A method for grafting tree rose by using intermediate stock

Abstract

The application provides a method for grafting tree-shaped Chinese rose by using an intermediate stock, and the method comprises the following steps: 1) culturing a rose rootstock; 2) selecting a rose branch with a smaller diameter than the rootstock and being compatible with the rootstock and a target scion; 3) immersing the intermediate stock branch and the target scion branch in rooting powder before grafting; 4) immersing the intermediate stock branch in a transpiration inhibitor before grafting; 5) grafting the target scion and the intermediate stock by using the methods of bud grafting, wedge grafting or cutting grafting (insertion grafting-belly grafting); and 6) reinforcing the intermediate stock and the target scion after grafting. The rooting powder is used for treating the intermediate stock and the target scion, and the transpiration inhibitor is used for treating the intermediate stock, so that the grafting survival rate is significantly improved; the intermediate stock is used for secondary grafting, the problem of incompatibility of direct grafting of the tree-shaped Chinese rose is solved, the problems of a partial crown and a small crown width are solved, and the crown width is rapidly increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of seedling breeding, in particular to a method for grafting tree-shaped Chinese rose by using intermediate stock. BACKGROUND

[0002] Chinese rose, a plant of Rosaceae-Rosa, is favored by people because of its beautiful flower, various colors, fragrance, and constant blooming in four seasons. Wild Rosa plants such as Rosa davurica and Rosa multiflora are shrub-like liana plants, which have the advantages of rich resources, strong stem, strong root activity, and fast growth, and are excellent rootstocks for grafting Chinese rose.

[0003] At present, domestic tree-shaped Chinese rose is mostly grafted by using local wild Rosa plants as rootstocks to make up for the shortage of Chinese rose cutting seedlings and improve the overall ornamental value and value. However, due to the fact that modern Chinese rose rarely or never introduces the gene of domestic local wild Rosa plants in the process of breeding and crossing, the kinship between a small part of modern Chinese rose and local wild Rosa plants is relatively far, and obvious incompatibility rejection phenomenon such as unhealed callus and poor growth of scion branches after grafting occurs. Therefore, although the local Rosa plant resources are rich and have excellent adaptability, a small part of Chinese rose varieties cannot be successfully grafted, so that the local wild Rosa plant resources have not been fully utilized.

[0004] Intermediate stock refers to a section of rootstock between scion and base stock. The intermediate stock grafting method is mainly applied to economic crops such as fruit trees. By grafting the intermediate stock first, and then grafting the target scion on the intermediate stock after the survival of the intermediate stock, the resistance and quality of the fruit trees can be improved or modified. In the field of flower plants, especially in the field of Rosa plants, the application of intermediate stock is very rare, and there is no related report on the method of grafting both intermediate stock and target scion at the same time.

[0005] In addition, due to insufficient light or lack of bud points in a certain direction during the growth of Rosa plants, tree-shaped Chinese rose may have the problems of uneven crown or small crown width, which affects the appearance and reduces the overall ornamental value and value. SUMMARY

[0006] Therefore, the present application aims to provide a method for grafting tree-shaped Chinese rose by using intermediate stock to solve the above problems.

[0007] To achieve the above-mentioned purpose, the technical scheme of the present application is as follows:

[0008] A method for grafting tree-shaped Chinese rose by using intermediate stock, the method comprising the following steps:

[0009] 1) Cultivating Rosa base stock: cutting healthy semi-lignified or lignified local Rosa branches, and then performing the operations of seedling, inclined artificial planting, planting, leaving sacrifice branches, branch bending, and branch bud pruning to obtain Rosa base stock;

[0010] 2) The intermediate stock is selected from rose branches with smaller diameter than the base stock and affinity with both the base stock and the target scion;

[0011] 3) Before grafting, all lateral buds on the intermediate stock branches are removed, and the intermediate stock branches and target scion branches are soaked in 0.08-0.1 g / L rooting powder for 3 hours, and kept moist and dry;

[0012] 4) When grafting, the intermediate stock branches are soaked in 900-1000 times of transpiration inhibitor for 3-5 seconds before being removed;

[0013] 5) The embedded bud grafting method is adopted, the target scion is first grafted to the top of the intermediate stock branch that has been cut, and the target scion and the intermediate stock branch are wrapped from top to bottom with stretchable glutinous rice paper until the oblique cut at the bottom of the intermediate stock branch; then the intermediate stock branch with the grafted target scion is grafted to the rose base stock using the wedge grafting or cutting grafting method or the embedded bud-vent grafting method;

[0014] 6) After grafting, the intermediate stock and the target scion need to be reinforced, and the buds on the base stock branches at other positions need to be removed in time; if there are individual target scions with stronger or weaker growth, the intermediate stock branches with stronger growth need to be girdled to control the balance of nutrient transport; in addition, water and fertilizer need to be supplemented in time and sterilized.

