A biological feed for fattening sheep, a preparation method and application thereof

Abstract

The application provides a fattening sheep biological feed and a preparation method and application thereof, and relates to the field of animal feedings.The Saccharomyces cerevisiae provided by the application is specifically Saccharomyces cerevisiae FMSW-1, and the preservation number is CGMCC No.23637.The application also provides application of the Saccharomyces cerevisiae in preparation of the fattening sheep biological feed.The Saccharomyces cerevisiae FMSW-1 can decompose and ferment indigestible macromolecular substances in fermentation materials through metabolism, improve the digestion and absorption utilization rate of the fattening sheep biological feed, and improve the nutritional value of the feed.The reasonable fermentation materials are selected, the Saccharomyces cerevisiae FMSW-1 is compounded with Pediococcus acidilactici for fermentation, the daily weight gain of animals can be improved, the feed intake can be increased, the feed conversion ratio can be reduced, the intestinal flora of animals can be improved, and the immunity of the body can be improved.

Description

[0001] This application is a divisional application of the following application: the application date is January 17, 2022, the application number is 202210050158.4, and the invention name is a saccharomyces cerevisiae, a fattening sheep biological feed, and a preparation method and application thereof. TECHNICAL FIELD

[0002] The present application relates to the field of animal feed, in particular to a fattening sheep biological feed, a preparation method and application thereof. BACKGROUND

[0003] Biological feed refers to a feed product developed by biological engineering technologies such as fermentation engineering, enzyme engineering, protein engineering and genetic engineering, using feed raw materials and additives allowed by relevant national regulations such as "Feed Raw Material Directory (2013)" and "Feed Additive Variety Directory (2013)". The main function of biological feed is to change the composition of gastrointestinal microflora, so that beneficial or harmless microorganisms occupy a dominant population, inhibit the proliferation of pathogenic or harmful microorganisms through competition, and regulate the balance of intestinal microecology. Based on the positive role of biological feed in regulating animal intestinal microbial flora, it has been widely applied in the breeding practice of various types of animals.

[0004] As a green biological feed in the era of non-antibiotic breeding, fattening sheep biological feed has been applied and concerned by the majority of breeders and feed enterprises. Fattening sheep biological feed can improve the fermentation environment of sheep rumen, regulate the balance of digestive tract dominant flora and microorganisms in the body; can improve the digestion and utilization efficiency of feed, improve the production performance and product quality of mutton sheep; can reduce the conversion of protein to ammonia and amine, reduce the odor of feces, and improve environmental sanitation; can also improve the immune function in the intestinal tract of sheep, kill invading bacteria in time, and enhance the immune ability of sheep to diseases.

[0005] The existing technology fattening sheep biological feed is mainly divided into three categories according to different feed additives: fermented feed produced by feed raw materials and microorganisms through fermentation engineering technology, enzymatic feed produced by feed raw materials and enzyme preparations through enzyme engineering technology, and bacteria-enzyme synergistic fermentation feed produced by feed raw materials, enzyme preparations and microorganisms through fermentation engineering and enzyme engineering technology. The fermented feed is prepared by adding microorganisms for fermentation, although the beneficial bacteria in the intestinal tract are introduced, however, since the commonly used feed raw materials for fattening sheep feed are mostly fiber roughage, the fermented feed prepared has high crude fiber content, which affects the digestion and absorption of nutrients in the feed by fattening sheep, and reduces the growth performance and slaughter performance of fattening sheep. The enzymatic feed can enzymatically decompose the indigestible substances in the feed by adding enzyme preparations, promote the digestion and utilization of nutrients in the feed, but it lacks the effect of regulating intestinal microbial flora. The bacteria-enzyme synergistic fermentation feed can take into account the effects of enzymatic decomposition and improvement of intestinal flora, but it needs to add multiple feed additives, which undoubtedly increases the cost of fattening sheep biological feed.

