A method for rapid propagation of Cinnamomum camphora seedlings by integrated cuttings

Abstract

The invention discloses a method for rapid propagation of camphor tree seedlings by integrated cuttings, and belongs to the technical field of camphor tree cuttings. The method comprises the steps of land preparation, cuttings collection and processing, cuttings, and later management and maintenance. 2-3 year-old camphor tree seedling branches are used as cuttings, which are cut into a specific matrix. This shortens the rooting time of the camphor tree cuttings and solves the problem of cutting shortage in the current camphor tree industry development. Furthermore, while collecting the cuttings, the seedlings are pruned to promote the formation of multi-branched seedlings, thereby reducing the labor cost of pruning the seedlings after planting, and better improving the camphor tree yield. The method for rapid propagation of camphor tree cuttings provided by the invention has good application prospects in the field of camphor tree breeding.

Description

Technical Field

[0001] The invention relates to the technical field of Cinnamomum camphora cuttings, and in particular to a Cinnamomum camphora seedling propagation integrated cuttings and rapid propagation method. Background Art

[0002] Cinnamomum camphora, a species of Litsea cubeba in the Lauraceae family, is a variant of Litsea cubeba. Eagle tea, made from fresh leaves of Cinnamomum camphora, has been a popular beverage since ancient times. Eagle tea boasts health benefits such as thirst-quenching, heat-clearing and detoxification. Its natural, pollution-free, and unique flavor make it highly sought after and offers both excellent drinking and economic value.

[0003] The traditional propagation methods for Cinnamomum camphora mainly include seed propagation and cutting propagation. Cinnamomum camphora is dioecious, and only a very small number of mature trees can produce seeds. Due to the limited number of seeds, large-scale seedling propagation cannot be carried out using seeds. In addition, Cinnamomum camphora seeds contain substances that inhibit rooting, resulting in a low natural seedling rate and slow initial growth of seedlings. Cutting propagation is simple, has low variability, and can better maintain the quality and characteristics of the mother plant. However, traditional cutting propagation requires cuttings to be collected from high-quality mother trees. Due to the demand for tea, there is a shortage of cuttings for Cinnamomum camphora cuttings. In addition, the rooting time of Cinnamomum camphora cuttings using traditional cutting propagation methods is long, further limiting the development of the eagle tea industry. Summary of the Invention

[0004] Based on the above technical problems, the purpose of the present invention is to provide a method for rapid propagation of Cinnamomum camphora seedlings by cuttings, to solve the current problems of shortage of Cinnamomum camphora cuttings resources and long rooting time of cuttings, so as to promote the further development of the eagle tea industry.

[0005] The present invention solves the above technical problems through the following technical means:

[0006] A method for rapid propagation of Cinnamomum camphora seedlings by cuttings and propagation, the method being as follows:

[0007] (1) Land preparation: plough the soil and crush the soil clods. After drying for 1-2 days, spray the soil with 0.1% potassium permanganate solution to disinfect the soil. Spread the cutting medium on the surface of the disinfected soil.

[0008] (2) Cutting collection and processing: Select 2-3 year old Cinnamomum camphora seedlings, cut off the upper part of the trunk and trim it to a length of 4-7 cm to obtain cuttings, disinfect the cut end and soak it in rooting solution for 30-60 minutes to obtain pre-treated cuttings;

[0009] (3) Cutting: In September and October, the pre-treated cuttings are cut into the soil after the cutting medium is spread flat, and then the maintenance is carried out according to the conventional method.

[0010] In the traditional cutting method of Cinnamomum camphora, cuttings are usually collected from old trees for cutting propagation. However, due to the demand for tea picking, there are few cuttings available for cutting propagation on old trees, and the rooting time of cuttings is long. In research practice, the inventors found that using 2-3 year old seedling branches as cuttings can greatly shorten the rooting time and increase the survival rate, which can effectively improve the efficiency of Cinnamomum camphora cutting propagation. In addition, in the conventional production process, continuous pruning is required after the leopard skin camphor tree is planted in the ground to promote the branching of the seedlings to increase the yield. The present invention collects spikelets from the seedlings in the seedling stage to promote the branching of the seedlings to form multi-branched seedlings. The number of branches of conventional seedlings or cuttings is mostly 1-2, while the number of branches of the seedlings after the spikelets are collected in the present invention can be increased to 4-8. The multi-branched seedlings formed can greatly increase the tea yield of the leopard skin camphor tree, reduce the pruning operations after the seedlings are planted in the ground, shorten the production period, and carry out the cutting propagation and seedling cultivation of the leopard skin camphor tree at the same time, effectively reducing the production cost of the leopard skin camphor tree.

