Detection of skeletal muscle atrophy markers in pigs infected with Haemophilus parasuis

Abstract

The present invention relates to a marker for detecting the skeletal muscle atrophy of pigs infected with Haemophilus parasuis, wherein the marker at least includes FOXO1, FBXO32, TRIM63, CTSL and BNIP3; when the expression levels of FOXO1, FBXO32, TRIM63, CTSL and BNIP3 are higher than the average expression levels of healthy individuals, muscle fiber atrophy occurs in pork. The present invention compares the expression levels of the markers of individuals infected with Haemophilus parasuis with the average expression levels of healthy individuals; according to the comparison results, the skeletal muscle atrophy of the individuals infected with Haemophilus parasuis is determined, and then the meat production trait is determined according to the skeletal muscle atrophy, and the method can also be used to screen out individuals with non-atrophic skeletal muscles for breeding.

Description

Technical Field

[0001] The invention relates to the fields of biotechnology and animal husbandry, and in particular to a marker for detecting the skeletal muscle atrophy of pigs infected with Haemophilus parasuis and an application thereof. Background Art

[0002] As an important meat source for humans, pork provides high-quality protein and rich nutrients. Skeletal muscle is the largest tissue in the pig's body weight, and the meat production-related traits of pigs are mainly determined by the growth and development of skeletal muscle. The skeletal muscle development process includes the determination of the number of muscle fibers in the embryonic period, the conversion of muscle fiber types before and after birth, and the hypertrophy and maturation of muscle fibers. During postnatal development, the increase in skeletal muscle mass mainly depends on the hypertrophy process of muscle fibers. Therefore, postnatal muscle fiber development directly affects the state of pig muscle tissue. Detecting and evaluating the growth and development status of skeletal muscle and ensuring normal development of skeletal muscle are crucial to improving pork production and pig farming efficiency. Muscle atrophy occurs when protein degradation in muscle fibers increases and protein synthesis decreases. It is particularly easy to induce under starvation and disease conditions, and has a negative impact on pig muscle quality and yield.

[0003] Haemophilus parasuis (G. parasuis) is a Gram-negative bacterium that mainly parasitizes the respiratory tract of pigs and may further invade the blood circulation to cause Glaser's disease. The disease has multiple pathological characteristics, including various sepsis, arthritis, pleurisy and meningitis. Haemophilus parasuis is a highly infectious bacterium in pig herds and often exists in asymptomatic infections. This bacterial infection often causes muscle atrophy in pigs, which directly leads to a decrease in muscle weight, thereby reducing pork production and causing serious damage to the pig industry. In the prior art, muscle health and function are evaluated through multiple indicators such as muscle mass measurement, muscle strength testing, muscle protein metabolism indicators, nutritional status assessment, exercise and activity assessment, etc. There are many indicators that need to be tested and the testing methods are complicated. Summary of the invention

[0004] The present invention discovers a marker for skeletal muscle atrophy in pigs infected with Haemophilus parasuis, and provides a method and device for determining the meat production traits of pigs infected with Haemophilus parasuis by using the relative expression level of the marker, as well as a PCR detection primer set and a PCR detection kit used in the method for detecting the skeletal muscle atrophy of pigs infected with Haemophilus parasuis. The markers can be used to accurately evaluate the skeletal muscle atrophy of pigs infected with Haemophilus parasuis, thereby further evaluating the meat production traits of pigs infected with Haemophilus parasuis.

