A method for processing Lepidium apetalum seeds using jujubes

Abstract

This invention discloses a method for processing Lepidium apetalum using jujubes, belonging to the field of traditional Chinese medicine processing technology. The steps include: a) taking Lepidium apetalum slices, washing, draining, and removing impurities, and setting aside; b) taking a certain weight of jujubes, adding water and decocting twice, combining the decoctions, and concentrating to obtain a jujube decoction; c) taking the Lepidium apetalum from step a, adding it to the jujube decoction from step b, mixing well, and allowing it to soak until the jujube decoction is completely absorbed; d) placing the soaked Lepidium apetalum slices from step c in a preheated wok and stir-frying until dry, thus obtaining various processed Lepidium apetalum products. When used, the processed Lepidium apetalum products obtained by this method can be prepared into different pharmaceutically acceptable dosage forms or combined with other drugs. The method is simple to operate, requires minimal equipment, and effectively reduces the adverse reactions of diarrhea caused by the cold nature of Lepidium apetalum while maintaining or enhancing its antiasthmatic, antitussive, and expectorant effects, showing great application potential.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine processing technology, specifically to a method for processing Lepidium apetalum seeds using jujubes. Background Technology

[0002] Lepidium seed has the effects of purging the lungs and relieving asthma, promoting diuresis and reducing swelling. However, due to its strong and cold nature, it can cause adverse reactions such as loose stools, increased frequency of bowel movements, and diarrhea due to spleen deficiency during medication. The *Zheng Lei Ben Cao* records that long-term use of Lepidium seed can cause weakness; the *Ben Cao Gang Mu* states that "Lepidium seed is of a fiery and rapid nature, and it is good at expelling water. Patients with even slight weakness should avoid it." Thus, the clinical application of Lepidium seed is greatly limited. Processing is one of the characteristics of traditional Chinese medicine. Proper processing can moderate the effects and enhance the efficacy of potent Chinese medicines. Jujube, with its sweet and warm properties, has the effect of tonifying the middle and replenishing qi. The *Zheng Lei Ben Cao* records that "children suffering from autumn dysentery should eat jujubes infused with insects, which is good." Ri Hua Zi says: "Dried jujubes moisten the heart and lungs, stop coughs, tonify the five internal organs, treat weakness and fatigue, remove intestinal gas, and when mixed with sesame powder, treat malnutrition and dysentery." Modern research shows that compound prescriptions containing jujubes, such as Huangqin Decoction, Fuling Guizhi Gancao Dazao Decoction, and Ganmai Dazao Decoction, play an important role in tonifying the middle jiao and stopping diarrhea. In folk medicine, walnut and jujube pills are used to treat early morning diarrhea with good results. This demonstrates the significant potential of jujubes in relieving diarrhea. The classic prescription Tingli Dazao Xiefei Decoction by the medical sage Zhang Zhongjing consists of two Chinese herbs: lepidium seed and jujube. This not only provides clinical support for their combined use but also provides a feasible basis for processing jujubes into lepidium seed. A textbook on traditional Chinese medicine edited by Zhong Gansheng and Yang Baican records that using jujubes and lepidium seed together can mitigate their toxicity. Furthermore, a decoction of lepidium seed and jujube has the effects of purging the lungs and relieving asthma, promoting diuresis and reducing swelling, clearing heat and resolving phlegm, tonifying the middle jiao and replenishing qi, nourishing blood and calming the mind, and is used to treat symptoms such as lung heat cough, excessive phlegm, and difficulty urinating. However, to date, there are no publicly available methods or processed slices of Lepidium apetalum made from jujubes, either domestically or internationally. This invention provides a method for processing Lepidium apetalum using jujubes, creating a new processed slice of Lepidium apetalum, in order to expand the scope of application of Lepidium apetalum slices and promote the safe and efficient application of Lepidium apetalum. Summary of the Invention

[0003] In view of the above situation and to overcome the defects of the existing technology, the purpose of this invention is to provide a method for processing Lepidium apetalum with jujubes, which can effectively improve the potent effects of Lepidium apetalum without reducing or enhancing its antiasthmatic, antitussive and expectorant effects, thus expanding its application prospects.

