An insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests and its application.

Abstract

This invention discloses an insecticidal composition for controlling Lepidoptera, Hemiptera, and Thysanoptera pests and its application. The active ingredients of the insecticidal composition are HNPC-A18237 and abamectin, with a mass ratio of HNPC-A18237 to abamectin of 5–1:1–5. This insecticidal composition can be applied to control Lepidoptera, Hemiptera, and Thysanoptera pests. It exhibits a significant synergistic effect, superior to single active ingredients in both rapid and sustained action. Simultaneously, it reduces the risk of pest resistance to abamectin, broadens the application range of HNPC-A18237, and reduces pesticide dosage and cost, thus benefiting environmental protection. It is highly effective in controlling Lepidoptera, Hemiptera, and Thysanoptera pests in vegetables, rice, fruit trees, and flowering crops.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, specifically to an insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests and its application. Background Technology

[0002] HNPC-A18237 is a novel 1-pyridylpyrazole amide insecticide with excellent insecticidal activity. Its chemical name is 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(ethylaminoformyl)phenyl)-N-methyl-1H-pyrazole-5-carboxamide. The chemical structural formula of HNPC-A18237 is shown in Formula 1.

[0003]

[0004] Chinese patent document CN111662269B discloses the preparation process, structural formula, physical properties, and uses of compound HNPC-A18237. HNPC-A18237 has a broad insecticidal spectrum and can be used to control various pests on crops such as rice, vegetables, citrus, and flowers. Examples include armyworms (Mythimna separata), beet armyworms (Spodoptera exigua (Hübner)), cotton bollworms (Prodenia litura (Fabricius)), diamondback moths (Plutella xylostella), cabbage caterpillars (Pieris rapae Linne), rice leaf rollers (Cnaphalocrocis medinalis (Guenee)), and rice stem borers (Chilodonella fasciata). HNPC-A18237 primarily acts as a stomach poison, exhibiting some contact and systemic activity against lepidopteran pests such as aphids (Aphidoidea), whiteflies (Aleyrodidae), and thrips (Thysanoptera). It is fast-acting, has a long residual effect, and is particularly effective against newly hatched or young larvae of lepidopteran pests. It also inhibits the hatching of lepidopteran eggs and is highly effective against aphids, which have piercing-sucking mouthparts. However, the insecticidal efficacy of HNPC-A18237 in combination with different compounds requires further investigation.

[0005] Emamectin benzoate, or "emamectin benzoate" for short, is a novel, highly effective semi-synthetic antibiotic insecticide and acaricide synthesized from the fermentation product emamectin B1. It possesses the characteristics of a biopesticide, including ultra-high efficiency, low toxicity (nearly non-toxic formulation), low residue, and environmental friendliness. Primarily acting as a stomach poison, it exerts its insecticidal effect mainly by acting on insect GABA receptors. The chemical structural formula of emamectin benzoate is shown in Formula 2.

[0006]

[0007] Abamectin is widely used to control various pests on crops such as vegetables, fruit trees, and cotton. It exhibits extremely high insecticidal activity against lepidopteran larvae, but its activity against other pests is relatively low. Furthermore, the extensive and repeated use of abamectin in recent years has led to varying degrees of resistance in lepidopteran pests, resulting in increasingly higher field dosages and higher costs. Therefore, exploring effective compound formulations is of positive significance for addressing the cost issues associated with abamectin resistance and improving its efficacy.

[0008] Mixing different pesticide varieties is an effective way to delay the development of pesticide resistance in pests, but the effects of such mixtures vary. Researching and developing highly efficient, low-toxicity, and environmentally friendly pesticide compositions is of great significance to the sustainable development of agriculture. Summary of the Invention

[0009] The technical problem to be solved by this invention is to overcome the shortcomings of the prior art, especially the technical problem of how to reduce the risk of pests developing resistance to abamectin and broaden the application field of HNPC-A18237. This invention provides an insecticidal composition and its application that has good control effect, fast and long-lasting effect, low risk of pest resistance, low dosage and low cost for controlling Lepidoptera, Hemiptera and Thysanoptera pests.

[0010] To solve the above-mentioned technical problems, the present invention adopts the following technical solution.

[0011] An insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests, wherein the active ingredients of the insecticidal composition are HNPC-A18237, chemically named 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(ethylaminoformyl)phenyl)-N-methyl-1H-pyrazole-5-carboxamide, and abamectin, wherein the mass ratio of HNPC-A18237 to abamectin is 5 to 1:1 to 5.