[0015] Further, in step 2), the intermediate stock is selected from "Y" type or "r" type; in step 4), after soaking the transpiration inhibitor, the bifurcation of the "Y" type or "r" type branch is vertically cut into a smooth oblique cut, which is used as the intermediate stock of the base stock, and its length is the same as the height of the other target scion grafting point of the base stock; in step 5), the embedded bud-vent grafting method is adopted to graft to the main stem of the rose base stock.

[0016] Further, in step 2), the branches of the rose base stock are pruned to the same height; in step 5), the wedge grafting or cutting grafting method is adopted to graft to the top of the branches of the rose base stock.

[0017] Further, the grafting time is preferably selected in the spring and autumn seasons in the south or the spring season in the north.

[0018] Further, if there is obvious bleeding, the base stock grafting site below 3-5 cm needs to be girdled before grafting in the spring when the water and fertilizer conditions are sufficient.

[0019] Further, the step of cultivating the rose base includes: the method of seedling is that: cutting 15-20cm healthy semi-lignified or lignified local rose branches, soaking in 0.1-0.2g / L rooting powder for 40-60 minutes, and then inserting into the seedbed at an angle of 30-45°, and ensuring that 2-3 bud points of the cutting branches are buried in the soil; after survival and new branch growth, large water and fertilizer management is needed, and no pruning is needed during the period; when the new branches grow to semi-lignified and naturally droop, the new branches are twisted and pressed down around the base, breaking the apical dominance, promoting the nutrients to flow back to the roots to grow new shoots, leaving 2-3 healthy and thick shoots to pull and fix, so that they grow vertically.

[0020] Further, the method of inclined planting is that: cutting the lignified shoot at the base, smoothing the cutting surface of the main stem, cutting the overgrown side branches, and digging out all side buds 10-15cm away from the cutting surface; after soaking the main stem 10-15cm in 0.2-0.3g / L rooting powder for 40-80 minutes, inserting it into a non-woven fabric seedling bag, and then planting it in a shady place at an angle of 0-30°, pouring rooting water, and then righting it after rooting.

[0021] Further, the method of planting is that: the planting hole is 25-30cm deep and wide, 10-15cm thick fermented farmyard manure is placed at the bottom of the hole as basal fertilizer, and 3-5cm of hole soil is backfilled to completely cover the basal fertilizer; the non-woven fabric bag is vertically cut around, planted into the hole together with the bag, supported and fixed with a stick, backfilled with hole soil, and ensured that all bud points of the main stem are exposed to the ground, and then poured with rooting water; thereafter, water is poured in time according to the weather, and 5-10g of fertilizer is applied to each plant each time, and urea and compound fertilizer are alternately applied.

[0022] Further, the method of leaving sacrifice branches is that: the side branches growing from the base of the rose main stem are used as sacrifice branches, and they are allowed to grow naturally to speed up the thickening of the part below the branch point of the main stem.

[0023] The method of pressing branches is that: after the sacrifice branches grow to naturally droop, the sacrifice branches are twisted and pressed down at the base, breaking the apical dominance of the sacrifice branches, and making the nutrients of the sacrifice branches flow back to the main stem.

[0024] Further, the method of pruning buds is that: when the leaves of the twisted sacrifice branches turn yellow and fall off, the sacrifice branches are cut off; the repeated operation of leaving sacrifice branches, pressing branches, and pruning branches is carried out on the part below 3 / 5 of the height of the main stem; the side branches above 3 / 5 of the height of the main stem are used as branch cultivation of the main stem, and only sick branches and weak branches need to be cut off; when the diameter of the main stem reaches 1.8-2.5cm, all side bud points below 3 / 5 of the height of the main stem are dug out, and no sacrifice branches are left.

