A method for cultivating a specialized strain of Wenchang chicken that replaces resistance

Abstract

The present invention discloses a method for cultivating a specialized strain of Wenchang chicken that replaces antibiotics. The method utilizes a net-based flat-bed method for breeding during the brooding period; the brooding feed includes corn flour, wheat bran, soybean meal, lard, fresh sweet potato, fermentation residue, fish meal, and Hainan tiger nanmu; during the brooding process, fermentation liquid is added to the drinking water; and during the rearing period, the chickens are raised in cages and fed with cage feed; the cage feed ratio includes corn flour, fresh sweet potato, wheat bran, rice, soybean meal, rice bran, southern dew grass, dandelion, and lard. The present invention effectively combines microbial technology with traditional Chinese medicine to form a method for breeding a specialized strain of Wenchang chicken that replaces antibiotics. This method can replace the use of antibiotics, greatly improve the survival rate of Wenchang chickens, promote the growth and reproduction of Wenchang chickens, and improve muscle quality, thereby increasing the commercial value of Wenchang chickens.

Description

Technical Field

[0001] The invention belongs to the technical field of Wenchang chicken breeding, and in particular relates to a method for breeding a specialized strain of Wenchang chicken that replaces resistance. Background Art

[0002] Wenchang chicken, native to Wenchang City, Hainan Province, is a unique avian genetic resource of Hainan Province and the only local chicken breed recognized by the National Animal Genetic Resources Appraisal Committee. Adult Wenchang chickens are compact, with plumage that is typically reddish-brown (male) and tawny (female), short, slender legs, and thin, crispy skin and bones. Wenchang chicken is renowned for its sweet, tender meat, rich flavor, color, aroma, and nutritional value. As a unique landmark of Hainan's poultry industry, the overall scale and output value of Wenchang chickens have been increasing in recent years, making them a key driver of Hainan's livestock and poultry development.

[0003] Antibiotics can prevent disease and promote growth, playing a vital role in the livestock and poultry industries. However, their use as feed additives also presents challenges, such as impacts on human health. Furthermore, the long-term and extensive use of antibiotics can lead to the development of antibiotic-resistant bacteria. Therefore, promoting antibiotic-free farming is a growing trend in the livestock and poultry industries. Currently, reports on alternative methods for raising Wenchang chickens using antibiotics are relatively rare. Improving the muscle quality of Wenchang chickens while using these alternatives, and thus further enhancing their commercial value, is a key area of ​​focus. Summary of the Invention

[0004] In view of the deficiencies in the prior art, the present invention provides a method for breeding a specialized strain of Wenchang chicken that replaces the resistance.

[0005] The technical solutions of the present invention are as follows:

[0006] A method for cultivating a specialized strain of Wenchang chicken that replaces resistance to antibiotics comprises the following steps:

[0007] (1) Breeding during the brooding period:

[0008] The fish are raised in a flat-surface manner on the net;

[0009] The brooding feed ratio is: corn flour 75-65g, wheat bran 22-30g, soybean meal 25-30g, fermentation residue 20-30g, fresh sweet potato 15-17g, lard 10-15g, fish meal 10-12g, Hainan tiger skin 8-11g, water 3-13g;

[0010] The fermentation residue is prepared by fully mixing the bacterial liquid with the fermentation raw materials and then fermenting for 3-5 days, filtering to obtain the fermentation liquid and the fermentation residue; during the brooding process, the fermentation liquid is added to the drinking water; and the fermentation residue is added to the brooding feed;

[0011] (2) Cage breeding is used during the rearing period, and cage feed is fed;

[0012] The cage feed ratio is: 50~72g corn flour, 20~30g fresh sweet potato, 20~30g southern dew grass, 20~30g wheat bran, 10~18g rice, 12~20g rice bran, 10~20g soybean meal, 8~10g dandelion, 3~6g lard, and 1~14g water.

[0013] Preferably, the brooding period is 1-21 days of age for Wenchang chickens.

[0014] Preferably, the ratio of the brooding feed is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger skin nan, and 13g water.

[0015] Preferably, the fermentation raw material is Buddleja buddhistata.

