A sample analyzer and detection method thereof

Abstract

The present application discloses a sample analyzer and a detection method thereof. The detection includes a control module controlling a reaction module to react a sample and a reagent in a reaction cup to obtain a reaction sample; a control module controlling a first transmission module to transfer a reaction cup containing a reaction sample from the reaction module to a magnetic separation module, and controlling the magnetic separation module to pre-process the reaction sample to obtain a sample to be tested; a control module obtains a detection category in the sample to be tested, and selects one from a chemiluminescence detection module and a flow fluorescence detection module based on the category, for detecting the sample to be tested, and obtaining a detection result. Thus, the chemiluminescence detection module and the flow fluorescence detection module are integrated in the sample analyzer, which can effectively solve the technical problems such as high cost, large space occupation and complex operation caused by the use of the two analyzers separately, and at the same time, compared with the sample analyzer having only one detection module, it can also enrich the detection function of the sample analyzer.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a sample analyzer and a detection method thereof. Background Art

[0002] Immunoassay technology has developed from radioimmunoassay, enzyme-linked immunosorbent assay, plate-based chemiluminescence to magnetic particle chemiluminescence and flow cytometry fluorescence immunoassay. These two immunoassay analysis methods have combined luminescence analysis with immune response to establish microanalysis technology.

[0003] Among them, chemiluminescence immunoassay has the characteristics of high sensitivity and high specificity of antigen-antibody reaction. The instrument detection unit only needs a single photomultiplier tube to receive chemical excitation photon signals. It has a simple structure, short detection time for a single item and low cost. Flow fluorescence integrates fluorescent coded microspheres and specific wavelength laser-excited microspheres to encode luminescence to identify detection items. Another group of specific wavelength laser-excited microsphere antigen or antibody markers emit light for concentration analysis. It uses a flow liquid flow system and high-speed data processing. It has the characteristics of simultaneous joint testing of multiple items on a single sample, parallel detection of multiple items, high throughput, and saving samples and reagents.

[0004] However, in actual usage scenarios, when a sample needs to be tested for multiple items, chemiluminescence can only be used to sample the sample multiple times and test single items in sequence, wasting testing time, reagents and samples. For the elderly or children who have a small amount of blood drawn, it is impossible to test multiple items in one sample. The flow fluorescence immunoassay instrument is relatively expensive and has a complex structure. If a flow immunoassay instrument is used for single-item testing, the test time is long and the cost is high. For some inspection departments or physical examination institutions with many types of test items and many types of single and joint cross-tests, they need to purchase chemiluminescence analyzers and flow fluorescence immunoassay analyzers separately, which wastes costs, takes up a lot of space, and requires more energy to operate different instruments. Summary of the invention

[0005] The main purpose of this application is to provide a sample analyzer and a detection method thereof, aiming to solve the above-mentioned technical problems existing in the prior art.

[0006] To solve the above problems, the present application provides a detection method for a sample analyzer, the sample analyzer comprising a control module, a reaction module, a magnetic separation module, a first transmission module, a chemiluminescence detection module and a flow fluorescence detection module, the reaction module having a reaction cup, the method comprising: the control module controlling the reaction module to react a sample and a reagent in the reaction cup to obtain a reaction sample; the control module controlling the first transmission module to transfer the reaction cup containing the reaction sample from the reaction module to the magnetic separation module, and controlling the magnetic separation module to pre-process the reaction sample to obtain a sample to be tested; the control module obtaining a detection category in the sample to be tested, and selecting one from the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested and obtain a detection result.

[0007] To solve the above problems, the present application provides a sample analyzer, which includes a control module, a reaction module, a magnetic separation module, a first transmission module, a chemiluminescence detection module and a flow fluorescence detection module, wherein the reaction module has a reaction cup; the control module is used to control the reaction module to react the sample and reagent in the reaction cup to obtain a reaction sample; the control module is also used to control the first transmission module to transfer the reaction cup containing the reaction sample from the reaction module to the magnetic separation module, and control the magnetic separation module to pre-process the reaction sample to obtain a sample to be tested; the control module is also used to obtain a detection category in the sample to be tested, and select one from the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested and obtain a detection result.

