A synergistic multi-dimensional anti-wrinkle skin care system and methods of making and using the same

Abstract

The present application discloses a synergistic multi-dimensional anti-wrinkle skincare system, its preparation method and use method. The system comprises four complementary formula combinations. Combination 1 combines specific moisturizing agents with plant and biological fermentation extracts, aiming to optimize the water content of the stratum corneum and maintain skin health. Combination 2 uses active ingredients such as hydroxypropyl tetrahydropyran triol to activate cell renewal and enhance dermal elasticity. Combination 3 is designed specifically for eye wrinkles, using high-efficiency peptides and active substances. Combination 4 combines antioxidant, moisturizing, and wrinkle-removing functions to combat wrinkles. The system relies on scientific proportioning technology and precise process flow to ensure that each combination produces a synergistic effect. From improving moisture retention, strengthening the skin barrier, stabilizing the basement membrane structure, to promoting collagen synthesis, the system provides a comprehensive and systematic skincare solution to effectively combat wrinkles caused by skin aging.

Description

Technical Field

[0001] This invention relates to the fields of cosmetic technology and bioengineering, and in particular to an innovative skincare solution designed to address wrinkles caused by skin aging. The invention primarily involves developing four customized compositions and combining them with specific preparation processes to comprehensively improve and prevent skin aging from multiple levels. Background Technology

[0002] The cosmetics industry has made significant progress in anti-aging skincare, especially in preventing and treating wrinkles. Existing patents on the market often focus on single or a few anti-wrinkle mechanisms, such as improving the stratum corneum's moisture content and barrier function by adding moisturizing ingredients (e.g., hyaluronic acid, glycerin), or using bioactive peptides (e.g., pentapeptides, hexapeptides) to promote collagen synthesis and enhance skin elasticity. However, these existing anti-wrinkle solutions often overlook the complexity of wrinkle formation and fail to comprehensively address key factors in the aging process at all levels of the skin, including free radical scavenging, glycation inhibition, optimization of cell renewal rate, and maintenance of dermal fibroblast activity.

[0003] For example, existing patented skincare products containing Pro-Xylane (e.g., at a concentration of 20%) primarily improve skin structural integrity by stimulating fibroblast proliferation and basement membrane repair, but their targeting of dynamic wrinkles and eye wrinkles may be insufficient. Furthermore, some patented inventions utilize plant extracts combined with anti-glycation and antioxidant systems, which can effectively combat skin aging caused by environmental stress, but further improvements are needed to achieve synergistic anti-aging effects on both the epidermis and dermis and enhance overall skin health.

[0004] In view of this, the present invention aims to provide a more systematic and comprehensive anti-wrinkle skincare solution, designing four customized compositions to combat the various causes of wrinkles from different dimensions. The first composition integrates highly effective moisturizing ingredients and antioxidant and anti-glycation plant extracts, not only increasing the moisture content of the stratum corneum but also focusing on maintaining the skin's healthy microecological balance. The second composition uses a complex formula with high concentrations of Pro-Xylane and Bifida Ferment Lysate to deeply intervene in the regenerative capacity of the dermis while strengthening the basement membrane support structure. The third composition is specifically designed for the characteristics of the skin around the eyes, combining signal peptides and highly effective anti-wrinkle agents to solve the unique static and dynamic wrinkles around the eyes. Finally, the fourth composition comprehensively utilizes a variety of innovative moisturizing and repairing ingredients to consolidate and amplify the anti-wrinkle effect of the entire system, thereby achieving comprehensive and deep anti-aging skincare.

[0005] Therefore, the technical background of this invention is to fill the gap in the multi-dimensional synergistic effect of existing anti-wrinkle skin care products. By scientifically and rationally formulating various active ingredients, a skin care product system that comprehensively covers all aspects of skin aging is constructed to meet the increasingly diversified market demands and consumers' pursuit of efficient, safe, and long-lasting anti-wrinkle products. Summary of the Invention

[0006] The technical problem solved by this invention is to provide a synergistic multi-dimensional anti-wrinkle skin care product system, its preparation method and application method. This system provides a comprehensive and effective solution for various wrinkle problems caused by skin aging through four carefully designed complementary compositions (compositions 1-4).

[0007] Specific technical challenges include, but are not limited to: how to effectively increase the moisture content of the stratum corneum and maintain a healthy skin condition to delay the formation of fine lines and wrinkles caused by epidermal moisture loss; how to activate and promote skin cell renewal and enhance the elasticity of the dermis to reduce the formation of deep wrinkles caused by collagen loss; how to develop eye care formulas with highly effective anti-wrinkle effects specifically for the characteristics of the skin around the eyes to combat dynamic and static wrinkles; how to comprehensively utilize antioxidants, moisturizers, and repair factors to consolidate the overall anti-wrinkle effect, strengthen the skin barrier function, resist free radical damage, and promote the skin's self-repair ability; and how to ensure that the various compositions work synergistically through scientific formulation and specific processes to combat wrinkle formation from different skincare dimensions, providing users with a complete skincare solution.

[0008] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows.

[0009] A synergistic, multi-dimensional anti-wrinkle skincare system consisting of four uniquely formulated compositions: Composition 1, Composition 2, Composition 3, and Composition 4. Each composition contains specific active ingredients and a specific manufacturing process, and uses yeast fermentation products as the base solvent at a concentration of 20-60%.

[0010] The composition 1 involved in this invention comprises the following components and addition ratios: Phase A (water-soluble matrix): deionized water: 10%-20%; Phase A1 (polyol moisturizing system): 1,3-propanediol: 0.5%-2%, glycerol: 1%-8%, butylene glycol: 0.1%-0.5%, 1,2-hexanediol: 0.2%-2%; Phase B (bioactive complex): yeast fermentation product: 25%-40%, cucumber fruit extract: 1%-5%, truffle extract: 1%-5%; and other active ingredients such as trehalose, Bifida ferment lysate, etc., are added in proportions within the specified range.

[0011] Phase A (water-soluble matrix)

[0012] Deionized water: As the main solvent carrier, it accounts for 20%-40% of the total weight of the composition. The purity of deionized water helps dissolve and stabilize other active ingredients, ensuring that the active ingredients can be better absorbed and utilized by the skin.

[0013] Phase A1 (Multifunctional Moisturizing Complex System)

[0014] 1,3-Propanediol: As a high-quality moisturizer and penetration enhancer, its content ranges from 0.5% to 2%. It not only has good moisturizing properties, but also enhances the penetration efficiency of other active substances in the skin.

[0015] Glycerin: As a naturally sourced moisturizer, its addition ratio of 1%-8% can significantly increase the moisture content of the stratum corneum and give the skin a lasting moisturizing feeling.

[0016] Butylene glycol: This ingredient accounts for 0.1%-0.5% of the product and has both moisturizing and skin feel regulating functions, while also helping to maintain product stability.

[0017] 1,2-Hexanediol: Added at a ratio of 0.2%-2%, in addition to its moisturizing effect, it can also improve product safety and extend shelf life due to its antibacterial and preservative properties.

[0018] Phase B (a blend of bioactive and plant extract components)

[0019] Yeast fermentation products: As the core bioactive ingredient, its content accounts for 25%-40% of the total weight of the composition. It is rich in a variety of amino acids, trace elements and bioactive factors, which can activate skin metabolism, enhance cell vitality and promote healthy skin.

[0020] Cucumber fruit extract: Added at a concentration of 1%-5%, with its excellent moisturizing and soothing properties, it helps to reduce skin fatigue and sensitivity, providing immediate and long-lasting hydration.

[0021] Black truffle extract and trehalose, among other plant extracts, are added at a ratio of 1% to 5%. These naturally derived nutrients work together to build the skin barrier function, deeply nourishing and repairing damaged skin.

[0022] Crepita yeast ferment lysate: Added at a ratio of 1%-5%, it exerts anti-aging effects by stimulating collagen synthesis, reducing wrinkle formation, and increasing skin elasticity.

[0023] Other carefully selected active ingredients include Tremella fuciformis extract, wild soybean seed extract, niacinamide, panthenol, hydroxypropyl tetrahydropyrantriol, vitamin B12, purslane extract, pine mushroom extract, Lactobacillus / pomegranate fruit ferment extract, yeast / grape ferment extract, Inonotus obliquus extract, saccharide isomers, Porphyra yezoensis extract, apple fruit extract, Lactobacillus ferment, ginseng root extract, peony root extract, wolfberry fruit extract, tea extract, and ascorbate tetraisopalmitate, with each ingredient's addition strictly controlled between 0.1% and 5%. These ingredients possess multiple effects such as anti-oxidation, anti-inflammation, whitening, firming, and moisturizing, working synergistically to provide a comprehensive and deep skincare experience.

[0024] The present invention relates to composition 2, with the following compositional components and addition ratio ranges: Phase A: Glycerin: 1%-2%, Inulin Lauryl Carbamate: 0.5%-1%, Squalane: 0.1%-1%; Phase B: Tremella fuciformis fermentation water: 20%-40%, 1,3-propanediol: 1%-10%, Glyceryl Polyether-26: 1%-5%, Sodium Hyaluronate: 0.03%-0.1%; Other active ingredients such as Ganoderma lucidum extract, etc.; Phase B1: 1,2-Hexanediol: 0.2%-1%, p-hydroxyacetophenone: 0.1%-0.5%, Xanthan Gum: 0.01%-0.1%; Phase C: A complex containing 35% hydroxypropyl tetrahydropyranotriol: 20%-30%, a complex containing 75% Bifida Ferment Lysate: 10%-20%; and also contains various peptide complexes such as... Argine500plus -COLL, etc.;

[0025] Detailed formula composition:

[0026] Phase A (Oily matrix)

[0027] Glycerin: As a basic moisturizing ingredient, it is added at a ratio of 1%-2%, which can effectively lock in skin moisture and provide long-lasting moisturizing effect.

[0028] Inulin lauryl carbamate: As a naturally sourced emulsifier and stabilizer, its addition of 0.5%-1% helps improve product stability and skin feel.

[0029] Squalane: 0.1%-1%, with excellent skin affinity and permeability, can replenish sebum and strengthen the skin barrier function.

