disinfectant

Abstract

The present application provides a new disinfectant, comprising: active agent, reducing agent and buffer. In a particularly preferred embodiment, the disinfectant consists of: active agent, reducing agent, protective agent, chelating agent, buffer and adjuvant. The disinfectant of the present application has a broad-spectrum disinfection effect, and can be widely used for disinfection of various microorganisms such as bacteria, fungi and viruses, especially for disinfection of new coronavirus.

Description

[0001] Cross-reference information

[0002] This application claims priority to the Chinese patent application No. 202210957717.X, filed on August 10, 2022, to the Chinese Patent Office, the content of which is incorporated herein by reference in its entirety. TECHNICAL FIELD

[0003] The present application relates to the field of disinfectants, especially disinfectants suitable for killing new coronaviruses. BACKGROUND

[0004] The disinfectants on the market at present mainly include the following categories. 1. Chlorine-containing disinfectants, such as 84 disinfectant, which has an effective chlorine content of 5.5-6.5%, can kill various microorganisms, and is suitable for disinfecting object surfaces, environments, etc.; however, this disinfectant requires "high concentration" and "long time" to achieve good bactericidal effect, and is unstable, easily decomposed into toxic and corrosive fumes at high temperatures, causing damage to the human body. 2. Peroxide disinfectants, such as 3% hydrogen peroxide disinfectant, which has strong oxidizing properties and can effectively kill enteric pathogenic bacteria, pyogenic cocci, and pathogenic yeasts, and is generally used for object surface disinfection; however, this disinfectant is corrosive to metals and is not suitable for object surface disinfection in ports, logistics, etc. 3. Alcohol disinfectants, such as 75% ethanol, which is suitable for object surface and human skin disinfection; however, it requires soaking or repeated wiping to achieve good results for object disinfection, and due to its flammability and volatility, it is not suitable for disinfection in crowded places. It is currently mainly used for medical disinfection.

[0005] Quaternary ammonium salt disinfectants are a broad-spectrum disinfectant. This type of disinfectant has strong bactericidal ability, low effective use concentration, and can effectively kill bacteria, fungi and viruses at a concentration of 0.01-0.1%, and is stable, non-irritating and non-corrosive, and can be directly used for skin and object surface disinfection, and is suitable for large-scale use.

[0006] Benzalkonium chloride, a mixture of chlorinated (N-alkyl-phenyl-dimethyl) ammonium, belongs to the commonly used quaternary ammonium salt surfactants. Because it has a long-chain alkyl group, it can destroy the cell (wall) membrane of microorganisms, causing them to die, thereby achieving the effect of sterilization. Therefore, manufacturers have developed various disinfectants containing benzalkonium chloride. Patent CN102065696B discloses a universal bactericidal disinfectant, which uses benzalkonium chloride, sorbic acid, benzoic acid, etc. as active agents, and has high bactericidal capacity. However, its active pH is 2.5-4.0, which is weakly acidic, and is not suitable for popularization and use. Patent CN201611024695 discloses a composite disinfectant composed of benzalkonium chloride and other surfactants. This disinfectant can efficiently kill 100% (log value > 6) of Escherichia coli, Staphylococcus aureus, Streptococcus albus, and Pseudomonas aeruginosa within 5 minutes. Patent CN201010289039 discloses a composite antibacterial agent and its preparation method. This method uses benzalkonium chloride, didecyldimethylammonium chloride, and ε-polylysine as the main bacteriostatic components, and can kill 100% (log value > 4) of Escherichia coli, Staphylococcus aureus, and Streptococcus albus within 2 minutes. Patent CN202010085304 discloses a formula of skin disinfecting cleaning liquid and its preparation method. This cleaning liquid uses benzalkonium chloride and cetylpyridinium chloride in combination, can kill 100% (log value > 5) of Escherichia coli within 1 minute, and kill 99.9% of skin surface bacteria within 10 minutes. This patent mentions that this method can kill the novel coronavirus, but no specific data is disclosed. SUMMARY

[0007] The present application provides a new type of disinfectant, comprising: an active agent, a reducing agent, and a buffer.

[0008] In a particularly preferred embodiment, the disinfectant consists of the following ingredients: an active agent, a reducing agent, a protective agent, a chelating agent, a buffer, and an auxiliary material.