[0006] The present application provides a kind of fermentation bacteria that can improve the nutritional content of fattening sheep biological feed and improve the intestinal flora of animals, which has important practical significance for developing high-quality fermented feed for fattening sheep. SUMMARY

[0007] The purpose of the present application is to provide a new Saccharomyces cerevisiae strain that can improve the nutritional content of fattening sheep biological feed and improve the intestinal flora of animals, thereby improving the production performance of animals and improving the immunity of animals.

[0008] To achieve the above purpose, the present application provides the following technical solutions:

[0009] In the first aspect, the present application provides a Saccharomyces cerevisiae strain FMSW-1, which was deposited with the China General Microbiological Culture Collection Center on October 21, 2021, at the address of No. 1 Beichen West Road, Yard 3, Chaoyang District, Beijing, with the accession number CGMCC No. 23637.

[0010] The Saccharomyces cerevisiae FMSW-1 provided by the present application is derived from a sample collected from wine brewing, and the collection method is as follows: after the mature mountain grape (planted in Shenyang Fengmei Biotechnology Co., Ltd.) is picked, it is put into a sealed tank, 20% of the mass of the mountain grape is added with white sugar, and then the sealed fermentation is carried out at 32 DEG C for 50 days, bubbles are generated in the sealed tank and there is an alcoholic aroma, and then a proper amount of wine liquid is taken out to be selectively cultured in a malt wort agar medium (malt extract powder 130 g / L, chloramphenicol 0.1 g / L, agar 40 g / L, and the rest is water, pH 5.6), and then the culture is carried out at 37 DEG C for 48 h, and then the surface of the culture medium grows the milk-white, round, smooth-surfaced and slightly bulging colonies, and then a single colony with large and full colonies is selected to be subcultured in the malt wort agar medium, and then the subculture is carried out for 3-4 generations to obtain a single strain, i.e., the Saccharomyces cerevisiae FMSW-1, and then the microscope observation is carried out, as shown in Figure 1

[0011] In a second aspect, the present application provides an application of the Saccharomyces cerevisiae in the preparation of biological feed for fattening sheep.

[0012] In a third aspect, the present application provides a biological feed for fattening sheep, which comprises the Saccharomyces cerevisiae.

[0013] Further, the biological feed for fattening sheep comprises fermentation base material, water and a compound bacterial liquid, and the compound bacterial liquid comprises a Saccharomyces cerevisiae bacterial liquid and a pediococcus acidilacticii bacterial liquid, wherein the Saccharomyces cerevisiae bacterial liquid is the bacterial liquid of the Saccharomyces cerevisiae in claim 1.

[0014] Further, in the pediococcus acidilacticii bacterial liquid, the preservation number of the pediococcus acidilacticii is CGMCC No. 20658.

[0015] The pediococcus acidilacticii is derived from a sample collected from a hand-made sauerkraut workshop, and on September 16, 2020, it was preserved in the China General Microbiological Culture Collection Center, and the address of the preservation unit is No. 3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 20658. The strain has been disclosed in a patent document with the application number 202110416893.8 and the invention name "high-digestibility pediococcus acidilacticii fermented wet soybean meal and preparation method thereof".

[0016] Further, according to the weight fraction, the raw materials comprise the following components: fermentation base material 70-80 parts, water 15-25 parts and compound bacterial liquid 5-15 parts,

[0017] Further, according to the weight fraction, the raw materials comprise the following components: fermentation base material 70 parts, water 20 parts and compound bacterial liquid 10 parts.

[0018] ​Further, the raw materials include the following components in parts by weight: fermentation base 70 parts, water 25 parts, and compound bacterial liquid 5 parts.

[0019] Further, the raw materials include the following components in parts by weight: fermentation base 70 parts, water 15 parts, and compound bacterial liquid 15 parts.

[0020] Further, the mass ratio of the Saccharomyces cerevisiae bacterial liquid and the Pediococcus acidilactici bacterial liquid is 5-7:3-5.

[0021] Further, the viable bacterial count of the Saccharomyces cerevisiae bacterial liquid and the Pediococcus acidilactici bacterial liquid is 1.0×10 9 -1.0×10 10 CFU / mL.

