Traditional Chinese medicine composition for preventing and treating calf diarrhea, preparation method and application thereof

Abstract

The application belongs to the technical field of veterinary drugs, and specifically discloses a traditional Chinese medicine composition for preventing and treating diarrhea of calves, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: Bupleurum 5-10 parts, Paeonia 1-5 parts, Fructus Aurantii 1-5 parts, Alisma 1-5 parts, Atractylodes 5-10 parts, Astragalus 5-10 parts, Scutellaria 1-5 parts, Saposhnikovia 1-5 parts, Poria 1-5 parts, Pericarpium Citri Reticulatae 1-5 parts, Gardenia 1-5 parts, and Glycyrrhiza 1-5 parts. The traditional Chinese medicine composition provided by the application can be taken by the calf as soon as diarrhea occurs, helps the calf to supplement qi and consolidate the exterior, support the healthy qi and eliminate the pathogenic factors, and improve the immunity, and then, while the feeding quality is ensured, the traditional Chinese medicine composition has the effects of increasing the weight of the calf, improving anemia of the calf, improving the liver and kidney functions of the calf, and regulating and enhancing the immunity.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of veterinary medicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating diarrhea of calves and a preparation method and application thereof. BACKGROUND

[0002] Calves are the reserve force of pastures, and the health status and survival rate of calves seriously affect the development of the cattle industry. Diarrhea of calves is a common disease caused by bacteria, viruses or improper feeding management, and has the characteristics of complex pathogenesis, high morbidity and high mortality. It is the main cause of slow growth, poor development and death of calves. The disease is widely distributed and can occur at any time of the year, with a morbidity of about 80% of all calf diseases and a mortality of about 10%, and is known as the killer of newborn calves. Diarrhea of calves not only leads to the death of a large number of calves, but also increases the treatment cost of calves, causing huge economic losses to the cattle industry. In recent years, with the rapid development of the livestock breeding industry in China, the morbidity of diarrhea of calves has shown an increasing trend, which seriously hinders the healthy and rapid development of the dairy and beef cattle breeding industry in China.

[0003] At present, antibiotic drugs are the main drugs for treating diarrhea of calves, but due to the widespread use of antibiotics in the livestock breeding industry, the problems of increased drug resistance and drug residues caused by antibiotic drugs have also attracted widespread attention.

[0004] Traditional Chinese medicine has a long history in China and has played a huge role in the prevention and treatment of many diseases. In recent years, traditional Chinese medicine treatment has become a hot spot in the livestock breeding industry. Compared with antibiotics and other western medicines, traditional Chinese medicine has the characteristics of low toxicity, no drug resistance and less harm to the environment. Therefore, the development of a safe and effective traditional Chinese medicine prescription for treating diarrhea of calves will have important significance in the development of drugs for treating diarrhea of calves. SUMMARY

[0005] The purpose of the present application is to provide a traditional Chinese medicine composition for preventing and treating diarrhea of calves and a preparation method and application thereof, which effectively solves the problem of increased drug resistance caused by the use of antibiotic drugs to treat diarrhea of calves.

[0006] To solve the above technical problems, the technical solution adopted by the present application is as follows:

[0007] In a first aspect, the present application provides a traditional Chinese medicine composition for preventing and treating diarrhea of calves, which is prepared from the following weight parts of traditional Chinese medicine raw materials: Radix Bupleuri 5-10 parts, Paeoniae 1-5 parts, Fructus Aurantii 1-5 parts, Alisma orientalis 1-5 parts, Atractylodes 5-10 parts, Astragalus 5-10 parts, Scutellaria 1-5 parts, Saposhnikovia 1-5 parts, Poria cocos 1-5 parts, Pericarpium Citri Reticulatae 1-5 parts, Gardenia 1-5 parts, and Glycyrrhiza 1-5 parts.

