A stem cell culture medium sampling and filtering system and sampling and processing method

Abstract

The present invention discloses a stem cell culture medium sampling and filtering system and a sampling and processing method, comprising a filtering component for raw protein liquid sampling, a pipeline component for raw protein liquid in and out of the filtering component, and a supporting component for installing and positioning the filtering component, wherein the filtering component and the pipeline component constitute a stem cell culture medium sampling and filtering system for raw protein liquid. The filtering component is added to filter solid impurities mixed in the raw protein liquid, ensure the standardization of the sample of the raw protein liquid, and further improve the accuracy of the raw protein liquid sampling and detection results. At the same time, a plurality of the blades and the annular filter screen are combined to form an impeller to filter the solids in the raw protein liquid. At the same time, the impeller, driven by a motor, has an attraction effect, that is, filtering while accelerating the sampling process, and improving the flow rate of the raw protein liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of oil sampling, and more specifically, to a stem cell culture medium sampling and filtering system and a sampling and processing method. Background Art

[0002] Generally speaking, the basic components of stem cell culture medium can be divided into the following categories: basal culture medium, buffer system, glutamine, serum (or serum substitutes), specific growth factors and other supplements.

[0003] Classic basal culture media (DMEM, MEM, RPMI, etc.) are formulas with clear chemical compositions. Each formula is a basic culture material environment matrix developed to support specific cell lines or culture conditions. However, in addition to meeting the nutritional needs of cultured cells, stem cell culture media must also pay attention to other culture conditions of the cells.

[0004] For example, the pH value for the growth of most mammalian cells is around 7.4, so it is also important to maintain a relatively stable pH environment.

[0005] Therefore, when manufacturing stem cell culture medium, consideration is given to adding carbon dioxide to achieve the technical purpose of enhancing acidity and lowering the pH value in the culture medium.

[0006] Another example is serum-free culture media, which allows researchers to culture stem cells without serum.

[0007] The main advantage of serum-free stem cell culture media is consistent and reproducible experimental results from a purer media formulation.

[0008] Generally speaking, serum-free culture medium is considered to be a culture medium with defined ingredients, containing albumin, insulin, selenium and other proteins, etc. However, further studies have found that in the process of manufacturing serum-free culture medium, common culture medium raw protein liquid often contains large particles of animal fat (or fat particles). For example, the raw protein liquid extracted from terrestrial warm-blooded animals and poultry contains animal fat that is not needed by the culture medium, so it is necessary to take samples and directly mix them into serum-free culture medium, which can easily affect the experimental results of cell culture.

[0009] It is especially important to achieve small-dose extraction efficiency when extracting raw protein liquid at the laboratory level; when sampling and testing the raw protein liquid, a sampling device is required, and the existing sampling device cannot implement the filtering function when sampling. When the existing sampling device samples liquid animal fats, most sampling devices cannot filter out impurities in the liquid animal fats, so it is easy to take away the impurity fats in the raw protein liquid, which in turn affects the subsequent cell culture experimental results. Therefore, it is necessary to design a filtration system that can filter the impurity fats in the raw protein liquid for practical development and use. Summary of the invention

[0010] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a stem cell culture medium sampling and filtering system and a sampling and processing method, so as to solve the problems raised in the above-mentioned background technology.

[0011] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a stem cell culture medium sampling and filtering system and a sampling and processing method, comprising a filtering component for sampling a raw protein liquid, a pipeline component for the raw protein liquid in and out of the filtering component, and a supporting component for installing and positioning the filtering component, wherein the filtering component and the pipeline component constitute a stem cell culture medium sampling and filtering system for the raw protein liquid;

[0012] The filter assembly comprises two sampling cylinders placed vertically side by side, a cylinder cover arranged at the inner edge of the opening of the sampling cylinder, a motor fixed at the middle position of the outer side of the cylinder cover, and an annular filter connected to the output shaft of the motor, blades are evenly distributed on the circumference of the annular filter, and any blade is arranged at the same angle with the annular filter, and a plurality of blades and the annular filter are combined to form an impeller;

[0013] The pipeline assembly includes an upper conveying pipe arranged on the peripheral side of the sampling barrel, a lower conveying pipe arranged in the middle position of the bottom side of the sampling barrel, a sampling tube connected to the middle of the upper conveying pipe, and a discharge conduit connected to the middle of the lower conveying pipe, the upper conveying pipe is vertically connected to the peripheral side of the sampling barrel, and the lower conveying pipe is arranged in a colinear manner with the peripheral axis of the sampling barrel, the sampling tube is inclined upward relative to the upper conveying pipe, and a spherical end is arranged at the end of the sampling tube, and the peripheral side of the spherical end is hollowed out, the discharge conduit for discharging the raw protein liquid is inclined downward relative to the lower conveying pipe, and the discharge conduit matches the external raw protein liquid collection mechanism;

[0014] The filtering assembly structure composed of the annular filter and the sampling barrel is used to accelerate the raw protein liquid in the space between the annular filter and the sampling barrel to enter the annular filter to form a spiral vortex filtering process under the action of the impeller formed by the combination of the blades and the annular filter driven by the motor;

[0015] It also includes a secondary spiral filtering mechanism; the secondary spiral filtering mechanism is used to perform a secondary spiral vortex filtering treatment operation on the raw protein liquid before the sampling tube flows through the lower conveying pipe.

