A dish for rapid separation and counting of copepod eggs

Abstract

The present application provides a kind of dish ware for quickly separating and counting brine shrimp eggs, including dish ware body, the dish ware body includes bottom disc and the sieve tray that can be installed on bottom disc and can rotate in bottom disc, the bottom disc includes disc body, the central position of the disc body is equipped with bearing, the sieve tray includes support, the central position of the support is equipped with mesh screen for separating and counting brine shrimp oocyte, the mesh screen includes four fan-shaped areas of same size;The present application has the following beneficial effects: the present application can separate brine shrimp oocyte from each other and uniformly distribute in culture dish without damaging the appearance structure of brine shrimp oocyte, so that the fertility can be quickly and effectively counted, not only reduces the experimental time cost and labor cost, but also provides a basis for obtaining uniformly distributed brine shrimp oocyte image when measuring egg diameter of subsequent brine shrimp oocyte, so as to ensure the accuracy of egg diameter measurement.

Description

Technical Field

[0001] This invention relates to the field of dish technology, and in particular to a dish for rapidly separating and counting krill eggs. Background Technology

[0002] Research on the reproductive biology of marine organisms often involves fertility statistics and measurement of mature oocyte diameter, which provides a foundation for research on reproductive strategies, resource assessment and conservation management. The fertility statistics of marine organism species require the collection of a large number of experimental samples and the effective separation and counting of oocytes and mature oocytes. This experiment requires a significant investment of time and manpower, especially for experiments on biological samples with high fertility.

[0003] The current experimental procedure is as follows: First, a certain amount of ovarian tissue or mature oocyte sample is cut and placed in a culture dish. Pure water is slowly added, and ovarian oocytes or mature oocytes are carefully separated using a dissecting needle. Then, each ovarian oocyte or mature oocyte is counted under a dissecting microscope. However, in actual fertility counting, the dissecting needle easily damages the oocytes (eggs), causing significant errors in subsequent oocyte diameter measurements. Some oocytes (eggs) are tightly adhered, making forced separation with a dissecting needle difficult, leading to significant errors in fertility counting. The separated oocytes are scattered and disorderly in the culture dish, easily resulting in missed or duplicate counts, causing deviations in fertility counting. Moreover, during the counting process, the movement of the dissecting needle can easily cause water ripples, leading to the scattered movement of oocytes (eggs), greatly increasing the difficulty and time cost of fertility counting. Furthermore, after fertility counting, image acquisition and analysis software (such as Image-Pro) is required. The Plus series of professional image analysis software captures images of evenly distributed oocytes (eggs) for oocyte diameter measurement. However, due to the aforementioned issues, the accuracy of oocyte diameter measurement is reduced. Summary of the Invention

[0004] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide a dish for rapidly separating and counting krill eggs, which solves the problem that the prior art cannot rapidly and effectively separate and count krill oocytes without damaging the external structure of the eggs.

[0005] To achieve the above and other related objectives, the present invention provides the following technical solution:

[0006] A device for rapidly separating and counting krill eggs includes a device body, the device body including a base and a sieve plate that can be mounted on the base and rotated within the base. The base includes a plate body with a bearing at its center. The sieve plate includes a support member, and a mesh screen for separating and counting krill oocytes is installed at the center of the support member. The mesh screen includes four fan-shaped areas of the same size, each fan-shaped area consisting of multiple circular sieve holes, and a shaft is provided at the center of the mesh screen corresponding to the bearing.

[0007] In one embodiment of the present invention, the screen plate and the base plate are nested in an upper and lower structure, and both the screen plate and the base plate are made of PVC material. The PVC used in this technical solution has excellent performance, good corrosion resistance and non-flammability, and also has the advantages of high strength and high stability. It is widely applicable and affordable.

[0008] In one embodiment of the present invention, the bearing is shaped as a cylindrical column, and the shaft includes a shaft body and a shaft base integrally formed with the shaft body. The shaft body is cylindrical, and the shaft base is conical. When the screen plate is installed in the chassis, the shaft base is inserted into the bearing. This technical solution allows the screen plate to rotate within the plate by rotating the shaft within the bearing, which facilitates the entry of krill oocytes into the screen holes of the mesh screen.