[0025] Compared with the prior art, the method for grafting tree-shaped Chinese rose by using intermediate stock has the following advantages:

[0026] 1、The present application significantly improves the overall grafting survival rate by using rooting powder to treat the intermediate stock and the target scion, and by using transpiration inhibitor to treat the intermediate stock.

[0027] 2、The present application solves the problem of incompatibility of direct grafting of tree-shaped Chinese rose, and the problems of small crown and small crown width, and realizes rapid increase of crown width.

[0028] 3、The present application reduces the water supply height of high pole branches after being separated from the body by using the method of inclined placement treatment of high pole branches, and improves the cutting survival rate.

[0029] 4、The present application promotes nutrient backflow to the main pole by leaving sacrifice branches, pressing branches and pruning branches treatment, speeds up the thickening of the main pole, and shortens the forming time. BRIEF DESCRIPTION OF DRAWINGS

[0030] The accompanying drawings, which form a part of this application, are intended to provide further understanding of the application and are incorporated herein for a purpose of explanations of the application and are not intended as an improper limitation of the application. In the drawings:

[0031] Figure 1 The branches grafted in Example 1;

[0032] Figure 2 The branches grafted in Example 2;

[0033] 1、Rosa chinensis rootstock; 2、Intermediate stock; 3、Target scion. DETAILED DESCRIPTION

[0034] It should be noted that the examples in the present application and the features in the examples can be combined with each other without conflict.

[0035] The present application will be described in detail below with reference to the examples.

[0036] Example 1 solves the problems of small crown, small crown width and incompatibility

[0037] A method for grafting tree-shaped Chinese rose by using intermediate stock, the method comprising the following steps:

[0038] 1、Cultivate Rosa chinensis rootstock: cut healthy semi-lignified or lignified local Rosa chinensis branches, and sequentially perform the operations of seedling raising, inclined planting, planting, leaving sacrifice branches, pressing branches and pruning buds to obtain Rosa chinensis rootstock 1;

[0039] 1) Seedling raising:

[0040] Mountain wood as grafting base in the growing season as experimental material, take healthy semi-lignified branches about 18 cm, only leave the top 1-2 pieces, and the morphological lower end of the cut is inclined to smooth. After soaking in 0.2g / L ABT rooting powder for 40 minutes, it is inclined at 30° and inserted into the seedbed, leaving 8-10 cm exposed to the ground, ensuring that more than 2 bud points of the branch are buried in the soil, and pouring thoroughly. After rooting, water and fertilizer management, fertilize once every ten days (urea and compound fertilizer are used alternately, 2-3g each time), to promote rapid growth of branches and leaves, and no pruning is needed during this period. When the new branches grow to semi-lignified and naturally droop, they are twisted and pressed down from the base, breaking the dominance of the top and promoting the growth of thick and strong shoots at the root. Leave the two strongest shoots and fix them with a bamboo pole to make them grow vertically along the pole. When the twisted and pressed down branches have yellowing and leaf loss, cut them off from the base. When the shoots grow to the appropriate height, top them to promote the growth of lateral branches at the top.

[0041] 2) Inclined planting:

[0042] Cut the shoots from the base, cut the top of the overgrown lateral branches, smooth the cut of the main stem, and dig out all the lateral buds 15 cm away from the cut to avoid growing lateral branches in the soil. The wound after digging the buds is also beneficial to rooting. Soak the base of the main stem below the dug buds in 0.3g / L ABT rooting powder for 60 minutes, then insert it into the non-woven fabric seedling bag (diameter 12 cm x height 20 cm) prepared with the substrate (river sand (40%) + coconut husk (30%) + red soil (25%) + chicken manure (2-3%) : wood ash (1-2%)), and place it in a cool place at an angle of about 10°. Normal watering, and continue to cultivate after rooting.

[0043] 3) Planting:

[0044] Dig a planting hole about 30 cm deep and wide, put about 15 cm thick fermented chicken manure as basal fertilizer at the bottom of the hole, and backfill about 3 cm of soil to completely cover the basal fertilizer. Cut the non-woven fabric bag vertically and plant it in the hole together with the bag, support it with a bamboo pole, backfill the soil and ensure that all the bud points of the main stem are exposed to the ground, and pour thoroughly. After that, water according to the weather, and fertilize once a month during the growing season (urea and compound fertilizer are used alternately), about 5g per plant each time.