[0008] Further, the Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Radix Bupleuri 5 parts, Paeoniae 4 parts, Fructus Aurantii Immaturus 5 parts, Alisma orientalis 3 parts, Atractylodes 5 parts, Astragalus 5 parts, Scutellaria 4 parts, Saposhnikovia 4 parts, Poria 3 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

[0009] Further, the Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Radix Bupleuri 5 parts, Paeoniae 3 parts, Fructus Aurantii Immaturus 4 parts, Alisma orientalis 4 parts, Atractylodes 6 parts, Astragalus 5 parts, Scutellaria 5 parts, Saposhnikovia 4 parts, Poria 4 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

[0010] Further, the Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Radix Bupleuri 5 parts, Paeoniae 3 parts, Fructus Aurantii Immaturus 4 parts, Alisma orientalis 4 parts, Atractylodes 6 parts, Astragalus 5 parts, Scutellaria 5 parts, Saposhnikovia 4 parts, Poria 4 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

[0011] Further, the Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Radix Bupleuri 5 parts, Paeoniae 3 parts, Fructus Aurantii Immaturus 4 parts, Alisma orientalis 4 parts, Atractylodes 6 parts, Astragalus 5 parts, Scutellaria 5 parts, Saposhnikovia 4 parts, Poria 4 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

[0012] Further, the dosage form of the Chinese medicine composition is powder or granular preparation.

[0013] In the second aspect, the application provides a preparation method of the Chinese medicine composition for preventing and treating diarrhea of calf, comprising the following steps: drying, crushing and sieving each Chinese medicine raw material in sequence, and then mixing them uniformly by weight to obtain the Chinese medicine composition.

[0014] Further, the Chinese medicine raw materials are taken by weight, and advanced extraction processes such as supercritical extraction or Chinese medicine decoction are used to prepare new decoction-free granular preparations for modernized cattle farms.

[0015] In the third aspect, the application provides the use of the Chinese medicine composition prepared by the preparation method of the second aspect or the Chinese medicine composition of the first aspect in the preparation of a medicine for preventing and treating diarrhea of calf.

[0016] Compared with the prior art, the application has the following beneficial effects:

[0017] (1) The Chinese medicine composition has no adverse effect on protein metabolism, lipid metabolism and liver function of the calf with diarrhea, effectively improves the cure rate of the calf with diarrhea, and helps the cow to correct the balance disorder caused in the transportation process.

[0018] (2) The traditional Chinese medicine composition provided by the application can be taken as soon as calf diarrhea occurs, and can help the cow to supplement qi and consolidate the exterior, support the healthy and eliminate the evil, improve the immunity, and then increase the weight of the calf, improve the anemia of the calf, reduce the inflammatory response, improve the liver and kidney functions of the calf, and regulate and enhance the immunity while ensuring the feeding quality. DETAILED DESCRIPTION

[0019] The application will be further described in detail below in combination with examples, but the protection scope of the application is not limited to the following examples.

[0020] Example 1

[0021] A traditional Chinese medicine composition for preventing and treating calf diarrhea is prepared from the following weight parts of traditional Chinese medicine raw materials: Radix Bupleuri 5 parts, Paeonia 4 parts, Fructus Aurantii Immaturus 5 parts, Alisma 3 parts, Atractylodes 5 parts, Astragalus 5 parts, Scutellaria 4 parts, Saposhnikovia 4 parts, Poria 3 parts, Tangerine Peel 3 parts, Gardenia 4 parts, and Licorice 3 parts.

[0022] Example 2

[0023] A traditional Chinese medicine composition for preventing and treating calf diarrhea is prepared from the following weight parts of traditional Chinese medicine raw materials: Radix Bupleuri 5 parts, Paeonia 3 parts, Fructus Aurantii Immaturus 4 parts, Alisma 4 parts, Atractylodes 6 parts, Astragalus 5 parts, Scutellaria 5 parts, Saposhnikovia 4 parts, Poria 4 parts, Tangerine Peel 3 parts, Gardenia 4 parts, and Licorice 3 parts.

[0024] Example 3

[0025] A traditional Chinese medicine composition for preventing and treating calf diarrhea is prepared from the following weight parts of traditional Chinese medicine raw materials: Radix Bupleuri 6 parts, Paeonia 3 parts, Fructus Aurantii Immaturus 4 parts, Alisma 5 parts, Atractylodes 5 parts, Astragalus 5 parts, Scutellaria 4 parts, Saposhnikovia 4 parts, Poria 3 parts, Tangerine Peel 4 parts, Gardenia 4 parts, and Licorice 4 parts.

