Formulations affecting leafhopper tropistic behavior and their use in leafhopper attraction or repulsion

Abstract

The application discloses a preparation for influencing the tropotaxis behavior of leafhoppers and application of the preparation in leafhopper attraction or avoidance, and belongs to the technical field of pest control. The preparation for influencing the tropotaxis behavior of leafhoppers has effective components containing at least one of phenylacetone, pinene, linalool, ocimene, linalool oxide, alpha-pinene, sabinene, eucalyptol, heptadecane and p-cymene. The combined and screened attractants have a significant attraction effect on leafhoppers, and after the attractants are prepared into the preparation, the preparation can be applied to leafhopper attraction in a field, and the effect is remarkable.

Description

Technical Field

[0001] This invention belongs to the field of pest control technology, specifically relating to a preparation that affects the attraction behavior of leafhoppers and its application in attracting or repelling leafhoppers. Background Technology

[0002] Leafhoppers and other piercing-sucking insects are dominant pests in tea gardens, and their outbreak frequency has been increasing in recent years, seriously affecting the development of the tea industry. Current control measures mainly rely on chemical control; however, excessive use of pesticides has not only led to high levels of insect resistance to various pesticides but also exacerbated environmental pollution, making it highly likely that pesticide residue levels in tea will exceed the maximum residue limits for tea products. Therefore, there is an urgent need to find a new, efficient, safe, and harmless method for controlling leafhoppers. Summary of the Invention

[0003] In view of the problems existing in the prior art, the purpose of this invention is to provide a preparation that affects the attraction behavior of leafhoppers and its application in attracting or avoiding leafhoppers.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] An agent for influencing leafhopper attraction behavior, wherein the active ingredient comprises at least one of acetophenone, terpinene, linalool, ocimene, linalool oxide, α-pinene, sabinene, eucalyptol, heptadecane, and p-cymene; preferably, the active ingredient comprises at least one of acetophenone, terpinene, linalool, sabinene, eucalyptol, heptadecane, and p-cymene; more preferably, the active ingredient is at least two of acetophenone, sabinene, and eucalyptol; wherein the effect on leafhopper attraction behavior is to attract or repel leafhoppers.

[0006] Based on the above scheme, the concentration of the active ingredient in the agent affecting the leafhopper's tactical behavior is 0.001.

[0007] μL / mL~1000μL / mL.

[0008] The above-mentioned agents that affect leafhopper attraction behavior are used in the preparation of leafhopper attractants or leafhopper repellents.

[0009] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol, and juniperene; wherein the volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1, 10:1:1, or 60:1:1, with a working concentration of 0.1 μL / mL when the ratio is 2:1:1, 0.1 μL / mL to 1000 μL / mL when the ratio is 10:1:1, and 1000 μL / mL when the ratio is 60:1:1.

[0010] A leafhopper attractant, the active ingredients of which are acetophenone and juniperene; wherein the volume ratio of acetophenone to juniperene is 1:1, and the working concentration is 1000 μL / mL.

[0011] A leafhopper attractant, the active ingredients of which are eucalyptol and juniperene; wherein the volume ratio of eucalyptol to juniperene is 1:1, and the working concentration is 0.001 μL / mL to 1000 μL / mL.

[0012] A leafhopper repellent, the active ingredients of which are acetophenone, eucalyptol, and juniperene, wherein the volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1 or 60:1:1. When the volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1, the working concentration is 1000 μL / mL. When the volume ratio of acetophenone, eucalyptol, and juniperene is 60:1:1, the working concentration is 0.001 μL / mL.

[0013] The above-mentioned attractants or repellents are used in the control of leafhoppers, and the leafhopper is *Spodoptera litura*.

[0014] The beneficial effects of this invention are as follows:

[0015] This invention provides a formulation that influences leafhopper attraction behavior. The active ingredient comprises at least two of acetophenone, eucalyptol, and juniperene, exhibiting significant attraction or repulsion effects on leafhoppers. When prepared into a formulation, it can be applied in field to attract or repel leafhoppers with remarkable results. Since the active ingredient of this invention is derived from plants, it can reduce pesticide use and has good ecological benefits. Attached Figure Description

[0016] Figure 1 Total ion current chromatogram of volatile components in 'Fuding Dabai' camellia flowers;

[0017] Figure 2 The behavioral response of adult *Spodoptera litura* to a single-component volatile dilution solution was studied (* indicates a significant difference in the number of insects between the tactic treatment and the control (P<0.05; χ2-test); ** indicates an extremely significant difference in the number of insects between the tactic treatment and the control (P<0.01; χ2-test)).