[0008] Compared with the prior art, the sample analyzer of the present application includes a control module, a reaction module, a magnetic separation module, a first transmission module, a chemiluminescence detection module and a flow fluorescence detection module, wherein the reaction module has a reaction cup, and the detection method includes the control module controlling the reaction module to react the sample and the reagent in the reaction cup to obtain a reaction sample; the control module controls the first transmission module to transfer the reaction cup containing the reaction sample from the reaction module to the magnetic separation module, and controls the magnetic separation module to pre-treat the reaction sample to obtain a sample to be tested; the control module obtains the detection category in the sample to be tested, and selects one from the chemiluminescence detection module and the flow fluorescence detection module based on the category, which is used to detect the sample to be tested and obtain the detection result. Through the above-mentioned implementation, the chemiluminescence detection module and the flow fluorescence detection module are both integrated in the sample analyzer, and one of the two detection modules is selected according to the detection category to detect the sample to be tested, and the detection result is obtained, which can effectively solve the technical problems such as high cost, large space occupation and complex operation caused by the use of two analyzers separately, and at the same time, compared with the sample analyzer having only one detection module, it can also enrich the detection function of the sample analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0010] Figure 1 It is a schematic structural diagram of an embodiment of a sample analyzer provided by the present application;

[0011] Figure 2 is a schematic structural diagram of another embodiment of the sample analyzer provided by the present application;

[0012] Figure 3 It is a schematic flow chart of an embodiment of a detection method of a sample analyzer provided by the present application;

[0013] Figure 4 A schematic diagram of an embodiment of a process for pre-processing a reaction sample provided in the present application;

[0014] Figure 5 It is a schematic diagram of a flow chart of an embodiment of transferring a sample in a reaction cup of a reaction module provided by the present application;

[0015] Figure 6 This is a flow chart of an embodiment of the present application for determining whether a sample needs to be centrifuged;

[0016] Figure 7It is a schematic diagram of a process of separating a tube cap from a tube body according to an embodiment of the present application;

[0017] Figure 8 A schematic diagram of an embodiment of a process for transmitting a sample to a reaction module provided in the present application;

[0018] Fig. 9 A schematic structural diagram of an embodiment of a centrifugal module provided in the present application;

[0019] Fig.10 yes Figure 8 A schematic flow chart of an embodiment of step S802;

[0020] Fig.11 It is a schematic diagram of another embodiment of the flow chart of transferring a sample to a reaction module provided by the present application.

[0021] Figure numbers: sample analyzer 10; control module 100; flow fluorescence detection module 110; chemiluminescence detection module 120; reaction module 200; magnetic separation module 310; mixing module 320; centrifugation module 400; fixing part 410; injection module 510; sample collection tube 511; code scanning module 520; cap removal module 530; tube jacking module 540; scheduling module 600; reagent supply module 700; first transmission module 810; second transmission module 820; third transmission module 830; fourth transmission module 840. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. It will be appreciated that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some but not all structures related to the present application are shown in the drawings. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the art without making creative work are within the scope of protection of the present application.

[0023] The terms "first", "second", "third" in this application are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined as "first", "second", "third" can expressly or implicitly include at least one of the features. In the description of this application, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined. In the embodiments of this application, all directional indications (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication also changes accordingly. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device comprising a series of steps or units is not limited to the steps or units listed, but optionally also includes steps or units that are not listed, or optionally also includes other steps or units inherent to these processes, methods, products or devices.

[0024] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0025] This application provides a sample analyzer, see Figure 1 and Figure 2 , Figure 1 is a schematic structural diagram of an embodiment of a sample analyzer 10 provided by the present application; Figure 2 1 is a schematic diagram of the structure of another embodiment of the sample analyzer 10 provided in the present application.

[0026] The sample analyzer 10 includes a control module 100, a reaction module 200, a magnetic separation module 310, a first transmission module 810, a chemiluminescence detection module 120, and a flow fluorescence detection module 110. The reaction module 200 has a reaction cup. The control module 100 is used to control the above modules in the sample analyzer 10 to perform corresponding functions, and specifically, the sample analyzer 10 can be used to realize the detection function of the sample.

[0027] In order to solve at least one of the above technical problems, the present application also provides a detection method of a sample analyzer 10. Figure 1-Figure 3 , Figure 3 It is a flowchart of an embodiment of a detection method of the sample analyzer 10 provided in the present application.

[0028] The detection method of this embodiment is applied to the sample analyzer 10 described above, and the detection method may include the following steps S301 to S303.

[0029] Step S301: the control module 100 controls the reaction module 200 to react the sample and reagent in the reaction cup to obtain a reaction sample.