[0045] 4) Leave sacrificial branches:

[0046] Mountain wood after planting will grow rapidly under the condition of sufficient fertilizer and water during the growing season, and the lateral branches growing from the base will be sacrificial branches that do not need to be pruned to grow naturally to speed up the thickening of the main stem.

[0047] 5) Pressing branches:

[0048] When the sacrificial branch grows to the natural drooping and bending, twist and press the base of the sacrificial branch to break the top dominance of the sacrificial branch, so that the nutrients of the sacrificial branch flow back to the main stem, and the main stem is thickened rapidly.

[0049] 6) Pruning the branches:

[0050] When the leaves of the twisted sacrificial branch turn yellow and fall off, cut the sacrificial branch. Repeat the operation of leaving sacrificial branches, pressing branches and pruning branches on the part below 3 / 5 of the height of the main stem (the golden ratio is 0.618:1) to promote the thickening of the main stem; the side branches of the part above 3 / 5 of the height of the main stem are cultivated as branches of the main stem. When the diameter of the main stem reaches 2 cm, all the side bud points below 3 / 5 of the height of the main stem are removed, and no sacrificial branches are left.

[0051] 2, the middle stock 2 is selected from the "Y" type or "r" type, and the diameter is smaller than the base stock, and the thornless rose branch is compatible with the base stock and the target scion 3. The Rosa chinensis is selected as the target scion 3.

[0052] 3, before grafting, remove all side bud points on the thornless rose (middle stock) branch, and soak the thornless rose branch and the Rosa chinensis branch in 0.1 g / L ABT rooting powder for 3 hours, and dry it.

[0053] 4, when grafting, soak the pruned thornless rose branch in 1000 times of transpiration inhibitor for 3 seconds and take it out; vertically cut the bifurcation of the "Y" type or "r" type branch into a smooth bevel, which is used as the middle stock of the grafting base stock, and the length is the same height as the grafting point of other target scions of the mountain wood.

[0054] 5, adopt the embedded bud grafting method, first graft the target scion to the top of the thornless rose branch which has been cut, and use the stretchable glutinous rice paper grafting tape to wrap the target scion and the thornless rose branch from top to bottom to the bevel at the bottom of the thornless rose branch; then graft the thornless rose branch with grafted target scion to the mountain wood main stem (base stock) by using the embedded branch-belly grafting method.

[0055] 6, after grafting, the middle stock and the target scion need to be reinforced, and the target scion needs to be pruned and topped in time after germination to ensure full overall crown; the bud of the stock on the mountain wood at other positions needs to be removed in time. If there are individual target scions with strong or weak growth in the follow-up, the branches with strong growth need to be girdled to control the balance of nutrient transport. In addition, water and fertilizer are supplemented in time and sterilized and disinfected.

[0056] As Figure 1 This embodiment uses the middle stock to solve the problems of uneven crown, small crown and incompatibility. The base stock is mountain wood, the middle stock is thornless rose, and the target scion is Rosa chinensis.

[0057] Example 2 solves the problem of incompatibility

[0058] A method for grafting tree rose by using intermediate stock, comprising the following steps:

[0059] 1. Culturing rose rootstock: cutting healthy semi-lignified or lignified local rose branches, and sequentially performing seedling, inclined artificial planting, planting, leaving sacrifice branches, bending branches and pruning branch buds to obtain rose rootstock 1;

[0060] 1) Seedling:

[0061] In the growing season, mountain wood is selected as the experimental material for grafting rootstock, and healthy semi-lignified branches are cut to about 18 cm, only 1-2 leaves at the top are left, and the morphological lower end is inclined and flattened. After being soaked in 0.2 g / L ABT rooting powder for 40 minutes, it is inclined at an angle of 30° and inserted into the seedling bed, leaving 8-10 cm exposed to the ground, ensuring that more than two bud points of the branch are buried in the soil, and pouring thoroughly to fix the roots. After rooting, large water and fertilizer management is performed, and fertilizer (urea and compound fertilizer are alternately used, 2-3 g each time) is applied once every ten days to promote rapid growth of branches and leaves, and pruning is not required during the period. When the new branches grow to be semi-lignified and naturally droop, they are twisted and bent at the base and pressed to the four sides to break the top dominance and promote the growth of thick and strong sprout branches at the root. The two thickest sprout branches are left and fixed with a bamboo pole to make them grow vertically along the pole. When the twisted and bent branches have withered, yellow and fallen leaves, they are cut off from the base. When the sprout branches grow to the appropriate height, they are topped to promote the growth of lateral branches at the top.

