Method for growing mycelium, mycelium and applications

Abstract

This invention belongs to the field of mycelium technology, and discloses a method for mycelium growth, mycelium, and its applications. The method includes: immersing a porous material in a nutrient solution until fully saturated, then removing and drying the porous material; inoculating Ganoderma lucidum spawn onto the macropore walls of the dried porous material for cultivation, obtaining Ganoderma lucidum spawn cultured on the porous material; placing the cultured Ganoderma lucidum spawn on the porous material at the bottom of a container containing nutrient solution, ensuring partial immersion of the porous material in the nutrient solution, and cultivating it in a sealed space. The porous material used in this invention has a uniform structure, which is conducive to uniform mycelial growth. The porous material also possesses a certain strength and a smooth surface, facilitating the separation of mycelium from the substrate, resulting in highly active mycelium.

Description

Technical Field

[0001] This invention relates to the field of mycelium technology, specifically to mycelium growth methods, mycelium, and their applications. Background Technology

[0002] Currently, the main method for mycelial growth is solid-state culture. However, due to the unevenness of the biomass particles and internal pores in solid culture media, the resulting mycelial morphology is uneven, and the grown mycelia are not easily detached from the substrate. Furthermore, the rate at which Ganoderma lucidum mycelia digest solid nutrients during growth is relatively slower compared to liquid nutrient solutions. Solid-state culture requires controlled and stable humidity, which increases costs. It is also difficult to change the nutrient composition of solid culture media during mycelial growth; as the mycelia grow, the nutrient composition changes, which is detrimental to subsequent growth.

[0003] Currently, some researchers use liquid culture methods, culturing Ganoderma lucidum mycelia in fermentation broth to obtain mycelial gel materials. However, the mycelia cultured using this method are highly hydrophilic, resulting in brittle materials after drying. In contrast, aerial mycelia obtained through solid-state culture have a high content of hydrophobic proteins, leading to weaker hydrogen bonding between mycelia after drying, which improves the flexibility of the mycelial material. Furthermore, the mycelia in liquid fermentation broth are shorter and thicker compared to those obtained through solid-state fermentation, resulting in inferior strength and flexibility of the dried material.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] To address the problems in the background art, the present invention provides a method for mycelial growth, mycelium, and its applications.

[0006] This growth method allows for faster mycelial growth, resulting in more active mycelial material. No additional humidity control is required during the growth process, allowing for continuous growth and regular harvesting of mycelial material.

[0007] To achieve the above objectives, the first technical solution adopted by the present invention is as follows:

[0008] Methods for mycelial growth include:

[0009] The porous material was immersed in the nutrient solution and fully moistened before being removed and dried.

[0010] The pretreated Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material.

[0011] The Ganoderma lucidum spawn cultured on porous material is placed at the bottom of a container containing nutrient solution, so that the porous material is partially immersed in the nutrient solution, and cultured in a sealed space.

[0012] Preferably, the porous material is a hydrophilic material with a regular vertical pore structure, the pore walls being composed of numerous continuous small pores, the diameter of the regular vertical pores being 2 mm-5 mm, and the diameter of the small pores being 10 μm-300 μm.

[0013] Preferably, the pretreated Ganoderma lucidum strain refers to Ganoderma lucidum spawn balls prepared by liquid fermentation.

[0014] Preferably, the nutrient solution consists of 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 g vitamin B, and 0.1-0.5 g MgSO4, and 1 L deionized water.

[0015] Preferably, the Ganoderma lucidum spawn cultured on porous material is placed at the bottom of a container containing nutrient solution, such that 10%-50% of the height of the porous material is submerged in the nutrient solution.

[0016] Preferably, the container containing the nutrient solution has an opening at the top.

[0017] Preferably, when culturing in a closed space, the temperature of the closed space is 23-32 ℃, the carbon dioxide concentration is 3%-15%, and the culturing time is 7-21 days.

[0018] The second technical solution of this application is: mycelium obtained by any of the above-described growth methods.