[0016] In a preferred embodiment, the support assembly includes a bracket arranged outside the two sampling tubes and an arc-shaped balance block arranged inside the vertical side of the bracket, and the arc-shaped surface of any arc-shaped balance block is in contact with the circumference of the sampling tube, and the arc-shaped balance block performs vibration balance on the sampling tube through a built-in damper structure.

[0017] In a preferred embodiment, the secondary spiral filter mechanism comprises a first sleeve and a second sleeve; the first sleeve comprises a sleeve body and a turning edge arranged at the top of the sleeve body and arranged outward along the outer edge, the diameter of the turning edge is larger than the diameter of the sleeve body of the first sleeve; a grid support frame is arranged inside the turning edge; a head bearing is arranged at the center of the grid support frame, and a passive shaft is mounted on the head bearing; the first filter plate and the second filter plate are fixedly connected to the interior of the sleeve body from top to bottom along the passive shaft;

[0018] The first filter plate and the second filter plate each include a base plate and a shaft sleeve arranged at a substantially central position, and the shaft sleeves of the first filter plate and the second filter plate are rotationally matched with the passive rotating shaft;

[0019] The surface of the first filter plate and the surface of the second filter plate are both inclined downward with respect to the passive rotating shaft, and a first notched screen is provided at the outermost edge of the downwardly inclined surface of the first filter plate. Similarly, a second notched screen is provided at the outermost edge of the downwardly inclined surface of the second filter plate. The first notched screen and the second notched screen are arranged opposite to each other, one at the leftmost side and the other at the rightmost side relative to the passive rotating shaft.

[0020] In a preferred embodiment, the first notched screen and the second notched screen are both fan-shaped notched screens.

[0021] In a preferred embodiment, the annular filter is disposed inside the sampling barrel, and the output shaft of a motor for driving the annular filter to rotate passes through the barrel cover.

[0022] In a preferred embodiment, a control valve for controlling the flow of the raw protein liquid is provided in the middle of the lower conveying pipe and the upper conveying pipe.

[0023] In a preferred embodiment, ear seats are equidistantly fixed on the circumference of the sampling barrel, the ear seats are movably connected to a limit bolt, the limit bolt is threadedly engaged with a limit knob, and the limit bolt matches the notched screen provided on the edge of the barrel cover, and the barrel cover remains fixed in position due to the interference between the limit knob and the opening edge of the sampling barrel.

[0024] In a preferred embodiment, a conical depression is provided in the middle of the bottom of the sampling cylinder, and the raw protein liquid inside the sampling cylinder forms a vortex with the conical depression as the center.

[0025] In a preferred embodiment, a liquid level switch is fixed at two-thirds of the height of the inner wall of the sampling cylinder. When the liquid level inside the sampling cylinder reaches the liquid level switch, the liquid level switch triggers the motor to continue operating.

[0026] Accordingly, the present invention also provides a sampling and processing method for a stem cell culture medium sampling and filtering system, which is applicable to the above-mentioned stem cell culture medium sampling and filtering system, and the steps of the method are as follows:

[0027] S1: fixing the support on a horizontal plane to determine the operating position of the stem cell culture medium sampling and filtering system;

[0028] S2: connecting the sampling tube to the raw protein liquid storage mechanism, and connecting the discharge conduit to the external raw protein liquid collection mechanism;

[0029] S3: Open the control valve, so that the raw protein liquid enters the sampling cylinder along the sampling tube and the upper conveying tube under the action of gravity. When the liquid level of the raw protein liquid in the sampling cylinder reaches two-thirds of the height relative to the sampling cylinder, the liquid level switch is triggered. At the same time, the motor is started, and the output shaft of the motor drives the annular filter to rotate, further causing the blades fixed on the periphery of the annular filter to rotate synchronously. Under the action of the impeller formed by the combination of the blades and the annular filter, the raw protein liquid in the space between the annular filter and the sampling cylinder is accelerated to enter the annular filter.

[0030] S4: The raw protein liquid filtered by the annular filter enters the secondary spiral filtering mechanism before entering the lower conveying pipe to perform secondary spiral vortex filtering treatment operation, and then is discharged through the lower conveying pipe and the discharge conduit, and is collected by the external raw protein liquid collecting mechanism, completing the filtering sampling of the raw protein liquid.

[0031] Technical effects and advantages of the present invention:

[0032] In the first aspect, the stem cell culture medium sampling and filtering system provided by the embodiment of the present invention filters solid impurities mixed in the raw protein liquid by adding a filtering component in the sampling system, thereby ensuring the standardization of the sampling samples of the raw protein liquid and further improving the accuracy of the raw protein liquid sampling and detection results; in order to improve the purity of the raw protein liquid in a targeted manner, the concept of secondary spiral filtration theory is proposed, and the details are shown in the subsequent technical content.

