A plant-derived herbicidal composition and use thereof

Abstract

The present application belongs to the field of pesticides, and particularly relates to a plant source weeding composition, which comprises active ingredient A and active ingredient B; the active ingredient A is paeonol or artesunate, the active ingredient B is 7-ethyl camptothecin, and the mass ratio of the active ingredient A to the active ingredient B is 1:10-10:1. The plant source weeding composition has excellent weeding activity on barnyard grass, sedge and bidens pilosa, and can be directly developed into a plant source weeding active ingredient composition and used for controlling weeds of the families of Poaceae, sedge and broad-leaved weeds in farmland.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of pesticides, and particularly relates to a plant source herbicidal composition and application thereof. BACKGROUND

[0002] The idea of sustainable agricultural development is gradually valued, and biopesticides become a hot spot in current pesticide research and development because of their environmental friendliness, and are an effective strategy for solving the conflict between agricultural development and environmental protection.

[0003] Most plant source herbicidal active compounds are used as lead compounds for structure optimization to develop herbicide products with higher activity, such as herbicides based on plant source herbicidal active ingredients, such as cycloheptenyl ether, diphenyl ether and triketone, which have good commercial value. Our idea is to explore plant source herbicidal active ingredients with higher activity, to develop plant source herbicide products directly by synergistic effect and expansion of herbicidal spectrum. Based on this, we have found that 7-ethyl camptothecin, artemisinin and paeonol have high herbicidal activity, and further developed the combination of 7-ethyl camptothecin and artemisinin, and the combination of 7-ethyl camptothecin and paeonol, to realize the synergistic effect of the two, and have excellent herbicidal activity on barnyard grass, sedge and bidens pilosa, which can be directly developed into plant source herbicidal active ingredient compositions for control of gramineae, sedge and broadleaf weeds. SUMMARY

[0004] The purpose of the present application is to provide a plant source herbicidal composition with synergistic effect and application thereof, and the specific scheme is as follows:

[0005] A plant source herbicidal composition comprises active ingredient A and active ingredient B; the active ingredient A is paeonol or artemisinin, the active ingredient B is 7-ethyl camptothecin, and the mass ratio of the active ingredient A to the active ingredient B is 1:10-10:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:6-6:1, 1:3-3:1 or 1:1. Preferably, the mass ratio of paeonol to 7-ethyl camptothecin is 6:1-1:3. The mass ratio of artemisinin to 7-ethyl camptothecin is 1:3-1:1.

[0006] The plant source herbicidal composition described in the present application further comprises an auxiliary agent, and the weight percentage content of the active ingredient is 1-50%. Preferably, the weight percentage content of the active ingredient is 1-25%.

[0007] The plant source herbicidal composition described in the present application has a dosage form of wettable powder, microemulsion, water suspension, oil suspension and emulsifiable concentrate.

[0008] The plant source weeding composition of the present application is used for preventing and removing crop weeds, and the weeds are grasses, sedge and broadleaf weeds.

[0009] By adopting the technical scheme, the present application has the following beneficial effects:

[0010] The combination of 7-ethyl camptothecin and artesunate and the combination of 7-ethyl camptothecin and paeonol realize the synergistic effect of the two, and have excellent weeding activity on barnyard grass, sedge and bidens pilosa, and can be directly developed into a plant source weeding active ingredient composition and used for preventing and controlling grasses, sedge and broadleaf weeds in farmland. DETAILED DESCRIPTION

[0011] Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0012] The present application will be further explained and described in combination with specific embodiments.

[0013] Example 1: Biological activity determination of the plant source weeding composition on different types of weeds

[0014] 1. Materials and methods

[0015] 1.1 Test agent

[0016] Paeonol, artesunate, 7-ethyl camptothecin, propachlor, Tween 80 and dimethyl sulfoxide.

[0017] 1.2 Test weeds

[0018] Grasses barnyard grass, sedge Cyperus rotundus and broadleaf weeds Bidens pilosa.

