A soil treatment composition

Abstract

The application discloses a soil treatment composition, a preparation method and an application scheme thereof, and discloses a composition comprising methyl isothiocyanate compounds and fluopyram, wherein the methyl isothiocyanate compounds are selected from dithiocarbamates or metam, the weight ratio of the two is 400:1-1400:1, and the weight percentage of active ingredients in the soil treatment composition is 5%-99%. The composition has high activity on a series of nematodes and soil-borne fungal diseases which invade crops, and has significant synergistic performance on Fusarium and Meloidogyne in particular, and meanwhile, the composition can meet the basic prevention and treatment requirements while reducing the amount of application.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of pesticides, and particularly relates to a soil treatment composition and preparation and application thereof. BACKGROUND

[0002] Soil health is the basis for sustainable agricultural development, but with the continuous cultivation of soil, a series of health problems are caused, in which the original microbial system of the soil is destroyed, and harmful nematodes, fungi and bacteria multiply in large numbers to become a prominent problem in agricultural development in recent years.

[0003] In the current agricultural production, the treatment of harmful microorganisms in the soil by using soil treatment agents is still the most common method, and with the gradual prohibition of high-toxicity products, dazomet and metam is becoming the mainstream product in soil treatment. However, dazomet and metam have problems such as large dosage, short prevention effect, and inconvenient operation, which limit the development of soil treatment technology.

[0004] The prior art CN104244714A discloses the use of succinate dehydrogenase inhibitors for controlling wood diseases in grapevines, and lists a large number of commercial fungicidal active substances including dazomet in the specification. The control target of the patent is fungal diseases (mainly air-borne diseases) on grape crops, and the use of dazomet to control air-borne fungal diseases needs to overcome certain technical difficulties, but the patent does not study the combination mode, mechanism of action, application method and other aspects of the two.

[0005] The prior art CN102696610A discloses a large number of compositions of fluopyram in combination with known insecticides, and mentions in the specification that it can be combined with further fungicidal active substances such as dazomet or metam. It is difficult for those skilled in the art to predict that the multi-component active ingredient combination has a synergistic effect on all diseases, and the patent only controls diseases and pests, and does not solve the problem of soil disease control.

[0006] The prior art CN103298341A discloses a method for controlling nematodes and / or increasing crop yield in nematode-resistant crops using fluopyram and its composition, and lists a large number of active substance compositions of fluopyram in combination with dazomet or metam in the specification. The control object of the patent examples is plant seeds, and the problem of soil disease control is not solved.

[0007] To solve the above problems, the application provides a high-efficiency soil treatment scheme, the combination of methyl isothiocyanate compounds and succinate dehydrogenase inhibitor fluopyram can produce a synergistic effect on the control of nematodes and soil-borne fungal diseases, can significantly reduce the use amount of dazomet and benefin, improve the distribution uniformity of the pesticide application, prolong the effective time after soil treatment, and effectively control the spread of nematodes and soil-borne fungal diseases in the soil, since dazomet and benefin also have a preventive effect on bacteria, and can simultaneously prevent bacterial diseases, and a good effect of soil treatment is achieved. SUMMARY

[0008] The application aims to provide an excellent soil treatment composition.

[0009] Another object of the application is to provide the application of the composition in soil treatment, which can be used for preventing and treating crop soil-borne fungal diseases and soil-borne nematode diseases.

[0010] The composition of the application has a synergistic effect, and the availability and persistence are better than those of a single agent, the use amount of the pesticide can be greatly reduced, the generation of target drug resistance is slowed down, the pesticide residue is reduced, and the comprehensive management of agricultural diseases is facilitated.

[0011] To achieve the above object, the application provides the following technical scheme.

[0012] A soil treatment composition comprises methyl isothiocyanate compounds and fluopyram, and the methyl isothiocyanate compounds are selected from dazomet or benefin.

[0013] The mass ratio of the methyl isothiocyanate compounds to fluopyram is 400:1-1400:1, and the sum of the weights accounts for 5%-99% of the total weight;

[0014] Further, the mass ratio of the methyl isothiocyanate compounds to fluopyram is 460:1-1210:1, and the sum of the weights accounts for 10%-98% of the total weight;

[0015] Further, the methyl isothiocyanate compounds are dazomet, the mass ratio of dazomet to fluopyram is 650:1-1050:1, and the sum of the weights accounts for 20%-95% of the total weight;

[0016] Further, the methyl isothiocyanate compounds are benefin, the mass ratio of benefin to fluopyram is 520:1-820:1, and the sum of the weights accounts for 20%-60% of the total weight;

[0017] The soil treatment composition provided by the present application can be made into any dosage form of agricultural use with appropriate adjuvants, and the preferred dosage form is water emulsion, suspension, dispersible oil suspension, suspension emulsion, water dispersible granule and granule, etc. according to various factors such as the crops to be controlled, environmental conditions, control methods, control costs, etc.

[0018] The soil treatment composition of the present application also contains common adjuvants required for formulating pesticide formulations, wherein the solid formulations include dispersants, wetting agents, antifoaming agents, complexing agents, pH adjusting agents and fillers, etc., and the liquid formulations include dispersants, emulsifiers, wetting agents, stabilizers, thickening agents, pH adjusting agents, antifoaming agents and antifreezing agents, etc.

[0019] The wetting agent is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethoxylate, tallow amine ethoxylate, alkyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether sulfate, acyl glutamate.

[0020] The dispersant is selected from one or more of condensed naphthalene sulfonate, sodium salt of phenol sulfonic acid condensate, sodium salt of methyl naphthalene sulfonate formaldehyde condensate, sodium lignosulfonate, sodium salt of methylene dinaphthalene sulfonate, sodium salt of acrylic acid homopolymer, sodium salt of dioctyl sulfosuccinate, sodium salt of maleic acid-acrylic acid copolymer.

