In-situ rapid propagation method for annual production of clumping bamboo seedlings in factory

Abstract

The application discloses an in-situ rapid propagation method for factory annual production of clump bamboo seedlings, which comprises the following steps: greenhouse greenhouse planting and fertilizer and water management of mother bamboo, so that the mother bamboo is in a suitable environment and grows at a high speed throughout the year; mother plant selection and top end advantage removal; in-situ rapid propagation position treatment, new plant formation and new plant transplanting; and repeated propagation is carried out in batches. The application controls the growth environment of bamboo artificially, so that the bamboo grows at a high speed throughout the year, annual bamboo seedling production can be carried out, the bamboo shoots damaged by the weevils can be kept from retreating, and new plants can be produced by rooting and proliferation. The rooting effect of the application has a time sequence on the same mother bamboo, that is, the upper nodes can be transplanted after rooting, and the lower nodes can be quickly activated and rooted. The method has the advantages of simple technology, convenient operation, low cost, high rooting rate and high survival rate, and is an important means for rapid propagation of bamboo seedlings.

Description

Technical Field

[0001] This invention belongs to the field of plant propagation technology, and more specifically, this invention relates to an in-situ rapid propagation method for the year-round industrial production of clump-forming bamboo seedlings. Background Technology

[0002] Bamboo primarily reproduces through horizontally growing underground rhizomes (commonly known as bamboo whips). These rhizomes are slightly hollow in the middle, with numerous and dense nodes, from which many fibrous roots and buds grow. Some buds develop into bamboo shoots, emerging from the ground to grow into bamboo, while others do not emerge but grow horizontally, developing into new underground rhizomes. Clump-forming bamboos include those within genera such as *Bambusa* and *Bambusa*, represented by species like *Bambusa textilis*, *Bambusa ventricosa*, *Bambusa cirrhosa*, and *Bambusa serrata*. Scattered bamboos include those in the genus *Bambusa*, represented by species like *Bambusa purpurea*, *Bambusa squareis*, *Bambusa mosois*, and *Bambusa purpurea*. Mixed-growth types include those in the genus *Bambusa glabra*, represented by species like *Bambusa chinensis* and *Bambusa purpurea*.

[0003] Take Dendrocalamus brandisii (Munro) Kurz. as an example: it is a tree-like bamboo plant belonging to the Poaceae family and the Dendrocalamus genus. A clump-forming bamboo, with culms reaching up to 15 meters in height, drooping or even long-drooping at the tip, and no raised culm rings; culm sheaths fall off early, leathery, with small auricles; ligule with deep serrations on the upper margin; culm blades reflexed or nearly erect, leaf sheaths covered with small white bristles; leaf size varies; flowering branches are whip-like, internodes 2.5–3.8 cm long, flattened on one side, densely covered with rusty-brown soft hairs; each node of the flowering branch bears clusters of pseudo-spikelets, spikelets ovoid, purplish-brown, with blunt tips, containing small florets; lemma resembling glumes, palea with cilia on the keel; anthers greenish-yellow, filaments short; ovary ovoid, covered with hairs, stigma purple, feathery; fruit ovoid, pericarp hard and shell-like. Distributed from east to west in southern Yunnan, China, widely cultivated near villages and hamlets; also found in Myanmar, Laos, Vietnam, and Thailand; cultivated in India. Its main propagation methods include:

[0004] Bamboo culm propagation: In sparse bamboo forests with flat terrain and fertile soil, select the edge of the bamboo clump with good light penetration. From February to April, select 1-2 year old, robust bamboo culms with plump dormant buds as mother bamboo. Dig a horizontal trench about 15-20 cm deep and wide from the base of the mother bamboo. Make a notch on the back of the base of the bamboo culm at the location of rapid in-situ propagation, reaching 2 / 3 of the depth of the culm. Then, slowly bend the bamboo culm towards the trench, leaving about 20 nodes. Cut off the bamboo tip, leaving the last node with leaves. Cut off all other branches except for 2-3 nodes of the main branch and dormant buds from the base. Press the mother bamboo culm into the trench, cover it with 3-5 cm of soil, gently compact it, leaving the last node with leaves exposed, water it, and cover it with straw.