[0005] The technical solution provided by the present invention is specifically as follows:

[0006] In a first aspect, the present invention provides a PCR detection primer set for detecting skeletal muscle atrophy in pigs infected with Haemophilus parasuis, comprising:

[0007] Upstream primer of FOXO1: 5'-ACTCATTTGCGCCACCAAAC-3' (SEQ ID NO: 1),

[0008] Downstream primer of FOXO1: 5′-GAGTCCCGCTGCACAGTTAT-3′ (SEQ ID NO: 2);

[0009] Upstream primer of FBXO32: 5'-CGGCTCCTGTGGAAGAAACT-3' (SEQ ID NO: 3),

[0010] Downstream primer of FBXO32: 5′-GAGGGTAGCATCGCACAAGT-3′ (SEQ ID NO: 4);

[0011] TRIM63 upstream primer: 5'-AGGCTGCAAATCCCTACTGG-3' (SEQ ID NO: 5),

[0012] Downstream primer of TRIM63: 5′-TACACTCCGTGACGGTCCAT-3′ (SEQ ID NO: 6);

[0013] Upstream primer of CTSL: 5'-CTGGATCATGGCGTTCTGGT-3' (SEQ ID NO: 7),

[0014] Downstream primer of CTSL: 5′-GCCATCCATGCCCCAAGTAT-3′ (SEQ ID NO: 8);

[0015] Upstream primer of BNIP3: 5'-CTGGACATGACAGACGTCCC-3' (SEQ ID NO: 9),

[0016] Downstream primer of BNIP3: 5′-GCGTCCATGTGGGTAACAGA-3′ (SEQ ID NO: 10).

[0017] In a second aspect, the present invention provides a PCR detection kit for detecting skeletal muscle atrophy in pigs infected with Haemophilus parasuis, comprising the above primer set and taq enzyme.

[0018] In some embodiments provided by the present invention, the PCR detection kit for detecting skeletal muscle atrophy in pigs infected with Haemophilus parasuis provided by the present invention also includes an internal reference gene and primers thereof; the internal reference gene is GAPDH, and its upstream primer is: 5'-TGACATCAAGAAGGTGGTGAAG-3' (SEQ ID NO: 11), and the downstream primer is: 5'-TTGACGAAGTGGTCGTTGAG-3' (SEQ ID NO: 12).

[0019] In a third aspect, the present invention provides a method for determining the meat production traits of pigs infected with Haemophilus parasuis, comprising:

[0020] Obtaining the expression levels of markers of individuals infected with Haemophilus parasuis; the markers include at least FOXO1, FBXO32, TRIM63, CTSL and BNIP3;

[0021] The expression levels of markers in individuals infected with Haemophilus parasuis were compared with the average expression levels in healthy individuals;

[0022] Based on the comparison results, the skeletal muscle atrophy status of individuals infected with Haemophilus parasuis is determined, and the meat production traits are determined based on the skeletal muscle atrophy status.

[0023] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, the determining the skeletal muscle atrophy status of the Haemophilus parasuis infected individual according to the comparison result comprises: determining that the Haemophilus parasuis infected individual with skeletal muscle atrophy whose expression level of markers is higher than the average expression level of healthy individuals. The Haemophilus parasuis infected individual with skeletal muscle atrophy has poor meat production traits.

[0024] In some embodiments provided by the present invention, in the method for determining meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, when FOXO1 is 18.27 times higher than the average expression level of healthy individuals, FBXO32 is 6.24 times higher than the average expression level of healthy individuals, TRIM63 is 111.18 times higher than the average expression level of healthy individuals, CTSL is 3.08 times higher than the average expression level of healthy individuals, and BNIP3 is 3.55 times higher than the average expression level of healthy individuals, it is determined that Haemophilus parasuis infected individuals have skeletal muscle atrophy.

[0025] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, obtaining the expression level of the marker of the individual infected with Haemophilus parasuis comprises:

[0026] The cDNA and gene sequences of tissues from individuals infected with Haemophilus parasuis were obtained for Q-PCR amplification and 2 -ΔΔCTAnalyze the test results.

[0027] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, determining the skeletal muscle atrophy status of the individual infected with Haemophilus parasuis according to the comparison result comprises:

[0028] Skeletal muscle of individuals infected with Haemophilus parasuis whose expression levels of the markers were comparable to the average expression levels of healthy individuals did not atrophy.

[0029] In some embodiments provided by the present invention, the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention includes: selecting individuals with non-atrophic skeletal muscles for breeding. Individuals with non-atrophic skeletal muscles are less affected by Haemophilus parasuis infection, indicating that the individual may be resistant to Haemophilus parasuis, and the individual can be used to breed more pigs whose skeletal muscles do not atrophy after Haemophilus parasuis infection.