[0033] In the second aspect, the present invention achieves the fixation of the cylinder cover by using the cooperation of the limit bolt and the limit knob, further fixes the position of the motor and the combination of the plurality of blades and the annular filter to form the impeller, and the convenient disassembly of the cylinder cover will make the combination of the plurality of blades and the annular filter inside the sampling cylinder to form the impeller simple and convenient in the subsequent cleaning and maintenance process;

[0034] Thirdly, the present invention combines a plurality of blades and an annular filter to form an impeller to filter the solids in the raw protein liquid. At the same time, the impeller, driven by a motor, has a suction effect, that is, it filters while speeding up the sampling process and increasing the flow rate of the raw protein liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of one side of the overall structure of the present invention;

[0036] Figure 2 It is a schematic diagram of the other side of the overall structure of the present invention;

[0037] Figure 3 It is a side schematic diagram of the overall structure of the present invention;

[0038] Figure 4 It is a schematic diagram of the structure of the filter assembly in the present invention;

[0039] Figure 5 It is a cross-sectional view of the internal structure of the sampling tube in the present invention;

[0040] Figure 6 It is a schematic diagram of the installation of the annular filter screen and the blades in the present invention;

[0041] Figure 7 It is a schematic diagram of the installation of the cylinder cover and the sampling cylinder in the present invention;

[0042] Figure 8 It is a schematic diagram of the overall assembly of the two-stage spiral filtering mechanism of the present invention;

[0043] Fig. 9 It is a schematic diagram from another perspective of an overall assembly of the two-stage spiral filtering mechanism of the present invention;

[0044] Fig.10It is a partial side assembly schematic diagram of the secondary spiral filtering mechanism of the present invention after the partial structure is hidden;

[0045] Fig.11 It is a partial three-dimensional assembly schematic diagram of the secondary spiral filtering mechanism of the present invention after hiding part of the structure;

[0046] Fig.12 A schematic diagram of a partial explosion structure of the secondary spiral filtering mechanism of the present invention;

[0047] Fig.13 A schematic diagram of an experimental comparison effect between the stem cell culture medium sampling and filtration system provided by an embodiment of the present invention and an existing filtration device.

[0048] The accompanying drawings are marked as follows:

[0049] 1. Filter assembly; 11. Sampling tube; 12. Tube cover; 13. Motor; 14. Annular filter; 15. Blade; 16. Ear seat; 17. Limit bolt; 18. Limit knob; 19. Liquid level switch; 2. Pipe assembly; 21. Upper conveying pipe; 22. Lower conveying pipe; 23. Sampling tube; 24. Discharge conduit; 25. Spherical end; 26. Control valve; 3. Support assembly; 31. Bracket; 32. Arc balance block; 33. Mounting block; 34. Lifting ring; 4. Secondary spiral filter mechanism; 41. First sleeve; 42. Second sleeve; 411. Sleeve body; 412. Turning edge; 413. Grid support frame; 414. Head bearing; 415. Passive shaft; 43. First filter plate; 44. Second filter plate; 431. Base plate; 432. Bushing; 433. First notch screen; 441. Second notch screen. DETAILED DESCRIPTION

[0050] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] Embodiment 1

[0052] like Figure 1-7 , Embodiment 1 of the present invention provides a stem cell culture medium sampling and filtering system, comprising a filtering component 1 for sampling a raw protein liquid, a pipeline component 2 for the raw protein liquid in and out of the filtering component 1, and a supporting component 3 for installing and positioning the filtering component 1; the filtering component 1 and the pipeline component 2 constitute a stem cell culture medium sampling and filtering system for the raw protein liquid;

[0053] The filter assembly 1 includes two sampling cylinders 11 placed vertically side by side, a cylinder cover 12 arranged at the inner edge of the opening of the sampling cylinder 11, a motor 13 fixed at the middle position outside the cylinder cover 12, and an annular filter screen 14 connected to the output shaft of the motor 13;

[0054] The annular filter screen 14 is evenly distributed with blades 15 on the circumference, and any blade 15 is set at the same angle as the annular filter screen 14; a plurality of blades 15 and the annular filter screen 14 are combined to form an impeller; the annular filter screen 14 is arranged inside the sampling barrel 11, and the output shaft of the motor 13 for driving the annular filter screen 14 to rotate passes through the barrel cover 12; the bottom middle position of the sampling barrel 11 is provided with a conical depression, and the raw protein liquid inside the sampling barrel 11 forms a vortex with the conical depression as the center (implementing a spiral filtering operation);

[0055] The filtering assembly structure composed of the annular filter 14 and the sampling tube 11 is used to accelerate the raw protein liquid in the space between the annular filter 14 and the sampling tube 11 to enter the annular filter 14 to form a spiral vortex filtering process under the driving action of the motor 13 so that the blades 15 and the annular filter 14 form an impeller.