[0009] In one embodiment of the present invention, the side wall of the plate is provided with a small beak that facilitates the pouring of krill oocytes out of the plate, and the shape of the small beak is similar to that of a spoon.

[0010] In one embodiment of the present invention, a rim wall is integrally formed with the support member and is used to hold krill oocytes and prevent the krill oocytes from leaking out of the screen. The rim wall is annular in shape and also serves to fix the screen.

[0011] As described above, the dish for rapidly separating and counting krill eggs of the present invention has the following beneficial effects:

[0012] This invention involves pouring krill oocytes to be separated and counted onto a mesh screen. The screen rotates under the action of a shaft and bearings. This rotation allows the krill oocytes to be separated as much as possible and evenly distributed in the culture dish without damaging their external structure. This enables rapid and efficient counting of reproductive capacity, reducing experimental time and labor costs. Furthermore, it provides a basis for obtaining uniformly distributed krill oocyte images during subsequent oocyte diameter measurement, thus ensuring the accuracy of oocyte diameter measurement. Attached Figure Description

[0013] Figure 1 This is a three-dimensional schematic diagram of the dish for rapidly separating and counting krill eggs disclosed in an embodiment of the present invention.

[0014] Figure 2 This is a schematic diagram of the sieve plate in the dish used for rapidly separating and counting krill eggs, as disclosed in an embodiment of the present invention.

[0015] Figure 3 This is a schematic diagram of a mesh screen in a dish used for rapidly separating and counting krill eggs, as disclosed in an embodiment of the present invention.

[0016] Figure 4 This is a schematic diagram of the bearing and shaft in the dish for rapidly separating and counting krill eggs disclosed in an embodiment of the present invention.

[0017] Component designation explanation

[0018] 1. Vessel body; 101. Base plate; 1011. Plate body; 1012. Bearing; 1013. Small beak; 102. Sieve plate; 1021. Support component; 1022. Mesh screen; 1023. Shaft; 1024. Edge wall; 4. Fan-shaped area; 5. Sieve holes. Detailed Implementation

[0019] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. It should be noted that, unless otherwise specified, the following embodiments and features described herein can be combined with each other.

[0020] Please see Figures 1 to 4 This invention provides a dish for rapidly separating and counting krill eggs, comprising a dish body 1, which includes a base plate 101 and a sieve plate 102 that can be mounted on the base plate 101 and rotated within the base plate 101. The sieve plate 102 and the base plate 101 are nested in an upper and lower structure, and both the sieve plate 102 and the base plate 101 are made of PVC material. The PVC used in this invention has excellent performance, good corrosion resistance and non-flammability, and also has the advantages of high strength and high stability, making it widely applicable and affordable.

[0021] exist Figure 1In the process, the base 101 includes a disc body 1011, which is a disc-shaped structure with a diameter of 45 mm, a height of 10 mm, and a thickness of 1 mm. The function of the disc body 1011 is to stabilize the screen 1022 and hold the krill oocytes, so that the oocytes can be filtered and separated quickly and effectively. A bearing 1012 is provided at the center of the disc body 1011. The bearing 1012 is a cylindrical column with a diameter of 6 mm and a height of 5 mm. The bearing 1012 plays an auxiliary role in horizontal stability during the rotation of the screen 102. A small beak 1013 is provided on the side wall of the disc body 1011 to facilitate the pouring of krill oocytes out of the disc body 1011. The small beak 1013 is shaped like a spoon.

[0022] exist Figure 2 and Figure 3 In the sieve disc 102, there is a support member 1021. The support member 1021 is circular in shape. A mesh screen 1022 for separating and counting krill oocytes is installed at the center of the support member 1021. The mesh screen 1022 is a circular mesh screen structure with a side length of 16 mm. The mesh screen 1022 includes four fan-shaped areas 4 of the same size. Each fan-shaped area 4 is composed of multiple circular sieve holes 5. Each fan-shaped area 4 is composed of 100 circular sieve holes 5 with a diameter of 0.6 mm. A shaft 1023 is provided at the center of the mesh screen 1022 corresponding to the bearing 1012. The shaft 1023 includes a shaft body and a shaft bottom integrally formed with the shaft body. The shaft body is cylindrical in shape, and the shaft bottom is conical in shape.