[0030] In a fourth aspect, the present invention provides a device for determining the meat production characteristics of pigs infected with Haemophilus parasuis, comprising:

[0031] An acquisition module, used to acquire the expression level of markers of individuals infected with Haemophilus parasuis; the markers at least include FOXO1, FBXO32, TRIM63, CTSL and BNIP3;

[0032] a comparison module for comparing the expression level of the marker in the individual infected with Haemophilus parasuis with the average expression level in the healthy individual;

[0033] A judgment module determines the meat production characteristics of the individual infected with Haemophilus parasuis based on the comparison result.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The present invention discovers for the first time that the expression of FOXO1, FBXO32, TRIM63, CTSL and BNIP3 is related to skeletal muscle atrophy in pigs infected with Haemophilus parasuis. After pigs are infected with Haemophilus parasuis, the expression levels of FOXO1, FBXO32, TRIM63, CTSL and BNIP3 are significantly increased compared with the average expression levels of healthy individuals, and skeletal muscle development is abnormal. That is, by detecting one or more of FOXO1, FBXO32, TRIM63, CTSL and BNIP3, the skeletal muscle atrophy of pigs infected with Haemophilus parasuis can be monitored and their meat production traits can be predicted, providing a new idea for dynamic guidance of pig production. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0037] Figure 1 The PCR amplification results of FOXO1, FBXO32, TRIM63, CTSL and BNIP3, the amplified fragments are 170 bp, 130 bp, 112 bp, 108 bp, 144 bp respectively;

[0038] Figure 2 A flow chart showing the construction of an in vivo model of Haemophilus parasuis infection in colostrum-fasted piglets is presented;

[0039] Figure 3 The Q-PCR results of FOXO1, FBXO32, TRIM63, CTSL and BNIP3 in the longissimus dorsi muscle of the normal group (Control) and the infected group (Treated) of piglets, * indicates P < 0.05, the difference is significant, ** indicates P < 0.01, the difference is extremely significant;

[0040] Figure 4 HE section results of the longest back measurement of piglets in the normal group and the Haemophilus parasuis infection group, * indicates P < 0.05, the difference is significant, ** indicates P < 0.01, the difference is extremely significant; A is the HE section image, and B is the statistical result. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] The PCR detection primer set provided by the present invention for detecting skeletal muscle atrophy in pigs infected with Haemophilus parasuis comprises:

[0043] Upstream primer of FOXO1: 5'-ACTCATTTGCGCCACCAAAC-3' (SEQ ID NO: 1),

[0044] Downstream primer of FOXO1: 5′-GAGTCCCGCTGCACAGTTAT-3′ (SEQ ID NO: 2);

[0045] Upstream primer of FBXO32: 5'-CGGCTCCTGTGGAAGAAACT-3' (SEQ ID NO: 3),

[0046] Downstream primer of FBXO32: 5′-GAGGGTAGCATCGCACAAGT-3′ (SEQ ID NO: 4);

[0047] TRIM63 upstream primer: 5'-AGGCTGCAAATCCCTACTGG-3 (SEQ ID NO: 5)',

[0048] Downstream primer of TRIM63: 5′-TACACTCCGTGACGGTCCAT-3′ (SEQ ID NO: 6);

[0049] Upstream primer of CTSL: 5'-CTGGATCATGGCGTTCTGGT-3' (SEQ ID NO: 7),

[0050] Downstream primer of CTSL: 5′-GCCATCCATGCCCCAAGTAT-3′ (SEQ ID NO: 8);

[0051] Upstream primer of BNIP3: 5'-CTGGACATGACAGACGTCCC-3' (SEQ ID NO: 9),

[0052] Downstream primer of BNIP3: 5′-GCGTCCATGTGGGTAACAGA-3′ (SEQ ID NO: 10).

[0053] The invention provides a PCR detection kit for detecting the skeletal muscle atrophy of pigs infected with Haemophilus parasuis, comprising the above primer set and taq enzyme.

