Compositions having dual soothing and scar repair efficacy, methods of making and use

Abstract

The application discloses a composition with double effects of soothing skin and repairing scars, a preparation method and application, and comprises acetylated chitosanamide, fibronectin and bird's nest acid with a mass ratio of (0.1-8):(0.01-5):(0.1-3.5). The composition with double effects of soothing skin and repairing scars is applied to skin care products. Compared with the prior art, the components of the application are matched and synergistically act, and can deeply achieve the effects of soothing skin and repairing scars.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a composition with the dual effects of soothing skin and repairing scars, and a preparation method and application thereof. BACKGROUND

[0002] The irradiation of ultraviolet rays and bad eating habits can cause damage to the skin barrier and the stratum corneum. After the stratum corneum is damaged, the skin's resistance to the outside world is weakened, and conditions such as redness, itching, and stinging are prone to occur. In order to alleviate this condition and improve the health status of the skin, it is necessary to develop a natural, mild, non-irritating, and skin-soothing skincare product as soon as possible. The definition of soothing is to improve the state of skin irritation through certain methods. The skin-soothing skincare product is not developed for sensitive skin, and non-sensitive skin can also use it, which has the remarkable feature of being mild and non-irritating.

[0003] At present, the skin-soothing skincare products circulating in the market are mainly some strain fermentation technology and fermentation products. However, there are still some deficiencies in the application of fermentation strains in cosmetics, such as the presence of many allergens due to the complex composition of fermentation products, so the sensitization rate is relatively high. For example, patent CN202211408726.X relates to a multiple strain fermentation filtrate and its preparation method and application, which discloses the effect of soothing skin, but the operation steps are complicated, the processing conditions are strict, the composition of the fermentation product is complex, and the product quality is difficult to control. Patent CN201410432411.8 relates to an anti-allergic soothing and repairing skin emulsion and its preparation method, which uses active substances in natural Chinese herbal medicines to achieve good calming and soothing skin effect, but the effect is single and does not have the effect of repairing scars. SUMMARY

[0004] The purpose of the present application is to overcome the defects of the prior art and provide a composition with the dual effects of soothing skin and repairing scars, and a preparation method and application thereof. The effective component composition is simple and the effect of each component is clear, and the production quality of the product is easy to control. The production process adopts microemulsion packaging technology, which promotes the transdermal performance of the effective components and can achieve the effects of soothing skin and repairing scars in a deep level.

[0005] The purpose of the present application can be achieved by the following technical solution: a composition with the dual effects of soothing skin and repairing scars, comprising acetylchitose, fibronectin, and bird's nest acid in a mass ratio of (0.1-8) :(0.01-5) :(0.1-3.5).

[0006] Further, the mass ratio of acetylchitose, fibronectin, and bird's nest acid is (0.1-8) :(0.05-5) :(2-3.5).

[0007] Further, the mass ratio of the chitosamine, fibronectin and cystosamine acid is (6-8):(0.05-3):(2-2.5).

[0008] Further, the mass ratio of the chitosamine, fibronectin and cystosamine acid is 6:(2.4-3):(2-2.5).

[0009] The application also provides a microemulsion containing the composition with the dual effects of soothing skin and repairing scars, which comprises the following components in parts by weight:

[0010] Glycerin 3-5;

[0011] Hydrogenated castor oil 1-3;

[0012] Propylene glycol 0.5-0.8;

[0013] Hexanediol 0.1-0.5;

[0014] Lecithin 2-8;

[0015] Cholesterol 0.5-1.5;

[0016] Caprylic / capric triglyceride 0.2-0.6;

[0017] Chitosamine 0.1-8;

[0018] Fibronectin 0.01-5;

[0019] Cystosamine acid 0.1-2.5;

[0020] Water 15-25.