[0012] In the above-mentioned insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests, preferably, the sum of the mass of HNPC-A18237 and abamectin accounts for 10% to 70% of the total mass of the insecticidal composition.

[0013] Preferably, the insecticidal composition described above for controlling Lepidoptera, Hemiptera, and Thysanoptera pests is formulated as one of the following: emulsion, microemulsion, suspension concentrate, emulsifiable concentrate, wettable powder, and water-dispersible granules.

[0014] Preferably, the insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests described above further contains pesticide adjuvants, which are one or more of the following: exoskeleton material, dispersant, wetting agent, disintegrant, antifreeze agent, thickener, defoamer, emulsifier, solvent, pH adjuster, filler, and water.

[0015] As a general technical concept, the present invention also provides the application of the above-mentioned insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests in the control of Lepidoptera, Hemiptera and Thysanoptera pests.

[0016] In the above-described applications, preferably, the Lepidoptera, Hemiptera, and Thysanoptera pests are Lepidoptera, Hemiptera, and Thysanoptera pests of vegetables, rice, fruit trees, or flower crops.

[0017] Compared with the prior art, the advantages of the present invention are as follows:

[0018] (1) This invention provides an insecticidal composition in which the active ingredient is HNPC-A18237, chemically named 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(ethylaminoformyl)phenyl)-N-methyl-1H-pyrazole-5-carboxamide, and abamectin. Based on indoor screening and field trials, it was found that the combination of HNPC-A18237 and abamectin showed a significant synergistic effect. It is superior to single active ingredients in terms of both rapid effect and long-lasting effect, which can effectively improve the control effect, reduce the amount of active ingredient used, thereby reducing the pollution of the pesticide to the environment and reducing the cost. It can effectively control Lepidoptera, Hemiptera and Thysanoptera pests on vegetables, rice, fruit trees and flower crops.

[0019] (2) In the insecticidal composition of the present invention, the two active ingredients, HNPC-A18237 and abamectin, have different mechanisms of action. The application of the insecticidal composition obtained by combining HNPC-A18237 and abamectin can significantly delay or overcome the development of insecticide resistance in pests, extend the service life, and is beneficial to the integrated management of pests.

[0020] (3) The insecticidal composition of the present invention can control multiple pests at the same time with one application. The combination of the composition reduces the amount of pesticide used, thereby reducing costs, reducing environmental pollution, ensuring crop safety, high use value, and good application prospects. Detailed Implementation

[0021] The present invention will be further described below with reference to specific preferred embodiments, but this does not limit the scope of protection of the present invention. All materials and instruments used in the following embodiments are commercially available, and unless otherwise specified, all proportions in the following embodiments are by weight percentages.

[0022] An insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests according to the present invention, wherein the active ingredients of the insecticidal composition are HNPC-A18237, chemically named 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(ethylaminoformyl)phenyl)-N-methyl-1H-pyrazole-5-carboxamide, and abamectin, wherein the mass ratio of HNPC-A18237 to abamectin is 5 to 1: 1 to 5.

[0023] Preferably, the sum of the masses of HNPC-A18237 and abamectin accounts for 10% to 70% of the total mass of the insecticidal composition.

[0024] Preferably, the insecticidal composition is formulated as one of the following: emulsion, microemulsion, suspension concentrate, emulsifiable concentrate, wettable powder, and water-dispersible granules.

[0025] Preferably, the insecticidal composition further contains pesticide adjuvants, which are one or more of the following: encapsulating material, dispersant, wetting agent, disintegrant, antifreeze, thickener, defoamer, emulsifier, solvent, pH adjuster, filler, and water.

[0026] The insecticidal composition of the present invention for controlling Lepidoptera, Hemiptera and Thysanoptera pests can be used to control Lepidoptera, Hemiptera and Thysanoptera pests, especially Lepidoptera, Hemiptera and Thysanoptera pests of vegetables, rice, fruit trees and flower crops.

[0027] I. Combined toxicity assay

[0028] Indoor toxicity tests were conducted on the mixture of HNPC-A18237 technical grade and 97% abamectin technical grade. The combined effects were evaluated using the co-toxicity coefficient (CTC) method proposed by Sun & Johnson (1960).

[0029] The formula for calculating the co-toxicity coefficient is as follows:

[0030]

[0031] Theoretical Toxicity Index (TTI) of a mixture = Toxicity index of agent A × Percentage of agent A in the mixture (%) + Toxicity index of agent B × Percentage of agent B in the mixture (%);

[0032]

[0033] Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect.