[0019] The third technical solution of this application is: the application of the mycelium described above as a mycelium bio-based material.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] This invention utilizes a specific porous material to inoculate Ganoderma lucidum spores onto the macropore walls of the material. The porous material, through its micropores, uses capillary action to continuously transport nutrients from the nutrient solution in the container to the mycelium. Simultaneously, it maintains the necessary nutrient solution composition and oxygen content for growth, fully satisfying the mycelial needs and ensuring mycelial activity. This invention eliminates the need for additional humidity control. The stable liquid culture medium and its nutrients promote faster mycelial growth and higher mycelial activity. The uniform structure of the porous material facilitates even mycelial growth, and its strength and smooth surface promote separation of mycelium from the substrate. Furthermore, this invention allows for continuous growth and periodic harvesting of mycelial material without the need for re-preparation of the culture medium. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments. It should be understood that these descriptions are merely exemplary and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and techniques are omitted in the following description to avoid unnecessarily obscuring the concept of the invention. Unless otherwise specified, specific conditions in the embodiments are performed under conventional conditions or conditions recommended by the manufacturer. Reagents or instruments used, unless otherwise specified, are all commercially available conventional products.

[0023] The present invention provides a method for mycelial growth, comprising: immersing a porous material in a nutrient solution, and after fully immersing it, removing the porous material and drying it; inoculating Ganoderma lucidum spawn onto the macropore walls of the dried porous material for cultivation, thereby obtaining Ganoderma lucidum spawn cultured on the porous material; placing the Ganoderma lucidum spawn cultured on the porous material at the bottom of a container containing nutrient solution, so that the porous material is partially immersed in the nutrient solution, and cultivating it in a sealed space.

[0024] It should be noted that the nutrient solution is a liquid culture medium, and during the cultivation process, the porous material inoculated with Ganoderma lucidum is partially immersed in the liquid culture medium.

[0025] This invention utilizes a specific porous material to inoculate Ganoderma lucidum spores onto the macropore walls of the material. The porous material, through its pores, uses capillary action to continuously transport nutrients from the nutrient solution in the container to the mycelium. Simultaneously, it maintains the required nutrient solution composition and oxygen content for growth, fully satisfying the mycelial needs and ensuring mycelial activity. This invention eliminates the need for additional humidity control, ensures stable liquid and nutrient levels in the culture medium, promotes faster mycelial growth, and results in high mycelial activity. The uniform structure of the porous material facilitates even mycelial growth, and its strength and smooth surface promote separation of mycelium from the substrate.

[0026] In this embodiment of the invention, Ganoderma lucidum spawn balls adhere to the macropore walls of a porous material. Part of the spawn ball comes into contact with the nutrient solution through the micropores of the porous material, while the other part of the spawn ball is exposed outside the liquid surface. Through continuous growth, aerial hyphae gradually fill the macropores. Due to respiration, an oxygen concentration gradient is formed from bottom to top. In order to better absorb oxygen, the hyphae grow upwards, eventually forming a certain height of hyphal foam on the porous material, which can then be harvested.

[0027] The porous material is a hydrophilic material with a regular vertical pore structure. The pore walls are composed of numerous continuous small pores, and these regular vertical pores are macropores. These macropores are used to support mycelial spheres. After absorbing water, the macropores will not become clogged, ensuring a sufficient oxygen supply to the mycelial spheres. The small pores on the pore walls are for delivering nutrients to the mycelium supported on the macropores through capillary action. Therefore, the pore sizes of the macropores and micropores only need to meet the above conditions. In a preferred embodiment, the macropore diameter is 2 mm-5 mm, and the micropore diameter is 10 μm-300 μm.

[0028] Regarding the thickness of the porous material, during cultivation, the porous material needs to be partially immersed in the nutrient solution to better transport nutrients. Therefore, if the porous material is too thin, the portion not immersed in the nutrient solution can only support a small amount of mycelium, and a good oxygen concentration gradient cannot be formed during growth. Those skilled in the art can adjust the thickness of the porous material according to the actual situation. In some preferred embodiments, the thickness of the porous material is 2 cm-10 cm.

[0029] The nutrients are common components of liquid culture media and are not specifically limited. Those skilled in the art can adjust the distribution ratio as needed. In a preferred embodiment, the nutrient solution consists of 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 g vitamin B, and 0.1-0.5 g MgSO4, along with 1 L of deionized water.

[0030] The container has an opening at the top, allowing the Ganoderma lucidum spawn cultured on the porous material to be placed at the bottom of the container containing nutrient solution, ensuring that the porous material is partially immersed in the solution. If the nutrient solution level is too low, it will affect the capillary action of the porous material; if the nutrient solution level is too high, it will submerge a significant portion of the porous material, leading to oxygen deficiency within the macropores and hindering the growth of aerial mycelia. In a preferred embodiment, 10%-50% of the height of the porous material is immersed in the nutrient solution.