[0062] 2) Inclined artificial planting:

[0063] The sprout branches are cut off at the base, the top overgrown lateral branches are cut short, the main stem cut-off is flattened, and all lateral buds 15 cm away from the cut-off are dug out to avoid subsequent growth of lateral branches under the soil. The main stem is soaked in 0.3 g / L ABT rooting powder for 60 minutes, then inserted into the non-woven fabric seedling bag (diameter 12 cm x height 20 cm) prepared with the substrate (river sand (40%) + coconut husk (30%) + red soil (25%) + chicken manure (2-3%) + wood ash (1-2%)), and placed in a shady place at an angle of about 10°. Normal watering is performed, and after rooting, the sprout branches are straightened and continued to be cultured.

[0064] 3) Planting:

[0065] A planting hole with a depth and width of about 30 cm is dug, about 15 cm thick fermented chicken manure is placed at the bottom of the hole as basal fertilizer, and about 3 cm of hole soil is backfilled to completely cover the basal fertilizer. The non-woven fabric bag is vertically cut around and planted in the hole together with the bag, supported and fixed with a bamboo pole, the hole soil is backfilled, and the main stem base is ensured to have all bud points exposed to the ground, and the hole is poured with thorough rooting water. Thereafter, water is timely poured according to the weather, and fertilizer (urea and compound fertilizer are alternately used) is applied once a month during the growing season, about 5 g per plant each time.

[0066] 4) Leave the sacrifice branch:

[0067] The mountainwood will grow rapidly under the condition of sufficient water and fertilizer in the growing season. The side branches growing from the base of the mountainwood are used as sacrifice branches, which are allowed to grow naturally without pruning, in order to speed up the thickening of the main stem.

[0068] 5) Press the branch:

[0069] When the sacrifice branch grows to the point of natural drooping and bending, twist and press the branch at the base of the sacrifice branch, break the top dominance of the sacrifice branch, and make the nutrients of the sacrifice branch flow back to the main stem, so as to speed up the thickening of the main stem.

[0070] 6) Prune the branch bud:

[0071] When the leaves of the twisted sacrifice branch turn yellow and fall off, cut the sacrifice branch. Repeat the operation of leaving the sacrifice branch, pressing the branch, and pruning the branch on the part below 3 / 5 of the height of the main stem (the golden ratio is 0.618:1) to promote the thickening of the main stem; the side branches above 3 / 5 of the height of the main stem are used as branches of the main stem. When the diameter of the main stem reaches 2 cm, all the side bud points below 3 / 5 of the height of the main stem are removed, and the sacrifice branch is not left.

[0072] 2, prune the branches (base stock) of mountainwood to the same height, and select straight stem thornless rose (intermediate stock) branches with smaller diameter than the base stock and compatible with the base stock and target scion as the intermediate stock 2, all thornless rose branches are of the same length.

[0073] 3, before grafting, remove all side bud points on the thornless rose branches, and soak the thornless rose branches and the Rosa roxburghii Tratt branches in 0.1 g / L ABT rooting powder for 3 hours, keep moist and dry.

[0074] 4, when grafting, soak the pruned thornless rose branches in 1000 times transpiration inhibitor for 3 seconds and then take them out.

[0075] 5, use the embedded bud grafting method, first graft the target scion to the top of the thornless rose branch that has been cut, and use stretchable glutinous rice paper grafting tape to wrap the target scion and the thornless rose branch from top to bottom to the oblique cut at the bottom of the thornless rose branch; then use the wedge grafting or cutting method to graft the thornless rose branch with the grafted target scion to the top of the branch (base stock) of mountainwood.