[0004] The technical solution provided by this invention is: a method for processing Lepidium apetalum seeds using jujubes, comprising the following steps:

[0005] a. Take the slices of Lepidium apetalum, wash them, drain them, remove impurities, and set aside.

[0006] b. Take jujubes, add 8-10 times their weight volume of water and decoct twice each time, combine the filtrates, concentrate, and obtain a jujube decoction with a mass concentration of 0.1-0.25 g / mL. The weight volume refers to the solid in g and the liquid in mL.

[0007] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed (about 30 minutes). The mass fraction ratio of Lepidium seed to jujube is 10:1 to 2.5 (the mass fraction ratio of Lepidium seed slices used to absorb the jujube decoction to the jujube decoction used is 10:1 to 2.5, the same below).

[0008] d. Place the moistened Lepidium seeds from step c into a preheated wok and stir-fry until dry. Remove and let cool to room temperature to obtain the processed Lepidium seed product made from jujubes.

[0009] The jujube-processed Lepidium seed product prepared by the above method is pulverized (ground into powder) into a powder, or after being ground into powder, it is sieved through a 150-mesh sieve (No. 8 sieve) and extracted by reflux with 95% ethanol by volume concentration to prepare a fluid extract, a pharmaceutically acceptable dosage form, or a traditional Chinese medicine composition composed of other traditional Chinese medicine components.

[0010] This invention utilizes abundant raw materials, employs a simple and easy-to-operate preparation method, and produces a product with high medicinal value. It not only significantly alleviates the problems of diarrhea and spleen deficiency caused by raw Lepidium apetalum, but also does not reduce or enhance its original antiasthmatic, antitussive, and expectorant effects. This demonstrates that the Lepidium apetalum decoction pieces of this invention have a safe and highly effective effect, improve the medicinal value of Lepidium apetalum, expand the medicinal value of Lepidium apetalum decoction pieces, have broad application prospects, make a creative contribution to the development of traditional Chinese medicine, and have significant social and economic benefits. Detailed Implementation

[0011] The following description is provided in conjunction with examples and specific implementation methods.

[0012] Example 1

[0013] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0014] a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities;

[0015] b. Take 5g of jujubes, add 50mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add another 40mL of water to the dregs, boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.1g / ml.

[0016] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:1 (i.e., 50g of Lepidium seed: 5g of jujube).

[0017] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and stir-fry for 20 minutes to dry. Remove and let cool to room temperature to obtain the processed Lepidium seed product made from jujubes.

[0018] Example 2

[0019] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0020] a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities;

[0021] b. Take 10g of jujubes, add 100mL of water and boil over high heat, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 90mL of water to the dregs, boil over high heat, then simmer over low heat for 30-40 minutes to obtain the second filtrate. Combine the two filtrates and concentrate to a jujube decoction with a mass concentration of 0.2g / ml.

[0022] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:2 (i.e., 50g of Lepidium seed: 10g of jujube).

[0023] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and dry for 20 minutes. Remove and let cool at room temperature to obtain the processed Lepidium seed product made from jujubes.

[0024] Example 3

[0025] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0026] a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities;

[0027] b. Take 12.5g of jujubes, add 125mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add another 125mL of water to the dregs, boil over high heat for the first time, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.25g / ml.

[0028] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:2.5 (i.e., 50g of Lepidium seed: 12.5g of jujube).

[0029] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and dry for 20 minutes. Remove and let cool at room temperature to obtain the processed Lepidium seed product made from jujubes.

[0030] Example 4

[0031] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0032] a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities;

[0033] b. Take 8g of jujubes, add 72mL of water and boil over high heat, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 64mL of water to the dregs, boil over high heat, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate to a jujube decoction with a mass concentration of 0.1g / ml.