[0056] The pipeline assembly 2 includes an upper delivery pipe 21 disposed on the periphery of the sampling cylinder 11, a lower delivery pipe 22 disposed in the middle of the bottom side of the sampling cylinder 11, a sampling pipe 23 connected to the middle of the upper delivery pipe 21, and a discharge conduit 24 connected to the middle of the lower delivery pipe 22;

[0057] A control valve 26 for controlling the flow of the raw protein liquid is disposed in the middle of the lower delivery pipe 22 and the upper delivery pipe 21. The upper delivery pipe 21 is vertically connected to the peripheral side of the sampling tube 11, and the lower delivery pipe 22 is collinearly arranged with the peripheral axis of the sampling tube 11. The sampling tube 23 is inclined upward relative to the upper delivery pipe 21, and a spherical end 25 is disposed at the end of the sampling tube 23. The peripheral side of the spherical end 25 is hollowed out. The discharge conduit 24 for discharging the raw protein liquid is inclined downward relative to the lower delivery pipe 22, and the discharge conduit 24 matches the external raw protein liquid collection mechanism.

[0058] It also includes a secondary spiral filtering mechanism 4 ; the secondary spiral filtering mechanism 4 is used to perform a secondary spiral vortex filtering treatment operation on the raw protein liquid before the sampling tube 11 flows through the lower conveying pipe 22 .

[0059] The stem cell culture medium sampling and filtering system involved in the first embodiment of the present application is mainly used for sampling raw protein liquid. In order to avoid fat particles or impurities mixed in the sampled liquid, a filtering component 1 is added to remove larger fat particles. When the liquid level of the raw protein liquid inside the sampling cylinder 11 reaches two-thirds of the height relative to the sampling cylinder 11, the liquid level switch 19 is triggered. At the same time, the motor 13 is started, and the output shaft of the motor 13 drives the annular filter 14 to rotate, further causing the blades 15 fixed on the periphery of the annular filter 14 to rotate synchronously. Under the action of the impeller formed by the combination of the blades 15 and the annular filter 14, the raw protein liquid in the space between the annular filter 14 and the sampling cylinder 11 is accelerated to enter the annular filter 14.

[0060] Further explanation of the above structure is as follows: when the motor 13 is started, the blades 15 and the annular filter screen 14 are combined to form an impeller to extract the raw protein liquid in the space between the annular filter screen 14 and the sampling tube 11 into the inside, and then the raw protein liquid enters the lower conveying pipe 22 from the annular filter screen 14 under its own gravity or external suction, and is finally discharged from the lower conveying pipe 22 and forms a vortex at the conical depression at the bottom of the sampling tube 11. The two flush-arranged sampling tubes 11 not only speed up the sampling and filtering efficiency; but also perform a secondary eddy current filtering process before entering the lower conveying pipe 22; the structure of the secondary spiral filtering mechanism is detailed in Figure 8-Figure 12 The secondary spiral filtering mechanism 4 is used to perform secondary spiral vortex filtering treatment operation on the raw protein liquid before the sampling cylinder 11 flows through the lower conveying pipe 22; at the same time, when a single sampling cylinder 11 needs to be cleaned and maintained, the other sampling cylinder 11 can also be filtered, so as to achieve the purpose of continuous operation of the stem cell culture medium sampling and filtering system.

[0061] It should be explained that the pipeline assembly 2 is used for conveying the raw protein liquid flowing through the filter assembly 1 in and out. In actual use, the sampling tube 23 is connected to the raw protein liquid storage mechanism, and the discharge conduit 24 is connected to the external raw protein liquid collection mechanism. The control valve 26 is opened, so that the raw protein liquid enters the sampling cylinder 11 along the sampling tube 23 and the upper conveying tube 21 under the action of gravity. The raw protein liquid filtered by the filter assembly 1 is discharged through the lower conveying tube 22 and the discharge conduit 24, and is collected by the external raw protein liquid collection mechanism, thereby completing the filtration sampling of the raw protein liquid.

[0062] Among them, the sampling tube 23 and the discharge conduit 24 are both inclined, which is used for smoother circulation of the raw protein liquid and makes full use of its own gravity to flow. When facing the raw protein liquid with a higher density, its own fluidity is poor. At this time, it is necessary to install an extraction pump at the discharge conduit 24 to assist the circulation of the raw protein liquid in the pipeline component 2. The extended end of the spherical end 25 set at the end of the sampling tube 23 is threadedly connected to the inside of the sampling tube 23; at the same time, the setting of the spherical end 25 is used for the initial filtration of the raw protein liquid, mainly for the separation of larger volume of oil solids, to avoid larger particles of oil fixed in the pipeline component 2 and transported to cause pipeline blockage, and granular oil solids may also cause blockage of the hollow area of ​​the spherical end 25; at this time, you can choose to directly clean the hollow area of ​​the spherical end 25, or you can increase the suction at the discharge conduit 24, and then divide the oil solids into small particles under the dividing action of the hollow edge of the spherical end 25, so that the pipeline component 2 cannot be blocked.

