A pesticide composition for preventing and treating Momordica grosvenori white rot

Abstract

The invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating Sclerotium rutaecarpa. A pesticide composition for preventing and treating Sclerotium rutaecarpa, wherein the active ingredients are compounded with Ningnanmycin, Fluopyram or Tebuconazole and Piper longum. After compounding, the pesticide composition of the invention has a synergistic effect on Sclerotium rutaecarpa fungi, can improve the prevention and treatment effect, and can also delay the generation and development of drug resistance of pathogenic bacteria.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating Siraitia grosvenori. Background Art

[0002] Momordica grosvenori is a vine plant of the Cucurbitaceae family. It is sweet and cool in nature. In addition to its edible value, it also has great medicinal value. During its growth, Momordica grosvenori is susceptible to diseases such as white rot, root rot, root knot nematode disease and anthracnose. Among them, Sclerotium rolfsii can cause Momordica grosvenori white rot (a soil-borne disease), which mainly harms the base of the stem. The diseased part initially appears as dark brown water-soaked spots. In severe cases, white silky mycelium grows on the surface and spreads radially around the surface. In severe cases, the cortex of the stem rots and the wood is exposed, causing the entire plant to wilt and die.

[0003] At present, the prevention and control of Sclerotium rot in Momordica grosvenori is still mainly chemical agents, such as lime sulfur, flusilazole and mexamethylenetetramine. However, the long-term use of a single variety of chemical agents is likely to cause the emergence and development of disease resistance, resulting in reduced prevention effectiveness, and is also likely to cause environmental pollution and excessive pesticide residues, which is contrary to the requirements of sustainable and healthy agricultural development.

[0004] Piper longum amide (CAS: 20069-09-4) is mainly found in the root of Piper longum, a plant of the Piperaceae family. Its molecular structure is as follows:

[0005]

[0006] Piper longum amide has important medicinal value, including being used to treat viral hepatitis, stomachache, tumors, bronchitis, diarrhea and respiratory tract infections, among which the anti-tumor activity of piper longum amide has been studied most extensively. In addition, the prior art also discloses the use of piper longum amide in agriculture. Patent No. CN113812412A discloses the use of piper longum amide in agricultural weed control herbicides; Patent No. CN113826629A discloses the use of piper longum amide in controlling plant pathogenic fungi; Patent No. CN113796379A discloses the use of piper longum amide in controlling plant bacterial diseases.

[0007] Compounding or mixing different pesticides is an effective way to develop new pesticides and prevent and treat resistant diseases. At present, there are no reports on compounding ningnanmycin, fluopyram or tebuconazole with piperlongum.

[0008] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0009] The purpose of the present invention is to provide a pesticide composition for preventing and controlling Sclerotium rot of Momordica grosvenori, so as to solve the problem that long-term use of a single variety of chemical agents is likely to cause the generation and development of disease resistance, resulting in reduced prevention efficiency, and is also likely to cause environmental pollution and excessive pesticide residues, which is contrary to the requirements for sustainable and healthy development of agriculture.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A pesticide composition for preventing and treating Sclerotium rutaecarpa, wherein the effective ingredients are prepared by compounding ningnanmycin, fluopyram or tebuconazole and piperlongum.

[0012] Preferably, the mass ratio of Ningnanmycin to piperlongum amide is 1-8:20-1.

[0013] Preferably, the mass ratio of fluopyram to piperidine is 1-35:25-1.

[0014] Preferably, the mass ratio of tebuconazole to piperlongum amide is 1-9:9-1.

[0015] The present invention also provides a fungicide, which is prepared from the pesticide composition and other auxiliary components; wherein the other auxiliary components can be selected from one or more of the dispersants, wetting agents, carriers, solvents, binders, disintegrants, defoamers, antifreeze agents and preservatives in the prior art according to different fungicide formulations.

[0016] Preferably, the mass of the pesticide composition accounts for 0.5-80% of the total mass of the fungicide.

[0017] Preferably, the fungicide is in the form of wettable powder, water dispersible granules, suspension or aqueous solution.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The pesticide composition of the present invention has a good control effect on diseases such as Sclerotium rot caused by Sclerotium sclerotiorum.

[0020] (2) The pesticide composition of the present invention can reduce the amount of pesticide used, thereby reducing the cost of prevention and control and pesticide residues, and can also reduce pollution to the environment.

[0021] (3) The pesticide composition of the present invention has a synergistic effect on S. grosvenori after compounding, which can improve the control effect and also delay the generation and development of drug resistance of pathogens. DETAILED DESCRIPTION

[0022] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example :Screening of control agents

[0024] 1. Test strains

[0025] Sclerotium rolfsii was isolated from infected Momordica grosvenori seedlings and stored on PDA medium for later use.

[0026] 2. Test reagents

[0027] 40% Ningnanmycin technical (Sichuan Jinzhu Ecological Agriculture Technology Co., Ltd.), 98% Fluopyram technical (Bayer AG), 97% Tebuconazole technical (Shenyang Kechuang Chemicals Co., Ltd.), 97% Piperidinamide technical (Shanghai Aladdin Biochemical Technology Co., Ltd.).

[0028] After the test agent is prepared into a single-dose stock solution, multiple groups of ratios are set up. Each single-dose stock solution and the ratio mixture are set with 5 mass concentration gradients according to the equal ratio method.

[0029] 3. Test methods

[0030] The mycelium growth rate method (Mu Liyi, 1994) was used. 1 mL of the drug solution was mixed with 9 mL of PDA culture medium melted and cooled to 50-55°C, and poured into a sterile culture dish with a diameter of 9 cm to make a drug-containing plate of the corresponding concentration; and a treatment without drug was set as a blank control, and 8 plates were set for each treatment. A 5 mm diameter test bacteria cake was inoculated in the center of each drug-containing plate and blank control plate, and the plate was covered with a lid and placed at a constant temperature of 27°C for cultivation. When the diameter of the blank control colony reached more than 2 / 3 of the diameter of the culture dish, the colony diameter was measured by the cross method, and the growth inhibition rate of mycelium by different treatments was calculated.