[0031] The parameters for cultivation in a closed space do not need to be specifically limited; they are commonly used parameters in mycelial cultivation, and those skilled in the art can adjust them according to actual needs. In a preferred embodiment, the temperature in the closed space is controlled at 23-32 ℃, the carbon dioxide concentration at 3%-15%, and the cultivation period is 7-21 days. After cultivation, the Ganoderma lucidum mycelium growing on the porous material can be harvested with a knife.

[0032] In this embodiment of the invention, liquid culture medium is used as a nutrient source to cultivate mycelium, and solid porous material is used as a growth carrier for mycelium. During the growth process, the mycelium is loaded on the porous material and grows upward to a certain thickness by absorbing nutrients from the liquid nutrient solution, making it easy to harvest clean mycelium material.

[0033] The harvested Ganoderma lucidum mycelium is dried to obtain Ganoderma lucidum mycelium bio-based material, which has significantly improved strength and flexibility.

[0034] To make the technical solution of the present invention clearer, the following examples illustrate the growth method, application and performance of mycelium.

[0035] Example 1

[0036] A porous material is provided, featuring a regular vertical pore structure. The pore walls are composed of numerous continuous small pores, with the large pores having a diameter of 2 mm, the small pores having a diameter of 50 μm, and a thickness of 5 cm.

[0037] The porous material was immersed in a solution consisting of 15 g D-glucose, 2.5 g peptone, 3 g yeast extract, 0.1 g KH2PO4, 0.2 g K2HPO4, 0.1 g vitamin B, 0.1 g MgSO4, and 1 L deionized water. After thorough immersion, the material was removed and dried.

[0038] The liquid-fermented Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material.

[0039] The Ganoderma lucidum culture cultured on the above porous material was placed at the bottom of a container containing nutrient solution. The nutrient solution consisted of 15 g D-glucose, 2.5 g peptone, 3 g yeast extract, 0.1 g KH2PO4, 0.2 g K2HPO4, 0.1 g vitamin B, and 0.1 g MgSO4, along with 1 L of deionized water. The height of the solution was controlled to be 5 mm.

[0040] Place the container in a sealed space, control the temperature in the sealed space to be 23 ℃ and the carbon dioxide concentration to be 3%, and cultivate for 14 days. Then, harvest the Ganoderma lucidum mycelium growing on the porous material with a knife.

[0041] The harvested Ganoderma lucidum mycelium was dried at 40 °C and then pressed under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material.

[0042] Example 2

[0043] A porous material is provided, which has a regular vertical pore structure. The pore walls are composed of countless continuous small pores. The pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 μm, and the thickness is 2 cm.

[0044] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, 0.2 MgSO4, and 1 L deionized water. After thorough immersion, the material was removed and dried.

[0045] The liquid-fermented Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material.

[0046] The Ganoderma lucidum culture cultured on the above porous material was placed at the bottom of a container containing nutrient solution. The nutrient solution consisted of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, and 0.2 g MgSO4, along with 1 L of deionized water. The height of the solution was controlled to be 2 mm.

[0047] Place the container in a sealed space, control the temperature in the sealed space to be 26 ℃ and the carbon dioxide concentration to be 5%, and cultivate for 14 days. Then, harvest the Ganoderma lucidum mycelium growing on the porous material with a knife.

[0048] The Ganoderma lucidum mycelium obtained from the above harvest is dried at 40 ℃ and then pressed under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material.

[0049] Example 3

[0050] A porous material is provided, which has a regular vertical pore structure. The pore walls are composed of countless continuous small pores. The pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 μm, and the thickness is 2 cm.

[0051] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, 0.2 MgSO4, and 1 L deionized water. After thorough immersion, the material was removed and dried.

[0052] The liquid-fermented Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material.

[0053] The Ganoderma lucidum culture cultured on the above porous material was placed at the bottom of a container containing nutrient solution. The nutrient solution consisted of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, and 0.2 g MgSO4, along with 1 L of deionized water. The height of the solution was controlled to be 2 mm.

[0054] Place the container in a sealed space, control the temperature in the sealed space to be 26 ℃ and the carbon dioxide concentration to be 5%, and cultivate for 14 days. Then, harvest the Ganoderma lucidum mycelium growing on the porous material with a knife to complete the first harvest.

[0055] The Ganoderma lucidum mycelium obtained from the first harvest is dried to obtain Ganoderma lucidum mycelium bio-based material.

[0056] Drain the mixed solution added to the container, reconstitute the same nutrient solution, and introduce it into the container, controlling the solution height to 2 mm.