[0021] The emulsifier is selected from one or more of alkyl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil ethylene oxide adduct and its derivatives, alkyl sulfonate, alkyl diphenyl ether sulfonate, naphthalene sulfonic acid formaldehyde condensate, alkyl phenol polyoxyethylene ether formaldehyde condensate, polyoxyethylene polyoxypropylene block copolymer, alkyl naphthalene sulfonic acid formaldehyde condensate, quaternary ammonium salt, tallow amine ethoxylate, amino acid, amine oxide, betaine and acyl glutamate.

[0022] The present application also provides the application of the soil treatment composition in the control of soil-borne fungal diseases and nematode diseases of crops.

[0023] The soil treatment composition provided by the present application can be used for the control of fungal diseases of crops, and the fungal diseases are soil-borne fungal diseases, and the pathogenic bacteria of the diseases are selected from Phytophthora, Pythium, Phoma, Rhizopus, Rhizoctonia, Verticillium and Sclerotinia.

[0024] The soil treatment composition provided by the present application can be used for the control of fungal diseases of crops, and the fungal diseases are soil-borne fungal diseases, and the pathogenic bacteria of the diseases are selected from Phytophthora, Pythium, Phoma, Rhizopus, Rhizoctonia, Verticillium and Sclerotinia.

[0025] Phytophthora infection causes soil-borne fungal crop diseases selected from the group consisting of tomato root rot, pepper bacterial wilt, pepper gray bacterial wilt, pumpkin bacterial wilt, bean bacterial wilt, onion white bacterial wilt, garlic bacterial wilt, and the like.

[0026] Pythium infection causes soil-borne fungal crop diseases selected from the group consisting of tomato damping-off, tomato damping-off, eggplant damping-off, cucumber damping-off, melon damping-off, loofah damping-off, zucchini damping-off, green vegetable damping-off, green vegetable damping-off, green vegetable black leg, cauliflower damping-off, cabbage damping-off, rape damping-off, Chinese cabbage damping-off, spinach damping-off, sugar beet damping-off, and the like; soybean damping-off, and the like.

[0027] Phoma infection causes soil-borne fungal crop diseases selected from the group consisting of Chinese cabbage black leg, green vegetable black leg, cabbage black leg, rape black leg, cauliflower black leg, tobacco black leg, celery black leg, and the like; potato black leg, and the like.

[0028] Rhizopus infection causes soil-borne fungal crop diseases selected from the group consisting of balsam pear root rot, pumpkin root rot, pea black root rot, and the like.

[0029] Rhizoctonia infection causes soil-borne fungal crop diseases selected from the group consisting of tomato damping-off, tomato stem base rot, pepper damping-off, bean stem base rot, cauliflower black root rot, cabbage black root rot, green vegetable damping-off, and the like; soybean damping-off, and the like.

[0030] Verticillium infection causes soil-borne fungal crop diseases selected from the group consisting of tomato yellow wilt, eggplant yellow wilt, pepper yellow wilt, and the like.

[0031] Sclerotinia infection causes soil-borne fungal crop diseases selected from the group consisting of tomato sclerotinia, pepper sclerotinia, cucumber sclerotinia, balsam pear sclerotinia, bean sclerotinia, cowpea sclerotinia, green vegetable sclerotinia, cauliflower sclerotinia, purple cabbage sclerotinia, celery sclerotinia, lettuce sclerotinia, onion small sclerotinia, and the like; soybean sclerotinia, and the like.

[0032] Soil-borne fungal diseases of crops caused by *Fusarium* species include: root rot of oats, wheat, corn, barley, potato dry rot, potato root rot, potato wilt, sweet potato root rot, and sweet potato wilt; root rot of lilies, peonies, gerberas, *Cymbidium*, calendula, and calendula wilt; and root rot of *Atractylodes macrocephala*, *Salvia miltiorrhiza*, and *Fern rhizome*. This includes diseases affecting medicinal herbs such as root rot, American ginseng root rot, American ginseng wilt, Polygonatum sibiricum root rot, Codonopsis pilosula root rot, castor bean root rot, Atractylodes lancea root rot, Dendrobium officinale root rot, Sophora tonkinensis root rot, Rehmannia glutinosa root rot, Aconitum carmichaelii root rot, Scutellaria baicalensis root rot, Coptis chinensis root rot, Ligusticum striatum root rot, Panax notoginseng root rot, Angelica sinensis root rot, Ligusticum chuanxiong root rot, Saposhnikovia divaricata root rot, Saposhnikovia divaricata wilt, Rheum palmatum root rot, Clematis armandii root rot, Areca catechu root rot, etc.; sugarcane root rot, watermelon root rot, watermelon wilt. Strawberry wilt, melon wilt, melon Fusarium fruit rot, blueberry root rot, banana root rot, banana wilt and other fruit crop diseases; tomato fruit rot, tomato Fusarium root rot, tomato wilt, eggplant root rot, eggplant wilt, pepper root rot, pepper wilt, cucumber root rot, bottle gourd wilt, loofah wilt, bitter gourd root rot, bitter gourd wilt, Buddha's hand wilt, pumpkin wilt, winter melon wilt, green bean root rot, green bean wilt, cowpea root rot, cowpea wilt, pea Diseases of vegetable crops such as root rot, pea wilt, broad bean root rot, broad bean wilt, celery root rot, garlic dry rot, garlic root rot, mung bean root rot, zucchini root rot, snow pea root rot, onion root rot, Sichuan pepper root rot, carrot root rot, moso bamboo root rot, and ginger root rot; diseases of oil crops such as sesame wilt, peanut root rot, and soybean root rot; and other crop diseases such as cotton wilt, flax wilt, tobacco Fusarium root rot, tobacco Fusarium wilt, and licorice root rot.