[0009] The disinfectant of the present application is particularly suitable for killing or inhibiting the novel coronavirus. The disinfectant has the characteristics of short action time, high bactericidal efficacy, and no pollution, and can be applied to human body surface, such as hands, or to public places (such as hospitals, subways, schools, airports), logistics cold chain, or physical surface, such as large containers in ports. DETAILED DESCRIPTION

[0010] Unless otherwise specified, all technical and scientific terms used herein have the meanings commonly understood by one of ordinary skill in the art.

[0011] "Coronavirus" refers to a class of enveloped viruses with single-stranded positive-sense RNA genomes that can cause illness in vertebrates, especially mammals or birds. They are in the taxonomic order Nidovirales, family Coronaviridae. Seven coronaviruses have been discovered to infect humans so far, including HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, MERS-CoV, and SARS-CoV-2. Among the diseases they cause, some symptoms are relatively mild, similar to common flu, while others can be fatal, such as SARS, MERS and COVID-19. The coronavirus that causes COVID-19 is SARS-CoV-2, also known as "new coronavirus".

[0012] Without being bound by any particular theory, the inventors have surprisingly found that the active agent, reducing agent and chelating agent in the disinfectant can play different mechanisms, and their combination can greatly improve the disinfection effect.

[0013] Based on the above findings, the inventors have developed a new broad-spectrum disinfectant, which combines the use of active agents, reducing agents and / or chelating agents, and takes advantage of their different mechanisms, thereby greatly improving the disinfection effect of the disinfectant.

[0014] The "active agent" can be various commonly used active agents suitable for the disinfection of microorganisms such as bacteria and viruses. The preferred active agent is a quaternary ammonium salt surfactant. For example, the preferred active agent can be one or more of the following: benzalkonium chloride, benzalkonium bromide, benzethonium chloride, cetylpyridinium chloride, decamethonium chloride. The particularly preferred active agent is benzalkonium chloride.

[0015] The active agent of the present application can also include one or more other types of active agents, such as anionic surfactants or nonionic surfactants, etc.

[0016] Quaternary ammonium salt disinfectants have the advantages of low bactericidal concentration, no irritation, no toxicity, no corrosion, in addition to the surface adsorption, surface tension reduction and certain washing function of surfactants.

[0017] The active agent in the disinfectant of the present application can be one or more.

[0018] A "buffer" is used to provide a relatively stable solution environment, with the function of buffering pH changes, avoiding the influence of extreme environment on the disinfecting effect of the disinfectant. Preferably, the pH is close to neutral, for example, 5-10. Various commonly used buffers can be used, such as Tris, disodium hydrogen phosphate-sodium dihydrogen phosphate (PB), HEPES, MOPS, etc. A particularly preferred buffer is disodium hydrogen phosphate-sodium dihydrogen phosphate (PB).

[0019] A "chelating agent" can chelate with heavy metal ions in water, reducing the influence of heavy metal ions on the effect of the disinfectant; in addition, the chelating agent has a coordination bond, which can form a stable ligand with metal ions, inactivating metalloproteases on the cell (virus) membrane. Preferred chelating agents include one or more of the following: ethylenediaminetetraacetic acid (EDTA) and its metal salts, nitrilotriacetic acid (NTA) and its metal salts, ethylene glycol diethyl ether diamine tetraacetic acid (EGTA) and its metal salts, diethylene triamine pentaacetic acid (DTPA) and its metal salts. A particularly preferred chelating agent of the present application is EDTA-Na2, etc. The chelating agent in the disinfectant of the present application can be one or more.

[0020] "Metal salts" herein refer to soluble salts formed by an acid (such as EDTA) and a metal ion, such as sodium salt, potassium salt, etc. For example, the "metal salts" of EDTA, such as sodium salt, can also be used as a chelating agent.

[0021] A "reducing agent" refers to a class of compounds that can break the disulfide bond of a protein or polypeptide. Preferred reducing agents include one or more of the following: dithiothreitol (DTT), reduced glutathione, β-mercaptoethanol, mercaptoacetic acid and its metal salts, L-cysteine or tris(2-carboxyethyl)phosphine (TCEP). A particularly preferred reducing agent is L-cysteine. The reducing agent in the disinfectant of the present application can be one or more.

[0022] A "protective agent" refers to an antioxidant that can prevent or alleviate the oxidation of the reducing agent in the disinfectant. Preferred protective agents include one or more of the following: sodium sulfite (Na2SO3), vitamin C, vitamin E. A particularly preferred protective agent is sodium sulfite.

[0023] Preferably, the protective agent is added to the disinfectant to help prevent or alleviate the oxidation of the reducing agent, which has a positive effect on improving the disinfecting effect of the disinfectant.