[0021] The application also provides a preparation method of the microemulsion, which comprises the following steps:

[0022] S01, chitosamine, fibronectin and cystosamine acid are added into water and heated to 50-80 DEG C to obtain E liquid;

[0023] S02, glycerin, hydrogenated castor oil, propylene glycol, hexanediol, lecithin, cholesterol and caprylic / capric triglyceride are mixed and dissolved to prepare a solution, and the solution is heated to 70-90 DEG C to obtain F liquid;

[0024] S03, E liquid and F liquid are mixed and high-pressure homogenized at high speed, and cooled to 30-50 DEG C to obtain the microemulsion.

[0025] The application also provides a skin care product containing the microemulsion, and the microemulsion accounts for 30-40% of the total mass of the skin care product.

[0026] Further, the microemulsion comprises 0.1-8% acetylated chitosamine, 0.001%-5% fibronectin and 0.1-3.5% bird's nest acid in total mass of the skin care product.

[0027] Further, the skin care product comprises emulsion, cream, freeze-dried powder or skin care water.

[0028] Further, the skin care product is cream, which comprises 30-40% microemulsion and auxiliary materials in total mass of the skin care product.

[0029] Further, the auxiliary materials comprise phase A, phase B, phase C, phase D,

[0030] Phase A comprises:

[0031] Sodium acrylate / sodium acryloyldimethyltaurate copolymer 3-4%;

[0032] Xanthan gum 4-5%;

[0033] Hydroxymethylcellulose 0.2-0.6%;

[0034] Phenoxyethanol 0.2-0.6%;

[0035] Pentylene glycol 0.2-0.6%;

[0036] EDTA-2NA 0.02-0.06%;

[0037] Solvent balance;

[0038] Phase B comprises:

[0039] Kaolin 2-3%;

[0040] Titanium dioxide 2-3%;

[0041] Phase C comprises:

[0042] Phytosteryl 3-5%;

[0043] Squalane 1-2%;

[0044] Tocopheryl acetate 4-5%;

[0045] Behenyl alcohol 1-2%;

[0046] Isopropyl isononanoate 5-6%;

[0047] Phase D comprises:

[0048] PEG-100 stearate 1-3%

[0049] Daily fragrance 0.02-0.06%.

[0050] Further, the solvent is water.

[0051] The application further provides a preparation method of the skin care product, comprising the following steps:

[0052] S11, adding the remaining components of phase A into water, wetting and dispersing completely to obtain a mixture;

[0053] S12, adding the components of phase B and phase C into the mixture obtained in step S11) and corresponding prepared microemulsion, heating to 50-70 DEG C, homogenizing for 3-5 minutes to make it disperse completely;

[0054] S13, adding the components of phase D into the product obtained in step S12), homogenizing for 1-5 minutes to make it emulsify completely, heating to 50-70 DEG C, to obtain a cream product with the dual effects of soothing skin and repairing scars.

[0055] The homogenizing conditions in steps S12 and S13 are both 2000-5000 r / min.

[0056] Compared with the prior art, the application has the following beneficial effects:

[0057] 1, the application combines acetylated chitosamine, fibronectin and bird's nest acid in a specific weight ratio to form a composition, and the three functional substances in the composition have the synergistic effect of soothing skin and repairing scars, which can play a significant effect of soothing and repairing, compared with the weak effect of soothing and repairing when using any one or two of the three functional substances.

[0058] 2, the composite composition selected by the application has the dual effects of soothing skin and repairing scars. The composition of the composite composition is simple in component type and clear in effect, has good soothing effect on skin and good repairing effect on scars, can achieve multiple skin care and beautifying effects including enhancing skin elasticity and moisturizing and soothing skin, and has great market competitiveness.

[0059] 3, the components for composing the composite composition of the application are mild and non-irritating. The production process is clear and simple to operate, which is convenient for controlling the quality of the product and the scale of production. The application of microemulsion encapsulation technology can ensure the effects of soothing skin and repairing scars.