[0016] Preferably, the bacterial solution is a Bacillus splendens bacterial solution, the inoculation mass of the bacterial solution is 60-80w / w%, and the bacterial content of the bacterial solution is 10 7 ~10 8 cfu / mL.

[0017] Preferably, the breeding density during the brooding period is 30-40 birds / m 2 , light intensity is 30±5 lx; the density during the rearing period shall not exceed 20 / m 2 , daily lighting time is 9-10 h, and light intensity is 50±5 lx.

[0018] Preferably, the cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, and water 10g.

[0019] Preferably, the amount of fermentation liquid added to drinking water is 3-5w / w%.

[0020] Preferably, the Wenchang chicken is a healthy Wenchang chicken with silvery-white feathers and strong growth as the male parent; a healthy Wenchang chicken with golden feathers and strong growth as the female parent, and the offspring Wenchang chicken is obtained by hybridization.

[0021] Preferably, the fermentation residue is prepared by inoculating bacterial liquid into fermentation raw materials, stirring the mixture thoroughly, and then standing the mixture for fermentation. During the fermentation process, the mixture is stirred every 1 to 1.5 hours. After fermentation for 3 to 5 days, the mixture is filtered to obtain the fermentation liquid and the fermentation residue.

[0022] Beneficial effects of the present invention:

[0023] This invention combines microbial fermentation technology with traditional Chinese medicine techniques to develop a specialized strain of Wenchang chicken that replaces antibiotics. This method significantly improves the survival rate of Wenchang chickens, reaching over 95% without the use of antibiotics. The breeding process effectively enhances the chicken's functional abilities, promotes its growth and reproduction, and improves meat quality, thereby increasing its commercial value. DETAILED DESCRIPTION

[0024] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0025] In this study, the silver-white feather trait of the Wenchang chicken is specifically that the neck and back of the rooster are mainly white, but with black or yellow spots, the abdomen and wings are yellow, and the tail is black; the hen is black, with white lace on the neck feathers.

[0026] In this study, the golden feather trait specifically refers to the rooster's golden feather trait: the neck and back are golden yellow, while the abdomen, tail and wings are black; the hen's neck and abdomen feathers are black with golden lace, and the rest of the feathers are black.

[0027] Example 1: Method for breeding a specialized strain of Wenchang chicken with alternative resistance

[0028] (1) Select healthy Wenchang chickens with silvery-white feathers and strong growth as the male parent; select healthy Wenchang chickens with golden feathers and strong growth as the female parent, and hybridize them to obtain offspring Wenchang chickens;

[0029] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0030] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0031] The brooding feed mix is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0032] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0033] (3) Breeding during the growing period:

[0034] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0035] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0036] Example 2: Method for Breeding a Specialized Strain of Antibiotic-Resistant Wenchang Chicken

[0037] (1) Select healthy Wenchang chickens with silvery-white feathers and strong growth as the male parent; select healthy Wenchang chickens with golden feathers and strong growth as the female parent, and hybridize them to obtain offspring Wenchang chickens;

[0038] (2) During the brooding period (1-21 days), the chicks are raised on the net with a density of 30 per m 2 , light intensity is 30±5lx.

[0039] The brooding feed ratio is: 65g corn flour, 30g wheat bran, 30g fermentation residue, 25g soybean meal, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 3g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0040] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja ) at 60 w / w% inoculum mass. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% fermentation liquid was added to the drinking water; the fermentation residue was added to the chick rearing feed.

[0041] (3) Breeding during the growing period:

[0042] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 The optimal light duration is 9 hours per day, the light intensity is 50±5 lx, and the chickens are fed with cage feed; the chickens are fed three times a day, and the daily feeding amount is 6%-8% of their body weight;

[0043] The cage feed ratio is: corn flour 72g, southern dew grass 30g, fresh sweet potato 25g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 1g. Grind and mix all the feed ingredients to obtain the feed.