[0031]

[0032] 4. Data Analysis

[0033] DPS software was used for data statistical analysis to obtain the toxicity regression equations of each single agent and mixed agent and their EC values ​​for the tested pathogens. 50The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method. The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 was antagonistic, 80 < CTC < 120 was additive, and CTC ≥ 120 was synergistic.

[0034]

[0035] In the above formula: ATI--the toxicity index of the mixture; S--the EC of the standard agent 50 , the unit is mg / L; M--EC of the mixture 50 , unit is mg / L.

[0036] TTI=TI A ×P A +TI B ×P B

[0037] In the above formula: TTI-theoretical toxicity index of the mixture; TI A --Toxicity index of A agent; P A --The percentage of agent A in the mixture, in percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0038]

[0039] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0040] 5. Measurement results

[0041] The results are shown in Tables 1-3.

[0042] Table 1 Indoor bioactivity determination of Ningnanmycin and piperlongamide against Sclerotium solani

[0043] Drug name and ratio EC50(mg / L) ATI TTI CTC Ningnanmycin 17.329 100.000 -- -- Piper longum 46.573 37.208 -- -- Ningnanmycin 1: Piper longum amide 20 23.447 73.907 40.198 183.856 Ningnanmycin 1: Piper longum amide 10 18.191 95.261 42.917 221.969 Ningnanmycin 1: Piper longum amide 5 25.858 67.016 47.674 140.573 Ningnanmycin 1: Piper longum amide 1 15.339 112.973 68.604 164.674 Ningnanmycin 4: Piper longum amide 1 13.289 130.401 87.442 149.129 Ningnanmycin 8: Piper longum amide 1 10.677 162.302 93.023 174.475

[0044] As shown in Table 1, after Ningnanmycin and piperlongumamide were mixed, the co-toxicity coefficient against Sclerotium rolfsii of Momordica grosvenori was greater than 120 in the mass ratio range of 1-8:20-1, showing a synergistic effect.

[0045] Table 2 Indoor bioactivity determination of fluopyram and piperlongamide against Sclerotium solani

[0046] Drug name and ratio EC50(mg / L) ATI TTI CTC Fluopyram 2.581 100.000 -- -- Piper longum 46.573 5.542 -- -- Fluopyram 1: Piperamide 25 20.232 12.757 9.175 139.043 Fluopyram 1: Piperamide 15 17.672 14.605 11.445 127.605 Fluopyram 1: Piperamide 10 14.184 18.197 14.129 128.789 Fluopyram 1: Piperamide 5 10.078 25.610 21.285 120.321 Fluopyram 1: Piperamide 1 3.599 71.714 52.771 135.898 Fluopyram 5: Piperamide 1 2.437 105.909 84.257 125.697 Fluopyram 10: Piperamide 1 2.294 112.511 91.413 123.080 Fluopyram 15: Piperamide 1 1.851 139.438 94.096 148.187 Fluopyram 25: Piperamide 1 1.174 219.847 96.367 228.135 Fluopyram 35: Piperamide 1 0.695 371.367 97.376 381.374

[0047] As shown in Table 2, after fluopyram and piperlongamide were compounded, the co-toxicity coefficients against Sclerotium rolfsii of Momordica grosvenori were all greater than 120 in the mass ratio range of 1-35:25-1, showing a synergistic effect.

[0048] Table 3 Indoor bioactivity determination of tebuconazole and piperlongamide against Sclerotium solani

[0049] Drug name and ratio EC50(mg / L) ATI TTI CTC Tebuconazole 8.273 100.000 -- -- Piper longum 46.573 17.764 -- -- Tebuconazole 1: Piper longum amide 9 25.340 32.648 25.987 125.631 Tebuconazole 1: Piper longum amide 7 19.144 43.215 28.043 154.101 Tebuconazole 1: Piper longum amide 3 11.780 70.229 38.323 183.258 Tebuconazole 1: Piper longum amide 1 8.819 93.809 58.882 159.317 Tebuconazole 3: Piper longum amide 1 6.975 118.609 79.441 149.305 Tebuconazole 7: Piper longum amide 1 4.725 175.090 89.720 195.151 Tebuconazole 9: Piper longum amide 1 7.107 116.406 91.776 126.837

[0050] As shown in Table 3, after tebuconazole and piperlongumamide were mixed, the co-toxicity coefficients against Sclerotium rolfsii of Momordica grosvenori were all greater than 120 in the mass ratio range of 1-9:9-1, showing a synergistic effect.

[0051] In summary, the combination of Ningnanmycin, Fluopyram or Tebuconazole with Piper longum has a synergistic effect on Sclerotium rolfsii of Momordica grosvenori, which can improve the control effect and delay the generation and development of drug resistance of pathogens.

[0052] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A pesticide composition for preventing and treating Scabies rot, characterized in that: The effective component is prepared by compounding ningnanmycin and piperlongumide, and the mass ratio of ningnanmycin to piperlongumide is 1-8:20-1.

2. A bactericide, characterized in that: The fungicide is prepared from the pesticide composition according to claim 1 and other auxiliary ingredients.

3. The bactericide according to claim 2, characterized in that The mass of the pesticide composition accounts for 0.5-80% of the total mass of the fungicide.

4. The bactericide according to claim 2, characterized in that The fungicide is in the form of wettable powder, water dispersible granules, suspension or aqueous solution.

Images