[0011] Furthermore, the cutting matrix preparation method is as follows:

[0012] A: Prepare a 3wt% sodium alginate solution, add n-octanol, mix, and heat to 70-75°C with stirring for 1-2 hours. After the reaction is complete, cool to room temperature and add 2,4-di-tert-butylphenol and vermiculite, mixing evenly. Then, simultaneously add ethyl glycolate and 10wt% calcium chloride solution, stirring to form a gel. After drying, grind into granules to obtain gel-coated primary particles.

[0013] B: Separately prepare a 3wt% sodium alginate solution, add 2,4-di-tert-butylphenol, stir well, and adjust the pH to 4-5. Heat to 50-60°C, add the gel to initially coat the particles, and mix well. Immediately add a 10wt% calcium chloride solution and stir to form a gel. Let stand for 1-2 hours, then dry and grind into granules to obtain gel-coated particles.

[0014] C: Mix the excavated detoxified soil with coconut bran and humic acid, adjust the moisture content and pH, add the fully water-absorbing gel secondary coating particles, and mix well to obtain the cutting medium.

[0015] During indoor experiments, the inventors found that collecting 2-3 year old seedling branches as cuttings can significantly shorten the rooting time and increase the survival rate. However, when cuttings are carried out in the wild, because the cuttings collected from 2-3 year old seedlings are relatively young, the stem tissue is not fully developed, the resistance is poor, and the sensitivity to the environment is high, the uncontrollable nature of the wild environment increases the mortality rate of leopard skin camphor after cutting in the wild, and greenhouse cuttings greatly increase the breeding cost. In addition, the leopard skin camphor cuttings have no roots to absorb water in the early stages of cutting, and the demand for water is high. As the cutting callus tissue forms and gradually takes root, the demand for water gradually decreases. In the later stage, excessive moisture content will inhibit root growth and cause root rot. To improve the survival rate of leopard skin camphor cuttings in the wild, it is necessary to provide a suitable environment for the cuttings and meet their dynamic changing demand for water.

[0016] Therefore, the present invention adopts sodium alginate gel to coat vermiculite for many times and make gel-coated particles, be mixed into cutting medium, reach the purpose of dynamic water supply balance.What coating gel surface adopts is sodium alginate gel, has strong water absorption, can accommodate and fix a large amount of water in early stage and provide to cuttings, meet the water requirement of cuttings early stage, but if adopting sodium alginate gel alone, can cause action time shorter, can be degraded faster in soil, be difficult to maintain subsequent water supply, so vermiculite is coated inside gel, vermiculite has strong water retention, water permeability and fertilizer retention, be coated in inside and can when effectively retaining water, improve the stability of cutting medium in soil, extend the action time that moisture is provided in cutting medium. As time goes by after cutting, the water demand of the cuttings gradually decreases. At this time, the primary coating gel gradually degrades and releases ethyl glycolate. Ethyl glycolate enters the interlayer structure of vermiculite and reacts and combines with its internal metal ions to introduce hydrophobic sites, causing the water absorption and water retention of the vermiculite to gradually decrease. The secondary coating gel also gradually degrades at the same time, gradually exposing the vermiculite with gradually decreasing water absorption and water retention, thereby catering to the gradually decreasing water demand of the cuttings and better promoting their growth. At this time, the pores exposed by the vermiculite can increase the air permeability of the matrix.

[0017] When the coated particles are formed, the structure of the sodium alginate gel layer is relatively dense, which inhibits air circulation. Cuttings need sufficient oxygen to breathe and ensure survival. Therefore, 2,4-di-tert-butylphenol is added during the gel preparation process. This inhibits the bonding of sodium alginate molecules during the gel formation process, allowing the resulting gel to maintain a larger pore structure and increase the dissolved oxygen diffusion coefficient of the gel layer. This ensures both water supply and good oxygen circulation. The various components of the cutting medium work together to provide the cuttings with appropriate water, oxygen, and nutrients for growth, thereby improving the survival rate of cuttings in the field.