[0044] Example 3: Cultivation Method for a Specialized Anti-Resistant Wenchang Chicken Strain

[0045] (1) Select healthy Wenchang chickens with silvery white feathers and strong growth as the male and female parents, and hybridize them to obtain the offspring Wenchang chickens;

[0046] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0047] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0048] The brooding feed mix is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0049] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0050] (3) Breeding during the growing period:

[0051] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0052] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0053] Example 4: Cultivation Method of Specialized Antibiotic Wenchang Chicken Strain

[0054] (1) Select healthy Wenchang chickens with golden feathers and strong growth as parents, and hybridize them to obtain offspring Wenchang chickens;

[0055] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0056] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0057] The brooding feed mix is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0058] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0059] (3) Breeding during the growing period:

[0060] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0061] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0062] Example 5: Cultivation Method of Specialized Antibiotic-Resistant Wenchang Chicken Strain

[0063] (1) Select healthy Wenchang chickens with silvery-white feathers and strong growth as the male parent; select healthy Wenchang chickens with golden feathers and strong growth as the female parent, and hybridize them to obtain offspring Wenchang chickens;

[0064] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0065] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0066] The brooding feed mix is: 75g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 15g fresh sweet potato, 10g lard, 10g fish meal, 8g Hainan tiger nut, and 7g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0067] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 8 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0068] (3) Breeding during the growing period:

[0069] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0070] The cage feed ratio is: 65g corn flour, 20g fresh sweet potato, 20g southern dew grass, 30g wheat bran, 18g rice, 12g rice bran, 20g soybean meal, 8g dandelion, 3g lard, and 4g water. Grind and mix the feed ingredients to obtain the feed.

[0071] Example 6: Breeding Method for a Specialized Strain of Antibiotic-Resistant Wenchang Chicken

[0072] (1) Select healthy Wenchang chickens with silvery-white feathers and strong growth as the male parent; select healthy Wenchang chickens with golden feathers and strong growth as the female parent, and hybridize them to obtain offspring Wenchang chickens;

[0073] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0074] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0075] The brooding feed mix is: 65g corn flour, 30g soybean meal, 22g wheat bran, 20g fermentation residue, 15g fresh sweet potato, 15g lard, 12g fish meal, 11g Hainan tiger nan, and 10g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0076] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja ) at an inoculum rate of 80 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every 1.5 hours during the fermentation process. After 5 days of fermentation, the fermentation liquid and fermentation residue were filtered. During the brooding process, 5 w / w% fermentation liquid was added to the drinking water, and the fermentation residue was added to the brooding feed.

[0077] (3) Breeding during the growing period:

[0078] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0079] The cage feed ratio is: 50g corn flour, 30g fresh sweet potato, 30g southern dew grass, 20g wheat bran, 10g rice, 20g rice bran, 10g soybean meal, 10g dandelion, 6g lard, and 14g water. Crush and mix all the feed ingredients to obtain the feed.

[0080] Comparative Example 1: Breeding method for a specialized strain of Wenchang chicken with alternative resistance

[0081] (1) Select healthy Wenchang chickens with golden feathers and strong growth as parents, and hybridize them to obtain offspring Wenchang chickens;

[0082] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0083] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0084] The brooding feed mix is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0085] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Purslane) at a rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0086] (3) Breeding during the growing period:

[0087] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0088] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0089] Comparative Example 2: Breeding method for a specialized strain of Wenchang chicken with alternative resistance

[0090] (1) Select healthy Wenchang chickens with golden feathers and strong growth as parents, and hybridize them to obtain offspring Wenchang chickens;

[0091] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0092] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0093] The brooding feed mix is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger nan, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0094] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0095] (3) Breeding during the growing period:

[0096] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0097] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, purslane 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0098] Comparative Example 3: Breeding method for a specialized strain of Wenchang chicken with alternative resistance

[0099] (1) Select healthy Wenchang chickens with golden feathers and strong growth as parents, and hybridize them to obtain offspring Wenchang chickens;

[0100] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0101] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0102] The brooding feed ratio is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g purslane, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0103] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Buddha Buddleja), at an inoculum rate of 60 w / w%. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the chick rearing process, 3 w / w% of the fermentation liquid was added to the drinking water, and the fermentation residue was added to the chick rearing feed.