[0034] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:1.6 (i.e., 50g of Lepidium seed: 8g of jujube).

[0035] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and dry for 20 minutes. Remove and let cool at room temperature to obtain the processed Lepidium seed product made from jujubes.

[0036] Example 5

[0037] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0038] a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities;

[0039] b. Take 9g of jujubes, add 90mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 72mL of water to the dregs, boil over high heat for the first time, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.2g / ml.

[0040] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:1.8 (i.e., 50g of Lepidium seed: 9g of jujube).

[0041] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and dry for 20 minutes. Remove and let cool at room temperature to obtain the processed Lepidium seed product made from jujubes.

[0042] Example 6

[0043] A method for processing Lepidium apetalum seeds using jujubes includes the following steps:

[0044] a. Take the slices of Lepidium apetalum, wash them, drain them, remove impurities, and set aside.

[0045] b. Take 25g of jujubes, add 250mL of water and boil over high heat, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 225mL of water to the dregs, boil over high heat, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.25g / ml.

[0046] c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed, and the mass ratio of Lepidium seed to jujube is 10:2.5 (i.e., 100g of Lepidium seed: 25g of jujube).

[0047] d. Place the moistened Lepidium seeds from step c into a preheated wok at 80°C and dry for 20 minutes. Remove and let cool at room temperature to obtain the processed Lepidium seed product made from jujubes.

[0048] The jujube-processed Lepidium seed slices prepared by the methods in Examples 1-6 above are pulverized (ground) into powder, or after being ground, they are sieved through a 150-mesh sieve (No. 8 sieve) and extracted by reflux with 95% ethanol (v / v) to prepare a fluid extract, or prepared into a pharmaceutically acceptable dosage form. Preferably, Examples 1-3 are powders and Examples 4-6 are fluid extracts, so that the same dosage form can be compared in the experiment. See the experimental data below for details.

[0049] The above are merely embodiments, used to illustrate specific implementation methods of the present invention, and not to limit the specific scope of protection of the present invention. Any amount of jujube-processed Lepidium seed slices required for medical use can be industrially produced according to actual needs.

[0050] This invention provides a process for processing Lepidium apetalum into medicinal slices using jujubes. This method is simple to operate, requires minimal equipment, and produces processed products that effectively reduce the adverse effects of diarrhea caused by the cold nature of Lepidium apetalum while maintaining or enhancing its anti-asthmatic, antitussive, and expectorant effects. This expands the medicinal value of Lepidium apetalum slices, and the experimental results demonstrate excellent therapeutic effects. Relevant experimental data are as follows:

[0051] 1. Set up experimental samples

[0052] Specifically: Example 1 is Sample No. 1; Example 2 is Sample No. 2; Example 3 is Sample No. 3; Example 4 is Sample No. 4; Example 5 is Sample No. 5; Example 6 is Sample No. 6; Take raw semen lepidii, remove impurities, powder it to obtain Sample No. 7; Take processed semen lepidii slices, remove impurities, powder them, and reflux extract with ethanol with a volume concentration of 95% to prepare a fluid extract to obtain Sample No. 8; Take Chinese dates, remove impurities, add 8 - 10 times the amount of water each time, decoct twice, combine the filtrates, and concentrate to a water decoction of Chinese dates with a mass concentration of 0.25 g / ml to obtain Sample No. 9.

[0053] 2. Experimental animals

[0054] 100 SPF - level male KM mice, weighing 16 - 18 g, were provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd. [SCXK(Lu)20220006].

