Method for extracting jasmine flowers by combination of solvents and application thereof

Abstract

The application discloses a method for extracting jasmine flowers by using a combined solvent, which comprises the following steps: preparing the combined solvent, ultrasonic dispersion, constant temperature oscillation extraction, and filtration and centrifugation. The combined solvent is prepared by using a combination of citric acid, ascorbic acid, lactic acid and glycerol, and the weight ratio of the four components is 2:1:1:5. The combined solvent can balance the extraction amount of total flavonoids and total phenols in jasmine dried flowers, and improve the extraction efficiency. The jasmine flower extract prepared by the method has the effects of removing free radicals, inhibiting tyrosinase and elastase activities, delaying skin aging, and whitening, and has no irritation to the skin.

Description

Technical Field

[0001] This invention relates to a method for extracting jasmine flowers using a combination of solvents and its application, specifically relating to the field of separation. Background Technology

[0002] Jasmine (Jasminum sambac (L.) Aiton) has a refreshing aroma and can be used to make essential oils, fragrances, and other products. It is also used to make jasmine tea, which has high medicinal value. In recent years, researchers have increasingly recognized the value of other components of jasmine. Extraction of jasmine flowers has revealed antioxidant, anti-inflammatory, and analgesic effects. However, fresh jasmine flowers have a short flowering period and are difficult to preserve. They need to be extracted and used as soon as possible after picking. If processing is not timely, partial oxidation will occur, affecting the extraction efficiency. Therefore, jasmine flowers are dried after harvesting. However, the content of effective components is reduced when extracting dried jasmine flowers, and the effective components are not easily dissolved, resulting in significant waste. Therefore, developing a high-efficiency jasmine flower extraction method is crucial.

[0003] Chinese invention patent CN113773908A discloses a method for preparing jasmine flower extract with antioxidant activity and its application. This method involves disrupting the original structure of jasmine flower cell walls, separating or removing some lignin, reducing cellulose crystallinity, and increasing the porosity of the jasmine flower substrate. This improves the mass transfer coefficient of the jasmine flower fluid and the accessibility of the substrate, resulting in good antioxidant activity and high extraction efficiency. However, the raw material requires fresh flowers, limiting the availability of raw materials. Chinese invention patent CN113995084A discloses an extraction method for jasmine flower extract and its application. Jasmine flowers are added to an ethanol-water solution, ground into a slurry, extracted using microwaves, followed by immersion extraction, ultrasonic extraction, and separation using macroporous adsorption resin to obtain the jasmine flower extract. This extract exhibits good antioxidant properties and DPPH scavenging effects; however, the extraction process is complex, requiring multiple steps, and has high production costs. Summary of the Invention

[0004] To improve the extraction efficiency of effective components from dried jasmine flowers and to make them skin-friendly for use in cosmetics, the first aspect of this invention provides a method for extracting jasmine flowers using a combination solvent, comprising the following steps:

[0005] (1) Prepare the combined solvent according to the mass ratio, and mix it evenly with a magnetic stirring pot to obtain the combined solvent;

[0006] (2) Crush the jasmine flowers, add them to the combined solvent, stir evenly, and then ultrasonically crush them.

[0007] (3) Place in a constant temperature shaking incubator and shake for 24 hours for extraction;

[0008] (4) Filter the solution through four layers of gauze, centrifuge the filtrate, and take the supernatant to obtain jasmine extract.

[0009] In a preferred embodiment, the combined solvent comprises an acid and an alcohol, wherein the acid is selected from one or more of citric acid, ascorbic acid, malic acid, lactic acid, and tartaric acid.

[0010] In a preferred embodiment, the alcohol solution is selected from one or a combination of several of glycerol, ethylene glycol, 1,4-butanediol, 1,2-propanediol, 1,3-propanediol, and 1,2-butanediol.

[0011] In a preferred embodiment, the combined solvent is a combination of citric acid, ascorbic acid, lactic acid and glycerol in a weight ratio of 2:1:1:(5-10).

[0012] In a preferred embodiment, the weight ratio of citric acid, ascorbic acid, lactic acid and glycerol is 2:1:1:5.