[0030] The sample may include a serum sample or a whole blood sample, and the reagent may be selected according to the items to be tested. The sample and the reagent may be directly added to the reaction cup of the reaction module 200, and then the reaction module 200 is used to mix the sample and the reagent in the reaction cup; the sample and the reagent may also be first stored in a reaction cup outside the reaction module 200, and then after being mixed, the reaction cup containing the mixed sample and reagent is directly placed in the corresponding position of the reaction module 200.

[0031] The reaction module 200 can provide an incubation environment for the reaction of samples and reagents. For example, the reaction disk can include an insulation pot and a turntable, wherein the insulation pot is fixed and its main function is to provide a closed space for incubation; the turntable is provided with a certain number of holes for placing reaction cups along the circumferential direction, and is driven to rotate by a motor to facilitate the rotation of the current reaction cup to the required working position; specifically, the required working positions include reagent adding position, sample adding position and stirring position. In addition, a temperature control device is provided at the bottom of the turntable, which can adjust the temperature of the insulation pot to increase the appropriate incubation temperature. In one embodiment, the incubation temperature is 37°C, that is, the incubation temperature can be controlled within the human body temperature range, which is conducive to better reaction of samples and reagents.

[0032] The reaction sample obtained after the reagent and the sample react contains a magnetic bead composition. Specifically, different components in the sample can be measured as needed, and the corresponding antibodies / antigens can be coated on magnetic beads to form magnetic bead reagents, and specific markers can be labeled on antibodies to form labeled reagents (reagents for measuring a certain analysis item generally have multiple components, such as the magnetic bead reagent component and the labeled reagent component here, and different components of the same item can be packaged in different reagent containers or in different cavities of the same reagent container). The sample containing the object to be tested is mixed with the magnetic bead reagent, the labeled reagent and other reagents in turn, and then incubated in the reaction module 200 to form a reaction sample.

[0033] Step S302: the control module 100 controls the first transmission module 810 to transmit the reaction cup containing the reaction sample from the reaction module 200 to the magnetic separation module 310, and controls the magnetic separation module 310 to pre-process the reaction sample to obtain a sample to be tested.

[0034] The first transmission module 810 can directly transfer the reaction cup containing the reaction sample to the magnetic separation module 310. Compared with using a sample suction needle to suck the reaction sample into the reaction cup of the magnetic separation module 310, this solution can allow the magnetic separation module 310 and the reaction module 200 to share the reaction cup, which can simplify material loss.

[0035] The reaction sample is pre-treated by the magnetic separation module 310, that is, the reaction sample is subjected to magnetic separation treatment, and finally the sample to be tested is obtained, that is, the sample to be tested can be extracted from the reaction sample by the magnetic separation module 310. Specifically, the magnetic separation module 310 may include a magnetic separation disk, a liquid injection mechanism and a liquid suction mechanism, the magnetic separation disk is used to absorb the sample to be tested to the side wall of the reaction cup; the liquid suction mechanism is used to absorb the waste liquid in the reaction sample; the liquid injection mechanism is used to inject cleaning liquid into the reaction sample after absorbing the waste liquid to clean the sample to be tested. Specifically, the number of times the cleaning liquid is injected can be determined according to the properties of the added reagents, and the reagents and other wastes in the reaction cup that have not been combined with the magnetic beads can be cleaned. Specifically, the number of cleanings can be 2-6 times, etc.

[0036] Step S303: the control module 100 obtains the detection category of the sample to be tested, and selects one from the chemiluminescence detection module 120 and the flow fluorescence detection module 110 based on the category to detect the sample to be tested and obtain the detection result.

[0037] The flow fluorescence detection module 110 generally includes a flow chamber and an optical detection mechanism; the sample to be tested forms a sheath flow in the flow chamber and passes through the outlet of the flow chamber one by one; the optical detection mechanism is used to detect the optical signal of the sample to be tested passing through the outlet of the flow chamber. Specifically, a laser emitting device is arranged at a position perpendicular to the sheath flow at the outlet of the flow chamber, and a detector is arranged at a position perpendicular to the laser emitting device. The sheath flow, the laser emitter, and the detector are perpendicular to each other and focused on one point to realize fluid dynamic focusing. The sample to be tested, which is fluorescently labeled, emits scattered light and fluorescence emission waves under laser excitation. The scattered light and fluorescence are captured by the detector, and then the optical signal is obtained by a series of filters and gratings to remove interference, and then input into the data analysis device for analysis after photoelectric conversion and amplification.

[0038] The chemiluminescence detection module 120 mainly detects the luminescence intensity of the sample to be tested and receives the chemical excitation photon signal in the sample to be tested to measure the composition and content of the substances contained in the sample.