[0055] 3. Observation or determination of the effects of processed semen lepidii on diarrhea, medicinal properties, and anti - asthma, cough - relieving, and expectorant effects

[0056] 3.1 Animal grouping and administration

[0057] After 100 male KM mice were adaptively fed for one week, they were randomly divided into a control group, Sample No. 1 group, Sample No. 2 group, Sample No. 3 group, Sample No. 4 group, Sample No. 5 group, Sample No. 6 group, Sample No. 7 group, Sample No. 8 group, and Sample No. 9 group according to body weight, with a total of 10 groups and 10 mice in each group. According to the previous research results, semen lepidii and each processed product group were administered by gavage at a dose of 20 g / kg, and the water decoction of Chinese dates was administered by gavage at a dose of 5 g / kg, once a day for 7 consecutive days. The control group was given an equal volume of 0.5% sodium carboxymethylcellulose solution. One hour after the last administration, the diarrhea index was measured to evaluate the change in medicinal properties and anti - asthma, cough - relieving, and expectorant effects.

[0058] 3.2 Determination of diarrhea index

[0059] The diarrhea index is the product of the loose stool rate and the loose stool grade. One hour after the last administration, one mouse was placed in each cage, and qualitative filter paper was placed in the cage. The feces were collected on the filter paper, and the filter paper was replaced every 0.5 h. The defecation situation was observed and recorded, and the number of loose stools and the loose stool grade of the mice within 4 h were recorded. Among them, the loose stool grade was determined according to the size of the stain area formed by the loose stool contaminating the qualitative filter paper, and the standard is shown in Table 1.

[0060] Table 1 Judgment criteria for loose stool levels

[0061] Diameter of stains on filter paper surface / cm loose stool level ＜1 1 1-1.9 2 2-2.9 3 3-3.9 4 4-4.9 5

[0062] 3.3 Monitoring of medicinal properties

[0063] 3.3.1 Anal temperature

[0064] One hour after the last administration of the drug to the mice, their rectal temperature was measured. During measurement, a small amount of glycerin lubricant was applied to the rectal thermometer, and then the metal tip of the thermometer was gently and slowly inserted into the mouse's anus to a depth of approximately 1 cm. The reading was taken after the temperature stabilized; this reading is the mouse's rectal temperature.

[0065] 3.3.2 Hot and cold plate test

[0066] The temperature directional behavior of mice was monitored using the hot-and-cold plate differential assay. The parameters of the hot-and-cold plate animal behavior differential assay instrument were set as follows: low temperature plate 20℃, high temperature plate 39℃. One hour after the last administration, mice from each group were placed sequentially into the respective channels of the hot-and-cold plates in a quiet environment. Their temperature directional activity was remotely monitored via camera, and the activity was recorded over 10 minutes, including the proportion of hot zones, the distance between zones, and the number of times the mice crossed zones. Alcohol was sprayed after each use to eliminate interference from mouse odor.

[0067] 3.4 Determination of Asthma Relief Indicators (Histamine Phosphate Combined with Acetylcholine Chloride Test)

[0068] Mice were placed in an asthma induction chamber and treated with an equal volume mixture of 2% acetylcholine chloride and 0.1% histamine phosphate at a constant pressure of 400 mmHg (1 mmHg ≈ 0.133 kPa) and a pressure of 10 mL / min. -1 Concentrated ammonia solution was sprayed evenly into the chamber in a mist form over a period of 15 seconds. The time from the start of spraying to the appearance of wheezing was observed as the wheezing latency period. The wheezing latency period and the number of wheezes were observed within 6 minutes.

[0069] 3.5 Determination of indicators related to antitussive efficacy (ammonia-induced cough test)

[0070] Invert a 500mL beaker onto a tabletop containing 10cm×10cm qualitative filter paper. Use a 1mL syringe to inject 0.1mL of concentrated ammonia onto the filter paper. After allowing it to evaporate naturally for 30 seconds, quickly remove the filter paper and place the beaker into the table. Observe the mouse for signs of coughing, such as abdominal muscle contraction, chest retraction, and mouth opening accompanied by coughing sounds. Record the number of coughs within 2 minutes and the time of the first cough.