[0024] As understood by those skilled in the art, if the reducing agent is relatively stable in nature or the amount of reducing agent is large, the protective agent can not be added.

[0025] Preferably, adding a chelating agent to the disinfectant can form a stable ligand with metal ions, thereby inactivating metalloproteinases on the cell membrane (virus) and improving the disinfection effect of the disinfectant.

[0026] This invention provides a novel disinfectant comprising: an active agent, a reducing agent, and a buffer. In some embodiments, the disinfectant consists of an active agent, a reducing agent, a buffer, and optional excipients.

[0027] In some embodiments, the disinfectant comprises an active agent, a reducing agent, a buffer, and a protective agent. In some embodiments, the disinfectant consists of an active agent, a reducing agent, a buffer, a protective agent, and optional excipients.

[0028] In some embodiments, the disinfectant also includes surfactants, reducing agents, buffers, and chelating agents. In some embodiments, the disinfectant consists of surfactants, reducing agents, buffers, chelating agents, and optional excipients.

[0029] In some implementations, the disinfectant includes surfactants, reducing agents, buffers, protectants, and chelating agents.

[0030] In some implementations, the disinfectant consists of the following components: surfactant, reducing agent, protectant, chelating agent, and buffer.

[0031] In some preferred embodiments, the disinfectant consists of the following components: activator, reducing agent, protectant, chelating agent, buffer, and excipients.

[0032] The term "excipients" refers to conventional additives known in the field of disinfectants, other than functional ingredients such as surfactants and reducing agents, that do not significantly affect the disinfection effect of disinfectants. These include, but are not limited to, solvents, fragrances, colorants, preservatives, and wetting agents.

[0033] Those skilled in the art will understand that different types of excipients can be selected to prepare disinfectants suitable for specific use scenarios. For example, for aqueous disinfectants, excipients such as sterile water can be added as solvents; for disinfectants applied directly to the human body surface, such as hand disinfectants, excipients such as wetting agents (e.g., glycerin or hyaluronic acid) or fragrances (e.g., peppermint oil) can be added.

[0034] The term "optional" means that the described object is not essential and may or may not be included. For example, "optional excipients" means that a disinfectant may or may not contain excipients, which can be determined by those skilled in the art based on the specific application scenario.

[0035] In some preferred embodiments, the disinfectant comprises: benzalkonium chloride as an active agent, EDTA-Na2 as a chelating agent, L-cysteine ​​as a reducing agent, sodium sulfite as a protective agent, and disodium hydrogen phosphate-sodium dihydrogen phosphate (PB) as a buffer.

[0036] In some particularly preferred embodiments, the disinfectant comprises: benzalkonium chloride as an active agent, EDTA-Na2 as a chelating agent, L-cysteine ​​as a reducing agent, sodium sulfite as a protective agent, and disodium hydrogen phosphate-sodium dihydrogen phosphate (PB) as a buffer.

[0037] In some particularly preferred embodiments, the disinfectant comprises: benzalkonium chloride as an active agent, EDTA-Na2 as a chelating agent, L-cysteine ​​as a reducing agent, sodium sulfite as a protective agent, disodium hydrogen phosphate (PB) as a buffer, and excipients.

[0038] In some implementations, the concentration of the quaternary ammonium salt surfactant used as the active agent is 0.001%-1.0%.

[0039] The concentration (w / v) can be, for example, 0.001%, 0.002%, 0.003%, 0.004%, 0.005%, 0.006%, 0.007%, 0.008%, or 0.009% (w / v). In some embodiments, the concentration of the quaternary ammonium salt surfactant as the active agent is 0.01%-0.1% (w / v). In some preferred embodiments, the concentration of benzalkonium chloride as the active agent is 0.01%-0.1% (w / v).

[0040] In some embodiments, the concentration of the chelating agent is in the range of 0.01-10.0 mM, for example, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, or 0.1 mM. In some preferred embodiments, the concentration of EDTA-Na2 as the chelating agent is 0.05-10.0 mM.

[0041] In some embodiments, the concentration of the reducing agent is in the range of 0.1-15.0 mM, for example, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5 mM. In some preferred embodiments, the concentration of L-cysteine ​​as the reducing agent is 1.0-15.0 mM.

[0042] In some embodiments, the concentration of the protective agent is in the range of 0.01-50.0 mM, for example, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0 mM. In some preferred embodiments, the concentration of sodium sulfite (Na₂SO₃) as the protective agent is 1.0-50.0 mM.