[0033] The preferred soil-borne fungal diseases are selected from:

[0034] Fusarium infection causes soil-borne fungal crop diseases, the diseases are selected from the group consisting of oat root rot, wheat root rot, corn root rot, barley root rot, potato dry rot, potato root rot, potato wilt, sweet potato root rot, sweet potato wilt and other food crop diseases; lily root rot, peony root rot, gerbera root rot, gerbera wilt, calendula root rot, calendula wilt and other flower crop diseases; root rot of white atractylodes rhizome, root rot of salvia miltiorrhiza, root rot of salvia miltiorrhiza, root rot of potentilla discolor, root rot of panax quinquefolium, root rot of panax quinquefolium, root rot of polygonatum multiflorum, root rot of radix codonopsis, root rot of ricinus communis, root rot of atractylodes lancea, root rot of dendrobium officinale, root rot of radix sophorae tonkinensis, root rot of rehmannia glutinosa, root rot of aconitum, root rot of eucommia ulmoides, root rot of coptis chinensis, root rot of ligusticum sinense, root rot of panax notoginseng, root rot of angelica sinensis, root rot of ligusticum chuanxiong, root rot of saposhnikovia divaricata, root rot of radix et rhizoma rhei, root rot of caryopteris mongholica, root rot of areca catechu and other Chinese herbal medicine crop diseases; sugarcane root rot, watermelon root rot, watermelon wilt, strawberry wilt, melon wilt, melon fusarium fruit rot, blueberry root rot, banana root rot, banana wilt and other fruit crop diseases; tomato fruit rot, tomato fusarium root rot, tomato wilt, eggplant root rot, eggplant wilt, pepper root rot, pepper wilt, cucumber root rot, bottle gourd wilt, luffa wilt, balsam pear root rot, balsam pear wilt, bergamot wilt, pumpkin wilt, winter melon wilt, bean root rot, bean wilt, cowpea root rot, cowpea wilt, pea root rot, pea wilt, broad bean root rot, broad bean wilt, parsley root rot, garlic dry rot, garlic root rot, mung bean root rot, zucchini root rot, Dutch pea root rot, onion root rot, prickly ash root rot, carrot root rot, bamboo root rot, ginger root rot and other vegetable crop diseases; sesame wilt, peanut root rot, soybean root rot and other oil crop diseases; cotton wilt, flax wilt, tobacco fusarium root rot, tobacco fusarium wilt, licorice root rot and other crop diseases.

[0035] The soil treatment composition provided by the application can prevent and control crop nematode diseases, and the diseases are selected from the group consisting of:

[0036] Crop nematode diseases caused by Aphelenchoides infection, and the diseases are selected from the group consisting of narcissus aphelenchoides disease, gerbera aphelenchoides disease, tulip aphelenchoides disease, begonia aphelenchoides disease, rhododendron aphelenchoides disease and peony aphelenchoides disease and other flower crop diseases.

[0037] Crop nematode diseases caused by Ditylenchus infection, and the diseases are selected from the group consisting of narcissus ditylenchus disease, tulip ditylenchus disease, lily ditylenchus disease, chrysanthemum ditylenchus disease and other flower crop diseases; sweet potato ditylenchus disease and other food crop diseases.

[0038] Crop nematode diseases caused by Heterodera infection, the diseases are selected from soybean cyst nematode disease and other oil crops diseases.

[0039] Crop nematode diseases caused by Meloidogyne infection, the diseases are selected from tomato root-knot nematode disease, eggplant root-knot nematode disease, pepper root-knot nematode disease, cucumber root-knot nematode disease, beet root nematode disease, luffa root-knot nematode disease, bitter gourd root-knot nematode disease, zucchini root-knot nematode disease, kidney bean root-knot nematode disease, cowpea root-knot nematode disease, lettuce root-knot nematode disease, radish root-knot nematode disease, Chinese cabbage root-knot nematode disease, water spinach root-knot nematode disease, spinach root-knot nematode disease, celery root-knot nematode disease, ginger root-knot nematode disease and other vegetable crops diseases; watermelon root-knot nematode disease, melon root-knot nematode disease and other fruit crops diseases.

[0040] Preferably, the crop nematode diseases are selected from:

[0041] Crop nematode diseases caused by Meloidogyne infection, the diseases are selected from tomato root-knot nematode disease, eggplant root-knot nematode disease, pepper root-knot nematode disease, cucumber root-knot nematode disease, beet root nematode disease, luffa root-knot nematode disease, bitter gourd root-knot nematode disease, zucchini root-knot nematode disease, kidney bean root-knot nematode disease, cowpea root-knot nematode disease, lettuce root-knot nematode disease, radish root-knot nematode disease, Chinese cabbage root-knot nematode disease, water spinach root-knot nematode disease, spinach root-knot nematode disease, celery root-knot nematode disease, ginger root-knot nematode disease and other vegetable crops diseases; watermelon root-knot nematode disease, melon root-knot nematode disease and other fruit crops diseases.

[0042] Further, the soil treatment composition provided by the present application can be used for preventing and treating crops, including: food, flowers, Chinese herbal medicines, fruits, vegetables, oil plants and the like.

[0043] The food crops are selected from oat, wheat, barley, rice, corn and other cereal food crops, and also include sweet potato, potato, yam, konjac and other tuberous food crops.

[0044] The flower crops are selected from woody flower crops such as peony, clove, michelia, white orchid, jasmine, winter jasmine and the like; herbaceous flower crops such as peony, hyacinth, tulip, heliptropium, marigold, African violet, chrysanthemum, narcissus and the like; pot flower crops such as Chinese weigela, carnation, chrysanthemum, cymbidium, aloe, hyacinth and the like; fresh-cut flower crops such as carnation, Chinese rose, skyblue and the like.

[0045] The Chinese herbal medicine crops are selected from atractylodes, salvia miltiorrhiza, potentilla discolor, panax quinquefolium, polygonatum, radix codonopsis, ricinus communis, atractylodes lancea, dendrobium officinale, eurycoma longifolia, rehmannia glutinosa, aconitum, euodia, coptis, ligusticum wallichii, panax notoginseng, angelica sinensis, ligusticum wallichii, saposhnikovia divaricata, radix et rhizoma rhei, berberis dictyophylla, piper nigrum.