[0022] Further, the preparation method of the Saccharomyces cerevisiae bacterial liquid includes: inoculating the Saccharomyces cerevisiae into a wort liquid culture medium at an inoculation amount of 1-10%, culturing at 32-38°C for 24-72h, and activating 2-3 generations.

[0023] Further, the preparation method of the Saccharomyces cerevisiae bacterial liquid includes: inoculating the Saccharomyces cerevisiae into a wort liquid culture medium at an inoculation amount of 1%, culturing at 37°C for 24h, and activating 3 generations.

[0024] Further, the preparation method of the Pediococcus acidilactici bacterial liquid includes: inoculating the Pediococcus acidilactici into a MRS liquid culture medium at an inoculation amount of 1-10%, culturing at 32-38°C for 24-72h, and activating 2-3 generations.

[0025] Further, the preparation method of the Pediococcus acidilactici bacterial liquid includes: inoculating the Pediococcus acidilactici into a MRS liquid culture medium at an inoculation amount of 1%, culturing at 37°C for 24h, and activating 3 generations.

[0026] Further, the wort liquid culture medium is composed of the following components: malt extract powder 130g / L, chloramphenicol 0.1g / L, and the rest is water, with pH value being 5.4-5.8.

[0027] Further, the MRS liquid culture medium is composed of the following components: casein enzymatic digest 10.0g / L, ammonium citrate tribasic 2.0g / L, beef extract powder 10.0g / L, magnesium sulfate 0.2g / L, yeast extract powder 4.0g / L, manganese sulfate 0.05g / L, sodium acetate 5.0g / L, dipotassium hydrogen phosphate 2.0g / L, glucose 20.0g / L, Tween-80 1.08g / L, and the rest is water, with pH value being 5.5-5.9.

[0028] Further, the fermentation base includes pressed corn, bran, and sprayed corn skin at a mass ratio of 3-5:2-4:3-4.

[0029] Further, the fermentation material comprises any one of the following mass ratios of pressed corn, bran and sprayed corn skin: 4:3:3, 3:4:4 and 5:2:3.

[0030] In a fourth aspect, the present application provides a preparation method of the fattening sheep biological feed, comprising the following steps: mixing the fermentation material, water and compound bacterial liquid, and then culturing at 32-38 DEG C for 24-96h.

[0031] Further, the fermentation material, water and compound bacterial liquid are mixed, and then cultured at 37 DEG C for 72h.

[0032] In a fifth aspect, the present application provides application of the fattening sheep biological feed or the fattening sheep biological feed prepared by the preparation method in improving animal production performance and / or improving animal body immunity.

[0033] The technical scheme of the present application has the following advantages:

[0034] The Saccharomyces cerevisiae FMSW-1 provided by the present application can decompose and ferment the indigestible macromolecular substances in the fermentation material through metabolism, improve the digestion and absorption utilization rate of the fattening sheep biological feed, and improve the nutritional value of the feed. Compared with other Saccharomyces cerevisiae, the Saccharomyces cerevisiae FMSW-1 can significantly improve the live bacteria content, crude protein content, acid-soluble protein proportion and total acid content in the feed, and can also significantly reduce the crude fiber content. As for the amino acid content, the fattening sheep biological feed prepared by the Saccharomyces cerevisiae FMSW-1 has higher essential amino acid content and flavor amino acid content than that prepared by other Saccharomyces cerevisiae, wherein the essential amino acid is beneficial to improve the animal production performance, and the increase of the flavor amino acid content is helpful to improve the feed flavor and increase the animal food intake.

[0035] The fattening sheep biological feed provided by the present application can improve the daily weight gain of animals, increase the food intake, reduce the feed conversion ratio, and indirectly improve the economic benefits by selecting reasonable fermentation material and compounding and fermenting the Saccharomyces cerevisiae FMSW-1 and Pediococcus acidilactici. Meanwhile, the fattening sheep biological feed provided by the present application can reduce the number of harmful intestinal bacteria Escherichia coli in feces, increase the number of intestinal probiotic lactic acid bacteria in feces, improve the intestinal flora of animals, and improve the body immunity. BRIEF DESCRIPTION OF DRAWINGS

[0036] In order to more clearly illustrate the technical solutions in the specific embodiments or prior art of the present application, the drawings needed in the description of the specific embodiments or prior art will be briefly introduced. Obviously, the drawings described below are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort on the basis of these drawings.