[0063] Embodiment 2

[0064] In the second embodiment, the support assembly 3 includes a bracket 31 arranged outside the two sampling tubes 11 and an arc-shaped balance block 32 arranged inside the vertical side of the bracket 31. The arc surface of any arc-shaped balance block 32 is in contact with the peripheral side of the sampling tube 11, and the arc-shaped balance block 32 performs vibration balance on the sampling tube 11 through a built-in damper structure; a mounting block 33 extends from the bottom of the bracket 31, and a lifting ring 34 is fixed at the top corner of the bracket 31. The mounting block 33 is fixed to the contact surface through a bolt structure, and the lifting ring 34 is used for connecting an external lifting mechanism;

[0065] It should be noted that the support assembly 3 is used for installing and positioning the sampling barrel 11. In actual use, the installation sequence between the bracket 31 and the contact surface and between the bracket 31 and the sampling barrel 11 is not in particular order and is not subject to any restrictive constraints. Here, the bracket 31 and the sampling barrel 11 are preferentially installed as an example, and the bottom of the sampling barrel 11 is fixed to the bottom of the bracket 31, and the peripheral side of the sampling barrel 11 is fitted with the arc surface of the arc balance block 32 fixed on the inner side of the bracket 31;

[0066] The damper built into the arc-shaped balance block 32 reduces the vibration of the sampling tube 11 in the form of energy conversion consumption, which is a relatively conventional vibration reduction measure. The lifting ring 34 is moved by the lifting mechanism to move the bracket 31 to the working position, and the mounting block 33 is fixed to the contact surface, so that the overall position of the bracket 31 is fixed; the bottom support feet of the bracket 31 are raised to facilitate the installation and use of the subsequent discharge conduit 24. With the above structure, the installation and positioning of the sampling tube 11 is realized, and the normal operation of the stem cell culture medium sampling and filtration system is ensured.

[0067] Embodiment 3

[0068] Refer to the instruction manual Figure 1-7 , Embodiment 3 of the present invention provides a stem cell culture medium sampling and filtering system, comprising a filtering component 1 for sampling a raw protein liquid, a pipeline component 2 for the raw protein liquid in and out of the filtering component 1, and a supporting component 3 for installing and positioning the filtering component 1; the filtering component 1 and the pipeline component 2 constitute a stem cell culture medium sampling and filtering system for the raw protein liquid;

[0069] The filter assembly 1 includes two sampling cylinders 11 placed vertically side by side, a cylinder cover 12 arranged at the inner edge of the opening of the sampling cylinder 11, a motor 13 fixed at the middle position outside the cylinder cover 12, and an annular filter screen 14 connected to the output shaft of the motor 13;

[0070] The annular filter screen 14 is evenly distributed with blades 15 on the circumference, and any blade 15 is set at the same angle as the annular filter screen 14; a plurality of blades 15 and the annular filter screen 14 are combined to form an impeller; the annular filter screen 14 is arranged inside the sampling barrel 11, and the output shaft of the motor 13 for driving the annular filter screen 14 to rotate passes through the barrel cover 12; the bottom middle position of the sampling barrel 11 is provided with a conical depression, and the raw protein liquid inside the sampling barrel 11 forms a vortex with the conical depression as the center (implementing a spiral filtering operation);

[0071] The stem cell culture medium sampling and filtering system involved in the present application is provided with a filtering component 1 to remove larger particles in order to prevent excessive oil particles or impurities in the sampled liquid; wherein, when the liquid level of the raw protein liquid inside the sampling cylinder 11 reaches two-thirds of the height relative to the sampling cylinder 11, the liquid level switch 19 is triggered; at the same time, the motor 13 is started, and the output shaft of the motor 13 drives the annular filter 14 to rotate, further causing the blades 15 fixed on the periphery of the annular filter 14 to rotate synchronously, and under the action of the impeller formed by the combination of the blades 15 and the annular filter 14, the raw protein liquid in the space between the annular filter 14 and the sampling cylinder 11 is accelerated to enter the annular filter 14;

[0072] The third embodiment of the present application also provides a secondary spiral filtering mechanism, whose main function is to implement secondary spiral filtering treatment. The secondary spiral filtering mechanism is installed inside the first connecting pipe between the bottom of the sampling cylinder 11 and the lower conveying pipe 22, that is, at the connecting pipe position at the front end of the control valve 26; the above-mentioned secondary spiral filtering mechanism is a passive spiral driving mechanism. When the raw protein liquid enters the lower conveying pipe 22 from the annular filter screen 14 of the sampling cylinder 11 under the action of its own gravity and the downward thrust of the spiral or the action of external suction, it first enters the above-mentioned first connecting pipe, and the inside of the above-mentioned first connecting pipe is provided with a secondary spiral filtering mechanism;

