserve was selected as the survey object. The total number of seedlings and saplings in the field survey population was 44. The small population of garlic fruit covered an area of ​​127m 2 According to the formula, the number of seedlings and saplings = the total number of seedlings and saplings divided by the land area and multiplied by 64m 2 , that is, the number of seedlings and saplings = 44 ÷ 127m 2 ×64m 2 The number of seedlings and saplings of this garlic fruit population was 22 / 64m 2 ;

[0112] S1.2.2. During the seed germination period, record the germination status of the garlic fruit population. Use eye-catching signs to mark the location of the germinated seeds to facilitate observation of their seedling status one year later. The number of germinated seeds marked is 52 per 64m2. 2 ;

[0113] S1.2.3, the seedling period is one year. At the end of the seedling period, record the seedling status of the marked germinated seeds. The number of seedlings is 32 per 64m 2 According to the formula of seedling rate: seedling rate = (number of seedlings / germinated seeds) * 100%, that is, seedling rate = (32÷52) × 100%, the calculated seedling rate is 61.54%;

[0114] S1.2.4, because the number of seedlings and saplings in the garlic fruit small population is large and the seedling rate is higher than 60%, this garlic fruit small population is not set as the garlic fruit population to be intervened.

[0115] Example 5

[0116] A method for promoting the regeneration of the endangered plant garlic fruit population, the method comprising the following steps:

[0117] S1, conducting an investigation to identify the garlic fruit population to be intervened, and marking the non-flowering and fruit-bearing plants in the garlic fruit population to be intervened;

[0118] Furthermore, step S1 is specifically as follows:

[0119] S1.1. Organize forestry data from previous years on garlic fruit populations in the Guangxi Zhuang Autonomous Region, rank them according to plant reserves, identify six small garlic fruit populations with relatively small plant reserves, and investigate their locations, land areas, and climate conditions to lay the foundation for field investigations.

[0120] S1.2, taking a small population of Allium ampeloprasum with less plant reserves as the investigation object, conducting field investigation on one small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows:

[0121] S1.2.1, taking a small population of Allium ampeloprasum with less plant reserves as the investigation object, field investigating that the total number of seedlings and saplings in the population is 50, and the area occupied by the small population of Allium ampeloprasum is 146m 2 According to the formula: number of seedlings and saplings = total number of seedlings and saplings divided by area occupied and multiplied by 64m 2 , i.e. number of seedlings and saplings = 50 ÷ 146m 2 × 64m 2 , the number of seedlings and saplings of the small population of Allium ampeloprasum is obtained as 22 / 64m 2 ;

[0122] S1.2.2, recording the germination of the Allium ampeloprasum population during the seed germination period, marking the position of the germinated seeds by using eye-catching signboards to facilitate observation of the seedling growth after one year, and the number of marked germinated seeds is 46 / 64m 2 ;

[0123] S1.2.3, the seedling growth period is one year, and the seedling growth of the marked germinated seeds is recorded at the end of the seedling growth period, and the number of seedlings is 29 / 64m 2 , according to the formula of seedling rate: seedling rate = (number of seedlings ÷ number of germinated seeds) * 100%, i.e. seedling rate = (29 ÷ 46) * 100%, the seedling rate is calculated as 63.04%;

[0124] S1.2.4, because the number of seedlings and saplings of the small population of Allium ampeloprasum is relatively large, and the seedling rate is lower than 60%, the small population of Allium ampeloprasum is not set as the Allium ampeloprasum population to be intervened.

[0125] Example 6

[0126] A method for promoting the regeneration of an endangered plant Allium ampeloprasum population, the method comprising the following steps:

[0127] S1, conducting investigation to determine the Allium ampeloprasum population to be intervened, and marking non-flowering and fruiting plants in the Allium ampeloprasum population to be intervened;

[0128] Further, step S1 is specifically as follows:

[0129] S1.1, sorting the forestry data of Allium ampeloprasum populations in Guangxi Zhuang Autonomous Region in previous years, sorting the Allium ampeloprasum populations according to plant reserves, screening out 6 small populations of Allium ampeloprasum with less plant reserves, investigating the location, area occupied and climate of the small populations of Allium ampeloprasum, and laying a foundation for field investigation;

[0130] S1.2, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows:

[0131] S1.2.1, taking a small population of Allium ampeloprasum as the investigation object, field investigation is conducted on the small population of Allium ampeloprasum to obtain the Allium ampeloprasum population to be intervened; wherein the specific steps of field investigation are as follows: 2 2 2 2 2