[0026] Example 4

[0027] A traditional Chinese medicine composition for preventing and treating calf diarrhea is prepared from the following weight parts of traditional Chinese medicine raw materials: Radix Bupleuri 5 parts, Paeonia 4 parts, Fructus Aurantii Immaturus 4 parts, Alisma 3 parts, Atractylodes 6 parts, Astragalus 6 parts, Scutellaria 4 parts, Saposhnikovia 5 parts, Poria 4 parts, Tangerine Peel 4 parts, Gardenia 5 parts, and Licorice 4 parts.

[0028] The four examples above can be made into powder or granular preparations. Radix Bupleuri, Paeonia, Fructus Aurantii Immaturus, Alisma, Atractylodes, Astragalus, Scutellaria, Saposhnikovia, Poria, Tangerine Peel, Gardenia and Licorice are dried, crushed, and sieved through a 100-180 mesh screen in sequence, and then mixed uniformly according to the above weight parts to obtain a traditional Chinese medicine powder. The traditional Chinese medicine powder is mixed into forage for feeding or fed by pouring warm water, and the dosage is generally 40-80 g per head, fed once a day, and continuously fed for 4-7 days, so that a satisfactory effect can be obtained.

[0029] The pharmacological effects of each Chinese medicine raw material in the Chinese medicine composition of the present application are as follows:

[0030] Radix Bupleuri has the effects of relieving fever, soothing the liver and relieving depression, and lifting Yang Qi. When combined with Radix Scophulariae, Radix Bupleuri lifts clear and relieves depression, and Radix Scophulariae reduces turbid and purges fire, together playing the roles of lifting clear and reducing turbid, relieving depression and fever, and harmonizing Shaoyang. When combined with Radix Paeoniae Alba, Radix Bupleuri and Radix Paeoniae Alba play the roles of lifting and reducing, restraining and using each other, making Qi unobstructed, blood unobstructed, clear Qi ascending, and turbid Qi descending, together playing the roles of soothing the liver, regulating Qi, dispersing the stomach, regulating Qi, and resolving masses. Radix Bupleuri is light, clear, pungent, and dispersing, which can lead clear Yang Qi to ascend from the left to regulate Shaoyang Qi, regulate the liver and spleen, regulate the middle palace, and resolve distention and fullness. Radix Paeoniae Alba is sour, cold, and astringent, which can astringe body fluid to protect blood, astringe Yang Qi to purge pathogenic heat, nourish blood to soften the liver, relieve pain, and purge the liver of pathogenic heat to supplement spleen Yin.

[0031] Radix Scophulariae and Radix Scophulariae are combined, and the two drugs are used together to reduce and purge, and treat Qi and blood together. Together, they play the roles of clearing heat and purging fire, drying dampness and relieving restlessness, and cooling blood and stopping bleeding. Radix Astragali, Radix Atractylodis Macrocephalae, and Radix Saposhnikoviae are taken from Jade Screen Powder, which is used to treat body weakness caused by long-term diarrhea in calves, which is easy to cause external pathogens to enter. Jade Screen Powder supplements the spleen and strengthens the exterior, supports the healthy and expels pathogenic factors, and treats the root and branch. Radix Poriae, Rhizoma Alpiniae Oxophyllae, and Pericarpium Citri Reticulatae can remove spleen dampness and invigorate the spleen, and can enhance the effect of stopping diarrhea. Radix Glycyrrhizae can prevent bitter and cold drugs from damaging the stomach, and can harmonize various drugs, which is the king of drugs.

[0032] Veterinary dialectical of calf diarrhea is liver and gallbladder heat and liver and spleen disharmony. Combined with the symptoms and pathological changes of the disease, it is necessary to supplement Qi, consolidate the exterior, support the healthy, expel pathogenic factors, and improve immunity.

