Method for treating dichlorophenol wastewater by activated sludge

Abstract

The present application relates to a kind of active sludge treatment dichlorophenol wastewater method, it is related to wastewater treatment technical field.The present application includes the following steps: (1) domestication of active sludge: adding bacterial body to active sludge, obtain mixed active sludge;Afterwards, mixed active sludge is added to domestication culture solution, domestication is obtained, and domesticated active sludge is obtained;(2) the treatment of dichlorophenol wastewater: domesticated active sludge is mixed with the dichlorophenol wastewater to be treated, and the treatment of dichlorophenol wastewater is realized;Wherein, the bacterial body used is the mixed bacteria of yellow phanerochaete chrysosporium and corynebacterium glutamicum.The present application can realize the efficient degradation of dichlorophenol, further guarantee the degradation effect of TOC in wastewater, the degradation product is safe, and it has the significant advantage of low energy consumption.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of wastewater treatment, in particular to a method for treating dichlorophenol wastewater by activated sludge. BACKGROUND

[0002] Pharmaceutical and personal care products (PPCPs) are typical representatives of new pollutants, which have been frequently detected in water environment in recent years. Existing studies have shown that many PPCPs have toxicological effects such as hepatorenal toxicity, and may even be carcinogenic, which makes it important and positive to carry out extensive and in-depth PPCPs control and treatment for the safety of water environment.

[0003] Dichlorophenol has a molecular formula of C 13 H 10 Cl2O2, and is a white crystal in appearance, which can be used as a bactericide in agriculture, medicine and soap manufacturing, and also as an anti-worm drug. Dichlorophenol has very high toxicity to aquatic organisms and may have long-term adverse effects on water environment. Therefore, it is an urgent technical problem to be solved to efficiently treat dichlorophenol contaminated wastewater. SUMMARY

[0004] The purpose of the present application is to provide a method for treating dichlorophenol wastewater by activated sludge, so as to solve the problems existing in the prior art and achieve efficient treatment of dichlorophenol contaminated wastewater.

[0005] To achieve the above purpose, the present application provides the following solutions:

[0006] The present application provides a method for treating dichlorophenol wastewater by activated sludge, comprising the following steps:

[0007] (1) Domestication of activated sludge:

[0008] Bacterial bodies are added to the activated sludge to obtain mixed activated sludge; then the mixed activated sludge is added to a domestication culture solution for domestication to obtain domesticated activated sludge;

[0009] (2) Treatment of contaminated water body:

[0010] The domesticated activated sludge is mixed with dichlorophenol wastewater to be treated to achieve treatment of the dichlorophenol wastewater.

[0011] The bacterial bodies are mixed bacteria of Phanerochaete chrysosporium and Corynebacterium glutamicum;

[0012] The solvent of the acclimatization culture solution is water, and the component group per liter is as follows: 0.16-0.18 g of ammonium sulfate, 0.043-0.048 g of potassium dihydrogen phosphate, 0.4-0.8 g of sodium acetate, 0.7-0.9 g of sodium bicarbonate, 0.16-0.18 g of ZnSO4·7H2O, 0.04-0.05 g of Na2MoO4·2H2O, and 0.25-0.35 g of calcium chloride.

[0013] Further, the mass ratio of the Phanerochaete chrysosporium and the Corynebacterium glutamicum is 4-5:1.

[0014] Further, the adding amount of the bacteria is 1.5-5.0% of the mass of the activated sludge.

[0015] Further, the mixed activated sludge is added into the acclimatization culture solution at an adding amount of 25-30 g / L.

[0016] Further, the temperature of the acclimatization is 30-32℃, the dissolved oxygen is 3-3.5 mg / L, the pH value is 7.0-7.2, and the time is 20-23 days. The pH value of the system can be adjusted by sodium hydroxide.

[0017] Further, the volume ratio of the acclimatized activated sludge to the dichlorophenol wastewater to be treated is 1:4.

[0018] Further, the dissolved oxygen of the system in step (2) is 3-3.5 mg / L.

[0019] The application also provides the application of the method for treating dichlorophenol wastewater by using the activated sludge described above in the treatment of dichlorophenol wastewater.

[0020] The application discloses the following technical effects:

[0021] The application uses the activated sludge and adopts the improved biological treatment method to treat the dichlorophenol wastewater, so that the dichlorophenol components in the wastewater can be efficiently removed, the degradation of the dichlorophenol components can be ensured to be efficient and sufficient, the current situation that the dichlorophenol molecules can be damaged to a certain extent but cannot be further degraded more completely can be effectively solved, and a higher TOC degradation rate of the dichlorophenol wastewater can be ensured.

[0022] The application can realize efficient degradation of the dichlorophenol, further ensure the degradation effect of TOC in the wastewater, the degradation product is safe, and the application has the significant advantages of low energy consumption. DETAILED DESCRIPTION

[0023] The following detailed description of various exemplary embodiments of the application should not be considered to limit the application in any way but to describe certain aspects, features, and embodiments of the application.