[0019] 1.3 Pre-emergence treatment of the combined agent

[0020] The seeds of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa were evenly spread on moist filter paper and germinated at 28℃. 20 seeds of each of the three paddy weeds with uniform germination were selected and evenly spread on a culture dish. Paeonol and 7-ethyl camptothecin, and artesunate and 7-ethyl camptothecin were compounded in the corresponding proportions (Table 1), and then a certain amount of the compounded composition was weighed, dissolved in 15ul dimethyl sulfoxide and 0.1g Tween 80, and made up to 50ml in a 50ml volumetric flask to prepare a 50mg / L stock solution (containing 0.2% Tween 80). The stock solution was diluted with 0.2% Tween water to 25, 12.5, 3.125, 0.78125, 0.19531mg / L, etc. 3ml of the germinated seeds of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa were treated, and then the seeds of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa were placed in an artificial climate chamber for culture (14h of light at 28℃ and 80% RH, 10h of darkness at 25℃ and 75% RH). Each treatment was repeated 3 times. After 7 days of treatment, the inhibition rates of the germination of Echinochloa crus-galli, Cyperus difformis and Bidens pilosa were evaluated.

[0021] Table 1 Binary compounding ratio

[0022]

[0023] 2、Data analysis

[0024] EC 50 SigmaPlot 13.0 software was used for processing and analysis, and the non-linear fitting equation was y = min + (max-min) / [1 + (x / EC 50 ) -Hillslope ], and t-test (P < 0.05) was used for significance analysis.

[0025] SR was used to determine the joint toxicity. EC 50 (theoretical value) and SR (synergistic coefficient) were calculated, and the specific algorithm was as follows:

[0026] EC 50 (theoretical value) = (A + B) / (A / EC 50a + EC 50b )

[0027] SR = EC 50 (theoretical value) / EC 50 (measured value)

[0028] In this experiment, SR between 0.5 and 1.5 was identified as additive effect; SR ≤ 0.5 was identified as antagonism; SR ≥ 1.5 was identified as synergistic effect, wherein A and B represent the proportion of the two drugs in the mixture, and a and b represent the two drugs.

[0029] 3、Test results

[0030] 3.1 Inhibition of C. rotundus shoot and root growth by the combination of the three plant-derived components

[0031] The three plant-derived components, paeonol, 7-ethyl camptothecin and artesunate, had significantly better inhibitory effects on C. rotundus than the control herbicide, pretilachlor. The combination of the three plant-derived components had a significant synergistic effect on the inhibition of C. rotundus.

[0032] The EC50 values for the inhibition of C. rotundus shoot growth were compared (Table 2). 50 The results (Table 2) showed that the combinations of paeonol and 7-ethyl camptothecin (1:3), artesunate and 7-ethyl camptothecin (1:3), artesunate and 7-ethyl camptothecin (1:1), and artesunate and 7-ethyl camptothecin (6:1) had the best inhibitory effects on C. rotundus, which were significantly better than those of artesunate and 7-ethyl camptothecin (1:6) and paeonol and 7-ethyl camptothecin (6:1). The inhibitory effects of the six combinations were significantly better than those of the control herbicide, pretilachlor, and the three plant-derived components. The joint toxicity (Table 2) showed that the six combinations had synergistic effects compared with the single agents.

[0033] The EC50 values for the inhibition of C. rotundus root growth were compared (Table 3). 50 The results (Table 3) showed that the combination of paeonol and 7-ethyl camptothecin (1:3) had the best inhibitory effect, which was significantly better than those of the other combinations and the single agents. The joint toxicity (Table 3) showed that the combination of paeonol and 7-ethyl camptothecin (1:3) had a significant synergistic effect compared with the single agents. The combination of paeonol and 7-ethyl camptothecin (6:1) also had a synergistic effect, and the inhibitory effect on C. rotundus root growth was significantly higher than that of paeonol. In addition, the joint toxicity (Table 3) showed that the four combinations of artesunate and 7-ethyl camptothecin (1:3, 1:6, 3:1 and 6:1) had additive effects.

[0034] Table 2 Dose-response curve parameters of C. rotundus shoot growth to the combinations

[0035]

[0036]

[0037] Table 3 Dose-response curve parameters of C. rotundus root growth to the combinations

[0038]

[0039] 3.2 Inhibition of B. diffusa shoot and root growth by the combination of the three plant-derived components

[0040] Three plant-derived components, paeonol, 7-ethylcamptothecin, and artesunate, showed significantly better inhibitory effects on the broadleaf weed Bidens pilosa than the control herbicide pretilachlor. The combination of plant-derived components exhibited a significant synergistic effect in inhibiting Bidens pilosa.