[0037] Figure 1 is a picture of Saccharomyces cerevisiae FMSW-1 provided by the present application observed under a microscope. DETAILED DESCRIPTION

[0038] The following examples are provided to better further understand the present application and are not limited to the best mode, and do not limit the content and scope of protection of the present application. Any person who obtains any product the same as or similar to the present application under the inspiration of the present application or by combining the present application with other prior art features falls within the scope of protection of the present application.

[0039] The pediococcus acidilacticii used in the embodiments of the present application is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 20658.

[0040] The malt wort liquid medium in the embodiments of the present application is composed of the following components: malt extract powder 130 g / L, chloramphenicol 0.1 g / L, and the rest is water, pH 5.4-5.8.

[0041] The MRS liquid medium in the embodiments of the present application is composed of the following components: casein enzymatic digest 10.0 g / L, ammonium citrate tribasic 2.0 g / L, beef extract powder 10.0 g / L, magnesium sulfate 0.2 g / L, yeast extract powder 4.0 g / L, manganese sulfate 0.05 g / L, sodium acetate 5.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, glucose 20.0 g / L, Tween-80 1.08 g / L, and the rest is water, pH 5.5-5.9.

[0042] If the specific experimental steps or conditions are not specified in the embodiments, the operation or conditions of the conventional experimental steps described in the literature in the art can be used. The raw materials or instruments used are conventional products that can be obtained by purchase, including but not limited to the raw materials or instruments used in the embodiments of the present application.

[0043] Embodiment 1

[0044] The present embodiment provides a fattening sheep biological feed, and the preparation method is as follows:

[0045] (1) Preparation of composite bacteria solution

[0046] Saccharomyces cerevisiae FMSW-1 was inoculated into wort liquid medium at an inoculation amount of 1%, and cultured at 37°C for 24h for activation. The activated Saccharomyces cerevisiae seed solution was inoculated into wort liquid medium at an inoculation amount of 1%, and cultured at 37°C for 24h. The above steps were repeated for 3 generations. The viable cell count was up to 10 9 CFU / mL, and a Saccharomyces cerevisiae bacteria solution was obtained.

[0047] Pediococcus acidilactici was inoculated into MRS liquid medium at an inoculation amount of 1%, and cultured at 37°C for 24h for activation. The activated Pediococcus acidilactici seed solution was inoculated into MRS liquid medium at an inoculation amount of 1%, and cultured at 37°C for 24h. The above steps were repeated for 3 generations. The viable cell count was up to 10 9 CFU / mL, and a Pediococcus acidilactici bacteria solution was obtained.

[0048] The Saccharomyces cerevisiae bacteria solution and the Pediococcus acidilactici bacteria solution were mixed at a mass ratio of 1:1 to obtain a composite bacteria solution.

[0049] (2) Preparation of fermentation material

[0050] The spray corn husk and the bran were crushed, and the flaked corn was not crushed. The flaked corn, the bran, and the spray corn husk were mixed at a mass ratio of 4:3:3 to obtain a fermentation material.

[0051] (3) Fermentation

[0052] The prepared fermentation material and the composite bacteria solution were mixed at a ratio of 70% fermentation material, 20% water, and 10% composite bacteria solution, and then fermented at 37°C for 72h to obtain a biological feed for fattening sheep.

[0053] Example 2

[0054] The biological feed for fattening sheep was prepared according to the method of Example 1, except that in step (3), the fermentation material, water, and the composite bacteria solution were mixed at a ratio of 70%, 25%, and 5%, respectively.

[0055] Example 3

[0056] The biological feed for fattening sheep was prepared according to the method of Example 1, except that in step (3), the fermentation material, water, and the composite bacteria solution were mixed at a ratio of 70%, 5%, and 15%, respectively.

[0057] Example 4

[0058] The biological feed for fattening sheep was prepared according to the method of Example 1, except that in step (2), the flaked corn, the bran, and the spray corn husk were mixed at a mass ratio of 3:4:4.