[0018] Furthermore, in step A, the mass ratio of 3 wt% sodium alginate solution, n-octanol, ethyl glycolate, 2,4-di-tert-butylphenol, and vermiculite is (20-30): (0.05-0.1): (0.5-1): (0.1-0.2): (15-25).

[0019] Furthermore, in step B, the mass ratio of 3 wt% sodium alginate solution, 2,4-di-tert-butylphenol, and gel primary coating particles is (40-60): (0.2-0.4): (20-30).

[0020] Furthermore, the mass ratio of the 3 wt % sodium alginate solution to the 10 wt % calcium chloride solution in step A and step B is (4-6):(1-2).

[0021] Furthermore, in step C, the mass ratio of the sterilized soil, coconut bran, humic acid and the water-absorbed gel secondary coated particles is (150-200): (6-15): (5-7): (40-60).

[0022] Furthermore, in step C, the water content is adjusted to 50-70%, and the pH is adjusted to 5.5-6.5.

[0023] Further, the rooting liquid components are:

[0024] 0.2-0.5mg / L indolebutyric acid + 0.5-1mg / L naphthaleneacetic acid + 30-40g / L sucrose, the rest is water.

[0025] Further, the cutting operation is specifically as follows:

[0026] The pretreated cuttings are planted directly into the soil at a depth of 2 / 3 of the cuttings, with leaves and buds exposed above the soil surface and leaves facing upwards. The soil is compacted after planting, with a plant spacing of 4-6 cm and a row spacing of 10-15 cm.

[0027] Beneficial effects:

[0028] 1. The present invention uses 2-3 year old leopard skin camphor tree seedling branches as cuttings for cuttings, effectively shortens the cutting rooting time and improves the survival rate. The cuttings obtained by cutting old tree fringe cuttings or the seedling branches of the seedlings formed by seed propagation are used as cuttings for cuttings, which increases the source of cuttings. After the fringe cuttings are collected, the seedlings form multi-branched seedlings and obtain more fringe cuttings for cuttings. This solves the problem of limited development of the leopard skin camphor tree industry caused by the shortage of fringe cutting resources when old tree branches are used for cuttings under the traditional method.

[0029] 2. The present invention collects cuttings from 2-3 year-old camphor tree seedlings to promote the branching of the seedlings to form multi-branched seedlings, which can effectively reduce the operating costs of pruning the camphor tree seedlings after planting. At the same time, compared with ordinary seedlings and cuttings with only 1-2 branches, the multi-branched seedlings of the present invention can increase the number of branches to 4-8, which can greatly increase the yield of camphor tree. The present invention simultaneously carries out cutting propagation and seedling cultivation of camphor tree, effectively reducing the production cost of camphor tree.

[0030] 3. The present invention prepares the cutting matrix and spreads it on the soil for cutting of Cinnamomum camphora, which can effectively reduce the problem of reduced survival rate of cuttings during field cutting due to poor resistance and sensitivity to the environment. Compared with the greenhouse cutting method, it effectively reduces the breeding cost and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 : This is a picture of multi-branched seedlings formed after the saplings of the leopard skin camphor tree of the present invention are collected;

[0032] Figure 2 : It is a single-branch seedling picture formed by not collecting fringe strips of the leopard-skin camphor tree seedling of the present invention. DETAILED DESCRIPTION

[0033] The present invention will be described in detail below with reference to specific embodiments and accompanying drawings:

[0034] The invention discloses a method for rapid propagation of Cinnamomum camphora by cuttings. Before carrying out the cutting propagation of Cinnamomum camphora, a cutting matrix needs to be prepared, as specifically described below.