[0024] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. In addition, where particular ranges of values are given, understand that each intervening value, to the upper or lower limit of the ranges is also specifically included. The upper and lower limits of these smaller ranges can independently be included or excluded in the range, and each smaller range is also encompassed within the application, which contemplates combinations of these smaller ranges within the larger ranges. These are only examples of the various suitable ranges and are not intended to be limiting.

[0025] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All patents, patent applications, publications, and descriptions mentioned herein are incorporated by reference to disclose and describe the methods and / or materials in connection with which the patents, patent applications, publications, and descriptions are cited.

[0026] Many modifications and variations of this application can be made without departing from its spirit or scope, which will be apparent to those skilled in the art. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. The specification and examples given are exemplary only. It is to be understood that the application is not limited in scope by the specific embodiments described herein. Rather, the intent is to embrace all changes and modifications that are within the spirit and scope of the application.

[0027] As used herein, the terms "comprises", "comprising", "includes", "including", "has", "having", "contains", "containing", or variations thereof, are intended to be open-ended terms that mean inclusion, but not limited to, the listed materials and methods.

[0028] The Phanerochaete chrysosporium used in the embodiments of the present application was purchased from China Center for Type Culture Collection, and the preservation number was CCTCC NO: M 2016116; the Corynebacterium glutamicum used in the embodiments of the present application was purchased from China General Microbiological Culture Collection Center, and the preservation number was CGMCC No. 8647.

[0029] The activated sludge used in the embodiments of the present application was taken from a sewage treatment plant; the dichlorophenol simulated wastewater was obtained by dissolving dichlorophenol in water, and the concentration was 100 mg / L. Dichlorophenol (C 13 H 10 Cl2O2, purity ≥95%), purchased from Sigma-Aldrich Co., Ltd., and the structural formula is as follows:

[0030]

[0031] Example 1

[0032] The activated sludge was used to treat the dichlorophenol wastewater, and the steps were as follows:

[0033] (1) Domestication of activated sludge:

[0034] The active sludge is added with 4.5% of the mass of bacteria (mixed bacteria of Phanerochaete chrysosporium and Corynebacterium glutamicum with a mass ratio of 5:1), to obtain mixed active sludge; then the mixed active sludge is added into the acclimation culture solution at an addition amount of 30 g / L, and acclimated at a temperature of 30°C and a dissolved oxygen of 3.3 mg / L for 21 days (the pH value of the system is 7.2); after the acclimation, acclimated active sludge is obtained.

[0035] The solvent of the acclimation culture solution is water, and the composition of each liter is as follows: 0.18 g of ammonium sulfate, 0.048 g of potassium dihydrogen phosphate, 0.5 g of sodium acetate, 0.7 g of sodium bicarbonate, 0.16 g of ZnSO4·7H2O, 0.05 g of Na2MoO4·2H2O, and 0.25 g of calcium chloride.

[0036] (2) Treatment of the contaminated water body:

[0037] The acclimated active sludge obtained in step (1) is mixed with the dichlorophenol simulated wastewater to be treated at a volume ratio of 1:4, and the system is treated at a dissolved oxygen of 3.5 mg / L.

[0038] Example 2

[0039] The dichlorophenol wastewater is treated by using the active sludge, and the steps are as follows:

[0040] (1) Acclimation of the active sludge:

[0041] The active sludge is added with 2.5% of the mass of bacteria (mixed bacteria of Phanerochaete chrysosporium and Corynebacterium glutamicum with a mass ratio of 5:1), to obtain mixed active sludge; then the mixed active sludge is added into the acclimation culture solution at an addition amount of 25 g / L, and acclimated at a temperature of 32°C and a dissolved oxygen of 3.5 mg / L for 21 days (the pH value of the system is 7.1); after the acclimation, acclimated active sludge is obtained.

[0042] The solvent of the acclimation culture solution is water, and the composition of each liter is as follows: 0.17 g of ammonium sulfate, 0.045 g of potassium dihydrogen phosphate, 0.5 g of sodium acetate, 0.8 g of sodium bicarbonate, 0.17 g of ZnSO4·7H2O, 0.05 g of Na2MoO4·2H2O, and 0.3 g of calcium chloride.

[0043] (2) Treatment of the contaminated water body:

[0044] The acclimated active sludge obtained in step (1) is mixed with the dichlorophenol simulated wastewater to be treated at a volume ratio of 1:4, and the system is treated at a dissolved oxygen of 3.5 mg / L.

[0045] Example 3

[0046] The active sludge is used to treat dichlorophenol wastewater, and the steps are as follows:

[0047] (1) Domestication of the active sludge:

[0048] The active sludge is added with 3.5% of the mass of bacteria (mixed bacteria of Phanerochaete chrysosporium and Corynebacterium glutamicum in a mass ratio of 4.5:1), to obtain mixed active sludge; then the mixed active sludge is added into the domestication culture solution in an amount of 28 g / L, and is domesticated at a temperature of 30°C and a dissolved oxygen of 3.2 mg / L for 20 days (the pH value of the system is 7.2); after the domestication, domesticated active sludge is obtained.