[0039] The reagent category in the sample to be tested can be determined according to the actual detection category, and the detection category can be determined by the type of reagent, or by scanning related instruments (such as sample racks, sample tube labels, etc.) to identify the detection category. When it is necessary to use multiple projects for parallel detection, the flow fluorescence detection module 110 can be selected. For example, when it is necessary to detect hepatitis B virus, it may be necessary to perform parallel detection on five antigens at the same time. At this time, the reagent category for detecting hepatitis B antigen can be used, and the flow fluorescence detection module 110 can be selected for detection. It can detect multiple projects in parallel at the same time, with high throughput, saving samples and reagents. When only a single project is required for detection, the chemiluminescence detection module 120 can be selected for detection, which has a short detection time and low cost.

[0040] Through the above implementation, the chemiluminescence detection module 120 and the flow fluorescence detection module 110 are integrated into the sample analyzer 10, and the samples to be tested are detected in the two detection modules according to the detection type to obtain the detection results. This can effectively solve the technical problems such as high cost, large space occupation and complicated operation caused by using the two analyzers separately. At the same time, compared with the sample analyzer 10 having only one detection module, it can also enrich the detection function of the sample analyzer 10.

[0041] See also Figure 1-Figure 4 , Figure 4 The present application provides a flowchart of an embodiment of preprocessing a reaction sample. Specifically, controlling the magnetic separation module 310 to preprocess the reaction sample to obtain a sample to be tested may include the following steps S401 to S404.

[0042] Step S401: the control module 100 controls the magnetic separation module 310 to pre-treat the reaction sample to obtain a magnetic bead composition.

[0043] The reaction sample is pre-processed by the magnetic separation module 310, that is, the reaction sample is subjected to magnetic separation to obtain a magnetic bead composition. The magnetic bead composition is a composition mixed with magnetic beads, markers, and antigens adsorbed by the magnetic beads.

[0044] Step S402: Determine whether the number of preprocessing times meets the preset number of repetitions.

[0045] The number of repetitions can be two, three or other times. The number of repetitions is mainly determined by the structure of the sample to be tested. For example, when the sample to be tested is a sandwich structure of antigen + specific antibody + magnetic beads + antibody + fluorescent biotin, after the magnetic beads are combined with the antigen, antibodies and fluorescent biotin need to be added. In the process of preparing the sample to be tested, three incubations and magnetic separation operations are required.

[0046] Step S403: If not, control the first transmission module 810 to transmit the reaction cup containing the magnetic bead composition to the reaction module 200, and use the magnetic bead composition as a sample, return to the step control module 100 to control the reaction module 200 to react the sample and reagent in the reaction cup to obtain a reaction sample.

[0047] When the number of pretreatments does not meet the preset number of repetitions, the first transfer module 810 is controlled to transfer the reaction cup containing the magnetic bead composition to the reaction module 200, and reagents are added thereto, and the process returns to step S301, so that the sample reacts with the magnetic bead composition to obtain a reaction sample.

[0048] Step S404: If yes, the magnetic bead composition is used as a sample to be tested.

[0049] When the number of pretreatments meets the preset number of repetitions, the magnetic bead composition finally obtained is used as a sample to be tested, and one of the chemiluminescence detection module 120 and the flow fluorescence detection module 110 is selected to detect the sample to be tested.

[0050] See also Figure 1-Figure 4 In one embodiment, the sample analyzer 10 includes a mixing module 320. After the magnetic bead composition is used as a sample to be tested (step S404), the detection method further includes: the control module 100 controls the first transmission module 810 to transfer the reaction cup containing the sample to be tested to the mixing module 320; the control module 100 controls the mixing module 320 to mix the sample to be tested.

[0051] The mixing module 320 can be a contact type rotary stirring or ultrasonic vibration stirring, or a non-contact type eccentric vortex mixing or non-contact ultrasonic mixing, etc. The first transmission module 810 can directly transmit the reaction cup containing the sample to be tested to the mixing module 320. Compared with using a sample suction needle to absorb the reaction sample into the reaction cup of the mixing module 320, this solution can make the mixing module 320, the magnetic separation module 310 and the reaction module 200 share the reaction cup in the same sample, which can simplify the loss of materials. The control module 100 controls the mixing module 320 to mix the sample to be tested, and can break up the sample to be tested again so that the sample to be tested can be detected uniformly and orderly.

[0052] After the mixing module 320 is used to mix the sample to be tested, the control module 100 can obtain the detection category in the sample to be tested, and select one from the luminescence detection module and the flow fluorescence detection module based on the detection category to detect the mixed sample to be tested, so as to obtain the detection result.