[0035] Example 1: Preparation of cutting matrix

[0036] A: Prepare 25 kg of 3 wt% sodium alginate solution, add 0.08 kg of n-octanol, mix, and heat to 73°C with stirring for 1.5 hours. After the reaction is completed, cool to room temperature and add 0.15 kg of 2,4-di-tert-butylphenol and 20 kg of vermiculite with a particle size of 1 mm, and stir to mix evenly. Then, simultaneously add 0.8 kg of ethyl glycolate and 7 kg of 10 wt% calcium chloride solution, and stir to form a gel. After drying at 45°C, grind to a particle size of approximately 1.5 mm to obtain gel-coated particles.

[0037] B: Prepare 50 kg of 3 wt% sodium alginate solution, add 0.3 kg of 2,4-di-tert-butylphenol, stir well, and adjust the pH to 4.5. After heating to 55°C, add 25 kg of gel to coat the particles for the first time and mix well. Then add 15 kg of 10 wt% calcium chloride solution and stir to form a gel. Let it stand for 2 hours, dry at 45°C, and grind to a particle size of about 3 mm to obtain gel-coated particles for the second time.

[0038] C: Dig 180kg of sterilized soil, mix it with 10kg of coconut coir and 6kg of humic acid, adjust the moisture content to 60%, and adjust the pH to 6. Then put the gel secondary coated particles into water to fully absorb water, take 50kg and add it to mix, and then obtain the cutting medium.

[0039] Example 2: Preparation of cutting matrix II

[0040] A: Prepare 20 kg of a 3 wt% sodium alginate solution, add 0.05 kg of n-octanol, mix, and heat to 70°C with stirring for 2 hours. After the reaction is complete, cool to room temperature and add 0.1 kg of 2,4-di-tert-butylphenol and 15 kg of vermiculite with a particle size of 1 mm and stir to mix evenly. Then, simultaneously add 0.5 kg of ethyl glycolate and 5 kg of a 10 wt% calcium chloride solution and stir to form a gel. After drying at 45°C, grind to a particle size of approximately 1.5 mm to obtain primary gel-coated particles.

[0041] B: Prepare 40 kg of 3 wt% sodium alginate solution, add 0.2 kg of 2,4-di-tert-butylphenol, stir well, and adjust the pH to 4. After heating to 50°C, add 20 kg of gel to coat the particles for the first time and mix well. Then add 10 kg of 10 wt% calcium chloride solution and stir to form a gel. Let it stand for 1 hour, dry at 45°C, and grind to a particle size of about 3 mm to obtain gel-coated particles for the second time.

[0042] C: Dig 150kg of sterilized soil, mix it with 6kg of coconut coir and 5kg of humic acid, adjust the moisture content to 50%, and adjust the pH to 5.5. Then put the gel secondary coated particles into water to fully absorb water, take 40kg and add it to mix, and then obtain the cutting medium.

[0043] Example 3: Preparation of cutting matrix

[0044] A: 30 kg of 3 wt% sodium alginate solution was added with 0.1 kg of n-octanol and the mixture was heated to 75°C and stirred for 2 h. After the reaction was completed, the mixture was cooled to room temperature and 0.2 kg of 2,4-di-tert-butylphenol and 25 kg of vermiculite with a particle size of 1 mm were added and stirred to mix evenly. 1 kg of ethyl glycolate and 10 kg of 10 wt% calcium chloride solution were then added and stirred to form a gel. The mixture was dried at 45°C and ground to a particle size of approximately 1.5 mm to obtain primary gel-coated particles.

[0045] B: Prepare 60 kg of 3 wt% sodium alginate solution, add 0.4 kg of 2,4-di-tert-butylphenol, stir well, and adjust the pH to 5. After heating to 60°C, add 30 kg of gel to coat the particles for the first time and mix well. Then add 20 kg of 10 wt% calcium chloride solution and stir to form a gel. Let it stand for 2 hours, dry at 45°C, and grind to a particle size of about 3 mm to obtain gel-coated particles for the second time.

[0046] C: Dig 200kg of sterilized soil, mix it with 15kg of coconut coir and 7kg of humic acid, adjust the moisture content to 70%, and adjust the pH to 6. Then put the gel secondary coated particles into water to fully absorb water, take 60kg and add it to mix, and then obtain the cutting medium.