[0030] Phase B (water-soluble nutrient solution)

[0031] Fermented white fungus water: accounting for 20%-40%, it is rich in various polysaccharides and has excellent moisturizing properties as well as the effect of promoting collagen production.

[0032] 1,3-Propanediol: The addition amount is 1%-10%, which acts as both a moisturizer and an aid in the dissolution of other active ingredients.

[0033] Glyceryl polyether-26: Added at a ratio of 1%-5%, it enhances the product's moisturizing power and improves the user experience.

[0034] Sodium hyaluronate: with a content of 0.03%, it enhances the product's moisturizing effect by utilizing its strong hygroscopic and water-retaining capabilities.

[0035] Reishi mushroom extract: formulated at a ratio of 1%-5%, it is rich in various bioactive substances and has antioxidant, anti-inflammatory and repairing effects.

[0036] Acrylic (ester) crosspolymer / C10-30 alkanol acrylate crosspolymer (thickener): Add 0.1%-0.5% to adjust product viscosity and texture.

[0037] Disodium EDTA (chelating agent): The dosage is 0.01%-0.05% to prevent the influence of metal ions on the active ingredients and maintain product stability.

[0038] Deionized water: accounting for 5%-15%, serving as a carrier and dispersion medium to ensure uniform mixing of all components.

[0039] Phase B1 (Stabilizer and Corrosion Inhibitor System)

[0040] 1,2-Hexanediol: The addition amount is controlled at 0.2%-1%, which has both moisturizing and antibacterial functions.

[0041] p-Hydroxyacetophenone: As a mild and effective preservative, its addition range is 0.1%-0.5%.

[0042] Xanthan gum: with a content of 0.01%-0.1%, it is used as a thickener and suspending agent to ensure product texture stability.

[0043] Phase C (highly active complex ingredient)

[0044] The complex ingredient contains 35% hydroxypropyl tetrahydropyrantriol: added at a ratio of 10%-30%, it significantly increases the skin's moisture content and reduces fine lines.

[0045] The complex contains 75% Bifida ferment lysate, accounting for 10%-20% of the total composition. It is rich in amino acids and trace elements, which can activate cell metabolism and delay the aging process.

[0046] Butylene glycol: Added at a rate of 1%-10%, it further enhances moisturizing efficacy.

[0047] The present invention relates to composition 3: Composition and addition ratio range: Phase A: Yeast fermentation product: 20-40%; Deionized water: 10-30%; Phase A1: Disodium EDTA: 0.01-0.05%; Allantoin: 0.1-0.3%; 1,3-propylene glycol: 1-3%; Dipropylene glycol: 1-3%; 1,2-hexanediol: 1-3%; Sodium hyaluronate: 0.01-0.05%; Phase B: MATRIXYL 3000MBAL composition containing palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7: 8-10%; Polydimethylsiloxane: 10-25%; Composite component containing 35% hydroxypropyl tetrahydropyranotriol: 5-10%; Sodium acrylate / sodium acryloyldimethyl taurate copolymer: 2-5%; Bifida ferment lysate: 2-5%; Composition containing acetyl hexapeptide-8. Argine500plus: 2%-5%; Compositions containing palmitoyl tripeptide-5 - COLL: 1-2%; Composition containing arginine / lysine peptides: 1-5%; Aloe vera leaf juice: 1-5%; *Porphyra yezoensis* extract: 1-5%; Hydroxydecyl ubiquinone: 0.1-0.5%; *Stephania tetrandra* extract: 1-5%; *Echinacea purpurea* extract: 1-5%; Arginine / lysine peptide powder: 0.1-0.5%; Apple fruit extract: 1-5%; Hydrolyzed collagen: 0.1-0.5%; Caffeine: 0.1-0.5%.

[0048] Detailed formula composition:

[0049] Phase A (core fermentation product substrate)

[0050] Yeast fermentation products: As the core source of active ingredients, they account for 20%-40% of the total content and are rich in a variety of bioactive substances, which can effectively promote cell metabolism and collagen synthesis.

[0051] Deionized water: As a solvent and diluent, it is added at a rate of 10%-30% to ensure that fermentation products and other water-soluble components are fully dissolved and evenly dispersed.

[0052] Phase A1 (stabilizing and moisturizing additive)

[0053] Disodium EDTA: As a chelating agent, it prevents metal ions from affecting product stability. The addition ratio is 0.01%-0.05%.

[0054] Allantoin: It has good moisturizing, soothing and anti-inflammatory effects, and the addition amount is between 0.1% and 0.3%.

[0055] 1,3-Propylene glycol, dipropylene glycol, and 1,2-hexanediol: These three polyol moisturizers are added at a ratio of 1% to 3% to enhance the product's moisturizing properties and skin feel.

[0056] Sodium hyaluronate: accounting for 0.01%-0.05%, it is widely used in skin care products due to its excellent water retention and penetration capabilities, further enhancing the moisturizing effect of the products.

[0057] Phase B (Multifunctional Active Complex System)

[0058] MATRIXYL 3000MBAL, a composition containing palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, is added at a ratio of 8-10% to intervene in signs of skin aging, stimulate collagen production, and improve wrinkles.

[0059] Polydimethylsiloxane: As a softener and film-forming agent, it is added at a rate of 10%-25%, giving the product a smooth feel and long-lasting moisturizing effect.

[0060] The complex contains 35% hydroxypropyl tetrahydropyrantriol: contained in a 5-10% ratio, it helps increase skin elasticity and reduce fine lines.

[0061] Sodium acrylate / sodium acryloyl dimethyl taurate copolymer: As a thickener and stabilizer, it is added at a ratio of 2-5% to ensure the product has a stable texture and is easy to apply.

[0062] Bifida ferment lysate: also accounting for 2-5%, it contains rich amino acids, peptides and trace elements, which help accelerate skin renewal and enhance protective ability.

[0063] Compositions containing acetyl hexapeptide-8 Argine500plus: The dosage is 2%-5%, which can inhibit neuromuscular contraction and reduce expression lines.

[0064] Compositions containing palmitoyl tripeptide-5 -COLL: Add at a rate of 1-2% to target skin aging problems and strengthen the collagen network structure.

[0065] Compositions containing arginine / lysine peptides: possess antioxidant and repair functions, with an addition range of 1-5%.

[0066] Aloe vera leaf juice, purple algae extract, purple coneflower extract, and apple fruit extract: These plant extracts are added at a ratio of 1-5% each, together providing multiple benefits such as anti-oxidation, soothing, moisturizing, and nourishing.

[0067] Hydroxydecyl ubiquinone (Coenzyme Q10): As a powerful antioxidant, the addition amount is 0.1-0.5%, which fights free radical damage and delays the skin aging process.

[0068] Stephania tetrandra extract: It has certain anti-inflammatory and antioxidant effects, and the addition ratio is 1-5%.

[0069] Arginine / Lysine polypeptide powder: also has repair and protective effects, and the concentration used is 0.1-0.5%.

[0070] Hydrolyzed collagen: with a content of 0.1-0.5%, it replenishes the collagen lost by the skin with its excellent skin affinity and permeability.

[0071] Caffeine: As a vasoconstrictor, it can temporarily reduce eye bags and puffiness. The amount added should be controlled at 0.1-0.5%.

[0072] The composition 4 involved in this invention comprises the following components and addition ratios: Phase A: C12-20 PEG-8 ester: 6%; Glycerin 0.5-1%; Tris(octyldodecanoyl) citrate: 3-6%; Tocopherol (Vitamin E): 1-5%; Caprylic / Capric triglyceride: 3-8%; Phenyl polytrimethylsiloxane: 1-3%; Sunflower seed oil: 0.1-0.5%; Cetearyl alcohol: 1-3%; Polydimethylsiloxane: 1-5%; Tocopheryl acetate: 0.1-1%;

[0073] Phase B: Deionized water: 15-30%; Isopentyl glycol: 5-8%; Potassium cetyl phosphate: 0.1-0.3%; Fermented water from tremella: 20-45%; Ammonium acryloyl dimethyl taurate / VP copolymer: 0.5-1%; Sodium hyaluronate: 0.05-0.1%; 1,2-hexanediol: 0.2-1%; p-hydroxyacetophenone: 0.2-0.4%; Black goji berry puree: 2-5%;

[0074] Phase C: 1%-2% of a complex containing 35% hydroxypropyl tetrahydropyrantriol; 0.5-1% of a composition containing arginine / lysine peptides; 1-5% of a complex fermented water containing grape, pomegranate, and birch polypore; 1-5% of barley and tremella fuciformis puree; 1-5% of purslane puree; 1-5% of ergothioneine and pine mushroom extract; 1-5% of *Porphyra yezoensis* extract; 0.1-1% of bisabolol; 0.15-1% of tetrahydromethylpyrimidine carboxylic acid; 0.15-1% of Purple Duo (double purple oil); 0.1-1% of a plant-based soothing agent; 0.15-1% of a combination containing alanine, glutamic acid, aspartic acid, nicotinic acid hexyl ester, and glucose; and a combination of saccharide isomers. 1-5%; Cucumber fresh water: 1-5%; Caffeine: 0.2-1%;

[0075] Detailed formula composition:

[0076] Phase A (oil and lipid components)

[0077] C12-20 acid PEG-8 ester: used as an emulsifier and emollient, at a content of 6%.

[0078] Glycerin: As a powerful moisturizer, it is added at a rate of 0.5%-1% to enhance the product's water retention capacity.

[0079] Tris(octyldodecyl)citrate: used as a softener and thickener, accounting for 3%-6%.

[0080] Tocopherol (Vitamin E): It has antioxidant properties and protects the skin from free radical damage. The addition ratio is 1%-5%.

[0081] Caprylic / capric triglycerides: As an excellent moisturizing ingredient, the dosage is between 3% and 8%.

[0082] Phenyl polytrimethylsiloxane: imparts a silky feel to the product; the addition amount is 1%-3%.

[0083] Sunflower seed oil: Rich in vitamin E and essential fatty acids, it nourishes the skin, and its addition range is 0.1%-0.5%.

[0084] Cetearyl alcohol: As an emulsifying stabilizer, it also has certain moisturizing properties, and the addition amount is 1%-3%.