[0053] See also Figure 1-Figure 5 , Figure 5It is a schematic diagram of a flow chart of an embodiment of transferring a sample in a reaction cup of a reaction module 200 provided in the present application.

[0054] The sample analyzer 10 includes a centrifugal module 400, a sample injection module 510, a second transmission module 820 and a third transmission module 830. Before the control module 100 controls the reaction module 200 to react the sample and reagent in the reaction cup to obtain a reaction sample (step S301), the detection method includes the following steps S501 to S502.

[0055] Step S501 : the control module 100 controls the second transmission module 820 to collect samples from the injection module 510 , and transmits the samples to the centrifugal module 400 , so that the centrifugal module 400 performs centrifugal treatment on the samples.

[0056] The sample injection module 510 stores a sample, which may be a whole blood sample, which has not been centrifuged. The second transmission module 820 contains a sample needle, and the control module 100 controls the sample needle of the second transmission module 820 to extend into and absorb the sample in the injection module 510. The control module 100 controls the second transmission module 820 to move to the centrifugal module 400, and injects the absorbed sample into the centrifugal module 400, so that the sample is centrifuged by the centrifugal module 400 to obtain centrifuged serum and plasma.

[0057] Step S502 : the control module 100 controls the third transfer module 830 to transfer the centrifuged sample to the reaction cup of the reaction module 200 .

[0058] The third transmission module 830 may include a sample needle, and the sample after centrifugation is sucked in through the sample needle, and the sucked sample is injected into the reaction cup of the reaction module 200. The centrifugal module 400 may also directly transfer the reaction cup containing the sample in the centrifugal module 400 to the reaction module 200, so that the centrifugal module 400 and the reaction module 200 share the same reaction cup to accommodate the same sample, thereby reducing the loss of materials.

[0059] The existing sample analyzer 10 usually requires pre-processing of the sample, and the sample is manually placed in a centrifuge for centrifugation. After the whole blood sample is processed into serum or plasma, the processed sample is manually transferred to the sample analyzer 10 for analysis. This operation process consumes a lot of manpower and is time-consuming, which affects the efficiency of the detection work, especially when a small amount of samples needs to be accumulated to a certain amount before detection, which increases the waiting time of the test subject. In addition, the centrifuge is equipped separately, which makes the existing sample analyzer 10 costly and occupies a large space.

[0060] In this solution, the centrifugal module 400 is integrated into the sample analyzer 10 to further realize full automation of sample detection, while also being able to reduce the time for manual sample processing, reduce the manual processing flow, and improve work efficiency.

[0061] See also Figure 1-Figure 6 , Figure 6 It is a flow chart of an embodiment of the present application for determining whether a sample needs to be centrifuged.

[0062] The sample analyzer 10 includes a code scanning module 520, and the sample injection module 510 includes a sample collection tube 511 storing a sample. Before the control module 100 controls the second transmission module 820 to collect a sample from the sample injection module 510 (step S501), the detection method includes the following steps S601 to S604.

[0063] Step S601: The control module 100 controls the code scanning module 520 to identify the identification code on the sample collection tube 511 and obtain the sample information from the identification code.

[0064] The sample collection tube 511 is used to hold the sample. The sample collection tube 511 can be located in the sample rack of the sample injection module 510. The outer surface of the sample collection tube 511 can be affixed with an identification code. For example, the identification code can be a barcode or a QR code. The identification code stores the sample information, which includes the name of the sample collector, the type of sample (serum / plasma after centrifugation, or a whole blood sample), the items to be tested, and the like.

[0065] Step S602: The control module 100 determines whether the sample needs to be centrifuged based on the sample information.

[0066] The sample information includes a sample type. When the sample is serum / plasma after centrifugation, it is determined that the sample does not need to be centrifuged; when the sample is a whole blood sample before centrifugation, it is determined that the sample needs to be centrifuged.

[0067] Step S603 : If yes, the control module 100 controls the second transmission module 820 to collect samples from the injection module 510 and transmit the samples to the centrifugal module 400 , so as to centrifuge the samples.

[0068] If it is determined that the sample needs to be centrifuged, the sample is collected into the centrifugal module 400 in the sample analyzer 10 so as to be centrifuged by the centrifugal module 400 .

[0069] Step S604 : If not, the sample is transferred to the reaction cup of the reaction module 200 .

[0070] If it is determined that the sample does not need to be centrifuged, the sample is directly transferred to the reaction module 200 .