[0005] Cultivation by burying the original culm: This method is similar to burying the original bamboo culm, except that the mother bamboo culm is separated from the bamboo clump and moved to the nursery for seedling cultivation. On the prepared seedbed, make parallel trenches every 20-25 cm, with a width and depth of about 12-15 cm. The trenches should be appropriately wider and deeper at the burial site of the bamboo stump. Then, apply mud slurry to the mother bamboo stump, place it flat in the trench with the cut end of the stump facing down and the buds facing to the sides. Cover with about 3 cm of soil, slightly thicker at the stump, compact it, and water thoroughly. Finally, cover with straw.

[0006] Stem-burying propagation: Cut the selected mother bamboo at ground level from the base, without the stubble. The rest of the process is the same as for stem-burying propagation. To encourage dormant buds in each node to sprout and root, make two rings 8-10 cm above each node of the original stem, reaching the green part of the bamboo, or make a small hole. In early autumn, after most of the bamboo seedlings have rooted, cut the rooted nodes at the original rings or holes, allowing them to grow into independent bamboo clumps for direct use in landscaping. Alternatively, they can be divided and transplanted for further propagation.

[0007] Existing propagation methods suffer from low seedling efficiency, high cost, and low survival rate. For example, in the paper "Optimization of Asexual Propagation Techniques for Three Bamboo Plants," Zhang Baosheng et al. used stalk-burying and division techniques to propagate *Phyllostachys pubescens*, achieving a survival rate of up to 96%. In "Discussion on Asexual Propagation Techniques of *Phyllostachys pubescens* in Yunnan" (Modern Agricultural Science and Technology, No. 22, 2013), Chen Heyan et al. used stalk-burying and cutting techniques to propagate *Phyllostachys pubescens*, achieving a survival rate of up to 82.5%. In "Optimization of Rapid Propagation Techniques for *Phyllostachys pubescens* by Cuttings" (Hubei Forestry Science and Technology, Vol. 53, No. 5, October 2023), Yang Zhi used rapid propagation techniques by cuttings, adjusting the fungicide treatment time, cutting age, and different plant growth regulators, achieving a rooting rate of up to 84.7% for *Phyllostachys pubescens* cuttings.

[0008] Therefore, researching a new method for propagating clump-forming bamboo, which can enable the year-round industrial production of clump-forming bamboo seedlings and improve the rooting rate and survival rate of clump-forming bamboo, has important application value. Summary of the Invention

[0009] One object of the present invention is to solve at least the above-mentioned problems and / or defects, and to provide at least the advantages described below.

[0010] To achieve these objectives and other advantages of the present invention, an in-situ rapid propagation method for the year-round industrial production of clump-forming bamboo seedlings is provided, comprising the following steps:

[0011] Step 1: Select healthy one-year-old mother bamboo, transplant them into greenhouses, and provide fertilizer and water management to ensure that the mother bamboo is in a suitable environment throughout the year.

[0012] Step 2: Select healthy, disease-free, and vigorous current-year or one-year-old mother bamboo that has not yet stretched its branches and leaves, and cut it at a height of 2.5 to 3.5m to remove apical dominance;

[0013] Step 3: Use the next or two nodes below the cut point as the location for in-situ rapid propagation. Moisten the perlite and humus with rooting powder solution, make a mud ball, wrap it around the in-situ rapid propagation location, wrap it tightly with polyethylene plastic film, and tie it with packaging rope.

[0014] Step 4: Observe whether the in-situ rapid propagation site has taken root. Keep the soil ball moist during this period. When the new roots are growing vigorously, cut off the in-situ rapid propagation site after rooting. Cut off the main branch with 3 nodes and plant it in a container filled with substrate. Water it thoroughly to settle the roots and then transplant it.

[0015] Step 5: Repeat steps 3 and 4 at the next segment after the in-situ rapid propagation site cut in step 4 to propagate the next batch.