[0043] Those skilled in the art will understand that, due to the complementary nature of the mechanisms of action of the surfactant, reducing agent, and / or chelating agent, the concentration of the surfactant can be further reduced when the concentration of the reducing agent and / or chelating agent is further increased. The same applies to the reducing agent and chelating agent.

[0044] The disinfectant of this invention has a broad-spectrum disinfection effect and can be widely used for the disinfection of various microorganisms such as bacteria, fungi, and viruses.

[0045] The disinfectant of this invention is particularly suitable for the disinfection of the novel coronavirus. The novel coronavirus is mainly composed of RNA encapsulated by an envelope and nucleocapsid protein. Its spike protein specifically recognizes the ACE2 receptor on the human cell membrane, thereby mediating viral invasion. Currently available disinfectants primarily alter the permeability of the viral envelope, coagulating and denaturing membrane proteins to kill the virus, with a disinfection time exceeding one minute. However, protein denaturation is reversible to some extent; denatured viral proteins can renature under specific conditions, restoring the virus's infectivity. The disinfectant provided by this invention can permanently inactivate the virus within seconds. The principle of this invention's disinfectant in killing the virus is as follows: an active agent disrupts the viral envelope or denatures the nucleocapsid protein; then, under the action of a reducing agent, the protein disulfide bonds are broken, causing an "irreversible" change in the protein structure, thereby completely disinfecting the virus and achieving permanent inactivation.

[0046] Unless otherwise stated, the methods and techniques used in this specification are generally performed in accordance with methods well known and conventional in the art and in the manner described in the various references set forth or cited in this specification.

[0047] Example 1: Preparation of disinfectant

[0048] 1) Disinfectant components:

[0049] Buffer: Sodium dihydrogen phosphate (PB);

[0050] Surfactant: Benzalkonium chloride;

[0051] Chelating agent: EDTA-Na2;

[0052] Reducing agent: L-Cysteine;

[0053] Protectant: Na2SO3.

[0054] 2) Reagent preparation

[0055] Control reagent: 1.0 mM PB, pH 8.0.

[0056] Neutralizing agent: This refers to a reagent that can promptly stop the killing effect of disinfectant (on microorganisms), and the neutralizing agent itself has no inhibitory or killing effect on microorganisms, nor does it have any adverse effect on the culture medium. Dissolve 3.0g of Tween 80 in 100mL of water, weigh 0.3g of lecithin and add it to the solution. After fully dissolving, sterilize at 121℃ for 30 minutes.

[0057] Example 2, Sterilization Experiment

[0058] 1) Preparation of bacterial culture: Staphylococcus aureus (ATCC 6538) and Escherichia coli (CICC 10899) were inoculated onto LB agar plates and incubated at 37°C. After single colonies grew, they were transferred to liquid LB medium and incubated at 37°C. When the OD of the bacterial culture... 600 When the bacterial count reaches 0.6–0.8 (pair count > 8), centrifuge to collect the bacterial cells, discard the supernatant, resuspend the bacterial cells with an equal volume of buffer solution, and place on ice for later use.

[0059] 2) Sterilization experiment: Take 10 μL of bacterial solution into a 1.5 mL EP tube, then add 90 μL of disinfectant to the EP tube, mix well, react at room temperature for 5 seconds, and immediately add 900 μL of neutralizing agent to stop the sterilization reaction. Then spread all the reaction product in the EP tube onto LB agar plates and incubate at 37°C for 16–24 hours.

[0060] 3) Control experiment: Transfer 10 μL of bacterial culture to a 1.5 mL EP tube, then add 90 μL of PB buffer to the EP tube, mix well, react at room temperature for 5 seconds, and immediately add 900 μL of neutralizing agent. Then, serially dilute the reaction product in the EP tube to 10 mL. -8 After multiplying, spread the mixture onto LB solid plates and incubate at 37°C for 16–24 hours.

[0061] In addition, transfer 10 μL of bacterial culture to a 1.5 mL EP tube, and then add 990 μL of neutralizing agent to the EP tube. Incubate at room temperature for 1 minute, and then serially dilute the reaction product in the EP tube to 10 mL. -8After multiplying, spread the mixture onto LB solid plates and incubate at 37°C for 16–24 hours.

[0062] The number of single colonies grown on the plate was used as the quantitative basis. Each disinfection experiment used three parallel plates for testing, and the average number of single colonies on the three plates was taken.