[0085] Polydimethylsiloxane: Improves skin feel and increases product smoothness; the addition ratio is 1%-5%.

[0086] Tocopheryl acetate: Further enhances antioxidant effects, with a content of 0.1%-1%.

[0087] Phase B (water-soluble active ingredients and auxiliaries)

[0088] Deionized water: used as the main solvent and carrier, accounting for 15%-30%.

[0089] Isoamyl glycol: It has moisturizing and stabilizing effects, and the addition amount is 5%-8%.

[0090] Potassium cetyl phosphate: As a mild surfactant and emulsifier, it is added at a rate of 0.1%-0.3%.

[0091] Fermented white fungus water: rich in polysaccharides and amino acids, with moisturizing and repairing effects, and a content of 20%-45%.

[0092] Acryloyldimethyl taurate ammonium / VP copolymer: used as a thickener and stabilizer, added at a rate of 0.5%-1%.

[0093] Sodium hyaluronate: an excellent moisturizing factor that increases skin hydration, with a content of 0.05%-0.1%.

[0094] 1,2-Hexanediol: Used as a humectant and antibacterial preservative, added at a rate of 0.2%-1%.

[0095] p-Hydroxyacetophenone: a natural antioxidant and preservative, added at a rate of 0.2%-0.4%.

[0096] Black goji berry puree: rich in various nutrients, with anti-aging and antioxidant effects, and the recommended concentration is 2%-5%.

[0097] Phase C (Multi-Active Complex Essence)

[0098] Contains a complex ingredient with 35% hydroxypropyl tetrahydropyrantriol: effectively improves skin elasticity and reduces fine lines, with an addition amount of 1%-2%.

[0099] Compositions containing arginine / lysine peptides: promote skin cell renewal and enhance the skin barrier; the addition ratio is 0.5%-1%.

[0100] Grape, pomegranate, birch polypore compound fermented water: contains rich bioactive substances, with multiple functions such as moisturizing and repairing, and the content is 1%-5%.

[0101] Barley and Tremella Extract, Purslane Extract: Natural plant extracts that provide soothing, moisturizing, and antioxidant effects, each added at a ratio of 1%-5%.

[0102] Ergothioneine Pine Mushroom Extract: It has significant antioxidant and anti-inflammatory activity, and the addition amount is 1%-5%.

[0103] Purple algae extract: rich in various trace elements and polysaccharides, it has a good moisturizing and firming effect on the skin, and the addition range is 1%-5%.

[0104] Bisabolol: As an anti-inflammatory, antibacterial, and soothing ingredient, it is added at a rate of 0.1%-1%.

[0105] Tetrahydromethylpyrimidine carboxylic acid: Helps maintain skin's moisture balance and radiance; added at a rate of 0.15%-1%.

[0106] Purple Duo: A precious plant oil complex that provides deep nourishment and repair, added at a rate of 0.15%-1%.

[0107] Plant-based soothing agents: They have good anti-allergic and sedative effects, and the addition amount is 0.1%-1%.

[0108] It contains a combination of ingredients including alanine, glutamic acid, aspartic acid, nicotinic acid hexyl ester, and glucose: which synergistically provide multiple effects such as moisturizing and repair, with an addition ratio of 0.15%-1%.

[0109] Composition of carbohydrate isomers Highly effective moisturizing factors enhance skin's hydration ability; added at a concentration of 1%-5%.

[0110] Cucumber Fresh Water: Rich in moisture and nutrients, providing a cool and comfortable feel. Add at a ratio of 1%-5%.

[0111] Caffeine: Helps improve microcirculation and reduce eye puffiness; the recommended dosage is 0.2%-1%.

[0112] This invention proposes a method for preparing a synergistic, multi-dimensional anti-wrinkle skincare product system.

[0113] Composition 1 is prepared by heating and mixing phase A and phase A1 at 85°C, cooling to 45°C, and then adding phase B active ingredients. It is used to improve the moisture content of the stratum corneum and overall skin health. The detailed preparation method of Composition 1 is as follows.

[0114] Preparation of phase A (water-soluble matrix):

[0115] Take a certain amount of deionized water and add it to the main reaction vessel at a ratio of 10%-20% as the main solvent and carrier of the product.

[0116] Preparation of phase A1 (mixture of polyols and moisturizing additives):

[0117] In a separate container, 1,3-propanediol is added sequentially at a concentration between 0.5% and 2% to enhance the product's moisturizing properties and stability.

[0118] Next, add glycerin (1%-8%) in proportion to further enhance the product's moisturizing effect.

[0119] Then, butylene glycol (0.1%-0.5%) and 1,2-hexanediol (0.2%-2%) are added respectively. These two ingredients can effectively improve the product's moisturizing properties and skin feel.

[0120] Preparation of Phase B (bioactive complex and plant extract):

[0121] In a separate container, first mix the yeast fermentation product at a ratio of 25%-40% as the main source of biological activity.

[0122] Next, cucumber fruit extract (1%-5%), black truffle extract (1%-5%), and other plant extracts such as trehalose (1%-5%) are added one by one.

[0123] Add Bifida ferment lysate, which has repairing and moisturizing effects, at a content of 0.5%-5%.

[0124] It also needs to add nutrients such as tremella extract (0.2%-1%) and wild soybean seed extract (0.2%-1%), as well as a variety of skin-beneficial active substances such as niacinamide (0.2%-1%) and panthenol (0.2%-1%).

[0125] Finally, according to the formula requirements, add appropriate amounts of hydroxypropyl tetrahydropyrantriol (0.5%-5%), vitamin B12 (0.1%-2%), purslane extract (0.1%-2%), pine mushroom extract (0.1%-5%), and a series of other plant fermentation extracts and antioxidants to ensure that each component is fully dissolved and evenly dispersed.

[0126] Final formulation steps:

[0127] First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0128] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0129] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0130] Composition 2 involves a stepwise heating process to fuse phases A, B, and B1, followed by the addition of peptide complexes and other active substances from phase C at 45°C. This aims to promote cell renewal and enhance dermal elasticity. The specific preparation process for Composition 2 is as follows.

[0131] Preparation of phase A (water-oil equilibrium phase):

[0132] First, add glycerin (1%-2%) to the main reaction vessel in the specified proportion as a moisturizing and lubricating ingredient.

[0133] Next, inulin lauryl carbamate (0.5%-1%) is added to enhance the product's stability and emulsification.

[0134] Then, squalane (0.1%-1%) is added as an excellent skin softener and emollient.

[0135] Preparation of Phase B (water-soluble nutrient solution):

[0136] In another container, first add 20%-40% of the fermented white fungus water as a base carrier.

[0137] Adding 1,3-propanediol (1%-10%) enhances the product's moisturizing properties and helps dissolve and disperse active ingredients.

[0138] Add glyceryl polyether-26 (1%-5%) to provide additional moisturizing and penetrating effects.

[0139] Next, add sodium hyaluronate (0.03%-0.1%) to improve the skin's ability to retain moisture.

[0140] Reishi mushroom extract (1%-5%) is also added to provide the product with antioxidant and repairing effects.

[0141] In addition, an appropriate amount of acrylate / C10-30 alkanol acrylate crosspolymer (0.1-0.5%) was added as a thickener.

[0142] Disodium EDTA (0.01%-0.05%) acts as a chelating agent, which can chelate metal ions to protect product stability.

[0143] Finally, add deionized water (5-15%) to ensure the homogeneity and stability of the entire aqueous system.

[0144] Preparation of B1 phase (auxiliary stabilizing corrosion protection system):

[0145] 1,2-Hexanediol (0.2%-1%), p-hydroxyacetophenone (0.1%-0.5%), and xanthan gum (0.01%-0.1%) were mixed separately in individual containers to form an effective stabilizing and preservative system.

[0146] Preparation of phase C (highly effective anti-wrinkle complex phase):

[0147] A compound containing 35% hydroxypropyl tetrahydropyrantriol (20%-30%) and a compound containing 75% Bifida ferment lysate (10%-20%) are prepared according to a specific ratio.

[0148] It also includes solvents such as butylene glycol (1-10%) and deionized water (5-15%), as well as... Argine500 plus (5%-8%) is a compound ingredient containing acetyl hexapeptide-8.

[0149] At the same time, join -COLL (a complex of palmitoyl tripeptide-5, 3-5%) and carnosine powder (0.1-0.5%) and other peptide combinations.

[0150] In addition, it also includes a variety of plant extracts such as ginseng extract, Ganoderma lucidum extract, cactus extract, and Echinacea purpurea extract, with each extract added in an amount ranging from 1% to 5%.

[0151] Supplement with other functional ingredients such as vitamin B12 (2-5%), polyglycerol-10 myristate (0.5-2%), and arginine (0.1-1%).

[0152] Final formulation steps:

[0153] First, heat phase A to 85 degrees Celsius and stir until completely dissolved.

[0154] Phase B and Phase B1 were pretreated separately, and then heated to 85 degrees Celsius and thoroughly stirred and dispersed.

[0155] After phase A has cooled to below 45 degrees Celsius, add the pre-prepared phase B and phase B1 in sequence, and continue stirring until all components are uniformly dispersed in the system.

[0156] Finally, the pre-prepared C-phase component is gradually added at a suitable temperature, and the components are evenly dispersed in the product by gentle stirring, thus obtaining the final product, composition 2.

[0157] Composition 3 is specifically designed for eye wrinkle care. After dissolving and dispersing phases A and A1 at high temperature, phase B active ingredients are added at low temperature. It contains a specific ratio of peptides and moisturizers to combat both dynamic and static wrinkles around the eyes. The detailed preparation method of composition 3 is as follows.

[0158] Preparation of Phase A (mixture of yeast fermentation product and aqueous phase):

[0159] First, yeast fermentation products are added to the main reaction vessel at a ratio of 20%-40%, serving as the main active ingredient. Then, deionized water is added to the same vessel at a ratio of 10%-30%, and thoroughly mixed with the yeast fermentation products.

[0160] Preparation of A1 phase (stabilizing and moisturizing additive):

[0161] In a separate container, add the ingredients in the following proportions and stir well:

[0162] Disodium EDTA: 0.01%-0.05%, allantoin: 0.1%-0.3%, 1,3-propylene glycol: 1-3%, dipropylene glycol: 1-3%, 1,2-hexanediol: 1-3%, sodium hyaluronate: 0.01%-0.05%.