[0132] S1.2.2, recording the germination of the Allium ampeloprasum population in the seed germination period, using a conspicuous sign to mark the position of the germinated seeds, so as to observe the seedling growth after one year, the number of marked germinated seeds is 39 / 64m 2 ;

[0133] S1.2.3, the seedling period is one year, recording the seedling growth of the marked germinated seeds at the end of the seedling period, the number of seedlings is 21 / 64m 2 ; according to the formula of seedling rate: seedling rate = (number of seedlings / number of germinated seeds) * 100%, i.e. seedling rate = (21 ÷ 39) * 100%, the seedling rate is 53.84%;

[0134] S1.2.4, because the number of seedlings and saplings of the small population of Allium ampeloprasum is too small, and the seedling rate is lower than 60%, the small population of Allium ampeloprasum is designated as the Allium ampeloprasum population to be intervened;

[0135] S1.3, using paint to mark the non-flowering and fruiting plants in the Allium ampeloprasum population to be intervened;

[0136] S2, the specific steps of determining the optimal growth range of population regeneration include the following:

[0137] S2.1, when field investigating 6 small populations of Allium ampeloprasum with less plant reserves, taking the flowering and fruiting plant stem of the small population of Allium ampeloprasum as the center, the area 2 m away from the stem to the crown cover is divided into annular samples, wherein π = 3.14, recording the number, height and ground diameter of seedlings in each annular sample as population regeneration data,

[0138] S2.2, the population regeneration data is processed by SPSS 19.0 software to obtain the position range of population regeneration, which is used to determine the transplanting position of Allium ampeloprasum seedlings, and the statistical results are shown in Table 1; ​​​​​

[0139] S3, acclimatizing Allium cepa seedlings:

[0140] S3.1, the first acclimatization stage: culturing the annual Allium cepa seedlings at normal temperature 28-32℃, treating the annual Allium cepa seedlings at 18-32℃ with a variable temperature range of 4-8℃ for 6 months to obtain one-and-a-half-year-old Allium cepa seedlings, and then gradually reducing the temperature at 18℃ with a range of 2-3℃, treating the Allium cepa seedlings for 20-30 days each time until the low-temperature treatment at 7-10℃ to obtain cold-resistant Allium cepa seedlings;

[0141] S3.2, the second acclimatization stage: setting up a simple cultivation shed in the Allium cepa population to be intervened, and then moving the cold-resistant Allium cepa seedlings to the simple cultivation shed, opening the top at regular intervals to acclimate the seedlings, and then removing the simple cultivation shed when the Allium cepa seedlings adapt to the climate of the Allium cepa population to be intervened;

[0142] S4, obtaining the population update from Table 1, determining the annular band-like position of the transplanting pit at 9m from the trunk of the non-flowering and fruiting plant, opening the transplanting pit at the transplanting position, placing a degradable waterproof bag in the transplanting pit, setting a nutrient pool at the bottom of the degradable waterproof bag, filling the nutrient pool with small stones, injecting a nutrient solution composed of 7% triacontanol, 39% oligogalacturonide oligosaccharide, 1% fumaric acid, 39% potassium dihydrogen phosphate, and 14% corn element, vertically placing the Allium cepa seedlings in the center of the waterproof bag, and then backfilling the sand and the original soil after part of the root system is immersed in the nutrient solution; wherein the degradable waterproof bag is made of a Meisheng bag as the bag body, and a waterproof coating composed of 73% acrylic acid, 8% sodium dodecyl sulfate, 9% polyacrylamide, and 10% water is sprayed inside and outside the Meisheng bag for 5 times.

[0143] The transplanting survival rate of the Allium cepa population in the above-mentioned Example 6 is 93%.

[0144] Comparative Example 1

[0145] The same as Example 6, the difference lies in that multiple groups of control experiments are set for the nutrient solution of S4.

[0146] Control Test 1: 10 groups of experiments are set by replacing the nutrient solution with the same volume of ordinary water.

[0147] Control Test 2: 10 groups of experiments are set by using a nutrient solution composed of oligogalacturonide oligosaccharide, potassium dihydrogen phosphate, and corn element.

[0148] Control Test 3: 10 groups of experiments are set by using a nutrient solution composed of triacontanol, fumaric acid, and corn element.

[0149] Control Test 4: 10 groups of experiments are set by using a nutrient solution composed of oligogalacturonide oligosaccharide, fumaric acid, and potassium dihydrogen phosphate.

[0150] The garlic fruit seedlings transplanted in the same garlic fruit population were cultured with the above-mentioned nutrient solution, and the results are shown in Table 2.

[0151] Table 2 Influence of different nutrient solutions on the growth of garlic fruit seedlings

[0152]

[0153] The leaves, leaf crowns and ground diameters of four-year-old garlic fruit seedlings were classified according to the growth grades of garlic fruit. The growth grades are shown in Table 3, and the results are shown in Table 4.