[0013] During the experiment, the applicant unexpectedly discovered that using a combination of citric acid, ascorbic acid, lactic acid, and glycerol as an extraction solvent could increase the extraction yield of total phenols and total flavonoids from jasmine flowers, especially dried jasmine flowers. This is because citric acid, ascorbic acid, and lactic acid act as hydrogen bond donors and glycerol hydrogen bond acceptors, resulting in stronger electrostatic interactions and the formation of specific polar hydrogen bonds between the extraction solvent combination and the dried jasmine flowers. This is more conducive to the interaction between the extraction solvent and the target substances in the dried jasmine flowers, thus facilitating the release of flavonoids and total phenols from the dried jasmine flowers, thereby improving extraction efficiency. Secondly, since citric acid, ascorbic acid, and lactic acid have low viscosity, their combination with glycerol can improve the fluidity of glycerol and enhance electrostatic interactions, thereby increasing electron and mass transfer of total phenols and total flavonoids between the solvent combinations, thus without reducing the extraction effect. Because glycerol contains a large number of hydroxyl groups, it has a high exchange efficiency with flavonoids, but it inhibits the extraction of total phenolic substances. In particular, the extraction rates of total phenols and total flavonoids can be balanced by the weight ratio of citric acid, ascorbic acid, lactic acid and glycerol in 2:1:1:5, so as to achieve the best extraction effect.

[0014] As a preferred embodiment, step 1 specifically involves: preparing the combined solvent according to the mass ratio, mixing it in a magnetic stirring pot, heating it in a water bath to 75-95°C, and dissolving it evenly.

[0015] As a preferred embodiment, step 1 specifically involves: preparing the combined solvent according to the mass ratio, mixing it in a magnetic stirring pot, heating the water bath to 90°C, and dissolving it evenly.

[0016] In a preferred embodiment, the pulverized jasmine flowers in step 2 are first mixed with water and then added to the combined solvent, with the mass ratio of jasmine flowers to water being 1:(0.1-0.6).

[0017] In a preferred embodiment, the pulverized jasmine flowers in step 2 are first mixed with water and then added to the combined solvent, with a mass ratio of jasmine flowers to water of 1:0.4.

[0018] In a preferred embodiment, the weight ratio of jasmine flowers to the combined solvent is 1:(5-50), more preferably, the weight ratio of jasmine flowers to the combined solvent is 1:(10-20), and most preferably, the weight ratio of jasmine flowers to the combined solvent is 1:15.

[0019] In a preferred embodiment, the temperature of the constant temperature shaking incubator in step 3 is 15-35℃, and the shaking frequency is 100-190r / min.

[0020] In a preferred embodiment, the temperature of the constant temperature shaking incubator in step 3 is 20-30℃, and the shaking frequency is 120-170 r / min. Preferably, the temperature of the constant temperature shaking incubator in step 3 is 25℃, and the shaking frequency is 170 r / min.

[0021] In a preferred embodiment, the ultrasonic fragmentation time in step 2 is 0-25 min.

[0022] In a preferred embodiment, the ultrasonic fragmentation time in step 2 is 10-15 minutes. Preferably, the ultrasonic fragmentation time in step 2 is 10 minutes.

[0023] In a preferred embodiment, the centrifugation speed in step 4 is 2000-4000 r / min, and the centrifugation time is 5-15 min.

[0024] In a preferred embodiment, the centrifugation speed in step 4 is 3000 r / min and the centrifugation time is 10 min.

[0025] A second aspect of the present invention provides an application of a method for extracting jasmine flowers using a combination solvent, which is used in the preparation of jasmine flower extracts for use in cosmetics and skincare products.

[0026] Compared with the prior art, the present invention has the following beneficial effects;

[0027] (1) The method for extracting jasmine flowers using the combined solvent of the present invention uses a combination of citric acid, ascorbic acid, lactic acid and glycerol as the combined solvent, and at a weight ratio of 2:1:1:5, the extraction amount of total flavonoids and total phenols in dried jasmine flowers can be balanced, thereby improving the extraction efficiency.

[0028] (2) The method for extracting jasmine flowers using the combined solvent described in this invention employs ultrasonic disruption and constant temperature oscillation extraction. At an oscillation frequency of 120-170 r / min, the jasmine flower extract exhibits good antioxidant activity and also has a certain antibacterial effect. When applied to cosmetics, it can be used as a natural antibacterial agent and can pass the cosmetic preservative test.