[0016] Preferably, in step one, the environmental requirements for the greenhouse are: equipped with a temperature, humidity, and light control system to maintain a temperature of 18–25°C, an air humidity of 60%–75%, and a light intensity of 10,000–20,000 μmol / (s·m²). 2 The interior is lined with a 1-meter-thick layer of loose, breathable, and fertile sandy soil.

[0017] Preferably, in step three, the rooting powder solution is an ABT1 solution with a concentration of 180–220 mg / L.

[0018] Preferably, in step three, the volume ratio of perlite to humus is 1.5 to 2.5:1.

[0019] Preferably, in step three, the perlite and humus are pretreated as follows: they are stirred and sterilized with 500 times their weight of 75-85% wettable carbendazim powder, and nutrient solution is added to maintain the humidity so that they can be clump together without falling apart.

[0020] Preferably, the nutrient solution comprises the following components: potassium nitrate 1900 mg / L, ammonium nitrate 1650 mg / L, potassium dihydrogen phosphate 170 mg / L, magnesium sulfate 370 mg / L, calcium chloride 440 mg / L, potassium iodide 0.83 mg / L, boric acid 6.2 mg / L, manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, sodium molybdate 0.25 mg / L, copper sulfate 0.025 mg / L, cobalt chloride 0.025 mg / L, inositol 100 mg / L, glycine 2 mg / L, thiamine hydrochloride 0.1 mg / L, pyridoxine hydrochloride 0.5 mg / L, nicotinic acid 0.5 mg / L, disodium EDTA 37.3 mg / L, ferrous sulfate 27.8 mg / L, with the remainder being water.

[0021] Preferably, in step three, the volume of the mud ball is 1-2L; the upper part of the polyethylene plastic film has a small opening or the broken end of the bamboo stem is exposed, and the lower part has 3-4 small holes.

[0022] Preferably, step three is performed on a cloudy day or in the evening on a sunny day.

[0023] Preferably, in step four, vigorous growth of new roots is observed on days 40 to 50.

[0024] Preferably, in step four, the container is a container with a diameter of 25cm and a height of 25cm, and the substrate is soil; the transplanting management is as follows: cover the seedbed with a 60-80% shade net, water intermittently, and keep the soil moist.

[0025] Preferably, in step four, if weevil infestation is observed during the rooting period at the in-situ rapid reproduction site, the following treatment is performed: cut the bamboo shoot at the segment below the weevil's feeding hole, clean out the weevil larvae, disinfect the cut with 75% ethanol, then wash it clean with water, and after slightly drying, use the 1st to 3rd segments below the cut as the in-situ rapid reproduction site, and repeat the operations of steps three and four for reproduction.

[0026] This invention offers at least the following beneficial effects: It discloses an in-situ rapid propagation method for year-round, factory-scale production of clump-forming bamboo seedlings. By artificially controlling the bamboo's growth environment, the bamboo maintains a high-speed growth state throughout the year, enabling year-round bamboo seedling production. The rooting effect of this invention exhibits a temporal sequence on the same mother bamboo; that is, after transplanting, nodes at higher positions that develop roots will rapidly activate and root at nodes at relatively lower positions. This invention not only prevents bamboo shoots affected by weevils from reverting to new shoots but also promotes rooting and proliferation to produce new plants. This invention primarily uses current-year bamboo shoots for year-round production, conducted in batches, resulting in excellent rooting effects, low management requirements, well-developed root systems, and a 100% rooting and transplant survival rate, superior to existing propagation methods. It is an important means of rapid bamboo seedling propagation.

[0027] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0028] Figure 1 The bamboo shoots damaged by weevils in Example 2;

[0029] Figure 2 In Example 2, the three segments below the truncated section were used as the location for in-situ rapid propagation.