[0047] Comparative Example 1: Preparation of cutting matrix

[0048] In contrast to Example 1, the only difference is that n-octanol is not added in step A during the preparation of the cutting substrate in Comparative Example 1. Step A is as follows:

[0049] A: Prepare 25 kg of 3 wt% sodium alginate solution, add 0.15 kg of 2,4-di-tert-butylphenol and 20 kg of vermiculite with a particle size of 1 mm, and stir to mix. Then, simultaneously add 0.8 kg of ethyl glycolate and 7 kg of 10 wt% calcium chloride solution, and stir to form a gel. After drying at 45°C, grind to a particle size of approximately 1.5 mm to obtain gel-coated primary particles.

[0050] BC: Same as Example 1.

[0051] Comparative Example 2: Preparation of cutting matrix

[0052] In contrast to Example 1, the only difference is that ethyl glycolate is not added in step A during the preparation of the cutting matrix in Comparative Example 2, as shown below:

[0053] A: Prepare 25 kg of 3 wt% sodium alginate solution, add 0.08 kg of n-octanol, mix, and heat to 73°C with stirring for 1.5 hours. After the reaction is completed, cool to room temperature and add 0.15 kg of 2,4-di-tert-butylphenol and 20 kg of vermiculite with a particle size of 1 mm and stir to mix evenly. Then, add 7 kg of 10 wt% calcium chloride solution and stir to form a gel. After drying at 45°C, grind to a particle size of about 1.5 mm to obtain gel-coated particles.

[0054] BC: Same as Example 1.

[0055] Comparative Example 3: Preparation of cutting matrix

[0056] In contrast to Example 1, the only difference is that 2,4-di-tert-butylphenol is not added in Step A and Step B during the preparation of the cutting substrate in Comparative Example 3, as follows:

[0057] A: Prepare 25 kg of 3 wt% sodium alginate solution, add 0.08 kg of n-octanol, mix, and heat to 73°C with stirring for 1.5 hours. After the reaction is completed, cool to room temperature and add 20 kg of vermiculite with a particle size of 1 mm and stir to mix evenly. Then, simultaneously add 0.8 kg of ethyl glycolate and 7 kg of 10 wt% calcium chloride solution and stir to form a gel. After drying at 45°C, grind to a particle size of approximately 1.5 mm to obtain the primary gel-coated particles.

[0058] B: Prepare 50 kg of 3 wt% sodium alginate solution, adjust the pH to 4.5, heat to 55°C, add 25 kg of gel to coat the particles, mix well, then add 15 kg of 10 wt% calcium chloride solution and stir to form a gel. Let stand for 2 hours, dry at 45°C, and grind to a particle size of approximately 3 mm to obtain gel-coated particles.

[0059] C: Same as Example 1.

[0060] Comparative Example 4: Preparation of cutting matrix

[0061] In contrast to Example 1, the only difference is that in Comparative Example 4, when preparing the cutting substrate, the pH is not adjusted in step B. 2,4-di-tert-butylphenol is directly added, and then the temperature is increased and the gel is added to initially coat the particles, mixed, and then subsequent operations are performed, specifically as follows:

[0062] A: Same as Example 1;

[0063] B: Prepare 50 kg of 3 wt% sodium alginate solution, add 0.3 kg of 2,4-di-tert-butylphenol and mix well. After heating to 55°C, add 25 kg of gel to coat the particles for the first time and mix well. Then add 15 kg of 10 wt% calcium chloride solution and stir to form a gel. Let it stand for 2 hours, dry at 45°C, and grind to a particle size of about 3 mm to obtain gel-coated particles for the second time.

[0064] C: Same as Example 1.

[0065] Comparative Example 5: Preparation of cutting matrix

[0066] In contrast to Example 1, the only difference is that in Comparative Example 5, step A and step B are missing when preparing the cutting matrix, that is, the vermiculite is not coated, but the gel secondary coated particles are replaced with an equal amount of vermiculite, as follows:

[0067] 180 kg of sterilized soil was dug out, mixed with 10 kg of coconut bran and 6 kg of humic acid, and the water content was adjusted to 60%, the pH was adjusted to 6, and then 50 kg of vermiculite was added and mixed to obtain a cutting medium.

[0068] Embodiment 4: Cinnamomum camphora cutting rapid propagation method

[0069] The specific components of the rooting solution used in this example are: 0.3 mg / L indolebutyric acid + 1 mg / L naphthaleneacetic acid + 35 g / L sucrose, and the rest is water.

