Method for detecting hydroxyl polycyclic aromatic hydrocarbons in urine and application thereof

By employing three-phase electroporation extraction technology and high-performance liquid chromatography, the complexity and inefficiency of detecting hydroxyl polycyclic aromatic hydrocarbons (HPAHs) in urine have been solved, resulting in a rapid, simple, and accurate detection method suitable for the efficient extraction and detection of HPAHs in human urine.

CN117929578BActive Publication Date: 2026-06-19HENAN BUSINESS SCI RES INST +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN BUSINESS SCI RES INST
Filing Date
2024-01-25
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing methods for detecting hydroxyl polycyclic aromatic hydrocarbons (PAHs) in urine are complex to operate, time-consuming, have insufficient purification capacity, consume large amounts of organic solvents, and have low sensitivity, making it difficult to quickly and accurately assess the association between PAH exposure and human health risks.

Method used

A three-phase electroporation extraction technique was employed, in which an organic extraction solvent was loaded onto a porous polymer membrane. An electric field was applied between the donor and receiver phases to achieve the active directional migration of hydroxyl polycyclic aromatic hydrocarbons, which were then detected by high-performance liquid chromatography.

Benefits of technology

It improves the mass transfer rate and extraction efficiency of the extraction process, shortens the time, simplifies the sample pretreatment process, and has a wide linear range, low detection limit and high spike recovery rate. It is suitable for accurate and highly sensitive detection of hydroxy polycyclic aromatic hydrocarbons in human urine.

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Abstract

This invention relates to the field of chemical analysis, specifically disclosing a method for detecting hydroxypolycyclic aromatic hydrocarbons (HPAs) in urine and its application. The method involves sampling and hydrolysis, followed by extraction of the crude extract using three-phase electrochemical membrane extraction (ECME). After extraction, high-performance liquid chromatography (HPLC) is used to detect the concentration of HPAs in the receiving phase. This invention simplifies the traditional pretreatment process for detecting HPAs in urine, simultaneously performing extraction separation, concentration enrichment, and purification using three-phase EME. The detection method provided by this invention features strong sample purification capabilities, fast analysis speed, high enrichment factor, high sensitivity, low detection limit, and environmental friendliness, making it more suitable for routine detection and analysis and showing broad application prospects in the detection field.
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