A plant essential oil formula for treating rhinitis and its preparation method

Abstract

The present invention provides a plant essential oil formula for treating rhinitis and a preparation method thereof. The raw materials include Ficus hirta Vahl, ambergris, agarwood, elemi and camellia oil. The plant essential oil prepared by the present invention has strong bactericidal, nasal orifice unblocking and lung function enhancing effects, achieving the purpose of treating rhinitis.

Description

Technical Field

[0001] The present invention relates to the technical field of drugs for treating rhinitis, and particularly relates to a plant essential oil formula for treating rhinitis and a preparation method thereof. Background Art

[0002] Rhinitis refers to the inflammation of the nasal mucosa and submucosa. It is manifested as congestion or edema, and the symptoms mainly include nasal congestion, runny nose, sneezing, headache, dizziness, etc. The characteristic of nasal congestion is intermittent. Nasal congestion is alleviated during the day, in hot weather, during labor or exercise, while it is aggravated at night, when sitting still or in the cold. Another characteristic of nasal congestion is alternation. For example, when lying on the side, the nasal cavity on the lower side is blocked, and the upper nasal cavity has good ventilation. Due to nasal congestion, there may be symptoms such as occasional loss of smell, headache, dizziness, and a blocked nasal voice when speaking. In severe cases of rhinitis, it will affect sleep.

[0003] Chinese Patent CN114699375A, a traditional Chinese medicine preparation for treating rhinitis and its using method, the raw materials include 13g of Xanthium sibiricum (fried until charred and thorns removed), 9g of Magnolia liliflora, 10g of Angelica dahurica, 20g of Mentha haplocalyx, 10g of Prunella vulgaris, 3g of Borneol, 3g of Asarum sieboldii, 10g of Centipeda minima, 6g of Cinnamomum cassia, 3g of Gleditsia sinensis and 6g of Arctium lappa. The above 11 kinds of medicinal materials are ground into extremely fine powder, and it takes three weeks for this preparation to take effect. CN107496743B, a traditional Chinese medicine composition for treating chronic rhinitis and allergic rhinitis and its preparation method and application, the traditional Chinese medicine composition includes, by weight, 15 - 25 parts of Centipeda minima, 45 - 75 parts of Plantago asiatica, 45 - 75 parts of Taraxacum mongolicum, 35 - 65 parts of Phragmites communis rhizome, and 15 - 25 parts of Glycyrrhiza uralensis. The traditional Chinese medicine composition of the present invention is composed of multiple components, and these components cooperate with each other and synergistically promote, and can effectively inhibit various viruses, bacteria and molds in the nasal cavity. The treatment period of this traditional Chinese medicine composition is one month. Therefore, there is a need for a plant essential oil that can take effect quickly and rapidly relieve the discomfort symptoms brought by rhinitis. Summary of the Invention

[0004] In view of this, the present invention provides a plant essential oil formula for treating rhinitis and a preparation method thereof to solve the above problems.

[0005] The technical solution of the present invention is realized as follows:

[0006] A plant essential oil formula for treating rhinitis, the raw materials include Ficus hirta, ambergris, agarwood, elemi and chaulmoogra oil.

[0007] Further, the preparation method of the plant essential oil formula for treating rhinitis includes the following steps:

[0008] (1) Mix ambergris with water, heat it, take the upper layer of oil, refine it to obtain ambergris oil and ambergris extract;

[0009] (2) Extract agarwood using the steam distillation method to obtain agarwood essential oil, agarwood filter residue, and agarwood extract;

[0010] (3) Mix elemi pieces with water, carry out condensation reflux to obtain elemi oil and elemi extract;

[0011] (4) Mix the agarwood filter residue with Ficus hirta, add the mixed extract, heat, filter, and concentrate the filtrate to obtain an extract;

[0012] Heat the chaulmoogra oil, and then add the extract, ambergris oil, and agarwood essential oil to obtain the finished product.

[0013] Further, in step (1), the mass ratio of ambergris to water is 1:8 - 10, heat to boiling, and maintain boiling for 1.8 - 2.2 h.

[0014] Further, in step (2), the extraction temperature of the steam distillation method is 98 - 100 °C, the extraction time is 100 - 120 min, and the mass ratio of agarwood to water in the material-liquid is 1:3 - 5.

[0015] Further, in step (3), the mass ratio of elemi pieces to water in the material-liquid is 1:0.9 - 1.1, and the condensation reflux is carried out at 95 - 100 °C for 2 - 3 h.

[0016] Further, in step (4), the mixed extract is prepared by mixing 500 - 800 g of ambergris extract, 1 - 1.5 kg of agarwood filtrate, and 1 - 1.5 kg of elemi extract per kilogram of the weight of Ficus hirta.