[0029] (3) The method of extracting jasmine flowers with combined solvents described in this invention produces jasmine flower extract that has the effect of scavenging free radicals, inhibiting the activity of tyrosinase and elastase, delaying skin aging, whitening effect, and is non-irritating to the skin. Attached Figure Description

[0030] Figure 1 Images of the Escherichia coli inhibition zone test using the combined solvent jasmine extract from Example 1;

[0031] Figure 2 Images of the Staphylococcus aureus inhibition zone test of the combined solvent jasmine extract in Example 1;

[0032] Figure 3 Images of the Bacillus subtilis inhibition zone test of the combined solvent jasmine extract in Example 1;

[0033] Figure 4 Images of the inhibition zone test of Bacillus cereus extract in the combined solvent of Example 1 are shown.

[0034] Figure 5-7 The growth of Aspergillus niger in 1 mL of water after 24, 48, and 72 hours is shown.

[0035] Figure 8-10 The growth of Aspergillus niger after 24, 48, and 72 hours was measured using 1 mL of blank combined solvent.

[0036] Figure 11-13 The growth of Aspergillus niger after 24, 48, and 72 hours was observed using 1 mL of jasmine extract from Example 1.

[0037] Figure 14-16 The growth of Aspergillus niger in 2 mL of water for 24, 48, and 72 hours is shown.

[0038] Figure 17-19 The growth of Aspergillus niger after 24, 48, and 72 hours was measured using 2 mL of blank solvent combination.

[0039] Figure 20-22 The growth of Aspergillus niger after 24, 48, and 72 hours was observed in 2 mL of jasmine extract from Example 1.

[0040] Figure 23 Images of chicken embryos tested during the 0s stimulus in the control group;

[0041] Figure 24 Images of chicken embryos tested during the 180s stimulation of the control group;

[0042] Figure 25 Image of chicken embryo during 0s stimulation in Example 1;

[0043] Figure 26 This is a picture of a chicken embryo under 180s stimulation, as shown in Example 1. Detailed Implementation

[0044] Example 1

[0045] A method for extracting jasmine flowers using a combination of solvents includes the following steps:

[0046] (1) Prepare the combined solvent according to the mass ratio, and mix it evenly with a magnetic stirring pot to obtain 100g of combined solvent;

[0047] (2) Crush the jasmine flowers, add them to the combined solvent, stir evenly, and then ultrasonically crush them.

[0048] (3) Place in a constant temperature shaking incubator and shake for 24 hours for extraction;

[0049] (4) Filter the solution through four layers of gauze, centrifuge the filtrate, and take the supernatant to obtain jasmine extract.

[0050] The combined solvent is a combination of citric acid, ascorbic acid, lactic acid and glycerol in a weight ratio of 2:1:1:5.

[0051] The specific operation of step 1 is as follows: prepare the combined solvent according to the mass ratio, mix it in a magnetic stirring pot, heat the water bath to 90°C, and dissolve it evenly.

[0052] In step 2, the pulverized jasmine flowers are first mixed with water and then added to the combined solvent. The mass ratio of jasmine flowers to water is 1:0.4. The jasmine flowers were purchased from Holiday Gilcon Pharmacy.

[0053] The weight ratio of jasmine flowers to the combined solvent is 1:15.

[0054] The temperature of the constant temperature oscillation incubator in step 3 is 25℃, and the oscillation frequency is 170r / min.

[0055] The ultrasonic fragmentation time in step 2 is 10 minutes.

[0056] In step 4, the centrifugation speed is 3000 r / min and the centrifugation time is 10 min.

[0057] Example 2

[0058] A method for extracting jasmine flowers using a combined solvent is disclosed. The raw materials are the same as in Example 1, except that the combined solvent is a combination of citric acid, ascorbic acid, lactic acid, and glycerol in a weight ratio of 2:1:1:10. The preparation method is the same as in Example 1.

[0059] Example 3

[0060] A method for extracting jasmine flowers using a combined solvent is disclosed. The raw materials are the same as in Example 1, except that the weight ratio of jasmine flowers to the combined solvent is 1:10. The preparation method is the same as in Example 1.