[0057] Place the container with the replaced nutrient solution into a sealed space, control the temperature in the sealed space at 26 ℃ and the carbon dioxide concentration at 5%, and cultivate for 14 days. Then, use a knife to harvest the Ganoderma lucidum mycelium growing on the porous material to complete the second harvest.

[0058] The Ganoderma lucidum mycelium obtained from the second harvest is dried at 40 °C and then pressed under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material. It can be seen that this invention enables regular harvesting of the mycelium by periodically changing the nutrient solution.

[0059] Example 4

[0060] A porous material is provided, which has a regular vertical pore structure. The pore walls are composed of countless continuous small pores. The pore diameter of the large pore is 3 mm, the pore diameter of the small pore is 150 μm, and the thickness is 5 cm.

[0061] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, 0.2 MgSO4, and 1 L deionized water. After thorough immersion, the material was removed and dried.

[0062] The liquid-fermented Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material.

[0063] The Ganoderma lucidum culture cultured on the above porous material was placed at the bottom of a container containing nutrient solution. The nutrient solution consisted of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B, and 0.2 g MgSO4, along with 1 L of deionized water. The height of the solution was controlled to be 10 mm.

[0064] Place the container in a sealed space, control the temperature in the sealed space to be 26 ℃ and the carbon dioxide concentration to be 5%, and cultivate for 14 days. Then, harvest the Ganoderma lucidum mycelium growing on the porous material with a knife.

[0065] The Ganoderma lucidum mycelium obtained from the above harvest is dried at 40 ℃ and then pressed under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material.

[0066] Comparative Example 1

[0067] Compared to Example 1, the only difference is that the macropore diameter of the porous material is adjusted to 0.3 mm; all other components and steps are the same as in Example 1. That is, the porous material in this comparative example is a porous material with a regular vertical pore structure, the pore walls being composed of countless continuous small pores. The macropore diameter is 0.3 mm, the small pore diameter is 50 μm, and the thickness is 5 cm.

[0068] Comparative Example 2

[0069] Compared to Example 1, the only difference is that the macropore diameter of the porous material is adjusted to 10 mm; all other components and steps are the same as in Example 1. That is, the porous material in this comparative example is a porous material with a regular vertical pore structure, the pore walls being composed of countless continuous small pores. The macropore diameter is 10 mm, the small pore diameter is 50 μm, and the thickness is 5 cm.

[0070] Comparative Example 3

[0071] Compared to Example 1, the only difference is that the pore size of the porous material is adjusted to 1 μm; all other components and steps are the same as in Example 1. That is, the porous material in this comparative example is a porous material with a regular vertical pore structure, and the pore walls are composed of countless continuous small pores. The macropore diameter is 2 mm, the micropore diameter is 1 μm, and the thickness is 5 cm.

[0072] Comparative Example 4

[0073] Compared to Example 1, the only difference is that the pore size of the porous material is adjusted to 500 μm; all other components and steps are the same as in Example 1. That is, the porous material in this comparative example is a porous material with a regular vertical pore structure, and the pore walls are composed of countless continuous small pores. The pore diameter is 2 mm, the pore diameter is 500 μm, and the thickness is 5 cm.

[0074] Comparative Example 5

[0075] Compared with Example 2, the only difference is that the thickness of the porous material is adjusted to 0.2 cm, while the other components and steps are the same as in Example 2. That is, the porous material in this comparative example has a regular vertical pore structure, the pore wall is composed of numerous continuous small pores, the diameter of the large pore is 5 mm, the diameter of the small pore is 300 μm, and the thickness is 0.2 cm.

[0076] Comparative Example 6

[0077] Compared with Example 2, the only difference is that the height of the solution is controlled at 14 mm; all other components and steps are the same as in Example 2. That is, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of numerous continuous small pores. The diameter of the large pores is 5 mm, the diameter of the small pores is 300 μm, and the thickness is 2 cm.

[0078] Comparative Example 7

[0079] Compared with Example 2, the only difference is that the height of the solution is controlled at 0.5 mm; all other components and steps are the same as in Example 2. That is, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of numerous continuous small pores. The diameter of the large pores is 5 mm, the diameter of the small pores is 300 μm, and the thickness is 2 cm.

[0080] Comparative Example 8

[0081] Compared with Example 2, the only difference is that the pore diameter of the porous material is 5 mm, while the other components and steps are the same as in Example 2. That is, the porous material in this comparative example has a regular vertical pore structure, the pore wall is composed of numerous continuous uniform pores, the pore diameter is 5 mm, and the thickness is 2 cm.