[0046] The fruit crops are selected from the group consisting of pome fruit crops such as apples, pears, papayas, etc.; stone fruit crops such as peaches, apricots, plums, prunes, etc.; berry fruit crops such as figs, kiwis, etc.; nut fruit crops such as walnuts, Japanese nut pine, etc.; miscellaneous fruit crops such as dates, persimmons, pomegranates, etc.; citrus fruit crops such as citrus, oranges, lemons, etc.; perennial herbaceous fruit crops such as bananas, pineapples, strawberries, etc.; and other fruit crops such as watermelons, melons, etc.

[0047] The vegetable crops are selected from the group consisting of root vegetables such as radishes, daikon, turnips, etc.; allium vegetables such as green onions, onions, garlic, etc.; Chinese cabbage vegetables such as Chinese cabbage, bok choy, pickled mustard, etc.; cabbage vegetables such as cabbage, purple cabbage, cauliflower, etc.; leafy vegetables such as spinach, Chinese kale, amaranth, celery, etc.; solanaceous vegetables such as tomatoes, eggplants, peppers, etc.; melon vegetables such as cucumbers, bottle gourds, loofahs, balsams, zucchinis, squashes, pumpkins, wax gourds, etc.; legume vegetables such as snap beans, cowpeas, broad beans, peas, etc.; and perennial vegetable crops such as lettuce, Chinese toon, day lily, etc.

[0048] The oil crops are selected from the group consisting of sesame, peanuts, soybeans, sunflowers, etc.

[0049] The other crops are selected from the group consisting of cotton, flax, licorice, tobacco, etc.

[0050] The soil treatment composition according to the present application can be applied to plants, plant propagation materials, and subsequently grown plant organs, cultivation media, materials, or spaces by seed treatment, drenching, dripping, pouring, dusting, or spreading.

[0051] The skilled person knows that, in the field of soil treatment, referring to the disclosed technologies such as Cotton Soil Disinfection Technical Regulations Q / WSSQ001-2023, combined with the actual situation in the field, the effective ingredient use amount of DBCP and metam is 20-40 kg / acre and 17.5-35 kg / acre respectively, and both require film mulching fumigation for 15-30 days. During this period, DBCP and metam are degraded into methyl isothiocyanate to play a fumigation role, which can effectively kill nematodes, fungi and bacterial harmful organisms by inhibiting cell division and the synthesis of DNA, RNA and protein and causing respiratory obstruction, so as to obtain clean and healthy soil. After fumigation is completed, the film is removed and exposed to air for 7-15 days to fully release methyl isothiocyanate and avoid drug damage to crops. In addition, the effect of soil fumigation is also affected by factors such as soil conditions, temperature, soil moisture, distribution of the agent and the integrity of the film. In summary, the fumigation method and product characteristics determine that methyl isothiocyanate compounds basically have no effect on harmful organisms after the film is removed. Harmful organisms can easily infect the soil again through soil, seed, seedling and substrate transmission methods. Fluopyram, as an internal absorbent agent, does not have fumigation performance, and is not recommended for use in soil treatment due to its high cost.

[0052] The present application combines methyl isothiocyanate compounds and fluopyram in a specific ratio and uses them for soil treatment. It is unexpectedly found that the combination has a good synergistic effect on the prevention and control of nematodes and soil-borne fungal diseases. In soil treatment, the use amount of DBCP and metam can be significantly reduced, and the control effect can be improved. The use amount of DBCP and metam used alone is large, but after being combined with fluopyram and prepared into a suitable formulation product, it can be more evenly distributed in the soil, reducing the use amount while also effectively controlling the nematode and disease base. Further, the present application composition can further prolong the prevention and control time, and the soil control effect of the composition can be prolonged to 90 days. From the end of fumigation to the early stage of crop growth, the spread of nematodes and soil-borne fungal diseases in the soil is effectively prevented and controlled. In addition, the present application composition can further expand the target of the prevention and control object. While having a long duration, the fluopyram in the present application composition can enter the plant body in the early stage of crop growth while preventing soil harmful organisms, effectively preventing nematode and fungal disease infection, preventing seed-borne diseases and some leaf diseases, such as mainly air-borne alternaria leaf spot and powdery mildew, thereby prolonging the protection period and better protecting crop growth.

[0053] Compared with the prior art, the present application has the following advantages:

[0054] 1. Cotton / DBCP and metam have a significant synergistic effect when combined with fluopyram, and the control effect on pathogens is significantly better than that of a single agent.

[0055] 2. The action mechanism and mode of triforine / propamocarb and fluopyram are different, and the mixture can delay the generation of target drug resistance.

[0056] 3. The mixture of triforine / propamocarb and fluopyram reduces the amount of pesticide used, lowers the use cost and reduces the pollution to the environment, and meets the development requirements of pesticide reduction and efficiency increase.

[0057] 4. The soil treatment agent scheme disclosed in the application can consider soil treatment and disease prevention in the early growth stage of crops, and has good economic and social benefits. DETAILED DESCRIPTION

[0058] It should be understood that the specific embodiments described herein are intended to be illustrative only and not limiting of the present application, with any modifications, equivalent replacements and improvements made within the spirit and principle of the present application being included in the protection scope of the present application. In these embodiments, all percentages are weight percentages unless otherwise specified.

[0059] Preparation excipient examples

[0060] Excipient Example 1: 40% triforine·fluopyram wettable powder (799:1)

[0061] 1.1 Formulation design: 39.95% triforine, 0.05% fluopyram, 2.0% NNO, 3.0% PICO-JS20, 2.0% 704, kaolin (carrier) to 100%.