[0104] (3) Breeding during the growing period:

[0105] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0106] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, southern dew grass 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0107] Comparative Example 4-Wenchang chicken breeding method with added antibiotics

[0108] (1) Select healthy Wenchang chickens with golden feathers and strong growth as parents, and hybridize them to obtain offspring Wenchang chickens;

[0109] (2) Breeding of offspring Wenchang chickens during the brooding period (1-21 days):

[0110] The fish are raised online with a density of 30 per m 2 , light intensity is 30±5 lx.

[0111] The brooding feed ratio is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g purslane, and 13g water. Grind and mix all ingredients to make the feed. Feed three times daily at a daily feed rate of 6%-8% of the chicken's body weight.

[0112] The fermentation residue is: the bacterial solution of Paenibacillus splendidus (commercially available strain, CGMCC1.634) (the bacterial solution concentration is 10 7 cfu / mL) was inoculated into the fermentation raw material (Purslane) at 60 w / w% inoculum mass. After thorough stirring, the mixture was allowed to ferment. Stir regularly every hour during the fermentation process. After three days of fermentation, the fermentation liquid and fermentation residue were filtered. During the brooding process, 3 w / w% fermentation liquid was added to the drinking water, and the fermentation residue was added to the brooding feed.

[0113] (3) Breeding during the growing period:

[0114] During the rearing period, the animals are raised in cages with a density not exceeding 20 per cubic meter. 2 , light duration 9 h per day, light intensity 50±5 lx, feeding cage feed; feeding three times a day, daily feeding amount is 6%-8% of chicken body weight;

[0115] The cage feed ratio is: corn flour 72g, fresh sweet potato 25g, purslane 21g, wheat bran 20g, rice 13g, rice bran 14g, soybean meal 12g, dandelion 9g, lard 4g, water 10g. Grind and mix the feed ingredients to obtain the feed.

[0116] During the brooding period, add erythromycin 5g / 10kg water to drinking water for three consecutive days. During the rearing stage, add oxytetracycline 2w / w% to drinking water for three consecutive days.

[0117] In Examples 1-4 and Comparative Examples 1-4 above, conventional sterilization and disinfection methods were used to reduce the risk of chickens contracting diseases. During the breeding process of Examples 1-4 and Comparative Examples 1-3 above, feed and its raw materials, drinking water, etc. were tested to ensure that no antibiotics were used during the breeding process.

[0118] Experimental example

[0119] Test indicators and measurement methods

[0120] 1. Experimental Animals

[0121] Each group of Wenchang chicken hens.

[0122] 2. Production performance indicators

[0123] The weight at 1 day of age, average slaughter weight at 115 days of age, and survival rate were recorded.

[0124] 3. Meat quality and general nutritional indicators

[0125] Wenchang chicken breast muscle samples were taken to measure meat quality indicators such as pH, shear force, and drip loss. The meat quality calculation method is as follows:

[0126] Shear force: Take a 3 cm long, 1 cm wide, and 0.5 cm thick chest muscle sample, remove the fascia, and measure the shear force value using a muscle tenderizer. When shearing, the knife should be perpendicular to the direction of the muscle fibers of the meat sample. Shear each sample three times and calculate the average value.

[0127] Drip loss: Take a piece of meat sample of appropriate weight and weigh it accurately (M1); fix one end with wire so that the muscle fibers naturally point vertically downward, and hang it in a plastic bag (keeping the meat sample from touching the plastic bag). Tie the bag tightly and hang it in the refrigerator at 4°C for 24 hours; remove the meat sample, wipe the surface moisture with absorbent paper, and weigh it again (M2). Calculate drip loss = (M1-M2) / M1*100%

[0128] pH: Take fresh breast muscle after slaughter and use a carcass muscle pH meter to directly insert it into the muscle for measurement.