[0059] Example 5

[0060] This example provides a fattening sheep biological feed, the preparation method thereof is referred to Example 1, the only difference is that in step (2), the mass ratio of pressed corn, bran and sprayed corn bran is 5:2:3.

[0061] Comparative Example 1

[0062] This comparative example provides a fattening sheep biological feed, the preparation method thereof is referred to Example 1, the only difference is that the Angel brewing yeast bacteria liquid is used instead of the brewing yeast bacteria liquid in Example 1.

[0063] The Angel brewing yeast is a high-temperature-resistant brewing high-activity dry yeast, which is purchased from Angel Yeast Co., Ltd. and is a milky white granule. 1 g thereof is dissolved in 100 mL of sterilized water, 100 uL of which is coated on a malt juice agar culture medium (malt extract powder 130 g / L, chloramphenicol 0.1 g / L, agar 40 g / L, and the rest is water, pH value 5.4-5.8) for selective isolation and culture. After being cultured at 37°C for 48 h, milky white, round, smooth-surfaced and slightly bulging colonies are grown on the surface of the culture medium. Then, a single colony with large and full colonies is selected for subculture in the malt juice agar culture medium, and the single strain is obtained after being subcultured for 3-4 generations. The single colony is inoculated into the malt juice liquid culture medium, and is activated at 37°C for 24 h. Then, the activated Angel brewing yeast seed liquid is inoculated into the malt juice liquid culture medium at an inoculation amount of 1%, and is cultured at 37°C for 24 h. The above steps are repeated for 3 generations of activation, and the number of viable bacteria is detected to be 10 9 CFU / mL, and the Angel brewing yeast bacteria liquid is obtained.

[0064] Comparative Example 2

[0065] This comparative example provides a fattening sheep biological feed, the preparation method thereof is referred to Example 1, the only difference is that the Guangzhou Weiyuan brewing yeast bacteria liquid is used instead of the brewing yeast bacteria liquid in Example 1.

[0066] Guangzhou Weiyuan Saccharomyces cerevisiae, purchased from Guangzhou Weiyuan Biotechnology Co., Ltd., yeast powder, feed active dry yeast, milk gray particles. 1 g was dissolved in 100 mL of sterile water, 100 uL was coated on malt juice agar medium (malt extract powder 130 g / L, chloramphenicol 0.1 g / L, agar 40 g / L, the rest is water, pH 5.4-5.8) for selective isolation and culture, and incubated at 37°C for 48 h. The surface of the culture medium grew white, round, smooth surface, slightly raised colonies, then selected single colonies with large and full colonies for subculture in malt juice agar medium, and subcultured for 3-4 generations to obtain a single strain. The single colony was inoculated into the malt juice liquid medium, and incubated at 37°C for 24 h for activation. The activated Saccharomyces cerevisiae seed liquid was inoculated into the malt juice liquid medium at an inoculation amount of 1%, and incubated at 37°C for 24 h. According to the above steps, a total of 3 generations were activated, and the viable cell count was up to 10 9 CFU / mL, and the bacterial liquid of Guangzhou Weiyuan Saccharomyces cerevisiae was obtained.

[0067] Experimental Example 1

[0068] The purpose of this experimental example is to detect the nutritional indicators of the fattening sheep biological feed prepared in Examples 1-5 and Comparative Examples 1-2, including: total viable count, crude protein content, acid-soluble protein content, crude ash content, total acid content, crude fiber content, and content of 17 kinds of amino acids. The detection methods of each index are as follows:

[0069] Detection method of total viable count:

[0070] Take 25 g of sample with sterile operation and place it in a sterile homogenization cup containing 225 mL of physiological saline. Homogenize at 10,000 r / min for 2 min to prepare a 1:10 sample homogenate. Use a 1 mL sterile pipette or micropipette to take 1 mL of the 1:10 sample homogenate and slowly inject it along the wall of a sterile test tube containing 9 mL of physiological saline. Vortex and mix thoroughly to prepare a 1:100 sample homogenate. Take another 1 mL sterile pipette or micropipette tip and follow the above operation sequence to prepare 10-fold incremental sample homogenates. Each time the dilution is increased by 1, a new 1 mL sterile pipette or tip is used. According to the estimated total viable count of the sample to be tested, select 3 consecutive appropriate dilutions. Take 0.1 mL of sample homogenate from each dilution and place it in 2 agar plates. Use an L-shaped rod for surface coating. After 48 h of incubation in a 30±1°C incubator, count all the colonies on the plates. From sample dilution to plate coating, try to complete it within 30 min.