[0023] exist Figure 4 In the process of installing the screen 102 inside the base 101, the bottom of the shaft 1023 is inserted into the bearing 1012. The shaft 1023 is a column with a diameter of 2 mm and a length of 30 mm. The shaft 1023 plays a role in assisting the rotation of the screen 102, thereby facilitating the entry of krill oocytes into the screen holes 5. The support member 1021 is equipped with a circumferential wall 1024, which is integrally formed with the support member 1021 and is used to hold the krill oocytes and prevent the krill oocytes passing through the screen from leaking out. The circumferential wall 1024 is annular in shape, with a radius of 19 mm, a height of 5 mm, and a thickness of 1 mm. The circumferential wall 1024 also plays a role in fixing the screen 1022.

[0024] Specifically, the sieve plate 102 and the base plate 101 form a complete dish 1. Each sieve hole 5 on the mesh 1022 can only filter and hold one oocyte (egg). After filtering and separating the krill oocytes (eggs) using the dish 1, the separated krill oocytes (eggs) are placed into a larger culture dish. Then, the separated oocytes (eggs) are poured into the dish 1. Holding the shaft 1023, the sieve plate 102 is gently rotated to allow each oocyte (egg) to fall into each sieve hole 5, so that 400 oocytes (eggs) can be counted quickly at once. The sieve plate 102 can be removed to easily take out 400 oocytes (eggs). The remaining oocytes (eggs) remain in the base plate 101. Repeating the operation can achieve rapid counting and separation of oocytes (eggs). After counting the oocytes (eggs), they can be placed under a dissecting microscope with a CCD to capture images of the oocytes for egg diameter measurement.

[0025] This invention involves pouring the krill oocytes to be separated and counted onto a mesh screen 1022. The screen 1022 rotates under the action of the shaft 1023 and bearing 1012. This rotation allows the krill oocytes to be separated as much as possible and evenly distributed in the culture dish without damaging their external structure. This enables rapid and effective counting of reproductive capacity, reducing experimental time and labor costs. Furthermore, it provides a basis for obtaining uniformly distributed krill oocyte images during subsequent oocyte diameter measurement, thus ensuring the accuracy of oocyte diameter measurement.

[0026] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. All equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this invention should still be covered by the claims of this invention.

Claims

1. A dish for rapidly separating and counting krill eggs, comprising a dish body (1), characterized in that: The vessel body (1) includes a base plate (101) and a sieve plate (102) that can be mounted on the base plate (101) and rotate within the base plate (101). The sieve plate (102) and the base plate (101) are nested in an upper and lower structure. The chassis (101) includes a disc body (1011), and a bearing (1012) is provided at the center of the disc body (1011). The bearing (1012) is in the shape of a cylindrical column. The sieve disc (102) includes a support member (1021), and a mesh screen (1022) for separating and counting krill oocytes is installed at the center of the support member (1021). The mesh screen (1022) includes four fan-shaped areas (4) of the same size. Each fan-shaped area (4) is composed of multiple circular sieve holes (5). A shaft (1023) is provided at the center of the mesh screen (1022) corresponding to the bearing (1012). The shaft (1023) includes a shaft body and a shaft bottom integrally formed with the shaft body. The shaft body is cylindrical and the shaft bottom is conical. When the sieve disc (102) is installed in the chassis (101), the shaft bottom of the shaft (1023) is inserted into the bearing (1012).

2. The apparatus for rapidly separating and counting krill eggs according to claim 1, characterized in that: Both the sieve plate (102) and the base plate (101) are made of PVC material.

3. The apparatus for rapidly separating and counting krill eggs according to claim 1, characterized in that: The side wall of the disc (1011) is provided with a small beak (1013) to facilitate the pouring of krill oocytes out of the disc (1011), and the small beak (1013) is shaped like a spoon.

4. The apparatus for rapidly separating and counting krill eggs according to claim 1, characterized in that: The support member (1021) is provided with a periphery wall (1024) integrally formed with the support member (1021) and used to hold krill oocytes and prevent the krill oocytes from leaking out after sieving. The periphery wall (1024) is annular in shape.

Images