[0163] Preparation of Phase B (polypeptide and plant extract complex system):

[0164] In another container, prepare a mixture containing the following active ingredients:

[0165] MATRIXYL 3000MBAL, a composition of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, is added at a rate of 8%-10%. Polydimethylsiloxane is added at a rate of 10%-25%. A complex containing 35% hydroxypropyl tetrahydropyranotriol is added at a rate of 5%-10%.

[0166] Other thickeners and stabilizers include sodium acrylate / sodium acryloyl dimethyl taurate copolymer and various peptide complexes (including compound components containing acetyl hexapeptide-8). Argine500plus contains Argine500Plus, as well as various plant extracts (such as aloe vera leaf juice, purple algae extract, etc.), with the addition ratio of each extract controlled within the range of 1%-5%.

[0167] Final formulation steps:

[0168] First, heat phase A to 85 degrees Celsius to ensure that the yeast fermentation products are fully mixed with deionized water.

[0169] Each component in phase A1 is heated to the same temperature to completely dissolve and uniformly disperse it.

[0170] When phase A is cooled to below 45 degrees Celsius, the pre-prepared phase A1 is gradually added and stirred continuously to ensure that the two are fully mixed.

[0171] Then, the prepared phase B is added to the above mixture one by one, and the mixture is stirred at a constant temperature to ensure that all active ingredients are evenly dispersed in the entire product system to form a stable skin care composition 3.

[0172] Composition 4 utilizes oil-water emulsification technology to combine phase A (oil components) with phase B (water-soluble components) at a specific temperature, then adds phase C (repairing and moisturizing components), and stirs evenly below 45°C to form a holistic skincare formula with enhanced anti-wrinkle effects. The detailed preparation method of Composition 4 is as follows.

[0173] Preparation of phase A (oily substrate):

[0174] In the main reaction vessel, C12-20 acid PEG-8 ester is first added as the main emulsifier and emollient, with an addition ratio of 3%-6%.

[0175] Next, glycerol (0.5%-1%), tri(octyldodecanoyl) citrate (3%-6%), tocopherol (vitamin E) (1%-5%), caprylic / capric triglyceride (3%-8%), phenyl polytrimethylsiloxane (1%-3%), sunflower seed oil (0.1%-0.5%), cetearyl alcohol (1%-3%), and polydimethylsiloxane (1%-5%) are added according to the formula requirements to form a stable oil phase base.

[0176] Finally, tocopheryl acetate (0.1%-1%) is added to the oil phase to enhance the antioxidant properties of the product.

[0177] Preparation of Phase B (water-soluble nutrient solution):

[0178] In a separate container, add deionized water (15%-30%), fermented tremella water (20%-45%), isopentyl glycol (5%-8%), and an appropriate amount of thickening and stabilizing agent such as potassium cetyl phosphate (0.1%-0.3%) in the following proportions.

[0179] It contains moisturizing and skin-conditioning ingredients, including ammonium acryloyl dimethyl taurate / VP copolymer (0.5%-1%) and sodium hyaluronate (0.05%-0.1%).

[0180] It should also contain certain antioxidant and soothing ingredients, such as 1,2-hexanediol (0.2%-1%), p-hydroxyacetophenone (0.2%-0.4%), and black goji berry puree (2%-5%).

[0181] Preparation of phase C (highly active complex mixture):

[0182] The composition of a compound containing 35% hydroxypropyl tetrahydropyrantriol (1%-2%) and an arginine / lysine peptide (0.5%-1%) is prepared separately.

[0183] Prepare a complex fermented water of grape, pomegranate, and birch polypore (1%-5%), barley and tremella fuciformis extract (1%-5%), and other plant extracts, each added in amounts between 1% and 5%, to ensure the final product has comprehensive anti-wrinkle and repairing effects.

[0184] It also contains specific active ingredients such as bisabolol (0.1%-1%), tetrahydromethylpyrimidine carboxylic acid (0.15%-1%), and Purple Duo (0.15%-1%).

[0185] To enhance overall hydration and soothe the skin, it also includes plant-based soothing agents (0.1%-1%) and ingredients containing a specific combination of amino acids (alanine, glutamic acid, aspartic acid, nicotinic acid hexyl ester, glucose) (0.15%-1%).

[0186] Finally, supplement the carbohydrate isomer combination components. (1%-5%), as well as effective active ingredients such as cucumber fresh water (1%-5%) and caffeine (0.2%-1%).

[0187] Final formulation step:

[0188] The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0189] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0190] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0191] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0192] This invention proposes a method for using a synergistic, multi-dimensional anti-wrinkle skincare system, which consists of four uniquely formulated compositions: Composition 1, Composition 2, Composition 3, and Composition 4. The specific method of use is as follows:

[0193] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed;

[0194] Composition 1 Application: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, gently massaging until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin;

[0195] Application of Composition 2: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application of Composition 2 can be skipped, and you can proceed directly to the next step.

[0196] Composition 3 Targeted Treatment: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0197] Composition 4 for full coverage: After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all previous steps in the final step, providing continuous nourishment and protection;

[0198] Following the above usage procedure, perform this skincare routine once in the morning and once in the evening. Depending on actual needs, users can adjust the skincare steps during the day and use them flexibly to achieve the best skincare results.

[0199] Due to the adoption of the above technical solutions, the technical progress achieved by this invention is as follows.

[0200] In the skincare system of this invention, the four compositions have a synergistic effect. When used in a specific order, they can maximize the improvement of various wrinkles caused by skin aging, comprehensively addressing skin aging from multiple dimensions. This represents a significant technological advancement in the field of skin anti-aging, particularly in wrinkle prevention and treatment, mainly reflected in the following aspects:

[0201] Multi-dimensional synergistic effect: Unlike traditional anti-wrinkle products with single or limited mechanisms, this invention achieves comprehensive intervention against multiple key factors in the skin aging process through four carefully designed and formulated compositions. Each composition is optimized for different causes of aging, such as moisturizing, anti-oxidation, anti-glycation, and cell renewal, ensuring a comprehensive and three-dimensional anti-wrinkle effect.

[0202] Deeply nourishing and anti-wrinkle: The composition contains highly effective moisturizing ingredients (such as glycerin, hyaluronic acid, etc.), bioactive peptides (such as pentapeptide, hexapeptide, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, etc.) and a variety of plant extracts. These ingredients can penetrate deep into all layers of the skin to provide nutrition, stimulate collagen synthesis, enhance the elasticity of the dermis, and improve and prevent wrinkles caused by moisture loss and collagen reduction.

[0203] Customized eye care: The specially developed formula for the eyes effectively combats the static and dynamic wrinkles unique to the eye area. It uses a unique combination of signal peptides and highly effective anti-wrinkle agents to enhance targeted care for the specific characteristics of the skin around the eyes.

[0204] Scientific and rational formulation and process: Through precise control of various active ingredients and specific processing techniques, the synergistic effect between components is maximized, avoiding mutual cancellation between ingredients and enhancing the overall skincare efficacy. For example, products containing Pro-Xylane not only promote fibroblast proliferation and basement membrane repair, but also combine with other compound ingredients to achieve simultaneous anti-aging of the epidermis and dermis.

[0205] Comprehensive coverage of skin aging: By integrating various active substances such as antioxidants, repair factors, and moisturizers, it not only focuses on the healthy maintenance of the stratum corneum, but also takes into account the activation of the regenerative capacity of the dermis, the scavenging of free radicals, the inhibition of glycation reactions, and the optimization of cell renewal rate, thereby comprehensively improving the overall health and anti-aging ability of the skin.

[0206] In summary, this invention, with its systematic, comprehensive, and unique approach, has achieved an innovative breakthrough in the field of anti-aging skincare technology. It provides users with a more efficient, safe, and long-lasting anti-wrinkle solution, filling the gap in the existing market for multi-dimensional synergistic effects and meeting consumers' needs for personalized and refined skincare solutions. Attached Figure Description

[0207] Figure 1 This is a comparison chart showing the improvement of facial wrinkles before and after four weeks of using the anti-wrinkle skincare system of Example 1 of the present invention. Detailed Implementation

[0208] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0209] Example 1:

[0210] The components and proportions of composition 1 in Example 1 are as follows:

[0211]

[0212]

[0213] Preparation method of composition 1 in Example 1: First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0214] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0215] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0216] The composition and proportions of composition 2 in Example 1:

[0217]

[0218]

[0219] Preparation method of composition 2 in Example 1: First, heat phase A to 85 degrees Celsius and stir until completely dissolved. Pre-treat phases B and B1 separately, heating them to 85 degrees Celsius and stirring thoroughly to disperse them evenly. After phase A cools to below 45 degrees Celsius, add the pre-prepared phases B and B1 sequentially, continuing to stir until all components are uniformly dispersed in the system. Finally, gradually add the pre-prepared phase C component at a suitable temperature, gently stirring to ensure uniform dispersion of all components in the product, thus obtaining the final composition 2.

[0220] The composition and proportions of composition 3 in Example 1 are as follows:

[0221]

[0222]

[0223] Preparation method of composition 3 in Example 1: First, heat phase A to 85 degrees Celsius to ensure that the yeast fermentation product and deionized water are fully mixed. Heat each component in phase A1 to the same temperature to completely dissolve and uniformly disperse them. When phase A cools to below 45 degrees Celsius, gradually add the pre-prepared phase A1 while continuously stirring to ensure thorough mixing. Then, add the prepared phase B to the above mixture sequentially, maintaining a constant temperature and stirring to ensure that all active ingredients are uniformly dispersed throughout the product system, forming a stable skincare composition 3.

[0224] The composition and proportions of composition 4 in Example 1 are as follows:

[0225]

[0226]

[0227]

[0228] Preparation method of composition 4 in Example 1: The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0229] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0230] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0231] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0232] The specific usage method of Example 1 is as follows:

[0233] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed.

[0234] Application of Composition 1 in Example 1: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, and gently massage until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin.

[0235] In Example 1, applying Composition 2: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application step of Composition 2 can be omitted, and proceed directly to the next step.