[0061] Example 4

[0062] A method for extracting jasmine flowers using a combined solvent is disclosed. The raw materials are the same as in Example 1, except that in step 2, the pulverized jasmine flowers are first mixed with water before being added to the combined solvent. The mass ratio of jasmine flowers to water is 1:0.1. The preparation method is the same as in Example 1.

[0063] Example 5

[0064] A method for extracting jasmine flowers using a combination solvent is disclosed. The raw materials are the same as in Example 1, except that the temperature of the constant-temperature shaking incubator in step 3 is 15°C. The preparation method is the same as in Example 1.

[0065] Example 6

[0066] A method for extracting jasmine flowers using a combination solvent is disclosed. The raw materials are the same as in Example 1, except that the oscillation frequency of the constant-temperature shaking incubator in step 3 is 100 r / min. The preparation method is the same as in Example 1.

[0067] Example 7

[0068] A method for extracting jasmine flowers using a combined solvent is disclosed. The raw materials are the same as in Example 1, except that the ultrasonic disruption time is 15 minutes. The preparation method is the same as in Example 1.

[0069] Performance testing

[0070] 1. Determination of total phenol content:

[0071] (1) Preparation of gallic acid standard curve: Gallic acid was prepared into a standard solution of 0.1 mg / mL. 0.00 mL, 0.20 mL, 0.40 mL, 0.60 mL, 0.80 mL, 1.00 mL and 1.20 mL of the above standard solution were accurately measured into 10.0 mL stoppered test tubes. 1.00 mL of Folin-Ciocalteu colorimetric reagent was added and mixed well. Then 5.0 mL of 1 mol / L sodium carbonate solution was added and the solution was diluted to the mark with distilled water. The mixture was mixed well and placed at room temperature in the dark for 1 h. The absorbance value at 760 nm was measured. The standard curve was plotted with the gallic acid concentration as the abscissa and the absorbance value as the ordinate.

[0072] (2) Determination of total phenols in the sample: Accurately transfer 1.00 mL of jasmine extract extracted with the combined solvent of Examples 1-7 into a 10.0 mL test tube, and determine the absorbance value according to the method in (1) above. Find the corresponding value in the gallic acid standard curve based on the absorbance value. The total phenol content of the sample is expressed as the relative amount of gallic acid (gGAE / 100g).

[0073] 2. Determination of total flavonoid content:

[0074] (1) Preparation of rutin standard curve: The reference standard rutin was prepared into a standard solution of 1 mg / mL with methanol. 0.0 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, 1.0 mL, and 1.2 mL of the standard solution were accurately pipetted into 10 mL graduated test tubes. 0.3 mL of 5 wt% sodium nitrite was added, and the mixture was shaken and allowed to stand for 6 min. Then, 0.3 mL of 10 wt% aluminum nitrate was added, and the mixture was shaken and allowed to stand for 6 min. 4 mL of 4 wt% sodium hydroxide solution was added, and the volume was adjusted to distilled water. After shaking and standing for 15 min, the absorbance was measured at 510 nm. The standard curve was plotted with rutin concentration as the x-axis and absorbance as the y-axis.

[0075] (2) Determination of total flavonoids in the sample: Accurately transfer 1.0 mL of jasmine extract extracted with the combined solvents of Examples 1-7 into a 10 mL test tube, and determine the absorbance value according to the method in (1) above. Find the corresponding value in the rutin standard curve based on the absorbance value. The total flavonoid content of the sample is expressed as the relative content of rutin (gCAE / 100g).

[0076] The test results for total phenols and total flavonoids are shown in Table 1.

[0077] Table 1

[0078] Total phenol content (gCAE / 100g) Total flavonoid content (gCAE / 100g) Example 1 1.45 0.952 Example 2 0.264 0.155 Example 3 0.386 0.221 Example 4 0.565 0.348 Example 5 0.497 0.522 Example 6 0.518 0.722 Example 7 1.38 0.988

[0079] 3. Antioxidant properties:

[0080] (1) Preparation of standard curve: Take 0.1 mL of ascorbic acid standard solutions of different concentrations (0 mg / mL, 0.2 mg / mL, 0.4 mg / mL, 0.6 mg / mL, 0.8 mg / mL, and 1 mg / mL) into stoppered test tubes, add 5 mL of mixed solution (containing 0.6 mol / L sulfuric acid, 28 mmol / L sodium phosphate, and 4 mmol / L ammonium molybdate) to each tube, stopper the tubes, heat them in a 95℃ water bath for 90 min, remove them and cool them to room temperature, measure the absorbance at a wavelength of 695 nm, and plot the standard curve. The results are shown in Table 2.