[0054] In the present application, the taq enzyme is derived from SYBR Green Premix Ex Taq (RR420A, TaKaRa), and the amplification system contains: 5 μL SYBR Green Premix Ex Taq (RR420A, TaKaRa), 0.2 μL upstream primer, 0.2 μL downstream primer, 1 μL cDNA, and 3.6 μL pure water.

[0055] The reaction procedure of the PCR detection kit refers to the SYBR Green Premix Ex Taq instruction manual.

[0056] In some embodiments provided by the present invention, the PCR detection kit for detecting skeletal muscle atrophy in pigs infected with Haemophilus parasuis provided by the present invention also includes an internal reference gene and primers thereof; the internal reference gene is GAPDH, and its upstream primer is: 5'-TGACATCAAGAAGGTGGTGAAG-3' (SEQ ID NO: 11), and the downstream primer is: 5'-TTGACGAAGTGGTCGTTGAG-3' (SEQ ID NO: 12).

[0057] The method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention comprises:

[0058] Obtaining the expression levels of markers of individuals infected with Haemophilus parasuis; the markers include at least FOXO1, FBXO32, TRIM63, CTSL and BNIP3;

[0059] The expression levels of markers in individuals infected with Haemophilus parasuis were compared with the average expression levels in healthy individuals;

[0060] Based on the comparison results, the skeletal muscle atrophy status of individuals infected with Haemophilus parasuis is determined, and the meat production traits are determined based on the skeletal muscle atrophy status.

[0061] The term "at least includes" means that in addition to "FOXO1, FBXO32, TRIM63, CTSL and BNIP3", other possible markers are also included.

[0062] The term "healthy individual" refers to an individual who is not infected with any disease and does not suffer from skeletal muscle atrophy.

[0063] The individuals infected with Haemophilus parasuis include pigs that are currently infected with Haemophilus parasuis and / or pigs that have been infected with Haemophilus parasuis and cured.

[0064] The expression level includes mRNA content and / or protein expression.

[0065] The skeletal muscle atrophy condition includes whether skeletal muscle atrophy occurs and / or the degree of skeletal muscle atrophy.

[0066] The comparison results include quantitative comparison results or non-quantitative comparison results. Quantitative comparison results refer to the results after calculating the relative quantity, such as "FOXO1 is 18.27 times higher than the average expression level of healthy individuals"; non-quantitative comparison results refer to the results without calculating the specific amount of increase, such as "the expression level of the marker is higher than the average expression level of healthy individuals".

[0067] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, the determining the skeletal muscle atrophy status of the Haemophilus parasuis infected individual according to the comparison result comprises: determining that the Haemophilus parasuis infected individual with skeletal muscle atrophy whose expression level of markers is higher than the average expression level of healthy individuals. The Haemophilus parasuis infected individual with skeletal muscle atrophy has poor meat production traits.

[0068] In some embodiments provided by the present invention, in the method for determining meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, when FOXO1 is 18.27 times higher than the average expression level of healthy individuals, FBXO32 is 6.24 times higher than the average expression level of healthy individuals, TRIM63 is 111.18 times higher than the average expression level of healthy individuals, CTSL is 3.08 times higher than the average expression level of healthy individuals, and BNIP3 is 3.55 times higher than the average expression level of healthy individuals, it is determined that Haemophilus parasuis infected individuals have skeletal muscle atrophy.

[0069] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, the expression level of the marker of the individual infected with Haemophilus parasuis comprises: obtaining the tissue cDNA and gene sequence of the individual infected with Haemophilus parasuis for Q-PCR amplification, using 2 -ΔΔCT Analyze the test results.

[0070] In some embodiments provided by the present invention, in the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention, determining the skeletal muscle atrophy status of individuals infected with Haemophilus parasuis based on the comparison results includes: determining that the skeletal muscle of individuals infected with Haemophilus parasuis whose expression level of markers is equal to the average expression level of healthy individuals has not atrophied.