[0033] The clinical effect of the present application is tested and verified as follows:

[0034] 30 calves of about 10 days old with diarrhea were randomly selected from a certain dairy farm and randomly divided into a test group and a control group, 15 calves in each group.

[0035] 1 Materials and methods

[0036] 1.1 Test medicinal materials

[0037] The Chinese medicine composition of Example 1, Radix Bupleuri, Paeoniae, Aurantii, Alpiniae Oxophyllae, Atractylodis Macrocephalae, Scophulariae, Saposhnikoviae, Poriae, Citri Reticulatae, and Glycyrrhizae were mixed according to the mass ratio of 5:4:5:3:5:5:4:4:3:3:4:3, and the drugs were crushed to 180 mesh powder.

[0038] 1.2 Reagents and equipment

[0039] Immunological marker detection kits were purchased from Beijing Kemei Dongya Biotechnology Co., Ltd.; superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) detection kits were purchased from Nanjing Jiancheng Biotechnology Co., Ltd.; traditional Chinese medicine was purchased from Weifang Yibusheng Pharmaceutical Co., Ltd.; ceftiofur sodium was purchased from Qilu Animal Health Products Co., Ltd. IDEXX fully automated hematology analyzer (model number not provided) The following instruments were used: a Roche fully automated biochemical analyzer (model cobasc311); a Molecular Devices ELISA reader (model SpectraMax i3x); and 10mL syringes, anticoagulant tubes, and coagulation-promoting tubes, which were stored in the bovine and ovine disease prevention and control laboratory.

[0040] 1.3 Grouping and Treatment Methods for Experimental Animals

[0041] The experimental group received treatment with a traditional Chinese medicine composition. Starting on the first day, researchers administered 60g of the composition to 100mL of physiological saline via a bovine dosing bottle, continuing this treatment for 7 days. The control group received injections of ceftiofur sodium and were administered an equal volume of physiological saline via the same method. The therapeutic effects of the two groups were compared. During the experiment, the feeding management, relative humidity, and lighting conditions were identical for both groups.

[0042] 1.4 Measurement Indicators and Methods

[0043] (1) On days 1 and 7 of the experiment, 5 mL of blood was collected from the jugular vein of each calf. 2 mL was stored in an EDTA anticoagulant tube for routine blood tests, and the remaining 3 mL was added to a procoagulant tube, allowed to stand, and the supernatant serum was collected and stored at -20℃ for later use. Blood biochemistry, antioxidant indicators, and immune factors were measured in the serum samples according to the instructions of the corresponding instruments and reagent kits. The recovery status of calf diarrhea was recorded during the experiment.

[0044] (2) On day 1 and day 7 of the experiment, the weight of all participating cattle was measured and the average daily weight gain was calculated.

[0045] 1.5 Assessment of Diarrhea in Calves

[0046] Observe and score the feces of the participating calves daily: 1 point for feces that are strip-shaped or granular; 2 points for soft, well-formed feces; 3 points for thick, unformed feces without separation of feces and water; and 4 points for liquid, unformed feces with separation of feces and water. A feces score of 2 or below is considered cured.

[0047] 2. Data Statistics and Analysis

[0048] The original data were arranged by Excel, the data were statistically analyzed by SPSS25.0 statistical software, the single factor variance analysis was conducted, the independent sample t test was used for difference analysis between groups. The results were expressed as "mean ± standard error", P<0.01 represented extremely significant difference, P<0.05 represented significant difference, and P>0.05 represented no significant difference.

[0049] 3. Results

[0050] 3.1 Treatment results

[0051] The treatment results of diarrhea calves in the test group were 14 heads of cured calves, and the cure rate was 93.3%; the treatment results of diarrhea calves in the control group were 12 heads of cured calves, and the cure rate was 80%; the cure rate of the test group was significantly different from that of the control group (P<0.05). The average cure days of the test group were 3.53d, and the average cure days of the control group were 4.33d, and the average cure days of the test group were significantly different from those of the control group (P<0.05).

[0052] Table 1 Treatment results of diarrhea calves.