[0081] Table 2

[0082]

[0083] The linear equation for the ascorbic acid standard curve was obtained from the data measured in the table:

[0084] Y = 1.3183X + 0.0295R 2 =0.9972

[0085] (2) Determination of antioxidant properties of sample: Take 0.1 mL of jasmine extract extracted with the combined solvent of Example 1 and 0.1 mL of blank combined solvent into a 10 mL test tube, and determine the absorbance value according to the method in (1) above. Find the amount of Vc equivalent to 1 mg of sample on the ascorbic acid standard curve, that is: X mg Vc / mg sample.

[0086] 0.1 mL of jasmine extract extracted with the combined solvent of Example 1 is equivalent to 0.2439 mg Vc / mg sample. (3) DPPH free radical scavenging: Take 0.1 mL of jasmine extract extracted with the combined solvent of Example 1 into a 25 mL test tube, add 3.9 mL of 1×10 -4 A mol / L DPPH solution was added. Timing was started from the addition of the DPPH solution. After incubation at room temperature in the dark for 90 minutes, the absorbance was measured at 517 nm and recorded as A1. Another 0.1 mL blank solvent was used instead of the jasmine extract sample solution, and the absorbance was recorded as A0. The clearance rate was calculated using the following formula:

[0087]

[0088] The DPPH free radical scavenging rate of 0.1 mL of the jasmine flower extract extracted with the combined solvent of Example 1 was measured to be 25.97%.

[0089] (4) Determination of total reducing power: Take 0.2 mL of jasmine extract extracted with the combined solvent of Example 1, add 1 mL of PBS (pH 6.6) and 1 mL of K3[Fe(CN)6] (1 wt%), incubate at 50 °C for 20 min, then rapidly cool, add 1 mL of trichloroacetic acid solution (10 wt%), centrifuge at 3000 r / min for 10 min, take 2 mL of the supernatant, add 2 mL of deionized water and 0.4 mL of FeCl3 solution (0.1 wt%), mix well, let stand for 10 min, and then measure the absorbance at 700 nm. Use Vc and blank combined solvent instead of jasmine extract as controls. Repeat the operation 3 times for each sample. The reducing power is expressed as absorbance value. The test results are shown in Table 3.

[0090] Table 3

[0091]

[0092] The magnitude of absorbance indicates the magnitude of total reducing power. The concentration of Vc and the sample were 0.0302 mg / mL. The comparison of total reducing power was: Vc > Example 1 > Blank.

[0093] 4. Antibacterial effect test: (1) Seed culture preparation: Two loops of colonies were picked from activated Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Bacillus subtilis plates and inoculated into 50 mL of liquid culture medium. The culture was labeled and placed in a constant temperature incubator at 37℃ with shaking at 180 r / min for 15 h. The culture was stopped when the absorbance at 620 nm wavelength on the spectrophotometer reached 0.8.

[0094] Wash the activated Aspergillus niger agar plates with sterile water, collect the bacterial suspension, draw up the bacterial suspension with a syringe and filter the bacterial suspension with a needle filter, take an appropriate amount of filtrate and drop it onto a hemocytometer and place it under an optical microscope to count the number of bacterial spores.

[0095] Cells per milliliter = Average number of cells per small square × 4 × 10 6 × Dilution factor based on spore count 10 5 -10 7 The bacterial solution was used as the seed solution.

[0096] (2) Antibacterial effect test of extract: Filter paper was punched into 0.6 cm diameter discs and placed in a petri dish for sealing and sterilization. Under aseptic conditions, the filter paper discs were immersed in the sample solution for 40 minutes using sterile forceps and then set aside.

[0097] Under aseptic conditions, add 1 mL of bacterial seed culture to every 300 mL of sterilized culture medium cooled to 60°C. Shake thoroughly and pour into a sterile petri dish. After the culture medium has cooled and solidified, place a filter paper soaked in the jasmine extract extracted using the combined solvents of Example 1 onto the solidified test plate (hold it briefly against the edge of the container to filter out excess solution). Press firmly with tweezers to ensure it adheres tightly to the culture medium. Perform two parallel tests for each sample. After standing for 20 minutes, incubate horizontally in a 37°C incubator for 17 hours. Measure the size of the inhibition zone and photograph the results.