[0034] Example 1: Combined toxicity assay of HNPC-A18237 and abamectin in lepidopteran armyworms.

[0035] Test target: Third-instar armyworm larvae of uniform size and physiological state, reared indoors for generations.

[0036] Experimental Methods: Refer to the bioassay standard method NY / T1154.9-2008. A spray method was used: Prepare a stock solution of the test agent at a certain concentration, then dilute it with water to six different concentrations for use, with water as a control. Cut fresh corn seedling leaves into 80-90 mm segments and place them in 90 mm diameter petri dishes lined with moisturizing filter paper. Place four leaf segments in each dish. Add at least 10 third-instar armyworm larvae (starved for 4 hours) to each petri dish. Spray the dishes under a spray tower, repeating four times. After treatment, cover the dishes and place them in an observation room for incubation. After 72 hours, investigate and record the mortality rate of the test insects, calculate the mortality rate (%), and use probability analysis to determine the toxicity regression equation, correlation coefficient (r), and median lethal concentration (LC). 50 ).

[0037] Table 1. Toxicity determination of HNPC-A18237 with abamectin and different ratios against armyworms (72h)

[0038]

[0039]

[0040] As shown in Table 1, HNPC-A18237 and abamectin at mass ratios of 5:1, 3:1, 1:1, 1:3, and 1:5 all showed a significant synergistic effect on armyworms, with their LC50... 50 The values ​​were 0.26 mg / L, 0.22 mg / L, 0.15 mg / L, 0.13 mg / L and 0.12 mg / L, respectively, and the co-toxicity coefficients were 126.89, 136.67, 153.11, 144.31 and 149.25, respectively.

[0041] Example 2: Combined toxicity test of HNPC-A18237 and abamectin on diamondback moth (Lepidoptera).

[0042] Test target: Third instar larvae of the diamondback moth of cabbage, which were reared indoors for generations and were of uniform size and physiological condition.

[0043] Experimental Method: Following the bioassay standard method NY / T1154.14-2008, the leaf-dip method was employed. Cabbage leaves were punched into leaf discs using an 18mm diameter punch. The leaf discs were immersed in the test solution for 10 seconds, then placed in 90mm diameter petri dishes lined with moisturizing filter paper. Five leaf discs were placed in each dish. After air drying, at least 10 third-instar larvae of diamondback moths (starved for 4 hours) were added to each petri dish, with four replicates. After treatment, the dishes were covered and placed in an observation room for incubation. After 72 hours, the mortality rate of the test insects was recorded, and the mortality rate (%) was calculated. The toxicity regression equation, correlation coefficient (r), and median lethal concentration (LC) were determined using probability value analysis. 50 ).

[0044] Table 2. Toxicity determination of HNPC-A18237 with abamectin and different ratios against diamondback moth (Lepidoptera) (72h)

[0045]

[0046] As shown in Table 2, HNPC-A18237 and abamectin at mass ratios of 5:1, 3:1, 1:1, 1:3, and 1:5 all showed a significant synergistic effect on diamondback moth, with their LC... 50 The values ​​were 1.17 mg / L, 0.95 mg / L, 0.61 mg / L, 0.50 mg / L and 0.46 mg / L, respectively, and the co-toxicity coefficients were 125.77, 132.82, 146.99, 139.37 and 140.19, respectively.

[0047] Example 3: Combined toxicity test of HNPC-A18237 and abamectin on hemiptera aphids.

[0048] Test target: 3-day-old nymphs of broad bean aphids of the same size and physiological state, reared indoors for generations.

[0049] Experimental Methods: Following the bioassay standard method NY / T1154.6-2006, the immersion method was used: Broad bean seedlings infested with 3-day-old broad bean aphid nymphs were cut off, immersed in the prepared pesticide solution for 10 seconds, then removed. Excess pesticide solution around the plant and insects was absorbed with filter paper. The seedlings were then placed on a water-soaked sponge, covered with a lantern cover, and repeated four times. After treatment, the seedlings were placed in an observation room for cultivation. After 48 hours, the mortality rate of the test insects was investigated and recorded, the mortality rate (%) was calculated, and the toxicity regression equation, correlation coefficient (r), and median lethal concentration (LC50) were determined using probability value analysis. 50 ).