[0017] Further, in step (4), the mass ratio of the agarwood filter residue to Ficus hirta is 0.1 - 0.15:1.

[0018] Further, in step (4), the heating is carried out at 95 - 105 °C for 4 - 6 h, and in step (5), the heating temperature of the chaulmoogra oil is 45 - 65 °C.

[0019] Further, in step (5), 0.2 - 50 g of the extract, 3 - 5 g of agarwood essential oil, and 10 - 15 g of ambergris oil are added per kilogram of chaulmoogra oil and mixed to obtain the finished product.

[0020] Further, in step (3), the Ficus hirta is the dry root powder of Ficus hirta.

[0021] Further, the formation age of the agarwood is more than 10 years.

[0022] Further, the method of using the plant essential oil is to heat 1 kg of Hainan shanyou oil, gradually add 50 g of extract, fully dissolve it, then gradually add 3 - 5 g of agarwood essential oil and 10 - 15 g of ambergris oil, mix evenly, and take 0.5 ml each time and put it into a fumigator and add 50 ml of clear water to fumigate the nasal cavity.

[0023] Further, the method of using the plant essential oil is to add 3 - 5 g of agarwood essential oil and 10 - 15 g of ambergris oil to 1 kg of Hainan shanyou oil, mix evenly to obtain a mixed oil. Take 0.2 g of extract and dissolve it in 50 ml of clear water, then add 0.5 ml of the mixed oil and use a fumigator to fumigate the nasal cavity.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] The ambergris of the present invention has the effects of promoting qi and activating blood circulation, dissipating stasis and relieving pain, promoting diuresis and draining stranguria, has strong fragrance retention and penetration ability, and can promote the absorption of agarwood by the body when combined with agarwood. Agarwood has the effects of promoting qi and relieving pain, warming the middle-jiao and stopping vomiting, and assisting qi in descending to relieve asthma, and has good ventilation ability. The functions of elemol essential oil: relieving pain, antiviral, bactericidal, having the effect of balm, resolving phlegm, killing molds, tonifying the body, and treating wounds. Ficus hirta: It has the functions of strengthening the spleen and resolving dampness, promoting qi and resolving phlegm, relaxing muscles and activating collaterals, and tonifying qi and consolidating the exterior. Hainan shanyou oil contains various nutritional elements such as protein, vitamin C, vitamin E, calcium, and iron, and Hainan shanyou oil can supplement various nutrients for the body. The plant essential oil prepared by the present invention has strong bactericidal, nasal orifice unblocking, and lung function enhancing effects, achieving the purpose of treating rhinitis.

[0026] The plant essential oil prepared by the present invention has good use effects. Using it before going to bed can significantly reduce the snoring frequency and prolong the sleep time. Using it once in the morning can reduce the discomfort caused by rhinitis. Using it for 7 - 14 days, the rhinitis symptoms will completely disappear. Specific Embodiments

[0027] To better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0028] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0029] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can all be obtained from commercial channels.

[0030] Example 1

[0031] The preparation method of the plant essential oil formula for treating rhinitis includes the following steps:

[0032] (1) Mix ambergris and water at a material-liquid ratio of 1:9, heat to boiling, maintain the boiling state for 2 hours, cool at room temperature, let stand for about 3 hours, then take the upper-layer grease, and use an appropriate amount of anhydrous sodium sulfate to remove the water in the grease to obtain ambergris oil and ambergris extract for standby.

[0033] (2) Add agarwood with a formation age of more than 10 years to water at a material-liquid mass ratio of 1:4, and extract by steam distillation for 100 - 120 min. Separate to obtain agarwood essential oil, agarwood filter residue, and agarwood extract for standby.

[0034] (3) Mix elemi pieces and water at a material-liquid mass ratio of 1:1, condense and reflux and boil for 2 hours, and let stand for 2 hours to obtain elemi extract for standby.

[0035] (4) Mix 500 - 800 g of ambergris extract, 1 - 1.5 kg of agarwood filtrate, and 1 - 1.5 kg of elemi extract to obtain a mixed extract.

[0036] (5) Mix 130 g of agarwood filter residue and 1 kg of dry root powder of Ficus hirta, add the mixed extract and heat, filter, and concentrate the filtrate to obtain an extract.

[0037] Comparative Example 1

[0038] The preparation method of a plant essential oil formula for treating rhinitis includes the following steps:

[0039] (1) Add agarwood with a formation age of more than 10 years to water at a material-liquid mass ratio of 1:4, and extract by steam distillation for 100 - 120 min. Separate to obtain agarwood essential oil, agarwood filter residue, and agarwood extract for standby.