[0070] (1) Land preparation: On a sunny day, select loose, fertile, and relatively moist soil as the cutting site, plow it to a depth of about 30 cm, break up large clods, and spray it with 0.1% potassium permanganate solution to disinfect the soil after drying it for 2 days. Then, spread the cutting medium prepared in Example 1 to a thickness of about 7 cm.

[0071] (2) Cutting collection and processing: Select 2-year-old Cinnamomum camphora seedlings, cut the trunk and trim it to about 5 cm in length to obtain cuttings; spray the wound with 0.1% potassium permanganate solution for disinfection, and after 1 hour, place the cuttings in rooting solution and soak for 40 minutes to obtain pre-treated cuttings;

[0072] (3) Cuttings: In late September, pre-treated cuttings were inserted directly into the soil covered with cutting medium. The depth of the cuttings was 2 / 3 of the cuttings, with leaves and buds exposed above the soil surface and leaves facing upwards. The soil was compacted after insertion. The spacing between cuttings was about 5 cm and the spacing between rows was about 12 cm. The cuttings were subsequently maintained according to conventional methods.

[0073] Comparative Example 6: Rapid Propagation Method of Cinnamomum camphora

[0074] In contrast to Example 4, the only difference is that in Comparative Example 6, when performing rapid propagation of Cinnamomum camphora cuttings, in step (2), branches of 10-year-old Cinnamomum camphora trees are used as cuttings instead of branches of 2-year-old seedlings. The remaining steps are the same as in Example 4, as follows:

[0075] (1) Same as Example 4;

[0076] (2) Cutting collection and processing: Select 10-year-old Cinnamomum camphora mother tree, cut off strong branches and trim them to a length of about 5 cm to obtain cuttings; spray 0.1% potassium permanganate solution on the wound for disinfection, and after 1 hour, place the cuttings in rooting solution and soak for 40 minutes to obtain pre-treated cuttings;

[0077] (3) Same as Example 4.

[0078] Comparative Example 7: Rapid Propagation Method of Cinnamomum camphora

[0079] In contrast to Example 4, the only difference is that in Comparative Example 7, no rooting liquid was used for cutting of Cinnamomum camphora, and the cuttings were directly sterilized before cutting.

[0080] (1) Same as Example 4;

[0081] (2) Cutting collection and processing: Select 2-year-old Cinnamomum camphora seedlings, cut the trunk and trim it to about 5 cm in length to obtain cuttings; spray 0.1% potassium permanganate solution on the wound for disinfection to obtain pre-treated cuttings;

[0082] (3) Same as Example 4.

[0083] Comparative Example 8: Rapid Propagation Method of Cinnamomum camphora

[0084] In contrast to Example 4, the only difference is that in Comparative Example 8, no cutting medium was added when cuttings of Cinnamomum camphora were carried out, and the cuttings were directly carried out in the disinfected soil.

[0085] (1) Land preparation: On a sunny day, select loose, fertile and relatively moist soil as the cutting site, plow it to a depth of about 30 cm, break up large clods, and spray the soil with 0.1% potassium permanganate solution after drying it for 2 days to disinfect it;

[0086] (2) Same as Example 4;

[0087] (3) Cuttings: In late September, pre-treated cuttings were inserted directly into the disinfected soil at a depth of 2 / 3 of the cuttings, with leaves and buds exposed above the soil surface and leaves facing upwards. The soil was compacted after insertion. The spacing between cuttings was about 5 cm and the spacing between rows was about 12 cm. The cuttings were subsequently maintained according to conventional methods.