[0050] Table 3. Toxicity determination of HNPC-A18237 with abamectin and different ratios against hemiptera aphids (48h)

[0051]

[0052] As shown in Table 3, HNPC-A18237 mixed with abamectin at mass ratios of 5:1, 3:1, 1:1, 1:3, and 1:5 all showed a significant synergistic effect on aphids, with its LC50... 50 The values ​​were 0.23 mg / L, 0.22 mg / L, 0.21 mg / L, 0.28 mg / L and 0.27 mg / L, respectively, and the co-toxicity coefficients were 136.17, 148.51, 177.27, 154.79 and 168.28, respectively.

[0053] Example 4: Combined toxicity assay of HNPC-A18237 and abamectin in thrips (Thysanoptera).

[0054] Test target: Adult cowpea thrips of uniform size and physiological state, reared indoors for generations.

[0055] Experimental Methods: Following the bioassay standard method NY / T1154.6-2006, the immersion method was used: Cowpea leaves infested with adult thrips were cut, with at least 30 thrips per replicate. The leaves were immersed in the prepared pesticide solution for 10 seconds, then removed. Excess pesticide solution was absorbed from the plant and the area around the thrips using filter paper. The leaves were placed in a 90mm diameter petri dish lined with moisturizing filter paper, and the dish was sealed with plastic wrap. Small holes were pricked in the wrap for ventilation. This was repeated four times. After treatment, the dishes were placed in an observation room for incubation. After 72 hours, the mortality rate of the thrips was recorded, and the mortality rate (%) was calculated. The toxicity regression equation, correlation coefficient (r), and median lethal concentration (LC50) were determined using probability value analysis. 50 ).

[0056] Table 4. Toxicity determination of HNPC-A18237 with abamectin and different ratios against thrips (72h)

[0057]

[0058] As shown in Table 4, HNPC-A18237 mixed with abamectin at ratios of 5:1, 3:1, 1:1, 1:3, and 1:5 all showed a significant synergistic effect on thrips, with its LC50... 50 The values ​​were 3.02 mg / L, 2.36 mg / L, 1.29 mg / L, 1.05 mg / L and 0.93 mg / L, respectively, and the co-toxicity coefficients were 129.67, 134.56, 156.08, 141.25 and 146.20, respectively.

[0059] II. Field efficacy trials

[0060] Test reagent 1: 15% HNPC-A18237·Abamectin (12+3) water emulsion

[0061] A 15% HNPC-A18237·emamectin benzoate emulsion was prepared by mixing 12% HNPC-A18237, 3% abamectin, 10% triphenylethylphenol polyoxyethylene polyoxypropylene block copolymer (emulsifier), 2% ethylene glycol (antifreeze agent), 15% butyl acetate (solvent), 0.5% ethylene glycol stearate (defoamer), and water to 100%.

[0062] Test reagent 2: 12% HNPC-A18237·Abamectin (9+3) suspension

[0063] The following ingredients were mixed: 9% HNPC-A18237, 3% abamectin, 4% sodium naphthalene sulfonate formaldehyde condensate (dispersant), 3% sodium succinate sulfonate (wetting agent), 0.1% polyvinylpyrrolidone (thickener), 1% propylene glycol (antifreeze agent), 0.1% dimethyl silicone oil (defoamer), and water to make up to 100%. The mixture was then sand-milled to obtain a 12% HNPC-A18237·abamectin suspension.

[0064] Control agent 1: 5% HNPC-A18237 emulsifiable concentrate

[0065] Control agent 2: 5% abamectin microemulsion

[0066] Example 5: Field efficacy test of HNPC-A18237, abamectin and their combination for controlling diamondback moth in cabbage.

[0067] Experimental Methods: Field efficacy trials were conducted strictly in accordance with the People's Republic of China National Standard (GB / T 17980.13-2000). The experimental field had moderately tilled soil, was clayey soil with good fertility, and had convenient irrigation and drainage. Each treatment was replicated four times in a randomized block design. The initial insect population was assessed before application, and again at 1, 3, and 7 days after application, for a total of four surveys. Five points were randomly marked in each plot, with two plants surveyed at each point, for a total of ten plants. The number of pests on the leaves of each cabbage plant was investigated, the number of live insects was counted, and the insect population reduction rate and control effect were calculated.

[0068] Insect population reduction rate (%) = (Insect population before treatment - Number of surviving insects after treatment) / Insect population before treatment × 100;

[0069] Control efficacy (%) = [1 - (number of insects in the control area before treatment × number of insects in the treatment area after treatment) / (number of insects in the control area after treatment × number of insects in the treatment area before treatment)] × 100.

[0070] Table 5. Field efficacy test results of HNPC-A18237, abamectin and its combinations in controlling diamondback moth in cabbage.