[0030] Figure 3In Example 2, new roots grew from the in-situ rapid propagation site above the cut-off point 45 days later;

[0031] Figure 4 In Example 2, the in-situ rapid propagation site after rooting is cut off and then transplanted to become a new plant;

[0032] Figure 5 The new plant that sprouted two new shoots and several new branches three months after transplanting in Example 2;

[0033] Figure 6 In Example 2, new roots grew from the in-situ rapid propagation site in the middle two months after the pruning. Detailed Implementation

[0034] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0035] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0036] In the following examples, the nutrient solution used comprises the following components: potassium nitrate 1900 mg / L, ammonium nitrate 1650 mg / L, potassium dihydrogen phosphate 170 mg / L, magnesium sulfate 370 mg / L, calcium chloride 440 mg / L, potassium iodide 0.83 mg / L, boric acid 6.2 mg / L, manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, sodium molybdate 0.25 mg / L, copper sulfate 0.025 mg / L, cobalt chloride 0.025 mg / L, inositol 100 mg / L, glycine 2 mg / L, thiamine hydrochloride 0.1 mg / L, pyridoxine hydrochloride 0.5 mg / L, nicotinic acid 0.5 mg / L, disodium EDTA 37.3 mg / L, ferrous sulfate 27.8 mg / L, and the remainder is water.

[0037] In the following examples, the clump-forming bamboo used is *Phyllostachys brevis*.

[0038] Example 1

[0039] A method for the in-situ rapid propagation of clump-forming bamboo seedlings for year-round industrial production includes the following steps:

[0040] Step 1: Select healthy one-year-old mother bamboo, divide them into clumps, and transplant them into a greenhouse. Provide appropriate fertilizer and water management to ensure the mother bamboo is in a suitable environment year-round. The greenhouse environment must be equipped with a temperature, humidity, and light control system, maintaining a temperature of 20℃, air humidity of 70%, and light intensity of 15000 μmol / (s·m²). 2 The interior is lined with a 1-meter-thick layer of loose, breathable, and fertile sandy soil.

[0041] Step 2: Select healthy, disease-free, and vigorous current-year bamboo shoots with unextended branches and leaves, and cut them at a height of 3m to remove apical dominance;

[0042] Step 3: Using the section immediately following the cut as the site for in-situ rapid propagation, the operation is carried out on a sunny evening. Moisten perlite and humus with a 200 mg / L ABT1 solution (perlite to humus volume ratio 2:1), forming a mud ball to wrap the in-situ rapid propagation site. The wrapping volume is 1.5L. Wrap the outside tightly with polyethylene plastic film and secure with packaging rope. Make a small opening at the top of the polyethylene plastic film and leave four small holes at the bottom to allow rainwater to flow in. The perlite and humus are pre-mixed and sterilized with 500 times their weight of 80% wettable carbendazim powder, and nutrient solution is added to maintain humidity so that they can be tightly clumped together without crumbling.

[0043] Step 4: Observe whether the in-situ rapid propagation site has taken root. Keep the soil ball moist during this period. On the 45th day, if the new roots are growing vigorously, cut off the in-situ rapid propagation site after rooting. Cut off the main branch with 3 nodes and plant it in a container with a diameter of 25cm and a height of 25cm filled with soil. Water it thoroughly to settle the roots. Cover the seedbed with a 70% shade net and water intermittently thereafter to keep the soil moist.

[0044] Step 5: Repeat the operations of Step 3 and Step 4 at the next segment after the in-situ rapid propagation site cut in Step 4 to carry out the next batch of propagation.

[0045] In this embodiment, the in-situ rapid propagation site showed good rooting effect, with a rooting rate and transplant survival rate of 100%.

[0046] Example 2

[0047] The difference between this embodiment and Embodiment 1 is that in step four, during the rooting period at the in-situ rapid propagation site, weevil infestation was observed, such as... Figure 1 As shown, the following treatment is performed: Cut the bamboo shoot below the feeding hole of the weevil, remove the weevil larvae, disinfect the cut surface with 75% ethanol, then wash it clean with water. After slightly drying, use the three segments below the cut as the in-situ rapid reproduction site, and repeat steps three and four for reproduction. Figure 2 As shown.

[0048] In this embodiment, materials that would otherwise die from weevil infestation regrow new roots and shoots 45 days after being cut, such as... Figure 3 As shown; cut off the rooted section at the point of rapid propagation, and then transplant it to become a new plant, as shown. Figure 4 As shown, three months after transplanting, two new shoots and several new branches emerged, as indicated. Figure 5 As shown; after 2 months, Figure 2New roots grow from the in-situ rapid propagation site in the middle, such as... Figure 6 As shown.