[0236] In Example 1, Composition 3 is used for targeted treatment: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0237] In Example 1, Composition 4 provides full coverage: After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all previous steps in the final step, providing continuous nourishment and protection.

[0238] Following the above usage procedure, perform this skincare routine once in the morning and once in the evening. Depending on actual needs, users can adjust the skincare steps during the day and use them flexibly to achieve the best skincare results.

[0239] The improvement in facial wrinkles after four weeks of using the anti-wrinkle skincare system of Example 1 is compared to... Figure 1 As shown in the figure, the fine lines are significantly reduced after four weeks of use in this embodiment.

[0240] Example 2:

[0241] The components and proportions of composition 1 in Example 2 are as follows:

[0242]

[0243]

[0244] Preparation method of composition 1 in Example 2: First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0245] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0246] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0247] The components and proportions of composition 2 in Example 2 are as follows:

[0248]

[0249]

[0250] The preparation method of composition 2 in Example 2 is as follows: Phase A is first heated to 85 degrees Celsius and stirred until completely dissolved. Phases B and B1 are pretreated separately, also heated to 85 degrees Celsius and stirred thoroughly to disperse evenly. After phase A cools to below 45 degrees Celsius, the pre-prepared phases B and B1 are added sequentially, and stirring is continued until all components are uniformly dispersed in the system. Finally, the pre-prepared phase C component is gradually added at a suitable temperature, and the components are uniformly dispersed in the product by gentle stirring, thereby obtaining the final product, composition 2.

[0251] The composition and proportions of composition 3 in Example 2 are as follows:

[0252]

[0253]

[0254] The preparation method of composition 3 in Example 2 is as follows: First, heat phase A to 85 degrees Celsius to ensure that the yeast fermentation product and deionized water are fully mixed. Heat each component in phase A1 to the same temperature to completely dissolve and uniformly disperse them. When phase A cools to below 45 degrees Celsius, gradually add the pre-prepared phase A1 while continuously stirring to ensure thorough mixing. Then, add the prepared phase B to the above mixture sequentially, maintaining a constant temperature and stirring to ensure that all active ingredients are uniformly dispersed throughout the product system, forming a stable skincare composition 3.

[0255] The composition and proportions of composition 4 in Example 2 are as follows:

[0256]

[0257]

[0258] Preparation method of composition 4 in Example 2: The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0259] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0260] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0261] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0262] The specific usage method of Example 2 is as follows:

[0263] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed.

[0264] Application of Composition 1 in Example 2: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, and gently massage until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin.

[0265] In Example 2, applying Composition 2: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application step of Composition 2 can be omitted, and proceed directly to the next step.

[0266] In Example 2, Composition 3 is used for targeted treatment: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0267] In Example 2, Composition 4 provides full coverage: After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all preceding steps in the final step, providing continuous nourishment and protection.

[0268] Following the above usage procedure, perform this skincare routine once in the morning and once in the evening. Depending on actual needs, users can adjust the skincare steps during the day and use them flexibly to achieve the best skincare results.

[0269] Example 3:

[0270] The composition and proportions of composition 1 in Example 3 are as follows:

[0271]

[0272]

[0273] Preparation method of composition 1 in Example 3: First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0274] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0275] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0276] The composition and proportions of composition 2 in Example 3 are as follows:

[0277]

[0278]

[0279] Preparation method of composition 2 in Example 3: First, heat phase A to 85 degrees Celsius and stir until completely dissolved. Pre-treat phases B and B1 separately, heating them to 85 degrees Celsius and stirring thoroughly to disperse them evenly. After phase A cools to below 45 degrees Celsius, add the pre-prepared phases B and B1 sequentially, continuing to stir until all components are uniformly dispersed in the system. Finally, gradually add the pre-prepared phase C component at a suitable temperature, gently stirring to ensure uniform dispersion of all components in the product, thus obtaining the final composition 2.

[0280] The components and proportions of composition 3 in Example 3 are as follows:

[0281]

[0282]

[0283] The preparation method of composition 3 in Example 3 is as follows: First, heat phase A to 85 degrees Celsius to ensure that the yeast fermentation product and deionized water are fully mixed. Heat each component in phase A1 to the same temperature to completely dissolve and uniformly disperse them. When phase A cools to below 45 degrees Celsius, gradually add the pre-prepared phase A1 while continuously stirring to ensure thorough mixing. Then, add the prepared phase B to the above mixture sequentially, maintaining a constant temperature and stirring to ensure that all active ingredients are uniformly dispersed throughout the product system, forming a stable skincare composition 3.

[0284] The composition and proportions of composition 4 in Example 3 are as follows:

[0285]

[0286]

[0287] Preparation method of composition 4 in Example 3: The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0288] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0289] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0290] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0291] The specific usage method of Example 3 is as follows:

[0292] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed.

[0293] Application of Composition 1 in Example 3: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, and gently massage until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin.

[0294] In Example 3, applying Composition 2: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application step of Composition 2 can be omitted, and proceed directly to the next step.

[0295] In Example 3, Composition 3 is used for targeted treatment: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0296] In Example 3, Composition 4 provides full coverage: After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all previous steps in the final step, providing continuous nourishment and protection.

[0297] Following the above usage procedure, perform this skincare routine once in the morning and once in the evening. Depending on actual needs, users can adjust the skincare steps during the day and use them flexibly to achieve the best skincare results.

[0298] Example 4:

[0299] The composition and proportions of composition 1 in Example 4 are as follows:

[0300]

[0301]

[0302] Preparation method of composition 1 in Example 4: First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0303] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0304] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0305] The composition and proportions of composition 2 in Example 4 are as follows:

[0306]

[0307]

[0308] The preparation method of composition 2 in Example 4 is as follows: Phase A is first heated to 85 degrees Celsius and stirred until completely dissolved. Phases B and B1 are pretreated separately, also heated to 85 degrees Celsius and stirred thoroughly to disperse evenly. After phase A cools to below 45 degrees Celsius, the pre-prepared phases B and B1 are added sequentially, and stirring is continued until all components are uniformly dispersed in the system. Finally, the pre-prepared phase C component is gradually added at a suitable temperature, and the components are uniformly dispersed in the product by gentle stirring, thereby obtaining the final product, composition 2.

[0309] The composition and proportions of composition 3 in Example 4 are as follows:

[0310]

[0311]

[0312] Preparation method of composition 3 in Example 4: First, heat phase A to 85 degrees Celsius to ensure that the yeast fermentation product and deionized water are fully mixed. Heat each component in phase A1 to the same temperature to completely dissolve and uniformly disperse them. When phase A cools to below 45 degrees Celsius, gradually add the pre-prepared phase A1 while continuously stirring to ensure thorough mixing. Then, add the prepared phase B to the above mixture sequentially, maintaining a constant temperature and stirring to ensure that all active ingredients are uniformly dispersed throughout the product system, forming a stable skincare composition 3.

[0313] The components and proportions of composition 4 in Example 4 are as follows:

[0314]

[0315]

[0316] Preparation method of composition 4 in Example 4: The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0317] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0318] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0319] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0320] The specific usage method of Example 4 is as follows:

[0321] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed.

[0322] Application of Composition 1 in Example 4: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, and gently massage until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin.

[0323] In Example 4, applying Composition 2: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application step of Composition 2 can be omitted, and proceed directly to the next step.

[0324] In Example 4, Composition 3 is used for targeted treatment: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0325] In Example 4, Composition 4 provides full coverage: After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all preceding steps in the final step, providing continuous nourishment and protection.

[0326] Following the above usage procedure, perform this skincare routine once in the morning and once in the evening. Depending on actual needs, users can adjust the skincare steps during the day and use them flexibly to achieve the best skincare results.

[0327] Compare with Example 1:

[0328] The composition and proportions of composition 1 in Comparative Example 1:

[0329]

[0330]

[0331] Preparation method of composition 1 in Comparative Example 1: First, heat phase A and phase A1 to 85 degrees Celsius respectively to ensure that all water-soluble components are completely dissolved and uniformly mixed.

[0332] When the two-phase solution is cooled to below 45 degrees Celsius, the pre-prepared phase B components are slowly added one by one while continuously stirring to ensure that the various active ingredients can be stably dispersed in the entire system at low temperature.

[0333] After careful stirring and mixing until all components are evenly distributed, the final product, composition 1, is obtained.

[0334] The composition and proportions of composition 2 in Comparative Example 1:

[0335]

[0336]

[0337] The preparation method of composition 2 in Comparative Example 1 is as follows: Phase A is first heated to 85 degrees Celsius and stirred until completely dissolved. Phases B and B1 are pretreated separately, also heated to 85 degrees Celsius and stirred thoroughly to disperse evenly. After phase A cools to below 45 degrees Celsius, the pre-prepared phases B and B1 are added sequentially, and stirring is continued until all components are uniformly dispersed in the system. Finally, the pre-prepared phase C component is gradually added at a suitable temperature, and the components are uniformly dispersed in the product by gentle stirring, thereby obtaining the final product, composition 2.

[0338] The composition and proportions of composition 3 in Comparative Example 1:

[0339]

[0340]

[0341] The preparation method of composition 3 in Comparative Example 1 is as follows: First, phase A is heated to 85 degrees Celsius to ensure that the yeast fermentation product and deionized water are fully mixed. All components in phase A1 are heated to the same temperature to completely dissolve and uniformly disperse them. When phase A cools to below 45 degrees Celsius, the pre-prepared phase A1 is gradually added while continuously stirring to ensure thorough mixing. Then, the prepared phase B is added sequentially to the above mixture, maintaining a constant temperature and stirring to ensure that all active ingredients are uniformly dispersed throughout the product system, forming a stable skincare composition 3.

[0342] The composition and proportions of composition 4 in Comparative Example 1:

[0343]

[0344]

[0345]

[0346] The preparation method of composition 4 in Comparative Example 1: The prepared phase A is heated to 85 degrees Celsius to ensure that all oily components are fully dissolved and evenly distributed.

[0347] Heat phase B to the same temperature and keep stirring until homogeneous, then set aside.

[0348] When phase A is cooled to below 45 degrees Celsius, the preheated phase B is slowly added to phase A and emulsified using a homogenizer to fully combine the oil and water phases and form a stable emulsion system.