[0071]

[0072] Note: The Duncan multiple range test was used. The same letters in the table indicate that the difference between treatments is not significant (lowercase p = 0.05, uppercase p = 0.01).

[0073] As shown in Table 5, one day after application, the pesticide composition of the present invention achieved a control efficacy of over 60.02% against the diamondback moth in cabbage, significantly superior to single-agent formulations. Three and seven days after application, the pesticide composition of the present invention also achieved a control efficacy of over 74.83% against the diamondback moth in cabbage, again significantly superior to single-agent formulations. This indicates that the insecticidal composition of the present invention is superior to single-agent formulations in both rapid and sustained action, and is safe for cabbage without causing phytotoxicity.

[0074] Example 6: Field efficacy test of HNPC-A18237, abamectin and their combination for controlling cowpea thrips.

[0075] Experimental Methods: Field efficacy trials were conducted strictly in accordance with the People's Republic of China industry standard (NY / T 1464.6-2007). The experimental field had moderately tilled soil, was clayey soil with good fertility, and had convenient irrigation and drainage. Each treatment was replicated four times in a randomized block design. The insect population was assessed before application and again at 1, 3, 7, and 10 days after application, for a total of five assessments. Five sampling points were taken from each plot, with two plants sampled at each point. Three bean pods were selected from each plant to determine the number of surviving nymphs. The insect population reduction rate and control effect were calculated. Insect population reduction rate (%) = (Insect population before treatment - Number of surviving insects after treatment) / Insect population before treatment × 100;

[0076] Control efficacy (%) = (1 - (number of insects in the control area before treatment × number of insects in the treatment area after treatment) / (number of insects in the control area after treatment × number of insects in the treatment area before treatment)) × 100.

[0077] Table 6. Field efficacy test results of HNPC-A18237, abamectin and its combinations in controlling cowpea thrips.

[0078]

[0079] Note: The Duncan multiple range test was used. The same letters in the table indicate that the difference between treatments is not significant (lowercase p = 0.05, uppercase p = 0.01).

[0080] As shown in Table 6, one day after application, the pesticide composition of this invention maintained a control efficacy of over 81.12% against cowpea thrips, significantly superior to single-agent formulations. Three, seven, and ten days after application, the pesticide composition maintained a control efficacy of over 83.72% against cowpea thrips, also significantly superior to single-agent formulations. This indicates that the insecticidal composition of this invention is superior to single-agent formulations in both rapid and sustained action, and is safe for cowpeas without phytotoxicity.

[0081] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make many possible variations and modifications to the technical solutions of the present invention using the methods and techniques disclosed above, or modify them into equivalent embodiments with equivalent changes, without departing from the spirit and technical essence of the present invention. Therefore, any simple modifications, equivalent substitutions, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solutions of the present invention shall still fall within the protection scope of the technical solutions of the present invention.

Claims

1. An insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests, characterized by, The active ingredients of the insecticidal composition are HNPC-A18237, chemically named 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(ethylaminoformyl)phenyl)-N-methyl-1H-pyrazole-5-carboxamide, and abamectin, wherein the mass ratio of HNPC-A18237 to abamectin is 5 to 1: 1 to 5.

2. The insecticidal composition for controlling lepidopteran, hemipteran and blattodean pests according to claim 1, characterized by, The sum of the mass of HNPC-A18237 and abamectin accounts for 10% to 70% of the total mass of the insecticidal composition.

3. The insecticidal composition for controlling lepidopteran, hemipteran and blattodean pests according to claim 1 or 2, characterized in that, The insecticidal composition is formulated as one of the following: emulsion, microemulsion, suspension concentrate, emulsifiable concentrate, wettable powder, and water-dispersible granules.

4. The insecticidal composition for controlling Lepidoptera, Hemiptera, and Thysanoptera pests according to claim 1 or 2, characterized in that, The insecticidal composition also contains pesticide adjuvants, which are one or more of the following: encapsulating material, dispersant, wetting agent, disintegrant, antifreeze, thickener, defoamer, emulsifier, solvent, pH adjuster, filler, and water.

5. The use of an insecticidal composition for controlling Lepidoptera, Hemiptera and Thysanoptera pests as described in any one of claims 1 to 4 in controlling Lepidoptera, Hemiptera and Thysanoptera pests.

6. The application according to claim 5, characterized in that, The Lepidoptera, Hemiptera, and Thysanoptera pests mentioned refer to Lepidoptera, Hemiptera, and Thysanoptera pests of vegetables, rice, fruit trees, or flower crops.

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