[0053]

[0054] 3.2 Changes in body weight of test calves

[0055] The changes in body weight of calves during the test period were shown in Table 2. The results showed that the average initial body weight of calves in the test group was 43.98kg, and the average initial body weight of calves in the control group was 44.03kg, and the difference was not significant (P>0.05). After treatment, the average body weight of calves in the test group was 50.91kg, and the average body weight of calves in the control group was 50.11kg, and the difference was significant (P<0.05). Among them, the average daily weight gain of calves in the test group was 0.99kg, and the average daily weight gain of calves in the control group was 0.87kg, and the difference was significant (P<0.05).

[0056] Table 2 Changes in body weight of diarrhea calves during the test period.

[0057] Item Test group Control group P value Average body weight on test day 1 (kg) 43.98 44.03 P>0.05 Average body weight on test day 7 (kg) 50.91 50.11 P<0.05 Average daily weight gain (kg) 0.99 0.87 P<0.05

[0058] The reason why the Chinese herbal composition of the test group improved the growth performance of calves may be that Chinese herbal medicine has the effects of benefiting qi, consolidating the exterior, strengthening the body resistance, invigorating the spleen, etc., which can achieve the effects of stopping diarrhea, improving the appetite of calves, promoting the digestion of calves, and thus improving the growth performance of calves.

[0059] 3.3 Effects of Chinese herbal composition on blood routine

[0060] The results of blood routine test are shown in Table 3. The results show that the average volume of red blood cells of the test group is significantly increased (P<0.01) before and after the test, the average hemoglobin concentration and the average hemoglobin amount of red blood cells are significantly increased (P<0.05), and the number of red blood cells is not statistically different, which shows that the anemia of the test group is effectively improved.

[0061] The number of white blood cells of the control group is significantly decreased (P<0.05) before and after the test, which shows that the level of white blood cells is decreased after the use of antibiotics.

[0062] Table 3. The results of blood routine test.

[0063]

[0064] 3.4 Influence of the Chinese medicine composition on blood biochemistry, immunity and antioxidant factors

[0065] The results of blood biochemistry are shown in Table 4. The results show that the total bilirubin of the test group is extremely significantly different (P<0.01) before and after the test, the alkaline phosphatase, γ-glutamyltransferase, total protein and urea of the test group are significantly different (P<0.05) before and after the test, and only the total bilirubin of the control group is significantly different (P<0.05) before and after the test.

[0066] Table 4. The results of blood biochemistry test.

[0067]

[0068] The results of immunity and antioxidant factors are shown in Table 5. The results show that each index in the table is changed before and after the test of the test group, the IgG, IgM and IgA of the test group are extremely significantly different (P<0.01) before and after the test, and the T-AOC and SOD of the test group are significantly different (P<0.05) before and after the test. The IgA of the control group is extremely significantly different (P<0.01) before and after the test, and the IgM of the control group is significantly different (P<0.05) before and after the test, which shows that the use of the Chinese medicine composition can improve the immunity of the calf and the effect of enhancing the antioxidant capacity of the calf is better than that of the antibiotic.

[0069] Table 5. The results of immunity and antioxidant factor test.

[0070]

[0071] According to Table 4 and Table 5, the serum total protein, immunity index and antioxidant index of the test group are obviously increased before and after the test, and the urea and alkaline phosphatase are obviously decreased.

[0072] The content of serum total protein reflects the strength of protein synthesis and metabolism in vivo. When the content of serum total protein is increased, it is beneficial to improve the metabolism level and immunity and promote the healthy growth of animals.

[0073] The content of urea represents the absorption and metabolism of protein in the animal body, and the content of urea nitrogen in serum will decrease when the body has good protein metabolism.

[0074] Alkaline phosphatase is a lysosomal enzyme in the phagocyte of the calf body, and has an important role in the phagocytic ability and bactericidal ability of the phagocyte of the calf. The activity of alkaline phosphatase reflects the growth rate and growth performance of the calf, and is a commonly used index for diagnosing diseases of the biliary system. The increase of alkaline phosphatase indicates obstructive lesions of the biliary tract, cholestatic hepatitis and extrahepatic obstruction, and fibrosis of the liver. The significant decrease of the index of alkaline phosphatase in the test group indicates that the damage caused by diarrhea has been repaired.