[0062] 1.2 Preparation method: triforine, fluopyram, NNO, PICO-JS20, 704 and kaolin are put into a mixing kettle and mixed for 10 minutes, and the above materials are airflow pulverized to D90≤25μm to obtain the finished product.

[0063] 1.3 Test data: the indicators of the product meet the index requirements of conventional wettable powder.

[0064] Example 2: 40% propamocarb·fluopyram aqueous solution (666:1)

[0065] 2.1 Formulation design: 39.94% propamocarb, 0.06% fluopyram, 1.0% dimethyl decanamide, 0.6% 505, 0.8% 601, deionized water (carrier) to 100%.

[0066] 2.2 Preparation method: fluopyram is dissolved with dimethyl decanamide, 505 and 601 are added and stirred uniformly, propamocarb is added and stirred uniformly, and finally deionized water is added to make up the finished product.

[0067] 2.3 Test data: the indicators of the product meet the index requirements of aqueous solution.

[0068] Example 3: 20% dinitramine · fluopyram dispersible oil suspension (999:1)

[0069] 3.1 Formulation design: 19.98% dinitramine, 0.02% fluopyram, 3.0% Tween-80, 5.0% Y-70B, 3.0% AEO-7, 1.0% bentonite, methyl oleate (carrier) to 100%.

[0070] 3.2 Preparation method: First, put methyl oleate, Tween-80, Y-70B, AEO-7 and magnesium aluminum silicate into the stirring kettle, stir uniformly, then add dinitramine and fluopyram, stir and shear until there are no visible chunks in the kettle, start the sand mill for grinding treatment, and when the particle size D90≤5μm, discharge the finished product.

[0071] 3.3 Test data: The indicators of the product meet the requirements of conventional dispersible oil suspensions.

[0072] Example 4: 90% dinitramine · fluopyram water dispersible granules (899:1)

[0073] 4.1 Formulation design: 89.9% dinitramine, 0.1% fluopyram, 3.0% D-425, 2.0% UNA, 0.5% EFW, 1.5% PVP k30, corn starch (carrier) to 100%.

[0074] 4.2 Preparation method: Put dinitramine, fluopyram, D-425, UNA, EFW and kaolin into the mixing kettle and mix for 10 minutes, then airflow crush the above materials to D90≤25μm, mix the crushed materials with deionized water in which PVP k30 has been previously dissolved (160-180kg of deionized water per ton of material) using a mixer to make the material plastic, extrude the uniformly mixed material into cylindrical particles with a diameter of 1.0mm and dry with 90℃ hot air until the moisture content is less than 3%, and the finished product is obtained.

[0075] 4.3 Test data: The indicators of the product meet the requirements of conventional dispersible granules.

[0076] Example 5: 20% metam potassium · fluopyram emulsion in water (799:1)

[0077] 5.1 Formulation design: 19.975% metam potassium, 0.025% fluopyram, 1.0% S-200, 0.6% 505, 0.2% 601, 1.2% BY-110, deionized water (carrier) to 100%.

[0078] 5.2 Preparation method: Dissolve fluazinam in S-200, then add 505, 601 and BY-110 and stir to obtain an oil phase, stir flumioxazin and deionized water to obtain an aqueous phase, slowly shear the oil phase into the aqueous phase, and stir uniformly to obtain the finished product.

[0079] 5.3 Detection data: The indicators of the product meet the requirements of conventional emulsion in water.

[0080] Example 6: 40% flumioxazin·fluazinam suspension concentrate (519:1)

[0081] 6.1 Formula design: 39.923% flumioxazin, 0.077% fluazinam, 0.2% FLK, 0.1% KT-50, 0.3% SAG-1522, 0.5% magnesium aluminum silicate, 0.1% xanthan gum, deionized water (carrier) to 100%.

[0082] 6.2 Preparation method: Shear and stir deionized water, FLK, KT-50, magnesium aluminum silicate and SAG-1522 uniformly, then add fluazinam and flumioxazin and shear again until there are no large chunks of raw materials in the material, grind to D90≤5μm with a sand mill, and after discharging, shear uniformly with 2% xanthan gum water solution prepared in advance to obtain the finished product.

[0083] 6.3 Detection data: The indicators of the product meet the requirements of conventional suspension concentrate.

[0084] Example 7: 97% dazomet·fluazinam granules (1209:1)

[0085] 7.1 Formula design: 96.92% dazomet, 0.08% fluazinam, 0.004% polyvinyl alcohol 1788, 0.03% FLK, 0.02% KT-50, deionized water, white carbon black (carrier) to 100%.

[0086] 7.2 Preparation method: Shear and stir deionized water, 0.3% FLK and 0.2% KT-50 uniformly, add 0.8% fluazinam and shear again until there are no large chunks of raw materials in the material, grind to D90≤5μm with a sand mill, and after discharging, stir uniformly with 0.8% polyvinyl alcohol 1788 stock solution (5% aqueous solution prepared in advance by hot water for 24h) to obtain 0.8% fluazinam stock solution for standby. Put dazomet and white carbon black into a mixing kettle and mix for 10 minutes, then perform air jet milling on the above-mentioned materials until D90≤25μm, and discharge to obtain dazomet solid powder. Spray the fluazinam stock solution uniformly on the dazomet solid powder according to the proportion, and dry to obtain the finished product.

[0087] 7.3 Detection data: The indicators of the product meet the requirements of conventional granules.

[0088] Field efficacy implementation

[0089] Field control test:

[0090] The complex form of the composition of the present application involved in the field test is carried out by single-agent tank mixing, and the single-agent tank mixing is selected from 98% thiabendazole microgranules (Shunyi Co., Ltd.), 41.7% fluopyram suspension concentrate (Bayer CropScience (China) Co., Ltd.), and 41% metam sodium aqueous solution (Limin Chemical Co., Ltd.) unless otherwise specified.