[0063] Example 3: Evaluation of the sterilization effect of disinfectant

[0064] Prepare disinfectant at the following concentrations:

[0065] Table 1

[0066]

[0067] Adjust the pH to 8.0.

[0068] According to the method described in Example 2, the bactericidal potency of the disinfectant and the following components was evaluated:

[0069] I. Buffer: 1.0 mM PB

[0070] II. Activator: 0.01% benzalkonium chloride

[0071] III. A mixture of chelating agent, reducing agent and protective agent: 0.05mM EDTA-Na2+1.5mM L-Cysteine+2.0mM Na2SO3.

[0072] Using the above three groups of reagents and disinfectants, sterilization experiments were conducted according to the method described in Example 2. The results are as follows:

[0073]

[0074] Benzalkonium chloride, when used alone, exhibits strong bactericidal activity, but it cannot achieve 100% bactericidal effect in a short time. However, when benzalkonium chloride is used in combination with the other components shown in Table 1 (i.e., formulated as a disinfectant), a 100% rapid bactericidal effect can be achieved. The results indicate that the other components can improve the disinfection efficiency of the active agent, while the buffer has no disinfection effect.

[0075] Example 4: Exploration of the optimal pH for disinfectants

[0076] Disinfectants were prepared according to the formulation shown in Table 1 of Example 3, and the pH values ​​were adjusted to 7.0, 7.4, 8.0, 8.5, 9.0, and 9.5 using HCl or NaOH, respectively. The kill rate of disinfectants at different pH values ​​against Escherichia coli was tested, and the results are shown in the table below:

[0077]

[0078] The results showed that the bactericidal activity of the disinfectant was not affected when the pH value was in the range of 7.0 to 9.5.

[0079] Example 5: Exploration of the Concentration of Active Ingredients in Disinfectants

[0080] Disinfectant was prepared according to the formula shown in Table 1 of Example 3. With other component concentrations remaining the same as in Table 1, the benzalkonium chloride concentration was adjusted to 0.005%, 0.0075%, 0.01%, 0.05%, 0.075%, and 0.1%, respectively. The kill rate of disinfectants with different benzalkonium chloride concentrations against Escherichia coli was tested, and the results are shown in the table below:

[0081]

[0082] The results showed that in this experiment, the active ingredient (benzalkonium chloride) of the disinfectant could kill 100% of the tested bacteria at concentrations of 0.1%, 0.075%, 0.05%, and 0.01%, but could not kill 100% of the tested bacteria at concentrations of 0.075% and 0.005%. This indicates that the minimum effective concentration of benzalkonium chloride in this experiment is 0.01%.

[0083] Example 6: Exploration of Disinfectant Chelating Agent Concentration

[0084] Disinfectants were prepared according to the formulation shown in Table 1 of Example 3. With other component concentrations remaining the same as in Table 1, disinfectants with EDTA-Na2 concentrations of 0, 0.05, 0.1, 0.5, 5.0, and 10.0 mM were prepared, and their kill rate against Escherichia coli was tested. The results are shown in the table below:

[0085]

[0086] The results showed that the lowest effective concentration of the chelating agent component (EDTA-Na2) of the disinfectant in this experiment was 0.05 mM.

[0087] Example 7: Exploration of Disinfectant Reducing Agent Concentration

[0088] Disinfectants were prepared according to the formulation shown in Table 1 of Example 3, with other component concentrations remaining the same as in Table 1. Disinfectants with L-cysteine ​​concentrations of 0, 0.5, 1.0, 1.5, 3.0, and 15.0 mM were prepared, and their kill rate against Escherichia coli was tested. The results are shown in the table below:

[0089]

[0090] The results showed that the lowest effective concentration of the reducing agent component (L-Cysteine) of the disinfectant was 1.0 mM in this experiment.

[0091] Example 8: Exploration of disinfectant protectant concentration

[0092] Disinfectants were prepared according to the formulation shown in Table 1 of Example 3. With other component concentrations remaining the same as in Table 1, disinfectants with Na2SO3 concentrations of 0, 1.0, 2.0, 20.0, and 50.0 mM were prepared, and their kill rate against Escherichia coli was tested. The results are shown in the table below:

[0093]

[0094] The results showed that the minimum effective concentration of the protective agent component (Na2SO3) of the disinfectant was 1.0 mM in this experiment.

[0095] Example 9: Evaluation of the sterilization effect of disinfectant on Staphylococcus aureus (Gram-positive bacteria)

[0096] Prepare disinfectant at the following concentrations:

[0097] Table 2

[0098]

[0099] Adjust the pH to 8.0.