[0040] (2) Mix elemi pieces and water at a material-liquid mass ratio of 1:1, condense and reflux and boil for 2 hours, and let stand for 2 hours to obtain elemi extract for standby.

[0041] (3) Mix 1 - 1.5 kg of agarwood filtrate and 1 - 1.5 kg of elemi extract to obtain a mixed extract.

[0042] (4) Mix 130 g of agarwood filter residue and 1 kg of dry root powder of Ficus hirta, add the mixed extract and heat, filter, and concentrate the filtrate to obtain an extract.

[0043] Comparative Example 2

[0044] The preparation method of a plant essential oil formula for treating rhinitis includes the following steps:

[0045] (1) Mix ambergris and water at a material-liquid ratio of 1:9, heat to boiling, maintain the boiling state for 2 hours, cool at room temperature, let stand for about 3 hours, then take the upper-layer grease, and use an appropriate amount of anhydrous sodium sulfate to remove the moisture in the grease to obtain ambergris oil and ambergris extract for standby.

[0046] (2) Add agarwood with a formation age of more than 10 years to water at a material-liquid mass ratio of 1:4, and use the steam distillation method to extract for 100 - 120 minutes, then separate to obtain agarwood essential oil, agarwood filter residue, and agarwood extract for standby.

[0047] (3) Mix elemicin blocks and water at a material-liquid mass ratio of 1:1, carry out condensation reflux and boil for 2 hours, and let stand for 2 hours to obtain elemicin extract for standby.

[0048] (5) Mix 130 g of agarwood filter residue and 1 kg of dry root powder of Ficus hirta, add water and heat. The material-liquid ratio of water to dry root powder of Ficus hirta is 3:1. Filter, and take the filtrate and concentrate it to obtain an extract.

[0049] The usage methods of Example 1 and Comparative Examples 1 - 2 are as follows: Heat 1 kg of Hainan wild oil, gradually add 50 g of the extract, dissolve it thoroughly, then gradually add 4 g of agarwood essential oil and 13 g of ambergris oil, mix evenly to obtain plant essential oil. Each time, take 0.5 mL and put it into a fumigator, add 50 mL of clear water and fumigate the nasal cavity.

[0050] Comparative Example 3

[0051] On the basis of Example 1, change the ratio of the plant essential oil. Specifically: Heat 1 kg of Hainan wild oil, gradually add 50 g of the extract, dissolve it thoroughly, then gradually add 5 g of agarwood essential oil and 5 g of ambergris oil, mix evenly to obtain plant essential oil. Each time, take 0.5 mL and put it into a fumigator, add 50 mL of clear water and fumigate the nasal cavity.

[0052] Test Example 1 Antibacterial Test

[0053] Conduct antibacterial tests on the plant essential oils prepared in Example 1 and Comparative Examples 1 - 3. The test bacteria are Candida albicans, Escherichia coli, and Staphylococcus aureus. The bacterial strains are sourced from the Guangdong Food Microbiology Safety Engineering Technology Research and Development Center. The culture medium is selected as nutrient agar medium. Refer to the test methods for product bactericidal performance, antibacterial performance, and stability in Appendix C of GB15979 - 2002 "Hygiene Standards for Disposable Sanitary Products". The usage concentration of the plant essential oil is 10 μg / mL. Detect the inhibitory effects of the plant essential oils prepared in Example 1 and Comparative Examples 1 - 3 on the test bacteria after 1 hour.

[0054] Name Candida albicans (%) Escherichia coli (%) Inhibitory rate against Staphylococcus aureus (%) Example 1 99.8 99.7 100 Comparative Example 1 96.1 92.3 98.4 Comparative Example 2 97.7 90.6 97.3 Comparative Example 3 98.4 95.8 99.1

[0055] The test results show that the plant essential oil prepared by the present invention has good inhibitory effects on Candida albicans, Escherichia coli and Staphylococcus aureus. Referring to the experimental results of Comparative Example 1, adding ambergris in the present invention can improve the inhibitory effects of the plant essential oil on Candida albicans, Escherichia coli and Staphylococcus aureus, especially on Escherichia coli. In Comparative Example 2, water is used to replace the mixed extract. In the present invention, the mixed extract, agarwood residue and dried root powder of Ficus hirta are used for secondary extraction, which can extract more effective components for inhibiting pathogenic bacteria. In Comparative Example 3, the raw material ratio in the plant essential oil is changed, resulting in a decrease in the inhibitory effects of the plant essential oil on Candida albicans, Escherichia coli and Staphylococcus aureus. The reasonable raw material ratio of the plant essential oil in the present invention improves the bacteriostatic effect of the plant essential oil.