[0060] 4、The present application in order to better enhance the transdermal performance of the active ingredient, to achieve good skin soothing and repair scar skin care effect, the preparation method of the microemulsion mentioned in the present application adopts the wrapping technology of microemulsion, the microemulsion as a carrier has excellent characteristics such as histocompatibility, cell affinity, targeting, sustained release and controlled release, and the effective components are loaded in the microemulsion, which can effectively and fully utilize the related effective components, and the human body efficacy test results show that the microemulsion raw material has a slight skin soothing effect. BRIEF DESCRIPTION OF DRAWINGS

[0061] Figure 1 The scar area reduction rate of rabbits using application examples 1-5 and comparative application examples 1-7 is compared. DETAILED DESCRIPTION

[0062] In order to facilitate the understanding of the present application, the present application will be described in more detail below with reference to relevant examples. The following gives the preferred embodiments of the present application. However, the present application can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0063] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the description of the present application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application.

[0064] In the present application, the technical features described in an open manner include both the closed technical solution consisting of the listed features and the open technical solution containing the listed features.

[0065] In the present application, when a numerical interval is involved, the above numerical interval is considered to be continuous and includes the minimum value and the maximum value of the range, as well as every value between the minimum value and the maximum value, unless otherwise specified. Further, when the range is an integer, every integer between the minimum value and the maximum value of the range is included. In addition, when multiple ranges are provided to describe a feature or a characteristic, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges falling within the range.

[0066] Only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form a range not explicitly recited; and any lower limit can be combined with any other lower limit to form a range not explicitly recited, as can any upper limit with any other upper limit to form a range not explicitly recited. Further, each individual disclosed point or single numerical value can itself serve as a lower limit or upper limit to combine with any other point or single numerical value or with other lower limits or upper limits to form a range not explicitly recited.

[0067] The temperature parameter in the present application, if not particularly limited, allows for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows for fluctuations within the accuracy of the instrument control.

[0068] In the description of the application, the meaning of "plurality" is at least two, for example, two, three, etc., unless specifically limited.

[0069] If not specifically stated, all embodiments and optional embodiments of the present application can be combined to form new technical solutions. If not specifically stated, all technical features and optional technical features of the present application can be combined to form new technical solutions.

[0070] If not specifically stated, all steps of the present application can be performed in sequence or randomly, preferably in sequence.

[0071] The present application provides a skin care product with the dual functions of soothing the skin and repairing scars. The components in the skin care product have clear functions and coordinate with each other, specifically including:

[0072] The content of acetylated chitosamine is 0.1%-8% based on the total mass of the product, which is extracted from shrimp shells. Since the raw materials are easy to obtain and the preparation scheme is clear, the production cost is low and the quality of acetylated chitosamine is easy to control.

[0073] The content of fibronectin is 0.001%-5% based on the total mass of the product, which is selected from external purchase. Since the production means of the factory on the market is clear and skilled, bulk purchase can reduce the cost and the quality of fibronectin is easy to control. Preferably, the content of fibronectin is 0.05-5%.

[0074] The content of bird's nest acid is 0.1%-3.5% based on the total mass of the product, which is selected from external purchase. Since the production means of the factory on the market is clear and skilled, bulk purchase can reduce the cost and the quality of bird's nest acid is easy to control. Preferably, the content of bird's nest acid is 2-3.5%.

[0075] The three functional components are combined in a specific weight ratio to form a composition that can exert significant soothing and repairing effects, wherein:

[0076] The chitosan can be obtained from shrimp shells. After removing the protein and minerals from the shrimp shells, the mixture containing chitosan is obtained by acid hydrolysis or enzymatic hydrolysis, and the high-purity chitosan is obtained by purifying the mixture.