[0018] Figure 3 The study investigated the field effects of 13 components of camellia volatiles on the small green leafhopper in tea gardens (where x-axis 1-14 represent acetophenone, terpinene, phenethyl alcohol, linalool, ocimene, linalool oxide, myrcene, α-pinene, sabinene, eucalyptol, tridecane, heptadecane, p-cymene, and liquid paraffin, respectively. Error bars represent the standard error between replicates; the same letter indicates no significant difference between means (P>0.05), and different letters indicate significant differences between means (P<0.05, one-way ANOVA)).

[0019] Figure 4 The behavioral response of the small green leafhopper to the volatile stock solution of 10 mixed components (* indicates a significant difference in the number of insects between the tactic treatment and the control (P<0.05; χ2-test); ** indicates a highly significant difference in the number of insects between the tactic treatment and the control (P<0.01; χ2-test)).

[0020] Figure 5 The behavioral response of the small green leafhopper to diluted solutions of volatile compounds from four mixed components was studied (* indicates a significant difference in the number of insects between the directional treatment and the control (P<0.05; χ2-test); ** indicates a highly significant difference in the number of insects between the directional treatment and the control (P<0.01; χ2-test)).

[0021] Figure 6 The field regulation effects of 10 mixed formulations on the small green leafhopper in tea gardens were studied (error bars represent standard errors between replicates; the same letter indicates no significant difference between means (P>0.05), and different letters indicate significant differences between means (P<0.05, one-way ANOVA)). Detailed Implementation

[0022] Other terms used in this invention, unless otherwise stated, generally have the meanings commonly understood by those skilled in the art. The invention is further described in detail below with reference to specific embodiments and data. The following embodiments are merely illustrative and are not intended to limit the scope of the invention in any way.

[0023] I. Determination of the composition and release amount of volatile substances from 'Fuding Dabai' camellia flowers

[0024] Volatile compounds from Fuding Dabai tea flowers were collected using dynamic headspace adsorption. The composition and release amount of these volatile compounds were analyzed by GC-MS. Figure 1 Thirteen major volatile compounds were ultimately identified (α-pinene, sapinene, myrcene, p-cymene, eucalyptol, ocimene, terpinene, α-phenylethanol, linalool, acetophenone, linalool oxide, tridecane, and heptadecane, etc.). These compounds were categorized into alcohols, alkanes, ketones, and terpenes. Acetophenone had the highest content in camellia volatiles, with a release rate of 2437.86 ± 320.24 ng / hr (Table 1).

[0025] Table 1. Main components and relative content of volatile substances from 'Fuding Dabai' tea flowers

[0026]

[0027] II. The attraction effects of different camellia volatiles on leafhoppers (indoor behavioral experiment)

[0028] Indoor behavioral tropism experiments were conducted to determine the behavioral responses of the small green leafhopper to three concentration levels of 13 volatile components from camellia.

[0029] A Y-shaped olfactory instrument was used to determine the tropism of adult leafhoppers to three concentrations of 13 compounds. Each compound was diluted in liquid paraffin at concentrations of 10, 0.1, and 0.001 μL / mL. In each experiment, 100 μL of the test compound was dropped onto filter paper as a treatment odor source, and 100 μL of liquid paraffin was used as a control.

[0030] The experiment was conducted in a dark laboratory at 25±2℃ and 70±5% humidity. A glass Y-shaped tube olfactory meter (inner diameter 3cm, diameter length 14cm, arm length 10cm, inner angle 120°) was placed in a dark observation room (80×50×60cm). 3 In the olfactory system, an 18W cool white fluorescent tube is positioned above, providing uniform illumination. One arm is connected to a glass sample bottle containing the test compound, and the other arm is connected to a control sample bottle containing liquid paraffin. An electric vacuum pump is connected to both arms of the "Y"-shaped olfactory system. Inlet air is filtered by activated carbon, flow is controlled by a flow meter, and humidified with absorbent cotton balls. All components are connected by Teflon tubing. The airflow through each arm is maintained at 300 mL / min. Before each test, the airflow is measured using a photometer (TES).