[0071] See also Figure 1-Figure 7 , Figure 7 It is a schematic diagram of a flow chart of an embodiment of separating a tube cap and a tube body provided in the present application.

[0072] The sample analyzer 10 includes a tube jacking module 540 and a cap removal module 530. The sample injection module 510 includes a sample collection tube 511 storing a sample. The sample collection tube 511 includes a tube body and a tube cap covering a port of the tube body. Before the step control module 100 controls the second transmission module 820 to collect a sample from the sample injection module 510 (step S501), the detection method includes:

[0073] Step S701: the control module 100 controls the pipe jacking module 540 to fix the pipe body.

[0074] The sample injection module 510 may also include a test tube rack, which is used to fix the sample collection tube 511. The control module 100 controls the top tube module 540 to abut against the outer wall of the sample collection tube 511 to cooperate with the test tube rack to fix the tube body of the sample collection tube 511. The test tube rack may include a plurality of placement positions for the sample collection tube 511, each placement position may store a sample collection tube 511, and the test tube rack may also move the sample collection tube 511 so that the sample collection tube 511 is located at the top tube module 540.

[0075] Step S702: the control module 100 controls the cap removal module 530 to remove the tube cap from the tube body.

[0076] The diameter of the tube cap is larger than the diameter of the tube body. The sample collection tube 511 can be located between the top tube module 540 and the cap removal module 530. After the sample collection tube 511 is fixed by the top tube module 540, the cap removal module 530 abuts against the outer periphery of the tube cap to separate the tube cap from the tube body. In other embodiments, the tube cap can also be grabbed by the cap removal module 530 and then separated from the tube body. Through the above-mentioned implementation, compared with the method of directly puncturing the tube cap with a sample needle to absorb the sample, this solution can complete automatic cap removal in the sample analyzer 10 and absorb the sample from the sample collection tube 511 after the cap is removed, which can reduce the contamination of the sample caused by the tube cap.

[0077] See also Figure 1-Figure 9 , Figure 8 A schematic diagram of an embodiment of a process for transmitting a sample to a reaction module 200 provided in the present application; Fig. 9 This is a schematic structural diagram of an embodiment of the centrifugal module 400 provided in the present application.

[0078] In one embodiment, the sample analyzer 10 includes a scheduling module 600, and the centrifugal module 400 has a fixing portion 410 and a motor, the fixing portion 410 is used to fix the reaction cup, and the motor is used to drive the fixing portion 410 to rotate to centrifuge the sample in the reaction cup. Among them, the control module 100 controls the third transmission module 830 to transfer the centrifuged sample to the reaction cup of the reaction module 200 (step S502), which may include the following steps S801 to S802.

[0079] Step S801 : the control module 100 controls the third transmission module 830 to transmit the cuvette containing the centrifuged sample to the scheduling module 600 .

[0080] The scheduling module 600 may have a plurality of receiving slots for placing reaction cups to serve as an intermediate transition module for sample reaction, so that samples centrifuged from the centrifugal module 400 can be pre-placed in the scheduling module 600 for temporary storage, thereby avoiding the situation of untimely sample supply and improving the overall detection efficiency of the sample analyzer 10.

[0081] Step S802 : the control module 100 controls the third transmission module 830 to transmit the reaction cup containing the centrifuged sample in the scheduling module 600 to the reaction module 200 .

[0082] In this embodiment, during the entire detection process, the scheduling module 600, the centrifugal module 400, the reaction module 200, the magnetic separation module 310 and the mixing module 320 can share the same reaction cup, which can simplify the loss of materials.

[0083] See also Figure 1-Figure 10 , Fig.10 yes Figure 8 FIG. 8 is a flow chart of an embodiment of step S802 in FIG.

[0084] The sample analyzer 10 further includes a reagent supply module 700 and a fourth conveyor module. The reagent supply module 700 stores reagents for the fourth conveyor module 840 to absorb the reagents. The step (step S802) in which the control module 100 controls the third conveyor module 830 to transfer the reaction cup containing the centrifuged sample in the scheduling module 600 to the reaction module 200 may include the following steps S1001 to S1002.

[0085] Step S1001: the control module 100 controls the fourth transmission module 840 to collect reagents from the reagent supply module 700, and injects the reagents into the reaction cup containing the sample in the scheduling module 600 to obtain a mixture of the sample and the reagent.

[0086] The fourth transmission module 840 has a sampling needle, through which the reagent is sucked from the reagent supply module 700 and the sucked reagent is injected into the reaction cup containing the corresponding sample in the scheduling module 600 .