[0088] Experiment 1: Testing the water retention effect of cutting medium

[0089] The water retention of the substrates prepared in Example 1 and Comparative Examples 1-5 was tested using the following method:

[0090] Take 6 flowerpots of consistent specifications, poke 10 even small holes in the bottom, then take each 500g of the gel secondary coated particles prepared in Example 1 and Comparative Examples 1-4, and respectively pack them into the flowerpot after mixing with 2000g of soil (corresponding to Example 1 and Comparative Example 1-4 respectively); Take 500g of vermiculite with a particle diameter of 1mm, and also pack it into the flowerpot after mixing with 2000g of soil (corresponding to Comparative Example 5), then each slowly water 500g and be placed outdoors, weigh the gross weight (flowerpot weight is peeled) of each assembly equipped with the matrix flowerpot on the 2nd day, the 15th day, and the 30th day, judge each group of matrix water retention with this, and obtain data as shown in Table 1:

[0091] Table 1

[0092]

[0093]

[0094] According to the data analysis in Table 1, we can see that:

[0095] (1) In Example 1, the water retention is high on the second day after watering, and the water retention gradually decreases after 15 and 30 days. However, a large amount of water is required for cuttings during cutting. When callus tissue is formed after about 15 days and rooting begins after 30 days, the water requirement gradually decreases. The gel-coated particles prepared in Example 1 and mixed with soil can better meet the gradually decreasing dynamic water requirement of cuttings, which is beneficial to improving the survival rate of field cuttings;

[0096] (2) In Comparative Example 1, n-octanol was not added to treat the sodium alginate. In Example 1, n-octanol was added for treatment, which reduced the polarity of the primary coating gel, increased the affinity of the gel for ethyl glycolate, and released ethyl glycolate at a more uniform rate, resulting in a more uniform decrease in the water retention of the composite material. In Comparative Example 1, since n-octanol was not added for treatment, ethyl glycolate was released too quickly, resulting in a rapid decrease in its water retention performance.

[0097] (3) In Comparative Example 2, ethyl glycolate was not added, and it failed to enter the vermiculite to react with it to reduce its later water absorption, so the weight did not change significantly compared with Example 1; in Comparative Example 5, the vermiculite was not coated with gel, and the weight decreased significantly on the second day, indicating that the coating of the gel layer can effectively increase the water retention performance of the initial matrix, meeting the characteristics of high water demand in the initial stage of cuttings. The cutting matrix prepared according to the method of the present invention can effectively retain water and fertilizer and increase air circulation, while gradually reducing water retention over time, thereby reducing the water content of the soil at the root of the cuttings, better adapting to the dynamic changes in water demand during the rooting process of the leopard skin camphor cuttings, and thus improving its field cutting survival rate.

[0098] Experiment 2: Rapid propagation of Cinnamomum camphora by cuttings

[0099] A rapid propagation test of Cinnamomum camphora was conducted at the Geleshan Base of the Academy of Forestry. The cutting media prepared in Example 1 and Comparative Examples 1-5 were used to propagate Cinnamomum camphora. The experiment was divided into 9 groups: experimental group 1 and control groups 1-8. The cutting media and cutting methods used in each group were as follows:

[0100] Experimental Group 1: Using the cutting medium of Example 1 and the cutting method of Example 4;

[0101] Control groups 1-5: using the cutting media of comparative examples 1-5 and the cutting method of example 4 respectively;

[0102] Control groups 6-7: using the cutting methods of comparative examples 6-7 and the cutting medium of Example 1 respectively;

[0103] Control group 8: No cutting medium was used, and the cutting method of comparative example 7 was adopted.

[0104] The number of cuttings in each group was 30. The rooting time of each cutting and the survival rate after 90 days of cutting were calculated. The results were repeated three times and the data are shown in Table 2:

[0105] Table 2

[0106]

[0107] According to the data analysis in Table 2:

[0108] The rooting time and survival rate of the leopard skin camphor tree in the experimental group 1 were better than those in the control groups 1-8. The rooting time of the leopard skin camphor tree in the experimental group 1 was 25-31 days, and the survival rate could reach 90%. This shows that the use of the cutting medium of the present invention and the method of the present invention for cutting the leopard skin camphor tree can effectively improve the survival rate of the leopard skin camphor tree cuttings, shorten the rooting time, and the seedlings after collecting the cuttings have better formed multi-branched seedlings ( Figure 1 ), while the seedlings without cuttings failed to branch well ( Figure 2 ).