[0091] Test one: field control test of thiabendazole and fluopyram on fusarium

[0092] The overall design of the test is based on part of the test design in [Yang Jianzhong, et al. Continuous cropping soil treatment for the control effect of panax ginseng root rot [J]. Special product research, 2010, (2): 37-39] and [Ma Chengzhe, et al. Panax ginseng continuous cropping field root rot complex syndrome comprehensive control measures and effect [J]. Shanghai Journal of Agricultural Sciences, 2006, 22(4): 63-68]; the method of application is based on part of the test design in 《Thiabendazole soil disinfection technical procedures Q / WSSQ 001-2023》; the quantitative analysis method of fusarium is based on the detection method in [Ge Bo, et al. Fusarium composition and quantitative analysis in corn rhizosphere soil [J]. Chinese Journal of Agricultural Sciences, 2018, (19): 3683-3693].

[0093] Test purpose: field control effect of different proportions of composition on fusarium.

[0094] The control target is fusarium (Fusarium).

[0095] Control crop: panax ginseng.

[0096] Test agent: 98% thiabendazole microgranules (Shunyi Co., Ltd.), 41.7% fluopyram suspension concentrate (Bayer CropScience (China) Co., Ltd.).

[0097] Test design (1-year-old panax ginseng): the plots are arranged in random order, and are sown by point planting with row spacing and plant spacing of 6×6 cm, with a plot area of 2.0 m×2.0 m, about 1111 plants per plot, and a planting density of 277 plants / m 2 , and the specified amount of drug is given.

[0098] Test investigation method: take soil with a depth of 25 cm to detect fusarium content 1 day before application and 30, 50, 70 and 90 days after application.

[0099] Experimental method: 40 days before sowing, the soil was first tilled to a depth of 30-40 cm, then the experimental agent was diluted with water and sprayed, and tilled again to ensure it was evenly mixed into the 30-40 cm soil layer, and then covered with plastic film. 25 days later, the film was removed, the soil was loosened, and residual toxic gases were discharged. 15 days later, fumigation was completed.

[0100] Example of field efficacy trial: (Wenshan County, Wenshan Prefecture, Yunnan Province - Wenshan Panax notoginseng Industrial Park)

[0101] Planting area: The total planting area of ​​Panax notoginseng in the experimental area is close to 1,200 mu.

[0102] Soil conditions: Fields with severe root rot disease that have been continuously cropped with Panax notoginseng for more than 6 years, with clayey, slightly acidic, red soil and medium to high fertility.

[0103] Small-scale trial: According to the experimental design, soil samples were collected on November 2, 2021 (30 days before sowing) before application of the fungicide. Soil samples were taken at the planned sowing sites 15 and 30 days after application to test for Fusarium content. Rhizosphere soil samples were taken from Panax notoginseng at 50, 70, and 90 days after application to test for Fusarium content. No other chemical fungicides were used in the experimental fields during the trial period, and management practices remained the same as usual.

[0104] Calculation formula:

[0105]

[0106] Theoretical efficacy (%) = [1 - (1 - C1) × (1 - C2)] × 100%

[0107] Synergistic effect coefficient of mixed formulation:

[0108] In the formula, C1 and C2 represent the actual efficacy of a single agent; SR≤0.8 indicates antagonistic effect, 0.8<SR<1.2 indicates additive effect, and SR≥1.2 indicates synergistic effect.

[0109] Table 1: Field efficacy trial design of dazomet and fluopyram against Fusarium spp.

[0110]

[0111]

[0112] Table 2: Field control efficacy of dazomet and fluopyram against Fusarium spp.

[0113]

[0114]

[0115] Experimental conclusion:

[0116] 1) When the ratio of dazomet and fluopyram is in the range of 570:1-1210:1, the synergistic effect is manifested as addition, then enhancement, and then addition again, and no antagonism occurs. When the ratio is in the range of 650:1-1050:1 (treatments 4, 7, 10, 13, 16 and 19), the synergistic effect on Fusarium fungi is better, and the effect is most obvious at the ratio of 730:1, 810:1 and 890:1 (treatments 7, 10, 13), which is 1.34, 1.42 and 1.36, respectively;

[0117] 2) Compared with dazomet alone, the combination of dazomet and fluopyram significantly reduces the decay rate of sterilization effect and prolongs the duration of sterilization performance. For example, the effect of the combination at the ratio of 810:1 (treatment 10) on Fusarium is 41.71% at 90 days after treatment, while the effect of dazomet alone (treatment 10) is only 20.24%; the effect of the combination at the ratio of 890:1 (treatment 13) on Fusarium is 41.89% at 90 days after treatment, while the effect of dazomet alone (treatment 11) is only 22.30%. This phenomenon shows that the combination of dazomet and fluopyram can prolong the duration of effect within a certain ratio range.

[0118] Test two: Verify whether the effect of the soil treatment composition of the application after reducing the amount of application can reach the effect of the full amount of single agent.

[0119] Test description: The combination ratio with the most significant synergistic effect in test one is selected for the effect verification of reducing the amount of application in the second year, so this test is a supplementary test of test one, and the test method is the same as that of test one.

[0120] Table 3: Test design of the field effect of the combination on Fusarium after reducing the amount of application

[0121]

[0122]

[0123] Table 4: Test results of the field effect of the combination on Fusarium after reducing the amount of application

[0124]

[0125] Test conclusion: 1) From the effects at 15 days, 30 days and 50 days after treatment, the effect of reducing the amount of application by 10% is better than that of the full amount of dazomet single agent, and the effect of reducing the amount of application by 15% is still comparable to that of the full amount of dazomet single agent, which shows that the combination of dazomet and fluopyram can reduce the amount of application appropriately while still meeting the basic effect requirements.