[0071] In some embodiments provided by the present invention, the method for determining the meat production traits of pigs infected with Haemophilus parasuis provided by the present invention includes: selecting individuals with non-atrophic skeletal muscles for breeding. Individuals with non-atrophic skeletal muscles are less affected by Haemophilus parasuis infection, indicating that the individual may be resistant to Haemophilus parasuis, and the individual can be used to breed more pigs whose skeletal muscles do not atrophy after Haemophilus parasuis infection.

[0072] The device provided by the present invention for determining the meat production characteristics of pigs infected with Haemophilus parasuis comprises:

[0073] An acquisition module, used to acquire the expression level of markers of individuals infected with Haemophilus parasuis; the markers at least include FOXO1, FBXO32, TRIM63, CTSL and BNIP3;

[0074] a comparison module for comparing the expression level of the marker in the individual infected with Haemophilus parasuis with the average expression level in the healthy individual;

[0075] A judgment module determines the meat production characteristics of the individual infected with Haemophilus parasuis based on the comparison result.

[0076] Through the description of the above implementation methods, those skilled in the art can clearly understand that the above method for determining the meat production characteristics of pigs infected with Haemophilus parasuis can be implemented by means of software plus a necessary general hardware platform, and of course, it can also be implemented by hardware, but in many cases the former is a better implementation method. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, can be embodied in the form of a software product, which is stored in a storage medium (such as ROM / RAM, magnetic disk, optical disk) as described above, and includes a number of instructions for enabling a terminal device to execute the methods described in each embodiment of the present application.

[0077] The technical solution of the present invention is described below through specific embodiments:

[0078] Example 1: Acquisition of FOXO1, FBXO32, TRIM63, CTSL and BNIP3.

[0079] The longissimus dorsi muscle tissue of 30-day-old Durbin×Chang×Dai piglets was collected, frozen in liquid nitrogen, and stored in a -80°C refrigerator. Tissue RNA was extracted using the TRIzol (DP424, TIANGEN) lysis method, and RNA was reverse transcribed using the PrimeScript RT reagent Kitwith gDNA Eraser (RR047A, TaKaRa) kit to obtain cDNA. The above operations were performed according to the reagent instructions. The cDNA obtained by reverse transcription was diluted with 60 μL of pure water (RR047A-6, TaKaRa) and stored in a -20°C refrigerator.

[0080] Based on the gene sequence of the pig genome (Sscrofa11.1), NCBI online software Primer-BLAST (https: / / www.ncbi.nlm.nih.gov / tools / primer-blast) was used to design PCR amplification primers for FOXO1, FBXO32, TRIM63, CTSL and BNIP3, respectively, as follows:

[0081] Table 1. Primer information

[0082]

[0083]

[0084] The above primer information was sent to a biological company to synthesize the corresponding sequence, and PCR amplification was performed using Premix Taq (RR901, TaKaRa) according to the reagent instructions. After PCR amplification, agarose gel electrophoresis was performed to analyze the size of the obtained fragment. The obtained fragment was consistent with the expected fragment size ( Figure 1 ). Further Sanger sequencing confirmed that the amplified fragments were partial sequences of porcine FOXO1, FBXO32, TRIM63, CTSL and BNIP3, confirming that these genes were truly expressed in pigs.

[0085] Example 2: The role of FOXO1, FBXO32, TRIM63, CTSL and BNIP3 as markers in skeletal muscle growth and development in fasted colostrum piglets infected with Haemophilus parasuis

[0086] like Figure 2 As shown in the figure, an in vivo model of Haemophilus parasuis infection in colostrum-fasting piglets was constructed. Briefly, newborn pigs without colostrum exposure and any immunization were sent to the animal research center for artificial feeding. After the common pathogen test was negative, 2×10 Haemophilus parasuis (SH0165 strain, serotype 5) were injected intratracheally into 30-day-old piglets. 8 CFU, 3 piglets were slaughtered 48 hours after infection and 3 piglets were not infected, and swabs of lung, peritoneum, pericardium, joint fluid and meningeal swabs were collected to detect bacterial invasion. The longissimus dorsi muscle tissue of these 6 piglets was quickly collected, one part was cut into slices, quickly frozen in liquid nitrogen and stored at low temperature, and the other part was cut into small pieces and fixed in 4% paraformaldehyde for histological analysis. The experimental animal operations were carried out in accordance with the "Hubei Province Laboratory Animal Management Regulations" (10 / 1 / 2005).