[0087] The sample and reagent in the same reaction cup are mixed by the scheduling module 600 to obtain a mixture of the sample and the reagent. The sample and reagent in the reaction cup are mixed by the scheduling module 600, so that the sample and the reagent entering the reaction module 200 can react directly. Compared with mixing the sample and the reagent in the reaction cup through the reaction module 200, this solution can improve the overall detection efficiency.

[0088] Step S1002 : the control module 100 controls the third transmission module 830 to transmit the reaction cup containing the mixture in the scheduling module 600 to the reaction module 200 .

[0089] The reaction cup containing the mixture is transferred to the reaction module 200 , and then the reaction module 200 accelerates the reaction of the mixture in the reaction cup.

[0090] See also Figure 1-Figure 11 , Fig.11 It is a schematic diagram of another embodiment of the flow chart of transferring a sample to the reaction module 200 provided in the present application.

[0091] The scheduling module 600 has a plurality of accommodating slots, each of which can be used to accommodate a reaction cup, and the centrifugal module 400 has a plurality of fixing parts 410 for clamping the reaction cup. The step (step S502) in which the control module 100 controls the third transmission module 830 to transfer the centrifuged sample to the reaction cup of the reaction module 200 may include the following steps S1101 to S1102.

[0092] Step S1101 : the control module 100 controls the third transfer module 830 to transfer the cuvettes containing the centrifuged samples from the plurality of fixing parts 410 to the plurality of receiving tanks one by one.

[0093] The number of the fixing parts 410 can be two, four or eight, etc., and the specific number can be determined according to the test items. Each fixing part 410 can fix a reaction cup. When the centrifugal module 400 has multiple fixing parts 410, the centrifugal module 400 can centrifuge samples in multiple reaction cups at the same time. Multiple fixing parts 410 can be controlled to rotate by a driving motor. A centrifugal module 400 can centrifuge samples in multiple reaction cups at the same time, which can improve the efficiency of centrifugation, thereby improving the detection efficiency of the sample analyzer 10. The control module 100 can control the third transmission module 830 to transfer the reaction cups clamped by the multiple fixing parts 410 to the multiple containing tanks one by one.

[0094] Step S1102 : the control module 100 controls the third transfer module 830 to transfer the cuvettes containing the centrifuged samples in the plurality of receiving tanks to the reaction module 200 one by one.

[0095] Through the above-mentioned implementation mode, this solution integrates both the chemiluminescence detection module 120 and the flow fluorescence detection module 110 in the sample analyzer 10, and detects the sample to be tested in the two detection modules according to the detection category to obtain the detection result, which can effectively solve the technical problems such as high cost, large space occupation and complicated operation caused by using the two analyzers separately. At the same time, compared with the sample analyzer 10 having only one detection module, it can also enrich the detection function of the sample analyzer 10.

[0096] Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A detection method of a sample analyzer, It is characterized in that The sample analyzer comprises a control module, a reaction module, a magnetic separation module, a first transmission module, a mixing module, a chemiluminescence detection module and a flow fluorescence detection module. The reaction module has a reaction cup. The method comprises: The control module controls the reaction module to react the sample and reagent in the reaction cup to obtain a reaction sample; The control module controls the first transmission module to transmit the reaction cup containing the reaction sample from the reaction module to the magnetic separation module, and the control module controls the magnetic separation module to pre-treat the reaction sample to obtain a magnetic bead composition; Using the magnetic bead composition as a sample to be tested; The control module controls the first transmission module to transmit the reaction cup containing the sample to be tested to the mixing module; The control module controls the mixing module to perform mixing processing on the sample to be tested; The control module obtains the detection category in the sample to be tested, and selects one from the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested after the mixing process to obtain the detection result; wherein, the control module selects one from the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested after the mixing process to obtain the detection result, including: In response to the detection category including parallel detection of multiple items, selecting the flow fluorescence detection module to detect the sample to be tested after the mixing process to obtain a detection result; In response to the detection category including single item detection, the chemiluminescence detection module is selected to detect the sample to be tested after the mixing process to obtain a detection result.

2. The method according to claim 1, It is characterized in that The step of using the magnetic bead composition as the sample to be tested comprises: Determining whether the number of preprocessing times meets a preset number of repetitions; If not, the first transmission module is controlled to transmit the reaction cup containing the magnetic bead composition to the reaction module, and the magnetic bead composition is used as a sample, and the control module returns to step 1 to control the reaction module to react the sample and reagent in the reaction cup to obtain a reaction sample; If yes, the magnetic bead composition is used as the sample to be tested.