[0349] At a suitable temperature, each of the highly effective active ingredients in the pre-prepared C phase is added to the emulsion system one by one, and the mixture is continuously stirred and dispersed evenly until the final product, composition 4, is obtained.

[0350] The specific usage method is as follows, referring to Example 1:

[0351] Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean and refreshed so that subsequent skincare products can be effectively absorbed.

[0352] Application of Composition 1 in Comparative Example 1: After cleansing, apply an appropriate amount of Composition 1 evenly to the entire face and neck, and gently massage until fully absorbed. This composition serves as the first step in basic moisturizing and repair, aiming to create a good hydrated base for the skin.

[0353] For application of Composition 2 as in Example 1: After Composition 1 is fully absorbed, immediately apply Composition 2 evenly to the entire face and neck area, and gently massage to help the ingredients penetrate. If using during the day, and planning to apply a sunscreen, the application step of Composition 2 can be omitted, and proceed directly to the next step.

[0354] Comparative Example 1: Targeted treatment with Composition 3: For wrinkles around the eyes, apply an appropriate amount of Composition 3 to the skin around the eyes, especially to areas with wrinkles, and massage gently until fully absorbed. Composition 3 is rich in anti-wrinkle and firming ingredients, which can specifically improve wrinkles.

[0355] For complete coverage with Composition 4 (as in Example 1): After Composition 3 is absorbed, apply an appropriate amount of Composition 4 evenly to the entire face and neck, and massage thoroughly again to promote absorption. As a comprehensive conditioning product, Composition 4 consolidates the effects of all preceding steps in the final step, providing continuous nourishment and protection.

[0356] According to the above application procedure, this skincare routine is performed once each in the morning and evening. According to actual needs, users can adjust the skincare steps in the daytime and mix and match flexibly to achieve the optimal skincare effect.

[0357] Comparative Example 2

[0358] In the present study, in order to verify the anti-aging and repairing effect of the skincare composition system involved in the present invention and the uniqueness of its synergistic effect, a commercially available product focusing on anti-aging and repairing functions was selected as Comparative Example 2. This product is the Revitalizing Repair Night Cream of a high-end brand under a French cosmetics enterprise, and its record number with the National Medical Products Administration of China is National Cosmetics Network Filing Import Character (Shanghai) 2020007753. Through consulting official public materials, the ingredients of this comparative product are known as follows: "Water", "Propylene Glycol", "Dimethicone", "Hydroxypropyl Tetrahydropyrantriol", "Cyclohexasiloxane", "Isohexadecane", "Glycerin", "Synthetic Wax", "Dimethicone PEG-10 / 15 Crosspolymer", "Aluminum Starch Octenylsuccinate", "Lauryl PEG-9 Polydimethylsiloxyethyl Dimethicone", "CI 77163", "Magnesium Sulfate", "Phenoxyethanol", "Ethylhexyl Hydroxystearate", "Acrylonitrile / Methyl Methacrylate / Vinylidene Chloride Copolymer", "Fragrance", "Disodium EDTA", "Vaccinium Myrtillus Fruit Extract", "Dipotassium Glycyrrhizinate", "Hyaluronic

[0359] The reasons for selecting this comparative example are mainly based on the following two points: 1. Its advertised efficacy is consistent with the skincare effect described in the present patent, that is, it focuses on improving skin aging and promoting skin barrier repair; 2. This product contains a high concentration of Hydroxypropyl Tetrahydropyrantriol, which is similar to the key ingredient of Composition 2 in the present invention, so it can be used as an effective reference object for evaluating whether the synergistic effect of the four compositions in the present invention has significant advantages.

[0360] By comparing the systematic skincare regimen of the present invention with the skincare effect of the comparative product, it will help to objectively evaluate and confirm the innovation and effectiveness of the present invention in resisting skin aging.

[0361] Comparative Example 3

[0362] In this study, in order to verify the anti-aging and repairing effect of the skin care composition system involved in the present invention and the uniqueness of its synergistic effect, a representative commercially available product focusing on such efficacy is selected as Comparative Example 3. This product is a concentrated repairing serum launched by a high-end brand under a large American beauty company, with the record number in the National Medical Products Administration of China being 国妆网备进字(沪)2020004812. Through in-depth review of the officially published product ingredient information, it is found that its main components include: ["Cyclopentasiloxane", "Algae Extract", "Glycerin", "Dimethicone", "Polysilicone-11", "Isononyl Isononanoate", "Dimethicone PEG-10 / 15 Crosspolymer", "Cyclohexasiloxane", "Caprylic / Capric Triglyceride", "Water", "Dimethicone Crosspolymer", "Linoleic Acid", "Tetraacetylphytosphingosine", "Cholesterol", "CITRUS AURANTIFOLIA Peel Extract", "LAVANDULA ANGUSTIFOLIA Oil", "TRITICUM VULGARE Fine Powder Lipids", "Denatured Alcohol", "Butylated Hydroxytoluene", "CRITHMUM MARITIMUM Extract", "Yeast Extract", "PRUNUS AMYGDALUS DULCIS Seed Coarse Meal", "SESAMUM INDICUM Seed Powder", "MEDICAGO SATIVA Seed Powder", "HELIANTHUS ANNUUS Seed Cake", "Dipropylene Glycol", "Phenoxyethanol", "ROSMARINUS OFFICINALIS Leaf Oil", "OCIMUM BASILICUM Oil", "LAVANDULA HYBRIDA Oil", "LAMINARIA OCHROLEUCA Extract", "EUCALYPTUS GLOBULUS Leaf Oil", "Tocopheryl Succinate", "SESAMUM INDICUM Seed Oil", "Calcium Gluconate", "Sodium Gluconate", "Sodium Citrate", "Magnesium Gluconate", "Copper Gluconate", "Niacin", "Zinc Gluconate", "Alteromonas Ferment Extract", "HELICHRYSUM ARENARIUM Extract", "Potassium Sorbate", "Tocopherol (Vitamin E)", "CHLORELLA VULGARIS Extract"]

[0363] The main reasons for selecting this control example are as follows: 1. The product's market positioning and claimed efficacy significantly align with the skincare goals described in this invention, namely, focusing on combating skin aging and strengthening the skin barrier function. 2. This product uses cyclopentamethoxysiloxane as its main base material and combines multiple active ingredients to achieve its anti-aging performance characteristics. This technical approach is significantly similar to the composition system in this invention, which uses high-concentration yeast fermentation products as the basic carrier and is composed of multiple active substances. Therefore, selecting this product as a control allows for an effective comparative evaluation of whether the synergistic effect among the four compositions in this invention has significant advantages and innovative value.

[0364] Compare with Example 4

[0365] In the present study, to verify the anti-aging and anti-wrinkle effects of the skincare composition system involved in the present invention and the uniqueness of its synergistic effect, a representative commercially available product focusing on such effects was selected as Comparative Example 4. This product is a polypeptide anti-wrinkle essence from a brand under a large domestic cosmetics enterprise, with the filing number issued by the National Medical Products Administration of China: Shanghai G Cosmetics Network Filing No. 2022020464; through in-depth access to the product ingredient information published officially, it is found that its main composition includes:["Water", "Butylene Glycol", "Niacinamide", "Glycerin", "Dimethicone", "Cyclopentasiloxane", "1,2-Pentanediol", "Bis-PEG-18 Methyl Ether Dimethyl Silane", "Methyl Gluceth-20", "Glycereth-26", "1,3-Propanediol", "Polymethylsilsesquioxane", "PEG-20 Methyl Glucose Sesquistearate", "Phenoxyethanol", "Octyldodecanol", "Ectoin", "Acrylates / C10-30 Alkanol Acrylate Crosspolymer", "Carnosine", "CENTELLA ASIATICA Extract", "Bifida Ferment Lysate", "Dipotassium Glycyrrhizinate", "Tocopheryl Acetate", "Retinyl Palmitate", "Tromethamine", "Octanoyl Hydroxamic Acid", "Disodium EDTA", "ALBIZIA JULIBRISSIN Bark Extract", "POLYGONUM CUSPIDATUM Root Extract", "(Daily Use) Flavor", "SCUTELLARIA BAICALENSIS Root Extract", "Carbomer", "Sodium Lactate", "TREMELLA FUCIFORMIS Extract", "Sodium Hyaluronate", "GLYCYRRHIZA GLABRA Root Extract", "CAMELLIA SINENSIS Leaf Extract", "Hydrolyzed Sodium Hyaluronate", "Polysorbate-20", "ROSMARINUS OFFICINALIS Leaf Extract", "CHAMOMILLA RECUTITA Flower Extract", "Butylated Hydroxytoluene", "Tocopherol (Vitamin E)", "Sodium Benzoate", "Palmitoyl Tripeptide-1", "Palmitoyl Tetrapeptide-7", "CI 47005", "PANAX GINSENG Root Extract", "Sodium Chloride", "Sodium Sulfate", "CI 14720"]

[0366] The main reasons for selecting this control example are as follows: 1. The product's market positioning and claimed efficacy significantly align with the skincare goals described in this invention, namely, focusing on combating skin aging and wrinkles. 2. This product uses a combination of ingredients similar to those in this invention, such as bifida ferment lysate, carnosine, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7, to achieve its anti-aging and anti-wrinkle effects. Therefore, selecting this product as a control allows for an effective comparative evaluation of whether the synergistic effect among the four compositions in this invention has significant advantages and innovative value.

[0367] Experimental Test 1: Evaluation of Human Efficacy Test 1

[0368] Experimental objective: To verify the anti-wrinkle effect of the systemic skincare compositions (compositions 1-4) of this invention in the absence of core ingredients by measuring the improvement rate of skin elasticity and the improvement rate of skin wrinkle depth. A comparative analysis was then conducted with a control group.

[0369] Examples 1-4 represent products formulated with four different compositions according to varying proportions of the core active ingredient. Control Example 1 is a baseline control product containing none of the core active ingredients mentioned in the patent, used to evaluate the improvement in efficacy of each example relative to Control Example 1.