[0077] (NAG is chitosan-structural formula attached)

[0078] Chitosan, also known as NAG, is a monosaccharide similar to glucose, which is widely present in animals, plants and human bodies in the form of chitin. NAG has high stability, high safety, high water solubility and high moisturizing property, can be produced on a large scale, and the production scale and quality are easy to control. NAG can quickly penetrate the stratum corneum and penetrate into the skin bottom layer, has an anti-inflammatory effect of reducing pain. NAG is a natural moisturizing agent, can gently promote keratin metabolism, enhance skin hydration, and make the skin more transparent. Long-term use of NAG can strengthen the skin barrier and enhance the skin's defense function against ultraviolet rays. NAG can also promote wound healing, reduce wound infection, promote the production of hyaluronic acid, and improve the appearance of the skin. It is one of the effective ingredients for soothing the skin and repairing scars.

[0079] Fibronectin, also known as fibronectin, adhesion protein and fibronectin, is a widely distributed multi-domain glycoprotein present in most extracellular matrices. Fibronectin can bind to cell surface receptors and collagen, proteoglycans and other adhesion molecules at the same time. This property enables it to adhere to cells and nutrients, transport nutrients required for cell metabolism, growth and proliferation (such as collagen, hyaluronic acid, small molecule peptides, glycoproteins, etc.), promote cell adhesion and proliferation, repair damaged cells, activate cells with insufficient activity, stimulate cells to secrete various functional proteins and promote cell proliferation. It has a wide application in regenerative medicine. Fibronectin has a connecting effect, can bind heparin, collagen, fibers, cells and 11 kinds of integrins, and plays an important role in wound repair, cancer diagnosis, cell culture and other aspects. Fibronectin also has moisturizing effect, which is beneficial to soothing and soothing the skin, and can achieve certain skin care and beautifying effect. In wound healing, fibronectin participates in blood coagulation, anti-inflammatory, granulation tissue production and tissue reconstruction, and plays an important role. At present, in the treatment of difficult-to-heal severe wounds, large-scale addition of exogenous fibronectin is usually used to help wound healing and improve skin appearance. During the growth of damaged tissues, fibronectin increases the expression of integrins, thereby accelerating the repair of tissues and scars. It is one of the effective ingredients for soothing the skin and repairing scars.

[0080] Cystosamine, fibronectin and edestin in the efficacy component of the application have synergistic effect, which can significantly exert soothing and repairing effect, and can achieve multiple skin care and beautifying effects including enhancing skin elasticity and moisturizing soothing skin care.

[0081] The synergistic effect of acetylchitose, fibronectin and edestin in the efficacy component of the application can significantly exert soothing and repairing effect, and can achieve multiple skin care and beautifying effects including enhancing skin elasticity and moisturizing soothing skin care.

[0082] Meanwhile, the microemulsion is used as a carrier, which has excellent properties such as tissue compatibility, cell affinity, targeting, sustained release and controlled release, and the effective components are encapsulated in the microemulsion, which can effectively and sufficiently utilize the related effective components and ensure the soothing and repairing effects of the skin and scars.

[0083] The following will be further described in detail through specific examples of the combination having soothing and repairing effects of the skin and scars, the preparation method and application. The following examples are more specific, and it can be understood that in other examples, it is not limited thereto. In the following specific examples, the instruments, reagents and materials involved are conventional instruments, reagents and materials in the prior art, which can be obtained through regular commercial channels, unless otherwise specified. The experimental methods and detection methods involved in the following examples are conventional experimental methods and detection methods in the prior art, unless otherwise specified.

[0084] Cystosamine is purchased from Xi'an Hulin Biological Technology Co., Ltd., model number 10036-64-3.

[0085] Fibronectin is purchased from Beijing Eppendorf Biological Technology Co., Ltd., model number FN.

[0086] Edestin is purchased from Snowe Biological Technology Co., Ltd.

[0087] Example 1

[0088] A composite composition having soothing and repairing effects of the skin and scars contains effective components having soothing and repairing effects of the skin and scars, and the content of acetylchitose is 4 parts, the content of fibronectin is 1.4 parts, and the content of edestin is 3.5 parts.