[0073] like Figure 8-Figure 12 The secondary spiral filtering mechanism 4 comprises a first sleeve 41 and a second sleeve 42; the first sleeve 41 comprises a sleeve body 411 and a turning edge 412 arranged on the top of the sleeve body along the outer edge outward, the diameter of the turning edge 412 is larger than the diameter of the sleeve body 411 of the first sleeve 41 (the turning edge is similar to the structure of the inner sleeve plug, so that it is installed and sleeved on the inner wall of the sleeve body); a grid support frame 413 (a plurality of support frame structures arranged crisscrossly in the extension direction of the sleeve body) is arranged inside the turning edge; a head bearing 414 is arranged at the center of the grid support frame, and a passive rotating shaft 415 is installed on the head bearing (that is, the passive rotating shaft is not installed with any driving motor for cooperation, but rotates passively and autonomously); the first filter plate 43 and the second filter plate 44 are fixedly connected to the interior of the sleeve body 411 from top to bottom along the passive rotating shaft;

[0074] The first filter plate 43 and the second filter plate 44 have similar structures, both comprising a base plate 431 and a shaft sleeve 432 arranged at a substantially central position, and the shaft sleeves of the first filter plate and the second filter plate are rotationally matched with the passive rotating shaft, and the extension direction of the shaft sleeves on the first filter plate and the second filter plate is not perpendicular to the base plate but is inclined;

[0075] The surface of the first filter plate and the surface of the second filter plate are both in a downwardly inclined state with respect to the passive rotating shaft, the surface of the first filter plate 43 and the surface of the second filter plate 44 are in an eight-shaped shape, and a first notched screen 433 (preferably a fan-shaped notched screen) is provided at the outermost edge of the surface of the first filter plate 43 extending downwardly in an inclined manner, and similarly, a second notched screen 441 is provided at the outermost edge of the surface of the second filter plate 44 extending downwardly in an inclined manner; at the same time, the projections of the first notched screen and the second notched screen in the same direction along the passive rotating shaft do not overlap (a preferred embodiment is that the first notched screen and the second notched screen are arranged oppositely, one on the left side and the other on the right side relative to the passive rotating shaft), so that it can be ensured that the liquid flowing out of the first notched screen 433 changes its flow direction after entering the second filter plate and then flows out from the second notched screen 441 of the second filter plate, and in this process, a secondary spiral vortex of the raw protein liquid is realized, and a secondary filtration process of high-speed flow is realized in this process;

[0076] At the same time, the inner wall surface of the sleeve body and the filter plates (the first filter plate and the second filter plate) are both smooth surfaces, so as to ensure that the filter plates can rotate freely;

[0077] To sum up, the two-stage spiral filtration mechanism provided by the embodiment of the present invention, wherein the first filter plate and the second filter plate respectively have a notched sieve structure, that is, the notched sieve structure is a component structure formed by installing a sieve in a fan-shaped notch, which can achieve fine pore filtration processing while ensuring the passage of vortex vortex, thereby further reducing impurities in the original protein liquid.

[0078] The second sleeve is smaller in size in order to abut against the inner wall of the control valve 26 at its rear end. The grounding effect can help the secondary spiral filter mechanism resist the impact of high-speed eddy currents and avoid damage to the first sleeve on the secondary spiral filter mechanism and the relatively fragile filter plate and other structures on the inner wall.

[0079] Embodiment 4

[0080] In this embodiment, ear seats 16 are fixed equidistantly around the sampling tube 11, and the ear seats 16 are movably connected to limit pins 17, and the limit pins 17 are threadedly matched with limit knobs 18, and the limit pins 17 match the notches set on the edge of the tube cover 12, and the tube cover 12 is kept in a fixed position under the interference of the limit knob 18 and the opening edge of the sampling tube 11;

[0081] It should be noted that when in use, the stepped edge of the barrel cover 12 is buckled into the inner edge of the opening of the sampling barrel 11, and the notch arranged on the circumference of the barrel cover 12 is aligned with the limit bolt 17. Under the action of the limit bolt 17, the horizontal position of the barrel cover 12 is limited and cannot rotate horizontally with the sampling barrel 11. Subsequently, the lower limit knob 18 is threadedly installed on the end of the limit bolt 17, and the limit knob 18 is rotated clockwise until the limit knob 18 conflicts with the barrel cover 12. At this time, the barrel cover 12 maintains a fixed relative position with the sampling barrel 11 under the horizontal limit of the limit bolt 17 and the vertical limit of the limit knob 18. The relative position of the barrel cover 12 and the sampling barrel 11 is fixed by the above structure, and its loading and unloading is more convenient without affecting the stability.