[0071] Result calculation:

[0072]

[0073] The viable count of the product is the total viable count detected.

[0074] The report is issued according to the results of colony counting, the sample is weighed and reported in units of CFU / g, and the volume sample is reported in units of CFU / mL.

[0075] The detection method of crude protein content refers to GB / T 6432-2018 Determination of crude protein in feed - Kjeldahl method;

[0076] The detection method of acid-soluble protein content refers to NY / T 3801-2020 Determination of acid-soluble protein in feed materials - Acid-soluble protein / crude protein;

[0077] The detection method of crude ash content refers to GB / T 6438-2007 Determination of crude ash in feed;

[0078] The detection method of total acid content refers to GB 12456-2021 National food safety standard Determination of total acid in food;

[0079] The detection method of crude fiber content refers to GB / T 6434-2006 Determination of crude fiber content in feed - Filtration method;

[0080] The detection method of amino acid content refers to GB 5009.235-2016 National food safety standard Determination of amino acid nitrogen in food - Colorimetric method.

[0081] As a control, the nutritional indicators of the fermentation substrate prepared in step (2) of Example 1 were also detected according to the above method.

[0082] The detection results of Examples 1, 1-2 are compared in Table 1, the detection results of Examples 1-3 are compared in Table 2, and the detection results of Examples 1, 4-6 are compared in Table 3.

[0083] Table 1 Comparison of detection results of Examples 1, 1-2

[0084]

[0085]

[0086] As shown in Table 1, the experimental results show that the fermentation of the biological feed for fattening sheep using the Saccharomyces cerevisiae FMSW-1 provided by the present application increases the crude protein content by 32.48%, the acid-soluble protein content by 32.9%, the total acid content by 81.4%, and reduces the crude fiber content by 2% compared with the fermentation substrate before fermentation. Compared with the biological feed for fattening sheep fermented by the Saccharomyces cerevisiae of Angel and the Saccharomyces cerevisiae of Guangzhou Weiyuan, the biological feed for fattening sheep fermented by the Saccharomyces cerevisiae FMSW-1 provided by the present application significantly increases the total number of viable bacteria, the crude protein content, the acid-soluble protein content, the total acid content, and the content of each amino acid, and significantly reduces the crude fiber content. Among them, the content of essential amino acids and flavor amino acids in the biological feed for fattening sheep fermented by the Saccharomyces cerevisiae FMSW-1 provided by the present application is increased, the essential amino acids are beneficial to improve the production performance of animals, and the flavor amino acids are beneficial to improve the flavor of the feed, thereby increasing the food intake of animals.

[0087] Table 2 Comparison of detection results of Examples 1-3

[0088]

[0089]

[0090] As shown in Table 2, the experimental results show that the fermentation of different proportions of compound bacteria liquid has an effect on the nutritional indicators of the biological feed for fattening sheep. After 10% compound bacteria liquid treatment, the optimization rate of the nutritional indicators is higher than that of 5% and 15% compound bacteria liquid fermentation. Although some indicators are also good after 15% compound bacteria liquid inoculation fermentation, 10% compound bacteria liquid inoculation is the most suitable considering the cost.

[0091] Table 3 Comparison of detection results of Examples 1, 4-5

[0092]

[0093]

[0094] As shown in Table 3, the experimental results show that there are differences in the nutritional indicators of the biological feed for fattening sheep prepared by fermentation of different substrate ratios of fermentation substrate. When the mass ratio of flaked corn, bran, and sprayed corn bran is 4:3:3, the feed is the most nutritious, can meet the nutritional needs of animals, improve the immunity of the body, has the highest content of amino acid components, and has a high content of flavor amino acids, which is beneficial to improve the palatability of animals.