[0089] The above-mentioned compound composition with the dual functions of soothing skin and repairing scars is made into a microemulsion, and the specific preparation method is as follows:

[0090] (1) 4 parts of acetylated chitosan, 1.4 parts of fibronectin and 3.5 parts of bird's nest acid are added into 16.2 parts of water, and heated to 60°C to obtain E liquid;

[0091] (2) 4 parts of glycerol, 2 parts of hydrogenated castor oil, 0.6 parts of propylene glycol, 0.4 parts of hexylene glycol, 4 parts of lecithin, 1 part of cholesterol and 0.4 parts of caprylic / capric triglyceride are mixed and dissolved to prepare a solution, and heated to 80°C to obtain F liquid;

[0092] (3) The E liquid and the F liquid are mixed, and high-speed high-pressure homogenization is performed, and the temperature is reduced to 40°C to obtain a microemulsion.

[0093] The formulations of Examples 2-5 and Comparative Examples 1-7 are shown in Table 1 below, and the preparation method of Examples 2-5 is referred to Example 1.

[0094] The preparation method of Comparative Examples 1-7 is referred to Example 1, and if it does not involve a certain component (the content in Table 1 is 0), it means that the component is not added.

[0095] The product prepared in Comparative Example 7 does not form a microemulsion structure.

[0096] Table 1 Formulation table of Examples 1-5 and Comparative Examples 1-7 (the content of each component is "parts by weight")

[0097]

[0098]

[0099] The above-mentioned each example and comparative example is applied in skin care products, and the following takes the preparation of a cream as an example to be described in detail.

[0100] Application Example 1

[0101] The application example provides a cream with the functions of soothing skin and repairing scars, and the formulation is shown in Table 2 as follows:

[0102]

[0103]

[0104] The preparation method of the cream with the functions of soothing skin and repairing scars provided in the application example is as follows:

[0105] 1) The remaining components of the A phase are added into water to wet and disperse completely to obtain a mixed solution;

[0106] 2) To the mixture obtained in step 1), add each component of phase B, phase C and the functional ingredient (microemulsion of Example 1), heat to 60°C, and homogenize at 3000 r / min for 3-5 minutes to make it completely dispersed;

[0107] 3) To the product obtained in step 2), add each component of phase D, homogenize at 3000 r / min for 3 minutes to make it completely emulsified, heat to 60°C, to obtain a cream composition with the effect of improving skin gloss.

[0108] Application Example 2-5

[0109] The cream provided in Application Example 2-5 has the same formulation as that of Application Example 1, except that the microemulsion of Example 1 is replaced with an equal amount of the microemulsion of Example 2-5, and other components remain unchanged. The preparation method is referred to Application Example 1. (Application Examples 2-5 correspond to the addition of the microemulsion of Examples 2-5, respectively.)

[0110] Comparative Application Example 1-6

[0111] Seven creams are provided in Comparative Application Example 1-6, which have the same formulation as that of Application Example 1, except that the microemulsion of Example 1 is replaced with an equal amount of the microemulsion of Comparative Example 1-6, and other components remain unchanged. (Comparative Application Examples 1-6 correspond to the addition of the microemulsion of Comparative Examples 1-6, respectively.)

[0112] Comparative Application Example 7

[0113] Comparative Application Example 7 provides a cream, which has the same formulation as that of Application Example 1, except that the microemulsion of Example 1 is replaced with an equal amount of the product prepared according to Comparative Example 7 in Table 1.

[0114] Effect Verification Test Example

[0115] 1. Human efficacy test:

[0116] (1) Subjects: 120 healthy volunteers, all female, aged 20-55, divided into 12 groups, 10 people in each group.

[0117] The subjects included were healthy volunteers from Shanghai, who had no skin diseases or other major diseases in the past three months, and had not taken any antibiotics or hormone drugs. All subjects had provided written informed consent before the study, and after full communication, the subjects had signed and confirmed. The whole process of the study was strictly supervised and guided by the ethics committee, and the personal privacy information and data security of the subjects were strictly protected.

[0118] Environmental conditions: The experimental environmental conditions (temperature 20-22°C, humidity 40-60%) were set according to the experimental requirements, and the subjects were adapted to the test environment for more than 15-30 minutes.