[0049] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A method for the in-situ rapid propagation of clump-forming bamboo seedlings for year-round industrial production, characterized in that, Includes the following steps: Step 1: Select healthy one-year-old mother bamboo, divide them into clumps, and transplant them into a greenhouse. Provide appropriate fertilizer and water management to ensure the mother bamboo is in a suitable environment year-round. The greenhouse environment requires: a temperature, humidity, and light control system, maintaining a temperature of 18-25℃, air humidity of 60%-75%, and light intensity of 10000-20000 μmol / (s·m²). 2 The interior is lined with a 1-meter-thick layer of loose, breathable, and fertile sandy soil. Step 2: Select healthy, disease-free, and vigorous current-year or one-year-old mother bamboo that has not yet stretched its branches and leaves, and cut it at a height of 2.5-3.5m to remove apical dominance; Step 3: Using the next or two nodes below the cut point as the in-situ rapid propagation site, moisten perlite and humus with rooting powder solution, form a mud ball, wrap it around the in-situ rapid propagation site, and tightly wrap it with polyethylene plastic film and tie it with packaging rope. The rooting powder solution is an ABT1 solution with a concentration of 180~220mg / L; the volume ratio of perlite to humus is 1.5~2.5:1; the perlite and humus are pretreated as follows: they are mixed and sterilized with 500 times their weight of 75~85% wettable carbendazim powder, and nutrient solution is added to maintain the humidity so that it can be tightly clumped without falling apart; the mud ball should have a volume of 1~2L; the polyethylene plastic film should have a small opening at the top or expose the bamboo stem cut end, and 3~4 small holes at the bottom. Step 4: Observe whether the in-situ rapid propagation site has taken root. Keep the soil ball moist during this period. When the new roots are growing vigorously, cut off the in-situ rapid propagation site after rooting. Cut off the main branch with 3 nodes and plant it in a container filled with substrate. Water it thoroughly to settle the roots and then transplant it. Step 5: Repeat steps 3 and 4 at the next segment after the in-situ rapid propagation site cut in step 4 to perform the next batch of propagation; In step four, if weevil infestation is observed during the rooting period at the in-situ rapid reproduction site, the following treatment should be performed: cut the bamboo shoot at the segment below the weevil feeding hole, clean out the weevil larvae, disinfect the cut with 75% ethanol, then wash it with clean water, and after slightly drying, use the 1st to 3rd segments below the cut as the in-situ rapid reproduction site, and repeat the operations of steps three and four for reproduction.

2. The in-situ rapid propagation method for year-round industrialized production of clump-forming bamboo seedlings as described in claim 1, characterized in that, The nutrient solution comprises the following components: potassium nitrate 1900 mg / L, ammonium nitrate 1650 mg / L, potassium dihydrogen phosphate 170 mg / L, magnesium sulfate 370 mg / L, calcium chloride 440 mg / L, potassium iodide 0.83 mg / L, boric acid 6.2 mg / L, manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, sodium molybdate 0.25 mg / L, copper sulfate 0.025 mg / L, cobalt chloride 0.025 mg / L, inositol 100 mg / L, glycine 2 mg / L, thiamine hydrochloride 0.1 mg / L, pyridoxine hydrochloride 0.5 mg / L, nicotinic acid 0.5 mg / L, disodium EDTA 37.3 mg / L, ferrous sulfate 27.8 mg / L, with the remainder being water.

3. The in-situ rapid propagation method for year-round factory production of clump-forming bamboo seedlings as described in claim 1, characterized in that, Step three is to be performed on a cloudy day or in the evening on a sunny day.

4. The in-situ rapid propagation method for year-round factory production of clump-forming bamboo seedlings as described in claim 1, characterized in that, In step four, vigorous growth of new roots was observed on days 40-50.

5. The in-situ rapid propagation method for year-round industrialized production of clump-forming bamboo seedlings as described in claim 1, characterized in that, In step four, the container is 25cm in diameter and 25cm in height, and the substrate is soil. The transplanting management is as follows: cover the seedbed with 60-80% shade netting, water intermittently, and keep the soil moist.

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