[0056] Test Example 2

[0057] Each rat in the model group was intraperitoneally injected with 1 mL of a suspension prepared by using 30 mg of OVA (albumin) as an antigen, 3 g of aluminum oxide powder as an adjuvant, and 100 mL of physiological saline as a solvent. The normal group of rats was intraperitoneally injected, and the injection was performed once every other day. The above process could complete the basic sensitization stage after 7 times. After the basic sensitization of the AR model rats was completed, nasal challenge was started: except for the rats in the normal group, 2% OVA physiological saline was prepared and dropped into both nasal cavities, 0.05 mL for each side, once a day for a total of 7 times. The normal group of rats was instilled with 0.05 mL of physiological saline into each nostril, once a day for a total of 7 times. The successfully modeled mice were divided into a model group and an experimental group. There were 5 mice in each of the normal group, the model group and the experimental group, a total of 25 mice. The experimental group of mice was tested with the plant essential oils of Example 1 and Comparative Examples 1-3, and the dosage of each mouse was 0.5 g / kg. Kits for IL-4, OVA-sLgE, and IFN-γ were used to detect the levels of IL-4, OVA-sLgE, and IFN-γ in the rat serum.

[0058] Name IL-4 (pg / mL) OVA-sLgE (ng / mL) IFN-γ (pg / mL) Example 1 153.67 29.55 3325.12 Comparative Example 1 159.34 31.37 3127.41 Comparative Example 2 156.72 30.89 3187.67 Comparative Example 3 161.57 30.24 3254.49 Blank group 165.72 28.41 3428.83 Model group 186.53 35.84 2681.64

[0059] The test results show that the plant essential oil prepared by the present invention has the effects of promoting vasodilation, enhancing capillary permeability and increasing glandular secretion, and further achieves the effect of treating rhinitis. The test results of Comparative Example 1 show that ambergris has the effects of promoting qi and activating blood circulation, dissipating stasis and relieving pain, promoting diuresis and relieving stranguria, and has strong fragrance retention and penetration ability. Combining with agarwood can promote the absorption of agarwood by the body. The experimental results of Comparative Example 2 show that using the combined extract for secondary extraction in the present invention can improve the use effect of the plant essential oil. The test results of Comparative Example 3 show that the reasonable raw material ratio of the present invention can improve the use effect of the plant essential oil.

[0060] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A plant essential oil product for treating rhinitis, characterized in that: The raw materials are five-fingered peach, ambergris, agarwood, elemi blocks and mountain grapefruit oil; The method for preparing the plant essential oil product for treating rhinitis comprises the following steps: (1) Mixing ambergris and water, heating them, taking the upper layer of fat, refining it, and obtaining ambergris oil and ambergris extract; (2) extracting agarwood by steam distillation to obtain agarwood essential oil, agarwood residue and agarwood extract; (3) mixing elemi blocks with water, condensing and refluxing, and obtaining elemi oil and elemi extract; (4) Mixing the agarwood residue and the quinquefolia, adding the mixed extract, heating, filtering, and concentrating the filtrate to obtain an extract; (5) heating grapefruit oil and adding extract, ambergris oil and agarwood essential oil to obtain a finished product; In step (1), the mass ratio of ambergris to water is 1:8-10, and the mixture is heated to boiling and kept boiling for 1.8-2.2 hours; In step (2), the steam distillation extraction temperature is 98-100°C, the extraction time is 100-120min, and the mass ratio of agarwood to water is 1:3-5; In step (3), the mass ratio of the elemi block to water is 1:0.9-1.1, and the condensation reflux is refluxed at 95-100° C. for 2-3 hours; In step (4), the mixed extract is prepared by adding 500-800 g of ambergris extract, 1-1.5 kg of agarwood filtrate and 1-1.5 kg of elemi extract per kilogram of quinquefolia. In step (4), the mass ratio of the agarwood residue to the five-finger peach is 0.1-0.15:1; In step (5), 0.2-50 g of extract, 3-5 g of agarwood essential oil and 10-15 g of ambergris oil are added to each kilogram of grapefruit oil and mixed to obtain a finished product.

2. The plant essential oil product for treating rhinitis according to claim 1, characterized in that: In step (4), the heating is carried out at 95-105°C for 4-6 hours. In step (5), the heating temperature of the grapefruit oil is 45-65°C.

3. The plant essential oil product for treating rhinitis according to claim 1, characterized in that: In step (4), the quinquefolius is quinquefolius dried root powder.