[0031] (1332A, TES Electrical & Electronics Co., Ltd., Taiwan, China) Measure the light intensity on each arm to keep it consistent (approximately 100 lux), and adjust the "Y" tube until the intensity of both arms and the inlet is similar. Before introducing the leafhopper, 1 / 4 (4.5 × 4.5)π cm 2 100 μL of the test compound or control solution (liquid paraffin) was impregnated in a fan-shaped filter paper and placed in a glass odorant bottle connected to the tube arm. Leafhoppers were starved for 2 hours before the olfactory instrument test. Each adult leafhopper was used only once in one test. The behavior of the leafhoppers was then observed, and their selection of the odor from the tube arm of the Y-type olfactory instrument was recorded. When a leafhopper did not reach the marked point within 5 minutes, it was recorded as "no response". Leafhoppers were tested, with 30 leafhoppers tested for each compound. The odorant source was swapped after 5 leafhoppers were tested to eliminate the influence of unforeseen asymmetries in the Y-type tube setup. Each compound odor required 30 leafhoppers to make a successful selection. After one compound was tested, the Y-type olfactory instrument and glass container were rinsed with 98% acetone and then dried in a 100°C oven for 2 hours. Activated charcoal was reactivated at 100°C for 4 hours before use.

[0032] The results are as follows Figure 2As shown, the leafhopper *Symplocos spp.* exhibits positive tropism towards sapinene, heptadecane, linalool, eucalyptol, terpinene, acetophenone, and cymene, but shows no significant tropism towards α-pinene, linalool oxide, myrcene, phenethyl alcohol, ocimene, and tridecane. Among the seven tea plant volatile components to which *Symplocos spp.* exhibits positive tropism, sapinene, heptadecane, and linalool show the strongest attraction. At the three concentration levels measured, the leafhopper showed significant or highly significant positive tropism towards sapinene, heptadecane, and linalool at two different concentration levels, meaning that *Symplocos spp.* selects sapinene (0.1 μL / mL: χ²). 2 =10.221, df=1, P<0.01; 10μL / mL: χ 2 =18.484, df=1, P<0.01), heptadecane (0.001μL / mL: χ 2 =10.221, df=1, P<0.01; 10μL / mL: χ 2 =10.221, df=1, P<0.01) and linalool (0.1μL / mL: χ²) 2 The number of leafhoppers selecting 0.001 μL / mL linalool was significantly higher than that of the control (χ² = 7.521, df = 1, P < 0.01). 2 =5.272, df=1, P=0.022). Among them, the leafhopper showed the highest response rate to 10 μL / mL sapinene, reaching 80%. In addition, the small green leafhopper showed a response rate to a tested concentration of eucalyptol (0.001 μL / mL: χ²=5.272, df=1, P=0.022). 2 =5.272, df=1, P=0.022), terpinene (0.1μL / mL: χ 2 =5.272, df=1, P=0.022), acetophenone (0.1μL / mL: χ²) 2 =5.272, df=1, P=0.022) and for cymene (0.1 μL / mL: χ 2 =5.272, df=1, P=0.022) significantly skewed towards the tested compound compared to the control. Different volatile compounds exhibited varying regulatory activity concentrations on *Symplocos septemlobus*. *Symplocos septemlobus* showed behavioral selectivity towards terpinene, acetophenone, and cymene at a concentration of 0.1 μL / mL, while the lowest concentration (0.001 μL / mL) of eucalyptol showed significant behavioral regulatory activity. *Symplocos septemlobus* showed some negative skewness towards 10 μL / mL α-pinene and 0.001 μL / mL linalool oxide, with a response rate of 63%, but this was not significantly different from the control (χ²=5.272, df=1, P=0.022). 2 =2.93, df=1, P>0.05).

[0033] The above experiments determined the tropism of different compounds on leafhoppers. It was found that compared with the control, *Leymus chinensis* was more inclined to use juniperene (10, 0.1 μL / mL), heptadecane (10, 0.001 μL / mL), linalool (0.1, 0.001 μL / mL), eucalyptol (0.001 μL / mL), terpinene (0.1 μL / mL), acetophenone (0.1 μL / mL), and p-cymene (0.1 μL / mL). This indicates that the camellia volatiles that have a regulatory effect on *Leymus chinensis* have a certain concentration threshold.