[0109] Due to the changes in raw materials or steps in the preparation of the cutting medium in control groups 1-5, the water retention, air permeability and other properties of the cutting medium were reduced to varying degrees, especially the water retention property failed to form a change process from high to low, resulting in a failure to better match the changes in the water demand of the cuttings, thereby causing varying degrees of reduction in the rooting time and survival rate; in control group 6, 10-year-old old branches were used for cuttings. The physiological state of old branches was different from that of 2-year-old seedling branches, which resulted in a decrease in the rooting rate and survival rate; in control group 7, no rooting solution was used for treatment, and the rooting time was significantly increased and the survival rate was significantly decreased, indicating that rooting solution treatment is an important factor in promoting the rooting and survival of Cinnamomum camphora cuttings; in control group 8, no cutting medium was used, and the soil condition in the field was poor, which failed to adapt to the physiological needs of the cuttings during the rooting process, resulting in a long rooting time and a significant decrease in the survival rate.

[0110] The above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art will appreciate that the technical solutions of the present invention may be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, and such modifications or equivalents shall be encompassed by the claims of the present invention. Any techniques, shapes, and structures not described in detail herein are well known.

Claims

1. A method for rapid propagation of Cinnamomum camphora seedlings by cuttings, characterized in that: The method is as follows: (1) Land preparation: plough and disinfect the soil, and spread the cutting medium on the surface of the disinfected soil; (2) Cutting collection and processing: Select 2-3 year old Cinnamomum camphora seedlings, cut off the upper part of the trunk and trim it to a length of 4-7 cm to obtain cuttings, disinfect the cut ends and soak them in rooting solution to obtain pre-treated cuttings; (3) Cutting: From September to October, the pre-treated cuttings are cut into the soil after the cutting medium is spread flat, and then maintained according to conventional methods; The cutting matrix preparation method is as follows: A: Prepare a 3wt% sodium alginate solution, add n-octanol, mix, and heat to 70-75°C with stirring for 1-2 hours. After the reaction is complete, cool to room temperature and add 2,4-di-tert-butylphenol and vermiculite, mix evenly. Then, add ethyl glycolate and 10wt% calcium chloride solution simultaneously, stir to form a gel, dry, and grind into granules to obtain gel-coated primary particles. B: Separately prepare a 3wt% sodium alginate solution, add 2,4-di-tert-butylphenol, stir well, and adjust the pH to 4-5. Heat to 50-60°C, add the gel to initially coat the particles, and mix well. Immediately add 10wt% calcium chloride solution and stir to form a gel. Let stand for 1-2 hours, then dry and grind into granules to obtain gel-coated particles. C: Mix the excavated detoxified soil with coconut bran and humic acid, adjust the moisture content and pH, add the fully water-absorbing gel secondary coating particles, and mix well to obtain the cutting medium.

2. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 1, wherein: The mass ratio of 3 wt% sodium alginate solution, n-octanol, ethyl glycolate, 2,4-di-tert-butylphenol, and vermiculite in step A is (20-30): (0.05-0.1): (0.5-1): (0.1-0.2): (15-25).

3. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 2, wherein: In the step B, the mass ratio of the 3 wt % sodium alginate solution, 2,4-di-tert-butylphenol, and the gel-coated particles is (40-60): (0.2-0.4): (20-30).

4. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 3, wherein: The mass ratio of the 3 wt % sodium alginate solution to the 10 wt % calcium chloride solution in step A and step B is (4-6): (1-2).

5. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 4, wherein: In the step C, the mass ratio of the sterilized soil, coconut bran, humic acid and the water-absorbed gel secondary coated particles is (150-200): (6-15): (5-7): (40-60).

6. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 5, wherein: In step C, the water content is adjusted to 50-70%, and the pH is adjusted to 5.5-6.

5.

7. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 1, wherein: The rooting liquid components are: 0.2-0.5mg / L indolebutyric acid + 0.5-1mg / L naphthaleneacetic acid + 30-40g / L sucrose, the rest is water.

8. The method for rapid propagation of Cinnamomum camphora seedlings by cuttings according to claim 7, characterized in that: The cutting operation is specifically as follows: The pretreated cuttings are planted directly into the soil at a depth of 2 / 3 of the cuttings, with leaves and buds exposed above the soil surface and leaves facing upwards. The soil is compacted after planting, with a plant spacing of 4-6 cm and a row spacing of 10-15 cm.

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