[0126] During the experiment, it was observed that some Panax notoginseng seedlings were infected with Alternaria panax and powdery mildew in the non-test area, and a small amount of Panax notoginseng seedlings were infected in the test area treated with single-dose of DCDM or single-dose of fluazamam, but no infection was found in the test area treated with the composition of the present application, thereby proving that the composition of the present application can treat some stem and leaf diseases, thereby better protecting the growth of crops.

[0127] Test three: field control effect of DCDM and fluazamam on tomato root-knot nematode

[0128] The overall design of the test is based on [Zhang Bohu, Zhang Feng, Wen Yajun, Yang Xiaofeng, Jia Kaifeng, Meng Yan, Zhu Xuerong, Hao Pingqi. Control effect of two nematicides on root-knot nematode in watermelon field. Heilongjiang Agricultural Science, 2017(7): 35-37.]; the application method is based on part of the test design of “DCDM soil disinfection technical procedures Q / WSSQ 001-2023”.

[0129] Test purpose: field control effect of different proportions of the composition on tomato root-knot nematode.

[0130] Control target: Meloidogyne.

[0131] Control crop: tomato.

[0132] Test method: the application method is the same as test one, and the tomato seedlings are transplanted 30 days after application.

[0133] Investigation time: investigation is conducted before application and when tomato root-knot nematode disease is symptomatic.

[0134] Investigation method: 10 plants are randomly investigated in each plot by “five-point sampling method”, and the occurrence of nematodes in the roots is investigated.

[0135] Root-knot nematode disease grading standard:

[0136] Table 7 Root-knot nematode disease grading standard

[0137]

[0138]

[0139] Calculation method:

[0140]

[0141]

[0142] Among them, the synergistic coefficient calculation method is the same as test one.

[0143] Field control effect test example: (Taian Township, Lijiang City, Yunnan Province)

[0144] Planting area: The total area of tomato planting in the test field was close to 40 mu.

[0145] Soil conditions: Sandy soil, loose and well-drained.

[0146] Disease incidence: Root-knot nematode disease occurs year-round in this area and can occur throughout the tomato growth period.

[0147] Test of prevention effect: According to the test design, the drug was given on June 20, 2022, and the film was opened on July 10, 2022. Tomato seedlings were transplanted on July 20, 2022. The incidence and survival rate were investigated 10-30 days after transplanting. No other fungicides were used in the test field during the test period, and the management measures were the same as usual.

[0148] Test results:

[0149] Table 8 Field test results of control effect of dazomet and fluopyram on root-knot nematode

[0150]

[0151]

[0152] Test conclusion: When the ratio of dazomet and fluopyram is in the range of 570:1-1210:1, the synergistic effect is first additive, then synergistic, and then additive, and no antagonism occurs. When the ratio is 650:1-1130:1 (treatments 4, 7, 10, 13, 16, 19, and 22), the control of root-knot nematode has a good synergistic effect. The three ratios of 810:1, 890:1, and 970:1 (treatments 10, 13, and 16) are the most obvious, with synergistic ratios of 1.36, 1.34, and 1.30, respectively.

[0153] Test four: Pot test of dazomet and fluopyram on tomato root-knot nematode

[0154] The overall design of the test is based on [Liu Chunyan, Wang Wanli, Hou Jianfei, Hao Yongjuan, Wang Yong. Field test of 98% dazomet microgranules for controlling cucumber root-knot nematode. North Horticulture, 2011(23): 128-130.]; The method of application is based on part of the test design of 《Dazomet soil disinfection technical regulations Q / WSSQ 001-2023》.

[0155] Test explanation: The four ratios with the most significant synergistic effect in test three were selected to verify the persistence period, so this test is a supplementary test of test three.

[0156] Control target: Root-knot nematode (Meloidogyne).

[0157] Control crop: Tomato.

[0158] Test method: The application method is the same as test 1, and the tomato seedlings are transplanted at 30d, 45d, 60d and 90d after application.

[0159] Investigation time: before application and when tomato root-knot nematode disease is manifested.

[0160] Investigation method: 1000g of soil sample is taken from each plot by "five-point sampling method", mixed thoroughly, and then 200g of soil sample is taken to separate nematodes in the soil by modified Berthelot funnel method. After killing nematodes by heat method, the number of 2nd instar larvae is counted under a stereoscopic dissecting microscope.

[0161] Pot experiment prevention effect test example is the same as test 3.

[0162] Calculation method:

[0163]

[0164] Test design:

[0165] Table 9: Test design of dazomet and fluopyram on root-knot nematode in pot experiment

[0166]

[0167] Test results:

[0168] Table 10: Test results of dazomet and fluopyram on root-knot nematode in pot experiment

[0169]

[0170] Test conclusion: The reduction rate of insect population in dazomet single agent treatment group decreases with the extension of time after application, the reduction rate of insect population is 92.76% at 30d after application, and only 77.13% at 90d after application, but the reduction rate of insect population in dazomet and fluopyram combination treatment group is relatively stable, and there is no attenuation phenomenon in the reduction rate of insect population, which indicates that dazomet combined with fluopyram can prolong the effective period.

[0171] Test 5: Field prevention effect test of dimethomorph and fluopyram on Fusarium

[0172] Test explanation: Except that 41% dimethomorph aqueous solution (Limin Chemical Co., Ltd.) is used, the application amount and composition ratio are changed, and other test conditions are the same as test 1.

[0173] Table 11: Test design of dimethomorph and fluopyram on Fusarium in field

[0174]

[0175] Table 12: Test results of dimethomorph and fluopyram on Fusarium in field

[0176]

[0177]

[0178] Test Conclusion:

[0179] 1) The synergistic effect of the combination of metam and fluopyram is additive at first, then synergistic, and then additive again, and no antagonism is observed when the ratio is in the range of 460:1 to 940:1. When the ratio is in the range of 520:1 to 820:1 (treatments 4, 7, 10, 13, 16, 19), the combination has a good synergistic effect on the control of Fusarium fungi, and the effect is most obvious at ratios of 640:1, 700:1 and 760:1 (treatments 10, 13, 16), which are 1.37, 1.29 and 1.26, respectively.