[0076] 6, after grafting, the intermediate stock and the target scion need to be reinforced, and the buds of the target scion need to be pruned and topped in time to ensure a full and lush overall crown; the buds of the stock on other positions of mountainwood need to be removed in time. If there are individual target scions with stronger or weaker branches in the follow-up, the branches with stronger growth need to be girdled to control the balance of nutrient transport. In addition, water and fertilizer need to be supplemented in time and sterilized.

[0077] As Figure 2 In order to solve the incompatibility problem by using the intermediate stock method in the present embodiment, the stock is mountain wood, the intermediate stock is thornless rose, and the target scion is moon powder.

[0078] Therefore, when encountering rose branches with uneven crown and small crown width, the method of the present application can achieve rapid crown width increase through the intermediate stock; when encountering incompatibility rose branches, the method of the present application can achieve normal growth of the stock and the target scion through the intermediate stock; when encountering rose branches with uneven crown, small crown width and incompatibility, the method of the present application can achieve rapid crown increase and normal growth of the target scion through the intermediate stock.

[0079] In order to verify the feasibility and advantages of the method of the present application, a series of comparative examples are set up for comparison. The experimental sites of the embodiments and comparative examples of the present application are in Zhaoqing City, Guangdong Province, where there are wild mountain wood resources, and the introduced thornless roses can grow normally throughout the year in this area. Mountain wood is used as the stock, thornless rose is used as the intermediate stock, and moon powder is used as the target scion.

[0080] Influence of inclination of simulated planting angle on comparative example 1

[0081] About 150 cm of mountain wood branches were obtained by seedling culture, and were simulated planted at different inclinations (0°, 30°, 45°, 60°, 90°), with 8 replicates for each treatment. The treatment time was 2 months (Table 1), and other processes were the same.

[0082] Table 1: Experimental results of simulated planting at different inclinations

[0083]

[0084]

[0085] From the results in Table 1, it can be seen that the survival number of simulated planting at 0° and 30° inclination is the highest, and the effect is the best, while the mortality rate of vertical cutting is the highest. The main reason is that the main stem of simulated cutting is high, and soaking with rooting powder can improve the survival rate to a certain extent, but the water supply of the branch after being separated is too high, and there is still a high mortality rate. The surviving plants also have the phenomenon of top pruning.

[0086] Influence of leaving sacrifice branches, pressing branches and pruning branches on comparative example 2

[0087] The mountain wood plants obtained after simulated planting were planted, and healthy mountain wood plants with similar growth vigor were selected, with 5 replicates for each treatment. The treatment time was 8 months, and the base diameter and its growth performance at 0, 3, 5 and 8 months were observed.

[0088] Treatment 1: After planting, 3 / 5 or more of the lower part of all lateral bud points were dug out, and no leaving sacrifice branches, pressing branches or pruning branches were treated;

[0089] Treatment 2: After planting, leave the sacrificial branches, do not do the branch pressing and pruning treatment;

[0090] Treatment 3: After planting, do the treatment of leaving sacrificial branches, branch pressing and pruning.

[0091] Table 2: Changes in base diameters of different treatments

[0092]

[0093] After 8 months of growth, the base diameters of the plants in each treatment changed, among which the growth rate of treatment 1 was the slowest. This was mainly because the bud points below 3 / 5 of the main stem height of A. franchetii were removed, which could not grow lateral branches from the base to increase biomass, resulting in slow growth. Treatment 2 left sacrificial branches, but did not do branch pressing and pruning treatment, and nutrients were preferentially supplied to the base lateral branches, causing the base lateral branches to grow disorderly, resulting in an unobvious base main stem, strong lateral branch growth, and weak top growth. Treatment 3 did the treatment of leaving sacrificial branches, branch pressing and pruning, and its main stem was obvious, with fast thickening speed and natural branching.

[0094] Influence of Comparative Example 3 Inhibition Transpiration Agent and Rooting Powder

[0095] Incompatible grafting experiments were conducted on the cultured A. franchetii, with 10 replicates for each treatment (taking the middle stock in each grafting as one replicate), and the treatment time was 2 months.

[0096] Treatment 1: After soaking the YUEYUE powder (target scion) with 0.1 g / L ABT rooting powder, directly grafting it to A. franchetii (base stock).