[0370] Specifically as follows:

[0371] 1 Example 1 Full ingredients 2 Example 2 Remove yeast fermentation products 3 Example 3 Remove bosonicin 4 Example 4 Remove Bose and Bifida Ferment Lysate 5 Compare with Example 1 Remove Bosein, Bifida Ferment Lysate and peptides

[0372] Experimental steps and contents:

[0373] Experimental Groups:

[0374] Testing Methods: One hundred eligible volunteers, regardless of gender and aged 35 to 55, were recruited. Participants were randomly assigned to five different experimental groups, each using the corresponding example product and control product.

[0375] Selection criteria and exclusion conditions:

[0376] Participants must meet strict physical requirements to ensure the validity and reliability of the results. The following individuals are ineligible for inclusion: those who have taken antihistamines within the past week or used immunosuppressants within the past month; those who have used any anti-inflammatory medication on the tested skin area within the past two months; those with a history of untreated inflammatory skin disease; those diagnosed with insulin-dependent diabetes mellitus; those currently receiving treatment for asthma or other chronic respiratory diseases; those who have undergone chemotherapy for cancer within the past six months; those with immunodeficiency or autoimmune diseases; breastfeeding or pregnant women; those who have undergone bilateral mastectomy and axillary lymph node dissection; those whose results are affected by scarring, pigmentation, atrophy, port-wine stains, or other skin blemishes; participants in other clinical trials; those with highly sensitive constitutions; and those who are not participating voluntarily or are unable to follow the complete experimental protocol.

[0377] After all inclusion criteria were met, a half-face control design was used in the experiment. The test sample was applied to the left half of the subject's face three times a week, while the right half of the face served as a control, with the corresponding control sample applied. Skin elasticity was measured at weeks 1, 2, 3, and 4 after application.

[0378] The skin elasticity testing method uses a Cutometer skin elasticity tester. A constant negative pressure is applied in the vertical direction and then released. By measuring the displacement change of the skin over a specified time period, the R value, which characterizes skin elasticity, is calculated. The calculation formula is as follows: R = (Uf - Ua) / Uf

[0379] Where Ua represents the skin's rebound displacement after the negative pressure is removed (unit: millimeters, mm); Uf represents the skin's maximum tensile displacement when negative pressure is applied (unit: millimeters, mm).

[0380] Ua — The skin's rebound displacement after the negative pressure is removed, measured in millimeters (mm).

[0381] Uf — The maximum tensile displacement of the skin when negative pressure is applied, in millimeters (mm).

[0382] The R-value was directly acquired and calculated by the instrument during the test. Further analysis was conducted on the relative change rate of the R-value, Tx vs. T0, which represents the improvement rate of skin elasticity. This is the ratio of the R-value after week X to the initial R-value (week 0, i.e., before use). This relative value directly reflects the trend of skin elasticity changes over time; a larger Tx vs. T0 value indicates a more significant improvement in skin elasticity.

[0383] The improvement rate of skin wrinkles was tested using the Primos Pico 3D imaging analyzer. Primos Pico, a high-speed 3D skin imaging system developed based on digital microscopic stripe projection technology, utilizes optical principles to achieve three-dimensional visualization and analysis of the microscopic texture of the skin surface and the depth and distribution characteristics of wrinkles. Through high-precision digital capture and quantitative calculation of wrinkles around the eyes, a scientific basis was provided for evaluating the efficacy of skincare products in improving wrinkles.

[0384] The results of the skin elasticity improvement rate are shown in the table below.

[0385]

[0386] The results of the skin wrinkle improvement rate are shown in the table below.

[0387]

[0388] Based on the above comparative data, the following preliminary conclusions can be drawn:

[0389] The following conclusions can be drawn from this human efficacy testing and evaluation experiment:

[0390] Rationality of ingredient combination: Experimental results show that when all core ingredients (yeast ferment, Pro-Xylane, and Bifida Ferment Lysate) are present in Example 1, the improvement rates of skin elasticity and wrinkle depth reach the highest levels. This fully demonstrates that the present invention has rationally combined various active ingredients in the skincare product formulation design, enabling them to work together on the skin and exert the maximum anti-aging effect.

[0391] Effectiveness of synergistic effects: With the removal of some or all of the core ingredients, the performance of the products in each embodiment in improving skin elasticity and wrinkles gradually diminished. This phenomenon indicates that there is a significant synergistic effect among the core ingredients, and their combined use can more effectively improve skin elasticity and reduce wrinkle depth, verifying the synergistic effect of the components in the composition of the present invention.

[0392] Scientific validity of each formulation: Through comparative analysis of products with different ingredient combinations, we can see that products containing all the core ingredients exhibit the best anti-wrinkle and skin-firming performance, while products lacking these key ingredients show relatively weaker effects. This result confirms the scientific validity of the formulations in this invention, namely, that through careful selection and formulation of core active ingredients, it is possible to effectively target skin aging problems and provide targeted and efficient skincare solutions.

[0393] In conclusion, this study strongly demonstrates that the systemic skincare composition of the present invention exhibits significant advantages in terms of the rationality of ingredient combination, the effectiveness of synergistic efficacy, and the scientific nature of each composition formulation, providing a strong scientific basis and practical guidance for the future development of skincare products.

[0394] Experimental Test 2: Human Efficacy Test Evaluation 2

[0395] Experimental objective: To verify the synergistic effect of the systemic skin care compositions 1-4 of the present invention by measuring the improvement rate of skin elasticity and the improvement rate of skin wrinkle depth.

[0396] Examples 1-4 represent products formulated with four different compositions according to varying proportions of the core active ingredient. Control Example 1 is a baseline control product containing none of the core active ingredients mentioned in the patent, used to evaluate the efficacy improvements of each example relative to Control Example 1.

[0397]

[0398] Experimental steps and contents:

[0399] Experimental Groups:

[0400] Testing Methods: Eighty eligible volunteers, regardless of gender and aged 35 to 55, were recruited. Participants were randomly assigned to four different experimental groups, each using the corresponding example product and control product.

[0401] Inclusion and exclusion criteria: Same as those for Experiment 1.

[0402] After all inclusion criteria were met, a half-face control design was used in the experiment. The test sample was applied to the left half of the subject's face three times a week, while the right half of the face served as a control, with the corresponding control sample applied. Skin elasticity was measured at weeks 1, 2, 3, and 4 after application.

[0403] The skin elasticity testing method uses a Cutometer skin elasticity tester. A constant negative pressure is applied in the vertical direction and then released. By measuring the displacement change of the skin over a specified time period, the R value, which characterizes skin elasticity, is calculated. The calculation formula is as follows: R = (Uf - Ua) / Uf

[0404] Where Ua represents the skin's rebound displacement after the negative pressure is removed (unit: millimeters, mm); Uf represents the skin's maximum tensile displacement when negative pressure is applied (unit: millimeters, mm).

[0405] Ua — The skin's rebound displacement after the negative pressure is removed, measured in millimeters (mm).

[0406] Uf — The maximum tensile displacement of the skin when negative pressure is applied, in millimeters (mm).

[0407] The R-value was directly acquired and calculated by the instrument during the test. Further analysis was conducted on the relative change rate of the R-value, Tx vs. T0, which represents the improvement rate of skin elasticity. This is the ratio of the R-value after week X to the initial R-value (week 0, i.e., before use). This relative value directly reflects the trend of skin elasticity changes over time; a larger Tx vs. T0 value indicates a more significant improvement in skin elasticity.

[0408] The improvement rate of skin wrinkles was tested using the Primos Pico 3D imaging analyzer. Primos Pico, a high-speed 3D skin imaging system developed based on digital microscopic stripe projection technology, utilizes optical principles to achieve three-dimensional visualization and analysis of the microscopic texture of the skin surface and the depth and distribution characteristics of wrinkles. Through high-precision digital capture and quantitative calculation of wrinkles around the eyes, a scientific basis was provided for evaluating the efficacy of skincare products in improving wrinkles.

[0409] The results of the skin elasticity improvement rate are shown in the table below.

[0410]

[0411]

[0412] The results of the skin wrinkle improvement rate are shown in the table below.

[0413]

[0414] Based on the provided data on skin elasticity improvement rate and skin wrinkle depth improvement rate, we can conduct the following analysis:

[0415] Analysis of skin elasticity improvement rate:

[0416] Test group 6 showed the most significant improvement in skin elasticity at all time points (from 1 to 4 weeks of use), and its relative change rate of R value was consistently higher than that of other test groups.

[0417] After 4 weeks of use, the skin elasticity improvement rate in test group 6 was 29.98% compared to the initial state, while the improvement rates in other groups were: 23.34% in test group 7, 20.08% in test group 8, and 21.74% in test group 9.

[0418] Conclusion: The skincare composition in test group 6 showed the strongest skin elasticity-enhancing effect, which supports the composition's good synergistic effect in improving skin elasticity.

[0419] Analysis of the improvement rate of skin wrinkle depth:

[0420] Similarly, in terms of wrinkle depth improvement, test group 6 also showed a trend of being superior to other groups.

[0421] After 4 weeks of use, the wrinkle depth improvement rate in test group 6 reached 36.05%, which was much higher than that in test group 7 (33.59%), test group 8 (29.74%) and test group 9 (31.56%).

[0422] Conclusion: Test group 6 showed the most significant improvement in the depth of skin wrinkles, further validating the superior performance of this systematic skin care composition in reducing wrinkles.

[0423] In summary, the experimental data show that the skincare composition in Example 1 (Test Group 6) not only performs best in improving skin elasticity, but also has the most significant effect in reducing the depth of skin wrinkles, thus strongly demonstrating the synergistic effect of the systemic skincare compositions 1-4 of the present invention.

[0424] Experimental Test 3: Human Skin Patch Test

[0425] Test samples: Compositions 1, 2, 3 and 4 from Example 1.

[0426] Test method:

[0427] To conduct a rigorous human skin tolerance assessment of compositions 1, 2, 3 and 4 in Example 1, this study strictly screened and recruited 80 volunteers who met the established criteria (see Experimental Test 1 for details) to ensure that the subjects' health status and skin type met the experimental requirements.

[0428] The 80 subjects were randomly divided into four groups of 20 each, and each group underwent an independent patch test on one of the four different compositions in Example 1.