[0071] 3.6 Determination of expectorant efficacy-related indicators (tracheal phenol red excretion test)

[0072] Mice were intraperitoneally injected with a 5% phenol red saline solution. Thirty minutes later, they were euthanized by cervical dislocation. Tracheal tissue of the same length from the thyroid cartilage to the tracheal branch was collected and placed in a centrifuge tube containing 2 mL of saline solution. 0.1 mL of 0.5% sodium bicarbonate solution was added, and the mixture was sonicated for 15 minutes and soaked for 24 hours. The tube was then centrifuged at 3000 rpm for 15 minutes, and the supernatant was collected. The absorbance was measured at 546 nm, and the results were used to calculate the amount of phenol red excreted from the mouse trachea using a phenol red standard curve (final results are expressed as concentration).

[0073] 4. Results

[0074] 4.1 The use of freshly prepared Lepidium apetalum slices made from jujubes to improve diarrhea in mice

[0075] The diarrhea index is a commonly used method to quantify the severity of diarrhea. Compared with the control group, the diarrhea index of mice in sample groups 7 and 8 was significantly increased, indicating that raw Lepidium apetalum caused diarrhea in mice. Compared with sample group 7, after processing with jujubes of different mass fractions (Lepidium apetalum / jujube = 10:2.5–10:1), the diarrhea index of mice in sample groups 1-3 was significantly reduced by 88.4%, 96.4%, and 98.8%, respectively (P < 0.01, P < 0.01, P < 0.01); compared with sample group 8, the diarrhea index of mice in sample groups 4-6 was significantly reduced by 92.1%, 97.6%, and 98.8%, respectively (P < 0.01, P < 0.01, P < 0.01), indicating that processing with jujubes can significantly alleviate diarrhea caused by Lepidium apetalum. The results are shown in Table 2.

[0076] Table 2. Effects of jujube-processed Lepidium apetalum on the diarrhea index in mice. n=10)

[0077] Group Diarrhea Index Relative rate of change (%) control group 0 - Sample group 7 <![CDATA[1.65±0.13 ** ]]> - Sample Group 1 <![CDATA[0.19±0.03 ## ]]> 88.4 Sample group 2 <![CDATA[0.06±0.01 ## ]]> 96.4 Sample group 3 <![CDATA[0.02±0.01 ## ]]> 98.8 Sample group No. 8 <![CDATA[1.65±0.10 ** ]]> - Sample group 4 0.13±0.02^^ 92.1 Sample group 5 0.04±0.03^^ 97.6 Sample group 6 0.02±0.00^^ 98.8 Sample group 9 0 -

[0078] Note: Compared with the control group, * P < 0.05 ** P < 0.01; compared with sample group 7, # P < 0.05 ## P < 0.01; compared with sample group 8, ^P < 0.05, ^^P < 0.01; relative change rate (%): compared with sample group 7 for sample groups 1-3, and compared with sample group 8 for sample groups 4-6.

[0079] 4.2 Processing Lepidium apetalum with jujubes can mitigate its extremely cold medicinal properties.

[0080] Rectal temperature and hot / cold plate tests are the main methods for reflecting the cold and hot properties of traditional Chinese medicine. Compared with the control group, the rectal temperature of mice in sample groups 7 and 8 was significantly lower (P < 0.01), and the proportion of mice staying in the hot zone was significantly higher (P < 0.01), indirectly reflecting the extremely cold nature of Lepidium apetalum. After processing with jujube, the rectal temperature of mice in each sample group (e.g., samples 1 to 6) increased (P < 0.01), and the proportion staying in the hot zone decreased (P < 0.01), indicating that the warming properties of jujube processing alleviated the extremely cold nature of Lepidium apetalum. The results are shown in Table 3.