[0119] (2) Test instrument: Mexameter (Courage & Khazaka GmbH, MX18, Cologne, Germany), test indicators are shown in Table 3 below:

[0120]

[0121] (3) Test method:

[0122] The instrument was used to test the volunteers. The hemoglobin content of the corresponding facial skin of each subject was tested before the trial (D0 in Table 3), 2 weeks (W2 in Table 3), 4 weeks (W3 in Table 3), and 8 weeks (W4 in Table 3) after the trial, respectively. After obtaining the corresponding data, the data were analyzed and arranged to obtain the corresponding change rate based on the pre-trial

[0123] (4) Data analysis:

[0124] Each group of subjects used the same cream (i.e., the test product prepared in the above application examples or comparative application examples). After calculating the average value of the test data of each subject in each group, the D0 average value data measured before using the test product of different application examples or comparative application examples were compared, and the average change rate of the related parameters was calculated. If the change rate remains a certain increase or decrease over a long period of time, it has a certain universality.

[0125] (5) Instrument data processing and analysis

[0126] Analysis of the change of the hemoglobin content of the skin of the subjects over time:

[0127] The instrument Mexameter can obtain the amount of emitted light and the amount of light absorbed by the skin of each subject between each application example and the comparative application example. After digital processing, the skin hemoglobin E value can be obtained. The average E value data of the skin of each of the 10 subjects in each group before and after using the test product is shown in Table 4:

[0128] Table 4 Average change of hemoglobin content of the skin

[0129]

[0130] From Table 4, it can be seen that after using the test products in application examples 1-5 and comparative application examples 1-7, the average hemoglobin content of the skin of the subjects has a general decrease compared to the other groups, and the hemoglobin content of the skin using the samples of application examples 1-3 has a higher decrease rate, that is, application examples 1-3 have a better effect on improving the hemoglobin content of the skin. The above results suggest that under the test conditions in this test, the test product of application example 3 can significantly reduce the hemoglobin content of the skin and has the effect of soothing the skin.

[0131] Note: According to the comparison of the data of each application example, application example 3 has the most significant effect of reducing the hemoglobin content of the skin, so each comparative application example is set based on application example 3. As can be seen from Table 4, the product used in application example 3 has the most significant effect of reducing the hemoglobin content of the skin, and has the effect of soothing the skin.

[0132] 2. Animal test of inhibiting scar proliferation (rabbit ear model)

[0133] The main purpose of this test is to study the effect of the test products prepared by each application example and comparative application example on the scar area of rabbits.

[0134] 60 healthy New Zealand rabbits (weight 2.0-3.0 kg, gender not limited) were used in the test, and were evenly divided into 10 groups: application example 1 (5 rabbits), application example 2 (5 rabbits), application example 3 (5 rabbits), application example 4 (5 rabbits), application example 5 (5 rabbits), comparative application example 1 (5 rabbits), comparative application example 2 (5 rabbits), comparative application example 3 (5 rabbits), comparative application example 4 (5 rabbits), comparative application example 5 (5 rabbits), comparative application example 6 (5 rabbits), and comparative application example 7 (5 rabbits). Square puncture wounds (side length = 1 cm) were made on the ventral side of both ears of each rabbit to create a scar model.

[0135] (Note: The unit of scar area is cm 2 )

[0136] Treatment steps:

[0137] Starting from the 14th day of the wound, the cream products prepared by application examples 1-5 and comparative application examples 1-7 (i.e. test products) were used to treat the wounds every day for 10 days. On the 30th day, the scar area of each rabbit was measured, and the average value was expressed. Based on the initial (without applying any test product) scar area, the average change rate of the scar area of each group of 5 rabbits using the cream prepared by application examples 1-5 and comparative application examples 1-7 was calculated, respectively, as shown in Table 5. Figure 1 .