3. The method according to claim 1, It is characterized in that The sample analyzer includes a centrifugal module, a sample injection module, a second transmission module and a third transmission module. The control module controls the reaction module to react the sample and the reagent in the reaction cup. Before obtaining the reaction sample, the method includes: The control module controls the second transmission module to collect the sample from the injection module and transmit the sample to the centrifugal module so as to centrifuge the sample; The control module controls the third transmission module to transmit the centrifuged sample to the reaction cup of the reaction module.

4. The method according to claim 3, It is characterized in that The sample analyzer includes a code scanning module, the sampling module includes a sample collection tube storing the sample, and before the control module controls the second transmission module to collect the sample from the sampling module, the method includes: The control module controls the code scanning module to identify the identification code on the sample collection tube and obtain sample information from the identification code; The control module determines whether the sample needs to be centrifuged based on the sample information; If yes, the step of executing: the control module controls the second transmission module to collect the sample from the injection module and transmit the sample to the centrifugal module so as to centrifuge the sample; If not, the sample is transferred to the reaction cup of the reaction module.

5. The method according to claim 3, It is characterized in that The sample analyzer includes a scheduling module, and the control module controls the third transmission module to transmit the centrifuged sample to the reaction cup of the reaction module, including: The control module controls the third transmission module to transmit the reaction cup containing the centrifuged sample to the scheduling module; The control module controls the third transmission module to transmit the reaction cup containing the centrifuged sample in the scheduling module to the reaction module.

6. The method according to claim 5, It is characterized in that The sample analyzer includes a reagent supply module and a fourth transmission module, and the control module controls the third transmission module to transmit the reaction cup containing the centrifuged sample in the scheduling module to the reaction module, including: The control module controls the fourth transmission module to collect the reagent from the reagent supply module, and inject the reagent into the reaction cup of the scheduling module containing the sample, so as to obtain a mixture of the sample and the reagent; The control module controls the third transmission module to transmit the reaction cup containing the mixture in the scheduling module to the reaction module.

7. The method according to claim 5, It is characterized in that The scheduling module has a plurality of accommodating grooves for accommodating reaction cups, and the centrifugal module has a plurality of fixing parts for clamping the reaction cups; The control module controls the third transmission module to transmit the centrifuged sample to the reaction cup of the reaction module, including: The control module controls the third transmission module to transmit the reaction cups containing the centrifuged samples from the plurality of fixing parts to the plurality of containing tanks one by one; The control module controls the third transmission module to transmit the reaction cups containing the centrifuged samples in the plurality of containing tanks to the reaction module one by one.

8. The detection method according to claim 3, It is characterized in that The sample analyzer further comprises a tube jacking module and a cap removal module, the sample injection module comprises a sample collection tube storing the sample, the sample collection tube comprises a tube body and a tube cap covering a port of the tube body, and before the control module controls the second transmission module to collect the sample from the sample injection module, the method comprises: The control module controls the pipe jacking module to fix the pipe body; The control module controls the cap-removing module to remove the tube cap from the tube body.

9. A sample analyzer, It is characterized in that The sample analyzer comprises a control module, a reaction module, a magnetic separation module, a first transmission module, a mixing module, a chemiluminescence detection module and a flow fluorescence detection module, and the reaction module has a reaction cup; The control module is used to control the reaction module to react the sample and reagent in the reaction cup to obtain a reaction sample; The control module is also used to control the first transmission module to transfer the reaction cup containing the reaction sample from the reaction module to the magnetic separation module, and the control module controls the magnetic separation module to pre-treat the reaction sample to obtain a magnetic bead composition; and use the magnetic bead composition as a sample to be tested; The control module controls the first transmission module to transmit the reaction cup containing the sample to be tested to the mixing module; the control module controls the mixing module to mix the sample to be tested; The control module is further used to obtain the detection category of the sample to be tested, and select one from the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested after the mixing process to obtain the detection result; Wherein, selecting one of the chemiluminescence detection module and the flow fluorescence detection module based on the category to detect the sample to be tested after the mixing process to obtain the detection result includes: In response to the detection category including parallel detection of multiple items, selecting the flow fluorescence detection module to detect the sample to be tested after the mixing process to obtain a detection result; In response to the detection category including single item detection, the chemiluminescence detection module is selected to detect the sample to be tested after the mixing process to obtain a detection result.

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