[0082] Embodiment 5

[0083] Based on the same principle, the fifth embodiment of the present invention also provides a sampling and processing method for a stem cell culture medium sampling and filtering system. The method is applicable to the above-mentioned stem cell culture medium sampling and filtering system. The steps of the method are as follows:

[0084] S1: Fix the support 31 on a horizontal plane to determine the operating position of the stem cell culture medium sampling and filtering system;

[0085] S2: connecting the sampling tube 23 to the raw protein liquid storage mechanism, and connecting the discharge conduit 24 to the external raw protein liquid collection mechanism;

[0086] S3: Open the control valve 26, so that the raw protein liquid enters the sampling cylinder 11 along the sampling tube 23 and the upper conveying tube 21 under the action of gravity. When the liquid level of the raw protein liquid in the sampling cylinder 11 reaches two-thirds of the height relative to the sampling cylinder 11, the liquid level switch 19 is triggered. At the same time, the motor 13 is started, and the output shaft of the motor 13 drives the annular filter 14 to rotate, further causing the blades 15 fixed on the periphery of the annular filter 14 to rotate synchronously. Under the action of the impeller formed by the combination of the blades 15 and the annular filter 14, the raw protein liquid in the space between the annular filter 14 and the sampling cylinder 11 is accelerated to enter the annular filter 14;

[0087] S4: The raw protein liquid filtered by the annular filter 14 enters the secondary spiral filtering mechanism to perform secondary spiral vortex filtering treatment operation before entering the lower conveying pipe, and then is discharged through the lower conveying pipe and the discharge conduit, and is collected by the external raw protein liquid collecting mechanism, completing the filtering sampling of the raw protein liquid.

[0088] The sampling and processing method of the above-mentioned stem cell culture medium sampling and filtration system provided in the embodiment of the present invention can separate and filter and block some oil particles through high-speed eddy current motion through secondary eddy current filtration, thereby improving the purity of the raw protein liquid and ensuring the stability of the culture medium.

[0089] In order to verify the use of the sampling and processing method of the stem cell culture medium sampling and filtering system provided in Example 5 of the present invention, the following experiment was designed, as shown in the experimental control data chart (see Fig.13 ), used to illustrate the different filtering effects of three groups, namely, direct extraction without filtration, single filtration extraction by disassembling the secondary spiral filtration mechanism of the stem cell culture medium sampling and filtration system of the present application, and filtration extraction implemented by the complete stem cell culture medium sampling and filtration system of the present application, on the filtration of oil particles from the raw protein liquid from the same source.

[0090] Based on the above content, please provide three sets of control data to verify the data comparison table of culture medium purity obtained by no filtration, single filtration and double filtration for oil particles; the first group is to select samples 1-10, that is, direct extraction without filtration. The second group selects samples 11-20, that is, single filtration extraction after disassembling the secondary spiral filtration mechanism; the third group selects samples 21-30, that is, single filtration extraction after disassembling the secondary spiral filtration mechanism.

[0091] The above three groups of control data show the effects of no filtration, single filtration and double filtration on the purity of culture medium obtained from oil particles. By comparing the culture medium purity data under different filtration methods, the difference in the removal effect of oil particles by different filtration methods can be evaluated.

[0092] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, which may refer to mechanical connection or electrical connection, or internal communication between two components, or direct connection. "upper", "lower", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may change;

[0093] Secondly: In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other;

[0094] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A stem cell culture medium sampling and filtering system, comprising a filtering component (1) for sampling a raw protein liquid, a pipeline component (2) for the raw protein liquid in and out of the filtering component (1), and a supporting component (3) for mounting and positioning the filtering component (1), wherein the filtering component (1) and the pipeline component (2) constitute a stem cell culture medium sampling and filtering system for the raw protein liquid, characterized in that: The filter assembly (1) comprises two sampling cylinders (11) placed vertically side by side, a cylinder cover (12) arranged at the inner edge of the opening of the sampling cylinder (11), a motor (13) fixed at the middle position outside the cylinder cover (12), and an annular filter (14) connected to the output shaft of the motor (13), blades (15) are evenly distributed on the circumference of the annular filter (14), any blade (15) and the annular filter (14) are arranged at the same angle, and a plurality of blades (15) and the annular filter (14) are combined to form an impeller; The pipeline assembly (2) comprises an upper conveying pipe (21) arranged on the circumference of the sampling cylinder (11), a lower conveying pipe (22) arranged at the middle position of the bottom side of the sampling cylinder (11), a sampling pipe (23) connected to the middle part of the upper conveying pipe (21), and a discharge conduit (24) connected to the middle part of the lower conveying pipe (22); the upper conveying pipe (21) is vertically connected to the circumference of the sampling cylinder (11), and the lower conveying pipe (22) and the circumference axis of the sampling cylinder (11) are arranged in a colinear manner; the sampling pipe (23) is inclined upward relative to the upper conveying pipe (21), and a spherical end cap (25) is arranged at the end of the sampling pipe (23), and the circumference of the spherical end cap (25) is hollowed out; the discharge conduit (24) for discharging the raw protein liquid is inclined downward relative to the lower conveying pipe (22), and the discharge conduit (24) matches an external raw protein liquid collection mechanism; The filtering assembly structure composed of the annular filter (14) and the sampling tube (11) is used to accelerate the raw protein liquid in the space between the annular filter (14) and the sampling tube (11) to enter the annular filter (14) to form a spiral vortex filtering treatment operation under the driving action of the motor (13) so that the blades (15) and the annular filter (14) are combined to form an impeller; It also includes a secondary spiral filtering mechanism; the secondary spiral filtering mechanism is used to perform a secondary spiral vortex filtering treatment operation on the raw protein liquid before the sampling cylinder (11) flows through the lower conveying pipe (22); The secondary spiral filtering mechanism (4) comprises a first sleeve (41) and a second sleeve (42); the first sleeve comprises a sleeve body (411) and a turning edge (412) arranged at the top of the sleeve body and arranged outward along the outer edge, the diameter of the turning edge (412) being larger than the diameter of the sleeve body (411); a grid support frame (413) is arranged inside the turning edge; a head bearing (414) is arranged at the center of the grid support frame (413), and a passive rotating shaft (415) is mounted on the head bearing; a first filter plate (43) and a second filter plate (44) are fixedly connected to the interior of the sleeve body from top to bottom along the passive rotating shaft; The first filter plate and the second filter plate both comprise a base plate (431) and a shaft sleeve (432) arranged at a substantially central position, and the shaft sleeves of the first filter plate and the second filter plate are rotationally matched with the passive rotating shaft; The surface of the first filter plate and the surface of the second filter plate are both in a downwardly inclined state relative to the passive rotating shaft, and a first notched screen (433) is provided at the outermost edge of the downwardly inclined surface of the first filter plate, and similarly, a second notched screen (441) is provided at the outermost edge of the downwardly inclined surface of the second filter plate; the first notched screen and the second notched screen are arranged opposite to each other, one at the leftmost side and the other at the rightmost side relative to the passive rotating shaft.