[0154] Table 3 Growth grades of garlic fruit

[0155] Grade Grade Description Tree Shape Crown Width Ground Diameter A Growth Vigour Obvious trunk and many lateral branches, green and lush leaves, large and flat leaves with luster Large crown, crown width > 46 cm Ground diameter > 0.7 cm B Good growth Obvious trunk and many lateral branches, green leaves, large and flat leaves with luster Crown width 36-45 cm Ground diameter 0.6-0.7 cm C Poor growth No obvious trunk or few lateral branches, yellow spots on leaves, small leaves or no luster Crown width 26-35 cm Ground diameter 0.5-0.6 cm D Declining growth Most leaves yellow, and some with yellow or brown spots, insect holes Crown width ≤ 25 cm Ground diameter ≤ 0.5 cm

[0156] Table 4 Influence of nutrient solution components on the growth of garlic fruit

[0157]

[0158] As can be seen from Tables 2-4, oligogalacturonide oligosaccharide maintains cell constant pressure and improves cell membrane permeability, potassium dihydrogen phosphate maintains K element stability, together promotes the absorption of nutrient solution components by root hairs, triacontanol enhances cell oxidase activity, promotes chlorophyll formation and accumulation, zeatin promotes root system increase and elongation, and fumaric acid promotes root system robustness and haustorium formation. By comparing Example 6 with Comparative Example 1, the use of the nutrient solution significantly improves the survival of transplanted garlic fruit seedlings, supplies the garlic fruit seedlings with nutrients through the root system, and oligogalacturonide oligosaccharide and triacontanol enter the seedlings together to improve the stress resistance of the seedlings, improve their own resistance, increase the survival rate in the wild, and increase the number of root systems and leaves of the garlic fruit seedlings, so that the plant grows faster and the tree crown has a larger crown width.

[0159] Comparative Example 2

[0160] The preparation materials and components of the degradable waterproof bag are different.

[0161] Waterproof bag 1: using a grow bag as the bag body, spraying 5 times of waterproof paint consisting of 73% acrylic acid, 8% sodium dodecyl sulfate, 9% polyacrylamide and 10% water inside and outside the grow bag.

[0162] Waterproof bag 2: the preparation method is basically the same as that of waterproof bag 1, and the difference lies in that the waterproof paint consists of 85% acrylic acid and 15% water.

[0163] Waterproof bag 3: the preparation method is basically the same as that of waterproof bag 1, and the difference lies in that the waterproof paint consists of 73% polyurethane solution, 8% sodium dodecyl sulfate, 9% polyacrylamide and 10% water.

[0164] Waterproof bag 4: the preparation method is basically the same as waterproof bag 1, the difference is that the waterproof coating is composed of 50% styrene-acrylic emulsion, 30% stone powder, 10% polyvinyl alcohol and 10% water.

[0165] The adhesion was detected by cutting a corner of the waterproof bag, and the coating was pulled with the same force; the water permeability was detected by using a laboratory suction filtration device; the water resistance was detected by cutting a part of the waterproof bag and soaking it in alkaline water for 7 days, and observing the swelling. The quality results are shown in Table 5.

[0166] Table 5 Water resistance and water permeability of waterproof bags made of different waterproof coatings

[0167] Adhesion Water Resistance Water Permeability Waterproof Bag 1 Cannot be torn off None Impermeable Waterproof Bag 2 Easily torn off Severe bagging Water permeable Waterproof Bag 3 Cannot be torn off Minor bagging Impermeable Waterproof Bag 4 Relatively difficult to tear off None Water permeable

[0168] The degradable waterproof bag obtained by the method has good adhesion and water resistance, can withstand long-term nutrient solution immersion, has good water permeability to prevent nutrient solution loss and forms a relatively closed environment for garlic fruit seedlings, preventing rapid temperature changes. Compared with waterproof bag 2, waterproof bag 1 shows that sodium dodecyl sulfonate and polyacrylamide improve the adhesion of acrylic acid to the beauty bag, and improve the tightness between molecules, improve the adhesion and water resistance of the waterproof film, prevent nutrient solution from penetrating and leaking, thereby enhancing the utilization efficiency of the nutrient solution, coating the beauty bag with waterproof coating, delaying the degradation time of the beauty bag, and changing the horizontal root system to downward growth before degradation, promoting deep penetration of garlic fruit, and deep penetration of root system is beneficial to absorption of deep soil nutrients by garlic fruit, while preventing tree collapse.

