Method for evaluating the neurotoxic effect of heat stress on eriocheir sinensis and application thereof

By employing a multidimensional sensitive parameter evaluation method, combined with pathological phenotypes, apoptosis gene expression, and neuronal cell damage biomarkers, this study fills the gap in the evaluation of neurotoxicity in Chinese mitten crabs, provides a scientific and reliable detection method, and screens out crab seedlings with strong high-temperature adaptability, supporting the breeding of new high-temperature resistant varieties.

CN117678547BActive Publication Date: 2025-11-21NANJING NORMAL UNIVERSITY
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Patent Information

Application Number
CN202311731283.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-12-14
Publication Date
2025-11-21
Estimated Expiration
2043-12-14

AI Technical Summary

Technical Problem

There is a lack of effective methods in the current technology to evaluate the effects of heat stress on the neurotoxicity of Chinese mitten crab, especially under extreme high temperature conditions, the nervous system is particularly sensitive to heat stress, which affects the assessment of the high temperature tolerance of Chinese mitten crab.

Method used

A multidimensional sensitive parameter evaluation method was adopted, including pathological phenotype, apoptosis gene expression, neuronal cell damage biomarkers, and heat shock response. By setting two temperature groups of 24℃ and 32℃, sampling at different time points, and comprehensively analyzing neurotoxicity, a scientific and reliable evaluation method was provided using indicators such as neuronal apoptosis, DNA damage, and NSE content.

Benefits of technology

This method enables accurate detection and evaluation of neurotoxicity in Chinese mitten crabs, allowing for the screening of crab seedlings with strong high-temperature adaptability. It provides a basis for the breeding of new high-temperature resistant varieties and improves the operability and practicality of the detection.

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Abstract

The application discloses a method for evaluating the influence of heat stress on the neurotoxicity of Eriocheir sinensis and application thereof, wherein the neurotoxicity refers to neuron heat damage toxicity caused by heat stress, the neuron apoptosis, DNA damage, neuron cell damage biomarker (NSE) and heat shock response of Eriocheir sinensis are used as judgment parameters of the neurotoxicity, and the above features are comprehensively analyzed to evaluate the heat damage toxicity of heat stress on the nerve tissue of Eriocheir sinensis. The evaluation method has high reliability, operability and practicability, provides a new method for accurately detecting and evaluating the neurotoxicity of Eriocheir sinensis, and provides help for screening Eriocheir sinensis fry groups with high high-temperature adaptability, high high-temperature tolerance and high survival ability.
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