[0075] The antioxidant defense system is a complete and complex defense system formed in the evolution of the animal body, and T-AOC is the main system for antagonizing oxygen free radicals in the body and is a comprehensive index for measuring the antioxidant capacity of the body. The index reflects the resistance of the whole enzymatic and non-enzymatic antioxidant system to oxidative stress.

[0076] In summary, the traditional Chinese medicine composition provided by the present application has no adverse effect on the protein metabolism, lipid metabolism and liver function of the calf with diarrhea, and the traditional Chinese medicine prescription can improve the cure rate of diarrhea in the calf, help the calf to gain weight, improve anemia in the calf, and improve the immunity and antioxidant capacity of the calf.

Claims

1. A traditional Chinese medicine composition for preventing and treating calf diarrhea, characterized in that, the composition comprises the following components: The Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Bupleurum 5-10 parts, Paeonia 1-5 parts, Citrus aurantium 1-5 parts, Alisma 1-5 parts, Atractylodes 5-10 parts, Astragalus 5-10 parts, Scutellaria 1-5 parts, Saposhnikovia 1-5 parts, Poria 1-5 parts, Citrus reticulata 1-5 parts, Gardenia 1-5 parts, and Glycyrrhiza 1-5 parts. ​ 2. The traditional Chinese medicine composition for controlling calf diarrhea according to claim 1, characterized in that, The Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Bupleurum 5 parts, Paeonia 4 parts, Citrus aurantium 5 parts, Alisma 3 parts, Atractylodes 5 parts, Astragalus 5 parts, Scutellaria 4 parts, Saposhnikovia 4 parts, Poria 3 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

3. The Chinese medicine composition for preventing and treating calf diarrhea according to claim 1, characterized in that, The Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Bupleurum 5 parts, Paeonia 3 parts, Citrus aurantium 4 parts, Alisma 4 parts, Atractylodes 6 parts, Astragalus 5 parts, Scutellaria 5 parts, Saposhnikovia 4 parts, Poria 4 parts, Citrus reticulata 3 parts, Gardenia 4 parts, and Glycyrrhiza 3 parts.

4. The Chinese medicine composition for preventing and treating calf diarrhea according to claim 1, characterized in that, The Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Bupleurum 6 parts, Paeonia 3 parts, Citrus aurantium 4 parts, Alisma 5 parts, Atractylodes 5 parts, Astragalus 5 parts, Scutellaria 4 parts, Saposhnikovia 4 parts, Poria 3 parts, Citrus reticulata 4 parts, Gardenia 4 parts, and Glycyrrhiza 4 parts.

5. The Chinese medicine composition for preventing and treating calf diarrhea according to claim 1, characterized in that, The Chinese medicine composition is prepared from the following Chinese medicine raw materials by weight: Bupleurum 5 parts, Paeonia 4 parts, Citrus aurantium 4 parts, Alisma 3 parts, Atractylodes 6 parts, Astragalus 6 parts, Scutellaria 4 parts, Saposhnikovia 5 parts, Poria 4 parts, Citrus reticulata 4 parts, Gardenia 5 parts, and Glycyrrhiza 4 parts.

6. The Chinese medicine composition for preventing and treating calf diarrhea according to claim 5, characterized in that, The dosage form of the Chinese medicine composition is powder or granular preparation.

7. A method for preparing the traditional Chinese medicine composition according to any one of claims 1-6, characterized in that, The method comprises the following steps: Each Chinese medicine raw material is dried, crushed and sieved in sequence, and then mixed uniformly by weight to obtain the Chinese medicine composition.

8. The preparation method of the traditional Chinese medicine composition according to claim 7, characterized in that, Each Chinese medicine raw material is taken by weight, and decoction-free granular preparation is prepared by using supercritical extraction or Chinese medicine decoction process.

9. Use of the Chinese medicine composition of any one of claims 1-6 or the Chinese medicine composition prepared by the preparation method of any one of claims 7-8 in the preparation of a medicine for preventing and treating calf diarrhea.

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