[0034] III. The regulatory effects of different camellia volatiles on tea leafhoppers (field trapping experiment)

[0035] Thirteen compounds were diluted with liquid paraffin to prepare four concentrations: 1000, 10, 0.1, and 0.001 μL / mL. For each experiment, 750 μL of each solution was pipetted into a 2 mL slow-release bottle as the treatment odor source, and 750 μL of liquid paraffin served as the control. This experiment was conducted at Shandong Taishan Tea Valley Agricultural Development Co., Ltd., using the 'Fuding Dabai' tea variety. Yellow sticky traps (25 cm × 20 cm) used in the field trapping experiment were purchased from Zhangzhou Yinggeer Agricultural Technology Co., Ltd. In the field experiment, the slow-release bottles were attached to the center of the double-sided yellow sticky traps using fine threads. Each trap was repeated three times using a completely randomized design. These yellow traps were hung on bamboo poles, with the bottom of the traps positioned directly above the tea bush canopy. Treatments were spaced 3 m apart, and a blank control was included. The number of small green leafhoppers on the yellow traps was counted after 3 and 7 days.

[0036] The results are as follows Figure 3As shown, the results of the investigation 3 days after treatment showed that the number of *Symplocos spp.* leafhoppers on sticky traps with 1000 μL / mL ocimene, juniperene, and eucalyptol attractants was significantly different from the control (F = 2.209, df = 13, P < 0.05), with the numbers being 88.33 ± 4.91 per trap, 94 ± 16.86 per trap, and 79.67 ± 6.36 per trap, respectively. At a concentration level of 10 μL / mL, the number of compounds with attractant activity against *Symplocos spp.* increased, including acetophenone, linalool, linalool oxide, α-pinene, and eucalyptol. The number of leafhoppers on sticky boards with linalool, heptadecane, and p-cymene lures was significantly higher than the control (F = 3.184, df = 13, P < 0.05). At a concentration level of 0.1 μL / mL, the number of leafhoppers on sticky boards with lures containing acetophenone, terpinene, linalool, and linalool oxide was significantly higher than the control (F = 4.001, df = 13, P < 0.05). Among these, the linalool lure treatment resulted in the highest number of leafhoppers captured on sticky boards, reaching 107 ± 8.72 leafhoppers / board, which was higher than the number of leafhoppers captured on sticky boards treated with the other three concentrations of compounds. At a concentration level of 0.001 μL / mL, the number of leafhoppers on sticky boards with lures containing linalool and linalool oxide was significantly higher than the control (F = 2.440, df = 13, P < 0.05). In summary, after 3 days of treatment, the number of small green leafhoppers attracted to sticky insect boards with lures containing acetophenone (10, 0.1 μL / mL), terpinene (0.1 μL / mL), linalool (0.1 μL / mL), ocimene (1000 μL / mL), linalool oxide (0.001 μL / mL), juniperene (1000 μL / mL), eucalyptol (1000, 10 μL / mL), and heptadecane (10 μL / mL) differed significantly from the control, with the highest being 107 ± 8.71 insects / board and the lowest being 80.67 ± 6.94 insects / board.

[0037] Over time, high concentrations of compounds generally attracted leafhoppers at 7 days. At a concentration of 1000 μL / mL, the number of compounds active against the small green leafhopper (Ceratophyllum demersum) was greater than at other concentrations, specifically acetophenone, linalool, linalool oxide, α-pinene, sapinene, and eucalyptol. The number of leafhoppers on sticky traps with these lures was significantly higher than the control (F = 4.859, df = 13, P < 0.05). At a concentration of 10 μL / mL, the number of leafhoppers on sticky traps with acetophenone and eucalyptol were significantly different from the control (F = 1). The number of small green leafhoppers on sticky insect boards with acetophenone, terpinene, linalool, and linalool oxide lures was significantly higher than that of the control at a concentration of 0.1 μL / mL (F = 1.975, df = 13, P < 0.05). At a concentration of 0.001 μL / mL, the number of small green leafhoppers on sticky insect boards with lures containing the 13 camellia volatile components was not significantly different from that of the control (F = 0.894, df = 13, P > 0.05). In summary, after 7 days of treatment, the compounds with the highest attraction effects on the small green leafhopper were juniperene (1000 μL / mL), acetophenone (10 μL / mL), eucalyptol (10 μL / mL), and terpinene (0.1 μL / mL), with the highest number of leafhoppers on the sticky boards reaching 246.67 ± 44.83 per board.

[0038] The leafhoppers in the following examples were sourced from Shandong Taishan Tea Valley Agricultural Development Co., Ltd., and the tea tree variety was 'Fuding Dabai'. The reagents acetophenone, eucalyptol, and juniperene used were purchased from Shanghai Maclean Biochemical Co., Ltd.