[0081] Table 3. Effects of Jujube Processing on the Medicinal Properties of Lepidium apetalum (or Lepidium apetalum) n=10)

[0082] Group Rectal temperature Percentage of stay in hot zones (%) control group 36.53±0.23 35.72±0.63 Sample group 7 <![CDATA[35.13±0.28 ** ]]> <![CDATA[59.92±1.40 ** ]]> Sample Group 1 <![CDATA[35.95±0.36 ## ]]> <![CDATA[39.65±1.01 ## ]]> Sample group 2 <![CDATA[36.34±0.37 ## ]]> <![CDATA[37.61±0.84 ## ]]> Sample group 3 <![CDATA[36.44±0.27 ## ]]> <![CDATA[36.72±0.73 ## ]]> Sample group No. 8 <![CDATA[35.21±0.25 ** ]]> <![CDATA[57.50±1.12 ** ]]> Sample group 4 35.98±0.23^^ <![CDATA[37.83±0.70 ## ]]> Sample group 5 36.23±0.24^^ <![CDATA[37.02±0.61 ## ]]> Sample group 6 36.4±0.19^^ <![CDATA[36.15±0.99 ## ]]> Sample group 9 36.60±0.21 34.04±0.96

[0083] Note: Compared with the control group, * P < 0.05 ** P < 0.01; compared with sample group 7, # P < 0.05 ## P < 0.01; compared with sample group 8, ^P < 0.05, ^^P < 0.01.

[0084] 4.3 Processing jujubes with Lepidium apetalum does not reduce or enhance the antiasthmatic, antitussive, and expectorant effects of Lepidium apetalum.

[0085] 4.3.1 Processing jujubes into fresh slices of Lepidium apetalum does not reduce the antiasthmatic efficacy of Lepidium apetalum.

[0086] The histamine phosphate combined with acetylcholine chloride-induced asthma method in mice is a commonly used method for evaluating bronchodilator efficacy. Prolonging the latency period of wheezing and reducing the number of wheezes after administration reflects the bronchodilator efficacy of the drug. Compared with sample group 7, there were no significant differences in the latency period and number of wheezes in mice treated with the crude powder of each processed jujube product (e.g., samples 1 to 3) (P > 0.05); similarly, compared with sample group 8, there were no significant differences in the latency period and number of wheezes in mice treated with the extracts of each processed jujube product (e.g., samples 4 to 6) (P > 0.05). This indicates that the bronchodilator efficacy of Lepidium apetalum is not reduced after processing. The results are shown in Table 4.

[0087] Table 4. Indicators of asthma relief in mice before and after intervention with jujube-processed Lepidium apetalum slices ( n=10)

[0088] Group Breathing incubation period Relative rate of change (%) Number of breaths Relative rate of change (%) control group 48.10±1.50 - 133.40±3.24 - Sample group 7 75.40±2.43 - 103.40±2.76 - Sample Group 1 80.00±3.07 6.1 106.20±2.07 2.7 Sample group 2 83.80±1.76 11.1 102.60±1.83 0.8 Sample group 3 85.80±1.69 13.4 107.10±2.47 3.6 Sample group No. 8 70.40±2.12 - 105.80±2.58 - Sample group 4 73.70±2.67 4.7 107.20±2.03 1.3 Sample group 5 77.30±3.29 9.8 112.10±3.05 6.0 Sample group 6 79.60±1.66 13.1 104.60±2.17 1.1 Sample group 9 52.70±1.65 - 128.10±2.58 -

[0089] Note: Relative change rate (%): Comparison of sample groups 1-3 with sample group 7, and comparison of sample groups 4-6 with sample group 8.

[0090] 4.3.2 Processing jujubes into new slices of Lepidium apetalum enhances the antitussive effect of Lepidium apetalum.