[0169] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments with various modifications being suited to the particular use contemplated. The scope of the application is intended to be defined by the claims and their equivalents.

Claims

1. A method of promoting population regeneration of an endangered plant, Allosphenoxytophyllum, characterized by, The method comprises the following steps: S1, investigate and obtain the Allium cepa population to be intervened: S1.1, sort out the previous forestry data of each Allium cepa population, and screen out the Allium cepa population with less plant reserves according to the plant reserves; S1.2, take the Allium cepa population with less plant reserves as the investigation object to carry out field investigation, and obtain the Allium cepa population to be intervened; S1.3, mark the non-flowering and fruiting plants in the Allium cepa population to be intervened; S2, determine the best growth range of population renewal: S2.1, taking the tree trunk of the flowering and fruiting plant as the center, the area 2 m outwardly extended from the crown cover is divided into annular samples, and the number, height and ground diameter of seedlings in each annular sample are recorded as population renewal data; S2.2, the population renewal data is processed by SPSS 19.0 software, and the best growth range of population renewal is 9-12 m, which is used to determine the transplanting position of Allium cepa seedlings; S3, acclimate Allium cepa seedlings; S4, according to the best growth range of population renewal, the annular sample 9-12 m away from the tree trunk of the non-flowering and fruiting plant is determined as the transplanting position, a transplanting pit is opened at the transplanting position, a degradable waterproof bag is placed in the transplanting pit, the bottom of the degradable waterproof bag is provided with a nutrient pool, the nutrient pool is filled with small stones, nutrient solution is injected, the Allium cepa seedlings are vertically placed in the center of the degradable waterproof bag, and part of the root system is immersed in the nutrient solution, and then the sand and soil are backfilled in sequence.

2. The method of facilitating population regeneration of an endangered plant Allium sphaerocephalum according to claim 1, characterized in that, The degradable waterproof bag is composed of acrylic acid, an adhesive and a planting bag, the waterproof paint made of acrylic acid and the adhesive is sprayed on the inside and outside of the planting bag, and is dried, and the spraying is performed n times, wherein n>1.

3. The method of facilitating population regeneration of an endangered plant Allium sphaerocephalum according to claim 2, characterized in that, The adhesive comprises one or more of propylene glycol, sodium dodecyl sulfate and polyacrylamide.

4. The method of facilitating population regeneration of an endangered plant Allium sphaerocephbum according to claim 1, wherein, The nutrient solution comprises triacontanol, oligogalacturonide oligosaccharide and fumaric acid.

5. The method of facilitating population regeneration of an endangered plant Allium sphaerocephbum according to claim 4, wherein, The nutrient solution further comprises potassium dihydrogen phosphate and zeatin.

6. The method of facilitating population regeneration of an endangered plant Allium sphaerocephbum according to claim 1, wherein, In the step S3, the specific steps of acclimating the Allium cepa seedlings are as follows: S3.1, first acclimation stage: sequentially subject the Allium cepa seedlings to normal temperature, variable temperature and low temperature treatment to obtain cold-resistant Allium cepa seedlings; S3.2, second acclimation stage: set up a simple cultivation shed in the Allium cepa population to be intervened, move the cold-resistant Allium cepa seedlings to the simple cultivation shed, and open the top of the cultivation shed at regular time to acclimate the seedlings, until the cold-resistant Allium cepa seedlings adapt to the climate of the Allium cepa population to be intervened, and then remove the simple cultivation shed.

7. The method of facilitating population regeneration of an endangered plant Allium sphaerocephalum according to claim 6, characterized by, In the step S3.1, the variable temperature refers to the temperature fluctuating between the normal temperature and the low temperature, the normal temperature is 28-32℃, and the low temperature is 7-10℃.

8. The method of facilitating population regeneration of an endangered plant Allium sphaerocephalum according to claim 7, characterized by, The variable temperature amplitude is 2-4℃.

9. The method of facilitating population regeneration of an endangered plant Allium sphaerocephbum according to claim 1, wherein, In the step S1.2, the specific steps of taking the Allium cepa population with less plant reserves as the investigation object to carry out field investigation and obtain the Allium cepa population to be intervened are as follows: S1.2.1, take the Allium cepa population with less plant reserves as the investigation object, and investigate the number of seedlings and saplings in the population in the field; S1.2.2, record the germination of the Allium cepa population in the seed germination period, and mark the germinated seeds; S1.2.3, record the seedling formation of the germinated seeds in the seedling formation period, and calculate the seedling formation rate; S1.2.4, taking the seedling number, sapling number and seedling rate as indexes, screening out the to-be-intervened Malania oleifera population from the Malania oleifera population with less plant reserves.

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