[0138] From the results of this test, it can be seen that the average scar area of the rabbits treated with each test product is lower than that measured without applying the test product. Among them, the average reduction rate of the scar area using the test products of application examples 1-3 is higher, that is, application examples 1-3 have better effect on inhibiting scar proliferation, especially application example 3, which has a scar proliferation inhibition change rate of 36.78%.

[0139] From the processed experimental data, it can be seen that the scar area of all rabbits in the test has decreased, and Figure 1Among all the groups, the application example 3 is the most effective in reducing the scar area, and thus the test product applied in the application example 3 has the most repairing effect on the scar.

[0140] The above description of the embodiments is to assist the person of ordinary skill in the art to understand and use the application. Those skilled in the art can easily make various modifications to the embodiments and apply the general principles described herein to other embodiments without creative labor. Therefore, the application is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art based on the disclosure of the application without departing from the scope of the application should be within the protection scope of the application.

Claims

1. A microemulsion of a composition having dual effects of soothing the skin and repairing scars, characterized in that, Includes the following components in parts by weight: Glycerin 3~5; 1-3g of hydrogenated castor oil; Propylene glycol 0.5~0.8; Hexanediol 0.1~0.5; Lecithin 2-8; Cholesterol 0.5-1.5 Caprylic / capric triglycerides 0.2~0.6; Acetyl glucosamine 0.1~8; Fibronectin 0.05~5; Sialic acid 2-3.5%; Water 15~25.

2. A method for preparing a microemulsion as described in claim 1, characterized in that, Includes the following steps: S01. Add acetyl glucosamine, fibronectin and sialic acid to water and heat to 50~80℃ to obtain solution E; S02. Glycerin, hydrogenated castor oil, propylene glycol, hexanediol, lecithin, cholesterol, and caprylic / capric triglycerides are mixed and dissolved to prepare a solution, which is then heated to 70~90℃ to obtain solution F. S03. Mix liquid E and liquid F, homogenize under high speed and high pressure, and cool to 30~50℃ to prepare microemulsion.

3. A skincare product, characterized in that, The skin care product contains the microemulsion described in claim 1, wherein the microemulsion accounts for 30-40% of the total mass of the skin care product.

4. The skincare product according to claim 3, characterized in that, The skincare products include lotions, creams, freeze-dried powders, or skincare toners.

5. The skincare product according to claim 3, characterized in that, The skincare product mentioned is a face cream, which includes 30-40% microemulsion and excipients by weight.

6. The skincare product according to claim 5, characterized in that, Taking the total mass of the skincare product as 100, the excipients include phase A, phase B, phase C, and phase D. Phase A includes: 3-4% sodium acrylate / sodium acryloyl dimethyl taurate copolymer; Xanthan gum 4-5%; Hydroxymethyl cellulose 0.2~0.6%; Phenoxyethanol 0.2~0.6%; Pentylene glycol 0.2~0.6%; EDTA-2NA 0.02~0.06%; Water balance; Phase B includes: 2-3% kaolin; Titanium dioxide 2-3%; Phase C includes: Phytosterols 3-5%; Squalane 1~2%; Tocopheryl acetate 4-5%; Behenol 1-2%; Isonononyl isononanoate 5-6%; Phase D includes: PEG-100 stearate 1~3% Daily-use fragrance 0.02~0.06%.

7. A method for preparing a skincare product as described in claim 6, characterized in that, Includes the following steps: S11. Add the remaining components of phase A to the solvent, and wet and disperse them completely to obtain a mixture; S12. Add the components of phase B and phase C and the corresponding microemulsions to the mixture obtained in step S11), heat to 50~70℃, and homogenize for 3-5 minutes to ensure complete dispersion. S13. Add each component of phase D to the product obtained in step S12, homogenize for 1-5 minutes to ensure complete emulsification, and heat to 50-70℃ to obtain a face cream product with dual effects of soothing the skin and repairing scars.

8. The method for preparing the skincare product according to claim 7, characterized in that, The homogenization conditions in steps S12 and S13 are both 2000 ~ 5000 r / min.

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