[0091] The ammonia-induced cough test is a commonly used experiment to test the antitussive efficacy of drugs. If the drug can prolong the cough latency period and reduce the number of coughs induced by ammonia in mice after administration, it reflects the antitussive efficacy of the drug. Compared with sample group 7, the cough latency periods of each processed product (e.g., samples 1 to 3) increased to varying degrees, with increases of 7.5%, 15.4%, and 18.7% respectively. The number of coughs decreased in all samples, with decreases of 15.8%, 30.4%, and 51.9% respectively. The cough latency period of sample group 3 was significantly increased (P < 0.01), while the number of coughs in sample groups 2 and 3 was significantly decreased (P < 0.05; P < 0.01). Similarly, compared with sample group 8, the cough latency period of each processed product (e.g., samples 4 to 6) increased to varying degrees, with increases of 7.6%, 16.0%, and 21.6% respectively. The number of coughs decreased in all samples, with decreases of 14.9%, 30.9%, and 32.7% respectively. Sample groups 5 and 6 showed significantly increased cough latency periods (P < 0.05; P < 0.01) and significantly decreased cough frequency (P < 0.01). This indicates that the antitussive efficacy of Lepidium apetalum is enhanced after processing. The results are shown in Table 5.

[0092] Table 5. Cough-relieving indices in mice before and after intervention with jujube-processed Lepidium apetalum decoction pieces ( n=10)

[0093] Group Cough incubation period Relative rate of change (%) Number of coughs Relative rate of change (%) control group 60.30±1.96 - 44.10±0.97 - Sample group 7 75.90±1.65 - 32.20±1.15 - Sample Group 1 81.60±2.10 7.5 27.10±1.84 15.8 Sample group 2 87.60±2.93 15.4 <![CDATA[22.40±1.96 # ]]> 30.4 Sample group 3 <![CDATA[90.10±2.54 ## ]]> 18.7 <![CDATA[18.50±1.67 ## ]]> 51.9 Sample group No. 8 73.30±1.87 - 33.60±1.38 - Sample group 4 78.90±1.91 7.6 28.60±1.23 14.9 Sample group 5 85.00±1.92^ 16.0 23.20±1.13^^ 30.9 Sample group 6 89.10±2.08^^ 21.6 22.60±1.86^^ 32.7 Sample group 9 73.00±1.71 - 34.90±1.24 -

[0094] Note: Compared with sample group 7. # P < 0.05 ## P < 0.01; compared with sample group 8, ^P < 0.05, ^^P < 0.01; relative change rate (%): compared with sample group 7 for sample groups 1-3, and compared with sample group 8 for sample groups 4-6.

[0095] 4.3.3 Processing jujubes into fresh Lepidium apetalum slices does not reduce the expectorant efficacy of Lepidium apetalum.

[0096] The tracheal phenol red excretion test is a commonly used experiment to test the expectorant efficacy of drugs. The amount of phenol red excreted from the trachea of ​​mice after administration reflects the expectorant efficacy of the drug. Compared with sample group 7, there was no significant difference in the amount of phenol red excreted by mice treated with the crude powder of each processed jujube product (e.g., samples 1 to 3) (P > 0.05); similarly, compared with sample group 8, there was no significant difference in the amount of phenol red excreted by mice treated with the extracts of each processed jujube product (e.g., samples 4 to 6) (P > 0.05). This indicates that the expectorant efficacy of Lepidium apetalum is not reduced after processing. The results are shown in Table 6.

[0097] Table 6. Expectorant indices in mice before and after intervention with jujube-processed Lepidium apetalum slices ( n=10)

[0098] Group Phenol red excretion Relative rate of change (%) control group 0.51±0.03 - Sample group 7 1.06±0.03 - Sample Group 1 1.10±0.02 3.8 Sample group 2 1.14±0.02 7.5 Sample group 3 1.15±0.03 8.5 Sample group No. 8 0.85±0.03 - Sample group 4 0.90±0.02 5.9 Sample group 5 0.95±0.02 11.8 Sample group 6 0.96±0.02 12.9 Sample group 9 0.64±0.03 -

[0099] Note: Relative change rate (%): Comparison of sample groups 1-3 with sample group 7, and comparison of sample groups 4-6 with sample group 8.