[0041] By comparing the median concentration (EC) of Bidens pilosa shoot growth inhibitory agent... 50 The results (Table 4) showed that the inhibitory effect of the combination of artesunate and 7-ethylcamptothecin (1:3) was significantly better than that of either agent alone, exhibiting a significant synergistic effect. The two combinations, paeonol and 7-ethylcamptothecin (1:3) and artesunate and 7-ethylcamptothecin (6:1), showed an additive effect.

[0042] By comparing the inhibition of medium concentration (EC) by root length of Bidens pilosa. 50 The results (Table 5) showed that the inhibitory effects of two combinations—artesunate and 7-ethylcamptothecin (1:6) and paeonol and 7-ethylcamptothecin (1:1)—were significantly better than those of the other seven combinations (1:1, 1:3, 6:1) and (6:1, 3:1, 1:3, 1:6). All nine combinations were significantly superior to propachlor. Calculations of the combined toxicity (Table 5) showed that all nine combinations exhibited synergistic effects compared to single-agent formulations.

[0043] Table 4. Parameters of the dose-response curve of Bidens pilosa shoot length to the composition.

[0044]

[0045] Table 5. Parameters of the dose-response curve of Bidens pilosa root length to the composition.

[0046]

[0047]

[0048] 3.3 Inhibitory effect of compound herbicides on shoot and root growth of barnyard grass (Grass family)

[0049] Although the compound composition did not have a significant synergistic effect on barnyard grass, it exhibited excellent inhibitory effects on barnyard grass, comparable to the effects of commonly used pretilachlor.

[0050] By comparing the median concentration (EC) of barnyard grass shoot growth inhibitors 50 The results (Table 6) showed that the two compositions (artesunate and 7-ethylcamptothecin, 1:3) and paeonol and 7-ethylcamptothecin, 1:3, had no significant difference in inhibitory effect on barnyardgrass shoot growth compared to pretilachlor. By calculating the combined toxicity (Table 6), the two compositions had an additive effect compared to single-agent artesunate, 7-ethylcamptothecin, and paeonol. The median concentration (EC50) of barnyardgrass root growth inhibitory agents was compared.50 The results (Table 7) showed that there was no significant difference between the two combinations of Daphneglucon and 7-ethyl camptothecin (1:3) and artesunate and 7-ethyl camptothecin (1:1) and propanil; by calculating the joint toxicity (Table 7), compared with single dose of artesunate, 7-ethyl camptothecin and Daphneglucon, Daphneglucon and 7-ethyl camptothecin (1:3) had additive effect, and artesunate and 7-ethyl camptothecin (1:1) had certain antagonistic effect, but did not significantly reduce the inhibition effect of the composition on the root length of barnyard grass.

[0051] Table 6 Dose response curve parameters of barnyard grass shoot length to the composition

[0052]

[0053]

[0054] Table 7 Dose response curve parameters of barnyard grass root length to the composition

[0055]

[0056] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and not to limit them.

Claims

1. A plant-derived herbicidal composition, characterized by, The active ingredient A is paeonol, the active ingredient B is 7-ethyl camptothecin, and the mass ratio of the active ingredient A to the active ingredient B is 1:6-6:

1.

2. The phytogenic herbicidal composition according to claim 1, characterized in that, The mass ratio of paeonol to 7-ethyl camptothecin is 6:1-1:

3.

3. The phytogenic herbicidal composition according to claim 1 or 2, characterized in that, The weight percentage of the active ingredients is 1-50%.

4. The phytogenic herbicidal composition according to claim 3, characterized in that, The dosage form is wettable powder, microemulsion, aqueous suspension, oil suspension, and emulsifiable concentrate.

5. Use of a phytogenic herbicidal composition according to any one of claims 1 to 4 for controlling weeds in crops, characterized in that, The weeds are grasses, sedges, and broadleaf weeds.

6. Use according to claim 5, characterized in that, The weeds are Echinochloa crus-galli, Cyperus difformis, and Bidens pilosa. The weeds are Echinochloa crus-galli, Cyperus difformis, and Bidens pilosa.

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