2. The stem cell culture medium sampling and filtering system according to claim 1, characterized in that: The support assembly (3) comprises a bracket (31) arranged outside the two sampling barrels (11) and an arc-shaped balance block (32) arranged inside the vertical side of the bracket (31), wherein the arc-shaped surface of any arc-shaped balance block (32) is in contact with the peripheral side of the sampling barrel (11), and the arc-shaped balance block (32) performs vibration balance on the sampling barrel (11) through a built-in damper structure.

3. The stem cell culture medium sampling and filtering system according to claim 2, characterized in that: The first notched screen and the second notched screen are both fan-shaped notched screens.

4. The stem cell culture medium sampling and filtering system according to claim 3, characterized in that: The annular filter (14) is arranged inside the sampling barrel (11), and an output shaft of a motor (13) for driving the annular filter (14) to rotate passes through the barrel cover (12).

5. The stem cell culture medium sampling and filtering system according to claim 4, characterized in that: A control valve (26) for controlling the flow of the raw protein liquid is provided in the middle of the lower delivery pipe (22) and the upper delivery pipe (21).

6. The stem cell culture medium sampling and filtering system according to claim 5, characterized in that: A liquid level switch (19) is fixed at two-thirds of the height of the inner wall of the sampling cylinder (11). When the liquid level inside the sampling cylinder (11) reaches the liquid level switch (19), the liquid level switch (19) triggers the motor (13) to continue operating.

7. The stem cell culture medium sampling and filtering system according to claim 6, characterized in that: The middle position of the bottom of the sampling tube (11) is provided with a conical depression, and the raw protein liquid inside the sampling tube (11) forms a vortex with the conical depression as the center.

8. The stem cell culture medium sampling and filtering system according to claim 7, characterized in that: Ear seats (16) are fixed at equal intervals on the circumference of the sampling tube (11); the ear seats (16) are movably connected to a limit pin (17); the limit pin (17) is threadedly engaged with a limit knob (18); and the limit pin (17) matches a notch provided on the edge of the tube cover (12); the tube cover (12) is kept in a fixed position under the interference between the limit knob (18) and the opening edge of the sampling tube (11).

9. A sampling and processing method for a stem cell culture medium sampling and filtering system, the method being applicable to the stem cell culture medium sampling and filtering system according to claim 8, the method comprising the following steps: S1: fixing the support (31) on a horizontal plane to determine the operating position of the stem cell culture medium sampling and filtering system; S2: connecting the sampling tube (23) to the raw protein liquid storage mechanism, and connecting the discharge conduit (24) to the external raw protein liquid collection mechanism; S3: opening the control valve (26) so that the raw protein liquid flows into the sampling cylinder (11) along the sampling tube (23) and the upper conveying tube (21) under the action of gravity. When the liquid level of the raw protein liquid in the sampling cylinder (11) reaches two-thirds of the height relative to the sampling cylinder (11), the liquid level switch (19) is triggered. At the same time, the motor (13) is started, and the output shaft of the motor (13) drives the annular filter (14) to rotate, further causing the blades (15) fixed on the circumference of the annular filter (14) to rotate synchronously. Under the action of the impeller formed by the combination of the blades (15) and the annular filter (14), the raw protein liquid in the space between the annular filter (14) and the sampling cylinder (11) is accelerated to enter the annular filter (14). S4: The raw protein liquid filtered by the annular filter (14) enters a secondary spiral filtering mechanism to perform a secondary spiral vortex filtering treatment operation before entering the lower conveying pipe, and then is discharged through the lower conveying pipe (22) and the discharge conduit (24) and collected by an external raw protein liquid collecting mechanism, thereby completing the filtration sampling of the raw protein liquid.

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