[0082] Comparative Example 9

[0083] Compared with Example 2, the only difference is that the pore size of the porous material is 300 μm, while the other components and steps are the same as in Example 2. That is, the porous material in this comparative example has a regular vertical pore structure, the pore wall is composed of numerous continuous uniform pores, the pore size is 300 μm, and the thickness is 2 cm.

[0084] Comparative Example 10

[0085] Compared with Example 2, the only difference is that the porous material is omitted, and the Ganoderma lucidum inoculum is directly inoculated into the liquid culture medium for cultivation, as follows:

[0086] S1: Prepare a liquid culture medium consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 g MgSO4, and 1 L deionized water.

[0087] S2: Inoculate the liquid-fermented Ganoderma lucidum strain into the culture medium in step 1 for cultivation.

[0088] S3: Place the Ganoderma lucidum spawn cultured in step 2 into an open mold, and place the mold in a sealed space. Control the temperature in the sealed space to be 26 ℃ and the carbon dioxide concentration to be 5%. Culture for 14 days.

[0089] S4: The Ganoderma lucidum mycelium material obtained in step 3 is dried at 40 ℃ and then pressed under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material.

[0090] Comparative Example 11

[0091] Compared to Example 2, the difference lies in the elimination of the use of porous materials and the replacement of the liquid culture medium with the existing solid culture medium, as detailed below:

[0092] S1: Prepare solid culture medium: 78 g poplar powder, 20 g wheat bran, 1 g calcium sulfate, 1 g sucrose, with a moisture content of 65%.

[0093] S2: Sterilize 100 g of culture medium in step 1 at 121 °C for 30 minutes, then cool to room temperature for later use.

[0094] S3: Inoculate 10 ml of white rot fungal mycelium liquid culture into the mixture in step 2, incubate at 24 ℃ for 3 days, then break it up, put it into a 21 cm * 15 cm * 4 cm plastic dish, flatten it, and incubate for 4 days.

[0095] S4: Place the mixture of biomass pellets and mycelium from step 3 into an open mold, and place the mold in a sealed space. Control the temperature in the sealed space to be 26 ℃ and the carbon dioxide concentration to be 5%. Incubate for 14 days.

[0096] S5: Dry the Ganoderma lucidum mycelium material obtained in step 4 at 40 ℃, and then press it under a pressure of 0.2 MPa for 5 hours to obtain Ganoderma lucidum mycelium bio-based material.

[0097] Experimental Example

[0098] The performance of the Ganoderma lucidum mycelium bio-based materials obtained in Examples 1-4 and Comparative Examples 1-11 was tested.

[0099] Mechanical property testing: Tensile strength and elongation at break were tested using an Instron 5969 electronic universal testing machine with a 500 N sensor. The testing environment was room temperature of 23 ℃ and average humidity of 50%. Each sample was tested five times and the average value was taken.

[0100] Contact angle test: The water contact angle is measured using a contact angle measuring instrument (Krüss, DSA30, Germany).

[0101] The test results are shown in Table 1.

[0102] Table 1

[0103]

[0104]

[0105] Note: "-" in the table indicates that mycelial material could not be obtained under these conditions, and the data test could not be performed.

[0106] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for mycelial growth, characterized in that, include: The porous material was immersed in the nutrient solution and fully moistened before being removed and dried. The pretreated Ganoderma lucidum strain was inoculated onto the macroporous wall of the dried porous material and cultured to obtain the Ganoderma lucidum strain cultured in the porous material. The Ganoderma lucidum spawn cultured on porous material is placed at the bottom of a container containing nutrient solution, with 10%-50% of the height of the porous material immersed in the nutrient solution, and cultured in a sealed space. The porous material is a hydrophilic material with a regular vertical pore structure. The pore walls are composed of numerous continuous small pores. The diameter of the regular vertical pores is 2 mm-5 mm, and the diameter of the small pores is 10 μm-300 μm.

2. The growth method of claim 1, wherein, The pretreated Ganoderma lucidum strain refers to Ganoderma lucidum spawn balls prepared by liquid fermentation.

3. The growth method of claim 1, wherein, The nutrient solution consists of 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 g vitamin B, and 0.1-0.5 g MgSO4, plus 1 L deionized water.

4. The method of claim 1, wherein, The container containing the nutrient solution has an opening at the top.

5. The method as described in claim 1, characterized in that, When culturing in a closed space, the temperature of the closed space is 23-32℃, the carbon dioxide concentration is 3%-15%, and the culturing time is 7-21 days.

6. The application of the mycelial growth method as described in any one of claims 1-5 in the preparation of mycelial bio-based materials.

Images