[0129] Table 1 Production performance of Wenchang chicken

[0130]

[0131] As can be seen from Table 1, the average market weight of the Wenchang chickens in the embodiment is significantly higher than that in the control group; the survival rate of the Wenchang chickens in the embodiment group is higher than that in the control group. Compared with Example 3 and Example 4, Example 1 and Example 2 hybridize yellow feathers and white feathers, and the survival rate and market weight of the offspring are higher, indicating that the offspring of the yellow feather and white feather hybrid have higher disease resistance and growth performance. Compared with Example 4 and other control examples, the market weight and survival rate of Control Example 1 are significantly lower, indicating that the fermented product of Buddleja buddleja has a significant effect on the disease resistance and growth performance of Wenchang chicken. The results of Control Examples 2 and 3 also show that southern dewdrop grass and Hainan tiger skin nan are beneficial to improving the survival rate and growth performance. The overall results show that the breeding method of the embodiment is suitable for the cultivation of disease-resistant strains of Wenchang chicken and can replace the use of antibiotics.

[0132] The effects on the quality of Wenchang chicken are shown in Table 2.

[0133] Table 2 Effects of different breeding modes on the quality of Wenchang chicken

[0134]

[0135] As shown in Table 2, the drip loss and shear force of the embodiment group are lower than those of the comparative group, indicating that the meat quality of the embodiment group is better; the differences in pH index are not significant ( P> 0.05).

[0136] The above descriptions are only some embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A method for breeding a specialized strain of Wenchang chicken, characterized in that: The following steps are involved: (1) Breeding during the brooding period: The fish are raised in a flat-surface manner on the net; The brooding feed ratio is: corn flour 75-65g, wheat bran 22-30g, soybean meal 25-30g, fermentation residue 20-30g, fresh sweet potato 15-17g, lard 10-15g, fish meal 10-12g, Hainan tiger skin 8-11g, water 3-13g; The fermentation residue is obtained by fully mixing the bacterial liquid and the fermentation raw materials, fermenting them for 3-5 days, and filtering to obtain the fermentation liquid and the fermentation residue; during the brooding process, the fermentation liquid is added to the drinking water; the fermentation residue is added to the brooding feed; the bacterial liquid is the bacterial liquid of Paenibacillus splendidus; and the fermentation raw material is Buddleja buddleja. (2) During the rearing period, the fish are raised in cages and fed with cage feed; The cage feed ratio is: 50~72g corn flour, 20~30g fresh sweet potato, 20~30g southern dew grass, 20~30g wheat bran, 10~18g rice, 12~20g rice bran, 10~20g soybean meal, 8~10g dandelion, 3~6g lard, and 1~14g water.

2. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The brooding period is 1-21 days old for Wenchang chickens.

3. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The ratio of the brooding feed is: 65g corn flour, 30g wheat bran, 25g soybean meal, 20g fermentation residue, 17g fresh sweet potato, 12g lard, 10g fish meal, 8g Hainan tiger skin nan, and 13g water.

4. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The bacterial solution is a bacillus splendens bacterial solution, the inoculation mass of the bacterial solution is 60-80w / w%, and the bacterial content of the bacterial solution is 10 7 ~10 8 cfu / mL.

5. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The breeding density during the brooding period is 30-40 birds / m 2 , light intensity is 30±5 lx; the density during the rearing period shall not exceed 20 / m 2 , daily lighting time is 9-10 h, and light intensity is 50±5 lx.

6. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The cage feed ratio is: 72g corn flour, 25g fresh sweet potato, 21g southern dew grass, 20g wheat bran, 13g rice, 14g rice bran, 12g soybean meal, 9g dandelion, 4g lard, and 10g water.

7. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The amount of fermentation liquid added to drinking water is 3~5w / w%.

8. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The Wenchang chicken is a healthy Wenchang chicken with silvery-white feathers and strong growth as the male parent; a healthy Wenchang chicken with golden feathers and strong growth as the female parent, and the offspring Wenchang chicken is obtained by hybridization.

9. The method for breeding a specialized strain of Wenchang chicken according to claim 1, characterized in that: The fermentation residue is prepared by inoculating bacterial liquid into fermentation raw materials, stirring the mixture thoroughly, and then allowing the mixture to ferment. During the fermentation process, the mixture is stirred every 1 to 1.5 hours. After fermentation for 3 to 5 days, the mixture is filtered to obtain the fermentation liquid and fermentation residue.

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