[0049] The solvent of the domestication culture solution is water, and each liter of the domestication culture solution contains the following components: 0.16 g of ammonium sulfate, 0.044 g of potassium dihydrogen phosphate, 0.6 g of sodium acetate, 0.8 g of sodium bicarbonate, 0.17 g of ZnSO4·7H2O, 0.03 g of Na2MoO4·2H2O, and 0.30 g of calcium chloride.

[0050] (2) Treatment of the contaminated water body:

[0051] The domesticated active sludge obtained in step (1) is mixed with dichlorophenol simulated wastewater to be treated in a volume ratio of 1:4, and the system has a dissolved oxygen of 3.2 mg / L, to treat the dichlorophenol wastewater.

[0052] Comparative Example 1

[0053] The only difference between Comparative Example 1 and Example 1 is that the Phanerochaete chrysosporium is replaced with an equal amount of Bacillus subtilis.

[0054] Comparative Example 2

[0055] The only difference between Comparative Example 2 and Example 1 is that the Corynebacterium glutamicum is replaced with an equal amount of Phanerochaete chrysosporium.

[0056] Comparative Example 3

[0057] The only difference between Comparative Example 3 and Example 1 is that the components of the domestication culture solution are changed as follows:

[0058] 0.16-0.18 g of ammonium sulfate, 0.043-0.048 g of potassium dihydrogen phosphate, 0.4 g-0.8 g of sodium acetate, 0.7 g-0.9 g of sodium bicarbonate, 0.16-0.18 g of ZnSO4·7H2O, and 2-5 g of sodium chloride.

[0059] The dichlorophenol removal rates and TOC removal rates of the different treatment times of the examples and comparative examples are shown in Table 1.

[0060] Table 1

[0061]

[0062] The destruction and rupture of dichlorophenol molecules can achieve the removal of dichlorophenol, and the green and low-energy biological treatment method is adopted to ensure the high removal rate of dichlorophenol.

[0063] In terms of TOC removal rate, the oxidation of aromatic ring compounds to achieve efficient degradation of dichlorophenol molecules usually requires extremely high energy consumption, such as discharge treatment, and usually cannot achieve complete TOC degradation; the low-energy high biological treatment method is improved, combined with suitable activated sludge domestication method, on the basis of efficient degradation of dichlorophenol, further realizing high TOC removal rate, having the significant advantages of low energy consumption and high treatment efficiency.

[0064] Ames toxicology test of degradation products:

[0065] The salmonella typhimurium test (Ames test) is a short-term biological test method for detecting mutagens, which has the advantages of rapidness, simplicity, sensitivity, etc., and can reflect the anti-mutagenic effect of the test substance at the point mutation level.

[0066] In the Ames test of the present application, TA98 and TA100 strains are selected, and the Ames toxicology test of dichlorophenol degradation products is carried out, and the Ames kit is purchased from Beijing Huizhi Taikang Pharmaceutical Technology Co., Ltd. The plate incorporation method is used in the experiment, and no obvious dose-response relationship is observed in the Ames test, which indicates that the dichlorophenol degradation products have no significant genetic toxicity and potential carcinogenicity.

[0067] The above-described embodiments are only preferred modes of the present application, and do not limit the scope of the present application, and various modifications and improvements of the technical solutions of the present application made by those skilled in the art without departing from the design spirit of the present application shall fall within the protection scope determined by the claims of the present application.

Claims

1. A method for the treatment of dichlorophenol wastewater by activated sludge, characterized by, The method comprises the following steps: (1) domestication of activated sludge: adding bacteria to activated sludge to obtain mixed activated sludge; then adding the mixed activated sludge to a domestication culture solution to obtain domesticated activated sludge; (2) treatment of dichlorophenol wastewater: mixing the domesticated activated sludge with dichlorophenol wastewater to be treated to achieve treatment of the dichlorophenol wastewater; the bacteria are mixed bacteria of Phanerochaete chrysosporium and Corynebacterium glutamicum; the solvent of the domestication culture solution is water, and the composition per liter is: ammonium sulfate 0.16-0.18 g, potassium dihydrogen phosphate 0.043-0.048 g, sodium acetate 0.4-0.8 g, sodium bicarbonate 0.7-0.9 g, ZnSO4·7H2O 0.16-0.18 g, Na2MoO4·2H2O 0.04-0.05 g, and calcium chloride 0.25-0.35 g; the mass ratio of the Phanerochaete chrysosporium and the Corynebacterium glutamicum is 4-5:1; the mixed activated sludge is added to the domestication culture solution at an amount of 25-30 g / L; the domestication temperature is 30-32℃, the dissolved oxygen is 3-3.5 mg / L, the pH value is 7.0-7.2, and the time is 20-23 days; the dissolved oxygen in step (2) is 3-3.5 mg / L, and the treatment time in step (2) is 12-48 h.

2. The method of treating diclosan wastewater with activated sludge according to claim 1, characterized in that, The amount of the bacteria added is 1.5-5.0% of the mass of the activated sludge.

3. The method of treating diclosan wastewater with activated sludge according to claim 1, wherein, The volume ratio of the domesticated activated sludge to the dichlorophenol wastewater to be treated is 1:4.

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