[0098] Under aseptic conditions, add 1 mL of pre-counted Aspergillus niger seed solution to every 300 mL of sterilized culture medium cooled to 60°C. After thorough mixing, pour the solution into sterile petri dishes. Place sterilized Oxford cups on a plate containing culture medium and gently press them down to ensure they are in contact with the petri dish without any gaps. Several Oxford cups can be placed on each plate. After cooling and solidification, place the plates flat in a 37°C incubator for 4 days.

[0099] Pour the sterilized Aspergillus niger medium into a sterilized petri dish. After it cools and solidifies, add 1 mL of the jasmine extract extracted with the combined solvent of Example 1 to the plate. Spread the sample evenly with a sterilized spreader. Place the Aspergillus niger medium cylinder cultured in the Oxford cup into the plate with the sample. Let it stand for 20 minutes, then place it flat in a 37°C constant temperature incubator for 3 days. Observe the growth of Aspergillus niger and take pictures to record.

[0100] Immerse the filter paper discs in the jasmine extract extracted using the blank combined solvent and the combined solvent of Example 1 for 40 minutes. When removing them, let them rest briefly at the edge of the container to filter out excess liquid. Place the filter paper discs on the solidified test bacterial plate and press them firmly with tweezers to ensure they adhere well to the culture medium. After standing for 20 minutes, incubate them horizontally in a 37°C incubator for 17 hours. Measure the size of the inhibition zone, photograph and record the results. The control group for Aspergillus niger was prepared using the same inhibition procedure as for Aspergillus niger. The test results for Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Bacillus cereus are shown in Table 4, and the test results for Aspergillus niger are shown in Table 5.

[0101] Table 4

[0102]

[0103] Table 5

[0104]

[0105]

[0106] As shown in Table 3, the jasmine extract extracted by the combined solvent in Example 1 has inhibitory effects on Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Bacillus cereus. Among them, the inhibitory effect on Escherichia coli is the best, and the effect on Bacillus cereus is the least.

[0107] As shown in Table 4, both the blank combined solvent and the jasmine flower extract sample promoted the growth of Aspergillus niger. The growth-promoting effect of the sample and blank solvent at a concentration of 1 mL was stronger than that at a concentration of 2 mL. Based on the colony size measured at different concentrations of water, blank, and sample, the growth-promoting ability of the blank and sample decreased with increasing concentration.

[0108] 5. Free Radical Scavenging Test: The free radical (DPPH) scavenging effect of the jasmine extract extracted with the combined solvent from Example 1 was tested in cosmetics according to "T / SHRH 006-2018 Cosmetics - Experimental Method for Free Radical (DPPH) Scavenging". The control group consisted of a combined solvent solution comprising a combined solvent and water at a volume ratio of 1:1; the combined solvent included citric acid, ascorbic acid, lactic acid, and glycerol at a volume ratio of 2:1:1:5. The experimental group consisted of the jasmine extract from Example 1. The diluent was water. The test results are shown in Table 6.

[0109] 6. Elastase inhibition rate test:

[0110] Solution preparation: Buffer solution: Mix 50 mL of 0.1 mol / L Tris solution (12.114 g / L) with 29.2 mL of 0.1 mol / L hydrochloric acid, then dilute with water to 100 mL to obtain Tris-HCl buffer (pH 8.0); Elastase solution: Add 1 mg of elastase to 1 mL of water to prepare a 30 U / mL stock solution, store at -20°C for later use, and dilute with buffer 10 times to 3 U / mL before use; Substrate solution: Add 1 mg of N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroaniline to 2 mL of water to prepare a substrate solution with a concentration of 0.5 mg / mL.

[0111] Take a 96-well plate, add the reagents according to the steps in the table below and incubate. After incubation, use a microplate reader to measure the absorbance at 410 nm.

[0112]

[0113]

[0114] Inhibition rate (%) = [1 - (A1 - A2) / (B1 - B2)] × 100%

[0115] In the formula, A1, A2, B1, and B2 are the absorbance at 410mm for the corresponding groups.