[0087] According to Example 1, tissue cDNA and gene sequences were obtained for Q-PCR amplification. The amplification system was as follows: 5 μL SYBR Green Premix Ex Taq (RR420A, TaKaRa), 0.2 μL upstream primer, 0.2 μL downstream primer, 1 μL cDNA, 3.6 μL pure water. The reaction procedure was referred to the SYBR Green Premix Ex Taq instruction manual. The internal reference gene was GAPDH, upstream primer: 5'-TGACATCAAGAAGGTGGTGAAG-3' (SEQ ID NO: 11), downstream primer: 5'-TTGACGAAGTGGTCGTTGAG-3' (SEQ ID NO: 12). Each sample was repeated 3 times, and 2 -ΔΔCT The test results were analyzed by the method, and the data were expressed as mean ± standard deviation.

[0088] The results are as follows Figure 3As shown in the figure, FOXO1, FBXO32, TRIM63, CTSL and BNIP3 were significantly highly expressed in the longissimus dorsi muscle of the infected pigs, suggesting that they are closely related to the growth and development of pig muscles. HE staining was performed on the longissimus dorsi muscles of the above two groups to analyze the muscle fiber diameter and area, and the data were expressed as mean ± standard deviation. Figure 4 As shown, the muscle fiber diameter and area of ​​the longissimus dorsi muscle of the infected piglets were significantly smaller than those of the normal group, that is, skeletal muscle atrophy occurred in the infected piglets. It can be predicted that the muscle weight of the infected group will decrease, which will reduce pork production.

[0089] Therefore, after being infected with highly toxic Haemophilus parasuis, piglets' skeletal muscle developed abnormally, and the five genes FOXO1, FBXO32, TRIM63, CTSL and BNIP3 were significantly overexpressed in the atrophied pig skeletal muscle. By detecting the expression level of one or several of these markers in pig skeletal muscle, the growth and development of pig skeletal muscle, especially whether muscle atrophy occurs, can be accurately judged, thus providing new ideas for dynamic guidance of pig production.

[0090] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A method for determining the meat production characteristics of pigs infected with Haemophilus parasuis, characterized in that: include: Obtaining the expression levels of markers of individuals infected with Haemophilus parasuis; the markers include at least FOXO1, FBXO32, TRIM63, CTSL and BNIP3; The expression levels of markers in individuals infected with Haemophilus parasuis were compared with the average expression levels in healthy individuals; According to the comparison results, the skeletal muscle atrophy status of the individuals infected with Haemophilus parasuis is determined, and the meat production trait is determined according to the skeletal muscle atrophy status; The method of determining the skeletal muscle atrophy of individuals infected with Haemophilus parasuis according to the comparison results includes: determining that the expression levels of the markers are all higher than the average expression levels of healthy individuals, indicating skeletal muscle atrophy in individuals infected with Haemophilus parasuis; when FOXO1 is 18.27 times higher than the average expression level of healthy individuals, FBXO32 is 6.24 times higher than the average expression level of healthy individuals, TRIM63 is 111.18 times higher than the average expression level of healthy individuals, CTSL is 3.08 times higher than the average expression level of healthy individuals, and BNIP3 is 3.55 times higher than the average expression level of healthy individuals, it is determined that the individuals infected with Haemophilus parasuis have skeletal muscle atrophy; The PCR primer set used to obtain the expression level of markers in individuals infected with Haemophilus parasuis includes: Upstream primer of FOXO1: 5'-ACTCATTTGCGCCACCAAAC-3', Downstream primer for FOXO1: 5′-GAGTCCCGCTGCACAGTTAT-3′; Upstream primer of FBXO32: 5'-CGGCTCCTGTGGAAGAAACT-3', Downstream primer of FBXO32: 5′- GAGGGTAGCATCGCACAAGT-3′; TRIM63 upstream primer: 5'-AGGCTGCAAATCCCTACTGG-3', Downstream primer of TRIM63: 5′-TACACTCCGTGACGGTCCAT-3′; Upstream primer of CTSL: 5'-CTGGATCATGGCGTTCTGGT-3', Downstream primer of CTSL: 5′- GCCATCCATGCCCCAAGTAT-3′; Upstream primer of BNIP3: 5'-CTGGACATGACAGACGTCCC-3', Downstream primer of BNIP3: 5′- GCGTCCATGTGGGTAACAGA-3′.