[0039] Example 1

[0040] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol and juniperene; wherein the volume ratio of acetophenone, eucalyptol and juniperene is 60:1:1.

[0041] Example 2

[0042] A leafhopper attractant, the active ingredients of which are acetophenone and eucalyptol; wherein the volume ratio of acetophenone to eucalyptol is 60:1.

[0043] Example 3

[0044] A leafhopper attractant, the active ingredients of which are acetophenone and juniperene; wherein the volume ratio of acetophenone to juniperene is 60:1.

[0045] Example 4

[0046] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol and juniperene; wherein the volume ratio of acetophenone, eucalyptol and juniperene is 1:1:1.

[0047] Example 5

[0048] A leafhopper attractant, the active ingredients of which are acetophenone and eucalyptol; wherein the volume ratio of acetophenone to eucalyptol is 1:1.

[0049] Example 6

[0050] A leafhopper attractant, the active ingredients of which are acetophenone and juniperene; wherein the volume ratio of acetophenone to juniperene is 1:1.

[0051] Example 7

[0052] A leafhopper attractant, the active ingredients of which are eucalyptol and juniperene; wherein the volume ratio of eucalyptol to juniperene is 1:1.

[0053] Example 8

[0054] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol and juniperene; wherein the volume ratio of acetophenone, eucalyptol and juniperene is 2:1:1.

[0055] Example 9

[0056] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol and juniperene; wherein the volume ratio of acetophenone, eucalyptol and juniperene is 3:1:1.

[0057] Example 10

[0058] A leafhopper attractant, the active ingredients of which are acetophenone, eucalyptol and juniperene; wherein the volume ratio of acetophenone, eucalyptol and juniperene is 10:1:1.

[0059] Effects of different attractants on indoor directional behavior of leafhoppers

[0060] Ten leafhopper attractant stock solutions (prepared by mixing stock solutions of acetophenone, eucalyptol, and juniperene in a specific volume ratio) were prepared according to Examples 1-10 to determine the effect of the attractant stock solutions on the indoor attraction behavior of the small green leafhopper. The determination method was the same as described in (II) The attraction effect of different camellia volatiles on leafhoppers (indoor behavioral experiment).

[0061] The results are as follows Figure 4 As shown, blend1 to blend10 refer to the leafhopper attractants of Examples 1-10, respectively. Figure 4 It can be seen that blend8(χ) 2 =7.521, df=1, P<0.01) showed a highly significant repellent effect on the small green leafhopper, blend1(χ 2 =5.272,df=1,P<0.05), blend 7(χ 2=5.272,df=1,P<0.05), blend 10(χ 2 =5.272, df=1, P<0.05) had a significant attraction effect on the small green leafhopper.

[0062] Based on the above results, the stock solutions of attractants Blend1, Blend7, Blend8, and Blend10, which showed significant selective effects on the leafhopper *Spodoptera litura*, were diluted with liquid paraffin to concentrations of 10 μL / mL, 0.1 μL / mL, and 0.001 μL / mL, respectively. The indoor tactic behavior of the leafhopper was measured at different concentrations of the attractant dilutions. 100 μL / mL refers to the stock solution of the attractant. 100 μL of each solution was dropped onto filter paper and used as an odor source for Y-tube testing, with liquid paraffin serving as a control. The measurement method was the same as described in (II) The Attraction Effect of Different Camellia Volatile Compounds on Leafhoppers (Indoor Behavioral Experiment).

[0063] The results are as follows Figure 5 As shown, the small green leafhopper significantly tends to consume 1000 μL / mL blend1(χ²) 2 =5.272, df=1, P<

[0064] 0.05), while for 0.001 μL / mL blend1(χ 2 =5.272, df=1, P<0.05) showed a significant repulsive response. The leafhopper *Spodoptera exigua* exhibited a significant repulsive response to 1000 μL / mL blend7(χ²) 2 =5.272,df=1,P<0.05), 0.1μL / mL blend7(χ 2 =5.272, df=1, P<0.05) showed significant directional selection, and exhibited a high degree of favoring for 0.001 μL / mL blend7(χ²) 2 =7.521, df=1, P<0.01) showed a highly significant tactic response. Blend8, at high concentrations, exhibited a highly significant repellent effect on leafhoppers (χ² = 7.521, df = 1, P < 0.01). 2 =7521, df=1, P<0.01), but the repulsive effect gradually decreased with decreasing concentration. At 0.1 μL / mL (χ²), 2 At concentrations of 1000 μL / mL (χ²=10.221, df=1, P<0.01), the attractant significantly attracted *Spodoptera litura* leafhoppers, but lower concentrations had no effect. The attractant blend10 attracted leafhoppers at all four concentrations, but primarily at 1000 μL / mL (χ²=10.221, df=1, P<0.01). 2 =5.272, df=1, P<0.05) and 0.1μL / mL (χ 2 When the concentration of df = 5.272 (df = 1, P < 0.05), it significantly attracted the small green leafhopper.