[0180] 2) The combination of metam and fluopyram reduces the decay rate of sterilization performance and prolongs the duration of sterilization effect compared with metam alone. For example, the control effect of the combination at a ratio of 640:1 (treatment 10) on Fusarium is 40.70% at 90 days after treatment, while the control effect of metam alone (treatment 11) is only 18.57%. The control effect of the combination at a ratio of 700:1 (treatment 13) on Fusarium is 36.00% at 90 days after treatment, while the control effect of metam alone (treatment 14) is only 15.73%. This phenomenon indicates that the combination of metam and fluopyram can prolong the duration of effect within a certain ratio range.

[0181] Test Six: Verification of whether the control effect of the soil treatment composition of the present application after reduced dosage can reach the control effect of full dosage.

[0182] Test Explanation: The combination with the most significant synergistic effect in Test Five is selected for the control effect verification of reduced dosage in the second year, so this test is a supplementary test of Test Five, and the test method is the same as that of Test Five.

[0183] Table 13: Test design of field control effect of Fusarium after reduced dosage of the composition

[0184]

[0185] Table 14: Test results of field control effect of Fusarium after reduced dosage of the composition

[0186]

[0187] Test Conclusion: When the dosage is reduced by 10%, the control effect at each node is better than that of full dosage of metam alone, which indicates that the combination of metam and fluopyram can reduce the dosage appropriately while still meeting the basic control requirements.

[0188] During the rotation test, it was observed that some Panax notoginseng seedlings were infected with Alternaria panax and Sphaerotheca fuliginea in the non-test area, and some Panax notoginseng seedlings were infected with Alternaria panax and Sphaerotheca fuliginea in the single-dose cotton or single-dose dimethomorph treatment area of the test area, but no infection was found in the composition treatment area of the test area, thus it is inferred that the composition described in the application can treat some crop stem and leaf diseases, thereby better protecting the growth of crops.

[0189] Test seven: field control effect test of dimethomorph and fluopyram on tomato root-knot nematode

[0190] Except that the test agent is 41% dimethomorph aqueous solution (Limin Chemical Co., Ltd.) and the application amount and composition ratio are changed, the other test conditions are the same as those in test three

[0191] Test results:

[0192] Table 15: Field control effect test results of dimethomorph and fluopyram

[0193]

[0194] Test conclusion: when the combination of dimethomorph and fluopyram is used in the ratio of 460:1 to 940:1, the synergistic effect is first additive, then synergistic, and then additive, and no antagonism occurs. When the ratio is 520:1 to 820:1 (treatment numbers 4, 7, 10, 13, 16, and 19), the control of root-knot nematode has a good synergistic effect, and the ratio of 640:1, 700:1, and 760:1 (treatment numbers 10, 13, and 16) is the most obvious, with synergistic ratios of 1.32, 1.31, and 1.26, respectively.

[0195] Test eight: pot control effect test of dimethomorph and fluopyram on tomato root-knot nematode

[0196] Test explanation: four combinations with significant synergistic effect in test seven are selected to verify the persistence period, so this test is a supplementary test of test seven, and the test method is the same as that of test four.

[0197] Test design:

[0198] Table 16: Pot control effect test design of dimethomorph and fluopyram

[0199]

[0200] Test results:

[0201] Table 17: Pot control effect test results of dimethomorph and fluopyram

[0202]

[0203] The test conclusion is that the insect population reduction rate of the single treatment group of dimethipin decreases with the extension of time after treatment, the insect population reduction rate is 82.59% on the 30th day after treatment, and only 73.83% on the 90th day after treatment, but the insect population reduction rate of the dimethipin and fluopyram compound treatment group is relatively stable, and the insect population reduction rate basically has no attenuation phenomenon, which shows that the dimethipin compound fluopyram can prolong the persistence period.

[0204] In summary, the dimethipin or fluazuron compound fluopyram has significant synergistic effect in a specific proportion range, and appropriate reduction of the use amount of active ingredients can achieve the effect of sufficient single agent use; by virtue of the long persistence period of fluopyram, the dimethipin or fluazuron compound fluopyram can significantly prolong the persistence period; by virtue of the systemicity and broad-spectrum characteristics of fluopyram, the dimethipin or fluazuron compound fluopyram can also control part of the mild stem and leaf diseases in the process of prevention and treatment, thereby better protecting the growth of crops.

Claims

1. A soil treatment composition, characterized in that, The composition comprises a methyl isothiocyanate compound and fluopyram, wherein the methyl isothiocyanate compound is dazomet or thiamethoxam, wherein when the methyl isothiocyanate compound is dazomet, the weight ratio of dazomet to fluopyram is 650:1 to 1050:1, and the total weight of the active ingredients accounts for 20%-95% of the weight of the composition; when the methyl isothiocyanate compound is thiamethoxam, the weight ratio of thiamethoxam to fluopyram is 520:1 to 820:1, and the total weight of the active ingredients accounts for 20%-60% of the weight of the composition.

2. The soil treatment composition according to claim 1, characterized in that, The soil treatment composition can be formulated with appropriate excipients into agriculturally usable formulations, such as emulsions, suspensions, dispersible oil suspensions, water-dispersible granules, pellets, tablets, and suspension emulsions.

3. The use of the soil treatment composition according to claim 1 in the prevention and control of fungal diseases and nematode damage in crops, characterized in that, The fungal disease of the crop is a soil-borne fungal disease, and the pathogen is selected from the genus Fusarium (Fusarium). Fusarium The pathogenic nematodes were selected from the genus *Root-knot Nematodes*. Meloidogyne ).

4. The use according to claim 3, characterized in that, The soil treatment composition is applied to the plant cultivation medium or space in an agronomically effective and substantially non-phytotoxic amount by seed treatment, soaking, dripping, watering or dispersing methods.

Images