[0097] Treatment 2: Without any agent treatment, directly grafting the middle stock and target scion;

[0098] Treatment 3: Without agent treatment for the target scion, using 1000 times inhibition transpiration agent (Guangguang · Inhibition Transpiration) for the middle stock;

[0099] Treatment 4: Using 0.1 g / L ABT rooting powder for the target scion, without agent treatment for the middle stock;

[0100] Treatment 5-1: Using 0.1 g / L ABT rooting powder for the middle stock and target scion, using 800 times inhibition transpiration agent (Guangguang · Inhibition Transpiration) for the middle stock;

[0101] Treatment 5-2: Using 0.1 g / L ABT rooting powder for the middle stock and target scion, using 900 times inhibition transpiration agent (Guangguang · Inhibition Transpiration) for the middle stock;

[0102] Treatment 5-3: Using 0.1 g / L ABT rooting powder for the middle stock and target scion, using 1000 times inhibition transpiration agent (Guangguang · Inhibition Transpiration) for the middle stock;

[0103] Treatment 5-4: the intermediate stock and the target scion were treated with 0.1 g / L ABT rooting powder, and the intermediate stock was treated with 1100 times of transpiration inhibitor (Guoguang · Anti-transpiration);

[0104] Treatment 5-5: the intermediate stock and the target scion were treated with 0.1 g / L ABT rooting powder, and the intermediate stock was treated with 1200 times of transpiration inhibitor (Guoguang · Anti-transpiration).

[0105] Table 3: Survival rate of intermediate stock and target scion

[0106]

[0107] The experimental results of each treatment are shown in Table 3. Treatment 1 has a low grafting survival rate due to incompatibility between the month powder (target scion) and the mountain incense (base stock). Although a small number of grafts survived in the early stage (first month), the growth of the scion was weak, the callus at the grafting site swelled into a tumor, and the scion died in the later stage (second month). Treatment 2 introduced an intermediate stock, but did not use any chemicals on the intermediate stock and the target scion. Therefore, the intermediate stock branches died of dehydration soon after grafting, and the target scion also died. Treatment 3 introduced an intermediate stock and used a transpiration inhibitor on it, which kept the survival rate of the intermediate stock very high (80.0%) in the early stage. However, the survival rate of the target scion was not high (50.0%) because no rooting powder was used on it. In addition, the intermediate stock had no bud points (all bud points were removed before grafting), so nutrients could not be supplied upward. Therefore, the intermediate stock that survived in the early stage gradually withered and died in the later stage. Treatment 4 used rooting powder on the target scion, but the intermediate stock died of dehydration soon after grafting because it was not treated. Therefore, the overall survival rate was not high. Treatment 5 used different concentrations of transpiration inhibitors on the intermediate stock (the same concentration of rooting powder was used on the intermediate stock and the target scion). The overall survival rate was relatively high, and the results of using 900 and 1000 times of transpiration inhibitor were the best.