[0065] Effects of different attractants on the field directional behavior of leafhoppers

[0066] Ten leafhopper attractant stock solutions, as described in Examples 1-10, were prepared respectively, and the effects of different attractant stock solutions on the field tactic behavior of leafhoppers were determined.

[0067] In the field trial, 300 μL of the attractant stock solution was placed into a 2 mL volumetric slow-release bottle, and the slow-release bottle was then fixed in the center of a double-sided yellow adhesive plate to conduct a field regulation experiment on the tea leafhopper using the mixed volatile formulation. 300 μL of liquid paraffin was used as a control.

[0068] This experiment was conducted at Shandong Taishan Tea Valley Agricultural Development Co., Ltd., using the tea variety 'Fuding Dabai'. Yellow sticky traps (25cm × 20cm) used in the field trapping experiment were purchased from Zhangzhou Yinggeer Agricultural Technology Co., Ltd. In the field experiment, double-sided yellow sticky traps were used to treat single-component and mixed-component volatile substances. Slow-release bottles were attached to the center of the upper edge of the trap using fine threads. Each trap was repeated three times using a completely randomized design. These yellow traps were hung on bamboo poles, with the bottom of the traps positioned directly above the tea bush canopy. Treatments were spaced 3 meters apart, with a blank control included. The number of small green leafhoppers on the yellow traps was counted after 3 and 7 days.

[0069] The results are as follows Figure 6As shown, after 3 days of treatment, the number of leafhoppers attracted by the blend1 bait sticky board composed of acetophenone, eucalyptol, and juniperene in a 60:1:1 ratio was significantly different from the control (F = 2.682, df = 10, P < 0.05), with a leafhopper count of 206.33 ± 28.83 per board. In both surveys, the number of leafhoppers attracted by the blend6 bait sticky board composed of acetophenone and juniperene in a 1:1 ratio was significantly higher than the control sticky board (3d: F = 2.682, df = 10, P < 0.05; 7d: F = 3.030, df = 10, P < 0.05), with a leafhopper count of 276 ± 24.11 per board after 3 days of treatment and 437.67 ± 44.73 per board after 7 days of treatment. The blend 8 lure, composed of acetophenone, eucalyptol, and juniperene in a 2:1:1 ratio, significantly increased the number of leafhoppers attracted by sticky traps after 3 and 7 days of treatment compared to the control (3d: F = 2.682, df = 10, P < 0.05; 7d: F = 3.030, df = 10, P < 0.05). The leafhopper count on the traps was 213.67 ± 43.97 per trap at 3 days and 427 ± 51.45 per trap at 7 days. The blend 7, composed of eucalyptol and juniperene in a 1:1 ratio, showed enhanced leafhopper attraction after 7 days of treatment, significantly different from the control (F = 3.030, df = 10, P < 0.05). In both surveys, the other five blends did not show significant regulatory effects on the small green leafhopper.

[0070] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A leafhopper attractant, characterized in that, The active ingredients are acetophenone, eucalyptol, and juniperene; the volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1, 10:1:1, or 60:1:1; when the ratio is 2:1:1, the concentration of the active ingredient is 0.1 μL / mL; when the ratio is 10:1:1, the concentration of the active ingredient is 0.1 μL / mL to 1000 μL / mL; and when the ratio is 60:1:1, the concentration of the active ingredient is 1000 μL / mL.

2. A leafhopper repellent, characterized in that, The active ingredients are acetophenone, eucalyptol, and juniperene. The volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1 or 60:1:

1. When the volume ratio of acetophenone, eucalyptol, and juniperene is 2:1:1, the concentration of the active ingredient is 1000 μL / mL. When the volume ratio of acetophenone, eucalyptol, and juniperene is 60:1:1, the concentration of the active ingredient is 0.001 μL / mL.

3. The application of the attractant of claim 1 or the repellent of claim 2 in the control of leafhoppers.

4. The application according to claim 3, characterized in that, The leafhopper in question is the small green leafhopper.

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