[0095] Experimental Example 2

[0096] The present experimental example aims to study the effect of the biological feed for fattening sheep provided by the present application on the production performance and intestinal flora of fattening sheep.

[0097] The experiment was carried out in the fattening stage, 150 fattening sheep (Jilin Songyuan City Qianan Road Village) with a body weight of about 30 kg were randomly divided into 3 groups, 5 replicates per group, 10 sheep per replicate, respectively control group, experimental group 1, experimental group 2, each group was fed as follows:

[0098] Control group: feeding fattening sheep diet.

[0099] Experimental group 1: feeding fattening sheep diet + fattening sheep biological feed prepared by example 5, the addition amount of fattening sheep biological feed is 10% of fattening sheep diet.

[0100] Experimental group 2: feeding fattening sheep diet + fattening sheep biological feed prepared by example 5, the addition amount of fattening sheep biological feed is 20% of fattening sheep diet.

[0101] Each day feeding fattening sheep diet + fattening sheep biological feed a total of 3 kg, feeding three times, 1 kg each time, respectively 8:00 in the morning, 12:00 in the afternoon, 5:00 in the evening; After feeding, free feeding for 2-3 hours, then record the intake. The experimental period is 30 days. The composition and nutritional content of fattening sheep diet are shown in Tables 4-5.

[0102] Table 4 Fattening sheep diet composition

[0103] Raw materials Ratio (%) Corn 44 Corn stalk 17.5 DDGS 24 Soybean meal (43) 5 Stone powder 1 Ammonium chloride 0.5 Salt 0.5 Baking soda 0.5 Soybean hull 7 Total 100

[0104] Table 5 Nutritional content of fattening sheep diet

[0105]

[0106]

[0107] The production performance indexes of control group and experimental groups 1-2 were statistically analyzed by SPSS 9.1 (2003) software using one-way ANOVA, the results were expressed as "mean ± standard deviation", the method of multiple comparison was LSD, P<0.01 (extremely significant difference), P<0.05 (significant difference) were used as the judgment standard of significant difference, and the results are shown in Table 6.

[0108] Table 6 Results of fattening sheep production performance indexes of control group and experimental groups 1-2

[0109]

[0110] Note: The same row data with the same lowercase letter indicates no significant difference (P>0.05), the adjacent lowercase letters indicate significant difference (P<0.05), and the inter-letter lowercase letters indicate extremely significant difference (P<0.01).

[0111] As shown in Table 6, the addition of biological feed for fattening sheep can increase the daily weight gain, increase the feed intake, and reduce the feed conversion ratio, among which the feed conversion ratio is reduced the most when the addition amount is 20%, which can indirectly improve the economic benefits.

[0112] After the experiment, the number of E. coli and lactic acid bacteria in the feces of fattening sheep was detected, and the detection method referred to the total number of viable bacteria. The results of each group were expressed in the form of mean within the group, and the results are shown in Table 7.

[0113] Table 7 Fecal microbial detection results of fattening sheep in control group and experimental groups 1-2

[0114] Group Control group Experimental group 1 Experimental group 2 Escherichia coli (cfu / g) 2.13 x 10 7 ]] 5.02 x 10 5 ]] <![CDATA[1.87×10 4 ]]> Lactic acid bacteria (cfu / g) 5.47 x 10 5 ]]> 6.88 x 10 7 ]]> 4.01 x 10 8 ]]

[0115] As shown in Table 7, the addition of biological feed for fattening sheep can reduce the number of harmful intestinal bacteria E. coli in feces and increase the number of intestinal probiotic bacteria lactic acid bacteria in feces. The health of intestinal flora can be indirectly reflected from the fecal microbial situation, which shows that feeding biological feed for fattening sheep can significantly improve the intestinal flora of animals and improve the immunity of the body.