[0108] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method for grafting tree roses using an intermediate stock, characterized by: The method comprises the following steps: 1) Cultivate rose base stock: cut healthy semi-lignified or lignified local rose branches, and sequentially perform seedling raising, slanting false planting, planting, sacrificing branch retaining, branch pressing, and branch bud pruning to obtain rose base stock; The slanting false planting method is as follows: cut off the lignified shoot branch from the base, smooth the cut end of the main stem, cut off the overgrown side branches, and remove all side buds at 10-15 cm from the cut end; after the main stem is immersed in 0.2-0.3 g / L rooting powder for 40-80 min, the main stem is inserted into a non-woven fabric seedling raising bag, and the seedling raising bag is slanted to 0-30° and planted in a cool place, and then fixed with rooting water; after rooting, the main stem is righted; The sacrificing branch retaining method is as follows: the side branches growing from the base of the rose main stem are used as sacrificing branches, and the sacrificing branches are allowed to grow naturally to accelerate the thickening of the part below the branch growing point of the main stem; The branch pressing method is as follows: after the sacrificing branches grow to naturally droop, the sacrificing branches are twisted and pressed downward at the base, so that the top dominance of the sacrificing branches is broken, and the nutrients of the sacrificing branches are returned to the main stem; The branch bud pruning method is as follows: when the leaves of the twisted sacrificing branches turn yellow and fall off, the sacrificing branches are cut off; the sacrificing branch retaining, branch pressing, and branch pruning are repeatedly performed on the part below 3 / 5 of the height of the main stem; the branches above 3 / 5 of the height of the main stem are used as branch culture of the main stem, and only the sick branches and weak branches are cut off; when the diameter of the main stem reaches 1.8-2.5 cm, all side bud points below 3 / 5 of the height of the main stem are removed, and no sacrificing branches are retained; 2) The intermediate stock is selected from rose branches with a smaller diameter than the base stock and affinity with the base stock and target scion; the intermediate stock is selected in a "Y" type or "r" type; 3) Before grafting, all side bud points on the intermediate stock branch are removed, and the intermediate stock branch and the target scion branch are immersed in 0.08-0.1 g / L rooting powder for 3 h, and then dried; 4) During grafting, the pruned intermediate stock branch is immersed in 900-1000 times of transpiration inhibitor for 3-5 s, and then taken out; after being immersed in the transpiration inhibitor, the "Y" type or "r" type branch is vertically cut into a smooth bevel at the branch fork, which is used as the intermediate stock of the base stock, and the length of the intermediate stock is the same as that of the other target scion grafting point of the base stock; 5) The embedded bud grafting method is adopted, the target scion is first grafted to the top end of the cut intermediate stock branch, and the target scion and the intermediate stock branch are wrapped from top to bottom to the bevel at the bottom of the intermediate stock branch with stretchable glutinous rice paper grafting tape; then, the intermediate stock branch with the grafted target scion is grafted to the main stem of the rose base stock by the embedded branch-belly grafting method; 6) After grafting, the intermediate stock and the target scion need to be reinforced, and the buds of the target scion are pruned and topped in time to ensure a full overall crown; the buds of the base stock on the stem of the rose base stock are removed in time; if there are individual target scions with stronger or weaker branches in the subsequent process, the intermediate stock branches with stronger growth are ringed to control the balance of nutrient transport; in addition, water and fertilizer are supplemented in time, and sterilization and disinfection are performed.

2. The method for grafting tree rose using intermediate stock according to claim 1, characterized in that: In step 2), the branches of the rose base stock are pruned to the same height; in step 5), the branches are grafted to the top end of the branches of the rose base stock by the split grafting or cutting grafting method.

3. The method for grafting tree rose using intermediate stock according to claim 1, wherein: The grafting time is preferably selected in the spring and autumn of the south or the spring of the north at 15-25℃.

4. The method for grafting tree rose using intermediate stock according to claim 1, wherein: If there is obvious bleeding, the grafting should be performed after 3-5 cm of the grafting part of the rootstock is girdled the day before.

5. The method for grafting tree rose using intermediate stock according to claim 1, wherein: The steps for cultivating the Rosa chinensis rootstock include: the seedling method is to cut 15-20 cm of healthy semi-lignified or lignified local Rosa chinensis branches, soak them in 0.1-0.2 g / L rooting powder for 40-60 minutes, and then insert them into the seedling bed at an angle of 30-45°, and make sure that 2-3 bud points of the cuttings are buried in the soil; after survival and new branch growth, large water and fertilizer management is required, and no pruning is needed during the period; when the new branches grow to be semi-lignified and naturally droop, twist and press them down from the base, break the apical dominance, promote the nutrients to flow back to the roots to grow new shoots, leave 2-3 healthy and strong shoots to pull and fix, and make them grow vertically; when the shoots grow to a certain height, cut the new branches from the base, and top the shoots to promote the growth of lateral branches.

6. The method for grafting tree rose using intermediate stock according to claim 1, wherein: The planting method is: the planting pit is 25-30 cm deep and wide, 10-15 cm thick fermented farmyard manure is put at the bottom of the pit as basal fertilizer, 3-5 cm of pit soil is backfilled to completely cover the basal fertilizer; the non-woven fabric bag is vertically cut around, and is planted into the pit together with the bag, supported and fixed with a rod, the pit soil is backfilled, and make sure that all bud points of the main rod are exposed to the ground, and pour thorough rooting water; thereafter, water is poured in time according to the weather, and fertilizer is applied once a month in the growing season, 5-10 g of fertilizer is applied to each plant each time, and urea and compound fertilizer are applied alternately.

Images