[0100] The results above show that the Lepidium seed slices obtained by using this invention can not only significantly alleviate the problems of diarrhea and spleen deficiency caused by raw Lepidium seed, but also do not reduce or enhance its original antiasthmatic, antitussive, and expectorant effects. This indicates that the Lepidium seed slices of this invention have a safe and effective effect, improve the medicinal value of Lepidium seed, and make a creative contribution to the development of traditional Chinese medicine.

[0101] This invention provides a process for processing Lepidium apetalum into new medicinal slices using jujubes. For the first time, jujubes, a traditional Chinese medicine that is both food and medicine, are selected as the auxiliary material for processing. The method is simple to operate and requires low equipment. The processed products can effectively reduce the adverse reaction of diarrhea caused by the cold nature of Lepidium apetalum, while not reducing or enhancing its antiasthmatic, antitussive, and expectorant effects, and have good application prospects.

[0102] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for processing Lepidium apetalum seeds using jujubes, characterized in that, Includes the following steps: a. Take the slices of Lepidium apetalum, wash them, drain them, and remove any impurities; b. Take jujubes, add 8-10 times their weight volume of water and decoct twice each time, combine the filtrates, concentrate, and obtain a jujube decoction with a mass concentration of 0.1~0.25 g / mL; c. Take the Lepidium seed slices from step a, add them to the jujube decoction from step b, mix well, let it sit until the jujube decoction is completely absorbed. d. Place the moistened Lepidium seeds from step c into a preheated wok and stir-fry until dry. Remove and let cool to room temperature to obtain the processed Lepidium seed product made from jujubes. The mass fraction ratio of the Lepidium seed to the jujube is 10:1~2.5; In step b, the mass concentration of the jujube decoction is 0.25 g / ml; In step d, the process of frying until dry is described as frying at 80 °C for 20 min.

2. The method according to claim 1, characterized in that, In step b, the method for preparing the jujube decoction is as follows: Take 5g of jujube, add 50mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add another 40mL of water to the dregs, boil over high heat, then simmer over low heat for 30-40 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.1g / ml. The mass ratio of Lepidium apetalum to jujube is 10:

1.

3. The method according to claim 1, characterized in that, In step b, the method for preparing the jujube decoction is as follows: Take 10g of jujube, add 100mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 90mL of water to the dregs, boil over high heat, then simmer over low heat for 30-40 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.2g / ml; and the mass fraction ratio of Lepidium apetalum to jujube is 10:

2.

4. The method according to claim 1, characterized in that, In step b, the method for preparing the jujube decoction is as follows: Take 8g of jujubes, add 72mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 64mL of water to the dregs, boil over high heat, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.1g / ml. The mass fraction ratio of Lepidium apetalum to jujubes is 10:1.

6.

5. The method according to claim 1, characterized in that, In step b, the method for preparing the jujube decoction is as follows: Take 9g of jujubes, add 90mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 72mL of water to the dregs, boil over high heat, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.2g / ml; and the mass fraction ratio of Lepidium apetalum to jujube is 10:1.

8.

6. The method according to claim 1, characterized in that, In step b, the method for preparing the jujube decoction is as follows: Take 25 g of jujubes, add 250 mL of water and boil over high heat for the first time, then simmer over low heat for 30-40 minutes to obtain the first filtrate. Add 225 mL of water to the dregs, boil over high heat, then simmer over low heat for 30-35 minutes to obtain the second filtrate. Combine the two filtrates and concentrate them to a jujube decoction with a mass concentration of 0.25 g / mL. The mass fraction ratio of Lepidium apetalum to jujubes is 10:2.

5.

7. The use of the jujube-processed Lepidium seed product prepared by any one of claims 1-6 in the preparation of powders.

8. The application of the processed jujube extract prepared by any one of claims 1-6 in the preparation of fluid extract; characterized in that, The application includes: grinding the processed product into powder, passing it through a 150-mesh sieve, and extracting it by reflux with 95% ethanol to prepare a fluid extract.