[0116] Obviously, the above examples are only examples for clearly illustrating, but not limiting the embodiments. For those skilled in the art, other different forms of changes or variations can be made on the basis of the above description. Here, all the embodiments need not and cannot be exhausted. The obvious changes or variations derived therefrom are still within the protection scope of the present application.

Claims

1. A biological feed for fattening sheep, characterized in that: The raw materials include the following components in parts by weight: 70-80 parts of fermentation base, 15-25 parts of water and 5-15 parts of compound bacterial liquid. The composite bacterial solution comprises a saccharomyces cerevisiae bacterial solution and a Pediococcus acidilactici bacterial solution, wherein the saccharomyces cerevisiae bacterial solution is a saccharomyces cerevisiae bacterial solution ( Saccharomyces cerevisiae ) FMSW-1 bacterial solution, the deposit number of the cerevisiae yeast is CGMCC No. 23637; The mass ratio of the saccharomyces cerevisiae bacterial solution to the Pediococcus acidilactici bacterial solution is 5-7:3-5; The number of viable bacteria in the Saccharomyces cerevisiae bacterial solution and the Pediococcus acidilactici bacterial solution was 1.0×10 9 ~1.0×10 10 CFU / mL; The fermentation base material comprises flaked corn, bran and sprayed corn husk in a mass ratio of 3-5:2-4:3-4.

2. The biological feed for fattening sheep according to claim 1, characterized in that The raw materials include the following components in parts by weight: 70 parts of fermentation base, 20 parts of water and 10 parts of compound bacterial liquid, or 70 parts of fermentation base, 25 parts of water and 5 parts of compound bacterial liquid, or 70 parts of fermentation base, 15 parts of water and 15 parts of compound bacterial liquid.

3. The biological feed for fattening sheep according to claim 1, characterized in that The Pediococcus acidilactici bacterial liquid has a preservation number of CGMCC No. 20658.

4. The biological feed for fattening sheep according to claim 1, characterized in that The preparation method of the brewer's yeast liquid comprises: inoculating the brewer's yeast into a malt wort liquid culture medium at an inoculum rate of 1-10%, culturing at 32-38° C. for 24-72 hours, and activating for 2-3 generations; The wort liquid culture medium is composed of the following components: 130 g / L malt extract powder, 0.1 g / L chloramphenicol, and the rest is water, with a pH value of 5.4-5.8; The preparation method of the Pediococcus acidilactici bacterial liquid comprises: inoculating the Pediococcus acidilactici into an MRS liquid culture medium at a 1-10% inoculation rate, culturing at 32-38° C. for 24-72 hours, and activating for 2-3 generations; The MRS liquid culture medium is composed of the following components: 10.0 g / L casein enzyme digest, 2.0 g / L ammonium citrate tribasic, 10.0 g / L beef extract powder, 0.2 g / L magnesium sulfate, 4.0 g / L yeast extract powder, 0.05 g / L manganese sulfate, 5.0 g / L sodium acetate, 2.0 g / L potassium hydrogen phosphate, 20.0 g / L glucose, 1.08 g / L Tween-80, and the remainder is water, with a pH value of 5.5-5.

9.

5. The biological feed for fattening sheep according to claim 4, characterized in that: The preparation method of the brewer's yeast liquid comprises: inoculating the brewer's yeast into a malt wort liquid culture medium at a 1% inoculation rate, culturing at 37° C. for 24 hours, and activating for three generations; The preparation method of the Pediococcus acidilactici bacterial liquid comprises: inoculating the Pediococcus acidilactici into an MRS liquid culture medium at a 1% inoculation rate, culturing at 37° C. for 24 hours, and activating for three generations.

6. The biological feed for fattening sheep according to claim 1, characterized in that: The fermentation base material comprises flaked corn, bran and sprayed corn husk in a mass ratio of 4:3:3 or 3:4:4 or 5:2:

3.

7. The method for preparing the biological feed for fattening sheep according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: mixing the fermentation base material, water and composite bacterial liquid and culturing at 32-38° C. for 24-96 hours.

8. The method for preparing the biological feed for fattening sheep according to claim 7, characterized in that: The fermentation base, water and composite bacterial solution were mixed and cultured at 37° C. for 72 hours.

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