[0100] The sterilization effect of Staphylococcus aureus was evaluated according to the method described in Example 2, and the results are as follows:

[0101]

[0102] The results showed that the disinfectant could kill approximately 4.8 × 10⁴ cells within 5 seconds with 100% effectiveness. 9 One Staphylococcus aureus. This indicates that the disinfectant is effective not only in killing Gram-negative bacteria (Escherichia coli) but also in killing Gram-positive bacteria.

[0103] Example 10: Evaluation of the bactericidal effect of disinfectant on yeast (fungi).

[0104] Prepare the disinfectant according to Table 2 of Example 9.

[0105] Activated Pichia pastoris GS115 was inoculated into YPD (1% Yeast Extract, 2% Peptone, 2% Glucose) liquid medium and incubated at 28°C for 16–24 hours. 1 mL of the bacterial suspension was taken, centrifuged, and the supernatant was discarded. Then, 1 mL of control reagent (1 mM PB, pH 8.0) was added to resuspend the suspension, and the mixture was placed on ice for later use.

[0106] Add 450 μL of disinfectant to an EP tube, then add 50 μL of the test bacterial suspension and start timing immediately. At 5, 10, 30, and 60 seconds, add 100 μL of the disinfectant product to 900 μL of neutralizing agent. Then, spread the entire 1000 μL of neutralizing product onto a YPD plate. Incubate at 28°C for 2–3 days, count the number of single colonies, and calculate the kill rate. The results are as follows:

[0107]

[0108] The results showed that the disinfectant could kill approximately 8.2 × 10⁻⁶ cells within 30 seconds, achieving 100% kill rate. 9 One yeast cell. This indicates that the disinfectant is effective in killing fungal microorganisms.

[0109] Example 11: Evaluation of the inactivation effect of disinfectant on novel coronavirus (SARS-CoV-2) (This experiment needs to be conducted in a P3 laboratory)

[0110] Prepare the disinfectant according to Table 2 of Example 9.

[0111] Prepare the SARS-CoV-2 virus stock solution to be tested (log value > 4).

[0112] 450 μL of disinfectant was placed in an EP tube at room temperature, followed by 50 μL of viral stock solution, and the timer was started immediately. At 5, 10, 30, and 60 seconds, 100 μL of the disinfectant product was added to 900 μL of neutralizing agent. Then, 100 μL of the neutralizing product was used to infect Vero-E6 cells. After incubation at 37°C for 2–3 days, the cells were fixed and stained. The number of white and empty spots was counted, and the inactivation rate of the disinfectant against the novel coronavirus was calculated. The results are as follows:

[0113]

[0114] The results showed that in this experiment, the disinfectant could kill approximately 1.5 × 10⁻⁶ cells within 5 seconds, achieving 100% kill rate. 4 One COVID-19 virus.

Claims

1. Disinfectant, comprising: benzalkonium chloride as an active agent at a concentration of 0.01%-1.0% W / V; EDTA-Na2 as a chelating agent at a concentration of 0.05-10 mM; L-cysteine ​​as a reducing agent at a concentration of 1.0-15.0 mM; sodium sulfite as a protective agent at a concentration of 1.0-50.0 mM; and disodium hydrogen phosphate-sodium dihydrogen phosphate PB as a buffer.

2. The disinfectant of claim 1, wherein the disinfectant comprises the following components: Benzalkonium chloride as an active agent, with a concentration of 0.01%-1.0% W / V; EDTA-Na2 as a chelating agent, with a concentration in the range of 0.05-10 mM; L-cysteine ​​as a reducing agent, with a concentration in the range of 1.0-15.0 mM; sodium sulfite as a protective agent, with a concentration in the range of 1.0-50.0 mM; and disodium hydrogen phosphate-sodium dihydrogen phosphate (PB) as a buffer.

3. The disinfectant according to claim 1, wherein the disinfectant comprises the following components: Benzalkonium chloride as an active agent, EDTA-Na2 as a chelating agent, L-cysteine ​​as a reducing agent, sodium sulfite as a protective agent, disodium hydrogen phosphate-sodium dihydrogen phosphate (PB) as a buffer, and excipients.

4. The use of the disinfectant as described in any one of claims 1-3 in the preparation of an agent for disinfecting or inhibiting the novel coronavirus.

5. The application as described in claim 4, wherein the disinfectant is applied to a human body surface.

6. The application as described in claim 4, wherein the disinfectant is applied to the surface of an object.

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