2. The method for determining the meat production characteristics of pigs infected with Haemophilus parasuis according to claim 1, characterized in that: The step of obtaining the expression level of the marker of an individual infected with Haemophilus parasuis comprises: The cDNA and gene sequences of tissues from individuals infected with Haemophilus parasuis were obtained for Q-PCR amplification and 2 -ΔΔCT Analyze the test results.

3. The method for determining the meat production characteristics of pigs infected with Haemophilus parasuis according to claim 1, characterized in that: Determining the skeletal muscle atrophy status of the individual infected with Haemophilus parasuis based on the comparison results comprises: Skeletal muscle of individuals infected with Haemophilus parasuis whose expression levels of the markers were comparable to the average expression levels of healthy individuals did not atrophy.

4. The method for determining the meat production characteristics of pigs infected with Haemophilus parasuis according to claim 3, characterized in that: Also includes: Individuals with no skeletal muscle atrophy are selected for breeding.

5. A device for determining the meat production characteristics of pigs infected with Haemophilus parasuis, characterized in that include: An acquisition module, used to acquire the expression level of markers of individuals infected with Haemophilus parasuis; the markers at least include FOXO1, FBXO32, TRIM63, CTSL and BNIP3; a comparison module for comparing the expression level of the marker in the individual infected with Haemophilus parasuis with the average expression level in the healthy individual; a judgment module, which determines the meat production characteristics of the individual infected with Haemophilus parasuis according to the comparison result; The method of determining the skeletal muscle atrophy of individuals infected with Haemophilus parasuis according to the comparison results includes: determining that the expression levels of the markers are all higher than the average expression levels of healthy individuals, indicating skeletal muscle atrophy in individuals infected with Haemophilus parasuis; when FOXO1 is 18.27 times higher than the average expression level of healthy individuals, FBXO32 is 6.24 times higher than the average expression level of healthy individuals, TRIM63 is 111.18 times higher than the average expression level of healthy individuals, CTSL is 3.08 times higher than the average expression level of healthy individuals, and BNIP3 is 3.55 times higher than the average expression level of healthy individuals, it is determined that the individuals infected with Haemophilus parasuis have skeletal muscle atrophy; The PCR primer set used to obtain the expression level of markers in individuals infected with Haemophilus parasuis includes: Upstream primer of FOXO1: 5'-ACTCATTTGCGCCACCAAAC-3', Downstream primer for FOXO1: 5′-GAGTCCCGCTGCACAGTTAT-3′; Upstream primer of FBXO32: 5'-CGGCTCCTGTGGAAGAAACT-3', Downstream primer of FBXO32: 5′- GAGGGTAGCATCGCACAAGT-3′; TRIM63 upstream primer: 5'-AGGCTGCAAATCCCTACTGG-3', Downstream primer of TRIM63: 5′-TACACTCCGTGACGGTCCAT-3′; Upstream primer of CTSL: 5'-CTGGATCATGGCGTTCTGGT-3', Downstream primer of CTSL: 5′- GCCATCCATGCCCCAAGTAT-3′; Upstream primer of BNIP3: 5'-CTGGACATGACAGACGTCCC-3', Downstream primer of BNIP3: 5′- GCGTCCATGTGGGTAACAGA-3′.

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