[0429] 10 Composition 1 in Example 1 11 Composition 2 in Example 1 12 Composition 3 in Example 1 13 Composition 4 in Example 1

[0430] During the experiment, each test sample was first diluted with purified water to 1 / 100 of its original concentration according to the prescribed procedure, and purified water was set up as a control group. In the experimental operation, the researchers followed the aseptic operation procedure, accurately measured 0.020 ml of each test sample, and injected it evenly into the small chamber of the spot tester.

[0431] Subsequently, a suitable test area was selected on the flexor side of the subject's forearm, ensuring the skin in that area was clean and dry. Researchers carefully unwrapped the patch applicator packaging, attached the patch applicator (pre-loaded with the test sample or control solution) to the subject's skin, and gently pressed it with their palms to ensure close and even contact with the skin, guaranteeing the effective contact area between the sample and skin and the consistency of the reaction.

[0432] The skin patch applicator was left on for 24 hours after application to simulate the longest possible contact time of the product under normal use conditions. Subsequently, at key time points after the skin patch was removed—30 minutes, 24 hours, and 48 hours—professionals carefully observed and recorded the local reactions of the subjects' skin according to a pre-set standard evaluation system, and analyzed and evaluated the safety and tolerability of each skin care composition to human skin.

[0433]

[0434] The experimental data results are shown in the table below.

[0435]

[0436] According to the experimental data, all test groups (compositions 1, 2, 3 and 4) had a skin reaction score of 0 after the patch test, that is, no adverse reactions such as erythema, edema, infiltration or herpes occurred. This indicates that the subjects showed good skin tolerance to the four compositions in Example 1 after 24 hours of patch application, and no obvious irritation or allergic reactions occurred.

[0437] In conclusion, the results of this human skin patch test strongly demonstrate that compositions 1, 2, 3, and 4 in Example 1 have high safety and good skin tolerance, making them suitable for use on all types of skin. This provides a scientific basis for the development and marketing of subsequent products.

[0438] Although the present invention has been fully described and disclosed above with reference to specific embodiments, it should be understood that the present invention is not limited to these specific embodiments. Those skilled in the art, after reading this disclosure, will be fully capable of making various reasonable changes, optimizations, or equivalent substitutions to the present invention within the spirit and scope of the appended claims, tailored to different application needs and technical environments. All such improvements and modifications based on the basic concept and innovative spirit of the present invention should also be considered to fall within the scope of protection claimed by the present invention and be equally protected by law.

Claims

1. A synergistic, multi-dimensional anti-wrinkle skincare system, characterized in that: This system is designed for anti-wrinkle purposes and includes four customized compositions 1-4. Each composition contains specific active ingredients and preparation processes, and uses yeast fermentation products as the base solvent, with a concentration of 20-60%. Composition 1: Components and addition ratio range: Phase A is a water-soluble matrix: deionized water: 10%-20%; Phase A1 is a polyol moisturizing system: 1,3-propanediol: 0.5%-2%; glycerin: 1%-8%; Butanediol: 0.1%-0.5%; 1,2-Hexanediol: 0.2%-2%; Phase B is a bioactive complex: yeast fermentation products: 25%-40%; cucumber fruit extract: 1%-5%; *Ichthyophthirius multifiliis* extract: 1%-5%; trehalose or *Bifida ferment lysate* fermentation product lysate: 1%-5%; Composition 2: Components: Phase A: Glycerin: 1%-2%; Inulin lauryl carbamate: 0.5%-1%; Squalane: 0.1%-1%; Phase B: Fermented Tremella fuciformis water: 20%-40%; 1,3-propanediol: 1%-10%; Glyceryl ether-26: 1%-5%; Sodium hyaluronate: 0.03%-0.1%; Ganoderma lucidum extract: 1%-5%; Phase B1: 1,2-Hexanediol: 0.2%-1%; p-Hydroxyacetophenone: 0.1%-0.5%; Xanthan gum: 0.01%-0.1%; Phase C: Complex containing 35% hydroxypropyl tetrahydropyrantriol: 20%-30%; Complex containing 75% Bifida ferment lysate: 10%-20%; Multiple peptide complexes; Composition 3: Components and addition ratio range: Phase A: Yeast fermentation products: 20-40%; Deionized water: 10-30%; Phase A1: Disodium EDTA: 0.01-0.05%; Allantoin: 0.1-0.3%; 1,3-Propylene Glycol: 1-3%; Dipropylene Glycol: 1-3%; 1,2-Hexanediol: 1-3%; Sodium Hyaluronate: 0.01-0.05%; Phase B: Compositions containing palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7: MATRIXYL 3000 MBAL: 8-10%; polydimethylsiloxane: 10-25%; composite containing 35% hydroxypropyl tetrahydropyranotriol: 5-10%; sodium acrylate / sodium acryloyl dimethyl taurate copolymer: 2-5%; Bifida ferment lysate: 2-5%; Composition containing acetyl hexapeptide-8: WKPep® Argine500 plus Argine500Plus: 2%-5%; Composition containing palmitoyl tripeptide-5: SYN®-COLL: 1-2%; Composition containing arginine / lysine peptides: 1-5%; Aloe vera leaf juice: 1-5%; *Porphyra yezoensis* extract: 1-5%; hydroxydecyl ubiquinone: 0.1-0.5%; *Stephania tetrandra* extract: 1-5%; *Echinacea purpurea* extract: 1-5%; arginine / lysine peptide powder: 0.1-0.5%; apple fruit extract: 1-5%; hydrolyzed collagen: 0.1-0.5%. Caffeine: 0.1-0.5%; Composition 4: Components and addition ratio range: Phase A: C12-20 PEG-8 ester: 6%; Glycerin 0.5-1%; Tris(octyldodecanoyl)citrate: 3-6%; Tocopherol Vitamin E: 1-5%; Caprylic / Capric triglyceride: 3-8%; Phenyl polytrimethylsiloxane: 1-3%; Sunflower seed oil: 0.1-0.5%; Cetearyl alcohol: 1-3%; Polydimethylsiloxane: 1-5%; Tocopherol acetate: 0.1-1%; Phase B: Deionized water: 15-30%; Isopentyl glycol: 5-8%; Potassium cetyl phosphate: 0.1-0.3%; Tremella fuciformis fermentation water: 20-45%; Ammonium acryloyldimethyl taurate / VP copolymer: 0.5-1%; Sodium hyaluronate: 0.05-0.1%; 1,2-hexanediol: 0.2-1%; p-hydroxyacetophenone: 0.2-0.4%; Black goji berry puree: 2-5%; Phase C: 1%-2% of a complex containing 35% hydroxypropyl tetrahydropyrantriol; 0.5-1% of a composition containing arginine / lysine peptides; 1-5% of a complex fermented water containing grape, pomegranate, and birch polypore; 1-5% of a barley and tremella fuciformis puree; 1-5% of a purslane puree; 1-5% of an ergothioneine and pine mushroom extract; and 1-5% of a purple algae extract. Bisabolol: 0.1-1%; Tetrahydromethylpyrimidine carboxylic acid: 0.15-1%; Purple Duo: 0.15-1%; Plant-based soothing agent: 0.1-1%; Combination of ingredients containing alanine, glutamic acid, aspartic acid, nicotinic acid hexyl ester, and glucose: 0.15-1%; Combination of sugar isomers PentavitiN®: 1-5%; Cucumber fresh water: 1-5%; Caffeine: 0.2-1%.

2. A method for preparing a synergistic, multi-dimensional anti-wrinkle skincare system, characterized in that: The preparation method is used to prepare a synergistic multi-dimensional anti-wrinkle skin care system according to claim 1, wherein the preparation method of composition 1 is to heat and mix phase A and phase A1 at 85°C, cool to 45°C and then add phase B active ingredients to improve the moisture content of the stratum corneum and overall skin health.

3. The method for preparing a synergistic multi-dimensional anti-wrinkle skincare system according to claim 2, characterized in that: The composition 2 of claim 1 involves stepwise heating and fusing phases A, B, and B1, and adding peptide complexes and other active substances from phase C when the temperature is lowered to 45°C, in order to promote cell renewal and enhance the elasticity of the dermis.

4. The method for preparing a synergistic multi-dimensional anti-wrinkle skincare system according to claim 2, characterized in that: The composition 3 of claim 1 is specifically designed for eye wrinkle care. After dissolving and dispersing phase A and phase A1 at high temperature, phase B active ingredients are added at low temperature. It contains a specific ratio of peptides and moisturizers to combat dynamic and static wrinkles around the eyes.

5. The method for preparing a synergistic multi-dimensional anti-wrinkle skincare system according to claim 2, characterized in that: The composition 4 in claim 1 utilizes oil-water emulsification technology to combine phase A oil components and phase B water-soluble components at a specific temperature, and then adds phase C repairing and moisturizing components, stirring evenly at below 45°C to form an overall skin care formula with enhanced anti-wrinkle effects.

6. A method of using a synergistic, multi-dimensional anti-wrinkle skincare system for non-therapeutic purposes, characterized in that: The method of use is based on the synergistic multi-dimensional anti-wrinkle skincare system described in claim 1, and specifically includes the following steps: (1) Cleansing stage: First, thoroughly cleanse your face to ensure that your skin is clean so that subsequent products can be effectively absorbed; (2) Application of Composition 1: After cleansing, apply an appropriate amount of Composition 1 evenly to the face and neck, and massage until fully absorbed to achieve basic moisturizing and repairing functions. (3) Applying Composition 2: After Composition 1 has been fully absorbed, apply and massage Composition 2 onto the entire face and neck; if using during the day and planning to apply sunscreen, this step can be skipped; (4) Targeted eye care: Take an appropriate amount of Composition 3 and apply it specifically to the eye area. Apply it carefully to the wrinkled area and massage it in until absorbed. Use the anti-wrinkle and firming ingredients to improve the condition of wrinkles. (5) Full coverage of composition 4: After composition 3 is fully absorbed, apply an appropriate amount of composition 4 evenly to the face and neck and massage. This composition acts as a comprehensive conditioning agent to consolidate the previous skin care effect and provide continuous nourishment and protection. (6) Follow the skincare routine described once in the morning and once in the evening, and adjust the daytime skincare steps according to actual needs. Use the combination of products flexibly to ensure the best skincare effect.

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