[0116] The control group was a combined solvent solution comprising a combined solvent and water, with a volume ratio of 1:1; the combined solvent included citric acid, ascorbic acid, lactic acid, and glycerol, with a volume ratio of 2:1:1:5. The experimental group was the jasmine extract of Example 1. The diluent was water. The test results are shown in Table 6.

[0117] 7. Tyrosinase inhibition test: Referring to "T / SHRH 015-2018 Cosmetics - Test Method for Tyrosinase Activity Inhibition", the inhibitory effect of the jasmine extract extracted with the combined solvent in Example 1 on tyrosinase when applied to cosmetics was tested.

[0118] The control group was a combined solvent solution comprising a combined solvent and water, with a volume ratio of 1:1; the combined solvent included citric acid, ascorbic acid, lactic acid, and glycerol, with a volume ratio of 2:1:1:5. The experimental group was the jasmine extract of Example 1. The diluent was water. The test results are shown in Table 6.

[0119] Table 6

[0120]

[0121] 8. Irritation test: Referring to "T / SHRH 011-2018 Cosmetics Eye Irritation Test - Chicken Embryo Chorioallantoic Membrane Test", the irritation of the jasmine extract extracted with the combined solvent in Example 1 on the skin when applied to cosmetics was tested. The control group was a combined solvent solution comprising a combined solvent and water, with a volume ratio of 1:1; the combined solvent included citric acid, ascorbic acid, lactic acid, and glycerol, with a volume ratio of 2:1:1:5. The experimental group was the jasmine extract of Example 1. The diluent was water. The test results are shown in Table 7.

[0122] Scoring (ES) determination. ES ≤ 4 indicates no irritation, 4 < ES ≤ 12 indicates mild irritation, 12 < ES < 16 indicates moderate irritation, and ES ≥ 16 indicates severe irritation.

[0123] Table 7

[0124] control group ES=0 Non-irritating Example 1 ES=0 Non-irritating

[0125] Figure 23 This is a picture of the chicken embryo test at 0 s for the control group, Figure 24 This is a picture of the chicken embryo test at 180 s; Figure 25 This is a picture of the chicken embryo test at 0 s for Example 1, Figure 26 This is a picture of the chicken embryo test at 180 s for Example 1.

Claims

1. A method for extracting jasmine flowers using a combination solvent, characterized in that, Includes the following steps: (1) Prepare the combined solvent according to the mass ratio, and mix it evenly with a magnetic stirring pot to obtain the combined solvent; (2) Crush the jasmine flowers, add them to the combined solvent, stir evenly, and then ultrasonically crush them; (3) Place in a constant temperature shaking incubator and shake for 24 hours for extraction; (4) Filter through four layers of gauze, centrifuge the filtrate, and take the supernatant to obtain jasmine extract; The combined solvent is a combination of citric acid, ascorbic acid, lactic acid and glycerol in a weight ratio of 2:1:1:(5-10).

2. The method for extracting jasmine flowers using a combined solvent according to claim 1, characterized in that, The specific operation of step (1) is as follows: prepare the combined solvent according to the mass ratio, mix it in a magnetic stirring pot, heat the water bath to 75-95℃, and dissolve it evenly.

3. The method for extracting jasmine flowers using a combined solvent according to claim 1, characterized in that, In step (2), the pulverized jasmine flowers are first mixed with water and then added to the combined solvent. The mass ratio of jasmine flowers to water is 1:(0.1-0.6).

4. The method for extracting jasmine flowers using a combined solvent according to claim 1, characterized in that, The temperature of the constant temperature oscillation incubator in step (3) is 15-35℃, and the oscillation frequency is 100-190r / min.

5. The method for extracting jasmine flowers using a combined solvent according to claim 1, characterized in that, The ultrasonic fragmentation time in step (2) is 0-25 min.

6. The method for extracting jasmine flowers using a combined solvent according to claim 1, characterized in that, The centrifugation speed in step (4) is 2000-4000 r / min, and the centrifugation time is 5-15 min.

7. An application of a method for extracting jasmine flowers using a combined solvent according to any one of claims 1-6, characterized in that, It is used in the preparation of jasmine flower extract, which is used in cosmetics and skin care products.

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